CN217578892U - Cell transfer device in stem cell detection device - Google Patents
Cell transfer device in stem cell detection device Download PDFInfo
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- CN217578892U CN217578892U CN202221313731.8U CN202221313731U CN217578892U CN 217578892 U CN217578892 U CN 217578892U CN 202221313731 U CN202221313731 U CN 202221313731U CN 217578892 U CN217578892 U CN 217578892U
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- transfer device
- stem cell
- cell
- cell transfer
- separation device
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- 210000004027 cell Anatomy 0.000 title claims abstract description 34
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 28
- 238000012546 transfer Methods 0.000 title claims abstract description 24
- 238000001514 detection method Methods 0.000 title claims abstract description 18
- 238000000926 separation method Methods 0.000 claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 238000010521 absorption reaction Methods 0.000 claims abstract description 9
- 238000005070 sampling Methods 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 2
- 238000003556 assay Methods 0.000 claims 6
- 239000007787 solid Substances 0.000 description 8
- 239000001963 growth medium Substances 0.000 description 7
- 239000007788 liquid Substances 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 239000000306 component Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000001778 pluripotent stem cell Anatomy 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 210000003014 totipotent stem cell Anatomy 0.000 description 1
- 210000002444 unipotent stem cell Anatomy 0.000 description 1
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Abstract
The utility model discloses a cell transfer device in a stem cell detection device, which comprises a cell transfer device body, wherein the body is in a hollow conical shape with a thick upper part and a thin lower part, and two ends are provided with clamping connection parts; the upper end of the body is connected with a water absorption ball in a clamping manner, the lower end of the body is connected with a separation device in a clamping manner, the separation device is in a hollow conical shape with a thick upper part and a thin lower part, clamping connection parts are arranged at two ends of the separation device, and a filter layer is arranged in a hollow inner cavity; the lower end of the separation device is connected with a community processor in a clamping way. The utility model discloses can realize adsorbing and transporting the stem cell under the different culture state, improve the efficiency and the detection accuracy that detect.
Description
Technical Field
The utility model belongs to the technical field of detect, concretely relates to cell transfer ware among stem cell detection device.
Background
Stem cells are a type of pluripotent cells with self-renewal, which under certain conditions can differentiate into multiple functional cells. The stem cells are classified into three categories according to their developmental potential: totipotent, pluripotent and unipotent stem cells. The stem cells provide seed cells for tissue engineering application and are effective tools for exploring mysterious human development, and have great prospects in basic research and clinical application.
When stem cells are detected, the existing method generally adopts an electron microscope for observation, the existing detection device has high automation degree, can automatically sample from cell cultures, and transfers the samples to a sample bearing device on an objective table for detection, and the method has high automation degree and can carry out high-throughput detection. However, because the culture medium components of the cell culture are different, and the design of the sampling needle at present is only suitable for stem cells of liquid culture, the solid and semisolid culture is difficult to use because the stripping and the suction of the cell colony are involved, and because the cells grow on the solid or semisolid culture medium, the culture medium components are easily brought into the sampler during the stripping and the suction and then transferred to the sample carrying device for detection, and the detection effect is influenced; or because the current inner pipeline of the sampling needle is a capillary, the capillary pipeline is easily blocked by the components of the culture medium, so that the transportation of the stem cells is influenced. Therefore, there is a need to overcome this drawback.
Disclosure of Invention
The utility model discloses the technical problem that will solve is: the cell transfer device in the stem cell detection device overcomes the defects in the prior art, can realize the adsorption and transfer of stem cells in different culture states, and improves the detection efficiency and the detection accuracy.
In order to solve the technical problem, the technical scheme of the utility model is that:
a cell transfer device in a stem cell detection device comprises a cell transfer device body, wherein the cell transfer device body is in a hollow conical shape with a thick upper part and a thin lower part, and two ends of the cell transfer device body are provided with clamping connection parts; the upper end of the body is connected with a water absorption ball in a clamping manner, the lower end of the body is connected with a separation device in a clamping manner, the separation device is in a hollow conical shape with a thick upper part and a thin lower part, clamping connection parts are arranged at two ends of the separation device, and a filter layer is arranged in a hollow inner cavity; the lower end of the separation device is connected with a community processor in a clamping way.
Preferably, the joints of the clamping connection parts at the two ends of the body are all frosted. The strength and the sealing performance of the clamping connection combination are improved.
Preferably, the upper end of the body can be plugged into a sampling mechanism in the stem cell detection device. Automatic identification is performed and sampling is assisted.
Preferably, the joints of the clamping connection parts at the two ends of the separating device are all arranged in a frosted mode.
Preferably, the filtering layer is a cell filter screen. U.S. BD FALCON cell screens, cell screens with 40, 70 or 100um holes can be selected according to requirements.
Preferably, the community treater include hollow lower extreme open-ended cylindric main part, main part upper portion central authorities intercommunication have the joint body, the joint body is hollow thick cone down, the internal surface of joint body is the dull polish setting.
Preferably, the body, the water absorption ball, the separation device and the community processor are all disposable sterile equipment.
Since the technical scheme is used, the beneficial effects of the utility model are that:
the utility model discloses a ball and community treater absorb water will handle liquid (enzyme liquid etc.) location and wash the cell community, then aspirate the cell and transfer the ware in, again effectual solid medium impurity of having avoided enters into the cell and transfers the ware through the filter layer, consequently, can the efficient accomplish the transportation of solid, semi-solid stem cell of cultivateing to guaranteed the efficiency that detects and detect the accuracy, also can transport other matrixes again and accomplish the cultivateing again of stem cell.
Drawings
Fig. 1 is a schematic structural diagram of an embodiment of the present invention;
fig. 2 is an exploded view of the embodiment of the present invention;
FIG. 3 is a schematic view of a prior art sampling needle;
in the figure, 1, a body; 2. a water-absorbing ball; 3. a separation device; 4. a filter layer; 5. a community processor; 51. a main body; 52. a clamping body.
Detailed Description
The invention is further explained below with reference to the drawings and examples.
Example one
As shown in fig. 1 to 3, a cell transfer device in a stem cell detection device comprises a body 1 of the cell transfer device, wherein the body 1 is a hollow cone with a thick upper part and a thin lower part, and two ends are provided with clamping connection parts; the upper end of the body 1 is connected with a water absorption ball 2 in a clamping manner, the lower end of the body 1 is connected with a separation device 3 in a clamping manner, the separation device 3 is in a hollow conical shape with a thick upper part and a thin lower part, clamping connection parts are arranged at two ends of the separation device, and a filter layer 4 is arranged in a hollow inner cavity; the lower end of the separation device 3 is connected with a community processor 5 in a clamping way; the community treater 5 comprises a hollow cylindrical main body 51 with an opening at the lower end, the center of the upper part of the main body 51 is communicated with a clamping body 52, the clamping body 52 is a hollow conical body with a thick upper part and a thin lower part, and the inner surface of the clamping body is arranged in a frosted mode.
In practical application, taking the example of sucking the cell colony on the solid culture medium, taking a disposable sterile body 1, a water absorption ball 2 and a separation device 3 for insertion, using the water absorption ball 2 to fully suck the enzyme preparation or a corresponding solvent (selected according to actual needs) in the body 1 after the insertion, then inserting a colony processor 5, buckling the colony processor 5 on the cell colony of the solid culture medium, slightly pressing the colony processor 5 to enable the lower edge of the colony processor 5 to be tightly combined with the surface of the solid culture medium so as to ensure no liquid leakage, injecting the enzyme preparation or the corresponding solvent into the colony processor 5, using the water absorption ball 2 to suck so that the enzyme preparation or the corresponding solvent washes the cultured stem cells, then sucking the liquid mixed with the stem cells into the body 1 through the water absorption ball 2, because of the separation effect of the separation device 3, impurities cannot enter the body 1, discharging the colony processor 5, transferring the colony processor 5 to an area to be detected, dropping the liquid of the stem cells to the detection area, and discarding or transferring the residual culture.
It should be understood that these examples are only for illustrating the present invention and are not intended to limit the scope of the present invention. Furthermore, it should be understood that various changes and modifications of the present invention may be made by those skilled in the art after reading the teachings of the present invention, and these equivalents also fall within the scope of the appended claims.
Claims (6)
1. A cell transfer device in a stem cell assay device, comprising: the cell transfer device comprises a body of the cell transfer device, wherein the body is in a hollow conical shape with the thick upper part and the thin lower part, and two ends of the body are provided with clamping connection parts; the upper end of the body is connected with a water absorption ball in a clamping manner, the lower end of the body is connected with a separation device in a clamping manner, the separation device is in a hollow conical shape with a thick upper part and a thin lower part, clamping connection parts are arranged at two ends of the separation device, and a filter layer is arranged in a hollow inner cavity; the lower end of the separation device is connected with a community processor in a clamping manner.
2. A cell transfer device in a stem cell assay device according to claim 1, wherein: the junction of the joint connecting parts at the two ends of the body is frosted.
3. A cell transfer device in a stem cell assay device according to claim 1, wherein: the upper end of the body can be plugged into a sampling mechanism in the stem cell detection device.
4. A cell transfer device in a stem cell assay device according to claim 1, wherein: the junction of the joint connecting parts at the two ends of the separating device is frosted.
5. A cell transfer device in a stem cell assay device according to claim 1, wherein: the filtering layer is a cell filter screen.
6. A cell transfer device in a stem cell assay device according to claim 1, wherein: the community treater includes the cylindric main part of hollow lower extreme open-ended, main part upper portion central authorities intercommunication has the joint body, and the joint body is hollow thick-end-up's toper body, the internal surface of joint is the dull polish setting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221313731.8U CN217578892U (en) | 2022-05-27 | 2022-05-27 | Cell transfer device in stem cell detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221313731.8U CN217578892U (en) | 2022-05-27 | 2022-05-27 | Cell transfer device in stem cell detection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217578892U true CN217578892U (en) | 2022-10-14 |
Family
ID=83528688
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221313731.8U Active CN217578892U (en) | 2022-05-27 | 2022-05-27 | Cell transfer device in stem cell detection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217578892U (en) |
-
2022
- 2022-05-27 CN CN202221313731.8U patent/CN217578892U/en active Active
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