CN213113292U - Nucleic acid extraction structure - Google Patents
Nucleic acid extraction structure Download PDFInfo
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- CN213113292U CN213113292U CN202021605586.1U CN202021605586U CN213113292U CN 213113292 U CN213113292 U CN 213113292U CN 202021605586 U CN202021605586 U CN 202021605586U CN 213113292 U CN213113292 U CN 213113292U
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Abstract
The utility model belongs to the technical field of nucleic acid extraction, in particular to a nucleic acid extraction structure, which comprises a box body, a box cover and a box body groove, wherein a centrifugal tube hole is arranged in the box body groove and is used for placing a centrifugal tube filled with cracking liquid, washing liquid and eluent; the suction tube is movably accommodated in the suction tube hole, the suction tube mainly comprises a suction ball, a connecting part, a tube body, a gasket and a nucleic acid adsorption sheet, one end of the tube body is provided with a fixed groove, and the nucleic acid adsorption sheet is accommodated in the fixed groove; the utility model discloses with the absorption of nucleic acid, washing and elution function high integrated, blow and inhale the liquid through the suction tube and flow through the nucleic acid adsorption piece and reach nucleic acid adsorption, washing and elution purpose, centrifuging tube, reagent and the suction tube that use in the operation process are disposable, can effectively reduce pollution. The utility model discloses do not need other instrument and equipment supplementary, the quantity and the purity of drawing nucleic acid can satisfy the requirement that molecular biology detected commonly used, and conveniently carry, have solved basic unit and open-air sample nucleic acid extraction's a difficult problem.
Description
[ technical field ] A method for producing a semiconductor device
The utility model belongs to the technical field of nucleic acid extraction, concretely relates to nucleic acid extraction structure.
[ background of the invention ]
The molecular biological detection almost needs to extract sample nucleic acid, and the quality and efficiency of nucleic acid extraction determine the success or failure of detection results. Although the technical level is greatly improved, the nucleic acid extraction technology is in need of high-speed centrifugation and other equipment and repeated pipetting, centrifugation and other tedious operations, which are not beneficial to basic unit and field operation, and is the primary obstacle of popularization of molecular biology detection technology in basic level.
[ Utility model ] content
In order to achieve the above purpose, the utility model adopts the technical proposal that:
a nucleic acid extraction structure comprises a box body, a box cover and a box body groove, wherein one end of the box cover is hinged with the box body, and the opposite end of the box cover is connected with the box body in a buckling manner; a box body groove is arranged in the box body, and a lysis solution pipe hole, a washing solution pipe hole, an eluent pipe hole and a suction pipe hole are arranged in the box body groove; the suction pipe is movably contained in the suction pipe hole.
A plurality of centrifuge tube accommodating holes are formed in the lysis liquid tube hole, the washing liquid tube hole and the elution liquid tube hole; the centrifuge tube accommodating holes are annularly arrayed in the tube hole of the lysis solution, the tube hole of the washing solution and the tube hole of the elution solution;
a plurality of centrifuge tube accommodating holes in the lysis liquid tube hole are used for storing centrifuge tubes filled with lysis liquid, a plurality of centrifuge tube accommodating holes in the washing liquid tube hole are used for storing centrifuge tubes filled with washing liquid, and a plurality of centrifuge tube accommodating holes in the elution liquid tube hole are used for storing centrifuge tubes filled with eluent;
the suction tube comprises a suction ball, a connecting part, a tube body, a gasket and a nucleic acid adsorption sheet; the suction ball is fixedly connected with the connecting part, and the connecting part is detachably connected with the pipe body; the tube body is provided with a suction tube tip end part which is communicated with the tube body; one end of the tube body is also provided with a fixed groove, and the nucleic acid adsorption sheet is movably accommodated in the fixed groove; the connecting part is provided with an accommodating groove for accommodating the gasket.
Preferably, the end part of the suction pipe tip is of a hollow round table-shaped structure with two open ends.
Preferably, the nucleic acid adsorbing sheet in the pipette is one of a nucleic acid adsorbing silica gel membrane and a glass fiber membrane.
Compared with the prior art, the nucleic acid extraction structure of the utility model integrates the functions of nucleic acid adsorption, washing and elution to a high degree, and the purposes of nucleic acid adsorption, washing and elution are achieved by blowing and sucking liquid to flow through the nucleic acid adsorption sheet; the centrifuge tube, the related reagent and the suction tube which are involved in the operation process are disposable and can be replaced after being used; the quantity and purity of the extracted nucleic acid can meet the requirements of common molecular biology detection without the assistance of other instruments and equipment, the nucleic acid extraction efficiency is greatly increased, the carrying is convenient, and the problem of nucleic acid extraction of basic units and field samples is solved.
[ description of the drawings ]
FIG. 1 is a schematic structural view of the nucleic acid isolation structure of the present invention.
FIG. 2 is a schematic structural view of an aspiration tube of the nucleic acid isolation structure of the present invention.
FIG. 3 is a schematic view of the structure of the tube body of the nucleic acid extracting structure of the present invention.
FIG. 4 is a schematic structural view of the connecting portion and the pipette ball of the nucleic acid isolation structure of the present invention.
Description of the main elements
| Case 1 | |
|
| Lysis |
Cleaning |
|
| |
Centrifuge |
Suction pipe 3 |
| |
Connecting |
|
| |
Nucleic |
|
| |
|
Centrifuge tube 116 |
The following detailed description of the invention will be further described in conjunction with the above-identified drawings.
[ detailed description ] embodiments
Clearly, and completely, it is to be understood that the described embodiments are merely illustrative of some, but not all, of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
It should be noted that all the directional indicators (such as upper, lower, left, right, front and rear … …) in the embodiment of the present invention are only used to explain the relative position relationship between the components, the motion situation, etc. in a specific posture (as shown in the drawings), and if the specific posture is changed, the directional indicator is changed accordingly.
In addition, the descriptions related to "first", "second", etc. in the present invention are for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicit ly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, the meaning of "and/or" appearing throughout is to include three juxtapositions, exemplified by "A and/or B," including either the A or B arrangement, or both A and B satisfied arrangement. In addition, the technical solutions in the embodiments may be combined with each other, but it must be based on the realization of those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should not be considered to exist, and is not within the protection scope of the present invention.
Referring to fig. 1-4, a nucleic acid extraction structure comprises a case body 1, a case cover 2 and a case body groove 11, wherein one end of the case cover 2 is hinged with the case body 1, and the opposite end of the case cover 2 is connected with the case body 1 in a buckling manner; a box body groove 11 is arranged in the box body 1, and a lysis solution pipe hole 111, a washing solution pipe hole 112, an eluent pipe hole 113 and a plurality of suction pipe holes 114 are arranged in the box body groove 11; each suction pipe 3 is movably accommodated in the corresponding suction pipe hole 114;
in this embodiment, be provided with the box on the box 1, be provided with on case lid 2 with box complex pin thread, realize through the box with the pin thread cooperation that case lid 2 is relative one end and box 1 buckle is connected.
A plurality of centrifuge tube accommodating holes 115 are formed in the lysis solution tube hole 111, the washing solution tube hole 112 and the eluent tube hole 113; the centrifuge tube accommodating holes 115 are arranged in the lysis solution tube hole 111, the washing solution tube hole 112 and the eluent tube hole 113 in an annular array;
a plurality of centrifuge tube accommodating holes 115 in the lysis solution tube hole 111 are used for storing centrifuge tubes 116 filled with lysis solution, a plurality of centrifuge tube accommodating holes 115 in the washing solution tube hole 112 are used for storing centrifuge tubes 116 filled with washing solution, and a plurality of centrifuge tube accommodating holes 115 in the eluent tube hole 113 are used for storing centrifuge tubes 116 filled with eluent;
the suction tube 3 comprises a suction ball 31, a connecting part 32, a tube body 33, a gasket 34 and a nucleic acid adsorption sheet 35; the suction ball 31 is fixedly connected with the connecting part 32, and the connecting part 32 is detachably connected with the pipe body 33; in the present embodiment, the connecting portion 32 is provided with an internal thread, and the tube body 33 is provided with an external thread; the connecting part 32 is matched with the pipe body 33 through internal threads and external threads, and the connecting part 32 is detachably connected with the pipe body 33; the tube body 33 is provided with an aspiration tube tip portion 331, the aspiration tube tip portion 331 communicating with the tube body 33; in this embodiment, the suction tip 331 has a hollow truncated cone-shaped structure with two open ends. A fixing groove 332 is further provided at one end of the tube 33, and the fixing groove 332 is used for accommodating the nucleic acid adsorbing sheet 35; the connecting portion 32 is provided with a housing groove 321, and the housing groove 321 is used for housing the gasket 34. The nucleic acid adsorbing sheet 35 is movably accommodated in the fixing groove 332.
In the present embodiment, the suction ball 31, the connection portion 32, the tube body 33, and the suction tube tip portion 331 communicate with each other.
In the present embodiment, the gasket 34 has a ring structure.
When the nucleic acid extraction structure is used, the box cover 2 is opened, the suction tube 3 is taken out from the suction tube hole 114 in the box body groove 11, the suction ball 31 is pinched by the suction tube 3, and a sample of nucleic acid to be extracted is sucked into the tube body 33; inserting the tip 331 of the suction tube into one of the centrifuge tubes 116 storing lysis solution in the tube holes 111 of lysis solution, releasing the sample in the tube body 33 into the centrifuge tube 116 storing lysis solution, so that the lysis solution in the centrifuge tube 116 storing lysis solution cracks the sample, using the suction tube 3 to blow, suck or shake the sample uniformly, standing the sample for 15 to 30 minutes, then using the suction tube 3 to suck the sample and the lysis solution in the centrifuge tube 116 storing lysis solution into the connecting part 32 through the tube body 33, so that the liquid infiltrates the nucleic acid adsorption sheet 35; then the suction ball 31 is pressed to blow out the liquid, the blowing and the suction are repeated for 3 to 5 times, and then all the liquid is blown out; then the suction tube 3 is transferred and put into a centrifuge tube 116 filled with a washing solution for washing, the washing solution is completely sucked into the connecting part 32 through the tube body 33 and the nucleic acid adsorption sheet 35 by pressing and releasing the suction ball 31, then the suction ball 31 is pressed for blowing out, and the blowing-in and the sucking are repeated for 3-5 times, and then all the liquid is blown out; then, the centrifugal tube 116 filled with the cleaning solution is replaced to carry out repeated washing, all liquid is blown out after the washing is finished, and the liquid is naturally dried for 3-5 min; and transferring the absorption tube 3 into a centrifuge tube 116 filled with eluent for elution, repeatedly blowing and absorbing for 3-5 times, fully washing the nucleic acid adsorption sheet 35 by the eluent each time, releasing the nucleic acid in the nucleic acid adsorption sheet 35 into the eluent, and blowing all the eluent in the absorption tube 3 into a new empty centrifuge tube after the operation is finished, wherein the eluent in the centrifuge tube contains the extracted nucleic acid.
In the operation process, except for the preservation of the centrifugal tube filled with the eluent in the last step, the centrifugal tube, the reagent and the absorption tube 3 used in the operation process are all replaced, and then the nucleic acid extraction of the next sample is carried out.
In this embodiment, the centrifuge tube 116, the corresponding reagent and the pipette 3 are all disposable and are replaced after use.
In this embodiment, the nucleic acid-adsorbing sheet 35 is a nucleic acid-adsorbing silica gel membrane or a glass fiber membrane.
In this embodiment, the nucleic acid-adsorbing sheet 35 is a silica membrane manufactured by Hangzhou maple Biotechnology Co., Ltd or a glass fiber membrane manufactured by Youbite laboratory instruments development Co., Ltd.
The above description is for the detailed description of the preferred possible embodiments of the present invention, but the embodiments are not intended to limit the scope of the present invention, and all equivalent changes or modifications accomplished under the technical spirit suggested by the present invention should fall within the scope of the present invention.
Claims (4)
1. A nucleic acid extraction structure is characterized by comprising a box body, a box cover and a box body groove, wherein one end of the box cover is hinged with the box body, and the opposite end of the box cover is connected with the box body in a buckling manner; a box body groove is arranged in the box body, and a lysis solution pipe hole, a washing solution pipe hole, an eluent pipe hole and a suction pipe hole are arranged in the box body groove; the suction pipe is movably accommodated in the suction pipe hole;
a plurality of centrifuge tube accommodating holes are formed in the lysis liquid tube hole, the washing liquid tube hole and the elution liquid tube hole; the centrifuge tube accommodating holes are annularly arrayed in the tube hole of the lysis solution, the tube hole of the washing solution and the tube hole of the elution solution;
a plurality of centrifuge tube accommodating holes in the lysis liquid tube hole are used for storing centrifuge tubes filled with lysis liquid, a plurality of centrifuge tube accommodating holes in the washing liquid tube hole are used for storing centrifuge tubes filled with washing liquid, and a plurality of centrifuge tube accommodating holes in the elution liquid tube hole are used for storing centrifuge tubes filled with eluent;
the suction tube comprises a suction ball, a connecting part, a tube body, a gasket and a nucleic acid adsorption sheet; the suction ball is fixedly connected with the connecting part, and the connecting part is detachably connected with the pipe body; the tube body is provided with a suction tube tip end part which is communicated with the tube body; one end of the tube body is also provided with a fixed groove, and the nucleic acid adsorption sheet is movably accommodated in the fixed groove; the connecting part is provided with an accommodating groove for accommodating the gasket.
2. The nucleic acid isolation structure according to claim 1, wherein the tip of the aspiration tube has a hollow truncated cone-like structure with both ends open.
3. The nucleic acid extraction structure of claim 1, wherein the nucleic acid adsorption sheet in the aspiration tube is one of a nucleic acid adsorption silica gel membrane or a glass fiber membrane.
4. The nucleic acid isolation structure according to claim 1, wherein the gasket in the aspiration tube has a ring-like structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021605586.1U CN213113292U (en) | 2020-08-05 | 2020-08-05 | Nucleic acid extraction structure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021605586.1U CN213113292U (en) | 2020-08-05 | 2020-08-05 | Nucleic acid extraction structure |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213113292U true CN213113292U (en) | 2021-05-04 |
Family
ID=75682870
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021605586.1U Active CN213113292U (en) | 2020-08-05 | 2020-08-05 | Nucleic acid extraction structure |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213113292U (en) |
-
2020
- 2020-08-05 CN CN202021605586.1U patent/CN213113292U/en active Active
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