CN212307906U - Exhaust type liquid quick collecting device - Google Patents
Exhaust type liquid quick collecting device Download PDFInfo
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- CN212307906U CN212307906U CN202021600680.8U CN202021600680U CN212307906U CN 212307906 U CN212307906 U CN 212307906U CN 202021600680 U CN202021600680 U CN 202021600680U CN 212307906 U CN212307906 U CN 212307906U
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Abstract
The utility model provides a quick collection device of exhaust formula liquid, the device includes at least: the liquid collecting assembly is at least partially nested in an inner cavity of the negative pressure connecting sleeve and comprises a filtering pipe, a negative pressure exhaust pipe and a liquid collecting pipe which are sequentially communicated from top to bottom, the filtering pipe is provided with an external communicating port for communicating with the outside, and a filtering pipe cover is arranged on the external communicating port; the side wall of the negative pressure exhaust pipe is provided with an exhaust small hole, and the liquid collecting assembly is communicated with the negative pressure connecting sleeve through the exhaust small hole; and the negative pressure connecting sleeve is provided with a negative pressure connecting hole. The utility model solves the problem of rapid sample treatment in field detection, so that nucleic acid extraction is completed within 5min, and the detection efficiency is greatly improved; in addition, a specialized large-scale nucleic acid extractor is not needed, and the equipment is extremely simple to operate.
Description
Technical Field
The utility model relates to a biological detection apparatus field especially relates to a quick collection device of exhaust formula liquid.
Background
The gene detection has been widely applied to various scenes such as clinical examination, disease prevention and control, food safety detection, import and export quarantine detection and the like due to the characteristics of strong detection specificity, high sensitivity, short window period and the like. The requirements for the prevention and control of infectious diseases and the rapid and accurate gene diagnosis are more outstanding. In recent times, the magnetic bead method nucleic acid extraction technology is widely used due to the advantages of good nucleic acid recovery specificity, high purity, convenience for automation and the like, but in the application environments with high timeliness such as the first line of emergency, the first-aid site and the like, the magnetic bead method nucleic acid extraction technology is not convenient to apply due to the defects of long time consumption, requirement of specialized equipment, immobility and the like.
SUMMERY OF THE UTILITY MODEL
In view of the above-mentioned shortcomings of the prior art, it is an object of the present invention to provide a rapid collection device for exhaust liquid. The small-sized equipment which is simple and convenient to operate, does not need specialized large-sized equipment, can be moved for operation and consumes little time is provided, the efficiency of field detection can be greatly improved, and the workload of an inspection technician is reduced.
To achieve the above and other related objects, the present invention provides an exhaust type liquid rapid collecting apparatus, which at least comprises: the liquid collecting assembly is at least partially nested in an inner cavity of the negative pressure connecting sleeve, the liquid collecting assembly comprises a filtering pipe, a negative pressure exhaust pipe and a liquid collecting pipe which are sequentially communicated from top to bottom, the filtering pipe is provided with an external communicating port used for being communicated with the outside, a filtering pipe cover is arranged on the external communicating port, and a filtering piece is arranged in a cavity of the filtering pipe; the side wall of the negative pressure exhaust pipe is provided with an exhaust small hole, and the liquid collecting assembly is communicated with the negative pressure connecting sleeve through the exhaust small hole; the negative pressure connecting sleeve is provided with a negative pressure connecting hole, and when the filter tube cover is closed, the exhaust type liquid rapid collecting device is communicated with the outside through the negative pressure connecting hole.
As mentioned above, the utility model discloses a quick collection device of exhaust formula liquid has following beneficial effect:
the utility model solves the problem of rapid sample treatment in field detection, so that nucleic acid extraction is completed within 5min, and the detection efficiency is greatly improved; in addition, a specialized large-scale nucleic acid extractor is not needed, the transportation is convenient, and the rapid field detection and the vehicle-mounted detection are possible; the device is extremely simple to operate. The utility model discloses a core mainly is to utilize the pressure differential, at liquid vaporization and discharge process, because the change of instantaneous atmospheric pressure, most liquid still is in the liquid state, under the dual influence of pulling force and gravity down, liquid is collected in the below liquid collector of device. The utility model discloses if supporting filter collector and nucleic acid extraction reagent, then can ultrafast handle the sample and draw and obtain nucleic acid, when obtaining high-purity nucleic acid, efficiency is showing and is improving.
Drawings
Fig. 1 is a perspective view of a rapid liquid collecting apparatus with air discharge according to an embodiment of the present invention.
Fig. 2 is a structural diagram of an exhaust type liquid rapid collecting device according to an embodiment of the present invention.
Fig. 3 is a perspective view of a rapid liquid collection device with air discharge according to another embodiment of the present invention.
Fig. 4 is a structural view of a rapid collecting device for liquid discharged from a discharge port according to another embodiment of the present invention.
Fig. 5 is a view showing a liquid collecting module of a rapid collecting device of an exhaust type according to another embodiment of the present invention.
Fig. 6 is a perspective view of a liquid collecting module of a rapid collecting device for liquid discharged from a gas discharge apparatus according to another embodiment of the present invention.
Description of the element reference numerals
1 negative pressure connecting sleeve
11 negative pressure connecting hole
2 liquid collecting assembly
21 filtering pipe
211 filter tube cover
212 negative pressure exhaust pipe communicating opening
213 pipe diameter contraction section
22 negative pressure exhaust pipe
221 air exhaust small hole
23 liquid collecting pipe
Detailed Description
The following description is provided for illustrative purposes, and other advantages and features of the present invention will become apparent to those skilled in the art from the following detailed description.
Please refer to fig. 1-6. It should be understood that the structure, ratio, size and the like shown in the drawings attached to the present specification are only used for matching with the content disclosed in the specification, so as to be known and read by those skilled in the art, and are not used for limiting the limit conditions that the present invention can be implemented, so that the present invention has no technical essential meaning, and any structure modification, ratio relationship change or size adjustment should still fall within the scope that the technical content disclosed in the present invention can cover without affecting the function that the present invention can produce and the purpose that the present invention can achieve. Meanwhile, the terms such as "upper", "lower", "left", "right", "middle" and "one" used in the present specification are for convenience of description, and are not intended to limit the scope of the present invention, and changes or adjustments of the relative relationship thereof may be made without substantial technical changes, and the present invention is also regarded as the scope of the present invention.
As shown in fig. 1 and 3, the exhaust type liquid rapid collecting apparatus of the present embodiment includes at least: the negative pressure connecting sleeve comprises a negative pressure connecting sleeve 1 and a liquid collecting assembly 2, wherein at least part of the liquid collecting assembly 2 is nested in an inner cavity of the negative pressure connecting sleeve 1, the liquid collecting assembly 2 comprises a filtering pipe 21, a negative pressure exhaust pipe 22 and a liquid collecting pipe 23 which are sequentially communicated from top to bottom, the filtering pipe 21 is provided with an external communicating port for communicating with the outside, a filtering pipe cover 211 is arranged on the external communicating port, and a filtering element is arranged in a cavity of the filtering pipe; as shown in fig. 1 and 5, the side wall of the negative pressure exhaust pipe 22 is provided with an exhaust small hole 221, and the liquid collecting assembly 2 is communicated with the negative pressure connecting sleeve 1 through the exhaust small hole 221; the negative pressure connecting sleeve 1 is provided with a negative pressure connecting hole 11, and when the filter tube cover 211 is covered, the exhaust type liquid quick collecting device is communicated with the outside through the negative pressure connecting hole 11.
The negative pressure connecting sleeve is required to be guaranteed to form a sealed environment at least with the filter pipe and the negative pressure exhaust pipe, and a negative pressure suction filtration environment is formed in a matched mode, so that the filtering treatment of a sample and the quick collection of liquid are accelerated.
Further, as shown in fig. 1 and 3, at least the lower part of the filtering pipe 21 is nested in the negative pressure exhaust pipe 22, a negative pressure exhaust pipe communication port 212 is provided at the lower end of the filtering pipe 21, and the negative pressure exhaust pipe communication port 212 is communicated with the negative pressure exhaust pipe 22. The filter tube can play a role in rinsing impurities by adding different buffer solutions while filtering and enriching nucleic acid, and finally, nucleic acid is eluted by adding eluent.
Preferably, as shown in fig. 1 and 3, the lower portion of the filtering pipe 21 includes a pipe diameter contraction section 213, and a side wall of the pipe diameter contraction section 213 contracts toward the inside of the filtering pipe from top to bottom; the lower end of the pipe diameter contraction section 213 is communicated with the negative pressure exhaust pipe communication port 213. Compared with a flat-bottom filter, the sharp-bottom shrinkage filter has the sharp-bottom function, and the liquid compression is intensified by the negative pressure device, so that the liquid is easier to liquefy and drip in the form of water drops.
The filter element comprises a filter membrane. The aperture of the filter membrane can be 0.1-5 microns. Used for filtering impurities and adsorbing nucleic acid.
The adsorption and release of nucleic acids is achieved by a change in the concentration of the liquid, typically high salt adsorption, low salt elution. After filtering impurities, a low-salt reagent (eluent) can be added to elute nucleic acid, and the nucleic acid is washed into a liquid collecting pipe.
The filter tube is preferably a disposable filter. Preventing cross contamination. The common sharp-bottomed contracted filter tube on the market can be adopted. Such as Shanghai river organism ME-0010 or Tiangen DP 315.
Optionally, the aperture of the small exhaust hole 221 is 0.1mm to 20 mm. The smaller the pore size, the greater the number of said small vent holes 221 can be. Under the action of negative pressure, a negative pressure state is quickly formed in the device, liquid on the filter element is quickly liquefied into a water drop shape and is dripped into the liquid collecting pipe, and partially vaporized liquid is discharged from the small exhaust hole.
The exhaust small holes can be flexibly arranged on the side wall of the negative pressure exhaust pipe 22, and can be arranged on the upper part of the side wall of the negative pressure exhaust pipe 22 and close to the filter pipe 21; or may be provided at a lower portion of the side wall of the negative pressure gas discharge pipe 22 near the liquid collection pipe.
The negative pressure exhaust pipe is preferably a disposable product, and can further ensure that the liquid in the liquid collecting pipe does not have the risk of cross contamination.
Preferably, as shown in fig. 2 and 6, the liquid collection pipe 23 is detachably connected to the negative pressure exhaust pipe 22.
In one embodiment, the liquid collection tube 23 is threaded to the negative pressure exhaust tube 22.
The volume of the liquid collected in the liquid collecting pipe can be 50-500 microliters according to the difference of the downstream usage amount.
In one embodiment, as shown in fig. 3, 4, 5 and 6, the liquid collection assembly 2 body is nested in the inner cavity of the negative pressure connection sleeve 1, and the filter tube cover 211 is exposed to the outside of the negative pressure connection sleeve 1. At the moment, the negative pressure connecting sleeve, the filter pipe, the negative pressure exhaust pipe and the liquid collecting pipe form a sealed environment. The whole exhaust type liquid quick collecting device can be a disposable product.
As shown in fig. 1 and 2, the liquid collection tube 23 is exposed to the outside of the negative pressure connection sleeve 1. At the moment, the negative pressure connecting sleeve, the filter pipe and the negative pressure exhaust pipe form a sealed environment; the negative pressure connecting sleeve can be recycled, a negative pressure state is quickly formed in the sleeve under the action of negative pressure through the separation of the circulating type negative pressure connecting sleeve and the liquid collecting pipe, liquid on the filtering membrane is quickly liquefied into a water droplet shape and is dripped into the liquid collecting pipe, part of vaporized liquid is discharged from the small exhaust holes and does not pass through the outer wall of the liquid collecting pipe, and formed vaporized aerosol cannot pass through the liquid collecting pipe, namely cannot be adhered to the pipe wall of the liquid collecting pipe to cause pollution to the pipe wall.
When the liquid collecting device is used, the liquid collecting pipe is screwed and fixed below the negative pressure exhaust pipe, and the filter pipe is nested in the negative pressure exhaust pipe to form the liquid collecting assembly; then nesting the liquid collecting assembly in a negative pressure connecting sleeve, and connecting the liquid collecting assembly with a vacuum pump through a negative pressure connecting hole; pouring a sample to be treated into the filter pipe, starting the vacuum pump to form negative pressure in the device, and collecting liquid into the liquid collecting pipe; after the liquid is collected, the liquid collecting pipe can be suspended and then covered with a cover for sealing and storing so as to be used for downstream detection.
The exhaust type liquid rapid collecting device can be used in the field of nucleic acid extraction. Can be used for extracting samples such as swab samples, serum samples, plasma samples, excrement samples and the like.
Example 1 Rapid nucleic acid extraction of swab samples
The existing method usually uses manual, semi-automatic or automatic equipment to add the sample washed from the swab to the instrument for nucleic acid extraction, and the ultra-fast sample treatment is difficult to achieve. This example allows for rapid nucleic acid extraction by direct addition of a swab wash sample.
A. Collecting a sample by using a swab, and adding the sample into a collecting pipe with a sample preservation solution;
B. adding 600 microliters of the sample into a filter of the exhaust type liquid rapid collection device, and starting a negative pressure device to suck for 1 min;
C. sequentially and continuously adding 600ul of washing solution 1 and washing solution 2, and pumping for 15s respectively;
D. sucking for 1 min;
E. adding 100 microliter eluent, and pumping for 10s to obtain the desired nucleic acid product.
EXAMPLE 2 Rapid nucleic acid extraction of serum/plasma samples
This example allows for rapid nucleic acid extraction by direct addition of serum/plasma samples.
A. Adding 200 microliters of sample into a filter of the exhaust type liquid rapid collection device, adding 500 microliters of lysate, and incubating for 1 min;
B. starting a negative pressure device to suck for 1 min;
C. sequentially and continuously adding 500ul of washing solution 1 and washing solution 2, and pumping for 15s respectively;
D. sucking for 1 min;
E. adding 100 microliter eluent, and pumping for 10s to obtain the desired nucleic acid product.
Example 3 Rapid nucleic acid extraction of stool samples
This example allows for rapid nucleic acid extraction by direct addition of a stool wash sample.
A. Collecting a fecal sample and adding the fecal sample into a collecting pipe with sample preservation solution;
B. adding 600 microliters of the sample into a filter of the exhaust type liquid rapid collection device, and starting a negative pressure device to suck for 1 min;
C. sequentially and continuously adding 600ul of washing solution 1 and washing solution 2, and pumping for 15s respectively;
D. sucking for 1 min;
E. adding 100 microliter eluent, and pumping for 10s to obtain the desired nucleic acid product.
The above embodiments are merely illustrative of the principles and effects of the present invention, and are not to be construed as limiting the invention. Modifications and variations can be made to the above-described embodiments by those skilled in the art without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which may be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.
Claims (9)
1. A rapid collection device of the vented liquid, characterized in that it comprises at least: the liquid collecting device comprises a negative pressure connecting sleeve (1) and a liquid collecting assembly (2), wherein at least part of the liquid collecting assembly (2) is nested in an inner cavity of the negative pressure connecting sleeve (1), the liquid collecting assembly (2) comprises a filtering pipe (21), a negative pressure exhaust pipe (22) and a liquid collecting pipe (23) which are sequentially communicated from top to bottom, the filtering pipe (21) is provided with an external communicating port for communicating with the outside, a filtering pipe cover (211) is arranged on the external communicating port, and a filtering element is arranged in a cavity of the filtering pipe; the side wall of the negative pressure exhaust pipe (22) is provided with an exhaust small hole (221), and the liquid collecting assembly (2) is communicated with the negative pressure connecting sleeve (1) through the exhaust small hole (221); the negative pressure connecting sleeve (1) is provided with a negative pressure connecting hole (11), and when the filter tube cover (211) is covered, the exhaust type liquid quick collecting device is communicated with the outside through the negative pressure connecting hole (11).
2. The vented liquid rapid collecting device according to claim 1, wherein at least the lower part of the filtering tube (21) is nested in the negative pressure vent pipe (22), and the lower end of the filtering tube (21) is provided with a negative pressure vent pipe communicating port (212) which is communicated with the negative pressure vent pipe (22).
3. The vented liquid rapid collecting device according to claim 2, wherein the lower part of the filtering tube (21) comprises a tube diameter constriction section (213), the side wall of the tube diameter constriction section (213) is constricted from top to bottom to the inside of the filtering tube; the lower end of the pipe diameter contraction section (213) is communicated with the negative pressure exhaust pipe communication port (212).
4. The vented liquid rapid collection device of claim 1, wherein the filter comprises a filter membrane.
5. The vented liquid rapid collector according to claim 1, wherein the vent small hole (221) has a hole diameter of 0.1mm to 20 mm.
6. The vented liquid rapid-collecting apparatus according to claim 1, wherein the liquid collecting pipe (23) is detachably connected to the negative pressure exhaust pipe (22).
7. The vented liquid rapid-collecting device according to claim 6, wherein the liquid collecting pipe (23) is screwed with the negative pressure exhaust pipe (22).
8. The vented liquid rapid collection device according to claim 1, wherein the liquid collection module (2) body is nested in the inner cavity of the negative pressure connection sleeve (1), and the filter tube cover (211) is exposed to the outside of the negative pressure connection sleeve (1).
9. The vented liquid rapid collecting device according to claim 1, wherein the liquid collecting tube (23) is exposed to the outside of the negative pressure connection sleeve (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021600680.8U CN212307906U (en) | 2020-08-05 | 2020-08-05 | Exhaust type liquid quick collecting device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021600680.8U CN212307906U (en) | 2020-08-05 | 2020-08-05 | Exhaust type liquid quick collecting device |
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| Publication Number | Publication Date |
|---|---|
| CN212307906U true CN212307906U (en) | 2021-01-08 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202021600680.8U Active CN212307906U (en) | 2020-08-05 | 2020-08-05 | Exhaust type liquid quick collecting device |
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| CN (1) | CN212307906U (en) |
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