CN110257372B - Nucleic acid extraction instrument - Google Patents
Nucleic acid extraction instrument Download PDFInfo
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- CN110257372B CN110257372B CN201910696199.9A CN201910696199A CN110257372B CN 110257372 B CN110257372 B CN 110257372B CN 201910696199 A CN201910696199 A CN 201910696199A CN 110257372 B CN110257372 B CN 110257372B
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- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 51
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 51
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 51
- 238000000605 extraction Methods 0.000 title claims description 16
- 230000007246 mechanism Effects 0.000 claims abstract description 22
- 230000005540 biological transmission Effects 0.000 claims description 18
- 238000007789 sealing Methods 0.000 claims description 16
- 238000004887 air purification Methods 0.000 claims description 8
- 244000309464 bull Species 0.000 claims description 2
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 11
- 238000003915 air pollution Methods 0.000 abstract description 5
- 230000008901 benefit Effects 0.000 abstract description 4
- 238000005086 pumping Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 description 5
- 238000000746 purification Methods 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Extraction Or Liquid Replacement (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a nucleic acid extractor, which comprises an extractor body, wherein the left side of the extractor body is communicated with an air inlet pipe, the inside of the air inlet pipe is fixedly connected with a fixing sleeve through a connecting plate, and the right side of the extractor body is communicated with a positioning pipe. According to the invention, the air in the extractor body is pumped by the fan through the rotating sleeve and the positioning pipe, so that the external air enters the extractor body through the air inlet pipe and the air inlet groove, and the pumped air is purified by the air purifying device, so that the advantage of pumping and purifying the volatilized air is achieved, the problem that the existing nucleic acid extractor is not provided with a certain air treatment mechanism, when the nucleic acid extractor is opened, the air volatilized by the reagent can be discharged to cause air pollution, and part of the gas left in the nucleic acid extractor is easy to pollute the reagent for extracting nucleic acid next time is solved, and the practicability of the nucleic acid extractor is improved.
Description
Technical Field
The invention relates to the technical field of extractors, in particular to a nucleic acid extractor.
Background
The nucleic acid extraction instrument is an instrument for automatically completing sample nucleic acid extraction by using matched nucleic acid extraction reagents, and is widely applied to various fields such as disease control centers, clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microorganism detection, food safety detection, animal husbandry, molecular biology research and the like.
Nucleic acid is a biological macromolecular compound polymerized by a plurality of polynucleotides and is one of the most basic substances of life, and a nucleic acid extractor is usually used for extracting in the process of extracting nucleic acid, but the existing nucleic acid extractor does not have a certain gas treatment mechanism, when the nucleic acid extractor is opened, gas volatilized by a reagent can be discharged to cause air pollution, and part of gas left in the nucleic acid extractor is easy to pollute the reagent for extracting nucleic acid next time, so that the practicability of the nucleic acid extractor is reduced.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a nucleic acid extractor, which has the advantage of extracting and purifying volatilized gas, and solves the problems that the existing nucleic acid extractor is not provided with a certain gas treatment mechanism, when the nucleic acid extractor is opened, gas volatilized by a reagent can be discharged to cause air pollution, and part of gas left in the nucleic acid extractor is easy to pollute the reagent for extracting nucleic acid next time.
In order to achieve the above purpose, the present invention provides the following technical solutions: the utility model provides a nucleic acid extraction appearance, includes draws the appearance body, the left side intercommunication of drawing the appearance body has the intake pipe, the inside of intake pipe is through the fixed cover of connecting plate fixedly connected with, the right side intercommunication of drawing the appearance body has the locating tube, the top fixed mounting who draws appearance body right side has air purification device, the right side fixedly connected with who draws the appearance body is located the fan at locating tube top, the output of fan passes through the left side of connecting pipe intercommunication in air purification device bottom, the inside of locating tube transversely is provided with the connecting rod, the left side fixedly connected with seal cover on connecting rod surface, the surface of seal cover contacts with the inner wall of locating tube, the right side threaded connection of locating tube inner chamber has the driving sleeve, the right side on driving sleeve surface has cup jointed the rotation cover, the input of fan is on the right side of rotation cover through bellows intercommunication. The positioning tube communicates with a nucleic acid extraction chamber of a conventional nucleic acid extractor.
Preferably, sealing mechanism is provided with in the inside of intake pipe, sealing mechanism includes sleeve, backing plate, air inlet tank, rubber sleeve one and extension spring, sleeve sliding connection is in the inside of fixed cover, backing plate fixed connection is on telescopic right side, the air inlet tank is seted up on the right side of intake pipe inner wall top and bottom, extension spring fixed connection is on the right side of sleeve inner wall, the left end fixed connection of extension spring is in the left side of fixed cover inner wall, rubber sleeve one fixed connection is in the left side of backing plate, the left side of rubber sleeve one and the inner wall contact of intake pipe. The air inlet tank is communicated with a nucleic acid extraction chamber of a conventional nucleic acid extractor.
Preferably, the surface fixedly connected with slider of connecting rod, the spout has been seted up to the inside of locating tube, slider sliding connection is in the inside of spout.
Preferably, the left end fixedly connected with baffle of connecting rod, the right side fixedly connected with of baffle is in the left side of seal cover.
Preferably, the right side of rotating the cover inner wall fixedly connected with rubber cover two, the left side of rubber cover two contacts with the right side of transmission cover, the top and the bottom of transmission cover all fixedly connected with be located the bull stick in the registration arm outside.
Preferably, the right-hand member of connecting rod is provided with coupling mechanism, coupling mechanism includes spacing dish, stop collar and gag lever post, spacing dish fixed connection is at the right-hand member of connecting rod, the stop collar cup joints the surface at the spacing dish, gag lever post fixed connection is on the surface of stop collar, the one end fixed connection that the stop collar was kept away from to the gag lever post is in the inside of drive sleeve, the shape on drive sleeve surface and the shape on sleeve surface are T shape.
Compared with the prior art, the invention provides a nucleic acid extractor, which has the following beneficial effects:
1. this nucleic acid extractor, by the fan through rotating cover and positioning tube to the inside air of extractor body and drawing, make outside air get into the inside of extractor body through intake pipe and air inlet tank, carry out purification treatment by air purification device to the gas of taking out again, thereby possess the advantage that can take out the purification to volatilized gas, it does not possess certain gas processing mechanism to have solved current nucleic acid extractor, when opening the nucleic acid extractor, the gaseous just can release that the reagent volatilized causes air pollution, and leave the partial gas in the nucleic acid extractor then easily causes the problem of pollution to the reagent of next extraction nucleic acid, the practicality of nucleic acid extractor has been improved.
2. This nucleic acid extractor through setting up the extension spring, can carry out the pulling of certain degree to the backing plate through the elasticity, makes the backing plate seal through a pair of intake pipe of rubber sleeve, has improved the stability of backing plate, through setting up slider and spout, can spacing the connecting rod, has avoided the connecting rod to appear pivoted phenomenon when removing, has improved the stability when the connecting rod removes, through setting up rubber sleeve two, can seal the gap between rotating sleeve and the driving sleeve, has avoided the phenomenon that gas waft from the gap easily, has improved the leakproofness of driving sleeve, through all setting up the shape on driving sleeve surface and the shape on sleeve surface to T shape, can spacing the sleeve, avoided the sleeve to appear breaking away from fixed cover's phenomenon when using, has improved the stability of sleeve when using.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is an enlarged schematic view of FIG. 1 at A;
fig. 3 is an enlarged schematic view at B in fig. 1.
In the figure: the extraction instrument comprises an extraction instrument body 1, an air inlet pipe 2, a fixed sleeve 3, a positioning pipe 4, an air purifying device 5, a fan 6, a connecting rod 7, a sealing sleeve 8, a transmission sleeve 9, a rotating sleeve 10, a sealing mechanism 11, a sleeve 111, a backing plate 112, an air inlet groove 113, a rubber sleeve 114I, a tension spring 115, a sliding block 12, a sliding groove 13, a baffle 14, a rubber sleeve 15 II, a rotating rod 16, a connecting mechanism 17, a limiting disc 171, a limiting sleeve 172 and a limiting rod 173.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to FIGS. 1-3, a nucleic acid extractor comprises an extractor body 1, an air inlet pipe 2 is communicated with the left side of the extractor body 1, a fixing sleeve 3 is fixedly connected to the inside of the air inlet pipe 2 through a connecting plate, a positioning pipe 4 is communicated with the right side of the extractor body 1, the positioning pipe 4 is communicated with a nucleic acid extracting chamber of the nucleic acid extractor 1, an air purifying device 5 is fixedly arranged at the top of the right side of the extractor body 1, a sealing mechanism 11 is arranged in the air inlet pipe 2, the sealing mechanism 11 comprises a sleeve 111, a base plate 112, an air inlet groove 113, a rubber sleeve 114 and a tension spring 115, the sleeve 111 is slidably connected in the fixing sleeve 3, the base plate 112 is fixedly connected to the right side of the sleeve 111, the air inlet groove 113 is arranged on the right side of the top and the bottom of the inner wall of the air inlet pipe 2, the air inlet groove 113 is communicated with the nucleic acid extracting chamber of the nucleic acid extractor 1, the tension spring 115 is fixedly connected to the right side of the inner wall of the sleeve 111, the left end of the tension spring 115 is fixedly connected to the left side of the inner wall of the fixed sleeve 3, the first rubber sleeve 114 is fixedly connected to the left side of the base plate 112, the left side of the first rubber sleeve 114 is contacted with the inner wall of the air inlet pipe 2, the base plate 112 can be pulled to a certain extent through elasticity by arranging the tension spring 115, the base plate 112 can seal the air inlet pipe 2 through the first rubber sleeve 114, the stability of the base plate 112 is improved, the right side of the extractor body 1 is fixedly connected with the fan 6 positioned at the top of the positioning pipe 4, the output end of the fan 6 is communicated to the left side at the bottom of the air purifying device 5 through a connecting pipe, the inside of the positioning pipe 4 is transversely provided with the connecting rod 7, the surface of the connecting rod 7 is fixedly connected with the sliding block 12, the sliding groove 13 is arranged in the inside of the positioning pipe 4, the sliding block 12 is in the sliding groove 13, the connecting rod 7 can be limited through arranging the sliding block 12 and the sliding groove 13, the phenomenon that the connecting rod 7 rotates during moving is avoided, the stability of the connecting rod 7 during moving is improved, the sealing sleeve 8 is fixedly connected to the left side of the surface of the connecting rod 7, the baffle 14 is fixedly connected to the left side of the sealing sleeve 8, the right side of the baffle 14 is fixedly connected to the left side of the sealing sleeve 8, the surface of the sealing sleeve 8 is contacted with the inner wall of the positioning tube 4, the right side of the inner cavity of the positioning tube 4 is in threaded connection with the transmission sleeve 9, the right side of the surface of the transmission sleeve 9 is sleeved with the rotating sleeve 10, the right side of the inner wall of the rotating sleeve 10 is fixedly connected with the rubber sleeve II 15, the left side of the rubber sleeve II 15 is contacted with the right side of the transmission sleeve 9, the top and the bottom of the transmission sleeve 9 are fixedly connected with the rotating rod 16 positioned on the outer side of the positioning tube 4, the gap between the rotating sleeve 10 and the transmission sleeve 9 can be sealed by arranging the rubber sleeve II 15, and the phenomenon that gas easily flows out from the gap is avoided, the tightness of the transmission sleeve 9 is improved, the right end of the connecting rod 7 is provided with a connecting mechanism 17, the connecting mechanism 17 comprises a limit disc 171, a limit sleeve 172 and a limit rod 173, the limit disc 171 is fixedly connected to the right end of the connecting rod 7, the limit sleeve 172 is sleeved on the surface of the limit disc 171, the limit rod 173 is fixedly connected to the surface of the limit sleeve 172, one end of the limit rod 173, which is far away from the limit sleeve 172, is fixedly connected to the inside of the transmission sleeve 9, the shape of the surface of the transmission sleeve 9 and the shape of the surface of the sleeve 111 are both T-shaped, the sleeve 111 can be limited by setting the shape of the surface of the transmission sleeve 9 and the shape of the surface of the sleeve 111 to be T-shaped, the phenomenon that the sleeve 111 breaks away from the fixed sleeve 3 in use is avoided, the stability of the sleeve 111 in use is improved, and the input end of the fan 6 is communicated to the right side of the rotating sleeve 10 through a corrugated pipe.
When the extractor body 1 is in use, a user holds the rotating rod 16 and rotates the rotating rod 16 to drive the transmission sleeve 9 to rotate, the transmission sleeve 9 drives the connecting rod 7 to move leftwards through the limiting rod 173 and the limiting sleeve 172, then the fan 6 is started, the fan 6 pumps air in the extractor body 1 through the rotating sleeve 10 and the positioning tube 4, and the extractor body 1 is in a sealed state, so that the backing plate 112 can be pulled rightwards while the air is pumped, the backing plate 112 drives the sleeve 111 to stretch the tension spring 115, at the moment, the outside air enters the extractor body 1 through the air inlet pipe 2 and the air inlet groove 113, and the air in the extractor body 1 is purified through the air injection of the fan 6 into the air purification device 5, so that the effect of extracting and purifying volatilized air can be achieved.
In summary, this nucleic acid extractor, the fan 6 is through rotating cover 10 and positioning tube 4 to the inside air of extractor body 1 and is drawn, make outside air get into the inside of extractor body 1 through intake pipe 2 and air inlet 113, carry out purification treatment to the gas of taking out by air purification device 5 again, thereby possess the advantage that can take out purification to volatilized gas, the current nucleic acid extractor does not possess certain gas processing mechanism, when opening the nucleic acid extractor, the gaseous gas that the reagent volatilized just can release and cause air pollution, and leave the problem that partial gas in the nucleic acid extractor then easily causes the pollution to the reagent of next extraction nucleic acid, the practicality of nucleic acid extractor has been improved.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (2)
1. A nucleic acid extractor comprising an extractor body (1), characterized in that: the utility model discloses a device for extracting air from a rubber cushion, which comprises an air purification device, an air inlet pipe (2) is communicated with the left side of an extraction instrument body (1), a connecting rod (7) is transversely arranged in the air inlet pipe (2), a fixing sleeve (3) is fixedly connected with the inside of the air inlet pipe (2), a positioning pipe (4) is communicated with the right side of the extraction instrument body (1), an air purification device (5) is fixedly arranged at the top of the extraction instrument body (1), a fan (6) is fixedly connected with the right side of the extraction instrument body (1), the output end of the fan (6) is communicated with the left side of the bottom of the air purification device (5) through a connecting pipe, a connecting rod (7) is transversely arranged in the inside of the positioning pipe (4), a sealing sleeve (8) is fixedly connected with the left side of the surface of the connecting rod (7), the surface of the sealing sleeve (8) is in contact with the inner wall of the positioning pipe (4), a transmission sleeve (9) is in threaded connection with the right side of an inner cavity of the positioning pipe (4), a rotating sleeve (10) is arranged on the right side of the surface of the transmission sleeve (9), the input end of the fan (6) is communicated with a fan (6) at the top of the positioning pipe (4), the air inlet pipe (5), a sealing mechanism (112), a sealing mechanism (11) is arranged in the air inlet mechanism (112), a sealing mechanism (112), and a sealing mechanism (114), the sleeve (111) is slidably connected to the inside of the fixed sleeve (3), the base plate (112) is fixedly connected to the right side of the sleeve (111), the air inlet groove (113) is formed in the right side of the top and the bottom of the inner wall of the air inlet pipe (2), the tension spring (115) is fixedly connected to the right side of the inner wall of the sleeve (111), the left end of the tension spring (115) is fixedly connected to the left side of the inner wall of the fixed sleeve (3), the rubber sleeve I (114) is fixedly connected to the left side of the base plate (112), and the left side of the rubber sleeve I (114) is in contact with the inner wall of the air inlet pipe (2); the right end of the connecting rod (7) is provided with a connecting mechanism (17), the connecting mechanism (17) comprises a limiting disc (171), a limiting sleeve (172) and a limiting rod (173), the limiting disc (171) is fixedly connected to the right end of the connecting rod (7), the limiting sleeve (172) is sleeved on the surface of the limiting disc (171), the limiting rod (173) is fixedly connected to the surface of the limiting sleeve (172), one end, far away from the limiting sleeve (172), of the limiting rod (173) is fixedly connected to the inside of the transmission sleeve (9), and the shape of the surface of the transmission sleeve (9) and the shape of the surface of the sleeve (111) are T-shaped; the surface of the connecting rod (7) is fixedly connected with a sliding block (12), a sliding groove (13) is formed in the positioning tube (4), and the sliding block (12) is slidably connected in the sliding groove (13); the right side of rotating sleeve (10) inner wall fixedly connected with rubber cover two (15), the left side of rubber cover two (15) and the right side contact of driving sleeve (9), the top and the bottom of driving sleeve (9) all fixedly connected with be located bull stick (16) in the outside of registration arm (4).
2. The nucleic acid extractor of claim 1, wherein: the left end fixedly connected with baffle (14) of connecting rod (7), the right side fixed connection of baffle (14) is in the left side of seal cover (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910696199.9A CN110257372B (en) | 2019-07-30 | 2019-07-30 | Nucleic acid extraction instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910696199.9A CN110257372B (en) | 2019-07-30 | 2019-07-30 | Nucleic acid extraction instrument |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110257372A CN110257372A (en) | 2019-09-20 |
| CN110257372B true CN110257372B (en) | 2024-01-09 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910696199.9A Active CN110257372B (en) | 2019-07-30 | 2019-07-30 | Nucleic acid extraction instrument |
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| Country | Link |
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| CN (1) | CN110257372B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113416630B (en) * | 2021-06-21 | 2022-11-01 | 上海金鹏分析仪器有限公司 | Nucleic acid purification system with anti-pollution effect |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN206751853U (en) * | 2017-05-01 | 2017-12-15 | 任碧琼 | A kind of full-automatic nucleic acid extraction, amplification integrated apparatus |
| CN206902130U (en) * | 2017-07-03 | 2018-01-19 | 广州瑞能医学科技有限公司 | A kind of anti-pollution instrument for extracting nucleic acid |
| CN209046942U (en) * | 2018-08-29 | 2019-06-28 | 深圳市杰恩凯电子科技有限公司 | A kind of Gas controller with dust reduction capability |
-
2019
- 2019-07-30 CN CN201910696199.9A patent/CN110257372B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN206751853U (en) * | 2017-05-01 | 2017-12-15 | 任碧琼 | A kind of full-automatic nucleic acid extraction, amplification integrated apparatus |
| CN206902130U (en) * | 2017-07-03 | 2018-01-19 | 广州瑞能医学科技有限公司 | A kind of anti-pollution instrument for extracting nucleic acid |
| CN209046942U (en) * | 2018-08-29 | 2019-06-28 | 深圳市杰恩凯电子科技有限公司 | A kind of Gas controller with dust reduction capability |
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| CN110257372A (en) | 2019-09-20 |
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