CN217838928U

CN217838928U - Large-scale and quick extraction device for virus nucleic acid

Info

Publication number: CN217838928U
Application number: CN202121734308.0U
Authority: CN
Inventors: 杨柳; 崔玉凤; 徐鹏; 钟学良
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-07-28
Filing date: 2021-07-28
Publication date: 2022-11-18
Anticipated expiration: 2031-07-28

Abstract

The utility model relates to a large amount of, quick extraction element of virus nucleic acid, including syringe, filter head includes filter head main part and filter layer, filter head main part both ends opening and mid portion diameter are greater than the both ends diameter, the one end of filter head is pegged graft with the nipple of syringe, and it has an annular groove to open at filter head main part mid portion inner wall, in the recess was arranged in to the filter layer periphery, the filter layer comprises the silica membrane between the support filter element of both sides and two support filter elements. The sample extraction amount of the device can be increased to 3-5ml, which is equivalent to the total sample collection amount. The extraction process is simple, the complex operation of repeated centrifugal tube replacement is omitted, the time is saved, the RNA concentration is greatly improved, and therefore, the accurate result is more effectively and quickly provided.

Description

Large-scale and quick extraction device for virus nucleic acid

Technical Field

The utility model belongs to the technical field of medical product and specifically relates to a large amount of, quick extraction element of virus nucleic acid.

Background

Nucleic acid detection is a molecular biological test, typically nucleic acids used to detect pathogens, which can detect infection earlier, and can detect the presence of viruses in the blood prior to antibody production. The conventional extraction method of virus nucleic acid includes silica gel membrane adsorption column method and magnetic bead method. In both methods, the sample is placed in a sample preservation solution, and a part of the solution is extracted for detection. The extraction amount of the preservation solution of the two methods is generally not large, only about 200-300ul, and the nucleic acid extraction cannot be carried out on all collected samples, so that the concentration of the extracted nucleic acid is low. In actual work, nucleic acid cannot be extracted and detection cannot be effectively performed. It is desirable to improve the efficiency of nucleic acid extraction.

Disclosure of Invention

The utility model relates to a large amount of, quick extraction element of virus nucleic acid, the device can once only draw the nucleic acid in the sample in a large number.

The technical scheme of the utility model

The utility model provides a large amount of, quick extraction element of virus nucleic acid, includes the syringe, the filter head includes filter head main part and filter layer, filter head main part both ends opening and mid portion diameter are greater than both ends diameter, the one end of filter head is pegged graft with the nipple of syringe, and it has an annular groove to open at filter head main part mid portion inner wall, the recess is arranged in to the filter layer periphery, the filter layer comprises the silica membrane between the support filter disc of both sides and two support filter discs.

The utility model has the advantages that:

the extraction amount of the preservation solution of the device can be increased to 3-5ml, which is equivalent to the collection amount of all samples. The extraction process is simple, the complex operation of repeatedly centrifuging and changing tubes is omitted, the time is saved, the RNA concentration is greatly improved, and the accurate result is more effectively and quickly provided. Can be widely applied to clinical and scientific research units. And the utility model discloses need not special instrument and equipment, need not require the measurement personnel professional level, simple swift, save time also is applicable to the examination of a large amount of samples, and the result is accurate, and sensitivity is high, and the rate of accuracy is big.

Drawings

Fig. 1 is a schematic view of the overall structure of the present application.

Fig. 2 is a schematic view of a filter head structure according to the present application.

Fig. 3 is another schematic structural diagram of the filter head of the present application.

Detailed Description

The invention provides a device for quickly extracting a large amount of viral nucleic acid, which is used for quickly extracting the large amount of viral nucleic acid aiming at the problem of small sample extraction amount of the existing nucleic acid extraction device, so that all detection samples pass through a nucleic acid adsorption membrane (a silicon dioxide membrane) and are sucked by an injector, and the whole extraction process can be completed. And nucleic acid with higher concentration is finally extracted by pumping and cleaning, and the method is simple and convenient.

The principles and features of this invention are described below in conjunction with the following drawings, which are set forth by way of illustration only and are not intended to limit the scope of the invention.

As shown in FIG. 1, the device for large-scale and rapid extraction of viral nucleic acid comprises a syringe 1 and a filter head 2. The injector is used by the existing medical staff. The standard injector used in the experiment is 10mL, the total length of the syringe of the injector is 10.5cm, and the inner diameter of the barrel is 1.5cm.

The filter head 2 comprises a filter head main body 3 and a filter layer 4, the filter head main body 3 is of a hollow tubular structure, the optimal scheme selects a round tube structure, the diameter of the middle part 5 of the filter head main body 3 is larger than the diameters of the two ends, the inner wall of the middle part 5 of the filter head main body is provided with an annular groove 6, and the periphery of the filter layer 4 is arranged in the groove 6. One end of the filter head 2 is connected with the nipple 7 of the injector 1, and can be inserted or connected in other ways, and the filter head and the injector can be connected or separated at any time as long as the connection is realized.

The filter layer 4 is composed of support filters 8 on both sides and a silica membrane 9 between the two support filters 8. The silica membrane 9 is a filtration membrane for filtering and adsorbing nucleic acid, and the support filter sheets 8 on both sides are for protecting the silica membrane. The two side supporting filters 8 and the middle silicon dioxide film 9 can be fixed with each other on the periphery or clamped with each other and are arranged in the annular groove 6 in the filter head for filtering and adsorbing nucleic acid.

A second embodiment of the filter head body:

the filter head main part 3 comprises two parts, namely a first filter head main part 10 and a second filter head main part 11, the diameter of the large end of the diameter of the first filter head main part 10 is small, the small end of the diameter is connected with the nipple 7 of the injector, the inner wall of the large end of the diameter is provided with an annular groove 6, the diameter of the large end of the second filter head main part 11 is small, the large end of the diameter of the second filter head main part 11 is inserted into the large end of the first filter head main part 10, and the filter layer is clamped 4 in the groove of the first filter head main part. The first filter head body and the second filter head body are in close contact and can be connected in a plugging or sticking mode.

The application device using method (operating according to the following steps):

s1, further sucking nucleic acid extracting solution after sucking a sample by using an injector;

s2, mounting the filter head on the injector;

s3, pushing the syringe piston to discharge the mixture of the sample and the nucleic acid extracting solution from the filter head;

s4, pulling out the filter head;

s5, extracting the cleaning fluid by using an injector;

s6, mounting the filter head on the injector;

s7, discharging the cleaning liquid from the filter head;

s8, the pure nucleic acid is left in the filter head;

and S9, finally, pumping a small amount of eluent into the filter head by using a new injector to wash out the nucleic acid.

The utility model discloses can extract RNA in a large number fast. The utility model discloses use extensively, easily preparation and save the testing expense, simple to use detects fast, need not special instrument and equipment, does not require the measurement personnel to possess professional level, can simply effectively extract a large amount of RNA fast. The method is suitable for screening a large number of samples, simple, convenient and quick, and has high sensitivity and accurate and reliable result.

Claims

1. A device for large-scale and rapid extraction of viral nucleic acid, which is characterized in that: including the syringe, the filter head includes filter head main part and filter layer, the filter head main part is hollow tubular structure, and filter head main part middle part diameter is greater than both ends diameter, the one end of filter head is pegged graft with the nipple of syringe, and it has an annular groove to open at filter head main part middle part inner wall, in the recess was arranged in to the filter layer periphery, the filter layer comprises the silica membrane between the support filter element of both sides and two support filter elements.