CN211717930U - Sample purification and concentration device - Google Patents
Sample purification and concentration device Download PDFInfo
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- CN211717930U CN211717930U CN201921729453.2U CN201921729453U CN211717930U CN 211717930 U CN211717930 U CN 211717930U CN 201921729453 U CN201921729453 U CN 201921729453U CN 211717930 U CN211717930 U CN 211717930U
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- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The utility model discloses a sample purification enrichment facility, include: an end cover, a trough pipe, a filter membrane support, a filter membrane and a centrifuge pipe; the filter membrane support is characterized in that a feed liquid flow channel is arranged on the inner side of the filter membrane support, the outlet of the flow channel is directly communicated with the bottom, the feed trough pipe is provided with a sample containing cavity, a liquid outlet communicated with the sample containing cavity is formed in the outer side of the feed trough pipe, the filter membrane is fixed on the outer side of the feed trough pipe and covers the liquid outlet, the filter membrane support is tightly attached to the filter membrane and then vertically or obliquely fixed on the feed trough pipe, then the filter membrane support and the feed trough pipe are sleeved into a centrifugal tube together, the filter membrane support is tightly covered by an end cover, and the sample. The device carries out separation, cleaning, purification, filtration, desalination and concentration processes on a sample under the action of driving force or centrifugal force; in the operation process, other media are not needed, other pipetting devices are not needed in the operation process, no sample is lost, and extremely small amount of samples can be separated, purified, concentrated and the like.
Description
Technical Field
The utility model relates to a sample purification enrichment facility mainly used in biological product fields such as separation, purification, concentration, desalination.
Background
The centrifugal filter device is mainly used for separating, cleaning, concentrating, desalting and purifying biological product samples, such as urine, diagnostic reagents, monoclonal antibodies, blood plasma, spinal fluid, viruses and the like, has small treatment capacity and high concentration multiple, separates the samples, and has extremely important scientific research value on speed and recovery of residue samples in the process of filtering and purifying. However, the concentration purity of the currently marketed filtration centrifuge tube after purification and concentration is low, and the filtration centrifuge tube has large sample loss, is difficult to meet the high requirements of clinic and laboratories, and is often difficult to realize especially for sample treatment with small volume and multiple batches.
As sample volumes of proteins, plasmids, viruses, DNA, etc. become smaller, the potential for undesirable sample loss due to the retention volume within the device has become very important.
However, the conventional ultrafiltration centrifuge tube sold in the market at present has various defects, a certain inclination angle exists between the filter membrane and the feed liquid, the centrifuge belongs to dead-end filtration, concentration polarization is easily formed, intercepted substances are accumulated on the surface of the membrane, concentration is difficult to perform, meanwhile, the filtration time is long, the protein recovery rate is low, the product is easily damaged, meanwhile, the product sold in the market is manufactured by multiple processes, the filter membrane is fixed on the support plate and then connected with the sleeve, the membrane is easily damaged in the production process, the product yield is low, and the price is high; therefore, it is urgently needed to increase the filtration speed, simplify the production process flow and increase the product percent of pass so as to reduce the production cost.
Patent publication No. CN206924902U discloses a purification and concentration device, wherein filter membranes of the device are fixed at the inner side of a filter plate and then are vertically or obliquely fixed on the side wall of a filter membrane support or horizontally fixed at the bottom of the filter membrane support; the filter membrane is fixed on the filter membrane support and then welded on the material tank pipe, so that the membrane is easy to break and the yield is low; the feed liquid of the feed groove pipe penetrates through the membrane and then enters the feed groove of the supporting plate, the outlet is arranged on the back of the feed groove and is a pinhole, and the processing difficulty is high.
At present, in a purification and concentration device sold on the market, a certain inclination angle exists between a membrane and a feed liquid, molecules larger than the membrane pore diameter cannot penetrate through membrane pores under the action of an external driving force, and are easily enriched in the membrane pores to block the membrane pores, so that the concentration speed is low, and the separation and concentration failure is caused until the membrane pores are completely blocked.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a not enough to prior art, provide a sample purification enrichment facility, can carry out high power concentration to the sample stoste, and do not have the sample loss.
The purpose of the utility model is realized by the following technical scheme: a sample purification and concentration device comprising: the device comprises an end cover, a trough pipe, a filter membrane support, a filter membrane and a centrifuge tube, and is characterized in that a feed liquid flow channel is arranged on the inner side of the filter membrane support, an outlet of the flow channel is directly communicated with the bottom, the trough pipe is provided with a sample accommodating cavity, openings communicated with the sample accommodating cavity are formed in two sides of the trough pipe, the filter membrane is fixed on the outer opening of the trough pipe firstly, then the filter membrane support is tightly attached to the filter membrane and is vertically or obliquely fixed on the trough pipe, then the filter membrane support and the trough pipe are sleeved into the centrifuge tube together, the centrifuge tube is tightly covered by the end cover, the sample accommodating cavity, the filter membrane, the feed liquid flow channel and the centrifuge tube are sequentially communicated to form a purification concentration separation channel, a sample to be concentrated is added into the trough pipe, the feed liquid with the pore diameter smaller than that of the membrane penetrates through the membrane and.
Further, the end cover is a connected circular arc-shaped direct-pressing end cover or a threaded end cover.
Furthermore, the liquid drainage wire groove of the filter membrane support and the flow channel outlet communicated with the liquid drainage wire groove are in regular geometric shapes.
Further, the regular geometric shape is selected from the group consisting of a circle, a semicircle, an ellipse, an arc, a sector, and a line.
Furthermore, the liquid drainage wire groove of the filter membrane support and the flow passage outlet communicated with the liquid drainage wire groove are in irregular geometric shapes.
Furthermore, the filter membrane is selected from a flat nanofiltration membrane, an ultrafiltration membrane and a microfiltration membrane, the material of the filter membrane is selected from one or a mixture of two or more of polyethersulfone, polysulfone, polyvinylidene fluoride, cellulose diacetate, polypropylene, polyethylene, polytetrafluoroethylene, polyacrylonitrile, regenerated cellulose and polyamide, and the aperture of the filter membrane is selected from 1k, 3k, 5k, 10k, 30k, 50k, 100k, 300kd, 500kd, 0.1um, 0.22um, 0.45um, 0.65um, 1.2um, 3um, 5um, 10um and 20 um.
Further, the material groove pipe is in a ladder-shaped structure which is gradually reduced from the inlet along the radial direction.
Furthermore, the inclination angle of the plane where the liquid drainage line groove is located and the filter membrane is 0-12 degrees, the inclination angle is close to vertical, and the liquid drainage line groove is not easy to block during filtering.
Furthermore, the filter membrane support, the feed trough pipe and the centrifuge tube are subjected to super-hydrophobic and hydrophobic treatment before the filter membrane is welded.
Further, the super-hydrophobic and hydrophobic treated material is selected from hydrophobic and oleophobic agent and nano hydrophobic modified material.
The utility model has the advantages that:
the membrane is welded on a filter membrane support and then fixed on a material tank pipe, the method is easy to cause membrane rupture, the yield is low, the periphery of four sides is welded during welding, the dead area of a membrane is large, and a material liquid penetrating through a membrane hole enters a flow channel, flows out through a small hole on the back and then enters a centrifuge tube, so that the filter membrane and the filter membrane support have volume residues;
the utility model has the advantages that the membrane is not directly fixed and welded with the filter membrane support, the filter membrane is only physically and closely attached to the filter membrane to prevent the filter membrane from being broken under the action of driving force, the filter membrane is fixed on two sides of the trough pipe, the vertical design is adopted, the filter membrane is approximately parallel to the trough pipe, and the centrifugal filter has the biggest advantage that the flow direction of concentrated liquid during the concentration and separation of feed liquid is parallel to the filter membrane and is similar to cross-flow filtration, so that the blockage of membrane holes is not easily caused, and the centrifugal filter is essentially different from the dead-end filtration mode adopted by the similar products on the market at present; secondly, the filter membrane support adopts the runner export of linear type or irregular geometric shape, and directly communicates the bottom, all the former point or line flow through filtrating, is now that the feed liquid permeates the membrane after, the direct velocity of flow that filters is far faster than the velocity of flow device of the trompil in the back. So the design of the utility model adopts the present completely different design principle and changes the filtration mechanism of the concentration device. Meanwhile, the processing difficulty of the product is greatly reduced, the product percent of pass is improved, anti-adhesion treatment is performed on parts contacted with the sample, the sample is not polluted, the extremely high recovery rate of the sample is obtained, and the accuracy of a later analysis result is ensured.
Under the same appearance size, the membrane filtration area is increased, the device adopts a vertical membrane design under the action of centrifugal force, the probability of concentration polarization in the filtration process is greatly reduced, the filtration speed is increased, in the separation process, membrane holes are not easy to block, the purity and recovery efficiency of the separated product are high, meanwhile, the concentrate can be subjected to multistage separation, the use is convenient, and the sample hardly remains after the hydrophobic and oleophobic treatment, so that the secondary pollution is avoided;
according to different needs in actual use, the utility model has the key points that the filter membrane is directly fixed on the trough pipe, the filter membrane support is adhered to the membrane surface, the flow channel is designed on the filter membrane support, and the filtrate directly enters the centrifuge tube after passing through the filter membrane and the flow channel of the filter membrane support, thereby not only playing the role of supporting the membrane, but also having the function of the flow channel, and simultaneously not being directly fixed with the membrane, thereby greatly increasing the filtration area, greatly simplifying the technical difficulty of the product, and obtaining high recovery rate; the device is used for separating, cleaning, purifying, filtering, exchanging and concentrating, other media are not needed in the operation process, other liquid transfer devices are not needed in the operation process, no sample loss exists, and separation, purification, concentration and the like can be carried out on a very small amount of samples.
The utility model discloses can also be before the equipment, can be as required, earlier handle each subassembly with hydrophobic oleophobic agent, the processing mode can be handled 3-5min with commercially available hydrophobic oleophobic agent under temperature 150-.
The utility model discloses a product can carry out multistage separation with the concentrate under centrifuge's centrifugal force effect, convenient to use, and in the disengaging process, the difficult jam in membrane hole, and the product purity after the separation is high.
According to different needs during the in-service use, this device can also adopt other mounting structure forms commonly used, especially right the utility model discloses a key is that each subassembly designs alone, then assembles, has greatly simplified the product technology degree of difficulty, no matter be any rules such as circular, semicircle, pitch arc, fan-shaped pitch arc, line type and anomalous geometry, all belong to the utility model discloses a scope, in a word reach with basically the same means the utility model discloses a technical effect all should belong to the utility model discloses a protection scope.
Drawings
FIG. 1 is an exploded view of a purification and concentration apparatus with a threaded end cap;
FIG. 2 is a corresponding assembly view of the purification and concentration apparatus;
FIG. 3 is a view of the internal structure of the purification and concentration device at an angle corresponding to the threaded end cap;
FIG. 4 is a view of the internal structure of the purification and concentration device at another angle relative to the threaded end cap;
FIG. 5 is a structural arrangement diagram of feed liquid flow channels on the inner side surface of a filter membrane support used with a threaded end cover;
in the figure, an end cover 1, a trough pipe 2, a filter membrane support 3, a filter membrane 4, a centrifuge pipe 5, a feed liquid flow passage 6 and a flow passage outlet 7.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.
Example 1
As shown in fig. 1-5, the utility model provides a sample purification and concentration device, which comprises an end cover 1, a trough pipe 2, a filter membrane support 3, a filter membrane 4 and a centrifuge tube 5; the filter membrane support 3 inboard has feed liquid runner 6, and runner export 7 is straight-through bottom, the feed trough pipe 2 has sample holding chamber, and feed trough pipe 1 side is opened outward has the liquid outlet that is linked together with sample holding chamber, filter membrane 4 fixes in the outside of feed trough pipe 1 and covers the liquid outlet earlier, then pastes filter membrane 4 with filter membrane support 3 back vertical or slope fix on feed trough pipe 2, embolias the centrifuging tube 5 with feed trough pipe 2 afterwards in, and tightly lid through end cover 1 again, sample holding chamber, filter membrane 4, feed liquid runner and centrifuging tube 5 are linked together in proper order and constitute purification concentrated passageway.
In the embodiment of the application, the end cover 1 is a conjoined circular arc direct-pressing end cover or a threaded end cover. The material groove pipe is in a ladder-shaped structure which is gradually reduced from the inlet along the radial direction.
The angle of inclination that drainage wire casing 6 place plane and filter membrane 4 are 0 to 12 degrees, the filter membrane is little with the inclination of filtrate, be difficult to the polluted membrane, the production degree of difficulty has reduced, very big reduction the probability of concentration polarization in filtering process, increased filtration speed and separation process, the difficult jam in membrane hole, product purity and recovery efficiency after the separation are high, can carry out multistage separation with the concentrate simultaneously, high durability and convenient use, through hydrophobic oleophobic processing after-treatment device, the sample is almost not had and remains, do not cause secondary pollution.
The filter membrane is directly welded on the trough pipe, the operation is simple, and the filter membrane support only plays a supporting role. According to different requirements in actual use, the utility model is characterized in that the filter membrane is directly fixed on the trough pipe, the filter membrane support is tightly adhered to the membrane surface, and the flow channel is designed on the filter membrane support, thereby greatly increasing the filtration area, greatly simplifying the technical difficulty of the product, and obtaining high recovery rate; the device is used for separating, cleaning, purifying, filtering, exchanging and concentrating, other media are not needed in the operation process, other liquid transfer devices are not needed in the operation process, no sample loss exists, and separation, purification, concentration and the like can be carried out on a very small amount of samples. Therefore, the filter membrane is fixed on the inner side of the filter plate, and is vertically or obliquely fixed on the side wall of the filter membrane support or is horizontally fixed at the bottom of the filter membrane support, or the filter membrane 4 is fixed on the outer side of the feed trough pipe 1 and covers the liquid outlet, and the filter membrane support 3 is vertically or obliquely fixed on the feed trough pipe 2 after clinging to the filter membrane 4.
In the embodiment of the present application, the filter membrane support 3 has a liquid drainage groove 6 and a flow channel outlet 7 communicating with the liquid drainage groove 6, and the liquid drainage groove 6 can be, but not limited to, a circle, a semicircle, an ellipse, an arc, a sector, a line, and any other regular and irregular geometric shape. The flow channel outlet 7 is straight through the bottom and is linear.
In the embodiment of the application, the filter membrane 4 is selected from a flat nanofiltration membrane, an ultrafiltration membrane and a microfiltration membrane, the material of the filter membrane 4 is selected from one or a mixture of two or more of polyethersulfone, polysulfone, polyvinylidene fluoride, cellulose diacetate, polypropylene, polyethylene, polytetrafluoroethylene, polyacrylonitrile, regenerated cellulose and polyamide, and the pore diameter of the filter membrane 4 is selected from 1k, 3k, 5k, 10k, 30k, 50k, 100k, 300kd, 500kd, 0.1um, 0.22um, 0.45um, 0.65um, 1.2um, 3um, 5um, 10um and 20 um.
Except that the end cover 1 is made of a colored non-transparent material, other parts are made of transparent materials, and scales are marked on the trough pipe 2 and the centrifugal pipe 5; the feed trough pipe 2, the filter membrane support 3 and the centrifuge tube 5 can be firstly treated by super-hydrophobic and hydrophobic treatment, and the treatment materials can be but are not limited to hydrophobic and oleophobic agents and nano hydrophobic modified materials; the treatment mode can be that the commercial hydrophobic and oleophobic agent is used for treating for 3-5min at the temperature of 150-.
Adding a sample to be concentrated and separated by 15ml into a material groove pipe 2, sleeving the sample into a centrifugal tube 5, screwing an end cover 1, connecting the sample and the centrifugal tube into an integral filtering centrifugal tube, separating the feed liquid from a liquid outlet through a filter membrane 4 under the action of centrifugal force of a centrifugal machine, then flowing out through a liquid discharge groove 6 of a filter membrane support 3 on the back, entering the centrifugal tube 5 for collection, and retaining the concentrate in the material groove pipe 2,centrifugal speed of centrifugal machine 4000g, rotor swinging bucket type, 250And fixing the angle.
The device carries out separation, cleaning, purification, filtration, desalination and concentration processes on a sample under the action of driving force or centrifugal force; in the operation process, other media are not needed, other pipetting devices are not needed in the operation process, no sample is lost, and extremely small amount of samples can be separated, purified, concentrated and the like.
Table 1 shows the results of the test of the actual test of the finished product, which confirms that the device successfully filters the target sample and concentrates the sample to the dead volume at the bottom of the sample holder, the concentrated sample can be removed using a pipette or reverse, the filtrate that permeates through the membrane remains at the bottom of the centrifuge tube, different membrane pore sizes have different flow rates, e.g., 100kd has a faster flow rate than the 30kd device; the ability to concentrate the sample solution to a known concentration volume or dead volume conveys significant value to the user, using this device, the customer can concentrate their sample in a reliable manner that does not require them to continuously monitor the concentration process, essentially they can set the centrifuge speed and time, i.e., concentrate the separation as desired, when using a device that does not have this functionality, the user cannot control the concentration factor as desired, potentially allowing the sample to completely permeate the filter membrane resulting in test failure, inconvenient sample usefulness and subsequent analysis resulting in test failure
Table 1 shows the test data for the purification and concentration apparatus of the present invention, using ultrafiltration centrifuge tubes of 15ml gauge, using membranes of five typical molecular weights (3kd,10kd,30kd,50kd,100 kd); experimental data has confirmed the utility model discloses a device reaches and has surpassed the performance level of the like product sold in the market at present, and under most of the circumstances, the recovery of protein is greater than 95%, and these data show about using the experimental performance's of the sample performance test's of performance result, and it is experimental being concerned about the utility model discloses separation enrichment facility is optimized and is gone on the device after the hydrophobic processing at structural design, and is experimental to include: 1) testing the integrity; 2) dead volume; 3) total volume of recovery of sample.
TABLE 1
The integrity test of the device of the present invention tests the device for no air leakage at a pressure of 2bar, the limit being determined by randomly sampling the results of evaluating n-100 devices;
table 1 shows the results of the average dead volume measured when these devices were centrifuged for 15min, and the results show that five of the membrane-device configurations were able to achieve a dead volume within 15min, as expected, only one group did not reach the dead volume, and the remaining four groups all reached the concentration factor requirement during this centrifugation time, indicating that the smaller the pore size, the longer the time required to concentrate to the same factor, which is consistent with the expected experimental results, and the smaller pore size membranes required more time to filter the same dead volume.
TABLE 215 ml centrifugal Ultrafiltration apparatus for sodium chloride removal and recovery of protein solution comparative tests
Table 2 shows, the utility model discloses the whole data of device is superior to the like product of several foreign companies on the market comprehensively, also is superior to the product data of CN206924902U patent simultaneously, and this is mainly because the utility model discloses structural design optimizes, and the membrane direct fixation is in the feed trough pipe, and is vertical design, has reduced the emergence of the concentration polarization that the membrane produced when centrifugal separation, reduces the gathering of protein at its surface, simultaneously through hydrophobic treatment after, solution is difficult to adhere to at its surface, has greatly reduced the absorptive probability of protein to the rate of recovery of fabulous improvement protein also ensures high desalination.
Example 2
The same experimental procedure as in example 1 was used, with the difference that the initial volume was changed to 5ml using the smaller device of the present invention.
TABLE 35 ml centrifugal Ultrafiltration apparatus for sodium chloride removal and recovery of protein solution comparative tests
Table 3 shows, the comparison finds that the device of the present invention does not cause the change of protein recovery rate and salt rejection rate with the change of specification volume, and it also has the performance superior to the same kind of products on the market, and also superior to the performance of patent CN206924902U applied in the early period, and at the same time, the salt rejection rate does not depend on the concentration and volume of the sample, and the method of ultrafiltration is used to desalt without changing the composition of the buffer solution, and the dilution is repeated and then the filtration, and this process we call it as the equal volume ultrafiltration, and similar operations are repeated for many times, and the salt content of the solution can be obviously reduced to the minimum; if we want to dissolve the sample with different scoring buffers, we can use equal volume ultrafiltration for the purpose; after the sample is concentrated, the target buffer solution is added, and then dilution and concentration are repeatedly carried out.
Concentrating and separating spinal fluid, and especially separating and purifying specific protein. The device is particularly effective in the field of protein concentration, purification and separation, and can separate and purify protein from mixed products, such as cell or tissue sample fragments, serum protein, collagen and the like; the process may use smaller beads that bind specific proteins, extract and collect the extract from the beads for downstream analysis, assay studies, and the like.
The utility model discloses the device is under the effect of centrifugal force, and recovery efficiency is high, can carry out a lot of with the concentrate and separate, convenient to use, and in the disengaging process, the difficult jam in membrane hole, and the product purity after the separation is high. According to different needs when in-service use, the utility model discloses the device can also adopt other mounting structure forms commonly used, especially right the utility model discloses a key is that the runner export is changed into the line shape by the point, has greatly simplified the product technology degree of difficulty, no matter be any rules such as circular, semicircle, pitch arc, fan-shaped, line type and anomalous geometry, all belong to the utility model discloses a scope, in a word reach with basically the same means the utility model discloses a technical effect all should belong to the utility model discloses a protection scope.
Claims (10)
1. A sample purification and concentration device comprising: the device comprises an end cover, a trough pipe, a filter membrane support, a filter membrane and a centrifuge tube, and is characterized in that a feed liquid flow channel is arranged on the inner side of the filter membrane support, an outlet of the flow channel is directly communicated with the bottom, the trough pipe is provided with a sample accommodating cavity, openings communicated with the sample accommodating cavity are formed in two sides of the trough pipe, the filter membrane is fixed on the opening in the outer side of the trough pipe, the filter membrane support is tightly attached to the filter membrane and then vertically or obliquely fixed on the trough pipe, then the filter membrane support is sleeved into the centrifuge tube together with the trough pipe and tightly covered through the end cover, and the sample accommodating cavity, the filter membrane, the feed liquid flow.
2. The sample purification and concentration device according to claim 1, wherein the end cap is a one-piece circular arc-shaped direct-pressing end cap or a threaded end cap.
3. The apparatus for purifying and concentrating a sample according to claim 1, wherein the drainage groove of the filter membrane holder and the outlet of the flow channel communicating with the drainage groove have a regular geometric shape.
4. The apparatus for purifying and concentrating samples according to claim 3, wherein the regular geometric shape is selected from the group consisting of a circle, a semicircle, an ellipse, an arc, a sector, and a line.
5. The apparatus for purifying and concentrating a sample according to claim 1, wherein the drainage groove of the filter membrane holder and the outlet of the flow channel communicating with the drainage groove have irregular geometric shapes.
6. The apparatus for purifying and concentrating a sample according to claim 1, wherein the pore size of the filter membrane is selected from 1k, 3k, 5k, 10k, 30k, 50k, 100k, 300kd, 500kd, 0.1um, 0.22um, 0.45um, 0.65um, 1.2um, 3um, 5um, 10um, and 20 um.
7. The apparatus for purifying and concentrating a sample according to claim 1, wherein the trough tube has a trapezoidal shape gradually decreasing in a radial direction from the inlet.
8. The apparatus for purifying and concentrating samples as claimed in claim 1, wherein the inclination angle between the plane of the feed liquid flow channel and the filter membrane is 0 to 12 degrees.
9. The apparatus for purifying and concentrating samples as claimed in claim 1, wherein the filter membrane holder, the feed tank tube and the centrifuge tube are treated with super-hydrophobic and hydrophobic treatment before the filter membrane is welded.
10. The apparatus according to claim 1, wherein the material for super-hydrophobic and hydrophobic treatment is selected from hydrophobic and oleophobic agents and nano-hydrophobic modified materials.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921729453.2U CN211717930U (en) | 2019-10-15 | 2019-10-15 | Sample purification and concentration device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921729453.2U CN211717930U (en) | 2019-10-15 | 2019-10-15 | Sample purification and concentration device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211717930U true CN211717930U (en) | 2020-10-20 |
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| CN201921729453.2U Expired - Fee Related CN211717930U (en) | 2019-10-15 | 2019-10-15 | Sample purification and concentration device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110553894A (en) * | 2019-10-15 | 2019-12-10 | 杭州九龄科技有限公司 | sample purification enrichment facility |
| CN113670701A (en) * | 2021-08-20 | 2021-11-19 | 中国科学院西北生态环境资源研究院 | Gas purification device, and rare gas isotope measuring equipment and method |
-
2019
- 2019-10-15 CN CN201921729453.2U patent/CN211717930U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110553894A (en) * | 2019-10-15 | 2019-12-10 | 杭州九龄科技有限公司 | sample purification enrichment facility |
| CN113670701A (en) * | 2021-08-20 | 2021-11-19 | 中国科学院西北生态环境资源研究院 | Gas purification device, and rare gas isotope measuring equipment and method |
| CN113670701B (en) * | 2021-08-20 | 2024-01-23 | 中国科学院西北生态环境资源研究院 | Gas purification device, rare gas isotope measurement equipment and method thereof |
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