CN220099006U - Device for separating and removing floating oil components from adipose-derived mesenchymal stem cells - Google Patents
Device for separating and removing floating oil components from adipose-derived mesenchymal stem cells Download PDFInfo
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- CN220099006U CN220099006U CN202321671999.3U CN202321671999U CN220099006U CN 220099006 U CN220099006 U CN 220099006U CN 202321671999 U CN202321671999 U CN 202321671999U CN 220099006 U CN220099006 U CN 220099006U
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- mesenchymal stem
- adipose
- connecting block
- derived mesenchymal
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- 210000002901 mesenchymal stem cell Anatomy 0.000 title claims abstract description 24
- 238000007789 sealing Methods 0.000 claims abstract description 18
- 238000000926 separation method Methods 0.000 claims abstract description 17
- 229920003023 plastic Polymers 0.000 claims description 6
- 239000004033 plastic Substances 0.000 claims description 6
- 229920013716 polyethylene resin Polymers 0.000 claims description 3
- QQONPFPTGQHPMA-UHFFFAOYSA-N propylene Natural products CC=C QQONPFPTGQHPMA-UHFFFAOYSA-N 0.000 claims description 3
- 125000004805 propylene group Chemical group [H]C([H])([H])C([H])([*:1])C([H])([H])[*:2] 0.000 claims description 3
- 210000004027 cell Anatomy 0.000 abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 8
- 230000029087 digestion Effects 0.000 abstract description 5
- 238000001556 precipitation Methods 0.000 abstract description 2
- 230000001376 precipitating effect Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 3
- 210000000130 stem cell Anatomy 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 210000000577 adipose tissue Anatomy 0.000 description 2
- 102000029816 Collagenase Human genes 0.000 description 1
- 108060005980 Collagenase Proteins 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 229960002424 collagenase Drugs 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000002028 premature Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to the technical field of biology, in particular to a device for separating and removing floating oil from adipose-derived mesenchymal stem cells, which comprises a tube body, wherein a sealing cover is nested at the top of the tube body, and a graduated scale is arranged on the front surface of the tube body; the device is through setting up the body, puts fat mesenchymal stem cells in the inside of body, carries out centrifugal separation to fat mesenchymal stem cells, and the cell is after digestion separation, and water oil separates, and oil floats in the inside upper end of body, and water is in the below of oil, because the connecting block is the toper form, and the cell after the separation is precipitating the back, and the direct precipitation is at the lowest end at the connecting block for it is more convenient when water oil separates.
Description
Technical Field
The utility model relates to the technical field of biology, in particular to a device for separating and removing floating oil from adipose-derived mesenchymal stem cells.
Background
Adipose stem cells (ADSCs) are a type of stem cells isolated from adipose tissue that have multipotent differentiation potential. The method mainly recovers the repairing function of tissue cells, promotes the regeneration of the cells, fully improves the body functions while recovering the younger face, effectively improves diseases such as sub-health, premature senility and the like, truly and effectively resists aging from inside to outside, and needs to use a floating oil removing device when adipose-derived stem cells are separated;
when the adipose-derived mesenchymal stem cells are separated and obtained by using an adipose tissue collagenase digestion method, a large amount of floating oil exists in the tube after digestion and centrifugation, and the floating oil is directly poured or sucked out, so that wall-hanging residues are left, the floating oil is difficult to clean once, the centrifugation and washing are repeated, the working procedure is increased, the time is wasted, and certain damage is caused to the cells by repeated centrifugation;
therefore, we propose a new device for separating mesenchymal stem cells and removing floating oil, so as to solve the problems of difficult one-time cleaning, repeated centrifugal washing, increased working procedures and time waste in the prior art.
Disclosure of Invention
The utility model aims to provide a device for separating and removing floating oil from adipose-derived mesenchymal stem cells, which aims to solve the problems that the prior art is difficult to clean at one time, repeated centrifugal washing is performed, the working procedure is increased and the time is wasted.
In order to achieve the above purpose, the present utility model provides the following technical solutions: the device for separating and removing the floating oil content of the adipose-derived mesenchymal stem cells comprises a tube body, wherein a sealing cover is nested at the top of the tube body, and a graduated scale is arranged on the front surface of the tube body;
the inside embedding of body is provided with the pull rod, the bottom fixed mounting of pull rod has the stopper.
Preferably, the bottom of body fixed mounting has the connecting block, the inside of connecting block is hollow form, the bottom of connecting block is the toper form.
Preferably, the inner diameter of the tube body is 3cm, and the sum of the capacities of the tube body and the connecting block is 50ml.
Preferably, the diameter of the connecting block is consistent with the diameter of the pipe body, and the diameter of the bottommost end of the connecting block is smaller than 1.5cm.
Preferably, the diameter of the pull rod is 2mm, the length of the pull rod is 20cm, and the pull rod is made of hard plastic made of propylene.
Preferably, the limiting block is in an integrated cake shape, the diameter of the limiting block is 2.2cm, and the thickness of the limiting block is 0.5mm.
Preferably, the outer surface of the limiting block is provided with a sealing ring in a nested mode, the diameter of the sealing ring is 1mm, and the sealing ring is made of polyethylene resin soft plastic.
Compared with the prior art, the utility model has the beneficial effects that:
1. this kind of fat mesenchymal stem cell separation removes oil device that floats, through setting up the body, put fat mesenchymal stem cell in the inside of body, carry out centrifugal separation to fat mesenchymal stem cell, the cell is after digestion separation, and water oil separates, and oil floats in the inside upper end of body, and water is in the below of oil, because the connecting block is the toper form, the cell after the separation is at the extreme low at the connecting block after depositing, and direct precipitation for it is more convenient when water oil separates.
2. Secondly, this kind of fat mesenchymal stem cell separation removes oil removal device, through setting up the inside that the body was put into to the stopper to the pull rod, because the diameter of stopper is less, directly can put into the inside of connecting block, carry out spacingly through the cell of stopper to the connecting block bottom, the connecting block seals the cell of connecting block bottom through the sealing washer, because the diameter of stopper is greater than the diameter of connecting block bottom, when sealed, separate the space of bottom, prevent that the space of bottom is too little, cause the damage to the cell when sealed for the device is more convenient when wasing.
Drawings
FIG. 1 is a schematic view of a pipe body structure of the present utility model;
FIG. 2 is a schematic view of the overall structure of the present utility model;
FIG. 3 is a schematic diagram of the overall internal structure of the present utility model;
FIG. 4 is a schematic view of a stopper according to the present utility model;
in the figure: 1. a tube body; 101. a graduated scale; 102. a connecting block; 2. sealing cover; 3. a pull rod; 301. a limiting block; 302. and (3) sealing rings.
Detailed Description
Referring to fig. 1-2, in an embodiment of the present utility model;
example 1: the device for separating and removing the floating oil content of the adipose-derived mesenchymal stem cells comprises a tube body 1, wherein a sealing cover 2 is nested at the top of the tube body 1, and a graduated scale 101 is arranged on the front surface of the tube body 1;
a pull rod 3 is embedded in the pipe body 1, and a limiting block 301 is fixedly arranged at the bottom of the pull rod 3;
the bottom of the pipe body 1 is fixedly provided with a connecting block 102, the inside of the connecting block 102 is hollow, and the bottom of the connecting block 102 is conical;
the inner diameter of the pipe body 1 is 3cm, and the sum of the capacities of the pipe body 1 and the connecting block 102 is 50ml;
the diameter of the connecting block 102 is consistent with the diameter of the pipe body 1, and the diameter of the bottommost end of the connecting block 102 is smaller than 1.5cm;
when the device is used, the sealing cover is opened, the adipose-derived mesenchymal stem cells are placed in the tube body 1, centrifugal separation is carried out on the adipose-derived mesenchymal stem cells, after digestion and separation of the cells, water and oil are separated, oil floats at the upper end of the interior of the tube body 1, water is arranged below the oil, and as the connecting block 102 is conical, the separated cells are directly deposited at the lowest end of the connecting block 102 after being deposited, so that the device is more convenient and faster in water-oil separation.
Example 2: as can be seen by referring to fig. 2 to 4 of the specification, embodiment 2 differs from embodiment 1 in that:
the diameter of the pull rod 3 is 2mm, the length of the pull rod 3 is 20cm, and the pull rod 3 is made of hard plastic made of propylene;
the limiting block 301 is in an integrated cake shape, the diameter of the limiting block 301 is 2.2cm, and the thickness of the limiting block 301 is 0.5mm;
the outer surface of the limiting block 301 is provided with a sealing ring 302 in a nested mode, the diameter of the sealing ring 302 is 1mm, and the sealing ring 302 is made of polyethylene resin soft plastic;
after the cell deposits, the inside of body 1 is put into to stopper 302 through pull rod 3, because the diameter of stopper 302 is less, directly can put into the inside of connecting block 102, carry out the spacing through the cell of stopper 302 to connecting block 102 bottom, connecting block 102 seals the cell of connecting block 102 bottom through sealing washer 302, because the diameter of stopper 102 is greater than the diameter of connecting block 102 bottom, when sealed, separate the space of bottom, prevent that the space of bottom is too little, cause the damage to the cell when sealed for the device is more convenient when wasing.
Claims (7)
1. The device for separating and removing the floating oil content of the adipose-derived mesenchymal stem cells comprises a tube body (1), wherein a sealing cover (2) is nested at the top of the tube body (1), and a graduated scale (101) is arranged on the front surface of the tube body (1);
the pipe is characterized in that a pull rod (3) is embedded in the pipe body (1), and a limiting block (301) is fixedly arranged at the bottom of the pull rod (3).
2. The adipose-derived mesenchymal stem cell separation and oil removal device according to claim 1, wherein: the bottom of body (1) fixed mounting has connecting block (102), the inside of connecting block (102) is the cavity form, the bottom of connecting block (102) is the toper form.
3. The adipose-derived mesenchymal stem cell separation and oil removal device according to claim 1, wherein: the inner diameter of the pipe body (1) is 3cm, and the sum of the capacities of the pipe body (1) and the connecting block (102) is 50ml.
4. A adipose-derived mesenchymal stem cell separation and oil removal device according to claim 3, wherein: the diameter of the connecting block (102) is consistent with the diameter of the pipe body (1), and the diameter of the bottommost end of the connecting block (102) is smaller than 1.5cm.
5. The adipose-derived mesenchymal stem cell separation and oil removal device according to claim 1, wherein: the diameter of the pull rod (3) is 2mm, the length of the pull rod (3) is 20cm, and the pull rod (3) is made of hard plastic made of propylene.
6. The adipose-derived mesenchymal stem cell separation and oil removal device according to claim 1, wherein: the limiting block (301) is in an integral cake shape, the diameter of the limiting block (301) is 2.2cm, and the thickness of the limiting block (301) is 0.5mm.
7. The adipose-derived mesenchymal stem cell separation and oil removal device according to claim 1, wherein: the outer surface of the limiting block (301) is provided with a sealing ring (302) in a nested mode, the diameter of the sealing ring (302) is 1mm, and the sealing ring (302) is made of polyethylene resin soft plastic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321671999.3U CN220099006U (en) | 2023-06-28 | 2023-06-28 | Device for separating and removing floating oil components from adipose-derived mesenchymal stem cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321671999.3U CN220099006U (en) | 2023-06-28 | 2023-06-28 | Device for separating and removing floating oil components from adipose-derived mesenchymal stem cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220099006U true CN220099006U (en) | 2023-11-28 |
Family
ID=88870509
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321671999.3U Active CN220099006U (en) | 2023-06-28 | 2023-06-28 | Device for separating and removing floating oil components from adipose-derived mesenchymal stem cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220099006U (en) |
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2023
- 2023-06-28 CN CN202321671999.3U patent/CN220099006U/en active Active
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