CN108504554A - A kind of outer ovarian follicle collection of animal body - Google Patents
A kind of outer ovarian follicle collection of animal body Download PDFInfo
- Publication number
- CN108504554A CN108504554A CN201810507644.8A CN201810507644A CN108504554A CN 108504554 A CN108504554 A CN 108504554A CN 201810507644 A CN201810507644 A CN 201810507644A CN 108504554 A CN108504554 A CN 108504554A
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- Prior art keywords
- receiving flask
- liquid pipe
- air intake
- intake duct
- hose
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- Life Sciences & Earth Sciences (AREA)
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- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
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- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
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- Biomedical Technology (AREA)
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- General Engineering & Computer Science (AREA)
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Abstract
The invention discloses ovarian follicle collections outside a kind of animal body, including collector, hose, metal tube, buffering liquid pipe, thin plug, air intake duct, rubber stopper and receiving flask, the collector is made of handle, concave shaped cavity, rubber ring, upper cover and several prickers, upper cover is connect with hose, and hose is connect with metal tube；Buffering liquid pipe is made of the syringe adapter of upper end, intermediate tube body and the bulb syringe of lower end, and bulb syringe is equipped with several apertures；Rubber stopper fills in the bottleneck of receiving flask, and rubber stopper is inserted into one end of metal tube, buffering liquid pipe and air intake duct, and exposes in the top of receiving flask；The other end of air intake duct can be connect with vacuum pump, and the lower end of receiving flask carries glass valves, has fluid hole on glass valves.The outer ovarian follicle collection of animal body of the present invention, is easily and efficiently in vitro acquired the liquor folliculi of animal, does not easily cause the pollution of sample in operating process, very convenient to the cleaning of pipeline.
Description
Technical field
The invention belongs to test equipment technology field, it is related to a kind of outer ovarian follicle collection of animal body.
Background technology
Animal embryo technology is quickly grown, and the demand to a large amount of animal oocytes increasingly increases.Animal oocyte
Acquisition, mostly by collecting liquor folliculi on ovary after butchering, then picking egg mother cell is realized from liquor folliculi.It collects at present
The method of liquor folliculi mainly has stripping method, punctures method, blade patterning method, syringe suction method and vacuum pump acquisition method.
Stripping method punctures that method, these three methods of blade patterning method are cumbersome, and time-consuming, and the egg mother cell of acquisition is few.
Picker commonly uses a holding ovary, another hand takes syringe, using injection when using syringe suction method
The liquor folliculi for the negative pressure acquisition Ovarian surface that device generates, makes egg mother cell and liquor folliculi collect in syringe together, then adopting
The ovarian follicle liquid mixture collected is picked up in ovum ware from being pushed in syringe, and job step is cumbersome, is taken more.
Although operation of the vacuum pump acquisition method than syringe suction method slightly improves, and reduces the fussy degree of experiment,
But it does not have relevant cleaning system, this is not easy to carry out separation acquisition to multiple samples simultaneously.
Existing technology (CN201520570299), to scratch ovarian follicle, then liquor folliculi is carried out using multiple parallel scrapers
It collects.Although the technology can destroy ovarian follicle wall within the regular hour, liquor folliculi is made quickly to flow out, improved work efficiency,
But needed with other container collections after liquor folliculi outflow, this process be easy to cause the loss and pollution of liquor folliculi, in addition, scraping
Knife can equally scratch corpus luteum, its content is made to be mixed with liquor folliculi, to subsequently picking up chalaza carrys out very big difficulty.
The method pair that the prior art (CN201120314165 and CN201420190184) is combined using syringe needle and negative pressure
Liquor folliculi is collected, and liquor folliculi loss is few in operating process, is not easy to pollute.But these technologies only have single syringe needle,
Collect the less efficient of liquor folliculi；The length of syringe needle is not fixed, and easy inserting needle is too deep, is not easy to draw liquor folliculi；It is long
Needle be easy operator is damaged；It is not provided with the function of being cleaned to entire collector, is not easy to multiple samples
Separation acquisition.
This patent provides a kind of quickly extraction egg mother cell device, and not only job step is few for the device, but also easy to operate,
It takes short.
Invention content
Problem to be solved by this invention be to answer market urgently, overcome the deficiencies of the prior art and provide a kind of operation letter
Just, the device of antipollution quick extraction liquor folliculi.
The present invention adopts the following technical solutions realizes, a kind of animal body outer ovarian follicle collection, including collector, soft
Pipe, metal tube, buffering liquid pipe, thin plug, air intake duct, rubber stopper and receiving flask, the collector by handle, concave shaped cavity, rubber ring,
Upper cover and several prickers form, and pricker is fixed on concave shaped cavity lower part, and concave shaped cavity is fixed by the upper cover of spiral shell button and the upper end；On
Lid is connect with hose, and hose is connect with metal tube；Buffering liquid pipe is rushed by the syringe adapter of upper end, intermediate tube body and lower end
Ball composition is washed, bulb syringe is equipped with several apertures；Rubber stopper fills in the bottleneck of receiving flask, metal tube, buffering liquid pipe and air-breathing
Rubber stopper is inserted into one end of pipe, and exposes in the top of receiving flask；The upper end of liquid pipe is buffered at work by carefully filling in closing；It inhales
The other end of tracheae can be connect with vacuum pump, and the lower end of receiving flask carries glass valves, has fluid hole on glass valves.
Preferably, the end of air intake duct, buffering liquid pipe and metal tube is arranged from high to low in receiving flask.
The outer ovarian follicle collection of animal body of the present invention, is easily and efficiently in vitro acquired the liquor folliculi of animal, grasps
The pollution of sample is not easily caused during making, it is very convenient to the cleaning of pipeline.
Description of the drawings
It further illustrates with reference to the accompanying drawings and detailed description：
Fig. 1 is the structural schematic diagram of the outer ovarian follicle collection of animal body of the embodiment of the present invention.
Fig. 2 is the structural schematic diagram for buffering liquid pipe.
Fig. 3 is the structural schematic diagram that collector removes handle.
Fig. 4 is the assembling schematic diagram that collector removes handle
Fig. 5 is the ground plan that collector removes handle.
Fig. 6 is the overall structure figure of the outer ovarian follicle collection of animal body of inventive embodiments.
Specific implementation mode
As shown in figures 1 to 6, the present invention provides a kind of outer ovarian follicle collection of animal body, including collector, hose 9, metal
Pipe 10, buffering liquid pipe 6, carefully plug 13, air intake duct 12, rubber stopper 11 and receiving flask 7；
Collector is made of handle 4, concave shaped cavity 5, rubber ring 2, upper cover 3 and several prickers 1；Liquid pipe 6 is buffered by upper end
Syringe adapter 61, intermediate tube body 62 and the bulb syringe 63 of lower end form, bulb syringe 63 is equipped with several apertures 64；It receives
The lower end for collecting bottle 7 carries glass valves 8, has fluid hole on glass valves 8.
Pricker 1 is fixed on 5 lower part of concave shaped cavity；Concave shaped cavity 5 is fixed by the upper cover 3 of spiral shell button and the upper end；Upper cover 3 and hose
9 connections；Hose 9 is connect with metal tube 10；Rubber stopper 11 fills in the bottleneck of receiving flask 7；Metal tube 10, buffering liquid pipe 6 and air-breathing
Rubber stopper 11 is inserted into one end of pipe 12, and exposes in the top of receiving flask, the end of three in receiving flask 7 from high to low
Sequence is air intake duct 12, buffering liquid pipe 6 and metal tube 10；The upper end of liquid pipe 6 is buffered at work by carefully filling in 13 closings；Air intake duct
12 other end can be connect with vacuum pump.
The negative pressure that vacuum pump generates is transmitted to the pricker 1 on collector through receiving flask 6, metal tube 10, hose 9, and pricker 1 is logical
It crosses mechanical force and punctures ovarian follicle, while liquor folliculi is drawn under the action of negative pressure.The present invention has liberated the hand of operating personnel；Make ovarian follicle
Liquid with contacting external air, does not reduce its contaminated possibility；Multiple prickers work together the speed for improving acquisition, make
Egg mother cell be in the time in external environment be reduced to it is minimum.
The application method of the outer ovarian follicle collection of animal body of the present invention：
The all parts of ovarian follicle collection are connected, glass valves is closed, with thin plug Block buffer pipe；
Vacuum pump is connected in the outer end of air intake duct, vacuum pump is opened, air-breathing is made to reach stable state；
One holds collected animal ovary, and pricker is directed at ovarian follicle, to wherein by the handle of another portable collector
Liquor folliculi be acquired, until all ovarian follicles in liquor folliculi sucked；
Vacuum pump is closed, the vacuum pump line being connect with air intake duct is pulled up；
Glass valves is opened, with collected liquor folliculi in suitable container collection receiving flask；
Vacuum pump and each component are reconnected, buffer solution is drawn with collector, pricker and pipeline inner wall is cleaned, together
The face that Shi Qingxi lug cavities are contacted with ovarian follicle；
Vacuum pump is closed, the vacuum pump line being connect with air intake duct is pulled up；
Glass valves is opened, the buffer solution of flushing is discharged；
Suitable buffer solution is drawn with a syringe, buffer solution is connected to the syringe adapter of buffering liquid pipe upper end, it is right
Receiving flask is cleaned.
Finally it should be noted that：The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention,
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art, still may be used
With technical scheme described in the above embodiments is modified or equivalent replacement of some of the technical features.
All within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in the present invention's
Within protection domain.
Claims (2)
1. a kind of outer ovarian follicle collection of animal body, which is characterized in that including collector, hose, metal tube, buffer liquid pipe, thin
Plug, air intake duct, rubber stopper and receiving flask, the collector are made of handle, concave shaped cavity, rubber ring, upper cover and several prickers,
Pricker is fixed on concave shaped cavity lower part, and concave shaped cavity is fixed by the upper cover of spiral shell button and the upper end, and upper cover is connect with hose, hose and gold
Belong to pipe connection；Buffering liquid pipe is made of the syringe adapter of upper end, intermediate tube body and the bulb syringe of lower end, and bulb syringe is equipped with
Several apertures；Rubber stopper fills in the bottleneck of receiving flask, and rubber stopper is inserted into one end of metal tube, buffering liquid pipe and air intake duct,
And expose in the top of receiving flask；The upper end of liquid pipe is buffered at work by carefully filling in closing；The other end of air intake duct can with it is true
The lower end of sky pump connection, receiving flask carries glass valves, has fluid hole on glass valves.
2. the outer ovarian follicle collection of animal body according to claim 1, which is characterized in that air intake duct, buffering liquid pipe and gold
The end for belonging to pipe is arranged from high to low in receiving flask.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810507644.8A CN108504554A (en) | 2018-05-24 | 2018-05-24 | A kind of outer ovarian follicle collection of animal body |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810507644.8A CN108504554A (en) | 2018-05-24 | 2018-05-24 | A kind of outer ovarian follicle collection of animal body |
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| Publication Number | Publication Date |
|---|---|
| CN108504554A true CN108504554A (en) | 2018-09-07 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810507644.8A Pending CN108504554A (en) | 2018-05-24 | 2018-05-24 | A kind of outer ovarian follicle collection of animal body |
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| CN (1) | CN108504554A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109731153A (en) * | 2019-01-24 | 2019-05-10 | 山东农业大学 | One kind can quickly extract egg mother cell and save transport device |
| WO2019186547A1 (en) * | 2018-03-26 | 2019-10-03 | Carmel Diagnostics Ltd. | Follicular content storage |
| RU2811295C1 (en) * | 2023-06-16 | 2024-01-11 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Санкт-Петербургский государственный аграрный университет" (ФГБОУ ВО СПбГАУ) | Tool for isolating oocyte-cumulus complexes from mammalian ovaries |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008099841A (en) * | 2006-10-18 | 2008-05-01 | Shizuoka Prefecture | Vibration type ovum sampler and ovum wash recovery device |
| CN202313662U (en) * | 2011-08-26 | 2012-07-11 | 湖北省农业科学院畜牧兽医研究所 | Device for extracting oocytes quickly |
| CN203468709U (en) * | 2013-10-12 | 2014-03-12 | 赵鹏 | Membrane-rupturing and liquid-absorbing device for gynaecology and obstetrics |
| CN204766038U (en) * | 2015-07-31 | 2015-11-18 | 中国农业科学院特产研究所 | Ovum bubble scraper |
| CN105233353A (en) * | 2015-11-18 | 2016-01-13 | 王声 | Negative pressure drainage device for breast surgery |
| CN107438394A (en) * | 2015-03-02 | 2017-12-05 | 威里利生命科学有限责任公司 | Automatic blood sampling apparatus |
-
2018
- 2018-05-24 CN CN201810507644.8A patent/CN108504554A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008099841A (en) * | 2006-10-18 | 2008-05-01 | Shizuoka Prefecture | Vibration type ovum sampler and ovum wash recovery device |
| CN202313662U (en) * | 2011-08-26 | 2012-07-11 | 湖北省农业科学院畜牧兽医研究所 | Device for extracting oocytes quickly |
| CN203468709U (en) * | 2013-10-12 | 2014-03-12 | 赵鹏 | Membrane-rupturing and liquid-absorbing device for gynaecology and obstetrics |
| CN107438394A (en) * | 2015-03-02 | 2017-12-05 | 威里利生命科学有限责任公司 | Automatic blood sampling apparatus |
| CN204766038U (en) * | 2015-07-31 | 2015-11-18 | 中国农业科学院特产研究所 | Ovum bubble scraper |
| CN105233353A (en) * | 2015-11-18 | 2016-01-13 | 王声 | Negative pressure drainage device for breast surgery |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019186547A1 (en) * | 2018-03-26 | 2019-10-03 | Carmel Diagnostics Ltd. | Follicular content storage |
| CN109731153A (en) * | 2019-01-24 | 2019-05-10 | 山东农业大学 | One kind can quickly extract egg mother cell and save transport device |
| CN109731153B (en) * | 2019-01-24 | 2021-06-08 | 山东农业大学 | Can draw oocyte fast and preserve conveyer |
| RU2811295C1 (en) * | 2023-06-16 | 2024-01-11 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Санкт-Петербургский государственный аграрный университет" (ФГБОУ ВО СПбГАУ) | Tool for isolating oocyte-cumulus complexes from mammalian ovaries |
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