CN208532790U - A kind of outer ovarian follicle collection of animal body - Google Patents
A kind of outer ovarian follicle collection of animal body Download PDFInfo
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- CN208532790U CN208532790U CN201820783377.2U CN201820783377U CN208532790U CN 208532790 U CN208532790 U CN 208532790U CN 201820783377 U CN201820783377 U CN 201820783377U CN 208532790 U CN208532790 U CN 208532790U
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- receiving flask
- liquid pipe
- air intake
- intake duct
- hose
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Abstract
The utility model discloses ovarian follicle collections outside a kind of animal body, including collector, hose, metal tube, buffering liquid pipe, thin plug, air intake duct, rubber stopper and receiving flask, the collector is made of handle, concave shaped cavity, rubber ring, upper cover and several prickers, upper cover is connect with hose, and hose is connect with metal tube；Buffering liquid pipe is made of the syringe adapter of upper end, intermediate tube body and the bulb syringe of lower end, and bulb syringe is equipped with several apertures；Rubber stopper fills in the bottleneck of receiving flask, and rubber stopper is inserted into one end of metal tube, buffering liquid pipe and air intake duct, and exposes in the top of receiving flask；The other end of air intake duct can be controlled to a vacuum pump, and the lower end of receiving flask has glass valves, have fluid hole on glass valves.The outer ovarian follicle collection of the utility model animal body, is easily and efficiently in vitro acquired the liquor folliculi of animal, does not easily cause the pollution of sample in operating process, very convenient to the cleaning of pipeline.
Description
Technical field
The utility model belongs to experiment equipment technology field, is related to a kind of outer ovarian follicle collection of animal body.
Background technique
Animal embryo technology is quickly grown, and the demand to a large amount of animal oocytes increasingly increases.Animal oocyte
Acquisition, multi-pass is crossed butcher after collect liquor folliculi on ovary, then picking egg mother cell is realized from liquor folliculi.It collects at present
The method of liquor folliculi mainly has stripping method, punctures method, blade patterning method, syringe suction method and vacuum pump acquisition method.
Stripping method punctures that method, these three methods of blade patterning method are cumbersome, and time-consuming, and the egg mother cell of acquisition is few.
Picker commonly uses a holding ovary, another hand takes syringe, using injection when using syringe suction method
The liquor folliculi for the negative pressure acquisition Ovarian surface that device generates, collects egg mother cell and liquor folliculi in syringe together, then adopting
The ovarian follicle liquid mixture collected is picked up in ovum ware from being pushed in syringe, and job step is cumbersome, time-consuming more.
Although operation of the vacuum pump acquisition method than syringe suction method slightly improves, and reduces the fussy degree of experiment,
But it does not have relevant cleaning system, this is not easy to carry out separation acquisition to multiple samples simultaneously.
Existing technology (CN201520570299) scratches ovarian follicle using multiple parallel scrapers, then liquor folliculi is carried out
It collects.Although the technology can destroy ovarian follicle wall within the regular hour, flow out liquor folliculi quickly, improve work efficiency,
But need after liquor folliculi outflow with other container collections, this process be easy to cause the loss and pollution of liquor folliculi, in addition, scraping
Knife can equally scratch corpus luteum, mix its content with liquor folliculi, carry out very big difficulty to subsequent chalaza of picking up.
The method pair that the prior art (CN201120314165 and CN201420190184) is combined using syringe needle and negative pressure
Liquor folliculi is collected, and liquor folliculi loss is few in operating process, is not easy to pollute.But these technologies only have single syringe needle,
The efficiency for collecting liquor folliculi is lower；The length of syringe needle is not fixed, and easy inserting needle is too deep, is not easy to draw liquor folliculi；It is too long
Needle be easy operator is damaged；It is not provided with the function of being cleaned to entire collector, is not easy to multiple samples
Separation acquisition.
This patent provides a kind of quickly extraction egg mother cell device, and not only job step is few for the device, but also easy to operate,
It is time-consuming short.
Utility model content
Problem to be solved in the utility model be to answer market urgently, overcome the deficiencies of the prior art and provide a kind of behaviour
Make the device of easy, antipollution quick extraction liquor folliculi.
The utility model adopts the following technical solutions realization, a kind of outer ovarian follicle collection of animal body, including acquisition
Device, hose, metal tube, buffering liquid pipe, thin plug, air intake duct, rubber stopper and receiving flask, the collector is by handle, concave shaped cavity, rubber
Cushion rubber, upper cover and several prickers composition, pricker are fixed on concave shaped cavity lower part, and concave shaped cavity is solid by the upper cover of spiral shell button and the upper end
It is fixed；Upper cover is connect with hose, and hose is connect with metal tube；Buffer liquid pipe by the syringe adapter of upper end, intermediate tube body and under
The bulb syringe at end forms, and bulb syringe is equipped with several apertures；Rubber stopper fills in the bottleneck of receiving flask, metal tube, buffering liquid pipe
It is inserted into rubber stopper with one end of air intake duct, and is exposed in the top of receiving flask；The upper end of liquid pipe is buffered at work by carefully filling in
Closing；The other end of air intake duct can be controlled to a vacuum pump, and the lower end of receiving flask has glass valves, have fluid hole on glass valves.
Preferably, the end of air intake duct, buffering liquid pipe and metal tube is arranged from high to low in receiving flask.
The outer ovarian follicle collection of the utility model animal body, easily and efficiently in vitro adopts the liquor folliculi of animal
Collect, does not easily cause the pollution of sample in operating process, it is very convenient to the cleaning of pipeline.
Detailed description of the invention
It further illustrates with reference to the accompanying drawings and detailed description:
Fig. 1 is the structural schematic diagram of the outer ovarian follicle collection of animal body of the utility model embodiment.
Fig. 2 is the structural schematic diagram for buffering liquid pipe.
Fig. 3 is the structural schematic diagram that collector removes handle.
Fig. 4 is the assembling schematic diagram that collector removes handle
Fig. 5 is the ground plan that collector removes handle.
Fig. 6 is the overall structure figure of the outer ovarian follicle collection of animal body of utility model embodiment.
Specific embodiment
As shown in figures 1 to 6, the utility model provides a kind of animal body outer ovarian follicle collection, including collector, hose 9,
Metal tube 10, buffering liquid pipe 6, carefully plug 13, air intake duct 12, rubber stopper 11 and receiving flask 7；
Collector is made of handle 4, concave shaped cavity 5, rubber ring 2, upper cover 3 and several prickers 1；Liquid pipe 6 is buffered by upper end
Syringe adapter 61, intermediate tube body 62 and the bulb syringe 63 of lower end form, bulb syringe 63 is equipped with several apertures 64；It receives
The lower end for collecting bottle 7 has glass valves 8, has fluid hole on glass valves 8.
Pricker 1 is fixed on 5 lower part of concave shaped cavity；Concave shaped cavity 5 is fixed by the upper cover 3 of spiral shell button and the upper end；Upper cover 3 and hose
9 connections；Hose 9 is connect with metal tube 10；Rubber stopper 11 fills in the bottleneck of receiving flask 7；Metal tube 10, buffering liquid pipe 6 and air-breathing
Rubber stopper 11 is inserted into one end of pipe 12, and expose in the top of receiving flask, the end of three in receiving flask 7 from high to low
Sequence is air intake duct 12, buffering liquid pipe 6 and metal tube 10；The upper end of liquid pipe 6 is buffered at work by carefully filling in 13 closings；Air intake duct
12 other end can be controlled to a vacuum pump.
The negative pressure that vacuum pump generates is transmitted to the pricker 1 on collector through receiving flask 6, metal tube 10, hose 9, and pricker 1 is logical
It crosses mechanical force and punctures ovarian follicle, while drawing liquor folliculi under the action of negative pressure.The utility model has liberated the hand of operator；Make
Liquor folliculi with contacting external air, does not reduce its contaminated possibility；Multiple prickers work together the speed for improving acquisition
Degree, make egg mother cell be in the time in external environment be reduced to it is minimum.
The application method of the outer ovarian follicle collection of the utility model animal body:
The all parts of ovarian follicle collection are connected, glass valves is closed, with thin plug Block buffer pipe；
Vacuum pump is connected in the outer end of air intake duct, vacuum pump is opened, air-breathing is made to reach stable state；
One holds collected animal ovary, and pricker is directed at ovarian follicle, to wherein by the handle of another portable collector
Liquor folliculi be acquired, until all ovarian follicles in liquor folliculi sucked；
Vacuum pump is closed, the vacuum pump line connecting with air intake duct is pulled up；
Glass valves is opened, with collected liquor folliculi in suitable container collection receiving flask；
Vacuum pump and each component are reconnected, buffer is drawn with collector, pricker and pipeline inner wall is cleaned, together
The face that Shi Qingxi lug cavity is contacted with ovarian follicle；
Vacuum pump is closed, the vacuum pump line connecting with air intake duct is pulled up；
Glass valves is opened, the buffer of flushing is discharged；
Suitable buffer is drawn with a syringe, buffer is connected to the syringe adapter of buffering liquid pipe upper end, it is right
Receiving flask is cleaned.
Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention, it is not limited to this
Utility model, although the utility model is described in detail with reference to the foregoing embodiments, for those skilled in the art
For, it is still possible to modify the technical solutions described in the foregoing embodiments, or to part of technical characteristic
It is equivalently replaced.Within the spirit and principle of the utility model, any modification, equivalent replacement, improvement and so on,
It should be included within the scope of protection of this utility model.
Claims (2)
1. a kind of outer ovarian follicle collection of animal body, which is characterized in that including collector, hose, metal tube, buffer liquid pipe, thin
Plug, air intake duct, rubber stopper and receiving flask, the collector are made of handle, concave shaped cavity, rubber ring, upper cover and several prickers,
Pricker is fixed on concave shaped cavity lower part, and concave shaped cavity is fixed by the upper cover of spiral shell button and the upper end, and upper cover is connect with hose, hose and gold
Belong to pipe connection；Buffering liquid pipe is made of the syringe adapter of upper end, intermediate tube body and the bulb syringe of lower end, and bulb syringe is equipped with
Several apertures；Rubber stopper fills in the bottleneck of receiving flask, and rubber stopper is inserted into one end of metal tube, buffering liquid pipe and air intake duct,
And expose in the top of receiving flask；The upper end of liquid pipe is buffered at work by carefully filling in closing；The other end of air intake duct can with it is true
Sky pump connection, the lower end of receiving flask have glass valves, have fluid hole on glass valves.
2. the outer ovarian follicle collection of animal body according to claim 1, which is characterized in that air intake duct, buffering liquid pipe and gold
The end for belonging to pipe is arranged from high to low in receiving flask.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820783377.2U CN208532790U (en) | 2018-05-24 | 2018-05-24 | A kind of outer ovarian follicle collection of animal body |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820783377.2U CN208532790U (en) | 2018-05-24 | 2018-05-24 | A kind of outer ovarian follicle collection of animal body |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208532790U true CN208532790U (en) | 2019-02-22 |
Family
ID=65394043
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820783377.2U Expired - Fee Related CN208532790U (en) | 2018-05-24 | 2018-05-24 | A kind of outer ovarian follicle collection of animal body |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208532790U (en) |
-
2018
- 2018-05-24 CN CN201820783377.2U patent/CN208532790U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190222 Termination date: 20190524 |
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| CF01 | Termination of patent right due to non-payment of annual fee |