CN202313662U - Device for extracting oocytes quickly - Google Patents

Device for extracting oocytes quickly Download PDF

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Publication number
CN202313662U
CN202313662U CN2011203141658U CN201120314165U CN202313662U CN 202313662 U CN202313662 U CN 202313662U CN 2011203141658 U CN2011203141658 U CN 2011203141658U CN 201120314165 U CN201120314165 U CN 201120314165U CN 202313662 U CN202313662 U CN 202313662U
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CN
China
Prior art keywords
tube
puncture needle
oocytes
oocyte
collection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN2011203141658U
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Chinese (zh)
Inventor
肖红卫
乔宪凤
李莉
华文君
张立苹
毕延震
华再东
吴艳
魏金涛
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institute of Animal Science and Veterinary of Hubei Academy of Agricultural Sciences
Original Assignee
Institute of Animal Science and Veterinary of Hubei Academy of Agricultural Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institute of Animal Science and Veterinary of Hubei Academy of Agricultural Sciences filed Critical Institute of Animal Science and Veterinary of Hubei Academy of Agricultural Sciences
Priority to CN2011203141658U priority Critical patent/CN202313662U/en
Application granted granted Critical
Publication of CN202313662U publication Critical patent/CN202313662U/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Abstract

A device for extracting oocytes quickly comprises a collection tube, a tube plug, a puncture needle and an air duct, wherein an infusion tube between the collection tube and the puncture needle penetrates through the tube plug. The device is characterized in that the infusion tube and the puncture needle are integrated into a whole, the length of the infusion tube ranges from 3cm to 5cm, and the infusion tube and the puncture needle are arranged in a line, or an angle is formed between the infusion tube and the puncture needle. The oocytes are collected by negative pressure generated by a vacuum pump, so that the hand of an operator becomes free; the integrated puncture needle-infusion tube are used for collecting the oocytes and follicular fluid so as to avoid damage to surface granular cells of the oocytes during long-distance movement and to enhance collection speed, so that residence time for the oocytes in an external environment is reduced to be shortest; and by adopting the hand-held collection tube, the influence of environment temperature on the temperatures of the oocytes and the follicular fluid is reduced to be smallest, and the temperatures of the oocytes and the follicular fluid are stabilized at a human body surface temperature, thereby imitating the temperature of an animal and reducing damage of ultraviolet rays in outside light on a sample in the collection tube.

Description

Extract the oocyte device fast
Technical field
This utility model relates to a kind of quick extraction oocyte system, is suitable for the collection of oocyte on the female animal ovary.
Background technology
At present, the method for oocyte collection peel off method, puncture method, blade cuts method, syringe pump method and vacuum pump acquisition method.In the concrete operations of existing collection female animal oocyte, peel off method, puncture method, these three kinds of method complex operations of blade cuts method, length consuming time, the oocyte of acquisition is few.And the picker when using the syringe pump method one commonly used hold ovary; The another hands is taken syringe; The negative pressure that adopts syringe to produce is gathered the follicle on ovary surface, and oocyte and follicular fluid are collected in the syringe together, again collecting to such an extent that mixture is shifted onto from syringe and picked up in the ovum ware; Job step is many, and is consuming time also many.Existing vacuum pump acquisition method is with U.S. Patent Publication US2004/0092791 A1, and be example open May 13 2004 date, and it has very long one section tube for transfusion between puncture needle and collection tube.To cause ova nuda occurring too much because oocyte advances in this long tube for transfusion.Get many, the consuming time length of job step and the many deficiencies of existing vacuum pump acquisition method ova nuda of ovum in order to overcome existing acquisition method at every turn; This patent provides a kind of quick extraction oocyte device; This installs, and not only job step is few; And weak point easy and simple to handle, consuming time, the ratio that ova nuda appears in what is more important is few.
Summary of the invention
This utility model problem to be solved be to answer market urgently, overcome the deficiency of prior art, a kind of a kind of quick extraction oocyte device easy and simple to handle is provided.
The technical scheme of this utility model is: a kind of quick extraction oocyte device; Comprise collection tube, pipe close, puncture needle and airway; Tube for transfusion between collection tube and puncture needle passes pipe close; It is characterized in that: tube for transfusion and puncture needle are one, and the length of tube for transfusion is 3-5cm, and tube for transfusion and puncture needle are in line or at angle.
Through the negative pressure oocyte collection that vacuum pump produces, the hands of having liberated the operator; Integral type puncture needle-tube for transfusion collection oocyte and follicular fluid avoided long distance motion to cause oocyte surface particles cells injury, and improved the speed of gathering, and the time decreased that oocyte is in the external environment arrives minimum; The hand held collection tube, the influence that guarantees the suffered ambient temperature of temperature of oocyte and follicular fluid drops to minimum, is stabilized in the human surface temperature, has simulated the body temperature of animal, and has reduced ultraviolet in the extraneous light to the damage of sample in the collection tube.
Aforesaid quick extraction oocyte device, it is characterized in that: the length of tube for transfusion in collection tube is longer than the length of airway in collection tube slightly.In the time of like this can liquid level approaches the tube for transfusion mouth of pipe in collection tube, still can not allow liquid to go in the airway and go.
Aforesaid quick extraction oocyte device is characterized in that: adopt above-mentioned two device series connection to use, the airway of preceding harvester is connected with connection tube with the puncture needle of back protector.Back one device makes the oocyte and the follicular fluid that collect be unlikely to be drawn onto in the vacuum pump as the protectiveness test tube.
A kind of quick extraction oocyte device that provides according to this utility model connects airway at the air inlet of vacuum pump, with one or with two placed in-line forms of device, oocyte collection.The pipe that imports in the oocyte collection tube and derive is all shorter, and only outstanding cover bottom a bit; The negative pressure that produces through vacuum pump arrives puncture needle again through protecting tube protection arrival oocyte collection tube, and when the lancet puncture ovary, the oocyte complex can be sucked the oocyte collection tube by negative pressure.1, after integral type puncture needle-tube for transfusion thrusts ovary; Collect oocyte and follicular fluid together in the oocyte collection tube through short tube for transfusion, short tube for transfusion avoids long distance motion to cause oocyte surface particles cells injury, and has improved the speed of gathering; The time decreased that oocyte is in the external environment arrives minimum; 2, hand held collection tube, the influence of the suffered ambient temperature of temperature of assurance oocyte and follicular fluid drops to minimum, is stabilized in the human surface temperature; Simulated the body temperature of animal; And reduced ultraviolet in the extraneous light to the damage of sample in the collection tube, 3, be in series with a protectiveness test tube (identical) with collection tube, can guarantee that the oocyte and the follicular fluid that collect are unlikely to be drawn onto in the vacuum pump.
The specific embodiment of this utility model is provided by following examples and accompanying drawing thereof in detail.
Description of drawings
The structural representation of Fig. 1 documents U.S. Pat 2004/0092791 A1.
Fig. 2 is the structural representation of this utility model embodiment.
Fig. 3 is the structural representation of embodiment 2.
The specific embodiment
The explanation of labelling in Fig. 2/3: puncture needle 1, tube for transfusion 2, pipe close 3, collection tube 4, airway 5, connection tube 6, harvester 7, protector 8.
Below in conjunction with accompanying drawing and preferred embodiment, to the specific embodiment, structure, characteristic and the effect thereof that provides according to this utility model.
A kind of quick extraction oocyte device; Comprise collection tube 4, pipe close 3, puncture needle 1 and airway 5; The tube for transfusion 2 that collection tube 4 and puncture needle are 1 passes pipe close 3; It is characterized in that: tube for transfusion 2 is one with puncture needle 1, and the length of tube for transfusion is 3-5cm, and tube for transfusion and puncture needle are in line or at angle.In the present embodiment, tube for transfusion and puncture needle meet at right angles, and tube for transfusion is 4CM, outside the pipe close 3 2cm are arranged, and in pipe close He in the collection tube 4,2cm are arranged.
The length of tube for transfusion 2 in collection tube 4 is longer than the length of airway 5 in collection tube slightly in the present embodiment.In the time of like this can liquid level approaches the tube for transfusion mouth of pipe in collection tube, still can not allow liquid to go in the airway and go.
Referring to Fig. 3 this utility model is embodiment 2.Adopt above-mentioned two device series connection to use, the airway of preceding harvester 7 is connected with silica gel connection tube 6 with the puncture needle of back protector 8.Last device is as collection tube, and back one device makes the oocyte and the follicular fluid that collect be unlikely to be drawn onto in the vacuum pump as the protectiveness test tube.
Various assembly room temperature is dry down, and autoclave sterilization according to the explanation assembling, can use.During use, grasp collection tube, make the syringe needle of puncture needle thrust ovary, oocyte is blended in the follicular fluid together by sucking-off, through directly getting into collection tube behind more short-range puncture needle.Operator's watch face temperature stabilization is about 35 degree, and collecting pipe has only 7 centimetres high, equates with common people's palm width, is held in the hand not use water-bath just can be incubated, and avoids low temperature that the low temperature of oocyte is hit.Between collection tube and vacuum pump, add a device again as protecting tube (structure is identical with last collection tube), can avoid that follicular fluid is drawn onto in the vacuum pump more for a long time in the collection tube in the hands, thereby protected experiment equipment.
The above; It only is the preferred embodiment of this utility model; Be not that this utility model is done any pro forma restriction; Every technical spirit according to this utility model all still belongs in the scope of this utility model technical scheme any simple modification, equivalent variations and modification that above embodiment did.

Claims (3)

1. one kind is extracted the oocyte device fast, comprises collection tube, pipe close; Puncture needle and airway, the tube for transfusion between collection tube and puncture needle passes pipe close, it is characterized in that: tube for transfusion and puncture needle are one; The length of tube for transfusion is 3-5cm, and tube for transfusion and puncture needle are in line or at angle.
2. a kind of quick extraction oocyte device according to claim 1, it is characterized in that: the length of tube for transfusion in collection tube is longer than the length of airway in collection tube slightly.
3. a kind of quick extraction oocyte device according to claim 1 and 2 is characterized in that: adopt above-mentioned two device series connection to use, the airway of preceding harvester is connected with connection tube with the puncture needle of back protector.
CN2011203141658U 2011-08-26 2011-08-26 Device for extracting oocytes quickly Expired - Fee Related CN202313662U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN2011203141658U CN202313662U (en) 2011-08-26 2011-08-26 Device for extracting oocytes quickly

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN2011203141658U CN202313662U (en) 2011-08-26 2011-08-26 Device for extracting oocytes quickly

Publications (1)

Publication Number Publication Date
CN202313662U true CN202313662U (en) 2012-07-11

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Application Number Title Priority Date Filing Date
CN2011203141658U Expired - Fee Related CN202313662U (en) 2011-08-26 2011-08-26 Device for extracting oocytes quickly

Country Status (1)

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CN (1) CN202313662U (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108504554A (en) * 2018-05-24 2018-09-07 中国农业科学院兰州畜牧与兽药研究所 A kind of outer ovarian follicle collection of animal body
CN108618834A (en) * 2017-03-22 2018-10-09 上海康德莱企业发展集团股份有限公司 A kind of heat preservation oocyte collector
CN113180035A (en) * 2021-04-27 2021-07-30 沭阳县人民医院 Can draw save conveyer of oocyte fast

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108618834A (en) * 2017-03-22 2018-10-09 上海康德莱企业发展集团股份有限公司 A kind of heat preservation oocyte collector
CN108504554A (en) * 2018-05-24 2018-09-07 中国农业科学院兰州畜牧与兽药研究所 A kind of outer ovarian follicle collection of animal body
CN113180035A (en) * 2021-04-27 2021-07-30 沭阳县人民医院 Can draw save conveyer of oocyte fast

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C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20120711

Termination date: 20140826

EXPY Termination of patent right or utility model