CN102120111B - Single-filtration seminal fluid separating centrifuge tube - Google Patents
Single-filtration seminal fluid separating centrifuge tube Download PDFInfo
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- CN102120111B CN102120111B CN2011100312940A CN201110031294A CN102120111B CN 102120111 B CN102120111 B CN 102120111B CN 2011100312940 A CN2011100312940 A CN 2011100312940A CN 201110031294 A CN201110031294 A CN 201110031294A CN 102120111 B CN102120111 B CN 102120111B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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Abstract
The invention discloses a single-filtration seminal fluid separating centrifuge tube, which comprises a sleeve, wherein a filtering pipe is arranged in the sleeve; the outer wall of the upper part of the sleeve has threads; the upper part of the filtering pipe is cylindrical and the lower part of the filtering pipe is shaped like a truncated cone; the bottom end of the filtering pipe is connected with a conduit with a diameter smaller than that of the filtering pipe; the filtering pipe is communicated with the conduit; a buffering net is arranged on the upper end of the truncated cone of the filter pipe; the outer wall of the buffering net is in tight fit with the inner wall of the upper end of the truncated cone; upper, middle and lower three layers of polyester fiber filtering membranes are arranged between the buffering net and a supporting net frame; through holes are formed on the filtering membranes uniformly; and a first supporting ring, a second supporting ring and a third supporting ring are matched with the inner wall of the truncated cone respectively. In the invention, a purely physical method is used to filter a seminal fluid sample which has a liquefaction abnormal, so fibrin filaments, gel particles and cell components in the seminal fluid are removed, the seminal fluid is completely liquefied, the sperms move freely, and part of sperms with special shapes and part of dead sperms can be removed at the same time.
Description
Technical field
The present invention relates to the seminal fluid separator, is that a kind of disposable filtering formula seminal fluid separates centrifuge tube.
Background technology
Nonliquefaction of semen can hinder sperm and move about, and causes sterility the most at last.Owing to causing the male sex of sterility, nonliquefaction of semen accounts for the 7%-8% of sterility.At present generally adopt the auxiliary procreation technology effect better for the treatment of these patients with infertility.Yet; The important prerequisite of auxiliary procreation technology is how from fresh seminal fluid, to obtain the sperm of a large amount of high-qualitys; There is difficult optimum problem again in the semen sample of abnormal liquefaction external at present, even there is abundant high-quality sperm also difficult with its effective separation, for this reason; Those skilled in the art increase enzyme preparation in the semen sample of abnormal liquefaction, go ins's art again through washing after short its liquefaction.Though this method is simple to operation,, the seminal fluid pH value changes after the adding enzyme preparation in seminal fluid; Be prone to make sperm survival rate and vigor to descend, to the sperm structure particularly sperm membrane cause damage, cause pregnancy rate lower; Simultaneously, how many difficult controls that add enzyme preparation amount.In order to address the above problem, improve pregnancy rate, in recent years, those skilled in the art were devoted to study the seminal fluid that adopts pure physical method for separation abnormal liquefaction; Separate the purpose that obtains the high-quality sperm to reach, some device of having reported is though be used for separating the sperm of seminal fluid; But, because purposes is different, its complex structure; The separation process complicated operation influences the motility rate of sperm, and pregnancy rate is still difficult to be improved.
Summary of the invention
The purpose of this invention is to provide a kind of disposable filtering formula seminal fluid and separate centrifuge tube; It adopts pure physical method that the semen sample of abnormal liquefaction is filtered; Not only can remove fiber protein yarn, gel particle, cell component in the seminal fluid; Seminal fluid is liquefied fully, make sperm free movable, go back part that can be removed paramophia sperm and part Necrospermia simultaneously.
The present invention is for realizing above-mentioned purpose, and realize through following technical scheme: disposable filtering formula seminal fluid separates centrifuge tube, comprises sleeve pipe; Chimney filter is installed in the sleeve pipe, on the sleeve pipe external wall of upper portion screw thread is set, chimney filter top is cylindric; The bottom is truncated cone shape, the bottom connecting duct of chimney filter, and conduit diameter is less than the diameter of chimney filter; Chimney filter communicates with conduit, in the chimney filter truncated cone upper end buffering net is installed, and the inwall of the outer wall of buffering net and truncated cone upper end closely cooperates; The lower end of the truncated cone is installed and is supported rack, and the outer wall that supports rack closely cooperates with the inwall of truncated cone lower end, at the buffering net and support install between the rack under three strata ester fiber filter membranes; Be uniformly distributed with through hole on the filter membrane, the through-hole diameter on the filter membrane of upper strata is 32-36 μ m, and the through-hole diameter on the filter membrane of middle level is 9-12 μ m; Through-hole diameter on lower floor's filter membrane is 5-7 μ m, and the distance between upper strata filter membrane and middle level filter membrane is 0.6-1.2mm, and the distance between middle level filter membrane and lower floor's filter membrane is 0.5-0.7mm; The edge of upper strata filter membrane is connected with first support ring; The edge of middle level filter membrane is connected with second support ring, and the edge of lower floor's filter membrane is connected with three-support ring, and the outer wall of first support ring, second support ring and three-support ring matches with the inwall of the truncated cone respectively.Buffering evenly is provided with square net on the net, and the net loop diameter of buffering net periphery is 0.6-1mm, and the dowel diameter between grid is 0.08-0.15mm, and the spacing of each grid is 0.15-0.2mm.The end face of chimney filter is higher than the end face of sleeve pipe, and chimney filter outer wall top is provided with external screw thread, and internal surface of sleeve pipe top is provided with internal thread, and chimney filter adopts with sleeve pipe and is threaded.The diameter of first support ring, second support ring and three-support ring is 0.6-1mm, and the diameter of upper strata filter membrane, middle level filter membrane and lower floor's filter membrane is all than the big 0.6-1.2mm of diameter of support ring.
The invention has the advantages that: it has accomplished the seminal fluid of pure physical method for separation abnormal liquefaction through structure according to the invention; Not only can remove fiber protein yarn, gel particle, cell component in the seminal fluid; Seminal fluid is liquefied fully, make sperm free movable, go back part that can be removed paramophia sperm and part Necrospermia simultaneously; Make sperm no any damage in separation process; Seminal fluid liquefied fraction after the filtration is 100%, and sperm motility rate and vigor, normal morphology sperm percentage, the motile sperm rate of recovery are significantly increased, and the centrifugal back sperm sum rate of recovery improves 43.7% than simple the filtration; Therefore device of the present invention is equally applicable to the patient of lack of quantity of spermatic fluid; Can not produce because of lack of quantity of spermatic fluid and can't separate or the phenomenon of inferior separating effect etc., the present invention also has whole separation process and does not have any pollution, simple to operate, and advantages such as cross-infection are avoided in disposable use.
Description of drawings
Accompanying drawing 1 is a structural representation of the present invention, and accompanying drawing 2 is I portion structure for amplifying sketch mapes in the accompanying drawing 1; Accompanying drawing 3 is structural representations of buffering net 11; Accompanying drawing 4 is II portion structure for amplifying sketch mapes in the accompanying drawing 2.
The specific embodiment
The contrast accompanying drawing further specifies the present invention.
1 is sleeve pipe among the figure, and chimney filter 3 is installed in the sleeve pipe 1, on sleeve pipe 1 external wall of upper portion screw thread is set; Sleeve pipe 1 is threaded with capping 2, and chimney filter 3 tops are cylindric, and the bottom is the truncated cone 4 shapes; The bottom connecting duct 5 of chimney filter 3, conduit 5 diameters are less than the diameter of chimney filter 3, and chimney filter 3 communicates with conduit 5; Buffering net 11 is installed in chimney filter 3 truncateds cone 4 upper ends, and the outer wall of buffering net 11 and the inwall of the truncated cone 4 upper ends closely cooperate, and the lower end of the truncated cone 4 is installed and supported rack 15; The outer wall that supports rack 15 closely cooperates with the inwall of the truncated cone 4 lower ends, at buffering net 11 and support install between the rack 15 under three strata ester fiber filter membranes, be uniformly distributed with through hole on the filter membrane; Through-hole diameter on the upper strata filter membrane 12 is 32-36 μ m, and the through-hole diameter on the middle level filter membrane 13 is 9-12 μ m, and the through-hole diameter on lower floor's filter membrane 14 is 5-7 μ m; The distance that upper strata filter membrane 12 and middle level filter membrane are 13 is 0.6-1.2mm; The distance that middle level filter membrane 13 and lower floor's filter membrane are 14 is 0.5-0.7mm, and the edge of upper strata filter membrane 12 is connected with first support ring 7, and the edge of middle level filter membrane 13 is connected with second support ring 8; The edge of lower floor's filter membrane 14 is connected with three-support ring 9, and the outer wall of first support ring 7, second support ring 8 and three-support ring 9 matches with the inwall of the truncated cone 4 respectively.Such scheme can be realized the object of the invention, and can reach advantage according to the invention.
The further scheme of the present invention is; On the buffering net 11 described in apparatus of the present invention square net is set evenly; It is 0.6-1mm that the net of buffering net 11 peripheries encloses 6 diameters; Dowel diameter between grid is 0.08-0.15mm, and the spacing of each grid is 0.15-0.2mm, and this scheme further makes the seminal fluid of abnormal liquefaction can slowly, evenly get into the upper strata filter membrane.
The end face of the chimney filter 3 described in apparatus of the present invention is higher than the end face of sleeve pipe 1, and chimney filter 3 outer wall tops are provided with external screw thread, and sleeve pipe 1 upper inside wall is provided with internal thread, and chimney filter 3 adopts with sleeve pipe 1 and is threaded, and chimney filter 3 also can adopt interference fit to be connected with sleeve pipe 1.
The preferred scheme of syndeton between described filter membrane of apparatus of the present invention and support ring is; The diameter of described first support ring 7, second support ring 8 and three-support ring 9 is 0.6-1mm; The diameter of upper strata filter membrane 12, middle level filter membrane 13 and lower floor's filter membrane 14 is all than the big 0.6-1.2mm of diameter of support ring; Make radius that each layer filter membrane coat support ring separately with upper surface, and combine closely structure as shown in Figure 4 with the truncated cone 4 inwalls; The syndeton of filter membrane shown in the figure and support ring is simple, easy to operate, does not have any pollution.Certainly, be connected multiple structures such as also can adopting hot melt, groove chucking method between filter membrane and support ring, still, these structures exist complex structure, manufacturing cost high, are prone to produce deficiencies such as pollution.
Sleeve pipe 1 in apparatus of the present invention, chimney filter 3, conduit 5, capping 2, buffering net 11, supporting network 15, first support ring 7, second support ring 8 and three-support ring 9 all adopt the medical PVC material; Disposable use after filter membrane and above-mentioned parts are all sterilized; Apparatus of the present invention are packed separately, destroy at once after the disposable use.
Apparatus of the present invention in use; The seminal fluid of abnormal liquefaction evenly is poured on the buffering net 11 in the chimney filter 3, puts into centrifuge with the centrifugal 5min of 2500r/min, the seminal fluid that makes abnormal liquefaction is uniformly through upper strata filter membrane 12; With fiber protein yarn in the seminal fluid and gel particle filtering; Again through the cell component in the middle level filter membrane 13 filtering seminal fluid, at last through behind lower floor's filter membrane 14 with part paramophia sperm and the filtering of part Necrospermia, the seminal fluid that obtains at last gets into the bottom of sleeve pipe 1.Through detecting, this moment, seminal fluid was liquefied fully, and sperm freely moves about, and liquefied fraction is 100%.
10 is the net circles that support on the rack 15 among the figure.
Claims (2)
1. disposable filtering formula seminal fluid separates centrifuge tube, it is characterized in that: comprise sleeve pipe (1), chimney filter (3) is installed in the sleeve pipe (1); On sleeve pipe (1) external wall of upper portion screw thread is set, chimney filter (3) top is cylindric, and the bottom is the truncated cone (4) shape; The bottom connecting duct (5) of chimney filter (3); Conduit (5) diameter is less than the diameter of chimney filter (3), and chimney filter (3) communicates with conduit (5), in chimney filter (3) truncated cone (4) upper end buffering net (11) is installed; The inwall of the outer wall of buffering net (11) and the truncated cone (4) upper end closely cooperates; The lower end of the truncated cone (4) is installed and is supported rack (15), and the outer wall that supports rack (15) closely cooperates with the inwall of the truncated cone (4) lower end, between buffering net (11) and support rack (15), installing under three strata ester fiber filter membranes; Be uniformly distributed with through hole on the filter membrane; Through-hole diameter on the upper strata filter membrane (12) is 32-36 μ m, and the through-hole diameter on the middle level filter membrane (13) is 9-12 μ m, and the through-hole diameter on lower floor's filter membrane (14) is 5-7 μ m; Distance between upper strata filter membrane (12) and middle level filter membrane (13) is 0.6-1.2mm; Distance between middle level filter membrane (13) and lower floor's filter membrane (14) is 0.5-0.7mm, and the edge of upper strata filter membrane (12) is connected with first support ring (7), and the edge of middle level filter membrane (13) is connected with second support ring (8); The edge of lower floor's filter membrane (14) is connected with three-support ring (9), and the outer wall of first support ring (7), second support ring (8) and three-support ring (9) matches with the inwall of the truncated cone (4) respectively.
2. disposable filtering formula seminal fluid according to claim 1 separates centrifuge tube; It is characterized in that: the end face of chimney filter (3) is higher than the end face of sleeve pipe (1); Chimney filter (3) outer wall top is provided with external screw thread; Sleeve pipe (1) upper inside wall is provided with internal thread, and chimney filter (3) adopts with sleeve pipe (1) and is threaded.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100312940A CN102120111B (en) | 2011-01-28 | 2011-01-28 | Single-filtration seminal fluid separating centrifuge tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100312940A CN102120111B (en) | 2011-01-28 | 2011-01-28 | Single-filtration seminal fluid separating centrifuge tube |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102120111A CN102120111A (en) | 2011-07-13 |
| CN102120111B true CN102120111B (en) | 2012-10-31 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011100312940A Expired - Fee Related CN102120111B (en) | 2011-01-28 | 2011-01-28 | Single-filtration seminal fluid separating centrifuge tube |
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| EP2801610B1 (en) | 2012-01-04 | 2019-10-02 | Japan Tissue Engineering Co., Ltd. | Cell separation container |
| EP2664367A1 (en) | 2012-05-15 | 2013-11-20 | Miltenyi Biotec GmbH | Stackable cell strainer |
| CN103923832B (en) * | 2014-03-27 | 2015-09-09 | 安徽农业大学 | A kind of cell detachment device and cell method for splitting |
| CN104498346A (en) * | 2014-12-12 | 2015-04-08 | 张群 | Micron-hole-based apparatus for rapidly screening and collecting healthy sperms and application method of micron-hole-based apparatus |
| CN104651224A (en) * | 2015-02-10 | 2015-05-27 | 张丽红 | Double-tube vacuum film sperm separation method and separation device |
| CN105985903A (en) * | 2015-02-12 | 2016-10-05 | 谭巍 | Cell collecting device and application thereof |
| CN105116152B (en) * | 2015-08-10 | 2017-10-03 | 中国人民解放军第二军医大学 | A kind of ELISA detection kit and detection method of humanized's urine Endocan albumen |
| CN108717111B (en) * | 2018-04-24 | 2023-11-10 | 金华市人民医院 | Full-automatic semen detection device and method |
Citations (4)
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|---|---|---|---|---|
| CN2686638Y (en) * | 2004-01-19 | 2005-03-23 | 齐中祥 | Semen optimizing bube |
| CN2790415Y (en) * | 2005-04-13 | 2006-06-28 | 阎沈军 | Filter type sperm separator |
| CN2824865Y (en) * | 2005-05-02 | 2006-10-11 | 尼普洛株式会社 | A kit incorporating sperm concentration clean tube and hold unit thereof |
| CN101805691A (en) * | 2010-04-30 | 2010-08-18 | 邱毅 | Inner sleeve type separation screening sperm tube |
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2011
- 2011-01-28 CN CN2011100312940A patent/CN102120111B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2686638Y (en) * | 2004-01-19 | 2005-03-23 | 齐中祥 | Semen optimizing bube |
| CN2790415Y (en) * | 2005-04-13 | 2006-06-28 | 阎沈军 | Filter type sperm separator |
| CN2824865Y (en) * | 2005-05-02 | 2006-10-11 | 尼普洛株式会社 | A kit incorporating sperm concentration clean tube and hold unit thereof |
| CN101805691A (en) * | 2010-04-30 | 2010-08-18 | 邱毅 | Inner sleeve type separation screening sperm tube |
Non-Patent Citations (2)
| Title |
|---|
| 李志凌 等.辅助生殖技术中的精液优选处理.《生殖医学杂志》.2007,第16卷(第3期), * |
| 李蜀婧 等.少、弱精子症的精子优选技术.《中国优生与遗传杂志》.2006,第14卷(第9期), * |
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| CN102120111A (en) | 2011-07-13 |
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