CN105985903A - Cell collecting device and application thereof - Google Patents
Cell collecting device and application thereof Download PDFInfo
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- CN105985903A CN105985903A CN201510075690.1A CN201510075690A CN105985903A CN 105985903 A CN105985903 A CN 105985903A CN 201510075690 A CN201510075690 A CN 201510075690A CN 105985903 A CN105985903 A CN 105985903A
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- filter
- cell
- collection device
- cell collection
- filter membrane
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Abstract
The invention provides a cell collecting device and application thereof. The cell collecting device comprises a filter liquor collecting bottle and a filter arranged on the top of the filter liquor collecting bottle, wherein the filter consists of a sample containing container, a filtering device and a piston device; the filtering device is arranged at the lower part of the container; the piston device extends into the container; and a pressure device is arranged at the upper part of the filter. The cell collecting device provided by the invention has the advantages that the structure is simple; the cost is low; the cell collecting device is suitable for being popularized in rural areas; through the use of the device, the operation of the device is simple; the speed is high; the work efficiency is improved; the work intensity is reduced; the examination positive rate is improved; and the cost is reduced.
Description
Technical field
The present invention relates to field of medical device, particularly relate to a kind of cell collection device and application thereof.
Background technology
One of forward position of current early diagnosis of cancer and monitoring development is how to utilize blood, urine or other bodies
Liquid carries out cancer diagnosis and examination.The advantage of this technology maximum is: noninvasive detects, can be in cancer
Occur in early days efficiently diagnosis cancer and can Reusability in a short time, thus realize immediately monitoring.It addition,
It avoids patient to accept unnecessary biopsy, and testing cost is the most relatively cheap, is conducive to clinically
Extensively application.Along with the development of science and technology, the accuracy of this detection technique and sensitivity in recent years is the most significantly
Improve.But, how the cancerous cell of extremely trace from a large amount of body fluid easy, separate quickly and be
Current research person endeavours problem to be solved.
Use traditional centrifugal method in the past and collect cell.The cell that need to collect is put into test tube,
Conventional centrifugal, but find some shortcomings through long-term clinical practice: embody a concentrated reflection of after test tube is centrifugal, go up
The when of clear, the uncleanest, and need to take back and forth.Such result obtains after making by centrifugation
To the cell of fully enrichment, during taking repeatedly, again with the liquid of remaining (because supernatant is by manually grasping
Make, it is impossible to fall the cleanest) it is mutually mixed, and make the cell concentrated by centrifugation be watered down once again.Every time
Removing supernatant, all can lose cell, cause the cell quantity finally given to greatly reduce, reducing sample has
Effect property and positive clinical rate, and it is greatly increased the working strength of experimenter.And, this method cannot
Separate different types of cell.
In view of this, a kind of appearance of cell harvestor easy and simple to handle, that reduce labor intensity is the most necessary
?.
Summary of the invention
The present invention proposes a kind of cell collection device, solve in prior art cannot quickly, easy collect carefully
The problem of born of the same parents.
The technical scheme is that and be achieved in that:
A kind of cell collection device, including filtrate collection bottle and the mistake that is arranged on described filtrate collection bottle top
Filter；
Described filter is formed by holding the container of sample, defecator and piston apparatus；
Described defecator is arranged on the bottom of described container；Described piston apparatus stretches into the inside of described container；
It is provided with pressure apparatus on the top of described filter.
As preferred technical scheme, described filter is syringe filter.
As preferred technical scheme, described pressure apparatus is elastic band, and described elastic band both sides are fixed respectively
In described filtrate collection bottle both sides, the middle part of described elastic band is placed in described piston apparatus top.
As preferred technical scheme, described defecator is cone or wedge shape.
As preferred technical scheme, described defecator includes support, filter membrane and cell catch tray, described
Filter membrane fits in the inside of support, and described cell catch tray is arranged on the bottom of support.
As preferred technical scheme, described filter membrane is 5uM filter membrane or 2uM filter membrane.
As preferred technical scheme, between described piston apparatus and sample plane, it is provided with air tap.Its hollow
The capacity of aeroembolism is about 8-15ml；This air tap can set other volumes according to demand；The size of device
Can be according to sample size, the viscosity of body fluid and the number change containing cell.
As preferred technical scheme, the inside of described container is provided with sealing device.
A kind of method using above-mentioned cell collection device, including:
Place sample in filter；
Piston apparatus is put in filter；Air tap is formed between piston apparatus and sample；
Filtrate collection bottle is placed in the bottom of filter；
Forces piston moves downward, and makes whole sample and major part air tap filter filter membrane, collects filtrate and get final product
Required detection product.
Technique scheme of the present invention, obtains relative to prior art and provides the benefit that:
(1) this apparatus structure is simple, with low cost, is suitable for popularizing in rural area；
(2) from the use of this device, this device is simple to operate, and speed is fast, improves work efficiency,
Reduce labor intensity；And improve inspection positive rate, reduce cost.
Accompanying drawing explanation
In order to be illustrated more clearly that embodiment of the present invention or technical scheme of the prior art, below will be to reality
Execute the required accompanying drawing used in scheme or description of the prior art to be briefly described, it should be apparent that below,
Accompanying drawing in description is only some embodiments of the present invention, for those of ordinary skill in the art,
On the premise of not paying creative work, it is also possible to obtain other accompanying drawing according to these accompanying drawings.
Fig. 1 is the structural representation of the present invention a kind of cell mobile device；
Wherein:
1-cell collection device, 11-filtrate collection bottle, 12-filter, 13-pressure apparatus, 121-container, 122-
Defecator, 123-piston apparatus, 124-air tap, 1221-support, 1222-filter membrane, 1223-cell are collected
Dish.
Detailed description of the invention
Technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that retouched
The embodiment stated is only a part of embodiment of the present invention rather than whole embodiments.Based in the present invention
Embodiment, those of ordinary skill in the art obtained under not making creative work premise all its
His embodiment, broadly falls into the scope of protection of the invention.
The principle of the present invention is: the method using physical solution to retain design and chemical surface treatment, improves
The efficiency of a small amount of cell in capture solution.
As shown in Figure 1: cell collection device 1, including filtrate collection bottle 11 and filter 12.Filter 12
It is arranged on the top of filtrate collection bottle 11.Its filter 12 is by holding the container 121 of sample, defecator
122 and piston apparatus 123 form, wherein defecator 122 is arranged on the bottom of container 121；Piston fills
Putting 123 is the inside stretching into container 121 when specifically used, so the bottom of piston apparatus 123 is to arrange
In the inside of container 121, the top of piston 123 is positioned at the outside of container 121, and piston 123 is inapplicable
When in the outside of container 121, there is no annexation when this is the most inapplicable, be independent monomer.
Container 121 is mainly used to place the solution examples such as urine.Filter 12 in the present embodiment selects syringe to filter
Device, so defecator 122 is cone or wedge shape；This defecator 122 includes support 1221, filter membrane
1222 and cell catch tray 1223, filter membrane 1222 fits in the inside of support 1221, cell catch tray 1223
Being arranged on the bottom of support 1221, filter membrane 1222 therein selects according to the demand of filtered sample, this
In embodiment, filtered sample is urine, so the filter membrane 1222 chosen is 5uM filter membrane, other in urine are thin
Born of the same parents' (including erythrocyte, leukocyte) with filter liquor by the filter membrane 1222 of 5uM, and prostate epithelial cell
(diameter 15-40uM) is big, so filter membrane cannot be passed through, and is enriched on catch tray.When the aperture of filter membrane is 2uM
Time, all cells all cannot pass through filter membrane, and is enriched on catch tray.Namely 5uM filter membrane is used for retaining
Skin or cancerous cell；And 2uM filter membrane is used for retaining all cells.Set between piston apparatus 123 and sample plane
Having air tap 124, wherein the capacity of air tap is about 10ml；This air tap can set it according to demand
His volume；The size of device can be according to sample size, the viscosity of body fluid and the number change containing cell.For
Promotion piston apparatus 123 moves, and is provided with pressure apparatus 13, wherein pressure apparatus on the top of filter 12
13 can also select according to the custom of those skilled in the art, the pressure apparatus 13 selected in the present embodiment
For elastic band, the both sides of elastic band 13 are separately fixed at filtrate collection bottle 11 both sides, and the middle part of elastic band is put
In piston apparatus 123 top, when pressure apparatus 13 forces piston moves downward, whole urine samples are with big
Partial air bolt 124, filters filter membrane, only a small amount of liquid, stays in cell catch tray 1223.In order to more preferably
Filtration, be additionally provided with sealing device in the inside of container 121, i.e. sealing ring.
The using method of above-mentioned cell collection device be described below:
The work of this device is described as a example by separating prostate epithelial cell (including cancerous cell) from urine
Process.
50ml urine sample is added in syringe filter, wherein granule less than 10uM of urine, diameter and
The filter membrane that hemocyte will pass through with urine, enters filtrate collection bottle 11.Piston is inserted on syringe filter top
Device 123, forms the air tap of one section of about 10ml between urine and piston apparatus 123.Filter will be fixed on
The elastic band of liquid receiving flask 11 both sides, is placed in piston apparatus 123 top.The elasticity of elastic band is by forces piston
Device 123 moves downward, and makes whole urine sample and major part air tap, filters filter membrane 1222, the most on a small quantity
Liquid, stays in catch tray 1223.In this process, prostate epithelial cell (diameter 15-40uM) ratio
((including erythrocyte, leukocyte) is big, it is impossible to by the filter membrane of 5uM, cut for other cells in urine
Stay on filter membrane, because gravity is enriched in the catch tray 1223 bottom filter.Catch tray 1223 is hydrophilic, and
And surface homogeneous cross-link antibody.The selection of antibody is determined by the antigen expressed by the surface of required collection cell.
Such as, prostate epithelial cell to be separated from urine, catch tray 1223 can cross-link prostate epithelial cell
The antibody of surface antigen PCMA, expresses the prostate epithelial cell of PCMA with affine absorption.Filtered
Cheng Zhong, prostate epithelial cell is by the affine absorption of antibody and is fixed on catch tray 1223.Area 10 squares
The catch tray 1223 of millimeter at most can collect the prostate epithelial cell of 25,000 diameter 20 microns.
After filtration terminates, 20 to 50 microliters of liquid are had to stay on catch tray 1223, with protection absorption at catch tray 1223
On cell, unlikely dehydration.After filtration terminates, loosen elastic band, extract piston apparatus 123 out.In syringe mistake
Filter adds flushing liquor 15ml, repeats filter process, with the urine of eluting residual.Finally, 1ml is added
Flushing liquor, rinses catch tray 1223.Using proboscis suction pipe, sucking-off flushing liquor and the most affine absorption are at catch tray
On other cells.At this moment it is collected in the cell in catch tray 1223 can directly be cracked, extracts RNA.
Above-mentioned filtration and flushing process take around 20 minutes, wherein operating time 5 minutes.Use this device
The cell collected in solution saves 40 minutes than the most conventional method；And collection efficiency and sample can be improved
Quality.
From above-mentioned use process: this device is simple to operate, speed is fast, improves work efficiency, reduces
Labor intensity；And improve inspection positive rate, reduce cost.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all at this
Within bright spirit and principle, any modification, equivalent substitution and improvement etc. made, should be included in this
Within bright protection domain.
Claims (10)
1. a cell collection device, it is characterised in that include filtrate collection bottle and be arranged on described filtrate receipts
The filter at collection bottle top；
Described filter is formed by holding the container of sample, defecator and piston apparatus；
Described defecator is arranged on the bottom of described container；Described piston apparatus stretches into the inside of described container；
It is provided with pressure apparatus on the top of described filter.
A kind of cell collection device the most according to claim 1, it is characterised in that described filter is
Syringe filter.
A kind of cell collection device the most according to claim 1, it is characterised in that described pressure apparatus
For elastic band, described elastic band both sides are separately fixed at described filtrate collection bottle both sides, in described elastic band
Portion is placed in described piston apparatus top.
A kind of cell collection device the most according to claim 1, it is characterised in that described defecator
For cone or wedge shape.
A kind of cell collection device the most according to claim 4, it is characterised in that described defecator
Including support, filter membrane and cell catch tray, described filter membrane fits in the inside of support, described cell catch tray
It is arranged on the bottom of support.
A kind of cell collection device the most according to claim 1, it is characterised in that described filter membrane is 5uM
Filter membrane or 2uM filter membrane.
A kind of cell collection device the most according to claim 1, it is characterised in that described piston apparatus
And it is provided with air tap between sample plane.
A kind of cell collection device the most according to claim 7, it is characterised in that described air tap
Capacity is 8-15ml.
A kind of cell collection device the most according to claim 1, it is characterised in that described container interior
Portion is provided with sealing device.
10. the method using cell collection device described in claim 1-9 any claim, including:
Place sample in filter；
Piston apparatus is put in filter；Air tap is formed between piston apparatus and sample；
Filtrate collection bottle is placed in the bottom of filter；
Forces piston moves downward, and makes whole sample and major part air tap filter filter membrane, collects filtrate and get final product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510075690.1A CN105985903A (en) | 2015-02-12 | 2015-02-12 | Cell collecting device and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510075690.1A CN105985903A (en) | 2015-02-12 | 2015-02-12 | Cell collecting device and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105985903A true CN105985903A (en) | 2016-10-05 |
Family
ID=57042012
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510075690.1A Pending CN105985903A (en) | 2015-02-12 | 2015-02-12 | Cell collecting device and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105985903A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112625863A (en) * | 2020-12-30 | 2021-04-09 | 武汉北度生物科技有限公司 | Cell separator based on extract anti-oxidant active ingredient |
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| CN101544946A (en) * | 2008-03-27 | 2009-09-30 | 财团法人工业技术研究院 | Apparatus and method for cleaning cell, tissue or biological material |
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| CN203196089U (en) * | 2013-05-03 | 2013-09-18 | 李淑珍 | Intravenous injection injector |
| CN203754716U (en) * | 2013-09-30 | 2014-08-06 | 基点认知技术(北京)有限公司 | Cell separation column and cell separation device |
| CN104136126A (en) * | 2011-12-28 | 2014-11-05 | 硅生物系统股份公司 | Devices, apparatus, kit and method for treating a biological sample |
| CN204874521U (en) * | 2015-02-12 | 2015-12-16 | 谭巍 | Cell collection device |
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Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1714292A (en) * | 2002-11-19 | 2005-12-28 | 积水化学工业株式会社 | Plasma or serum separation membrane and filter apparatus including the plasma or serum separation membrane |
| JP3809457B2 (en) * | 2004-05-17 | 2006-08-16 | 積水化学工業株式会社 | Blood separation filter device and vacuum sample collection tube |
| CN101544946A (en) * | 2008-03-27 | 2009-09-30 | 财团法人工业技术研究院 | Apparatus and method for cleaning cell, tissue or biological material |
| CN102120111A (en) * | 2011-01-28 | 2011-07-13 | 山东省计划生育科学技术研究所 | Single-filtration seminal fluid separating centrifuge tube |
| CN104136126A (en) * | 2011-12-28 | 2014-11-05 | 硅生物系统股份公司 | Devices, apparatus, kit and method for treating a biological sample |
| CN203196089U (en) * | 2013-05-03 | 2013-09-18 | 李淑珍 | Intravenous injection injector |
| CN203754716U (en) * | 2013-09-30 | 2014-08-06 | 基点认知技术(北京)有限公司 | Cell separation column and cell separation device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112625863A (en) * | 2020-12-30 | 2021-04-09 | 武汉北度生物科技有限公司 | Cell separator based on extract anti-oxidant active ingredient |
| CN112625863B (en) * | 2020-12-30 | 2022-06-07 | 武汉北度生物科技有限公司 | Cell separator based on extract anti-oxidant active ingredient |
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Application publication date: 20161005 |
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