CN112625863B - Cell separator based on extract anti-oxidant active ingredient - Google Patents

Cell separator based on extract anti-oxidant active ingredient Download PDF

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Publication number
CN112625863B
CN112625863B CN202011620662.0A CN202011620662A CN112625863B CN 112625863 B CN112625863 B CN 112625863B CN 202011620662 A CN202011620662 A CN 202011620662A CN 112625863 B CN112625863 B CN 112625863B
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fixedly connected
tube
cleaning
workbench
separation
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CN112625863A (en
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沈政
程顺巧
江振华
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Wuhan Biobank Biotechnology Co ltd
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Wuhan Biobank Biotechnology Co ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/08Flask, bottle or test tube
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04Filters; Permeable or porous membranes or plates, e.g. dialysis
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E60/00Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
    • Y02E60/10Energy storage using batteries

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  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
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  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a cell separator based on extraction of antioxidant active ingredients, which comprises a workbench, wherein a kit is arranged at the top of the workbench, vertical rods are arranged on the left side and the right side of the top of the workbench, the bottom ends of the two vertical rods are both in contact with the top of the workbench, a separation tube is arranged at the top of the kit, connecting rods are fixedly connected to the left side and the right side of the separation tube, the ends, far away from the two connecting rods, of the two connecting rods are both fixedly connected with a sliding ring, the outer surfaces of the two vertical rods are both in sliding connection with the inner surface of the sliding ring, and a tube head is fixedly connected to the bottom end of the separation tube. This cell separator based on draw anti-oxidant active ingredient drives the clearance board through miniature hydraulic stem and slides, and the clearance board drives clearance sawtooth and cuts the clearance to adnexed unnecessary cell on the filter screen and impurity simultaneously, has avoided the jam of filter screen, and the practicality is stronger.

Description

Cell separator based on extract anti-oxidant active ingredient
Technical Field
The invention relates to the technical field of cell separation, in particular to a cell separator based on extraction of antioxidant active ingredients.
Background
Antioxidation refers to the abbreviation of antioxidant free radicals. The human body continuously generates free radicals in the human body due to continuous contact with the outside, including respiration (oxidation reaction), external pollution, radiation irradiation and other factors. Scientific studies have shown that cancer, aging or other diseases are mostly associated with the production of excess free radicals. Research on antioxidation can effectively overcome the harm caused by the antioxidation, so the antioxidation is listed as one of the main research and development directions by health-care products and cosmetic enterprises, and is also one of the most important functional requirements of the market. Antioxidant is any substance that is present at low concentrations to effectively inhibit the oxidation of free radicals, either by acting directly on the free radicals or by indirectly consuming substances that readily form free radicals, preventing further reactions from occurring. The human body inevitably generates free radicals and also naturally generates antioxidant substances resisting the free radicals so as to counteract the oxidative attack of the free radicals on human cells. Research proves that the antioxidant system of the human body is a system which is comparable to the immune system and has perfect and complex functions, and the stronger the antioxidant capacity of the body, the healthier the body is, and the longer the life is. The antioxidant substances of the human body are synthesized by themselves and are also supplied by food. Enzymatic and non-enzymatic antioxidants play a crucial role in protecting peroxidative damage due to exercise. The supplement of the plant active selenium for enhancing the antioxidation is beneficial to reducing the generation of free radicals or accelerating the elimination of the free radicals by the sports organism so as to resist the side effect of the free radicals, thereby being beneficial to the health of general people and athletes, possibly delaying the occurrence of sports fatigue and accelerating the physical recovery, and the effect of taking the antioxidant by physical exercisers with large ages is better than that of physical exercisers with small ages.
The mode of current extraction anti-oxidant active ingredient mainly separates the cell that contains oxidation active ingredient through the cell separator, extracts again and tests, but the back separator that finishes extracting receives external bacterial contamination easily to influence the experimental result, can filter unnecessary cell and impurity through setting up the filter screen in the separator in addition, absorb useful cell, but unnecessary cell and impurity adhesion are on the surface of filter screen easily, thereby plug up the filter screen.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a cell separator based on extraction of antioxidant active ingredients, which solves the problems that the existing cell separator is easily polluted by external bacteria, and unnecessary cells and impurities are easily adhered to the surface of a filter screen, so that the filter screen is blocked.
In order to achieve the purpose, the invention is realized by the following technical scheme: a cell separator based on extraction of antioxidant active ingredients comprises a workbench, wherein a kit is arranged at the top of the workbench, vertical rods are arranged on the left side and the right side of the top of the workbench, the bottom ends of the two vertical rods are in contact with the top of the workbench, a separation tube is arranged at the top of the kit, connecting rods are fixedly connected to the left side and the right side of the separation tube, a sliding ring is fixedly connected to one end, away from the two connecting rods, of each connecting rod, the outer surfaces of the two vertical rods are in sliding connection with the inner surface of the sliding ring, a tube head is fixedly connected to the bottom end of the separation tube, and an anti-pollution mechanism is arranged on the surface of the tube head; the anti-pollution mechanism comprises a vertical plate, the top of the vertical plate is fixedly connected with the left side of a tube head, a threaded rod is connected to the left side of the vertical plate in a threaded manner, the right end of the threaded rod penetrates through the vertical plate and extends to the right side of the vertical plate, a rotating block is fixedly connected to the left end of the threaded rod, anti-skid lines are engraved on the surface of the rotating block, a baffle is connected to the right side of the tube head through rotation of a turntable, the bottom of the baffle is in contact with the top of the threaded rod, a connecting block is fixedly connected to the right side of the tube head and located above the turntable, an arc plate is fixedly connected to one end of the connecting block, a rubber plate is fixedly connected to the inner surface of the arc plate, and the size of the arc plate is matched with the size of the baffle; the top sliding connection of separator tube has the slide bar, the inside of separator tube and extension to separator tube is run through to the bottom of slide bar, the bottom fixedly connected with piston of slide bar, the surface of piston and the internal surface sliding connection of separator tube, just be located the below fixedly connected with filter screen of piston between the left and right sides of separator tube inner wall, the left and right sides of separator tube and the equal fixedly connected with in below that is located the connecting rod miniature hydraulic stem, two the looks remote site of miniature hydraulic stem all runs through the separator tube and extends to the inside of separator tube, two the equal fixedly connected with of looks remote site clearance board of miniature hydraulic stem, two the top of clearance board all with the bottom sliding connection of filter screen.
Preferably, the top of the sliding rod is fixedly connected with a handle, and anti-skid lines are carved on the surface of the handle.
Preferably, the two cleaning plates are provided with a plurality of cleaning sawteeth on opposite sides, the cleaning sawteeth are uniformly distributed on the surfaces of the cleaning plates, and the cleaning sawteeth are triangular.
Advantageous effects
The invention provides an automobile charging gun with high safety performance. Compared with the prior art, the method has the following beneficial effects:
(1) the cell separator based on the extraction of the antioxidant active ingredients comprises a vertical plate in an anti-pollution mechanism, the top of the vertical plate is fixedly connected with the left side of a tube head, the left side of the vertical plate is in threaded connection with a threaded rod, the right end of the threaded rod penetrates through the vertical plate and extends to the right side of the vertical plate, the left end of the threaded rod is fixedly connected with a rotating block, anti-skidding lines are engraved on the surface of the rotating block, the right side of the tube head is rotatably connected with a baffle plate through a turntable, the bottom of the baffle plate is in contact with the top of the threaded rod, a connecting block is fixedly connected to the right side of the tube head and positioned above the turntable, one end of the connecting block is fixedly connected with an arc plate, the inner surface of the arc plate is fixedly connected with a rubber plate, the size of the arc plate is matched with the size of the baffle plate, the baffle plate is in contact with the bottom of the tube head through rotating the baffle plate, and then the rotating block is rotated to drive the threaded rod to rotate, and simultaneously slide the threaded rod rightwards, the baffle is finally blocked to prevent the baffle from moving, and the pollution of the tube head by external bacteria after the tube head absorbs cells is avoided by arranging the anti-pollution mechanism, so that the accuracy of the test result is improved;
(2) the cell separator based on the extraction of the antioxidant active ingredients is characterized in that a filter screen is fixedly connected between the left side and the right side of the inner wall of a separation pipe and below a piston, miniature hydraulic rods are fixedly connected between the left side and the right side of the separation pipe and below a connecting rod, opposite ends of the two miniature hydraulic rods penetrate through the separation pipe and extend to the inside of the separation pipe, cleaning plates are fixedly connected with opposite ends of the two miniature hydraulic rods, cleaning sawteeth are arranged on opposite sides of the two cleaning plates, the number of the cleaning sawteeth is multiple, the cleaning plates are uniformly distributed on the surfaces of the cleaning plates, tops of the two cleaning plates are both in sliding connection with bottoms of the filter screen, the bottoms of the filter screen are in sliding connection, the cleaning plates are driven to slide by the miniature hydraulic rods, and meanwhile the cleaning plates drive the cleaning plates to cut and clean unnecessary cells and impurities attached to the filter screen, the blockage of the filter screen is avoided, and the practicability is strong;
(3) this cell separator based on draw anti-oxidant active ingredient, all be provided with the montant through the left and right sides at the workstation top, the bottom of two montants all contacts with the top of workstation, the top of kit is provided with the separator tube, the equal fixedly connected with connecting rod of the left and right sides of separator tube, the equal fixedly connected with slip ring of one end that two connecting rods kept away from mutually, the surface of two montants all with the internal surface sliding connection of slip ring, setting through the slip ring, can make the slip ring slide along the surface of montant, the connecting rod drives the separator tube and slides simultaneously, the montant can be built by a height the separator tube simultaneously, further avoided the separator tube to be contaminated, and also need not the handheld separator tube operation of staff, it is more convenient.
Drawings
FIG. 1 is a front view of the outer structure of the present invention;
FIG. 2 is an enlarged view of a portion of the invention at A in FIG. 1;
FIG. 3 is a top view of a portion of the structure of the present invention;
FIG. 4 is a partial sectional view of the present invention;
fig. 5 is a top view of the filter screen of the present invention.
In the figure: 1-workbench, 2-kit, 3-vertical rod, 4-separation tube, 5-connecting rod, 6-sliding ring, 7-tube head, 8-anti-pollution mechanism, 81-vertical plate, 82-threaded rod, 83-rotating block, 84-rotating disc, 85-baffle, 86-connecting block, 87-arc plate, 88-rubber plate, 9-sliding rod, 10-piston, 11-handle, 12-filter screen, 13-micro hydraulic rod, 14-cleaning plate and 15-cleaning saw tooth.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-5, the present invention provides a technical solution: a cell separator based on extraction of antioxidant active ingredients comprises a workbench 1, a kit 2 is arranged at the top of the workbench 1, cell fluid to be extracted is contained in the kit 2, vertical rods 3 are arranged on the left side and the right side of the top of the workbench 1, the vertical rods 3 can be erected on the top of the workbench 1, the bottom ends of the two vertical rods 3 are in contact with the top of the workbench 1, a separation tube 4 is arranged at the top of the kit 2, the cell fluid can be stored in the separation tube 4, connecting rods 5 are fixedly connected to the left side and the right side of the separation tube 4, a sliding ring 6 is fixedly connected to one end, far away from the two connecting rods 5, of the outer surfaces of the two vertical rods 3 are in sliding connection with the inner surface of the sliding ring 6, a tube head 7 is fixedly connected to the bottom end of the separation tube 4, a through hole is formed in the bottom of the tube head 7 and used for absorbing the cell fluid, and an anti-pollution mechanism 8 is arranged on the surface of the tube head 7; the anti-pollution mechanism 8 comprises a vertical plate 81, the top of the vertical plate 81 is fixedly connected with the left side of the tube head 7, the left side of the vertical plate 81 is in threaded connection with a threaded rod 82, the right end of the threaded rod 82 penetrates through the vertical plate 81 and extends to the right side of the vertical plate 81, the left end of the threaded rod 82 is fixedly connected with a rotating block 83, anti-skid lines are carved on the surface of the rotating block 83, the right side of the tube head 7 is rotatably connected with a baffle 85 through a turntable 84, the bottom of the baffle 85 is in contact with the top of the threaded rod 82, a connecting block 86 is fixedly connected to the right side of the tube head 7 and located above the turntable, one end of the connecting block 86 is fixedly connected with an arc plate 87, the arc plate 87 has certain elasticity and can fix the baffle 85, a rubber plate 88 is fixedly connected to the inner surface of the arc plate 87, the rubber plate 88 can increase friction between the baffle 85 and the arc plate 87, and the size of the arc plate 87 is matched with the size of the baffle 85; the top of the separation tube 4 is connected with a slide bar 9 in a sliding manner, the bottom end of the slide bar 9 penetrates through the separation tube 4 and extends into the separation tube 4, the bottom end of the slide bar 9 is fixedly connected with a piston 10, the piston 10 can generate negative pressure to absorb cell sap, the outer surface of the piston 10 is connected with the inner surface of the separation tube 4 in a sliding manner, a filter screen 12 is fixedly connected between the left side and the right side of the inner wall of the separation tube 4 and below the piston 10, miniature hydraulic rods 13 are fixedly connected with the left side and the right side of the separation tube 4 and below the connecting rod 5, the miniature hydraulic rods 13 are controlled by an external switch and are electrically connected with an external power supply, opposite ends of the two miniature hydraulic rods 13 penetrate through the separation tube 4 and extend into the separation tube 4, opposite ends of the two miniature hydraulic rods 13 are fixedly connected with cleaning plates 14, and the tops of the two cleaning plates 14 are connected with the bottom of the filter screen 12 in a sliding manner, slide bar 9's top fixedly connected with handle 11, handle 11's surface is carved with anti-skidding line, and two cleaning plate 14's opposite side all is provided with clearance sawtooth 15, and clearance sawtooth 15 can clear up and cut up cell and impurity, and the quantity of clearance sawtooth 15 is provided with a plurality ofly, and at cleaning plate 14's surface evenly distributed, and the shape of clearance sawtooth 15 is triangle-shaped.
And those not described in detail in this specification are well within the skill of those in the art.
When the cell culture device works, firstly, the vertical rod 3 is placed at the top of the workbench 1, then the sliding ring 6 slides up and down along the outer surface of the vertical rod 3 by sliding the sliding ring 6, after the tube head 7 slides into the inside of the kit 2 to be contacted with cell sap, the handle 11 is pulled to drive the sliding rod 9 to slide upwards along the inner surface of the separation tube 4, meanwhile, the sliding rod 9 drives the piston 10 to slide upwards along the inner wall of the separation tube 4, meanwhile, the cell sap is sucked into the separation tube 4 through the tube head 7, further, unnecessary cells and impurities are filtered through the filter screen 12, after the suction is finished, the baffle 85 can be taken down from the surface of the arc plate 87, then the baffle is rotated to be contacted with the bottom of the tube head 7 through the turntable 84, the rotating block 83 is further rotated to drive the rotating block 83 to drive the threaded rod 82 to rotate, meanwhile, the threaded rod 82 slides rightwards, and finally, the baffle 85 is blocked, prevent baffle 85 activity, when filter screen 12 takes place to block up, start miniature hydraulic stem 13, make miniature hydraulic stem 13 drive clearance board slide along the bottom of filter screen, clearance sawtooth 15 begins to cut the clearance to unnecessary cell and impurity simultaneously, open baffle 85 once more, promote 11 accessible rivers of handle and wash away impurity, the cell that draws is stayed to the filter screen upper strata, through rotating baffle 85, make baffle 85 and the bottom contact of tube head 7, rethread rotates turning block 83, make turning block 83 drive threaded rod 82 and rotate, make threaded rod 82 slide right simultaneously, and finally block baffle 85, prevent baffle 85 activity, through setting up anti-pollution mechanism 8, tube head 7 has been avoided after absorbing the cell, by external bacterial contamination, the degree of accuracy of experimental result has been improved, it slides to drive clearance board 14 through miniature hydraulic stem 13, clearance board 14 drives clearance sawtooth 15 simultaneously and cuts clearance to adnexed unnecessary cell and impurity on filter screen 12 and clear up The reason has avoided the jam of filter screen 12, and the practicality is stronger, through the setting of slip ring 6, can make slip ring 6 slide along montant 3's surface, and connecting rod 5 drives separator tube 4 simultaneously and slides, and montant 3 can set up separator tube 4 simultaneously, has further avoided separator tube 4 to be polluted, also need not the handheld separator tube 4 operation of staff moreover, and is more convenient.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (3)

1. The utility model provides a cell separator based on extract anti-oxidant active ingredient, includes workstation (1), the top of workstation (1) is provided with kit (2), its characterized in that: the anti-pollution device is characterized in that vertical rods (3) are arranged on the left side and the right side of the top of the workbench (1), the bottoms of the two vertical rods (3) are in contact with the top of the workbench (1), a separation tube (4) is arranged on the top of the kit (2), connecting rods (5) are fixedly connected to the left side and the right side of the separation tube (4), one ends, far away from the two connecting rods (5), of the two connecting rods are fixedly connected with sliding rings (6), the outer surfaces of the two vertical rods (3) are slidably connected with the inner surface of the sliding rings (6), a tube head (7) is fixedly connected to the bottom of the separation tube (4), and an anti-pollution mechanism (8) is arranged on the surface of the tube head (7);
including riser (81) in anti-pollution mechanism (8), the top of riser (81) and the left side fixed connection of tube head (7), the left side threaded connection of riser (81) has threaded rod (82), the right-hand member of threaded rod (82) runs through riser (81) and extends to the right side of riser (81), the left end fixedly connected with turning block (83) of threaded rod (82), the surface sculpture of turning block (83) has anti-skidding line, the right side of tube head (7) is rotated through carousel (84) and is connected with baffle (85), the bottom of baffle (85) and the top contact of threaded rod (82), the right side of tube head (7) just is located the top fixedly connected with connecting block (86) of carousel, the one end fixedly connected with arc board (87) of connecting block (86), the internal surface fixedly connected with rubber slab (88) of arc board (87), the size of the arc plate (87) is matched with that of the baffle plate (85);
the top of the separation tube (4) is connected with a slide bar (9) in a sliding way, the bottom end of the slide bar (9) penetrates through the separation tube (4) and extends to the inside of the separation tube (4), the bottom end of the sliding rod (9) is fixedly connected with a piston (10), the outer surface of the piston (10) is connected with the inner surface of the separating tube (4) in a sliding way, a filter screen (12) is fixedly connected between the left side and the right side of the inner wall of the separation pipe (4) and below the piston (10), the left and right sides of separator tube (4) just are located the equal fixedly connected with miniature hydraulic stem (13) in below of connecting rod (5), two the looks remote site of miniature hydraulic stem (13) all runs through separator tube (4) and extends to the inside of separator tube (4), two the equal fixedly connected with cleaning plate (14) in looks remote site of miniature hydraulic stem (13), two the top of cleaning plate (14) all with the bottom sliding connection of filter screen (12).
2. A cell separator based on the extraction of antioxidant active principles, as claimed in claim 1, characterized by: the top of slide bar (9) fixedly connected with handle (11), the surface sculpture of handle (11) has anti-skidding line.
3. A cell separator based on the extraction of antioxidant active principles, as claimed in claim 1, characterized by: the cleaning plates (14) are provided with a plurality of cleaning sawteeth (15) on the opposite sides, the cleaning sawteeth (15) are uniformly distributed on the surfaces of the cleaning plates (14), and the cleaning sawteeth (15) are triangular.
CN202011620662.0A 2020-12-30 2020-12-30 Cell separator based on extract anti-oxidant active ingredient Active CN112625863B (en)

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CN112625863B true CN112625863B (en) 2022-06-07

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN87209860U (en) * 1987-07-08 1988-05-25 中国人民解放军总医院 Single cell separator
CN105985903A (en) * 2015-02-12 2016-10-05 谭巍 Cell collecting device and application thereof
CN208327986U (en) * 2018-04-29 2019-01-04 江苏伟康洁婧医疗器械股份有限公司 A kind of medical field cell separator

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101179548B1 (en) * 2012-03-05 2012-09-05 김준우 Dividing device for blood elements or cell fractions

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN87209860U (en) * 1987-07-08 1988-05-25 中国人民解放军总医院 Single cell separator
CN105985903A (en) * 2015-02-12 2016-10-05 谭巍 Cell collecting device and application thereof
CN208327986U (en) * 2018-04-29 2019-01-04 江苏伟康洁婧医疗器械股份有限公司 A kind of medical field cell separator

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