CN206630939U - A kind of collection device of leucocyte or monocyte - Google Patents
A kind of collection device of leucocyte or monocyte Download PDFInfo
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- CN206630939U CN206630939U CN201621403494.9U CN201621403494U CN206630939U CN 206630939 U CN206630939 U CN 206630939U CN 201621403494 U CN201621403494 U CN 201621403494U CN 206630939 U CN206630939 U CN 206630939U
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- leucocyte
- monocyte
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Abstract
The utility model discloses a kind of leucocyte or the collection device of monocyte, the collection device includes leucocyte or monocyte collector, the collector includes the housing with inner chamber, filter membrane in housing cavity, collector is also included on housing and positioned at both sides above and below filter membrane, the feed pathway and liquid outlet channel of blood supply liquid disengaging, along direction of flow of blood, filter membrane includes the first film layer being cascading, second film layer, 3rd film layer and the 4th film layer, the first film layer is made up of polyvinylidene fluoride material, second film layer is made up of polyurethane material, 3rd film layer is made up of the glass fiber material doped with carbon fiber, 4th film layer is made up of nylon material.Using the collection device so that the high income of leucocyte or monocyte, up to more than 90%, the Content of Hemocytes obtained in the shortest time is also more, and cell survival rate is also greatly improved, up to more than 80%.
Description
Technical field
It the utility model is related to technical field of medical instruments, and in particular to the collection dress of a kind of leucocyte or monocyte
Put.
Background technology
It is well known that leucocyte is once called as white blood cell, also referred to as immunocyte, and it is a kind of cell in blood, being capable of conduct
A part for immune system helps body resistance infectious disease and external thing, is a kind of important haemocyte in blood, shows
It is colourless karyocyte under micro mirror, it is general spherical in shape.Particle can be divided into and without the major class of particle two according to morphological differences.Particle is white
Cell（Granulocyte）In contain specific stain particle, with Rui Shi dyeings it is distinguishable go out three kinds of granular leucocytes, i.e.,：Neutrophilia
Granulocyte, eosinophil and basophilic granulocyte；Agranular leukocyte includes monocyte and lymphocyte.It is all kinds of white thin
The defence protective effect of born of the same parents is different.And it is current, in order to ensure blood used in clinic safety, transfusion reaction is avoided, generally, people will
Leucocyte in blood is transfused blood again after being filtered out, and the leucocyte after filtering is given up by people mostly, and clinic is carrying
When taking the basal cells of the immunity cell of disease such as treatment malignant tumour, blood disease, basal cell's amount that conventional method obtains is few, living
Property it is low, utilization can not be preserved or feed back autologous by extracting the blood after basal cell, cause great waste.Therefore, how to add
By force the collection to leucocyte or monocyte, using being those skilled in the art's urgent need to solve the problem.
The content of the invention
The purpose of this utility model is overcome the deficiencies in the prior art, there is provided the collection dress of a kind of leucocyte or monocyte
Put, its is simple in construction, can collect leucocyte or monocyte online, and collection efficiency is high, up to 90%；Survival rate is high, reachable
80%, the blood after collection can preserve utilization or feed back back autologous.
To reach above-mentioned purpose, the technical solution adopted in the utility model is：A kind of collection of leucocyte or monocyte
Device, for collecting leucocyte or monocyte in blood, the collection device includes leucocyte or monocyte collector,
The collector includes housing, the filter membrane in the housing cavity with inner chamber, and the collector also includes being located at institute
State on housing and positioned at the filter membrane up and down both sides, the disengaging of blood supply liquid feed pathway and liquid outlet channel, along direction of flow of blood,
The filter membrane includes the first film layer, the second film layer, the 3rd film layer and the 4th film layer being cascading, described
The first film layer is made up of polyvinylidene fluoride material, and the second described film layer is made up of polyurethane material, and described
Three film layers are made up of the glass fiber material doped with carbon fiber, and the 4th described film layer is made up of nylon material.
Preferably, the housing is made of thermoplastic resin.
Preferably, the housing is in oblateness.
Preferably, in the collection device, the blood taking needle, the anticoagulant blood-collecting that include setting gradually according to direction of flow of blood
Bag, the dropping funnel with filter screen, the collector, leucocyte-removing or monocyte blood collection bag, the collection device also include setting
In the leucocyte being arranged in parallel with the leucocyte-removing or monocyte blood collection bag in the collector blood output end
Counter-flushing liquid bag, the leucocyte or monokaryon that are arranged in parallel with the anticoagulant blood-collecting bag on the collector blood input
Cell collecting bag.
It is further preferred that the collection device also includes the blast pipe being arranged in parallel with the collector.
It is further preferred that the collection device also include be connected on leucocyte or the monocyte collecting bag from
Heart pipe, for carrying out centrifugal purification processing to the leucocyte in the leucocyte or monocyte collecting bag or monocyte.
It is further preferred that when the blood that the blood taking needle is gathered comes from allosome, also include in the collection device
The haemocyte nutrient solution bag being connected in leucocyte-removing or the monocyte blood collection bag and empty plasma bags, are used respectively
In haemocyte and the Collection and conservation of blood plasma.
It is further preferred that when the blood that the blood taking needle is gathered comes from autologous, also include in the collection device
The blood transfusion needle being connected in leucocyte-removing or the monocyte blood collection bag, collect and terminate for leucocyte or monocyte
Blood afterwards returns defeated time from vivo.
Due to the utilization of above-mentioned technical proposal, the utility model has following advantages compared with prior art：The present invention's
Collector, its filter membrane used are that film layer is laminated made of four layers of different materials, to leucocyte or monocyte
Absorption affinity is high, online can effectively collect the leucocyte in blood or monocyte, then to leucocyte or monocyte
Purified and induced by adding cell factor, the immunocompetent cell such as manufactured CIK cell can treat pernicious swollen
Many diseases such as knurl, and the leucocyte or the blood of monocyte that gather can be returned defeated time autologous or preservation profit in time
With effectively prevent the waste of blood.
Brief description of the drawings
Accompanying drawing 1 be leucocyte described in the utility model or monocyte collection device in filter membrane sectional view；
Accompanying drawing 2 is the structural representation of the collection device of the leucocyte or monocyte described in embodiment 1；
Accompanying drawing 3 is the structural representation of the collection device of the leucocyte or monocyte described in embodiment 2；
Wherein：1st, blood taking needle；2nd, anticoagulant blood-collecting bag；3rd, dropping funnel；4th, collector；5th, leucocyte-removing or monocyte blood
Collecting bag；51st, haemocyte nutrient solution bag；52nd, empty plasma bags；6th, leucocyte counter-flushing liquid bag；7th, leucocyte or monocyte are received
Collect bag；71st, leucocyte or monocyte collecting pipe；8th, sampler bag；9th, blood transfusion needle；11st, medical catheter；12nd, blast pipe；21、22、
23rd, 24,25,26,27,28, flow-stopping clip, 41, the first film layer；42nd, the second film layer；43rd, the 3rd film layer；44th, the 4th is thin
Film layer.
Embodiment
Below in conjunction with the accompanying drawings and specific embodiment is further elaborated to the technical solution of the utility model.
Embodiment 1
A kind of collection device of leucocyte or monocyte, for collecting leucocyte or monocyte in blood, including
The collector 4 of leucocyte or monocyte, shown in Figure 1, the collector 4 includes the housing with inner chamber, is arranged on housing
Filter membrane in inner chamber, the collector 4 are also included on housing and positioned at the filter membrane feed pathway that both sides, blood supply liquid pass in and out up and down
With liquid outlet channel.It is shown in Figure 1, along direction of flow of blood, the first film layer 41 that the filter membrane includes being cascading, the
Two film layers 41, the 3rd film layer 43 and the 4th film layer 44, the first film layer 41 are made up of polyvinylidene fluoride material, and second
Film layer 41 is made up of polyurethane material, and the 3rd film layer 43 is made up of the glass fiber material doped with carbon fiber, the
Four film layers 44 are made up of nylon material.Using the collector 4 of said structure, while leucocyte or monocyte are collected
Inactivation of virus can also be carried out to blood.
In this example, the housing is made of thermoplastic resin, so as to improve the overall pliability of collector 4 and stretch
Intensity.Housing in this example is in oblateness.
The collection device of leucocyte or monocyte in this example, it is shown in Figure 2, flowed to according to blood flow, the receipts
Acquisition means include blood taking needle 1, anticoagulant blood-collecting bag 2, the dropping funnel 3 with filter screen, collector 4, leucocyte-removing or the monokaryon set gradually
Cellular blood collecting bag 5, the collection device also include in the blood output end of collector 4 with leucocyte-removing or monocyte
Leucocyte counter-flushing liquid bag 6 that blood collection bag 5 is arranged in parallel, on the blood input of collector 4 with anticoagulant blood-collecting bag 2
The leucocyte or monocyte collecting bag 7 being arranged in parallel, the collection device also include being connected to blood taking needle 1 and anticoagulant blood-collecting bag 2
Between the sampler bag 8 being in parallel with anticoagulant blood-collecting bag 2.
In this example, the blood comes from allosome, and the collection device also includes being connected to leucocyte-removing or monocyte blood
Haemocyte nutrient solution bag 51 and empty plasma bags 52 on liquid collecting bag 5, it is respectively used to the Collection and conservation of haemocyte and blood plasma.Entirely
It is connected between each part in collection device by medical catheter 11, and flow-stopping clip is carried in each blood bag, collection dress
Put the blast pipe 12 for also including being connected in parallel on collector 4.
Operation principle is as follows：
1）Open flow-stopping clip 26, remaining fluid stopping clip closure, by blood taking needle 1 by blood collection to anticoagulant blood-collecting bag 2, portion
Divide and collect in sampler bag 8, then close flow-stopping clip 26, sampler bag 8 is subjected to heat seal detachment, kept sample；
2）After blood sampling terminates, anticoagulant blood-collecting bag 2 is placed in eminence, leucocyte-removing or monocyte blood collection bag 5 are placed in
Lower, while flow-stopping clip 21,23 is opened, remaining fluid stopping clip closure, if there is bubble in medical catheter 11, flow-stopping clip 22 is opened, and
By blast pipe 12 by the removal of bubbles in collection device, flow-stopping clip 22 is then closed, the blood in anticoagulant blood-collecting bag 2 is in gravity
In the presence of, flowed into successively after dropping funnel 3, collector 4 filter in leucocyte-removing or monocyte blood collection bag 5；
3）After blood filtration in anticoagulant blood-collecting bag 2 terminates, close flow-stopping clip 21,23, and by anticoagulant blood-collecting bag 2 with go it is white
Cell or monocyte blood collection bag 5 carry out heat seal detachment；
4）Centrifugal treating is carried out to leucocyte-removing or monocyte blood collection bag 5, then by haemocyte nutrient solution bag 51
And be connected after the empty parallel connection of plasma bags 52 with leucocyte-removing or monocyte blood collection bag 5, leucocyte-removing or monocyte blood
Blood in collecting bag 5 is divided into two layers after centrifugation, and upper strata is plasma layer, and lower floor is the blood for eliminating leucocyte or monocyte
Cellular layer, flow-stopping clip 27 is opened, the plasma layer on upper strata is squeezed into sky plasma bags 52；Then by haemocyte nutrient solution bag 51
Nutrient solution squeeze into leucocyte-removing or monocyte blood collection bag 5, cell culture is carried out to the haemocyte of lower floor；
5）Flow-stopping clip 24,25 is opened, the flushing liquor in leucocyte counter-flushing liquid bag 6 is extruded, by the leucocyte in collector 4
It is rinsed to leucocyte or monocyte collecting bag 7；
6）After flushing terminates, flow-stopping clip 24,25 is closed, by leucocyte or the heat seal detachment of monocyte collecting bag 7, and will be white
Cell or monocyte collecting bag 7 carry out centrifugal treating, and the leucocyte after separation or monocyte are squeezed into monokaryon after leucocyte
Cell collecting pipe 71, subsequently leucocyte or monocyte are purified again, prepare immunocompetent cell.
The collection device of leucocyte or monocyte in this example, whole collection process are in a closed system, no
Any pollution can be caused to blood, while compared with prior art, leucocyte or monocyte can be collected online, most in short-term
Between the haemocyte that is obtained or monocyte content it is more, for collection rate up to 90%, cell survival rate has also obtained very big carry
Height, up to more than 80%.
Embodiment 2
The difference of this example and embodiment 1 is, the blood gathered in this example is from autologous, it is therefore not necessary to will go again white
Cell or monocyte blood collection bag 5 carry out centrifugal treating, shown in Figure 3, without empty plasma bags 52 and haemocyte culture
51 two parts of liquid bag, directly blood transfusion tube can be connected on leucocyte-removing or monocyte blood collection bag 5, pass through blood transfusion needle
9 return the blood syringe for eliminating leucocyte or monocyte from vivo, while the leucocyte of acquisition or monocyte are carried out
Purification process, then cultivate clinical required immunity cell.
For above-described embodiment only to illustrate technical concepts and features of the present utility model, its object is to allow be familiar with technique
Personage can understand content of the present utility model and implement according to this, the scope of protection of the utility model can not be limited with this.
All equivalent change or modifications made according to the utility model Spirit Essence, should all cover the scope of protection of the utility model it
It is interior.
Claims (8)
- A kind of 1. collection device of leucocyte or monocyte, for collecting leucocyte or monocyte in blood, its feature It is, the collection device includes leucocyte or monocyte collector, and the collector includes the housing with inner chamber, is located at Filter membrane in the housing cavity, the collector are also included on the housing and positioned at filter membrane both sides, confession up and down The feed pathway and liquid outlet channel of blood disengaging, along direction of flow of blood, the filter membrane includes being cascading first thin Film layer, the second film layer, the 3rd film layer and the 4th film layer, described the first film layer is by polyvinylidene fluoride material system Into the second described film layer is made up of polyurethane material, and the 3rd described film layer is by the glass doped with carbon fiber Fibrous material is made, and the 4th described film layer is made up of nylon material.
- 2. the collection device of leucocyte according to claim 1 or monocyte, it is characterised in that the housing is by heat Made of plastic resin.
- 3. the collection device of leucocyte according to claim 1 or 2 or monocyte, it is characterised in that the housing is in It is oblate.
- 4. the collection device of leucocyte according to claim 1 or monocyte, it is characterised in that the collection device In, the blood taking needle that includes setting gradually according to direction of flow of blood, anticoagulant blood-collecting bag, the dropping funnel with filter screen, the collector, go Leucocyte or monocyte blood collection bag, the collection device also include in the collector blood output end with institute State leucocyte-removing or leucocyte counter-flushing liquid bag that monocyte blood collection bag is arranged in parallel, defeated located at the collector blood Enter the leucocyte or monocyte collecting bag that are arranged in parallel with the anticoagulant blood-collecting bag on end.
- 5. the collection device of leucocyte according to claim 4 or monocyte, it is characterised in that the collection device is also Including the blast pipe being arranged in parallel with the collector.
- 6. the collection device of leucocyte according to claim 4 or monocyte, it is characterised in that the collection device is also Including the centrifuge tube being connected on leucocyte or the monocyte collecting bag, for the leucocyte or monocyte collection Leucocyte or monocyte in bag carry out centrifugal purification processing.
- 7. the collection device of leucocyte according to claim 4 or monocyte, it is characterised in that when the blood taking needle institute Also include being connected to leucocyte-removing or the monocyte blood when blood of collection comes from allosome, in the collection device Haemocyte nutrient solution bag and empty plasma bags on collecting bag, it is respectively used to the Collection and conservation of haemocyte and blood plasma.
- 8. the collection device of leucocyte according to claim 4 or monocyte, it is characterised in that when the blood taking needle institute Also include being connected to leucocyte-removing or the monocyte blood collection when blood of collection comes from autologous, in the collection device Blood transfusion needle on bag, the blood collected for leucocyte or monocyte after terminating return defeated time from vivo.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621403494.9U CN206630939U (en) | 2016-12-20 | 2016-12-20 | A kind of collection device of leucocyte or monocyte |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621403494.9U CN206630939U (en) | 2016-12-20 | 2016-12-20 | A kind of collection device of leucocyte or monocyte |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206630939U true CN206630939U (en) | 2017-11-14 |
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ID=60260757
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621403494.9U Active CN206630939U (en) | 2016-12-20 | 2016-12-20 | A kind of collection device of leucocyte or monocyte |
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| CN (1) | CN206630939U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111939599A (en) * | 2020-07-14 | 2020-11-17 | 山东中保康医疗器具有限公司 | Method and device for preparing platelet-rich plasma |
| CN114410441A (en) * | 2022-02-08 | 2022-04-29 | 浙江嘉孚生物科技有限公司 | Blood collection card and application thereof |
-
2016
- 2016-12-20 CN CN201621403494.9U patent/CN206630939U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111939599A (en) * | 2020-07-14 | 2020-11-17 | 山东中保康医疗器具有限公司 | Method and device for preparing platelet-rich plasma |
| CN111939599B (en) * | 2020-07-14 | 2024-01-12 | 山东中保康医疗器具有限公司 | Method and device for preparing platelet-rich plasma |
| CN114410441A (en) * | 2022-02-08 | 2022-04-29 | 浙江嘉孚生物科技有限公司 | Blood collection card and application thereof |
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