Background
Platelet Rich Plasma (PRP) preparation sets on the market at present are of various types, but the platelet in the plasma is separated by centrifugal tube centrifugation basically to prepare the platelet rich plasma.
The centrifugal tube type Platelet Rich Plasma (PRP) preparation suit is easy to realize in product structure, but is tedious in the actual operation process, the influence of human factors on the prepared platelet rich plasma is large, the repeatability is poor, and a better preparation effect can be achieved by a large amount of practice of professionals.
In addition, Platelet Rich Plasma (PRP) is often prepared in an open procedure and is susceptible to contamination by bacteria.
SUMMERY OF THE UTILITY MODEL
The to-be-solved technical problem of the utility model is: overcomes the defects in the prior art, and provides a platelet-rich plasma preparation device which separates platelet-rich plasma by adopting a membrane filtration method and has simple and convenient operation process.
The platelet-rich plasma preparation device of the utility model comprises a shell, a first filter membrane and a second filter membrane which are fixed in the shell, wherein the inner cavity of the shell is divided into three layers by the two filter membranes; one end of the shell is provided with a whole blood inlet and a blood outlet, and the other end is provided with a plasma outlet; the aperture of the first filter membrane is 3-5 mu m, and the first filter membrane is close to one side of the whole blood inlet; the aperture of the second filter membrane is smaller than 2 μm and is close to one side of the plasma outlet; a chamber between the two filter membranes is a platelet collecting chamber; the whole blood inlet is connected with a whole blood input pipe, and a peristaltic pump is arranged on the whole blood input pipe; the blood outlet is connected with a blood output tube, and a first liquid stopping clamp is arranged on the blood output tube; the plasma outlet is connected with a plasma output tube, and a second liquid stopping clamp is arranged on the plasma output tube.
Because the platelet diameter is 2 ~ 3 mu m in the human whole blood, and the average diameter of erythrocyte is about 9 mu m, and the leucocyte diameter is 7 ~ 20 mu m, consequently, the utility model discloses a membrane filtration method separates the rich platelet plasma in the whole blood and prepares out. Preparation facilities's theory of operation process as follows:
the utility model discloses a shell inner chamber passes through first filter membrane, three-layer cavity structure is separated into to the second filter membrane, blood gets into the first layer cavity of shell from the whole blood import, red blood cell and leucocyte in the whole blood can effectively be blockked to first filter membrane (the aperture of membrane material is 3-5 μm) between first layer cavity and the second floor cavity, and blood components such as platelet can effectively pass first filter membrane and get into in second floor cavity, because the membrane material aperture of the second filter membrane between second floor cavity and the third layer cavity is less than 2 μm, consequently can effectively block the platelet through the second filter membrane, only plasma protein, moisture etc. can penetrate the second filter membrane, platelet-rich plasma stays in second floor cavity (platelet collection chamber) like this; after the first liquid stopping clamp is opened, the blood after separation treatment in the first layer of cavity is directly discharged from the blood outlet; after the second liquid stopping clamp is opened, the plasma is output through the plasma output tube.
Compare traditional centrifugal tube formula Platelet Rich Plasma (PRP) preparation suit, adopt the preparation facilities preparation platelet rich plasma's operation is more simple swift, realizes easily.
In addition, the plasma output by the plasma output tube is mixed with the platelet rich plasma prepared by the preparation device according to a certain proportion, so that the platelet rich plasma with different concentrations can be prepared. It should be noted that the concentration of the prepared platelet-rich plasma can be calculated as follows: the blood component equipment can be used for counting the platelets in the platelet rich plasma prepared by the preparation device, and then the concentration of the prepared platelet rich plasma can be calculated.
The shape of the preparation device is not limited, and the preparation device can be in various shapes such as flat shape, cylinder shape and the like. The filter membranes (including the first filter membrane and the second filter membrane) can be porous polymer membranes, or can be membranes formed by gas-impermeable materials in a laser perforation or ray perforation mode, and the form is not limited.
Preferably, the first filter membrane and the second filter membrane are fixed in the shell through a first support frame and a second support frame respectively.
Preferably, a support member is fixed on the membrane surface of the first filter membrane close to the second filter membrane or the membrane surface of the second filter membrane close to the first filter membrane, and the first filter membrane and the second filter membrane can be separated by the support member to prevent the first filter membrane and the second filter membrane from being attached to each other. Of course, the distance between the first filter membrane and the second filter membrane can be increased to prevent the first filter membrane and the second filter membrane from being attached.
Preferably, a platelet-rich plasma output tube is arranged on the shell corresponding to the position of the platelet collection cavity; and a third liquid stopping clip is arranged on the platelet-rich plasma output tube. And opening the third liquid stopping clamp, and outputting the platelet-rich plasma collected in the platelet collecting cavity through a platelet-rich plasma output tube so as to obtain the required platelet-rich plasma. In actual operation, the needle of the intravenous infusion set can also be directly penetrated into the platelet collection cavity of the shell, and then platelet-rich plasma is extracted.
Preferably, the plasma output tube is connected with a first circulation bypass, the other end of the first circulation bypass is connected into the whole blood input tube, and a first peristaltic pump is mounted on the first circulation bypass; the first circulation bypass is connected to the section of the plasma output pipe between the plasma outlet and the second liquid stopping clamp. During operation, the second liquid stopping clamp is closed, the first peristaltic pump is started at the same time, plasma flowing out of the plasma outlet can enter the preparation device through the first circulation bypass and the whole blood inlet, and the preparation rate of the platelet-rich plasma is improved through one or more repeated circulation.
The utility model provides a whole blood input tube can be the whole blood conveying line who is connected with the blood storage container, and the installation second peristaltic pump on the whole blood conveying line, because of the whole blood in the blood storage container has added the anticoagulant, so accessible second peristaltic pump directly carries the whole blood in the blood storage container to the preparation facilities in.
The whole blood input tube in the utility model can also be a blood sampling tube, a third peristaltic pump is arranged on the blood sampling tube, an anticoagulant tube is connected on the blood sampling end of the blood sampling tube, and a fourth liquid stopping clamp is arranged on the anticoagulant tube; the anticoagulant line is positioned before the third peristaltic pump. Blood collected from a human body needs to be timely added with anticoagulant to prevent the collected blood from coagulating, and the blood added with the anticoagulant is conveyed to the preparation device through the third peristaltic pump.
Preferably, the blood output tube is connected with a return tube for conveying the blood back to the human body; the blood conveying pipeline is connected to a blood output pipe section between the blood outlet and the first liquid stopping clamp, and a fourth peristaltic pump is installed on the blood conveying pipeline; in order to ensure the osmotic pressure balance between the blood in the human body and the blood in the human body, the blood returning pipeline is connected with a physiological saline pipeline, and a fifth liquid stopping clamp is arranged on the physiological saline pipeline. When the blood output by the blood output tube needs to be conveyed back to the human body, the first liquid stopping clamp needs to be closed, the fourth peristaltic pump is started, meanwhile, the fifth liquid stopping clamp is opened to add the physiological saline into the blood in the return conveying pipeline, and the blood is conveyed back to the human body through the fourth peristaltic pump.
Preferably, the blood output tube is connected with a second circulation bypass, the other end of the second circulation bypass is connected into the whole blood input tube, and a fifth peristaltic pump is mounted on the second circulation bypass; the second circulation bypass is connected to the blood output tube section between the blood outlet and the first liquid stopping clamp. During operation, the first liquid stopping clamp needs to be closed, after the fifth peristaltic pump is started, blood flowing out of the blood outlet enters the preparation device through the second circulation bypass and the whole blood inlet, and the preparation rate of the platelet-rich plasma is improved through one or more repeated circulation.
Compared with the prior art, the utility model beneficial effect who has is:
the utility model discloses a membrane filtration method fixes different blood components at a certain aspect through the filter membrane of two-layer different apertures to reach the effect of separation, thereby prepare out the rich platelet plasma separation in the whole blood, compare traditional centrifugation tubular rich platelet plasma (PRP) preparation suit, adopt the operation of preparation facilities preparation rich platelet plasma is more simple swift, realizes easily. In addition, the plasma output by the plasma output tube is mixed with the platelet rich plasma prepared by the preparation device according to a certain proportion, so that the platelet rich plasma with different concentrations can be prepared.
Detailed Description
Embodiments of the present invention are further described below with reference to the accompanying drawings:
the first embodiment is as follows:
as shown in fig. 1, the platelet rich plasma preparation device of this embodiment comprises a housing 1, and a first filter 16 and a second filter 21 fixed in the housing 1, wherein the two filters separate the inner cavity of the housing 1 into three layers; one end of the shell 1 is provided with a whole blood inlet 2 and a blood outlet 15, and the other end is provided with a plasma outlet 23; the aperture of the first filter membrane 16 is 3-5 μm, and the first filter membrane is close to one side of the whole blood inlet 2; the aperture of the second filter membrane 21 is less than 2 μm, and is close to the plasma outlet 23 side; the cavity between the two filter membranes is a platelet collection cavity 28; the whole blood inlet 2 is connected with a whole blood input pipe, and a peristaltic pump is arranged on the whole blood input pipe; the blood outlet 15 is connected with a blood output tube 9, and a first liquid stopping clamp 10 is arranged on the blood output tube 9; the plasma outlet 23 is connected with a plasma output tube 24, and a second liquid stopping clamp 25 is arranged on the plasma output tube 24.
Because the platelet diameter is 2 ~ 3 mu m in the human whole blood, and the average diameter of erythrocyte is about 9 mu m, and the leucocyte diameter is 7 ~ 20 mu m, consequently, the utility model discloses a membrane filtration method separates the platelet in the whole blood. The working principle and the process of the preparation device are as follows:
the inner cavity of the shell 1 of the embodiment is divided into a three-layer cavity structure by the first filter membrane 16 and the second filter membrane 21, blood enters the first layer cavity of the shell 1 from the whole blood inlet 2, the first filter membrane 16 (the pore diameter of the membrane material is 3-5 μm) between the first layer cavity and the second layer cavity can effectively block red blood cells and white blood cells in the whole blood, blood components such as platelets and the like can effectively penetrate the first filter membrane 16 and enter the second layer cavity, and as the pore diameter of the membrane material of the second filter membrane 21 between the second layer cavity and the third layer cavity is smaller than 2 μm, platelets can be effectively blocked by the second filter membrane 21, only plasma proteins, moisture and the like can penetrate the second filter membrane 21, so that platelet-rich plasma is left in the second layer cavity (namely the platelet collection cavity 28); after the first liquid stopping clamp 10 is opened, the blood after separation treatment in the first layer chamber is directly discharged from the blood outlet 15; after the second stop clamp 25 is opened, the plasma is output through the plasma output tube 24.
Compared with the traditional centrifugal tube type Platelet Rich Plasma (PRP) preparation set, the preparation device of the embodiment is simpler, quicker and easier to operate and realize.
In addition, the plasma output by the plasma output tube 24 is mixed with the platelet rich plasma prepared by the preparation device according to a certain proportion, so that the platelet rich plasma with different concentrations can be prepared. It should be noted that the concentration of the prepared platelet-rich plasma can be calculated as follows: the blood component equipment can be used for counting the platelets in the platelet rich plasma prepared by the preparation device, and then the concentration of the prepared platelet rich plasma can be calculated.
In this embodiment:
the first filter membrane 16 and the second filter membrane 21 are respectively fixed in the shell 1 through a first support frame 17 and a second support frame 20; the support member 22 is welded by ultrasonic on the membrane surface of the first filter membrane 16 close to the second filter membrane or the membrane surface of the second filter membrane 21 close to the first filter membrane, and the support member 22 can separate the first filter membrane 16 from the second filter membrane 21, thereby preventing the first filter membrane 16 from being attached to the second filter membrane 21.
A platelet-rich plasma output tube 19 is arranged on the shell 1 corresponding to the position of the platelet collection cavity 28; the platelet-rich plasma output tube 19 is provided with a third liquid stopping clamp 18. The third liquid stopping clamp 18 is opened, and the platelet-rich plasma collected in the platelet collecting cavity 28 is output through the platelet-rich plasma output tube 19, so that the required platelet-rich plasma is obtained.
The plasma output tube 24 is connected with a first circulation bypass 26, the other end of the first circulation bypass 26 is connected into the whole blood input tube, and a first peristaltic pump 27 is arranged on the first circulation bypass 26; the first bypass 26 is connected to the plasma outlet line section between the plasma outlet 23 and the second liquid stop clamp 25. In operation, the second liquid stopping clamp 25 is closed, and the first peristaltic pump 27 is started, so that the plasma flowing out of the plasma outlet 23 can enter the preparation device through the first circulation bypass 26 and the whole blood inlet 2, and the preparation rate of the platelet-rich plasma is improved through one or more repeated circulation.
The whole blood input tube may be a whole blood feeding tube 30 connected to a blood storage container 31, and the whole blood feeding tube 30 is provided with a second peristaltic pump 29, so that the whole blood in the blood storage container 31 can be directly fed to the preparation device through the second peristaltic pump 29 because the whole blood in the blood storage container 31 is added with anticoagulant. The whole blood input pipe can also be a blood sampling pipeline 3, a third peristaltic pump 6 is arranged on the blood sampling pipeline 3, an anticoagulant pipeline 5 is connected to the blood sampling end of the blood sampling pipeline 3, and a fourth liquid stopping clamp 4 is arranged on the anticoagulant pipeline 5; anticoagulant line 5 is located before third peristaltic pump 6. Blood collected from a human body needs to be timely added with anticoagulant to prevent the collected blood from coagulating, and the blood added with anticoagulant is conveyed into the preparation device through the third peristaltic pump 6.
The blood output tube 9 is connected with a transfusion tube 13 which conveys the blood back to the human body; the transfusion pipeline 13 is connected to a blood output pipeline section between the blood outlet 15 and the first liquid stopping clamp 10, and the transfusion pipeline 13 is provided with a fourth peristaltic pump 14; in order to ensure the osmotic pressure balance between the blood returned to the human body and the blood in the human body in the return pipeline 13, the return pipeline 13 is connected with a physiological saline pipeline 11, and the physiological saline pipeline 11 is provided with a fifth liquid stopping clamp 12. When the blood output by the blood output tube 9 needs to be delivered back to the human body, the first liquid stop clamp 10 needs to be closed, the fourth peristaltic pump 14 needs to be started, meanwhile, the fifth liquid stop clamp 12 needs to be opened to add the physiological saline into the blood in the return tube 13, and the blood is delivered back to the human body through the fourth peristaltic pump 14.
The blood output tube 9 is connected with a second circulation bypass 7, the other end of the second circulation bypass 7 is connected into the whole blood input tube, and a fifth peristaltic pump 8 is arranged on the second circulation bypass 7; the second bypass 7 is connected to the blood outlet line section between the blood outlet 15 and the first liquid stop clamp 10. During operation, the first liquid stopping clamp 10 needs to be closed, the fifth peristaltic pump 8 is started, blood flowing out of the blood outlet 15 enters the preparation device through the second circulation bypass 7 and the whole blood inlet 2, and the preparation rate of the platelet-rich plasma is improved through one or more repeated circulation.
The preparation device of this embodiment is not limited in shape, and may be in various forms such as flat, cylindrical, etc. The filter membranes (including the first filter membrane 16 and the second filter membrane 21) may be porous polymer membranes, or may be membranes formed by laser drilling or radiation drilling of gas-impermeable materials, and the form is not limited.
Example two:
as shown in fig. 2, the platelet rich plasma preparation device of this embodiment has substantially the same structure as that of the first embodiment, except that the whole blood inlet 2 and the blood outlet 15 are located above and the plasma outlet 23 is located below, which is equivalent to reversing the whole structure of the first embodiment from top to bottom. However, the working principle and process of this embodiment are exactly the same as those of the first embodiment, and will not be described here.