CN101787345A - Device for collecting and separating body fluid sample used for cell examination and method therefor - Google Patents
Device for collecting and separating body fluid sample used for cell examination and method therefor Download PDFInfo
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- CN101787345A CN101787345A CN 201010120630 CN201010120630A CN101787345A CN 101787345 A CN101787345 A CN 101787345A CN 201010120630 CN201010120630 CN 201010120630 CN 201010120630 A CN201010120630 A CN 201010120630A CN 101787345 A CN101787345 A CN 101787345A
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Abstract
The invention relates to a device for collecting and separating a body fluid sample used for cell examination and a method therefor. The device comprises a sample collection container and a cell smear device, wherein the sample collection container comprises a container body with an opening at the top part; a lateral liquid discharge hole provided with a liquid discharge hole switch is arranged at the lower part of the side wall of the container body; the bottom of the container body is of the V shape or the turbination; and a T-shaped collection pipe is arranged at the bottom of the V shape or the turbination. The method comprises: adding various body fluid samples into the container body for resting, so that the visible components such as cells and the like in the body fluid samples can be naturally precipitated into the collection pipe, and the liquid supernatant of the body fluid can be discharged by the liquid discharge hole; and then taking down the collection pipe and directly fixing the collection pipe on the cell smear device, and adopting a general centrifugation smear machine to prepare a cell smear directly. The device and the method can successfully collect large amount of the visible components such as cells, sediment and the like in the body fluid sample, can achieve the aim of obviously improving the relevance ratio of positive cells and can be widely used for the cytological examination of various body fluid samples.
Description
Technical field
The invention belongs to cytolgical examination utensil in the medicine equipment of medical field, be used for body fluid sample collection, tripping device and the method for cytoscopy in particularly a kind of available clinical body fluid sample (hydrothorax, ascites, urine, cerebrospinal fluid, drainage liquid) and cell culture fluid, the inoculum.
Background technology
Carrying out cytolgical examination for body fluid samples such as hydrothorax, ascites, urine, cerebrospinal fluid, various body cavity drainage liquid is daily curative activity, particularly the important component part of cell pathology worker routine work.Can effectively collect, separate the formed elements in the above-mentioned body fluid sample, can particularly various cast-off cells be directly connected to quality, cell pathology diagnosis and the positive rate of cell smear, also determine the patient be made a definite diagnosis timely and effectively treatment.Can the formed elements that therefore, effectively collect, separate in the body fluid samples such as hydrothorax, ascites, urine, cerebrospinal fluid, various body cavity drainage liquid be the important prerequisite that guarantees this work.
But, in daily medical practice, general various body cavity drainage liquid is usually more, hydrothorax particularly, ascites, body fluid samples such as urine are bulky especially, often hundreds of at least milliliters, thousands of at most milliliters, therefore, if just extracting a small amount of sample carries out cytolgical examination and has the huge risk of failing to pinpoint a disease in diagnosis undoubtedly, but, owing to lack suitable utensil at present, therefore, will be to all body fluid samples (as hundreds of milliliters hydrothorax, ascites, or thousands of milliliters twenty-four-hour urine fluid samples) cast-off cells collects in, separation be one loaded down with trivial details and be difficult to obtain the treatment process of promising result, generally at present adopt following method: (1) is used and is outwelled supernatant after bigger container (as infusion bottle) leaves standstill, and the liquid smear of drawing container bottom again carries out cytolgical examination; (2) adopt the large vol centrifuge tube to above-mentioned body fluid sample gradation, carry out centrifugation in batches, be collected in one, smear carries out cytolgical examination then; Obviously, aforesaid method is very loaded down with trivial details, but also might also have the risk of polluting simultaneously because the part cell adheres to still to cause on the tube wall and fails to pinpoint a disease in diagnosis.
As seen, adopt existing method that body fluid sample is carried out the shortcoming that loaded down with trivial details, the easy pollution of cytolgical examination work, efficient are low, easily fail to pinpoint a disease in diagnosis.
Equally, also there are the problems referred to above for cell, bacterium in the cell cultures in life science field, the microbial culture in separating.
Therefore, adapt to the clinical and cytopathology need of work of actual medical, research and development can improve body fluid sample collection, the tripping device using method of body fluid sample positive rate, have important economic benefit and social benefit.
Summary of the invention
The objective of the invention is to defective or deficiency at above-mentioned prior art existence, provide a kind of body fluid sample that is used for cytoscopy to collect, tripping device and method, be used for collecting, formed elements pair cell pathological examination such as cell and research provide basic basis in the various body fluid thereby separate, and provide a kind of use said apparatus to collect fast, the method of separating formed elements such as cell in the various body fluid samples, this method can overcome the defective of existing method, particularly overcomes under the situation that the body fluid sample amount reaches hundreds if not thousands of milliliters to collect, the shortcoming that prior art exists during cast-off cells in the separating sample.
For achieving the above object, the inventive system comprises sample collection container and cell smear device, said sample collection container comprises that the top is provided with the container body of opening, lower sidewall at container body is provided with a horizontal outage that has the outage switch, the bottom of container body is " V " font or turbination, is provided with "T"-shaped collection tube at " V " font or turbination bottom.
Container body of the present invention and "T"-shaped collection tube adopt the connection type bag-like container of integrated preparation, and filling orifice is installed on opening, and container body top is provided with and is used to the container suspender belt hole that keeps container stable.
Container body of the present invention is bottle or tank body, "T"-shaped collection tube adopts dismountable mode to be arranged on the bottom of container body, the cell smear device that matches with "T"-shaped collection tube comprises a size and common slide glass same fixed groove, be provided with the aperture that is used to discharge unnecessary liquid and unloading slide glass in the pickup groove bottom, pickup groove upper limb both sides are respectively arranged with two symmetric retaining clips that are used to the retaining clip open holes of U-shaped retaining clip is installed and be locked retaining clip and link up with, be disposed with slide glass and pad above the pickup groove, the pad center offer one with the identical through hole of "T"-shaped collection tube openings of sizes, the cell smear device is installed on the "T"-shaped collection tube by the U-shaped retaining clip; The opening at container body top is provided with the lid that has filling orifice; "T"-shaped collection tube peristome be provided with one vertical with "T"-shaped collection tube tube wall and with "T"-shaped collection tube mouth of pipe place and same plane, size is identical with slide glass or be slightly less than the collection tube retaining plate of slide glass; The bottom of container body is provided for keeping stable pan straddle of container or container top that container suspender belt hole is set.
The body fluid sample collection, tripping device and the method that are used for cytoscopy, comprise the following steps: 1) select the container body of suitable size according to the amount of pending body fluid, make the container body horizontal positioned by pan straddle or container suspender belt hole, directly hydrothorax, ascites, urine, cerebrospinal fluid, draining fluids and cell culture fluid and inoculum are joined in the container body from the upper end, perhaps be connected, directly collect body fluid with drainage tube; 2) body fluid leaves standstill more than the 20min after joining container body, makes that the various formed elementss such as cell in the above-mentioned body fluid sample deposit in the "T"-shaped collection tube of container body bottom " V " font or turbination bottom naturally; 3) treat that various formed elements natural subsidence such as cell in the sample are slipped in the "T"-shaped collection tube after, open the outage switch of container body sidewall the body fluid supernatant discharged by outage; 4), surpass humor collecting container primary treatment ability, then carry out step 1)～3 repeatedly if the body fluid sample amount is big) method collects the formed elementss such as cell in the sample in the "T"-shaped collection tube smoothly; 5) after all body fluid sample was finished dealing with, the tilt container body was poured out remaining body fluid supernatant; If container body and "T"-shaped collection tube adopt the connection type bag-like container of integrated preparation, then directly cut "T"-shaped collection tube with scissors, re-use common cell smear and prepare apparatus and prepare cell smear; If container body is bottle or tank body, then take off "T"-shaped collection tube, add Upper gasket, slide glass and pickup groove successively at its top, utilize the U-shaped retaining clip pad, slide glass and pickup groove to be fixed on the retaining plate at "T"-shaped collection tube mouth of pipe position, put it in the rotor or rotary head hanging basket of cell centrifugation smearing machine direct centrifugal preparation cell smear then.
The present invention uses the bottom to be the collection container body fluid sample of " V " font or inversed-cone shape, utilize action of gravity, make various formed elements natural subsidence such as cell in the body fluid sample, be slipped to the collection tube that is positioned at the minimum position of container, discharge the body fluid sample supernatant liquor by the outage that is positioned at the bottle side wall bottom, reach the purpose of formed elementss such as cast-off cells in smooth collection, the chorista fluid samples.Simultaneously,, can directly the formed elementss such as cast-off cells in the collection tube evenly be coated on the slide glass preparation cell smear by using the cell smear device that is complementary with existing centrifugal smearing machine rotor.
If the collection tube of collection container bottom and bottle (or tank body or bag) then directly adopt scissors to cut collection tube for adopting the connection type structure of integrated design preparation, use centrifugal smearing machine to carry out the further separation and the film-making of formed elements in the body fluid sample.
Certainly, these apparatus and method also can be used for separating and the smear preparation of cell (bacterium) in cell cultures and the inoculum.
Description of drawings
Fig. 1 is the structural representation of the sample collection container of the embodiment of the invention 1 integrated design; Fig. 2 is the structural representation of the sample collection container of the embodiment of the invention 2; Fig. 3 is the structural representation of the embodiment of the invention 2 cell smear devices; Fig. 4 is the scheme of installation of the embodiment of the invention 2 "T"-shaped collection tubes and cell smear device; Fig. 5 is the structural representation after the embodiment of the invention 2 "T"-shaped collection tubes and cell smear device are installed; Wherein: 1 container body, 1,2 openings, 3 lids, 4 filling orifices, 5 outages, 6 outage switches, 7 "T"-shaped collection tubes, 8 collection tube retaining plates, 9 pan straddles, 10 container suspender belt holes, 11 pickup grooves, 12 apertures, 13,14 retaining clips hook, 15 retaining clips, 16 pads, 17 slide glasss.
Embodiment
The present invention is described in further detail according to specific embodiment that technical scheme of the present invention is finished below in conjunction with accompanying drawing and contriver.
Referring to Fig. 1～5, Fig. 1～5 also are specific embodiments of the invention, but the invention is not restricted to this embodiment.
Embodiment 1: referring to Fig. 1, the present invention includes sample collection container and cell smear device, said sample collection container comprises that the top is provided with the container body 1 of opening 2, lower sidewall at container body 1 is provided with a horizontal outage 5 that has outage switch 6, the bottom of container body 1 is " V " font or turbination, be provided with "T"-shaped collection tube 7 at " V " font or turbination bottom, wherein container body 1 and "T"-shaped collection tube 7 adopt the connection type bag-like container of integrated preparation, and filling orifice 4 is installed on opening 2, and container body 1 top is provided with and is used to the container suspender belt hole 10 that keeps container stable.
The body fluid sample of its cytoscopy is collected, separation method is: the container body 1 of 1) selecting suitable size according to the amount of pending body fluid, make container body 1 horizontal positioned by container suspender belt hole 10, directly hydrothorax, ascites, urine, cerebrospinal fluid, draining fluids and cell culture fluid and the inoculum filling orifice 4 from container body 1 upper end is joined in the container body 1, perhaps be connected, directly collect body fluid with drainage tube; 2) body fluid leaves standstill more than the 20min after joining container body 1, makes that the various formed elementss such as cell in the above-mentioned body fluid sample deposit in the "T"-shaped collection tube 7 of container body 1 bottom " V " font or turbination bottom naturally; 3) treat that various formed elements natural subsidence such as cell in the sample are slipped in the "T"-shaped collection tube 7 after, open the outage switch 6 of container body 1 sidewall the body fluid supernatant discharged by outage 5; 4), surpass humor collecting container primary treatment ability, then carry out step 1)～3 repeatedly if the body fluid sample amount is big) method collects the formed elementss such as cell in the sample in the "T"-shaped collection tube 7 smoothly; 5) after all body fluid samples were finished dealing with, tilt container body 1 was poured out remaining body fluid supernatant, directly cuts "T"-shaped collection tube 7, re-used common cell smear and prepared apparatus and prepare cell smear; Above-mentioned "T"-shaped collection tube 7 also can be used as centrifuge tube, uses generic centrifuge to carry out the further separation of formed elements in the body fluid sample.If formed elements quantity such as cast-off cells is more in the "T"-shaped collection tube, can preserve standbyly, also can draw the part cellular constituent as different purposes with pipettor according to the real work needs.
Embodiment 2: referring to Fig. 2～5, the present invention includes sample collection container and cell smear device, said sample collection container comprises that the top is provided with the bottle of opening 2 or the container body 1 that tank body is made, the opening 2 at container body 1 top is provided with the lid 3 that has filling orifice 4, the bottom of container body 1 is provided for keeping stable pan straddle of container 9 or container top that container suspender belt hole 10 is set, the lower sidewall of container body 1 is provided with a horizontal outage 5 that has outage switch 6, the bottom of container body 1 is " V " font or turbination, be provided with dismountable "T"-shaped collection tube 7 at " V " font or turbination bottom, said cell smear device comprises a size and common slide glass 17 same fixed grooves 11, be provided with the aperture 12 that is used to discharge unnecessary liquid and unloading slide glass 17 in pickup groove 11 bottoms, pickup groove 11 upper limb both sides are respectively arranged with two symmetric retaining clips that are used to the retaining clip open holes 13 of U-shaped retaining clip 15 is installed and be locked retaining clip 15 and link up with 14, be disposed with slide glass 17 and pad 16 above the pickup groove 11, pad 16 centers offer one with the identical through hole of "T"-shaped collection tube 7 openings of sizes, said "T"-shaped collection tube peristome be provided with one vertical with "T"-shaped collection tube 7 tube walls and with "T"-shaped collection tube 7 mouth of pipe places and same plane, size is identical with slide glass 17 or be slightly less than the collection tube retaining plate 8 of slide glass 17, and the cell smear device is installed on the collection tube retaining plate 8 of "T"-shaped collection tube 7 outers by U-shaped retaining clip 15.
The body fluid sample of its cytoscopy is collected, separation method, comprise the following steps: 1) select the container body 1 of suitable size according to the amount of pending body fluid, make container body 1 horizontal positioned by pan straddle 9 or container suspender belt hole 10, directly hydrothorax, ascites, urine, cerebrospinal fluid, draining fluids and cell culture fluid and inoculum are joined in the container body 1 from the upper end, perhaps be connected, directly collect body fluid with drainage tube; 2) body fluid leaves standstill more than the 20min after joining container body 1, makes that the various formed elementss such as cell in the above-mentioned body fluid sample deposit in the "T"-shaped collection tube 7 of container body 1 bottom " V " font or turbination bottom naturally; 3) treat that various formed elements natural subsidence such as cell in the sample are slipped in the "T"-shaped collection tube 7 after, open the outage switch 6 of container body 1 sidewall the body fluid supernatant discharged by outage 5; 4), surpass humor collecting container primary treatment ability, then carry out step 1)～3 repeatedly if the body fluid sample amount is big) method collects the formed elementss such as cell in the sample in the "T"-shaped collection tube 7 smoothly; 5) after all body fluid sample is finished dealing with, tilt container body 1 is poured out remaining body fluid supernatant, container body 1 is bottle or tank body, then take off "T"-shaped collection tube 7, add Upper gasket 16, slide glass 17 and pickup groove 11 successively at its top, utilize U-shaped retaining clip 15 that pad 16, slide glass 17 and pickup groove 11 are fixed on the retaining plate 8 at "T"-shaped collection tube 7 mouth of pipe positions, put it in the rotor or rotary head hanging basket of cell centrifugation smearing machine direct centrifugal preparation cell smear then.
According to the design and the method for the invention described above, the device of the application of the invention can be very convenient be collected, formed elements such as the cell in the chorista fluid samples and prepare cell smear fast.Certainly, these apparatus and method also can be used for separating and the smear preparation of cell (bacterium) in cell cultures and the inoculum.
Claims (7)
- A body fluid sample that is used for cytoscopy collect, tripping device, comprise sample collection container and cell smear device, it is characterized in that: said sample collection container comprises that the top is provided with the container body of opening (2) (1), lower sidewall at container body (1) is provided with a horizontal outage (5) that has outage switch (6), the bottom of container body (1) is " V " font or turbination, is provided with "T"-shaped collection tube (7) at " V " font or turbination bottom.
- 2. the body fluid sample that is used for cytoscopy according to claim 1 is collected, tripping device, it is characterized in that: said container body (1) and "T"-shaped collection tube (7) adopt the connection type bag-like container of integrated preparation, and filling orifice (4) is installed on opening (2), and container body (1) top is provided with and is used to the container suspender belt hole (10) that keeps container stable.
- 3. the body fluid sample that is used for cytoscopy according to claim 1 is collected, tripping device, it is characterized in that: said container body (1) is bottle or tank body, "T"-shaped collection tube (7) adopts dismountable mode to be arranged on the bottom of container body (1), the cell smear device that matches with "T"-shaped collection tube (7) comprises a size and common slide glass (17) same fixed groove (11), be provided with the aperture (12) that is used to discharge unnecessary liquid and unloading slide glass (17) in pickup groove (11) bottom, pickup groove (11) upper limb both sides are respectively arranged with two symmetric retaining clip hooks (14) that are used to that the retaining clip open holes (13) of U-shaped retaining clip (15) is installed and lock retaining clip (15), be disposed with slide glass (17) and pad (16) above the pickup groove (11), pad (16) center offer one with the identical through hole of "T"-shaped collection tube (7) openings of sizes, the cell smear device is installed on the "T"-shaped collection tube (7) by U-shaped retaining clip (15).
- 4. the body fluid sample that is used for cytoscopy according to claim 3 is collected, tripping device, and it is characterized in that: the opening (2) at said container body (1) top is provided with the lid (3) that has filling orifice (4).
- 5. the body fluid sample that is used for cytoscopy according to claim 3 is collected, tripping device, it is characterized in that: said "T"-shaped collection tube peristome be provided with one vertical with "T"-shaped collection tube (7) tube wall and with "T"-shaped collection tube (7) mouth of pipe place and same plane, big or small identical with slide glass (17) or be slightly less than the collection tube retaining plate (8) of slide glass (17).
- 6. the body fluid sample that is used for cytoscopy according to claim 3 is collected, tripping device, and it is characterized in that: pan straddle (9) or container top that the bottom of said container body (1) is provided for keeping container stable are provided with container suspender belt hole (10).
- 7. body fluid sample collection, tripping device and a method that is used for cytoscopy is characterized in that, comprises the following steps:1) selects the container body (1) of suitable size according to the amount of pending body fluid, make container body (1) horizontal positioned by pan straddle (9) or container suspender belt hole (10), directly hydrothorax, ascites, urine, cerebrospinal fluid, draining fluids and cell culture fluid and inoculum are joined in the container body (1) from the upper end, perhaps be connected, directly collect body fluid with drainage tube;2) body fluid leaves standstill more than the 20min after joining container body (1), makes that the various formed elementss such as cell in the above-mentioned body fluid sample deposit in the "T"-shaped collection tube (7) of container body (1) bottom " V " font or turbination bottom naturally;3) treat that various formed elements natural subsidence such as cell in the sample are slipped in the "T"-shaped collection tube (7) after, open the outage switch (6) of container body (1) sidewall the body fluid supernatant discharged by outage (5);4), surpass humor collecting container primary treatment ability, then carry out step 1)～3 repeatedly if the body fluid sample amount is big) method collects the formed elementss such as cell in the sample in the "T"-shaped collection tube (7) smoothly;5) after all body fluid sample was finished dealing with, tilt container body (1) was poured out remaining body fluid supernatant;If container body (1) and "T"-shaped collection tube (7) adopt the connection type bag-like container of integrated preparation, then directly cut "T"-shaped collection tube (7) with scissors, re-use common cell smear and prepare apparatus and prepare cell smear;If container body (1) is bottle or tank body, then take off "T"-shaped collection tube (7), add Upper gasket (16), slide glass (17) and pickup groove (11) successively at its top, utilize U-shaped retaining clip (15) pad (16), slide glass (17) and pickup groove (11) to be fixed on the retaining plate (8) at "T"-shaped collection tube (7) mouth of pipe position, put it in the rotor or rotary head hanging basket of cell centrifugation smearing machine direct centrifugal preparation cell smear then.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 201010120630 CN101787345B (en) | 2010-03-10 | 2010-03-10 | Device for collecting and separating body fluid sample used for cell examination and method therefor |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201010120630 CN101787345B (en) | 2010-03-10 | 2010-03-10 | Device for collecting and separating body fluid sample used for cell examination and method therefor |
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| CN101787345A true CN101787345A (en) | 2010-07-28 |
| CN101787345B CN101787345B (en) | 2013-04-17 |
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| CN 201010120630 Expired - Fee Related CN101787345B (en) | 2010-03-10 | 2010-03-10 | Device for collecting and separating body fluid sample used for cell examination and method therefor |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102539211A (en) * | 2012-01-09 | 2012-07-04 | 广州三瑞医疗器械有限公司 | Device and method for making liquid-based cytological smear in one step |
| CN109022281A (en) * | 2018-08-16 | 2018-12-18 | 英诺维尔智能科技（苏州）有限公司 | A kind of novel cell culture vessel |
| TWI661044B (en) * | 2018-10-19 | 2019-06-01 | 薩摩亞商Ｓｃｌ生物科技有限公司 | Culture bottle and culture bottle assembly |
| CN114018667A (en) * | 2015-09-14 | 2022-02-08 | 国立大学法人滋贺医科大学 | Cell-retaining substrate holder for producing observation specimen, kit including same, and method for producing observation specimen |
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| DE3321886C1 (en) * | 1983-06-16 | 1984-05-24 | Dieter Prof. Dr.-Ing. 7500 Karlsruhe Wurz | Method and apparatus for obtaining samples of, in particular, microbial constituents in gas flows |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114018667A (en) * | 2015-09-14 | 2022-02-08 | 国立大学法人滋贺医科大学 | Cell-retaining substrate holder for producing observation specimen, kit including same, and method for producing observation specimen |
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| TWI661044B (en) * | 2018-10-19 | 2019-06-01 | 薩摩亞商Ｓｃｌ生物科技有限公司 | Culture bottle and culture bottle assembly |
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| CN101787345B (en) | 2013-04-17 |
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