WO2012053712A1 - Filter container for collecting cells - Google Patents

Filter container for collecting cells Download PDF

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Publication number
WO2012053712A1
WO2012053712A1 PCT/KR2011/003381 KR2011003381W WO2012053712A1 WO 2012053712 A1 WO2012053712 A1 WO 2012053712A1 KR 2011003381 W KR2011003381 W KR 2011003381W WO 2012053712 A1 WO2012053712 A1 WO 2012053712A1
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filter
container body
cells
container
cell
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PCT/KR2011/003381
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French (fr)
Korean (ko)
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김현창
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주식회사 패스텍
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Priority to CN2011800118633A priority Critical patent/CN102782493A/en
Publication of WO2012053712A1 publication Critical patent/WO2012053712A1/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
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  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention comprises an attachable/detachable coupled structure including an upper portion and a lower portion, which can be separated and assembled, and which can easily separate cells and foreign substances contained in a solution for preserving cells using a membrane member and a filtering member, and easily collect the separated cells on a slide.

Description

세포 채취용 필터 용기Filter container for cell collection
본 발명은 세포 채취용 필터 용기에 관한 것으로, 특히 상,하부 용기가 서로 착탈 가능하도록 결합되고, 서로 다른 메쉬크기를 갖는 막부재와 필터부재를 구비하여 보존 용액내에 함유된 세포의 분리 작업이 용이하게 이루어지도록 그 구조가 개선된 세포 채취용 필터 용기에 관한 것이다.The present invention relates to a filter container for cell collection, and in particular, the upper and lower containers are detachably coupled to each other, and having a membrane member and a filter member having different mesh sizes facilitate separation of cells contained in a preservation solution. The present invention relates to a filter container for cell collection, the structure of which is improved.
일반적으로, 신체 이상 유무를 검사하기 위해 신체 검사 부위에 해당하는 세포를 채취하고, 채취된 세포를 세포 보존액이 담겨진 솔루션통에 보존한 후에, 세포를 슬라이드에 부착시킨 후에 현미경으로 검사하는 방식을 채택하고 있다.In general, to check for abnormalities in the body, cells corresponding to the physical examination site are collected, and the collected cells are preserved in a solution container containing a cell preservation solution, and the cells are attached to a slide and then examined under a microscope. Doing.
종래에는 솔에 묻은 세포를 액에 분산시키기 위한 솔루션통이 별도로 존재하고, 솔루션통을 흔들어서 세포들이 충분히 액중에 퍼진 상태에서 액을 다른 용기에 부어넣고 필터의 아래에 흡입기구를 밀착하여 액을 흡입하고, 필터에 걸러진 세포들을 슬라이드에 문질러 채취하는 방식을 채택하고 있다.Conventionally, there is a separate solution container for dispersing the cells attached to the brush in the liquid, shake the solution container and pour the liquid into another container while the cells are sufficiently spread in the liquid and inhale the liquid by closely attaching the suction device under the filter. In addition, the filter is filtered by rubbing the cells filtered on the slide.
채취막을 슬라이드에 접근시키기 위해 용기와 본체를 분리하면 용기에 남겨진 혼합액들이 중력에 의해 아래로 쏟아져서 소실된다.When the container and the body are separated to bring the sampling membrane to the slide, the mixed liquid left in the container is poured down by gravity and disappeared.
그런데, 솔루션통으로부터 슬라이드로 세포를 옮기는 과정이 일일이 사용자에 의해 수동으로 이루어져야 하기 때문에 시간이 많이 소요되고, 사용자의 손으로부터 슬라이드로 오염물질이나 불순물이 옮겨질 수 있어서 세포검사의 정확성을 떨어뜨릴 수 있었다.However, the process of moving the cells from the solution container to the slides is time-consuming because it must be done manually by the user, and contaminants or impurities can be transferred from the user's hand to the slides, thereby reducing the accuracy of the cell test. there was.
그리고 솔루션통 내에 채취된 세포 외에 이물질이 함유될 수 있으며, 이 경우 이물질과 세포를 분리하기 쉽지 않으므로, 분리를 위한 별도의 도구가 요구된다.In addition, foreign matter may be contained in addition to the cells collected in the solution container, in which case it is not easy to separate the foreign matter and cells, a separate tool for separation is required.
본 발명의 목적은 보존 용액과 채취된 세포를 용이하게 분리시킬 수 있도록 함과 아울러, 분리된 세포를 용이하게 채취할 수 있도록 그 구조가 개선된 세포 채취용 필터 용기를 제공하는 데 있다.SUMMARY OF THE INVENTION An object of the present invention is to provide a filter container for cell harvesting, in which the preservation solution can be easily separated from the collected cells, and the structure of the cell collection can be improved to easily collect the separated cells.
상기한 목적을 달성하기 위한 본 발명은 세포가 담겨진 용액이 수용되도록 상부가 개구된 수용부와, 상기 수용부의 하측에 배치되고 하측으로 개구되도록 형성된 끼움부와, 상기 수용부와 끼움부 사이를 구분하도록 배치되는 막부재로 구성된 상부 용기 본체; 및 상부가 상기 끼움부 내에 수용되도록 착탈 가능하게 결합되고 상부 표면에 상기 막부재를 통과한 세포가 안착되는 필터부와, 상기 필터부의 하측에 배치되어 상기 필터부를 통과하는 용액을 배수하기 위한 공간을 갖는 배수부로 구성된 하부 용기 본체를 구비한 것을 특징으로 한다.The present invention for achieving the above object is to distinguish between the receiving portion and the receiving portion, the upper portion is opened to accommodate the solution containing the cells, the insertion portion formed to be opened to the lower side and the lower portion of the receiving portion, An upper container body composed of a membrane member disposed to be; And a filter part detachably coupled so that an upper part is accommodated in the fitting part and a cell passing through the membrane member on the upper surface thereof, and a space for draining a solution disposed under the filter part and passing through the filter part. It is characterized by including the lower container main body consisting of a drain having.
본 발명은 세포 보존 용액 내에 함유된 세포와 이물질을 간편하게 분리시킬 수 있으며 분리된 세포를 용이하게 채취할 수 있도록 그 구조가 개선된 것인 바, 이에 따르면 본 발명은 상,하부가 서로 분리 및 조립 가능한 착탈식 결합구조로 되어 있으며 막부재와 필터부재를 이용하여 세포 보존 용액 내에 함유된 세포와 이물질을 간편하게 분리시킬 수 있으며, 분리된 세포를 용이하게 슬라이드에 채취할 수 있는 유용한 효과를 갖는다.The present invention can easily separate the cells and foreign matter contained in the cell preservation solution and its structure is improved so that the separated cells can be easily collected, according to the present invention, the upper and lower parts are separated from each other and assembled The detachable coupling structure is possible, and the membrane member and the filter member can be used to easily separate the cells and foreign substances contained in the cell preservation solution, and have a useful effect of easily collecting the separated cells on the slide.
도 1은 본 발명에 따른 세포 채취용 필터 용기의 분해 사시도.1 is an exploded perspective view of a filter container for cell collection according to the present invention.
도 2는 본 발명의 결합상태를 나타낸 사시도.Figure 2 is a perspective view showing a combined state of the present invention.
도 3은 본 발명 필터부를 나타낸 분해 사시도.3 is an exploded perspective view showing the filter unit of the present invention.
도 4는 본 발명의 세포 필터 과정을 나타낸 사용상태도.Figure 4 is a state diagram showing the cell filter process of the present invention.
도 5는 본 발명의 분리된 세포를 슬라이드에 채취하기 전 상태를 나타낸 사용상태도.Figure 5 is a use state showing the state before collecting the separated cells of the present invention on the slide.
이하, 본 발명의 바람직한 실시 예를 첨부된 도면을 참조하여 상세히 설명하기로 한다.Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings.
본 발명에 따른 세포 채취용 필터 용기는, 도 1 내지 도 5를 참조하여 설명하면, 그 구성은 크게 상부 용기 본체(100)와, 상부 용기 본체(100)의 하부에 착탈 가능하게 결합되는 하부 용기 본체(200)로 대별된다.When the filter container for cell collection according to the present invention will be described with reference to Figs. 1 to 5, its configuration is largely the upper container body 100 and the lower container detachably coupled to the lower portion of the upper container body 100. It is roughly divided into the main body 200.
더 상세하게 설명하면, 상부 용기 본체(100)는 세포(C)가 담겨진 용액이 수용되도록 상부가 개구된 수용부(110)와, 수용부(110)의 하측에 배치되고 하측으로 개구되도록 형성된 끼움부(120)와, 수용부(110)와 끼움부(120) 사이를 구분하도록 배치되어 용액 내에 함유된 이물질을 필터링 하기 위한 메쉬구조의 막부재(150)로 구성된다.In more detail, the upper container body 100 has a receiving portion 110 having an upper portion opened to accommodate a solution containing the cells C, and a fitting formed to be opened below the receiving portion 110 and opened downward. It is arranged to distinguish between the portion 120, the receiving portion 110 and the fitting portion 120 is composed of a membrane member 150 of the mesh structure for filtering the foreign matter contained in the solution.
즉, 상부 용기 본체(100)는 중간에 수평으로 배치된 막부재(150)를 기준으로 상,하 측에 수용부(110)와 끼움부(120)가 각각 상,하로 개구된 형태로 형성되어 있다.That is, the upper container body 100 is formed in a shape in which the upper and lower receiving portions 110 and the fitting portion 120 are opened up and down, respectively, based on the membrane member 150 disposed horizontally in the middle. have.
하부 용기 본체(200)는 상부가 끼움부(120) 내에 수용되도록 결합되고 상부 표면에 막부재(150)를 통과한 세포(C)가 안착되는 필터부(210)와, 필터부(210)의 하측에 배치되어 필터부(210)를 통과하는 용액을 배수하기 위한 공간을 갖는 배수부(220)로 구성된다.The lower container body 200 is coupled to be accommodated in the fitting portion 120, the filter portion 210 and the filter portion 210 is seated on the upper surface of the cell (C) passing through the membrane member 150, Is disposed on the lower side is composed of a drain 220 having a space for draining the solution passing through the filter (210).
필터부(210)는 하부 용기 본체(200)의 상부면에 형성되고 다수개의 배수공(212a)이 형성된 요홈 형태의 안착홈(212)과, 안착홈(212)의 상측에 배치되어 막부재(150)를 통과하는 용액과 세포(C)를 분리시켜 세포(C)를 채취하기 위한 메쉬구조의 필터부재(215)와, 필터부재(215)와 안착홈(212) 사이에 배치되어 필터부재(215)를 상측으로 탄성 지지하는 지지부재(216)로 구성된다.The filter unit 210 is formed on the upper surface of the lower container body 200 and is disposed in the groove-shaped seating groove 212 and the seating groove 212 formed with a plurality of drain holes 212a, the membrane member 150 The filter member 215 of the mesh structure for separating the solution (C) and the cell (C) passing through the) and the cell (C) to be collected, and disposed between the filter member 215 and the seating groove 212, the filter member (215) It is composed of a support member 216 for elastically supporting upward.
지지부재(216)은 필터부재(215)를 상측으로 지지함과 아울러, 세포 보존 용액의 배수가 가능하도록 압축 스펀지 소재를 채용할 수 있으나, 이에 한정하지는 아니한다.The support member 216 may support the filter member 215 upwards, and may employ a compressed sponge material to enable drainage of the cell preservation solution, but is not limited thereto.
막부재(150)와 필터부재(215)는 각각 다수개의 구멍을 갖는 메쉬(mesh)구조로 구성되며, 바람직하게는 필터부재(215)의 메쉬 수가 막부재(150)의 메쉬 수보다 많게 형성된다.The membrane member 150 and the filter member 215 each have a mesh structure having a plurality of holes, and preferably, the number of meshes of the filter member 215 is greater than the number of meshes of the membrane member 150. .
이는, 막부재(150)가 세포 보존 용액 내에 함유된 세포(C)를 통과시킴과 아울러 세포 보존 용액 내에 함유된 세포보다 입도가 큰 이물질의 통과를 차단시켜 이물질을 걸러주기 위한 것인 반면에, 필터부재(215)는 막부재(150)를 통과한 세포(C)가 필터부재(215)의 상측에 안착되고 용액만 하부의 배수공(212a)을 통해 외부로 배출시키기 위함이다.While the membrane member 150 passes through the cells C contained in the cell preservation solution, while blocking the passage of the foreign matter having a larger particle size than the cells contained in the cell preservation solution, the foreign matter is filtered out. The filter member 215 is intended to allow the cells C passing through the membrane member 150 to be seated on the upper side of the filter member 215 and to discharge only the solution to the outside through the drain hole 212a at the bottom thereof.
일 예로 막부재(150)의 메쉬 수는 150~300 mesh를 채택하고, 필터부재(215)의 메쉬 수는 500mesh 이상을 채택할 수 있다.For example, the number of meshes of the membrane member 150 may be 150 to 300 mesh, and the number of meshes of the filter member 215 may be 500 mesh or more.
또한, 상,하부 용기 본체(100)(200)는 결합시 상부 용기 본체(100)의 슬립 회전을 방지하기 위한 스톱퍼 수단이 구비된다.In addition, the upper and lower container body 100, 200 is provided with a stopper means for preventing slip rotation of the upper container body 100 at the time of coupling.
스톱퍼 수단은 하부 용기 본체(200)의 외주면에 형성되며 외측으로 돌출된 형태의 걸림돌기(230)와, 걸림돌기(230)와 끼움 결합되도록 상부 용기 본체(100)의 끼움부(120) 내주면에 형성되는 걸림홈부(130)를 구비한 것이다.The stopper means is formed on the outer circumferential surface of the lower container body 200 and is provided on the inner circumferential surface of the fitting portion 120 of the upper container body 100 so as to be engaged with the locking protrusion 230 and the locking protrusion 230. It is provided with a locking groove 130 is formed.
그리고 상,하부 용기 본체(100)(200)는 각각의 외주면에 서로 이격된 다수개의 돌기부(140)(240)가 형성되어 있다.In addition, the upper and lower container bodies 100 and 200 are formed with a plurality of protrusions 140 and 240 spaced apart from each other on each outer circumferential surface.
이는, 상,하부 용기 본체(100)(200)를 각각 클램핑하고 세포(C)를 채취하는 검사장치(미도시됨)의 클램핑 기구와의 클램핑 동작시 파지가 용이하도록 하기 위함이다.This is to facilitate gripping during the clamping operation with the clamping mechanism of the inspection device (not shown) which clamps the upper and lower container bodies 100 and 200 and collects the cells C, respectively.
즉, 클램핑기구가 상,하부 용기 본체(100)(200)의 외주면을 클램핑한 후에 승강 동작시킬 때 유동을 억제시키기 위함이다.That is, the clamping mechanism is for suppressing flow when the lifting operation is performed after clamping the outer peripheral surfaces of the upper and lower container bodies 100 and 200.
이러한 구성을 갖는 본 발명의 작용을 설명하면 다음과 같다.Referring to the operation of the present invention having such a configuration as follows.
본 발명에 따른 세포 채취용 필터 용기는, 상부 용기 본체(100)와 하부 용기 본체(200)의 조립과정을 먼저 설명하면, 상부 용기 본체(100)의 끼움부(120) 내에 하부 용기 본체(200)의 상부를 삽입시키면, 하부 용기 본체(200)의 필터부(210)가 상부 용기 본체(100)의 막부재(150)의 하측에 배치된다.In the filter container for collecting cells according to the present invention, first, the assembling process of the upper container body 100 and the lower container body 200, the lower container body 200 in the fitting portion 120 of the upper container body 100; When the upper portion of the) is inserted, the filter unit 210 of the lower container body 200 is disposed below the membrane member 150 of the upper container body 100.
이때 하부 용기 본체(200)의 걸림돌기(230)는 상부 용기 본체(100)의 내주면에 형성된 걸림홈부(130)에 끼움 결합되어 상부 용기 본체(100)의 슬립 회전을 방지하게 된다.At this time, the locking protrusion 230 of the lower container body 200 is fitted into the locking groove 130 formed on the inner circumferential surface of the upper container body 100 to prevent slip rotation of the upper container body 100.
이러한 조립과정을 거친 본 발명은 상부가 개구된 수용부(110) 측에 솔루션통의 세포 보존 용액을 부으면, 세포 보존 용액 및 세포 보존 용액에 함유된 세포(C)는 막부재(150)를 통과하여 끼움부(120) 내부로 유입되고, 세포 보존 용액에 함유된 이물질은 막부재(150)를 통과하지 못하고 필터링된다.According to the present invention after the assembly process, the cell preservation solution of the solution container is poured on the side of the receiving part 110 having the upper opening. By entering the fitting portion 120, foreign matter contained in the cell preservation solution does not pass through the membrane member 150 is filtered.
이어서, 끼움부(120) 내부로 유입된 용액은 필터부재(215)와 지지부재(216)를 통과하여 배수공(212a)을 통해 배수부(220) 내부로 유입되고, 끼움부(120) 내부로 유입된 세포(C)는 필터부재(215)의 상측에 안착되도록 필터링된다.Subsequently, the solution introduced into the fitting portion 120 passes through the filter member 215 and the supporting member 216 and flows into the draining portion 220 through the drain hole 212a and into the fitting portion 120. The introduced cells C are filtered to be seated on the upper side of the filter member 215.
이후에, 배수부(220) 내로 유입된 용액은 배수부(220)와 연결된 별도의 호스부재를 통해 외부로 배출된다.Thereafter, the solution introduced into the drain 220 is discharged to the outside through a separate hose member connected to the drain 220.
유리 소재의 슬라이드(S)가 슬라이딩 이동되어 필터부재(215)의 상측으로 이송된 후에, 하부 용기 본체(200)가 클램핑 기구를 매개로 상승 동작되면, 필터부재(215)의 상측에 부착된 세포(C)는 슬라이드(S)의 하측면에 부착되면서 채취된다.After the slide S of the glass material is slid and moved to the upper side of the filter member 215, when the lower container body 200 is lifted up through the clamping mechanism, the cells attached to the upper side of the filter member 215 are removed. (C) is taken while being attached to the lower side of the slide (S).
이때, 지지부재(216)는 필터부재(215)를 상측으로 탄성 지지함으로써, 필터부재(215)와 슬라이드(S)의 접촉시 슬라이드(S)를 보호함과 아울러 세포(C)의 부착성을 향상시키는 기능을 수행한다.At this time, the support member 216 elastically supports the filter member 215 upwards, thereby protecting the slide (S) when the filter member 215 and the slide (S) in contact with the adhesion of the cells (C). Perform the function to improve.
상부 용기 본체(100)는 슬라이드(S)의 이송 동작 전에 별도의 클램핑 기구에 의해 상승 동작되어 하부 용기 본체(200)와 분리된다. The upper vessel body 100 is lifted up by a separate clamping mechanism before the transfer operation of the slide S and is separated from the lower vessel body 200.
이후에, 슬라이드(S)의 하측면에 부착된 세포(C)를 현미경으로 관찰하여 채취된 세포(C)의 이상 유무를 검사할 수 있다.Thereafter, the cells (C) attached to the lower side of the slide (S) can be observed under a microscope to check the abnormality of the collected cells (C).
따라서, 본 발명은 상,하부가 서로 분리 및 조립 가능한 착탈식 결합구조로 되어 있으며 막부재(150)와 필터부재(215)를 이용하여 세포 보존 용액 내에 함유된 세포와 이물질을 간편하게 분리시킬 수 있으며, 분리된 세포를 용이하게 슬라이드(S)에 채취할 수 있는 이점을 갖는다.Therefore, the present invention has a removable coupling structure that can be separated and assembled to each other, the upper and lower parts, and can easily separate the cells and foreign matter contained in the cell preservation solution using the membrane member 150 and the filter member 215, The separated cells have an advantage that can be easily collected on the slide (S).

Claims (6)

  1. 세포가 담겨진 용액이 수용되도록 상부가 개구된 수용부와, 상기 수용부의 하측에 배치되고 하측으로 개구되도록 형성된 끼움부와, 상기 수용부와 끼움부 사이를 구분하도록 배치되는 막부재로 구성된 상부 용기 본체; 및An upper container body consisting of a receiving portion having an upper portion opened to accommodate a solution containing cells, a fitting portion disposed below the receiving portion and formed to open downward, and a membrane member disposed to distinguish between the receiving portion and the fitting portion. ; And
    상부가 상기 끼움부 내에 수용되도록 착탈 가능하게 결합되고 상부 표면에 상기 막부재를 통과한 세포가 안착되는 필터부와, 상기 필터부의 하측에 배치되어 상기 필터부를 통과하는 용액을 배수하기 위한 공간을 갖는 배수부로 구성된 하부 용기 본체를 구비한 것을 특징으로 하는 세포 채취용 필터 용기.The filter unit is detachably coupled so that an upper portion is accommodated in the fitting portion, and the cell portion having passed through the membrane member is seated on an upper surface thereof, and a space disposed below the filter portion to drain a solution passing through the filter portion. A cell collection filter container comprising a lower container body composed of a drainage portion.
  2. 청구항 1에 있어서,The method according to claim 1,
    상기 필터부는 상기 하부 용기 본체의 상부면에 형성되고 다수개의 배수공이 형성된 요홈 형태의 안착홈과,The filter unit is formed in the upper surface of the lower container body and the recessed groove of the groove-shaped groove formed with a plurality of drain holes,
    상기 안착홈의 상측에 배치되어 상기 막부재를 통과하는 용액과 세포를 분리시켜 상기 세포를 채취하기 위한 필터부재와,A filter member disposed above the seating groove and separating the solution and the cells passing through the membrane member to collect the cells;
    상기 필터부재와 안착홈 사이에 배치되어 상기 필터부재를 상측으로 탄성 지지하는 지지부재를 구비한 것을 특징으로 하는 세포 채취용 필터 용기.And a support member disposed between the filter member and the seating groove to elastically support the filter member upwardly.
  3. 청구항 1에 있어서,The method according to claim 1,
    상기 상,하부 용기 본체는 결합시 상기 상부 용기 본체의 슬립 회전을 방지하기 위한 스톱퍼 수단을 더 구비한 것을 특징으로 하는 세포 채취용 필터 용기.The upper and lower container body further comprises a stopper means for preventing slip rotation of the upper container body when coupled to the filter container for cell collection.
  4. 청구항 3에 있어서,The method according to claim 3,
    상기 스톱퍼 수단은 상기 하부 용기 본체의 외주면에 형성되며 외측으로 돌출된 형태의 걸림돌기와,The stopper means is formed on the outer circumferential surface of the lower container body and the engaging projection of the form protruding outward,
    상기 걸림돌기와 끼움 결합되도록 상기 상부 용기 본체의 끼움부 내주면에 형성되는 걸림홈부를 구비한 것을 특징으로 하는 세포 채취용 필터 용기.Cell collecting filter container characterized in that it has a locking groove formed on the inner peripheral surface of the fitting portion of the upper container body to be fitted with the locking projection.
  5. 청구항 2에 있어서,The method according to claim 2,
    상기 필터부재와 막부재는 메쉬구조로 구성하되,The filter member and the membrane member is composed of a mesh structure,
    상기 필터부재는 상기 막부재보다 메쉬 수가 더 많은 것을 특징으로 하는 세포 채취용 필터 용기.The filter member is a cell collection filter container, characterized in that the number of mesh more than the membrane member.
  6. 청구항 1에 있어서,The method according to claim 1,
    상기 상,하부 용기 본체는 각각의 외주면에 서로 이격되게 형성된 다수개의 돌기부를 더 구비한 것을 특징으로 하는 세포 채취용 필터 용기.The upper and lower container body is a filter container for cell collection, characterized in that it further comprises a plurality of protrusions formed on each outer peripheral surface spaced apart from each other.
PCT/KR2011/003381 2010-10-19 2011-05-06 Filter container for collecting cells WO2012053712A1 (en)

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