KR101348779B1 - Membrane filter structure and cytology smear method using the same - Google Patents

Abstract

The present invention allows the sample cells contained in the solution to be filtered on the membrane film by sedimentation filtering, and the cushion members are arranged in a vertical stack so as not to interfere with the membrane film. In addition to minimizing the effects of moisture in the process, the membrane filter structure and its structure is improved to form a circular circle in which cells are uniformly distributed without overlapping and denaturation of the cells to allow accurate analysis of the sample cells. It relates to a cell smear method.
In the configuration of the present invention, the through-hole is formed so that the solution solution passes through the membrane body for filtering the sample cell on the upper edge of the through-hole is fixed to the membrane body formed in the upper portion on the lower edge, and the membrane body is lower The hollow is formed to be fitted to the upper outer circumferential surface of the sedimentation guide and the lower guide can enter the inside of the fitting portion when the pressing force is transmitted from the upper, the receiving groove is fitted into the through hole from the lower side of the membrane body and the cushion member is disposed on the upper side A plurality of drainage holes are formed to form the solution solution, and the suction jig is coupled to the lower part.

Description

Membrane filter structure and cytology smear method using the same

The present invention relates to a membrane filter structure and a cell smearing method using the same, and in particular, the sample cells contained in the solution solution are filtered to the membrane film by the sedimentation filtering method, and the cushion member is prevented from interfering with the membrane film. By stacking the bottom layer, it is possible to minimize the influence of moisture in the process of smearing the sample cells on the slide, and to form a circular circle in which cells are uniformly distributed without overlapping and degeneration of cells, thereby accurately analyzing the sample cells. The present invention relates to a membrane filter structure having an improved structure and a cell smearing method using the same.

Generally, to check for physical anomalies, the cells that belong to the physical examination area are collected, the collected cells are stored in a solution bottle containing the cell solution solution, the cells are attached to the slide, and the cells are examined by a microscope .

Conventionally, there is a separate solution box for dispersing the cells on the brush into the solution, and the solution bottle is shaken to pour the solution into the other container while the cells are sufficiently spread in the liquid. The suction device is closely attached to the bottom of the filter, , And the filter cells are rubbed on a slide.

When the container is separated from the main body so as to approach the slides, the liquid mixture remaining in the container is poured down by gravity and is lost.

However, since the process of transferring the cells from the solution bottle to the slide must be performed manually by the user, it takes a lot of time and the contamination or impurities may be transferred from the user's hand to the slide, there was.

In addition, foreign substances may be contained in cells other than the cells collected in the solution container. In this case, since separation of foreign substances and cells is not easy, a separate tool for separation is required.

A prior art for this purpose is disclosed in Korean Patent Registration No. 10-1035427 entitled " Solution container " filed by the present applicant and includes a container body formed so as to open at its upper and lower portions, A filter member disposed inside the container body for filtering the foreign substances contained in the solution solution, and an opening / closing means disposed inside the container body for selectively blocking the inflow of the solution solution will be.

The container body is divided into an upper liquid chamber and a lower liquid chamber communicating with each other, and a receiving member is detachably coupled to the lower portion.

After the solution solution is collected in the lower liquid chamber after being filtered through the filter member, after the receiving member is detached from the container main body, sample cells are applied to the slide using a vacuum adsorber, Has a cumbersome process of smearing through slides through a membrane filter to separate the slides.

That is, conventionally, the process of separating the sample cells from the solution solution and smearing the separated sample cells onto the slide before smearing is cumbersome, and the process of separating the receiving member from the lower part of the container body by using a mechanical device. There is a disadvantage in that workability is reduced because it is required.

On the other hand, the conventional membrane filter has a plurality of drain holes are formed in the upper portion of the filter body, the sponge is seated on the upper portion of the drain hole, the membrane film has a structure fixed to the upper edge of the filter body upper side of the sponge.

The membrane filter forms a vacuum negative pressure at the bottom of the sponge to filter the sample cells on the upper side of the membrane film during the process of draining the solution while inhaling, and then presses the slide on the upper side of the membrane film to form the upper side of the membrane film. In this case, pressure is applied to the sponge side.

However, the conventional membrane filter structure has a disadvantage in that the membrane film is disposed in close contact with the sponge, so that moisture that is unevenly contained in the sponge is formed on the membrane film, and thus cell adsorption is not performed when the slide is smeared. Due to the disadvantage that the cells implanted on the surface of the slide, as shown in Figure 1a, is partially lost in the water-condensed area and only partially adsorbed to have an empty circle in the middle.

In addition, when the density and thickness of the sponge becomes non-uniform in the process of pressing the slide, there is a difference in adhesion between the slides for each part, and as a result, as shown in FIG. As a result, cell denaturation occurs due to cell compression.

To overcome this, when the thickness of the sponge is increased or the edge is fixed while the membrane film is in close contact with the sponge, the pore size of the membrane film in close contact with the sponge becomes large as the membrane film interferes with the sponge. The cells may be lost.

In addition, since the diameter of the conventional membrane film and sponge is fixed, there is a disadvantage that the size of the cell circle implanted on the surface of the slide cannot be changed.

In addition, since the vacuum degree for each part is different in the vacuum suction process described above, overlapping of the cells occurs in a site where a lot of vacuum pressure is applied, or a cell is not located in a part where the vacuum pressure is low, which is not uniform as shown in FIG. 1C. There is a problem of having a cell distribution.

Due to the problems described above, since cell overlap and cell degeneration occur as in the cell photographs of FIGS. 2A and 2B, accurate cell analysis is impossible.

The present invention has been made in view of the above-described problems, and an object thereof is to form a circular circle in which cells are uniformly distributed without overlapping and degeneration of cells in the process of smearing sample cells on a slide. It is to provide a membrane filter structure having an improved structure.

The present invention for achieving the above object is a membrane body having a through-hole so that the solution solution is passed therein and the membrane body is fixed to the upper edge of the through-hole and the upper portion is formed on the lower edge,

A hollow is formed to be fitted to the upper outer circumferential surface of the membrane body, and a settling guide having a lower portion into the fitting portion when a pressing force is transmitted from the upper portion;

The inner side of the membrane body is fitted into the through hole and the receiving groove is formed so that the cushion member is disposed on the upper portion and a plurality of drain holes are formed to pass through the solution solution and the suction jig is coupled to the lower with It features.

The inner kit is disposed such that at least two cushion members are stacked up and down on the upper side of the receiving groove.

The settling guide protrudes around the upper outer circumferential surface to form a stopper for limiting the entry height in the fitting portion.

A locking jaw is formed on the inner circumferential surface of the through hole, and a fixing jaw corresponding to the locking jaw is formed around the outer circumferential surface of the inner kit coupled to the through hole.

It is to limit the entry height when the inner kit is coupled into the through hole of the membrane body.

The upper edge of the membrane body and the edge of the receiving groove are inclined surface is formed so as to narrow the inner diameter from the upper side to the lower side.

The upper surface of the suction jig is formed flat.

Another characteristic element of the present invention is a cell smearing method, by inserting an inner kit disposed so that a plurality of sponges are stacked up and down on a lower side of a membrane body on which a membrane film is fixed, and the membrane film on the upper side of the sponge. Placing them close to each other,

Providing a space formed by the sedimentation guide on the upper side of the membrane film by fitting and coupling the lower one portion of the sedimentation guide to the upper outer circumferential surface of the membrane body;

Coupling a vacuum suction suction jig into the lower side of the inner kit;

Injecting a solution solution containing sample cells into the space;

Settling the sample cells contained in the solution to settle on the upper side of the membrane film;

Draining the solution liquid by suction using the suction jig after the sedimentation of the sample cells is completed;

After the solution solution is drained, the upper end of the settling guide is lowered to a lower position than the membrane film, and the sample cell filtered on the upper side of the membrane film on the lower surface of the slide by lowering the slide on the upper side of the membrane film. Characterized in that it comprises a step of smearing to be implanted.

First, since the method of sedimenting the sample cells in the membrane film is adopted, not only can the sample cells be uniformly distributed, but also have a useful effect of preventing the overlapping of cells.

Second, due to the structural properties of the cushion member laminated up and down, it is possible to minimize the buildup of moisture in the membrane film, it has a useful effect to improve the implantation efficiency of the cells implanted on the slide surface.

Third, by differentially providing the size of the cushion member of a size smaller than the membrane film diameter, there is an advantage that can form a cell circle of the desired size in a customized manner according to the size of the cushion member.

Fourth, since the membrane film can be fixed to the upper edge of the membrane body as a solid without interference with the cushioning member, there is an advantage that can prevent the loss of fine cells by preventing the pore size deformation of the membrane film.

Fifth, as the upper surface of the suction jig is formed in a flat structure, the suction hole and the drain hole are arranged to correspond to each other to provide uniform suction force to the membrane film and the cushion member during vacuum suction, thereby providing uniform drainage force for each part. As a result, rapid drainage is performed and the cells are uniformly distributed by preventing the flow of the sample cells.

Figures 1a, 1b, 1c is a slide photograph showing a cell circle missing, overlapping, unimplanted cell circle.
2A and 2B are enlarged cell photographs showing existing overlapping and denatured cells.
3 is an exploded perspective view of a membrane filter structure according to the present invention.
Figure 4 is a front view of Figure 3;
5 is a combined state of FIG.
Figure 6 is a schematic diagram showing a sequential smearing process of the sample cells using the membrane filter structure of the present invention.
Fig. 7 is a front view showing a slide in which sample cells are implanted by Fig. 6.
8 is a flowchart sequentially showing a method for smearing cells using the membrane filter structure of the present invention.
9 is a photograph showing a slide conceived by the present invention.
10 is an enlarged photograph of normal cells plated by the present invention.

The present invention allows the sample cells contained in the solution to be filtered on the membrane film by the sedimentation filtering method, thereby forming a circular circle in which cells are uniformly distributed without overlapping and degeneration of the cells in the process of smearing the sample cells on the slide. This allows accurate analysis of the sample cells.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

The membrane filter structure according to the present invention will be described with reference to FIGS. 3 to 7, the configuration of which the through hole 105 is formed so that the solution solution S passes therein, and the sample cell at the upper edge of the through hole 105. The membrane body 100 for filtering the edge is fixed and the membrane body 100 is formed with a top portion 110 is formed on the lower edge, and the hollow 205 so that the bottom is fitted to the upper outer peripheral surface of the membrane body 100 When the pressure is transmitted from the upper portion is formed, the lower guide can enter into the interior of the fitting portion 110 and the membrane body 100 is fitted into the through hole 105 from the lower side of the cushion member and the upper cushion member Receiving groove 305 is formed so that the 350 is disposed, a plurality of drain holes 310 is formed so that the solution solution (S) is passed and the inner kit 300 to which the suction jig 400 (suction jig) is coupled to the bottom It consists of.

In more detail, the fitting portion 110 is configured to extend vertically so that the lower portion of the settling guide 200 is formed around the lower outer circumferential surface of the membrane body 100.

The cushion member 350 is preferably disposed as a plurality of upper and lower cushion members 350A and 350B stacked up and down on the upper side of the receiving groove 305. This is to prevent the water of the solution liquid S from directly affecting the membrane film 150.

In addition, the cushion member 350, in consideration of water permeability, for example, it is preferable to employ a sponge material.

The settling guide 200 has a hollow 205 formed at the center thereof so that the upper and lower openings thereof are formed, and a stopper 210 is formed to protrude around the upper outer circumferential surface to limit the entry height of the fitting portion 110.

The size of the hollow 205 has a size corresponding to the diameter of the upper circumferential surface of the membrane body 100.

Coupling process of the sedimentation guide 200 and the membrane body 100 is the primary coupling process for coupling so that the top of the sedimentation guide 200 is located at a higher position than the top of the membrane body 100, the sedimentation guide 200 The lower portion of the fitting portion 110 is fitted into the upper end of the sedimentation guide 200 has a secondary coupling process for coupling to a lower position than the top of the membrane body 100.

Since the top of the sedimentation guide 200 is positioned higher than the membrane film 150 in the first bonding process, the sedimentation guide 200 is combined in a form surrounding the outer circumferential surface of the membrane film 150 to temporarily stop the solution solution S. It forms a space that can be stored.

The inner kit 300 is formed in a radial structure in which a plurality of drainage holes 310 are formed on a pedestal on which the cushion member 350 of the sponge material is seated.

In addition, when the membrane body 100 and the inner kit 300 are coupled, a stopper means for limiting the entry height of the inner kit 300 is provided.

The stopper means is a locking jaw 107 is formed on the upper inner peripheral surface of the through hole 105, the fixing jaw 307 corresponding to the locking jaw 107 is formed around the outer peripheral surface of the inner kit (300).

In addition, the upper edge of the membrane body 100 and the edge of the receiving groove 305 is formed with inclined surfaces 108 and 305a so as to narrow the inner diameter from the upper side to the lower side.

This is to maintain a uniform distribution of the sample cells by preventing the suction force from being concentrated in the center due to the structural characteristics of the drain hole 310 having a radial structure so as to transmit a uniform vacuum suction force for each site.

On the other hand, the suction jig 400 has a drainage structure formed with a plurality of suction holes 405 similar to the drain hole 310 in the upper portion, the suction port 410 is formed therein.

In addition, the suction jig 400 is preferably formed of a flat top surface, which is formed so as to correspond to the lower surface of the drain hole 310 of the inner kit 300, the solution solution (S) passing through the drain hole 310 In order to ensure a constant drainage and uniform drainage of the drainage, the drainage of the sample cells to be deposited on the membrane film 150 is uniformly distributed.

The operation of the present invention having such a configuration will now be described.

In the method for smearing sample cells using the membrane filter structure of the present invention, referring to FIGS. 4 and 6 again, and referring to FIG. 8, first, the inner kit 300 is inserted into the lower side of the membrane body 100. After the lowering, the sedimentation guide 200 is lowered from the upper side of the membrane body 100 so that the upper part of the membrane body 100 into which the inner kit 300 is inserted is fitted into the hollow 205 of the sedimentation guide 200. After coupling, the suction jig 400 is inserted into the inner kit 300 under the inner kit 300.

Coupling process of the sedimentation guide 200 and the membrane body 100 is coupled by the first coupling process for coupling so that the top of the sedimentation guide 200 is located at a higher position than the top of the membrane body 100 as described above do.

At this time, the sedimentation guide 200 is coupled to the upper portion of the membrane body 100 in a form surrounding the outer circumferential surface of the membrane film 150 to form a space for temporarily storing the solution liquid (S).

Subsequently, the solution solution S containing the sample cells is injected into the space using a sample injector 10 (eg, a solution container, a syringe, etc.), and the sample cells contained in the solution solution S are transferred to the membrane film. Settle so as to be seated on the upper side of (150).

After the sample cells are settled on the upper side of the membrane film 150, the solution j is drained by the vacuum suction force using the suction jig 400.

After the solution solution S is drained, the slide 20 is moved from the upper side of the membrane film 150 after the secondary bonding process of lowering the top of the settling guide 200 to a lower position than the membrane film 150. It is lowered and plated so that the filtered sample cells are implanted on the upper side of the membrane film 150 on the lower surface of the slide 20.

At this time, the sedimentation guide 200 can be lowered by the lower pressing force of the slide 20 for lowering the slide 20 from the upper side, or the method of raising the membrane body 100 in a state in which the sedimentation guide 200 is fixed. have.

As the solution solution S is drained through the suction jig 400 through the sedimentation method of the sample cells, the sample cells are uniformly distributed without being biased at a specific site on the membrane film 150, so that the slide 20 The shape of the cell circle C implanted on the surface is implanted into an ideal circular shape (see Fig. 9).

Meanwhile, in the process of implanting the slide 20, air may be blown from the suction jig 400 to the membrane film 150 in the same manner as the conventional process to assist the implantation.

In addition, the present invention can form a cell circle (C) of the desired size in a tailored manner according to the size of the cushion member 350, which is the size of the cushion member 350 of the size smaller than the diameter of the membrane film 150 After being provided differentially, it is possible to form a cell circle (C) of a desired size.

In addition, as the structural properties of the cushion members 350 stacked up and down do not directly affect the membrane film 150, it is possible to minimize the formation of moisture in the membrane film 150. As a result, the implantation efficiency of the cells implanted on the surface of the slide 20 can be improved, which is advantageous in forming the cell circle C on the surface of the slide 20.

In addition, the present invention is different from the conventional method of fixing the membrane filter covering the upper side of the cushioning member 350, for example, because it is fixed directly to the upper edge of the membrane body 100, which is a solid material of synthetic resin, cushion By minimizing the interference with the member 350, it is possible to reduce the pore size variation of the membrane film 150, thereby preventing the loss of the fine cells.

In particular, since the suction jig 400 of the present invention has a flat structure having an upper surface identical to that of the lower surface of the drain hole 310, the suction hole 405 and the drain hole 310 are disposed to correspond to each other, and thus uniform during vacuum suction. The suction force is provided to the membrane film 150 and the cushion member 350 to have a uniform drainage force for each part, so that rapid drainage can be performed and the flow of the sample cells can be prevented to uniformly distribute the cells.

After the slide smearing process is completed, as shown in FIG. 10, which shows an enlarged photograph of the sample cells under a microscope, clear sample cells can be identified without denaturation or overlapping of cells, so that accurate cell analysis can be performed.

The embodiments of the present invention described above can be modified in various ways within the scope and spirit of the present invention.

Description of the Related Art [0002]
10: sample injector 20: slide
100: membrane body 105: through hole
107: engaging jaw 108: inclined surface (of the membrane body)
110: fitting portion 150: membrane film
200: sedimentation guide 205: hollow
210: Stopper 300: Internal Kit
305: receiving groove 305a: inclined plane
307: fixed jaw 310: drain hole
350: cushion member 400: suction jig
405: suction hole 410: suction port
C: Cell Circle S: Solution

Claims (7)

Membrane body 100 having a through-hole 105 to pass through the solution liquid (S) therein, the membrane film 150 is fixed to the upper edge of the through-hole 105 and the fitting portion 110 is formed on the lower edge thereof. Wow,
A hollow 205 is formed to be fitted to the upper outer circumferential surface of the membrane body 100, and the settling guide 200, the lower portion can enter the interior of the fitting portion 110 when the pressing force is transmitted from the upper,
The receiving groove 305 is formed to be fitted into the through hole 105 in the lower side of the membrane body 100 and the cushion member 350 is disposed on the upper portion of the membrane body 100, and a plurality of drain holes so that the solution liquid S passes therethrough ( 310 is formed and the membrane filter structure, characterized in that it has an inner kit 300, the suction jig 400 is coupled to the bottom. The method according to claim 1,
The inner kit 300 is a membrane filter structure, characterized in that arranged on the upper side of the receiving groove 305 at least two or more cushion members 350 are stacked up, down. The method according to claim 1,
The sedimentation guide 200 is protruded around the upper outer circumferential surface of the membrane filter structure, characterized in that a stopper 210 for limiting the entry height in the fitting portion 110 is formed. The method according to claim 1,
Hanging jaw 107 is formed on the inner peripheral surface of the through hole 105,
The fixing jaw 307 corresponding to the locking jaw 107 is formed around the outer circumferential surface of the inner kit 300 coupled to the through hole 105,
Membrane filter structure, characterized in that to limit the entry height when the combination of the inner kit 300 is entered into the through hole 105 of the membrane body (100). The method according to claim 1,
Membrane filter structure, characterized in that the inclined surface (108) (305a) is formed so that the upper edge of the membrane body (100) and the edge of the receiving groove (305) is narrower from the upper side to the lower side. The method according to any one of claims 1 to 5,
Membrane filter structure, characterized in that the upper surface of the suction jig 400 is formed flat. The membrane film 150 is proximate to the upper side of the sponge by inserting an inner kit 300 in which a plurality of sponges are stacked up and down under the membrane body 100 on which the membrane film 150 is fixed. Placing them as possible,
Fitting a lower portion of the settling guide 200 to the upper outer circumferential surface of the membrane body 100 to provide a space formed by the settling guide 200 on an upper side of the membrane film 150;
Coupling the suction jig 400 for vacuum suction into the lower side of the inner kit 300;
Injecting a solution (S) containing sample cells into the space;
Settling the sample cells contained in the solution (S) so as to be seated on the upper side of the membrane film 150;
After the sedimentation of the sample cell is completed, draining the solution (S) by the suction force by using the suction jig 400,
After the solution liquid S is drained, the upper end of the settling guide 200 is lowered to a lower position than the membrane film 150, and the slide 20 is lowered on the upper side of the membrane film 150 to thereby And smearing the filtered sample cells onto the upper side of the membrane film (150) on the lower surface of the slide (20).

Images