CN206308358U - Liquid basal cell chip-detecting apparatus - Google Patents
Liquid basal cell chip-detecting apparatus Download PDFInfo
- Publication number
- CN206308358U CN206308358U CN201621398815.0U CN201621398815U CN206308358U CN 206308358 U CN206308358 U CN 206308358U CN 201621398815 U CN201621398815 U CN 201621398815U CN 206308358 U CN206308358 U CN 206308358U
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- Prior art keywords
- centrifuge tube
- porous
- fixed plate
- fixed
- detecting apparatus
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- 210000000270 basal cell Anatomy 0.000 title claims abstract description 17
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- 238000003780 insertion Methods 0.000 claims abstract description 10
- 230000037431 insertion Effects 0.000 claims abstract description 10
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- 230000000994 depressogenic effect Effects 0.000 claims description 13
- 238000001514 detection method Methods 0.000 abstract description 6
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- 238000011160 research Methods 0.000 abstract description 3
- 238000013399 early diagnosis Methods 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 31
- 238000000034 method Methods 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 3
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- 238000002474 experimental method Methods 0.000 description 2
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- 238000011532 immunohistochemical staining Methods 0.000 description 2
- 238000007901 in situ hybridization Methods 0.000 description 2
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- 208000026435 phlegm Diseases 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
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- 230000009897 systematic effect Effects 0.000 description 2
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- 206010061218 Inflammation Diseases 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
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- 230000009286 beneficial effect Effects 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a kind of liquid basal cell chip-detecting apparatus, including porous centrifuge tube, centrifugation lid and firm banking, porous centrifuge tube ttom of pipe is fixed with multiple regularly arranged Kong Xin, and the bottom of each Kong Xin is provided with the first Elastic flexible glue pad；The lower vertical of porous centrifugation pipe outer wall is formed with two symmetrical boss in the direction of centrifuge tube body；Slide insertion groove is formed with along its length in firm banking, the both sides cell wall restocking above slide insertion groove is provided with fixed plate, the center of fixed plate forms the through hole for only being passed through for porous centrifuge tube；Both sides directly over fixed plate are upwardly formed fixed station respectively, and two fixed stations are set relatively and the opposite face of the two is respectively formed the arc surface being recessed to another side, and the porous centrifuge tube fixing groove being rotatably inserted into for the boss is formed between fixed plate and fixed station.The utility model detection information amount is big, and slice-making quality is high, can be used for the early diagnosis of tumour and scientific research, epidemiology large sample examination.
Description
Technical field
The utility model is related to biocytology detection means, more specifically a kind of liquid basal cell chip-detecting apparatus.
Background technology
Collection and film-making of the liquid-based cell sample manufacturing art to cell are substantially better than Conventional slide, by the cell specimen that will be gathered
Treatment in liquid basal cell reagent treatment is added, the interference of the impurity to film-making such as red blood cell, mucus are can remove, cell is by fully solid
Fixed, film-making is uniform and thin, compared with Traditional papsmear clear background, it is easy to observe, while saving the antigenicity of cell, is exempted from
Epidemic disease histochemical staining, and repeatable film-making, reduce false negative rate, and the recall rate to malignant tumour is higher than Conventional slide technology.
Cell chip can carry out large information capacity to cell, accurately and rapidly screen or detect, realize the mark to different batches
This detects, can save reagent and realize the standardization of experiment condition that reduction systematic error is particularly suited for scientific research large sample simultaneously
Detection and epidemic disease screening.Cell chip preparation process is simple, low cost, it is easy to operate, the used time is short, and detection is without special bar
Part, it is environmentally safe, it is easy to popularization.It is applied widely, can be used for hydrothorax, ascites, urine, vagina or cervical secretions,
The cytology such as phlegm, cerebrospinal fluid are detected, and can carry out various methods such as HE dyeing, pap staining, immunohistochemical staining, in situ hybridization
Detected.
Although liquid-based cell sample manufacturing art and cell chip have above-mentioned various advantages, in clinical detection equal Shortcomings it
Place.Original cell chip（The cell chip prepared using ZL.2005200278140 methods describeds）The dot matrix thickness of making is not
, slice-making quality is easily influenceed by haemocyte in sample and mucus；And though liquid-based cell sample manufacturing art film-making is thin, clear background,
Information content is few, and every slide only detects a sample.
Utility model content
The utility model provides a kind of liquid basal cell chip-detecting apparatus, by original cell chip detection method and liquid
Basal cell's tabletting technology is combined, so as to improve film-making efficiency and slice-making quality.
The utility model is realized according to following technical scheme.
A kind of liquid basal cell chip-detecting apparatus, including porous centrifuge tube, centrifugation lid and under porous centrifuge tube
The firm banking in portion, the porous centrifuge tube inner tube bottom is fixed with multiple regularly arranged Kong Xin, and the bottom of each Kong Xin is equal
It is provided with the first Elastic flexible glue pad；The lower vertical of the porous centrifugation pipe outer wall is formed with two in the direction of centrifuge tube body
Symmetrical boss；Slide insertion groove is formed with along its length in the firm banking, on the slide insertion groove
The both sides cell wall restocking of side is provided with fixed plate, and the center of fixed plate forms the through hole for only being passed through for porous centrifuge tube；Described solid
Both sides directly over fixed board are upwardly formed fixed station respectively, and two fixed stations set relatively and the opposite face of the two is respectively formed to another
The arc surface of face depression, forms the porous centrifuge tube being rotatably inserted into for the boss and fixes between the fixed plate and fixed station
Groove.
The bottom surface of the centrifugation lid is inwardly formed depressed part, and lug boss is formed down again in the top surface of depressed part, described
Diameter of the diameter of lug boss less than depressed part；The second elasticity is provided with the annular top surface that depressed part is cut into by lug boss soft
Rubber cushion.
The height of each Kong Xin is 50mm, a diameter of 3mm in porous centrifuge tube, and hole core spacing is 2mm.
The Kong Xin of porous centrifugation bottom of the tube is opened on slide by the first Elastic flexible glue pad.
The first position sign of porous centrifuge tube is formed with the middle part of the fixed station upper surface；Described porous
The second place indicateing arm corresponding with first position sign is formed with the side wall of the one of Kong Xin in centrifuge tube inside
Know.
The utility model obtains following beneficial effect.
1. the utility model can carry out large information capacity to cell, accurately and rapidly screen or detect, realize to different batches
Sample simultaneously detect, reagent can be saved and the standardization of experiment condition is realized, reduce systematic error, contribute to the sieve of tumour
Look into, early diagnose and scientific research, epidemiology large sample examination.
2. the utility model preparation process is simple, cost is relatively low, it is easy to operate, the used time is short, detects without specific condition,
It is environmentally safe, it is easy to popularization.
3. the utility model is applied widely, and every sample standard deviation that can be made cell suspension can be detected, can be wide
It is general to be detected for the cytology such as hydrothorax, ascites, urine, vagina or cervical secretions, phlegm, cerebrospinal fluid.
4. the utility model has a wide range of application, and can be used for HE dyeing or pap staining, immunohistochemical staining, original position
Hybridization, FISH, In situPCR, oligonucleotides start DNA in situ synthesis etc., can widely with nucleic acid, protein,
The correlation techniques such as cell, tissue are combined, and contribute to examination, early diagnosis and the epidemic disease screening of tumour.
5. each hole of porous centrifuge tube of the present utility model core diameter 3mm, hole core spacing 2mm, more original cell chip
It is 1.5mm per spot diameter, interval 1mm increased, it is to avoid cell overlap phenomenon occur；Hole core height 50mm, it is ensured that cell concentration
It is sufficient.
Each hole core bottom of 6 porous centrifuge tubes of the present utility model is provided with Elastic flexible glue pad, and good leak tightness prevents cell from hanging
Supernatant liquid seepage, it is intact to be made dot matrix form, non-overlapping phenomenon.
7. porous centrifuge tube top of the present utility model is provided with centrifugation lid, prevents cell suspending liquid from dripping, it is ensured that to fill
The cell concentration of foot, and can device to hole core play pressurization, be in close contact itself and slide, further prevent cell suspending liquid from oozing
Leakage.
Brief description of the drawings
Fig. 1 is the structural representation of the porous centrifuge tube of the utility model；
Fig. 2 is the upward view of the porous centrifuge tube of the utility model；
Fig. 3 is the structural representation of the utility model slide firm banking；
Fig. 4 is structural representation of the present utility model.
Wherein：1. porous centrifuge tube；2. lid is centrifuged；
21. depressed parts；22. lug bosses；
23. second Elastic flexible glue pads；3. firm banking；
31. fixed plates；32. fixed stations；
33. slide insertion grooves；34. porous centrifuge tube fixing grooves；
35. through holes；4. boss；
5. hole core；6. the first Elastic flexible glue pad；
7. slide；8. first position sign；
9. second place sign.
Specific embodiment
Below in conjunction with the accompanying drawings and embodiment is further described to the utility model.
As Figure 1-4, a kind of liquid basal cell chip-detecting apparatus, including porous centrifuge tube 1, centrifugation lid 2 and position
In the firm banking 3 of the bottom of porous centrifuge tube 1, it is characterised in that：The inner tube bottom of the porous centrifuge tube 1 is fixed with multiple rules
The hole core 5 of arrangement, the bottom of each hole core 5 is provided with the first Elastic flexible glue pad 6；The bottom of the outer wall of porous centrifuge tube 1
Two symmetrical boss 4 are formed with perpendicular to the direction of centrifuge tube body；It is formed with along its length in the firm banking 3
Slide insertion groove 33, the both sides cell wall restocking in the top of the slide insertion groove 33 is provided with fixed plate 31, fixed plate 31
Center forms the through hole 35 for only being passed through for porous centrifuge tube 1；Both sides directly over the fixed plate 31 are upwardly formed solid respectively
Determine platform 32, two fixed stations 32 set relatively and the opposite face of the two is respectively formed the arc surface being recessed to another side, the fixed plate
The porous centrifuge tube fixing groove 34 being rotatably inserted into for the boss 4 is formed between 31 and fixed station 32.
The bottom surface of the centrifugation lid 2 is inwardly formed depressed part 21, and lug boss is formed down again in the top surface of depressed part 21
22, the diameter of the diameter less than depressed part 21 of the lug boss 22；On the annular top surface that depressed part 21 is cut into by lug boss 22
It is provided with the second Elastic flexible glue pad 23.
The height of each hole core 5 is 50mm, a diameter of 3mm in porous centrifuge tube 1, and hole core spacing is 2mm.
The hole core 5 of the bottom of porous centrifuge tube 1 is opened on slide 7 by the first Elastic flexible glue pad 6.
The first position sign 8 of porous centrifuge tube 1 is formed with the middle part of the upper surface of the fixed station 32；Described
The second place corresponding with first position sign 8 is formed with the side wall of the one of hole core 5 in the inside of porous centrifuge tube 1
Sign 9.
Application method of the present utility model is：
1. the appropriate different samples of collection are added to and be added with accordingly centrifugation, supernatant discarded in the centrifuge tube of separating liquid
Liquid, leaves and takes sediment.
2. the sediment of the different specimens for step 1 being obtained is separately added into base fluid, and mixing is made cell suspending liquid.
3. mark is carried out on slide 7, slide 7 is positioned in slide insertion groove 33；Porous centrifuge tube 1 hangs down
Directly it is put into through hole 35, and by the porous centrifuge tube fixing groove 34 of the boss 4 of its underpart precession firm banking 3 clockwise, makes
The first position sign 8 obtained on fixed station 32 is corresponding with the second place sign 9 on the side wall of hole core 5, finally makes
First Elastic flexible glue pad 6 of the bottom of hole core 5 is closely contacted with slide 7；Centrifugation lid 2 is buckled, makes the mouth of pipe of porous centrifuge tube 1
Contacted with the second Elastic flexible glue pad 23 in centrifugation lid 2.
4. the cell suspending liquid that step 2 is obtained is added separately to carry out film-making in each hole core 5, obtains liquid basal cell core
Piece.
5. the liquid basal cell chip cold acetone made or 95% alcohol fixation 20 minutes, carry out HE dyeing or Pasteur's dye
Color, SABC, in situ hybridization etc., for the cytology examination of tumour.
Separating liquid and base fluid are added in cell suspending liquid preparation process, sick cell can be separated with inflammation, mucus and can
Cell is fully fixed, the form of cell is kept；If sample is courage and uprightness, erythrocyte cracked liquid can be added, destroy the same of red blood cell
When can concentrate other cells to a certain extent, the liquid basal cell chip clear background of preparation, cell concentration is sufficient, is distributed Bao Erjun
It is even, it is easy to observe.
Claims (5)
1. a kind of liquid basal cell chip-detecting apparatus, including porous centrifuge tube（1）, centrifugation lid（2）And positioned at porous centrifugation
Pipe（1）The firm banking of bottom（3）, it is characterised in that：The porous centrifuge tube（1）Inner tube bottom is fixed with multiple regularly arranged
Kong Xin（5）, each Kong Xin（5）Bottom be provided with the first Elastic flexible glue pad（6）；The porous centrifuge tube（1）Outer wall
Lower vertical is formed with two symmetrical boss in the direction of centrifuge tube body（4）；The firm banking（3）It is inside square along its length
To being formed with slide insertion groove（33）, in the slide insertion groove（33）The both sides cell wall restocking of top is provided with fixed plate
（31）, fixed plate（31）Center formed only supply porous centrifuge tube（1）The through hole for passing through（35）；In the fixed plate（31）Just go up
The both sides of side are upwardly formed fixed station respectively（32）, two fixed stations（32）Set relatively and the opposite face of the two is respectively formed to another
The arc surface of face depression, the fixed plate（31）With fixed station（32）Between formed supply the boss（4）What is be rotatably inserted into is porous
Centrifuge tube fixing groove（34）.
2. a kind of liquid basal cell chip-detecting apparatus according to claim 1, it is characterised in that：The centrifugation lid（2）
Bottom surface be inwardly formed depressed part（21）, in depressed part（21）Top surface be formed down lug boss again（22）, the lug boss
（22）Diameter be less than depressed part（21）Diameter；In depressed part（21）By lug boss（22）It is provided with the annular top surface being cut into
Second Elastic flexible glue pad（23）.
3. a kind of liquid basal cell chip-detecting apparatus according to claim 1, it is characterised in that：Porous centrifuge tube（1）It is interior
Each Kong Xin（5）Height be 50mm, a diameter of 3mm, hole core spacing is 2mm.
4. a kind of liquid basal cell chip-detecting apparatus according to claim 1, it is characterised in that：Porous centrifuge tube（1）Bottom
The Kong Xin in portion（5）By the first Elastic flexible glue pad（6）It is opened on slide（7）.
5. a kind of liquid basal cell chip-detecting apparatus according to claim 1, it is characterised in that：In the fixed station（32）
Porous centrifuge tube is formed with the middle part of upper surface（1）First position sign（8）；In the porous centrifuge tube（1）It is internal
One of Kong Xin（5）Side wall on be formed with and first position sign（8）Corresponding second place sign
（9）.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621398815.0U CN206308358U (en) | 2016-12-20 | 2016-12-20 | Liquid basal cell chip-detecting apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621398815.0U CN206308358U (en) | 2016-12-20 | 2016-12-20 | Liquid basal cell chip-detecting apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206308358U true CN206308358U (en) | 2017-07-07 |
Family
ID=59251781
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621398815.0U Expired - Fee Related CN206308358U (en) | 2016-12-20 | 2016-12-20 | Liquid basal cell chip-detecting apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206308358U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107941591A (en) * | 2017-12-28 | 2018-04-20 | 贵州大学 | Small-sized mite class cleaning device |
| CN118329583A (en) * | 2024-03-05 | 2024-07-12 | 上海交通大学医学院附属仁济医院 | A device for preparing multi-sample single cell suspension slide microarray |
-
2016
- 2016-12-20 CN CN201621398815.0U patent/CN206308358U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107941591A (en) * | 2017-12-28 | 2018-04-20 | 贵州大学 | Small-sized mite class cleaning device |
| CN118329583A (en) * | 2024-03-05 | 2024-07-12 | 上海交通大学医学院附属仁济医院 | A device for preparing multi-sample single cell suspension slide microarray |
| CN118329583B (en) * | 2024-03-05 | 2025-10-17 | 上海交通大学医学院附属仁济医院 | Device for preparing multi-sample single-cell suspension slide microarray |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170707 Termination date: 20211220 |
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| CF01 | Termination of patent right due to non-payment of annual fee |