CN113188872B - Cell film-making dyeing all-in-one - Google Patents
Cell film-making dyeing all-in-one Download PDFInfo
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- CN113188872B CN113188872B CN202110580922.4A CN202110580922A CN113188872B CN 113188872 B CN113188872 B CN 113188872B CN 202110580922 A CN202110580922 A CN 202110580922A CN 113188872 B CN113188872 B CN 113188872B
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- 238000004043 dyeing Methods 0.000 title claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 138
- 239000002699 waste material Substances 0.000 claims abstract description 89
- 238000012360 testing method Methods 0.000 claims description 52
- 238000003860 storage Methods 0.000 claims description 16
- 238000010186 staining Methods 0.000 claims description 10
- 238000002360 preparation method Methods 0.000 claims description 4
- 238000012545 processing Methods 0.000 abstract description 7
- 238000002474 experimental method Methods 0.000 abstract description 2
- 238000005406 washing Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 8
- 239000011550 stock solution Substances 0.000 description 8
- 238000005119 centrifugation Methods 0.000 description 6
- 239000000975 dye Substances 0.000 description 5
- 210000000056 organ Anatomy 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 238000001125 extrusion Methods 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 239000010902 straw Substances 0.000 description 4
- 239000006285 cell suspension Substances 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000002380 cytological effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000010827 pathological analysis Methods 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
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- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Centrifugal Separators (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The application relates to a cell slide-making and dyeing integrated machine, which belongs to the field of biological experiment equipment, a waste liquid suction pipe needs to be continuously washed for sucking upper waste liquid by the waste liquid suction pipe, operation steps are increased, the treatment efficiency is reduced, and the following technical scheme is provided for solving the problem that washing is not thorough; the centrifugal cylinder is rotationally connected with the centrifugal groove, and a rotating space for the centrifugal cylinder to rotate is reserved between the centrifugal cylinder and the centrifugal groove; the working table is provided with a waste liquid hopper for receiving waste liquid in the centrifugal cylinder. This application has the realization of being convenient for to the processing of upper waste liquid, improves the treatment effeciency to the upper waste liquid's advantage.
Description
Technical Field
The application relates to the field of biological experiment equipment, in particular to a cell film-making and dyeing all-in-one machine.
Background
With the rapid development of pathological techniques and the improvement of economic development level, the second generation liquid-based cytological flaking technique represented by the sedimentation type liquid-based cytological flaking technique is more and more widely applied to pathological diagnosis, especially to the preparation of cytopathology specimens.
The existing cell slide-making and dyeing integrated machine comprises a workbench and a safety cover, wherein one end of the safety cover is hinged to the workbench, a space can be enclosed by the safety cover and the workbench when the safety cover is closed, and a liquid transfer mechanism, a centrifugal mechanism and a dyeing mechanism are arranged in the space.
The centrifugal mechanism comprises a centrifugal turntable, the centrifugal turntable is arranged on a centrifugal shaft in a penetrating mode, centrifugal units are distributed on the circumference of the centrifugal turntable, when the centrifugal mechanism is used, a test tube containing a reagent is placed on the centrifugal units, the test tube can be driven to rotate in the rotating process of the centrifugal turntable, and the centrifugal effect on the reagent inside the test tube is achieved.
Adopt the waste liquid straw to siphon away the inside upper waste liquid of test tube after the centrifugation is accomplished usually, then drip the dyeing agent in to every centrifugal unit respectively through dyeing mechanism, after the dyeing agent dropwise add is accomplished, control centrifugation carousel rotates for the dyeing agent is full and cell contact dyeing, finally accomplishes the film-making and the dyeing of cell.
When the upper-layer waste liquid is absorbed by the waste liquid suction pipe, the waste liquid suction pipe needs to be continuously washed, so that the operation steps are increased, and the treatment efficiency of the upper-layer waste liquid is reduced; simultaneously to the washing of waste liquid straw when not thorough, the inside upper waste liquid that probably persists one step of waste liquid straw easily causes the pollution to the reagent in the follow-up test tube, is not convenient for realize the processing to the upper waste liquid.
Disclosure of Invention
In order to facilitate the realization to the processing of upper waste liquid, improve the treatment effeciency to the upper waste liquid, this application provides a cell film-making dyeing all-in-one, adopts following technical means:
a cell slice-making and dyeing integrated machine comprises a workbench, a centrifugal turntable arranged on the workbench, a driving piece for driving the centrifugal turntable to rotate, a plurality of centrifugal grooves formed in the edge of the centrifugal turntable and uniformly distributed in the circumferential direction of the centrifugal turntable, and a jacking piece for driving the centrifugal turntable to ascend or descend; a centrifugal cylinder is arranged inside the centrifugal groove and sleeved outside the test tube; the centrifugal cylinder is rotationally connected with the centrifugal groove, and a rotating space for the centrifugal cylinder to rotate is reserved between the centrifugal cylinder and the centrifugal groove; and a waste liquid hopper for receiving waste liquid in the centrifugal cylinder is arranged on the workbench.
Through adopting above-mentioned technical scheme, order about centrifugal carousel through jacking piece and rise, centrifugal carousel can drive centrifuge tube and test tube and rise to the top that the lower extreme of centrifuge tube is located the workstation surface in step, then order about centrifuge tube and overturn around its center of rotation, centrifuge tube can be around its center of rotation to waste liquid fill one side upset, along with centrifuge tube inclination's increase, the upper waste liquid that is located the inside of test tube can flow into waste liquid and fight inside, stir the centrifuge tube of different positions department in proper order, realize the processing to upper waste liquid in the test tube, need not to use the waste liquid straw to carry out the waste liquid extraction, reduced and moved the pollution of getting the waste liquid in-process to reagent, be convenient for realize the processing to upper waste liquid, improve the treatment effeciency to upper waste liquid.
Optionally, a mounting disc is arranged below the centrifugal turntable, a liquid storage cavity is defined between the mounting disc and the centrifugal turntable, the liquid storage cavity is provided with a liquid inlet and a liquid outlet, the liquid storage cavity is communicated with a waste liquid hopper through the liquid inlet, and the liquid outlet is communicated with a liquid drainage channel.
Through adopting above-mentioned technical scheme, through the cooperation of stock solution chamber and flowing back passageway, can realize fighting the processing of collecting the waste liquid in the waste liquid, need not to fight the waste liquid and shift out the workstation, convenient operation, and be located the waste liquid that the waste liquid was fought inside, can in time discharge through stock solution chamber and flowing back passageway, improve the treatment effeciency to the upper waste liquid.
Optionally, the waste liquid hopper is located between the mounting disc and the centrifugal turntable, and the orthographic projection part of the centrifugal groove coincides with the opening at the upper end of the waste liquid hopper.
Through adopting above-mentioned technical scheme, the open end orientation of centrifuge bowl is close to the upset of centrifugal carousel center department for the upper waste liquid of test tube inside gets into waste liquid through the rotation space department between centrifuge bowl and the centrifugal groove and fights inside, realizes the collection to upper waste liquid, through fighting the waste liquid and installing between mounting disc and centrifugal carousel, makes the waste liquid set up the centrifugal motion that does not hinder the centrifuge bowl.
Optionally, be provided with between mounting disc and the centrifugal carousel and accept the carousel, accept and set firmly many spinal branchs vaulting pole between carousel and the centrifugal carousel, many the circumferential direction evenly distributed of centrifugal carousel is followed to the bracing piece.
By adopting the technical scheme, the installation of the waste liquid hopper can be realized by arranging the receiving turntable, and the waste liquid hopper is conveniently installed between the installation disc and the centrifugal turntable; through setting up many spinal branchs vaulting pole, can provide the stable support of centrifugal carousel, keep centrifugal carousel in the horizontality for the centrifugal carousel is difficult to be because of the change of the inside volume agent weight of test tube takes place the slope.
Optionally, a rotating ring is fixedly arranged on one side of the receiving turntable, which faces the mounting disc, the rotating ring is inserted into the liquid storage cavity, and the outer wall of the rotating ring abuts against the inner wall of the liquid storage cavity.
Through adopting above-mentioned technical scheme, peg graft the swivel becket in the inside of stock solution chamber, can realize accepting the sealed of carousel and mounting disc junction for the inside waste liquid of stock solution chamber is difficult for revealing.
Optionally, the driving piece is a stepping motor, and an output end of the stepping motor is fixedly connected with the centrifugal turntable.
Through adopting above-mentioned technical scheme, be convenient for realize the rotation drive to centrifugal turntable, when handling the waste liquid, rotate the centrifuge bowl that corresponds position department earlier, realize the processing to the inside waste liquid of centrifuge bowl, then stir centrifugal turntable and rotate for the centrifuge bowl of keeping away from toggle point position rotates to toggle the department, need not artificial removal and can realize the stirring to centrifuge bowl of different positions department, convenient operation.
Optionally, the waste liquid hopper is funnel-shaped.
Through adopting above-mentioned technical scheme, be convenient for realize the quick discharge to the inside waste liquid of waste liquid fill.
Optionally, a flexible pad is fixedly arranged on one side of the waste liquid hopper facing the centrifugal cylinder.
Through adopting above-mentioned technical scheme, can be when the centrifuge bowl resumes the normal position from centrifugal state, convert the contact between centrifuge bowl and the waste liquid fill into soft contact, reduce the collision between centrifuge bowl and the waste liquid fill.
Optionally, the centrifuge bowl and the test tube can be dismantled and be connected, be provided with the extrusion pad between centrifuge bowl inner wall and the test tube outer wall.
Through adopting above-mentioned technical scheme, fill the interval between centrifuge bowl and the test tube through the dummy block, can be spacing the test tube in the inside of centrifuge bowl for the test tube is difficult for deviating from the centrifuge bowl.
Optionally, the centrifuge tube is a tube body with an opening at the upper end, the test tube is placed inside the centrifuge tube, and the length of the test tube is greater than that of the centrifuge tube.
By adopting the technical scheme, on one hand, the test tube is convenient to mount in the centrifugal cylinder, and the closed end of the centrifugal cylinder can prevent the test tube from being separated from the lower end of the centrifugal cylinder; on the other hand, the length of test tube is greater than the length of centrifuge tube for when centrifuge tube drove the test tube upset, inside the upper waste liquid of test tube was difficult for staying to centrifuge tube.
In summary, the present application has the following beneficial effects:
firstly, a centrifugal turntable is driven to ascend through a jacking piece, the centrifugal turntable can drive a centrifugal cylinder and a test tube to synchronously ascend to the position, above the surface of a workbench, of the lower end of the centrifugal cylinder, then the centrifugal cylinder is driven to overturn around the rotation center of the centrifugal cylinder, the centrifugal cylinder can overturn around the rotation center of the centrifugal cylinder to one side of a waste liquid hopper, and with the increase of the inclination angle of the centrifugal cylinder, upper-layer waste liquid in the test tube can flow into the waste liquid hopper, waste liquid can be extracted without using a waste liquid suction pipe, and the treatment efficiency of the upper-layer waste liquid is improved;
secondly, the centrifugal turntable can be stably supported by the plurality of supporting rods, and the centrifugal turntable is kept in a horizontal state, so that the centrifugal turntable is not easy to incline due to the change of the weight of the content in the test tube;
the third, through set up the dummy block between centrifuge bowl and test tube, can realize dismantling between centrifuge bowl and the test tube and be connected for the test tube is difficult for deviating from centrifuge bowl in centrifugal process.
Drawings
FIG. 1 is a schematic overall structure diagram of a cell slide-making and dyeing integrated machine according to an embodiment of the present application;
FIG. 2 is a schematic diagram showing the connection relationship among a centrifugal turntable, a liquid discharge channel, a stepping motor and a cylinder of the integrated cell slice-making and dyeing machine in the embodiment of the application;
FIG. 3 is a top view of FIG. 2;
FIG. 4 isbase:Sub>A cross-sectional view taken along line A-A of FIG. 3;
FIG. 5 is an enlarged view of portion B of FIG. 4;
FIG. 6 is a schematic diagram of the integrated machine for cell slide-making and staining in the embodiment of the present application showing the connection relationship among the centrifugal turntable, the centrifugal cylinder and the test tube.
In the figure, 11, a workbench; 111. a safety shield; 12. a pipetting mechanism; 13. a dyeing mechanism; 14. a centrifugal mechanism; 141. a centrifugal turntable; 1411. a centrifugal tank; 142. a stepping motor; 143. a centrifugal shaft; 21. installing a disc; 211. a shield ring; 22. carrying the rotary table; 221. a liquid discharge port; 222. a rotating ring; 31. a test tube; 32. a centrifugal cylinder; 321. a rotating shaft; 33. a pressing pad; 4. a support bar; 51. a waste liquid hopper; 511. a flexible pad; 52. a liquid storage cavity; 521. a liquid inlet; 522. a liquid outlet; 53. a liquid discharge channel; 531. a first tube section; 532. an organ pipe section; 533. a second tube section; 6. and a cylinder.
Detailed Description
The present application is described in further detail below with reference to the accompanying drawings.
Referring to fig. 1, the cell slide-making and dyeing integrated machine disclosed in the present application includes a workbench 11, a safety cover 111 is fastened on the workbench 11, one side of the safety cover 111 is hinged to the workbench 11, the other side is a free end, when the safety cover 111 is closed, a space is fastened between the safety cover 111 and the workbench 11, and a liquid-moving mechanism 12, a centrifugal mechanism 14, and a dyeing mechanism 13 are arranged inside the space.
Referring to fig. 1, a staining mechanism 13 and a pipetting mechanism 12 are respectively located at two opposite sides of a centrifugation mechanism 14, and the pipetting mechanism 12 can aspirate cell suspension and release it onto the centrifugation mechanism 14. The centrifugation mechanism 14 can separate the cells in the test tube 31 from the carrier solution and perform an adhesion sheet.
Referring to fig. 1, the staining mechanism 13 respectively drops the staining agent onto the centrifugal mechanism 14, and in the process, the centrifugal mechanism 14 rotates in cooperation with the staining mechanism 13, so that the staining agent is fully contacted with the cells for staining. Finally, the preparation and staining of the cells are completed and the sample can be removed for observation.
With reference to fig. 1 and 2, the centrifugal mechanism 14 includes a centrifugal turntable 141 and a driving member for driving the centrifugal turntable 141 to rotate, the centrifugal turntable 141 is disposed in a disc shape, and the centrifugal turntable 141 is disposed horizontally.
With reference to fig. 3 and 4, the centrifugal turntable 141 is keyed connected to a centrifugal shaft 143 at a center of a circle thereof, the centrifugal shaft 143 is vertically disposed, the driving member is preferably a stepping motor 142, a mounting plate 21 is disposed below the centrifugal turntable 141, the mounting plate 21 and the centrifugal turntable 141 are disposed in parallel and spaced, the stepping motor 142 is mounted on a lower surface of the mounting plate 21, the stepping motor 142 is fixedly connected to the mounting plate 21, an output shaft of the stepping motor 142 penetrates through the center of the mounting plate 21 and is keyed connected to the centrifugal shaft 143, so that the centrifugal shaft 143 can rotate coaxially with the output shaft of the stepping motor 142.
With reference to fig. 3 and 4, a plurality of centrifugal grooves 1411 are formed in the edge of the centrifugal turntable 141, the plurality of centrifugal grooves 1411 are circumferentially distributed in an array manner along the circumferential direction of the centrifugal turntable 141, the cross section of the centrifugal groove 1411 is in a U-shaped configuration, the open end of the centrifugal groove 1411 is disposed outwards, the distance between the two side walls of the centrifugal groove 1411 is gradually reduced from inside to outside, and the test tube 31 is mounted inside the centrifugal groove 1411.
Referring to fig. 2 and 4, the test tube 31 is detachably coupled to the centrifuge tank 1411. The inside of every centrifugal groove 1411 all is provided with centrifuge tube 32, and centrifuge tube 32 is the cylindric setting that has the upper end open-ended, and test tube 31 places in the inside of centrifuge tube 32, and the length of test tube 31 is greater than the length of centrifuge tube 32, and when test tube 31 placed inside centrifuge tube 32, the open end of test tube 31 exposes in the outside of centrifuge tube 32, is convenient for realize taking to test tube 31.
With reference to fig. 4 and 5, the inner peripheral wall of the centrifuge tube 32 is adhered with the extrusion pad 33, the extrusion pad 33 is made of silica gel, and the extrusion pad 33 can fill the gap between the inner wall of the centrifuge tube 32 and the test tube 31, so that the test tube 31 is not easy to separate from the centrifuge tube 32, and the detachable connection between the centrifuge tube 32 and the test tube 31 is realized.
Referring to fig. 5 and 6, a rotating shaft 321 is disposed between the centrifugal cylinder 32 and the centrifugal trough 1411, an axis of the rotating shaft 321 is perpendicular to a depth direction of the centrifugal trough 1411, the centrifugal cylinder 32 is rotatably connected to the centrifugal trough 1411 through the rotating shaft 321, a rotating space is reserved between an inner wall of the centrifugal trough 1411 and the centrifugal cylinder 32, so that the centrifugal cylinder 32 can turn inwards or outwards around the rotating shaft 321, and when the centrifugal turntable 141 performs centrifugal motion, the centrifugal cylinder 32 can turn outwards around the rotating shaft 321.
With reference to fig. 4 and 5, a receiving turntable 22 is disposed between the centrifugal turntable 141 and the mounting plate 21, the receiving turntable 22 is disposed in a disc shape, and the receiving turntable 22 and the centrifugal turntable 141 are disposed in parallel and spaced apart from each other.
Combine fig. 2 and fig. 4, the edge of accepting the carousel 22 upper surface has set firmly many spinal branchs vaulting pole 4, many spinal branchs vaulting pole 4 are circumference array distribution along the circumferential direction of accepting carousel 22, and bracing piece 4 and centrifugal groove 1411 set up mutually in a staggered manner, the upper end of bracing piece 4 and centrifugal carousel 141 lower fixed surface connection, the lower extreme of bracing piece 4 and accept carousel 22 upper fixed surface connection, can provide the stable support of centrifugal carousel 141, make centrifugal carousel 141 difficult emergence crooked.
With reference to fig. 4 and 5, a waste liquid hopper 51 is fixedly mounted at the center of the upper surface of the receiving turntable 22, the waste liquid hopper 51 is arranged in a funnel shape, the axis of the waste liquid hopper 51 coincides with the axis of the centrifugal shaft 143, the orthographic projection portion of the centrifugal groove 1411 coincides with the upper end opening of the waste liquid hopper 51, and when the opening end of the centrifugal cylinder 32 rotates inward, the upper layer of waste liquid in the test tube 31 can enter the waste liquid hopper 51 through the gap between the centrifugal groove 1411 and the centrifugal cylinder 32.
Referring to fig. 4 and 5, a flexible pad 511 is bonded to the outer peripheral wall of the waste liquid bucket 51, and the flexible pad 511 is made of corrosion-resistant rubber, and is capable of converting the contact between the centrifuge bowl 32 and the waste liquid bucket 51 into soft contact when the centrifuge bowl 32 is restored from the centrifugation state, thereby reducing the collision between the centrifuge bowl 32 and the waste liquid bucket 51.
With reference to fig. 4 and 5, a liquid storage cavity 52 is defined between the receiving rotary disc 22 and the mounting disc 21. The receiving turntable 22 is provided with a liquid outlet 221 at the overlapping position of the receiving turntable and the waste liquid bucket 51, the liquid storage cavity 52 is provided with a liquid inlet 521 and a liquid outlet 522, the liquid outlet 221 is communicated with the liquid inlet 521, the liquid storage cavity 52 is communicated with the waste liquid bucket 51 through the liquid outlet 221, and waste liquid in the waste liquid bucket 51 can enter the liquid storage cavity 52 through the liquid outlet 221.
Referring to fig. 1 and 4, the liquid storage cavity 52 is communicated with a liquid discharge channel 53 at the liquid outlet 522 thereof, the liquid discharge channel 53 includes a first pipe section 531, an organ pipe section 532 and a second pipe section 533, the first pipe section 531, the organ pipe section 532 and the second pipe section 533 are communicated, the organ pipe section 532 is fixedly disposed between the first pipe section 531 and the second pipe section 533, wherein the first pipe section 531 is fixedly connected with the liquid outlet 522, and one end of the second pipe section 533 away from the organ pipe section 532 extends to the outside of the workbench 11 and is communicated with a municipal drainage pipeline.
Combine fig. 4 and 5, the lower surface edge of accepting carousel 22 is provided with swivel 222, swivel 222 with accept carousel 22 integrated into one piece and set up, the lower terminal surface butt of swivel 222 in the edge of mounting disc 21 upper surface, the mounting disc 21 has sheltered from ring 211 in its edge is upwards buckled, shelter from the outside that ring 211 cover was located swivel 222, swivel 222 outer wall butt in stock solution chamber 52 inner wall this moment, cooperation through swivel 222 and sheltering from ring 211 realizes the sealed to stock solution chamber 52.
With reference to fig. 4 and 5, the inside of the working table 11 is fixedly provided with a jacking member for driving the centrifugal turntable 141 to ascend or descend, the jacking member is a cylinder 6, the cylinder 6 is vertically arranged, the telescopic end of the cylinder 6 is fixedly connected with the lower surface of the mounting disc 21, the number of the cylinders 6 is two, the two cylinders 6 are symmetrically arranged by taking the circle center of the mounting disc 21 as the center, after the slide manufacturing is completed, the two cylinders 6 are controlled to simultaneously jack the mounting disc 21 until the centrifugal cylinder 32 is completely located outside the working table 11, and then the centrifugal cylinder 32 is respectively shifted, so that the centrifugal cylinder 32 rotates inwards around the rotation center of the centrifugal cylinder 32, and the waste liquid inside the centrifugal cylinder 32 is dumped.
The implementation principle of the embodiment is as follows: absorb cell suspension through moving liquid mechanism 12, and release to the inside of test tube 31, at the in-process that moves liquid, centrifugal carousel 141 cooperation moves liquid mechanism 12 and rotates, so that cell suspension can place respectively in each test tube 31, control centrifugal shaft 143 rotates, and drive centrifugal carousel 141 and rotate, make the cell in the test tube 31 with bear the weight of the solution separation, and realize adhering to the film-making, the dyeing agent drips and puts the back that finishes, control centrifugal carousel 141 rotates, make the dyeing agent fully with cell contact dyeing, the film-making and the dyeing of final completion cell.
After accomplishing, two cylinders 6 of control are jacking mounting disc 21 simultaneously until centrifuge tube 32 is located the outside of workstation 11 completely, then stir centrifuge tube 32 respectively, make centrifuge tube 32 inwards rotate around its center of rotation, upper waste liquid in test tube 31 can get into inside waste liquid fill 51 through the interval department between centrifuge bowl 1411 and centrifuge tube 32, the inside waste liquid of waste liquid fill 51 can enter into the inside of stock solution chamber 52 through leakage fluid dram 221, the back shifts out through the liquid outlet 522 and the drainage channel 53 of stock solution chamber 52, the realization is to empting of the inside waste liquid of centrifuge tube 32.
The embodiments of the present invention are preferred embodiments of the present application, and the scope of protection of the present application is not limited by the embodiments, so: equivalent changes in structure, shape and principle of the present application shall be covered by the protection scope of the present application.
Claims (5)
1. A cell slide-making and dyeing integrated machine comprises a workbench (11), a centrifugal turntable (141) arranged on the workbench (11) and a driving piece for driving the centrifugal turntable (141) to rotate, wherein a plurality of centrifugal grooves (1411) are formed in the edge of the centrifugal turntable (141), and the plurality of centrifugal grooves (1411) are uniformly distributed in the circumferential direction of the centrifugal turntable (141), and is characterized by further comprising a jacking piece for driving the centrifugal turntable (141) to ascend or descend; a centrifugal cylinder (32) is arranged inside the centrifugal groove (1411), and the centrifugal cylinder (32) is sleeved outside the test tube (31); the centrifugal cylinder (32) is rotationally connected with the centrifugal groove (1411), and a rotating space for the centrifugal cylinder (32) to rotate is reserved between the centrifugal cylinder (32) and the centrifugal groove (1411); a waste liquid hopper (51) for receiving waste liquid in the centrifugal cylinder (32) is arranged on the working table (11);
a mounting disc (21) is arranged below the centrifugal turntable (141), a liquid storage cavity (52) is defined between the mounting disc (21) and the centrifugal turntable (141), a liquid inlet (521) and a liquid outlet (522) are arranged in the liquid storage cavity (52), the liquid storage cavity (52) is communicated with a waste liquid hopper (51) through the liquid inlet (521), and a liquid drainage channel (53) is communicated with the liquid outlet (522);
the waste liquid hopper (51) is positioned between the mounting disc (21) and the centrifugal turntable (141), and the orthographic projection part of the centrifugal groove (1411) is superposed with an opening at the upper end of the waste liquid hopper (51);
the centrifugal cylinder (32) is detachably connected with the test tube (31), and a squeezing pad (33) is arranged between the inner wall of the centrifugal cylinder (32) and the outer wall of the test tube (31);
a bearing turntable (22) is arranged between the mounting disc (21) and the centrifugal turntable (141), a plurality of support rods (4) are fixedly arranged between the bearing turntable (22) and the centrifugal turntable (141), and the support rods (4) are uniformly distributed along the circumferential direction of the centrifugal turntable (141);
one side of the receiving turntable (22) facing the mounting disc (21) is fixedly provided with a rotating ring (222), the rotating ring (222) is inserted into the liquid storage cavity (52), and the outer wall of the rotating ring (222) abuts against the inner wall of the liquid storage cavity (52).
2. A cell slide-making and dyeing integrated machine according to claim 1, wherein the driving member is a stepping motor (142), and the output end of the stepping motor (142) is fixedly connected with the centrifugal turntable (141).
3. An integrated machine for cell slide preparation and staining as claimed in claim 1, wherein the waste liquid hopper (51) is funnel-shaped.
4. An integrated machine for cell slide preparation and staining as claimed in claim 1, wherein a flexible pad (511) is fixed on one side of the waste liquid hopper (51) facing the centrifugal cylinder (32).
5. An integrated machine for cell slice-making and dyeing according to claim 1, characterized in that the centrifuge tube (32) is a tube body with an upper end opening, the test tube (31) is placed inside the centrifuge tube (32), and the length of the test tube (31) is longer than the length of the centrifuge tube (32).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110580922.4A CN113188872B (en) | 2021-05-26 | 2021-05-26 | Cell film-making dyeing all-in-one |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110580922.4A CN113188872B (en) | 2021-05-26 | 2021-05-26 | Cell film-making dyeing all-in-one |
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| Publication Number | Publication Date |
|---|---|
| CN113188872A CN113188872A (en) | 2021-07-30 |
| CN113188872B true CN113188872B (en) | 2023-01-31 |
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| CN202110580922.4A Active CN113188872B (en) | 2021-05-26 | 2021-05-26 | Cell film-making dyeing all-in-one |
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| CN114453146B (en) * | 2022-04-12 | 2022-06-14 | 深圳人体密码基因科技有限公司 | Centrifugal device for gene detection of self-separation solution |
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