CN113188871B - Cell film-making dyeing all-in-one with liquid structure falls - Google Patents
Cell film-making dyeing all-in-one with liquid structure falls Download PDFInfo
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- CN113188871B CN113188871B CN202110579662.9A CN202110579662A CN113188871B CN 113188871 B CN113188871 B CN 113188871B CN 202110579662 A CN202110579662 A CN 202110579662A CN 113188871 B CN113188871 B CN 113188871B
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- 239000007788 liquid Substances 0.000 title claims abstract description 117
- 238000004043 dyeing Methods 0.000 title claims abstract description 23
- 239000002699 waste material Substances 0.000 claims abstract description 74
- 238000012360 testing method Methods 0.000 claims abstract description 58
- 238000009434 installation Methods 0.000 claims abstract description 10
- 230000007246 mechanism Effects 0.000 claims description 25
- 238000005119 centrifugation Methods 0.000 claims description 14
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 14
- 239000010902 straw Substances 0.000 abstract description 12
- 238000012545 processing Methods 0.000 abstract description 8
- 238000005406 washing Methods 0.000 abstract description 3
- 238000002474 experimental method Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 13
- 238000010186 staining Methods 0.000 description 9
- 230000008569 process Effects 0.000 description 6
- 238000003825 pressing Methods 0.000 description 5
- 238000003756 stirring Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000001125 extrusion Methods 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000001360 synchronised effect Effects 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000002380 cytological effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000010827 pathological analysis Methods 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- Immunology (AREA)
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Abstract
The application relates to a cell slide-making and dyeing integrated machine with a liquid pouring structure, belongs to the field of biological experiment equipment, and provides the following technical scheme aiming at the problem of reagent pollution in a test tube caused by incomplete washing of a waste liquid suction pipe; the workstation is cavity setting, and the inside of workstation has set firmly the mount pad, and the mount pad has been seted up and has been had upper end open-ended installation cavity, and the inside of installation cavity is provided with the slurcam, and slurcam and test tube outer wall are tangent, and the mount pad setting can order about the slurcam and promote centrifuge bowl around its central pivoted promotion structure. This application has the influence that reduces the waste liquid straw to the inside reagent of test tube, realizes the advantage to the processing of upper waste liquid.
Description
Technical Field
The application relates to the field of biological experiment equipment, in particular to a cell film-making and dyeing all-in-one machine with a liquid pouring structure.
Background
With the rapid development of pathological techniques and the improvement of economic development level, the second generation liquid-based cytological flaking technique represented by the sedimentation type liquid-based cytological flaking technique is more and more widely applied to pathological diagnosis, especially to the preparation of cytopathology specimens.
After adopting cell film-making dyeing all-in-one to the reagent centrifugation, adopt the waste liquid straw to siphon away the inside upper waste liquid of test tube through needs, when adopting the waste liquid straw to absorb upper waste liquid, need continuous washing waste liquid straw, because the washing of waste liquid straw is not thorough, the upper waste liquid in the last step test tube may remain in the inside of waste liquid straw, when using the reagent in the follow-up test tube of the waste liquid straw absorption that does not wash thoroughly, cause the pollution to reagent in the test tube easily.
Disclosure of Invention
In order to reduce the influence of waste liquid straw to the inside reagent of test tube, realize the processing to upper waste liquid, this application provides the cell film-making dyeing all-in-one with down the liquid structure, adopts following technical means:
a cell slide-making and dyeing integrated machine with a liquid pouring structure comprises a workbench and a centrifugal mechanism, wherein the centrifugal mechanism comprises a centrifugal turntable and a driving piece for driving the centrifugal turntable to rotate, a plurality of centrifugal grooves are formed in the edge of the centrifugal turntable and are uniformly distributed along the circumferential direction of the centrifugal turntable, a centrifugal cylinder is rotatably arranged in each centrifugal groove, and the centrifugal cylinder is sleeved outside a test tube;
the workstation is cavity setting, the inside of workstation has set firmly the mount pad, the installation cavity that has upper end open-ended is seted up to the mount pad, the inside of installation cavity is provided with the slurcam, slurcam and test tube outer wall are tangent, the mount pad sets up and can orders about the slurcam and promote centrifuge tube around its central pivoted promotion structure.
Through adopting above-mentioned technical scheme, place the test tube that will hold reagent on centrifugal carousel, centrifugal carousel pivoted in-process can drive centrifuge tube and its inside test tube synchronous rotation, realizes the centrifugal action to the inside reagent of test tube. After the centrifugation is accomplished, order about the slurcam through promoting the structure and promote the cylinder and overturn around its rotation center, make along with the inclination increase of cylinder, the upper waste liquid that is located the inside in test tube can flow through the opening of cylinder, realize the processing to the upper waste liquid in the test tube, need not to use the waste liquid straw to absorb the upper waste liquid, the reduction moves the contact of getting between the different inside reagents of test tube of upper waste liquid in-process, and then reduce the influence of waste liquid straw to the inside reagent of test tube, the realization is to the processing of upper waste liquid.
Optionally, the pushing structure includes a telescopic pushing member, a track groove is formed in the side wall of the mounting cavity, two ends of the pushing plate are respectively inserted into the track groove, and the telescopic pushing member can drive the pushing plate to slide along the track groove.
By adopting the technical scheme, when the telescopic pushing piece extends, the telescopic pushing piece can push the pushing plate to move along the track groove, and the pushing plate is tangent to the outer wall of the centrifugal cylinder, so that the centrifugal cylinder can be pushed to rotate around the rotation center of the centrifugal cylinder in the moving process of the pushing plate, and the centrifugal cylinder is further pushed to rotate.
Optionally, the telescopic pushing member is a cylinder, one end of the cylinder is hinged to the bottom wall of the mounting cavity, and the other end of the cylinder is hinged to the pushing plate.
Through adopting above-mentioned technical scheme, through the cylinder, can realize the push-and-pull effect to the slurcam for the slurcam can follow the back and forth movement of orbit groove. Because the centrifugal cylinder rotates around the rotation center, the movement track of the free end of the centrifugal cylinder is arc-shaped, and the two ends of the air cylinder are hinged, so that the inclination angle of the air cylinder is gradually changed in the pushing process, the movement track of the pushing plate is the same as the movement track of the free end of the centrifugal cylinder, and the tangential state of the pushing plate and the centrifugal cylinder is convenient to maintain.
Optionally, a push rod is arranged on one side of the push plate facing the centrifugal cylinder, the push rod comprises two clamping portions, and the two clamping portions are respectively arranged on two sides of the centrifugal cylinder.
By adopting the technical scheme, the centrifugal cylinder can be limited between the two clamping parts when the push rod pushes the centrifugal cylinder, and then the centrifugal cylinder is not easy to deflect in the process of pushing the centrifugal cylinder to rotate, so that the stability of the centrifugal cylinder in the rotating process is improved.
Optionally, the number of the push rods is multiple, and the multiple push rods are arranged at equal intervals along the length direction of the push plate.
Through adopting above-mentioned technical scheme, through setting up a plurality of push rods, correspond push rod and centrifuge bowl, be convenient for realize the synchronous promotion to a plurality of centrifuge bowls, improve the cleaning efficiency to the upper waste liquid.
Optionally, a waste liquid hopper for receiving waste liquid in the centrifugal cylinder is arranged in the mounting cavity, and the orthographic projection part of the centrifugal groove is overlapped with an opening at the upper end of the waste liquid hopper.
Through adopting above-mentioned technical scheme, the open end orientation of centrifuge bowl is close to waste liquid fill department upset for inside upper waste liquid of test tube gets into waste liquid fill through the interval department between centrifuge bowl and the centrifuge bowl, realizes the collection to upper waste liquid.
Optionally, a liquid discharge port is formed in the bottom wall of the waste liquid bucket, the liquid discharge port is communicated with a liquid discharge pipeline, and the liquid discharge pipeline extends to the outside of the workbench.
Through adopting above-mentioned technical scheme, the inside upper waste liquid of waste liquid fill can be through the inside of flowing back pipeline of leakage fluid dram discharge to arrange to the outside of workstation through flowing back pipeline, improve the treatment effeciency to the upper waste liquid.
Optionally, the driving piece is a stepping motor, and an output end of the stepping motor is fixedly connected with the centrifugal turntable.
Through adopting above-mentioned technical scheme, be convenient for realize the rotation drive to centrifugal turntable, when handling the waste liquid, rotate the centrifuge bowl that corresponds position department earlier, realize the processing to the inside waste liquid of centrifuge bowl, then stir centrifugal turntable and rotate for the centrifuge bowl of keeping away from stir point position rotates to stirring the department, need not artificial removal and can realize stirring convenient operation to the centrifuge bowl of different positions department.
Optionally, an extrusion pad is arranged between the inner wall of the centrifuge bowl and the outer wall of the test tube, and the extrusion pad is fixedly connected with the centrifuge bowl.
Through adopting above-mentioned technical scheme, fill the interval between centrifuge bowl and the test tube through the extrusion pad, can be spacing in the inside of centrifuge bowl with the test tube for the test tube is difficult for deviating from the centrifuge bowl.
Optionally, the centrifuge tube has a tube body with an upper end opening, and the length of the test tube is greater than that of the centrifuge tube.
By adopting the technical scheme, on one hand, the test tube is convenient to mount in the centrifugal cylinder, and the closed end of the centrifugal cylinder can prevent the test tube from being separated from the lower end of the centrifugal cylinder; on the other hand, the length of test tube is greater than the length of centrifuge tube for when centrifuge tube drove the test tube upset, inside the upper waste liquid of test tube was difficult for staying to centrifuge tube.
In summary, the present application has the following beneficial effects:
first, through set up the centrifuge bowl on centrifugal mechanism, centrifuge bowl and centrifugal mechanism rotate and connect, order about the catch plate through promoting the structure and promote the centrifuge bowl around its rotation center upset, make along with the inclination increase of centrifuge bowl, the upper waste liquid that is located the inside in test tube can flow through the opening of centrifuge bowl, realize the processing to the upper waste liquid in the test tube, need not to use the waste liquid straw to absorb the upper waste liquid, the reduction is moved and is got the contact between the different inside reagents in test tube of upper waste liquid in-process, and then reduce the influence of waste liquid straw to the inside reagent of test tube, the realization is to the processing of upper waste liquid.
Secondly, a plurality of push rods are arranged on the push plate, so that synchronous pushing of a plurality of centrifugal cylinders is facilitated, and the cleaning efficiency of upper-layer waste liquid is improved;
the third, through setting up waste liquid fill and flowing back pipeline, be convenient for realize shifting out the workstation with upper waste liquid to the collection and the processing of upper waste liquid, improve the treatment effeciency to the upper waste liquid.
Drawings
FIG. 1 is a schematic diagram of the overall structure of a cell slide-making and dyeing integrated machine with a liquid pouring structure in the embodiment of the application;
FIG. 2 is a top view of the connection relationship among the centrifugal turntable, the mounting seat, the pushing structure and the centrifugal cylinder in the integrated machine for preparing and dyeing cell sheets with a liquid pouring structure according to the embodiment of the present application;
FIG. 3 isbase:Sub>A cross-sectional view taken along line A-A of FIG. 2;
FIG. 4 is a schematic diagram of the cell slide-making and staining integrated machine with a liquid pouring structure showing the connection relationship among the centrifugal turntable, the centrifugal cylinder and the test tube according to the embodiment of the present application;
FIG. 5 is a schematic diagram illustrating the connection relationship among the centrifugal turntable, the mounting seat, the pushing structure and the centrifugal cylinder in the integrated machine for cell slide preparation and staining with a liquid pouring structure according to the embodiment of the present application;
fig. 6 is an enlarged view of a portion B in fig. 5.
In the figure, 1, a workbench; 11. a safety shield; 2. a pipetting mechanism; 3. a centrifugal mechanism; 31. a centrifugal turntable; 311. a centrifugal tank; 32. a stepping motor; 33. a centrifugal shaft; 4. a dyeing mechanism; 51. a centrifugal cylinder; 511. a rotating shaft; 52. a test tube; 53. pressing the cushion; 6. a mounting base; 61. a mounting cavity; 62. a track groove; 63. a liquid discharge port; 7. a push plate; 71. a round bar section; 72. a square plate section; 8. a pushing structure; 81. a push rod; 811. a clamping portion; 812. a rigid connection; 82. a cylinder; 821. a cylinder body; 822. a telescopic rod; 83. a rotating ring; 9. a waste liquid hopper; 10. a liquid discharge pipeline.
Detailed Description
The present application is described in further detail below with reference to figures 1-6.
Referring to fig. 1, for the cell film-making dyeing all-in-one with structure of falling liquid that this application discloses, including workstation 1, the lock has safety guard 11 on the workstation 1, and one side of safety guard 11 and workstation 1 are articulated, and the opposite side is the free end when safety guard 11 closes, and the lock becomes the space between safety guard 11 and the workstation 1, and the inside in this space is provided with moves liquid mechanism 2, centrifugal mechanism 3, dyeing mechanism 4.
Referring to fig. 1, a staining mechanism 4 and a pipetting mechanism 2 are respectively located at two opposite sides of a centrifugation mechanism 3, and the pipetting mechanism 2 can suck cell suspension and release the cell suspension onto the centrifugation mechanism 3. The centrifugal mechanism 3 can separate the cells in the test tube 52 from the carrier solution and realize the adhesion slide.
Referring to fig. 1, the centrifugal mechanism 3 is respectively dropped with a staining agent by the staining mechanism 4, and in this process, the centrifugal mechanism 3 rotates in cooperation with the staining mechanism 4, so that the staining agent is sufficiently contacted with the cells for staining. Finally, the preparation and staining of the cells are completed and the sample can be removed for observation.
With reference to fig. 2 and 3, the centrifugal mechanism 3 includes a centrifugal turntable 31 and a driving member for driving the centrifugal turntable 31 to rotate, the centrifugal turntable 31 is disposed in a disc shape, and the centrifugal turntable 31 is disposed horizontally.
With reference to fig. 2 and 3, the centrifugal turntable 31 is keyed with a centrifugal shaft 33 at a center of the centrifugal turntable, the centrifugal shaft 33 is vertically disposed, the driving member is preferably a stepping motor 32, and the stepping motor 32 and the centrifugal shaft 33 rotate coaxially, so as to drive the centrifugal turntable 31 to rotate.
With reference to fig. 2 and 3, a plurality of centrifugal slots 311 are formed in the edge of the centrifugal rotor 31, the centrifugal slots 311 are circumferentially distributed in an array along the circumferential direction of the centrifugal rotor 31, the cross section of the centrifugal slot 311 is U-shaped, the open end of the centrifugal slot 311 faces outward, and the distance between the two side walls of the centrifugal slot 311 decreases gradually from inside to outside.
Referring to fig. 3 and 4, a centrifugal cylinder 51 is disposed inside the centrifugal slot 311, a rotating shaft 511 is disposed between the centrifugal cylinder 51 and the centrifugal slot 311, an axis of the rotating shaft 511 is perpendicular to a depth direction of the centrifugal slot 311, the centrifugal cylinder 51 is rotatably connected to the centrifugal slot 311 through the rotating shaft 511, and a rotating space is reserved between an inner wall of the centrifugal slot 311 and the centrifugal cylinder 51, so that the centrifugal cylinder 51 can turn inwards or outwards around the rotating shaft 511, and when the centrifugal turntable 31 performs a centrifugal motion, the centrifugal cylinder 51 can rotate outwards around the rotating shaft 511.
With reference to fig. 3 and 4, the centrifuge tube 51 is a cylinder with an upper opening, the test tube 52 is placed inside the centrifuge tube 51, and the length of the test tube 52 is greater than that of the centrifuge tube 51, when the test tube 52 is placed inside the centrifuge tube 51, the open end of the test tube 52 is exposed outside the centrifuge tube 51, which is convenient for taking the test tube 52.
Referring to fig. 3 and 4, a pressing pad 53 is disposed between an inner wall of the centrifuge tube 51 and an outer wall of the test tube 52, the pressing pad 53 is adhered to the inner wall of the centrifuge tube 51, the pressing pad 53 is made of a silicone material, and the pressing pad 53 can fill a gap between the inner wall of the centrifuge tube 51 and the test tube 52, so that the test tube 52 is not easily separated from the centrifuge tube 51.
With reference to fig. 1 and 5, the workbench 1 is hollow, the mounting seat 6 is fixedly arranged inside the workbench 1, the mounting seat 6 is provided with a mounting cavity 61 with an upper end opening, the center of the mounting cavity 61 coincides with the center of the centrifugal turntable 31, the centrifugal turntable 31 is horizontally arranged at the opening of the mounting cavity 61, the mounting cavity 61 is cylindrical, the area of the opening end of the mounting cavity 61 is larger than the area of the centrifugal turntable 31, and a rotation space for the centrifugal drum 51 to rotate is reserved between the edge of the opening of the mounting cavity 61 and the centrifugal turntable 31.
With reference to fig. 2 and 5, the stepping motor 32 is fixedly disposed outside the mounting cavity 61, the stepping motor 32 is vertically disposed, and an output shaft of the stepping motor 32 penetrates through a circle center of the bottom wall of the mounting cavity 61 and is connected with the centrifugal shaft 33 through a key.
Combine fig. 2 and fig. 6, the inside of installation cavity 61 is provided with slurcam 7, slurcam 7 transversely strides the interval of locating between a plurality of centrifuge tubes 51, the inside of installation cavity 61 is provided with the promotion structure 8 that can order about slurcam 7 and promote the centrifuge tube 51 free end and outwards rotate, when the free end of centrifuge tube 51 outwards rotates, along with the increase of centrifuge tube 51 turned angle, the upper waste liquid that is located test tube 52 inside can be followed the open end outflow of test tube 52, the realization is to empting of upper waste liquid.
With reference to fig. 2 and 6, the pushing plate 7 includes a round rod section 71 and two square plate sections 72, wherein the round rod section 71 is disposed between the two square plate sections 72, and the round rod section 71 and the two square plate sections 72 are integrally formed.
With reference to fig. 2 and fig. 6, a plurality of pushing rods 81 are fixedly arranged on one side of the round rod section 71 of the pushing plate 7 facing the centrifugal cylinder 51, and the pushing rods 81 and the round rod section 71 are vertically arranged. The push rods 81 are arranged at equal intervals along the length direction of the push plate 7, and the positions of the push rods 81 correspond to the positions of the centrifugal cylinders 51 one by one.
Referring to fig. 6, the push rod 81 includes two clamping portions 811 and a rigid connection portion 812, the two clamping portions 811 and the rigid connection portion 812 are integrally formed, the whole push rod 81 is Y-shaped, and the centrifugal cylinder 51 can be limited between the two clamping portions 811, so that the free end of the centrifugal cylinder 51 is not easy to displace, and the stability of the centrifugal cylinder 51 is improved.
With reference to fig. 2 and fig. 6, the pushing structure 8 includes a retractable pushing member, preferably a cylinder 82, the cylinder 82 includes a cylinder 821 and a retractable rod 822, wherein the cylinder 821 is hinged to the bottom wall of the installation cavity 61, one end of the retractable rod 822 near the pushing plate 7 is integrally formed with a rotating ring 83, the rotating ring 83 is sleeved outside the round rod section 71, and the rotating ring 83 is rotatably connected to the round rod section 71 of the pushing plate 7.
With reference to fig. 2 and 6, two opposite side walls of the installation cavity 61 are respectively provided with a track groove 62, the track groove 62 is obliquely arranged, a higher end of the track groove 62 is arranged on one side close to the centrifugal cylinder 51, a lower end of the track groove 62 is arranged on one side close to the center of the installation cavity 61, the square plate section 72 is inserted into the track groove 62, and the square plate section 72 is slidably connected with the track groove 62. The number of the cylinders 82 is two, the two cylinders 82 are respectively disposed at two ends of the pushing plate 7, the two cylinders 82 are simultaneously started, the cylinders 82 can be pushed to slide along the length direction of the track groove 62, and at this time, the pushing plate 7 can push the free end of the centrifugal cylinder 51 to turn outwards.
With reference to fig. 2 and 6, as the turning angle of the centrifugal cylinder 51 increases, the upper layer waste liquid in the centrifugal cylinder 51 can flow out through the upper opening of the centrifugal cylinder 51 and flow out through the space between the opening of the centrifugal cylinder 51 and the bottom wall of the centrifugal groove 311.
With reference to fig. 3 and 5, the inside of the mounting cavity 61 is further provided with a waste liquid hopper 9 for receiving the waste liquid inside the centrifuge tube 51, the waste liquid hopper 9 is funnel-shaped, the waste liquid hopper 9 covers the outside of the centrifuge shaft 33, the axis of the waste liquid hopper 9 coincides with the axis of the centrifuge shaft 33, the orthographic projection portion of the centrifuge bowl 311 coincides with the edge of the opening end of the waste liquid hopper 9, and when the opening end of the centrifuge tube 51 rotates inward, the upper layer waste liquid in the test tube 52 can enter the waste liquid hopper 9 through the space between the centrifuge bowl 311 and the centrifuge tube 51.
With reference to fig. 3 and 5, the bottom wall of the mounting cavity 61 is provided with a liquid outlet 63 at the overlapping position of the bottom wall and the waste liquid bucket 9, the liquid outlet 63 is fixedly provided with a liquid discharge pipeline 10, one end of the liquid discharge pipeline 10 is communicated with the waste liquid bucket 9, and the other end of the liquid discharge pipeline extends to the outside of the workbench 1 and is communicated with a municipal drainage pipeline, so that the upper waste liquid is discharged.
The implementation principle of the embodiment is as follows: absorb cell turbid liquid through moving liquid mechanism 2, and release the inside to test tube 52, at the in-process that moves liquid, centrifugal carousel 31 cooperation moves liquid mechanism 2 and rotates, so that cell turbid liquid can place respectively in each test tube 52, control centrifugal shaft 33 drives centrifugal carousel 31 and rotates, make the cell in the test tube 52 with bear the weight of the solution separation, and realize adhering to the film-making, the dyeing agent drips and puts the back that finishes, control centrifugal carousel 31 rotates, make the dyeing agent fully with cell contact dyeing, the film-making and the dyeing of final completion cell.
After centrifugation is completed, two air cylinders 82 are started, the telescopic end of each air cylinder 82 pushes a push plate 7 to move along the length direction of the track groove 62, the push plate 7 drives a push rod 81 to move towards one side close to a centrifuge tube 51 until the centrifuge tube 51 is limited between two clamping parts 811 of the push rod 81, the free end of the centrifuge tube 51 is gradually turned outwards under the action of the push rod 81, along with the increase of the inclination angle of the centrifuge tube 51, upper-layer waste liquid located inside the test tube 52 can flow out through an opening of the centrifuge tube 51, the treatment of the upper-layer waste liquid in the test tube 52 is realized, a waste liquid suction tube is not required to be used for sucking the upper-layer waste liquid, the contact between reagents inside different test tubes 52 in the process of moving the upper-layer waste liquid is reduced, the influence of the waste liquid suction tube on the reagents inside the test tube 52 is further reduced, and the treatment of the upper-layer waste liquid is realized.
The embodiments of the present invention are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereby, so: all equivalent changes made according to the structure, shape and principle of the present application shall be covered by the protection scope of the present application.
Claims (9)
1. Cell film-making dyeing all-in-one with structure of falling liquid, including workstation (1) and centrifugation mechanism (3), centrifugation mechanism (3) are including centrifugation carousel (31) and drive centrifugation carousel (31) pivoted driving piece, a plurality of centrifugation grooves (311) have been seted up to the edge of centrifugation carousel (31), a plurality of centrifugation groove (311) are evenly distributed along the circumferential direction of centrifugation carousel (31), characterized by, the inside rotation of centrifugation groove (311) is provided with centrifuge bowl (51), the outside of test tube (52) is located in centrifuge bowl (51);
the centrifugal tube test bench is characterized in that the workbench (1) is arranged in a hollow manner, a mounting seat (6) is fixedly arranged in the workbench (1), a mounting cavity (61) with an opening at the upper end is formed in the mounting seat (6), a pushing plate (7) is arranged in the mounting cavity (61), the pushing plate (7) is tangent to the outer wall of the test tube (52), and the mounting seat (6) is provided with a pushing structure (8) capable of driving the pushing plate (7) to push the centrifugal tube (51) to rotate around the center of the centrifugal tube; the pushing structure (8) comprises a telescopic pushing piece, a track groove (62) is formed in the side wall of the mounting cavity (61), two ends of the pushing plate (7) are respectively inserted into the track groove (62), and the telescopic pushing piece can drive the pushing plate (7) to slide along the track groove (62).
2. A cell slide preparing and dyeing integrated machine with a liquid pouring structure as claimed in claim 1, wherein the telescopic pushing member is a cylinder (82), one end of the cylinder (82) is hinged with the bottom wall of the mounting cavity (61), and the other end is hinged with the pushing plate (7).
3. A cell slide preparing and dyeing integrated machine with a liquid pouring structure according to claim 1 is characterized in that a push rod (81) is arranged on one side of the push plate (7) facing the centrifugal cylinder (51), the push rod (81) comprises two clamping portions (811), and the two clamping portions (811) are respectively arranged on two sides of the centrifugal cylinder (51).
4. A cell sheet-making and dyeing integrated machine with a liquid pouring structure as claimed in claim 3, wherein the number of the push rods (81) is multiple, and the multiple push rods (81) are arranged at equal intervals along the length direction of the push plate (7).
5. A cell slide-making and dyeing integrated machine with a liquid pouring structure according to claim 1, characterized in that a waste liquid hopper (9) for receiving waste liquid in a centrifugal cylinder (51) is arranged in the installation cavity (61), and the orthographic projection part of the centrifugal groove (311) coincides with the upper end opening of the waste liquid hopper (9).
6. A cell sheet-making and dyeing integrated machine with a liquid pouring structure according to claim 5, characterized in that a liquid outlet (63) is opened on the bottom wall of the waste liquid bucket (9), a liquid discharge pipeline (10) is communicated with the liquid outlet (63), and the liquid discharge pipeline (10) extends to the outside of the workbench (1).
7. A cell sheet-making and dyeing integrated machine with a liquid pouring structure as claimed in claim 1, wherein the driving member is a stepping motor (32), and the output end of the stepping motor (32) is fixedly connected with the centrifugal turntable (31).
8. A cell sheet-making and dyeing integrated machine with a liquid pouring structure according to claim 1, characterized in that a squeezing pad (53) is arranged between the inner wall of the centrifugal cylinder (51) and the outer wall of the test tube (52), and the squeezing pad (53) is fixedly connected with the centrifugal cylinder (51).
9. A cell sheet-making and dyeing integrated machine with a liquid pouring structure according to claim 8, characterized in that the centrifugal cylinder (51) is a cylinder body with an upper end opening, and the length of the test tube (52) is greater than that of the centrifugal cylinder (51).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110579662.9A CN113188871B (en) | 2021-05-26 | 2021-05-26 | Cell film-making dyeing all-in-one with liquid structure falls |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110579662.9A CN113188871B (en) | 2021-05-26 | 2021-05-26 | Cell film-making dyeing all-in-one with liquid structure falls |
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| Publication Number | Publication Date |
|---|---|
| CN113188871A CN113188871A (en) | 2021-07-30 |
| CN113188871B true CN113188871B (en) | 2022-12-20 |
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| CN202110579662.9A Active CN113188871B (en) | 2021-05-26 | 2021-05-26 | Cell film-making dyeing all-in-one with liquid structure falls |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2388563B (en) * | 2002-05-17 | 2004-05-19 | Hitachi Koki Kk | Bio cell cleaning centrifuge having bio cell cleaning rotor provided with cleaning liquid distributor |
| JP4110454B2 (en) * | 2002-05-17 | 2008-07-02 | 日立工機株式会社 | Cell washing centrifuge |
| IL215257A (en) * | 2011-09-20 | 2016-07-31 | Amir Zilberberg | Apparatus and method for transferring test tubes |
| CN207170028U (en) * | 2017-08-28 | 2018-04-03 | 魏莉 | A kind of manual centrifugal device from main regulation test tube angle of inclination |
| CN108168986B (en) * | 2018-03-21 | 2024-08-30 | 深圳市鹏一医疗仪器有限公司 | Full-automatic liquid-based cell slide-making and dyeing integrated machine and slide-making and dyeing method |
| CN109622246A (en) * | 2019-01-28 | 2019-04-16 | 张家港市新华化工机械有限公司 | Centrifuge is used in a kind of experiment |
| CN211946981U (en) * | 2020-03-04 | 2020-11-17 | 郑州奥博细胞医学实验室有限公司 | Stem cell humidifying centrifugal equipment |
| CN213022545U (en) * | 2020-09-14 | 2021-04-20 | 南京健邦锦源医疗仪器有限公司 | Cell separation film-making dyeing all-in-one |
| CN112779137A (en) * | 2021-01-27 | 2021-05-11 | 王墨权 | Stem cell culture method |
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2021
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