CN214881445U

CN214881445U - Cord blood stem cell separation detection device

Info

Publication number: CN214881445U
Application number: CN202120828886.4U
Authority: CN
Inventors: 梁荣博
Original assignee: Jiangxi Jinchang Stem Cell Genetic Engineering Co ltd
Current assignee: Jiangxi Jinchang Stem Cell Genetic Engineering Co ltd
Priority date: 2021-04-22
Filing date: 2021-04-22
Publication date: 2021-11-26
Anticipated expiration: 2031-04-22

Abstract

The utility model relates to a cell separation detects technical field, specifically is a cord blood stem cell separation detection device, including the base, preprocessor has been put at the base top, and preprocessor includes dilution tank, connecting pipe and gunbarrel, and the dilution tank top is equipped with blood feed liquor pipe, and the gunbarrel lateral wall is equipped with diluent feed liquor pipe, and the gunbarrel lateral wall is equipped with precipitant feed liquor pipe, and the gunbarrel bottom is equipped with the fluid-discharge tube and arranges the waste pipe, and base one side is equipped with the base, and the base top is rotated even and is rotated the seat, has put the centrifuging tube on rotating the seat. This cord blood stem cell separation detection device, umbilical cord blood are through diluting in diluting the jar, get into the gunbarrel after diluting, inject the erythrocyte precipitant into the gunbarrel, deposit the erythrocyte, and the turbid liquid of lower floor's erythrocyte discharges through the exhaust tube, and upper cell sap gets into the centrifuging tube through the fluid-discharge tube in, drives the centrifuging tube through rotating the seat and rotates, with cell sap centrifugation layering, through flowing back layer upon layer, reduces the solution pollution, improves and detects the accuracy.

Description

Cord blood stem cell separation detection device

Technical Field

The utility model relates to a cell separation detects technical field, specifically is a cord blood stem cell separation detection device.

Background

The cord blood is blood in the umbilical cord and in the blood vessel on the side close to the fetus of the placenta when the fetus is born, contains a large amount of stem cells, has weak lymphocyte antigenicity in the cord blood, relatively immature functions and low risk of acute graft-versus-host disease after transplantation, is an important way for stem cell transplantation, has wide application in the treatment of malignant tumor, aplastic anemia, partial hereditary diseases and the like in a blood system, and has the advantages that the cord blood stem cells need to be separated and detected before being put into use, are comprehensively evaluated and improve the use safety performance.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a cord blood stem cell separation detection device to solve the problem that provides among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme:

a cord blood stem cell separation detection device, includes the base, preprocessor has been placed at the base top, preprocessor includes the dilution jar, dilution jar bottom intermediate position is equipped with the connecting pipe rather than inside and link up mutually, install first control valve on the connecting pipe, just the connecting pipe bottom is equipped with the gunbarrel rather than inside and link up mutually, dilution jar top is equipped with the blood feed liquor pipe rather than inside and link up mutually, dilution jar lateral wall is close to its top edge and is equipped with the diluent feed liquor pipe rather than inside and link up mutually, diluent feed liquor pipe upper end is along vertical direction and its top height is higher than dilution jar top height, dilution jar lateral wall is close to its bottom edge and is equipped with the intake pipe rather than inside and link up mutually, intake pipe upper end is along vertical direction and its top height is higher than dilution jar top height, the top edge of the outer side wall of the dilution tank is provided with a plurality of exhaust holes which are uniformly distributed and communicated with the inside of the dilution tank, the outer side wall of the precipitation tank is provided with a precipitant liquid inlet pipe communicated with the inside of the precipitation tank near the top edge of the outer side wall of the precipitation tank, the upper end of the precipitant liquid inlet pipe is in the vertical direction, the top of the precipitant liquid inlet pipe is higher than the top of the precipitation tank, the bottom of the precipitation tank is provided with a liquid discharge pipe and a waste discharge pipe which are inclined downwards and communicated with the inside of the precipitation tank, the liquid discharge pipe is provided with a second control valve, the waste discharge pipe is provided with a third control valve, one side of the base is provided with a base, the top of the base is rotatably connected with a rotating seat, the bottom of the base is provided with a mounting groove, the top of the mounting groove is provided with a miniature speed reducing motor, an output shaft of the miniature speed reducing motor vertically upwards passes through the base, the tail end of the miniature speed reducing motor is connected to the rotating seat, the top of the rotating seat is provided with a placing groove, a centrifugal tube with an open top is placed in the placing groove and is positioned right below the tail end of the liquid discharge tube.

Preferably, dilution tank and sedimentation tank bottom all are hopper-shaped structure, the sedimentation tank bottom is close to its edge all around and is equipped with a plurality of supporting legss that are the annular array and distribute, the supporting legs is placed on the base.

Preferably, the dilution tank, the connecting pipe, the sedimentation tank and the supporting legs are all in an integrally formed structure.

Preferably, the top of the blood liquid inlet pipe, the diluent liquid inlet pipe and the precipitant liquid inlet pipe is provided with a liquid inlet funnel which is of an integrated structure.

Preferably, the top of the base is provided with a groove, a ball bearing is installed in the groove, the bottom of the rotating base is coaxially connected with an adapter rod which is of an integrally formed structure, and the adapter rod is fixed in an inner ring of the ball bearing.

Preferably, the lower end of the outer side wall of the base is provided with a plurality of radiating grooves which are uniformly distributed and are communicated with the mounting groove.

Preferably, a rubber pad is embedded on the inner wall of the placing groove.

Compared with the prior art, the beneficial effects of the utility model are that:

this cord blood stem cell separation detection device, when using, in the cord blood gets into the dilution jar through blood feed liquor pipe, pour the diluent into the dilution jar through diluent feed liquor pipe, dilute the cord blood, the cord blood solution after diluting gets into the precipitation jar in, pour into the erythrocyte precipitant through the precipitant feed liquor pipe into the precipitation jar, deposit the erythrocyte, discharge lower floor's erythrocyte turbid liquid through the exhaust pipe again, upper cell liquid gets into the centrifuging tube through the fluid-discharge tube in, output shaft through miniature gear motor rotates, drive and rotate seat and centrifuging tube and rotate, with the cell liquid centrifugation layering, pour out after the supernatant, lower floor's stem cell solution collects in the centrifuging tube, detect it again can, through flowing back layer upon layer, avoid liquid extraction, reduce the risk of solution pollution, improve the accuracy of testing result.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic structural diagram of a preprocessor according to the present invention;

FIG. 3 is a schematic structural view of the base of the present invention;

fig. 4 is a schematic structural view of the rotary seat of the present invention.

In the figure:

1. a base;

2. a preprocessor; 20. a dilution tank; 200. an exhaust hole; 21. a connecting pipe; 210. a first control valve; 22. a settling tank; 23. supporting legs;

3. a blood inlet pipe;

4. a diluent inlet pipe;

5. an air inlet pipe;

6. a precipitant liquid inlet pipe;

7. a liquid discharge pipe; 70. a second control valve;

8. a waste discharge pipe; 80. a third control valve;

9. a base; 90. a ball bearing; 91. a heat sink;

10. a rotating seat; 100. a placement groove; 101. a transfer lever;

11. a miniature speed reduction motor;

12. and (6) centrifuging the tube.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.

Furthermore, the terms "first", "second", "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, features defined as "first", "second", "third" may explicitly or implicitly include one or more of the features. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.

Referring to fig. 1-4, the present invention provides a technical solution:

a cord blood stem cell separation detection device comprises a base 1, a preprocessor 2 is placed at the top of the base 1, the preprocessor 2 comprises a dilution tank 20, a connecting pipe 21 communicated with the interior of the dilution tank 20 is arranged at the middle position of the bottom of the dilution tank 21, a first control valve 210 is installed on the connecting pipe 21, a settling tank 22 communicated with the interior of the connecting pipe 21 is arranged at the bottom of the connecting pipe 21, a plurality of supporting legs 23 distributed in an annular array are arranged at the bottom of the settling tank 22 close to the peripheral edge of the bottom of the settling tank, the supporting legs 23 are placed on the base 1, a blood liquid inlet pipe 3 communicated with the interior of the dilution tank 20 is arranged at the top of the dilution tank 20, a diluent liquid inlet pipe 4 communicated with the interior of the dilution tank is arranged at the outer side wall of the dilution tank 20 close to the top edge of the dilution tank, a precipitant liquid inlet pipe 6 communicated with the interior of the settling tank 22 is arranged at the outer side wall of the settling tank close to the top edge of the outer side wall of the settling tank, a liquid outlet pipe 7 and a waste discharge pipe 8 communicated with the interior of the settling tank are arranged obliquely downwards, the second control valve 70 is arranged on the liquid discharge pipe 7, the third control valve 80 is arranged on the waste discharge pipe 8, the umbilical cord blood enters the diluting tank 20 through the blood liquid inlet pipe 3, the diluent is injected into the diluting tank 20 through the diluent liquid inlet pipe 4 to dilute the umbilical cord blood, the diluted umbilical cord blood solution enters the settling tank 22 through the connecting pipe 21, the erythrocyte precipitator is injected into the settling tank 22 through the precipitator liquid inlet pipe 6 to precipitate the erythrocytes, then the lower layer erythrocyte turbid liquid is discharged through the waste discharge pipe 8, and the upper layer cell liquid is discharged through the liquid discharge pipe 7.

In this embodiment, a base 9 is disposed on one side of a base 1, a rotating seat 10 is rotatably connected to the top of the base 9, a mounting groove is disposed at the bottom of the base 9, a micro gear motor 11 is mounted at the top of the mounting groove, an output shaft of the micro gear motor 11 vertically passes upward through the base 9, and the end of the output shaft is connected to the rotating seat 10, a placing groove 100 is disposed at the top of the rotating seat 10, a centrifuge tube 12 with an open top is disposed in the placing groove 100, the centrifuge tube 12 is located under the end of a liquid discharge tube 7, upper-layer cell sap discharged from the liquid discharge tube 7 flows into the centrifuge tube 12, the rotation of the output shaft of the micro gear motor 11 drives the rotating seat 10 and the centrifuge tube 12 to rotate, the cell sap is centrifugally layered, after upper-layer clear liquid is poured out, lower-layer dry cell sap is collected in the centrifuge tube 12 and then detected, liquid is discharged layer by layer, liquid extraction is avoided, and the risk of solution pollution is reduced, the accuracy of the detection result is improved; inlay on the 100 inner walls of standing groove and have the rubber pad, increase the frictional force between centrifuging tube 12 and the 100 inner walls of standing groove through the rubber pad, prevent to rotate seat 10 and drive centrifuging tube 12 and rotate when centrifugation, take place relative rotation between centrifuging tube 12 and the rotation seat 10, influence centrifugal effect.

In addition, the outer side wall of the dilution tank 20 is provided with an air inlet pipe 5 communicated with the inside of the dilution tank near the bottom edge of the outer side wall of the dilution tank 20, the top edge of the outer side wall of the dilution tank 20 is provided with a plurality of exhaust holes 200 which are uniformly distributed and communicated with the inside of the dilution tank, air is blown into the dilution tank 20 through the air inlet pipe 5 to stir the gas easily, the dilution of the umbilical cord blood is accelerated, the blown air is discharged from the exhaust holes 200, and the stable air pressure in the dilution tank 20 is ensured; the upper end of the air inlet pipe 5 is in the vertical direction, and the top height of the air inlet pipe is higher than that of the dilution tank 20, so that the dilution tank 20 is prevented from easily overflowing from the air inlet pipe 5.

Further, the upper end of the diluent liquid inlet pipe 4 is vertical and the top height of the diluent liquid inlet pipe is higher than the top height of the dilution tank 20, so that the diluent in the dilution tank 20 is prevented from easily overflowing from the diluent liquid inlet pipe 4; the upper end of the precipitator liquid inlet pipe 6 is vertical and the top height of the precipitator liquid inlet pipe is higher than the top height of the precipitation tank 22, so that the solution in the precipitation tank 22 is prevented from overflowing from the precipitator liquid inlet pipe 6.

Secondly, the bottoms of the dilution tank 20 and the sedimentation tank 22 are funnel-shaped structures, so that liquid can be discharged conveniently; blood feed liquor pipe 3, diluent feed liquor pipe 4 and 6 tops of precipitant feed liquor pipe all are equipped with rather than the feed liquor funnel that is the integrated into one piece structure, increase the size of blood feed liquor pipe 3, diluent feed liquor pipe 4 and 6 top port departments of precipitant feed liquor pipe through the feed liquor funnel, make things convenient for the feed liquor.

Furthermore, a groove is formed in the top of the base 9, a ball bearing 90 is installed in the groove, an adapter rod 101 which is integrally formed with the bottom of the rotating seat 10 is coaxially connected to the bottom of the rotating seat 10, the adapter rod 101 is fixed in an inner ring of the ball bearing 90, and the sensitivity of the rotation of the rotating seat 10 driven by an output shaft of the miniature speed reduction motor 11 is ensured through the ball bearing 90, so that the clamping is prevented; a plurality of radiating grooves 91 which are evenly distributed and are communicated with the mounting groove are formed in the lower end of the outer side wall of the base 9, the radiating grooves 91 are used for accelerating the heat inside the mounting groove to be radiated, and the normal use of the miniature speed reduction motor 11 is prevented from being influenced by excessive heat collected inside the mounting groove.

Specifically, dilution tank 20, connecting pipe 21, gunbarrel 22 and supporting legs 23 all are the integrated into one piece structure, and the integrated into one piece structure is convenient for produce, and the product size precision of producing is high, saves raw materials, and the integrated into one piece stable in structure, non-deformable damages in the use, long service life.

When the cord blood stem cell separation and detection device is used, a worker firstly checks the integrity of the device and whether the connection of all parts is firm; then first control valve 210, second control valve 70, and third control valve 80 are closed; then, the umbilical cord blood enters the diluting tank 20 through the blood liquid inlet pipe 3, then the diluent is injected into the diluting tank 20 through the diluent liquid inlet pipe 4, the umbilical cord blood is diluted, meanwhile, gas is blown into the diluting tank 20 through the gas inlet pipe 5, the gas is easily stirred, the dilution of the umbilical cord blood is accelerated, the blown air is discharged from the exhaust hole 200, and the stable air pressure in the diluting tank 20 is ensured; after the dilution is finished, the first control valve 210 is opened, the diluted umbilical cord blood solution enters the sedimentation tank 22 through the connecting pipe 21, and then the erythrocyte precipitator is injected into the sedimentation tank 22 through the precipitator liquid inlet pipe 6 to precipitate the erythrocytes; after the precipitation is finished, opening the third control valve 80, discharging and collecting the lower layer of erythrocyte turbid liquid through the waste discharge pipe 8, immediately closing the third control valve 80 after the lower layer of erythrocyte turbid liquid is discharged, then opening the second control valve 70, discharging the upper layer of cell liquid through the liquid discharge pipe 7, and allowing the upper layer of cell liquid to flow into the centrifuge tube 12; then, a power supply of the miniature speed reducing motor 11 is connected to enable the miniature speed reducing motor to work, and an output shaft of the miniature speed reducing motor 11 rotates to drive the rotating seat 10 and the centrifugal tube 12 to rotate so as to centrifugally stratify the cell sap; after the centrifugation is completed, the power supply of the miniature speed reduction motor 11 is disconnected, the centrifuge tube 12 is taken out after the rotation seat 10 and the centrifuge tube 12 stop rotating, the supernatant in the centrifuge tube 12 is poured out, and the stem cell solution at the lower layer is collected in the centrifuge tube 12.

The foregoing shows and describes the general principles, essential features, and advantages of the invention. It should be understood by those skilled in the art that the present invention is not limited by the above embodiments, and the description in the above embodiments and the description is only preferred examples of the present invention, and is not intended to limit the present invention, and that the present invention can have various changes and modifications without departing from the spirit and scope of the present invention, and these changes and modifications all fall into the scope of the claimed invention. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. The utility model provides a cord blood stem cell separation detection device which characterized in that: including base (1), pretreater (2) has been placed at base (1) top, pretreater (2) are including diluting jar (20), diluting jar (20) bottom intermediate position is equipped with connecting pipe (21) rather than inside and having a perfect understanding mutually, install first control valve (210) on connecting pipe (21), just connecting pipe (21) bottom is equipped with sedimentation tank (22) rather than inside and having a perfect understanding mutually, diluting jar (20) top is equipped with rather than inside blood feed liquor pipe (3) that have a perfect understanding mutually, diluting jar (20) lateral wall is close to its top edge and is equipped with the diluent feed liquor pipe (4) rather than inside and having a perfect understanding mutually, diluent feed liquor pipe (4) upper end is along vertical direction and its top height is higher than diluting jar (20) top height, diluting jar (20) lateral wall is close to its bottom edge and is equipped with rather than inside intake pipe (5) that have a perfect understanding mutually, the upper end of the air inlet pipe (5) is vertically arranged and the top of the air inlet pipe is higher than the top of the dilution tank (20), a plurality of exhaust holes (200) which are uniformly distributed and communicated with the inside of the dilution tank are formed in the top edge of the outer side wall of the dilution tank (20), a precipitator liquid inlet pipe (6) which is communicated with the inside of the precipitation tank is arranged on the outer side wall of the precipitation tank (22) close to the top edge of the outer side wall of the precipitation tank, the upper end of the precipitator liquid inlet pipe (6) is vertically arranged and the top of the precipitator liquid inlet pipe is higher than the top of the precipitation tank (22), a liquid discharge pipe (7) and a waste discharge pipe (8) which are obliquely downward and communicated with the inside of the precipitation tank are arranged at the bottom of the precipitation tank (22), a second control valve (70) is arranged on the liquid discharge pipe (7), a third control valve (80) is arranged on the waste discharge pipe (8), a base (9) is arranged on one side of the base (1), and the top of the base (9) is rotatably connected with a rotating seat (10), the mounting groove has been seted up to base (9) bottom, and miniature gear motor (11) are installed at the mounting groove top, the output shaft of miniature gear motor (11) is vertical upwards to pass base (9) and its end-to-end connection on rotating seat (10), rotating seat (10) top and having seted up standing groove (100), open-top's centrifuging tube (12) have been placed in standing groove (100), centrifuging tube (12) are located under fluid-discharge tube (7) end.

2. The cord blood stem cell separation and detection device according to claim 1, characterized in that: dilution tank (20) and settling cask (22) bottom all are hopper-shaped structure, settling cask (22) bottom is close to its edge all around and is equipped with a plurality of supporting legss (23) that are the ring array and distribute, supporting legss (23) are placed on base (1).

3. The cord blood stem cell separation and detection device according to claim 2, characterized in that: the dilution tank (20), the connecting pipe (21), the settling tank (22) and the supporting legs (23) are all of an integrally formed structure.

4. The cord blood stem cell separation and detection device according to claim 1, characterized in that: blood feed liquor pipe (3) diluent feed liquor pipe (4) with precipitant feed liquor pipe (6) top all is equipped with rather than the feed liquor funnel that is integrated into one piece structure.

5. The cord blood stem cell separation and detection device according to claim 1, characterized in that: the novel rotary table is characterized in that a groove is formed in the top of the base (9), a ball bearing (90) is installed in the groove, an adapter rod (101) which is of an integrally formed structure is coaxially connected to the bottom of the rotary base (10), and the adapter rod (101) is fixed in an inner ring of the ball bearing (90).

6. The cord blood stem cell separation and detection device according to claim 1, characterized in that: the lower end of the outer side wall of the base (9) is provided with a plurality of radiating grooves (91) which are uniformly distributed and are communicated with the mounting grooves.

7. The cord blood stem cell separation and detection device according to claim 1, characterized in that: rubber pads are embedded on the inner walls of the placing grooves (100).