CN221071439U - Cell centrifugation container, cell separation device and cell separation system - Google Patents

Abstract

The utility model discloses a cell centrifugation container, which comprises a body, a separation cavity arranged in the body, a first pipeline extending to the bottom of the separation cavity, a contraction part arranged in the separation cavity, a conical contraction cavity formed in the contraction part, a contraction opening communicated with the bottom of the separation cavity and arranged at the conical tip part of the contraction cavity, and a second pipeline extending to the contraction opening. A cell separation device and a cell separation system are also disclosed. The cell centrifugation container, the cell separation device, the cell separation system and the cell separation method have the advantages of improving the target cell quantity, collecting the cell yield and the like.

Description

Cell centrifugation container, cell separation device and cell separation system

Technical Field

The utility model relates to the technical field of food and medicine packaging mechanical equipment, in particular to a cell centrifugation container, a cell separation device and a cell separation system.

Background

PBMC is peripheral blood mononuclear cells, human peripheral blood contains lymphocytes, monocytes, dendritic cells and other small amount of cells, a large amount of immune cells can be obtained for cell therapy through separation, screening and culture of PBMC, and the existing PBMC is separated manually by scientific researchers through a centrifuge tube and a centrifuge under a clean environment, so that high requirements are provided for the technical level of operators, the efficiency is low, the open type operation brings great pollution risk, and meanwhile, the product quality is uneven due to the influence of human factors.

The present cell centrifugation container, for example, "a cell centrifugation container, a cell separation apparatus and a cell separation system" with application number CN202310068199.0, includes a first tube extending to the bottom of the container and a second tube in the middle of the container, where the second tube is used for discharging the separated supernatant, since the second tube is located in the middle of the container, the liquid level area of the supernatant located in the second tube is large, the liquid outlet of the second tube only occupies a small part of the liquid level of the supernatant, in the liquid discharging process, only the liquid near the liquid outlet can be discharged, the supernatant far away from the liquid outlet cannot be discharged, and in the liquid discharging process, the target cell layer located in the lower layer of the supernatant can be discharged, resulting in low cell yield.

In addition, in the centrifugal separation process of the cell centrifugation container, target cells and reagents are both in the collection cavity, and the contact rate of the relative volume of the contact surface of the target cells and the side wall of the collection cavity is high, so that the yield of the target cells is affected; and when collecting the target cell, the target cell is located in the collecting cavity and is collected through the pipeline extending into the bottom of the collecting cavity, the capacity of the collecting cavity is small, the target cell quantity is small, the yield is low, or the separating cavity above the collecting cavity is collected through the pipeline extending into the separating cavity, at this time, the area of the surface of the separating cavity corresponding to the bottom surface of the pipeline extending into the separating cavity is large, the target cell is difficult to collect completely, and the yield of the cell is reduced.

Disclosure of utility model

The utility model aims to solve the technical problem of overcoming the defects of the prior art and providing a cell centrifugation container, a cell separation device and a cell separation system which can improve the target cell quantity and the yield of collected cells.

In order to solve the technical problems, the utility model adopts the following technical scheme:

The utility model provides a cell centrifugation container, includes the body, locates this internal separation chamber and extends to the first pipeline of separation chamber bottom, be equipped with the constriction in the separation chamber, be formed with the conical constriction chamber in the constriction, the conical tip portion in constriction chamber towards the bottom of separation chamber to be equipped with the shrink mouth with separation chamber bottom intercommunication, cell centrifugation container is still including extending to the second pipeline of shrink mouth.

As a further improvement of the above technical scheme:

The end face of the shrinkage part facing the bottom of the separation cavity is a conical surface with a conical tip part connected with the shrinkage opening.

The outer side wall of the shrinkage part is matched and sleeved with the inner wall of the separation cavity.

The bottom of separation chamber is equipped with the collection chamber, first pipeline extends to the collection chamber.

The separation cavity comprises a conical portion, and the bottom of the separation cavity is located at the conical tip portion of the conical portion.

The body comprises a centrifugal cup and a cup cover, the separation cavity is formed in the centrifugal cup, and the cup cover is provided with an opening of the centrifugal cup.

The cup cover is provided with a ventilated membrane, the first pipeline penetrates through the middle of the cup cover, and the second pipeline is coaxially sleeved with the first pipeline.

The cup cover is provided with a first connector and a second connector, the first pipeline is connected with the first connector, and the second pipeline is connected with the second connector.

The cell separation device comprises a rotating frame and the cell centrifugation container, wherein the body is rotatably arranged on the rotating frame, a swinging sleeve capable of vertically rotating is arranged on the rotating frame, and the body is sleeved in the swinging sleeve.

The utility model provides a cell separation system, includes open-top's separation chamber, be equipped with foretell cell separation device in the separation chamber, be equipped with the rotatory actuating mechanism of swivel mount rotatory that is used for driving cell separation device on the separation chamber, the opening part of separation chamber is equipped with the apron, be equipped with control module on the separation chamber and be used for hanging the rack of putting the liquid supply device, liquid supply device and rotatory actuating mechanism all are connected with control module, the separation chamber passes through pipeline and is connected with liquid supply device.

A cell separation method is carried out by adopting the cell centrifugation container and comprises the following steps:

S1, adding target cells and a separating solution: adding cell liquid with target cells and separating liquid into the separating cavity through a first pipeline, so that the separating liquid is below the cell liquid;

S2, centrifugal delamination: the body centrifugally moves, the cell fluid separates out target cells under the action of centrifugal force, the target cells are positioned in the contraction cavity, and the separation fluid is arranged between the target cells and the conical tip part of the separation cavity;

S3, taking out target cells: and taking out the target cells in the contraction cavity through a second pipeline.

Compared with the prior art, the utility model has the advantages that:

The cell centrifugation container of the utility model comprises the following centrifugal separation processes: firstly, adding cell liquid with cells of a mesh and separating liquid into a separating cavity through a first pipeline, so that the separating liquid is positioned below the cell liquid, and separating the separating liquid into layers below the cell liquid; secondly, the body centrifugally moves, the cell fluid separates out target cells under the action of centrifugal force, the target cells are positioned in the shrinkage cavity, and the separation fluid is arranged between the target cells and the cone tip part of the separation cavity; finally, the target cells in the shrink cavity are taken out through the second pipeline. Thus, the separated target cells are positioned in the contraction cavity, the contact rate of the relative volume of the contact surface of the separated target cells and the side wall of the contraction cavity is small, and the yield of the target cells is high; and when collecting the target cells, the second pipeline extending to the contraction opening is used, the area of the surface of the contraction cavity corresponding to the bottom surface of the second pipeline is close to or equal to the area of the contraction opening, the area is small, the target cells can be collected more fully, and the yield of the cells is improved.

The cell separation device of the utility model comprises all technical features of the cell centrifugation container and has all advantages of the cell centrifugation container.

The cell separation system of the present utility model includes all technical features of the cell separation apparatus, and has all advantages of the cell separation apparatus.

Drawings

FIG. 1 is a schematic perspective view of a centrifuge vessel according to the present utility model.

FIG. 2 is a schematic cross-sectional view of a centrifuge vessel according to the present utility model.

Fig. 3 is an enlarged view at a in fig. 2.

Fig. 4 is an enlarged view at B in fig. 2.

FIG. 5 is a schematic perspective view of a cell separation apparatus according to the present utility model.

FIG. 6 is a schematic perspective view of a cell separation system according to the present utility model.

FIG. 7 is an open state diagram of the cell separation system of the present utility model.

Fig. 8 is an enlarged view at C in fig. 7.

FIG. 9 is a schematic diagram showing addition of a cell liquid and a separation liquid in the cell separation method of the present utility model.

FIG. 10 is a schematic diagram of a centrifugation delamination method of the cell separation method of the present utility model.

FIG. 11 is a schematic representation of the cell separation method of the present utility model after erythrocyte aspiration.

FIG. 12 is a schematic diagram of the cell separation method of the present utility model after the objective cells are aspirated.

FIG. 13 is a schematic diagram showing another embodiment of the cell separation method of the present utility model, wherein FIG. a is a schematic diagram showing the addition of a cell sap; b, adding a protective liquid to form a layered schematic diagram; c, a schematic diagram after centrifugal layering; and d is a schematic diagram after the supernatant is discharged.

The reference numerals in the drawings denote:

1. A body; 11. a centrifugal cup; 12. a cup cover; 121. a breathable film; 122. a first joint; 123. a second joint; 2. a separation chamber; 21. a collection chamber; 3. a first pipe; 4. a constriction; 41. a shrink chamber; 42. a shrinking opening; 5. a second pipe; 6. a rotating frame; 7. a swinging sleeve; 8. a separation chamber; 81. a cover plate; 82. a control module; 83. a hanging rack; 9. cell fluid; 91. a cell of interest; 92. separating liquid.

Detailed Description

The utility model will be described in further detail with reference to the drawings and the specific examples.

In the description of the present application, it should be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate orientations or positional relationships based on the orientation or positional relationships shown in the drawings, merely to facilitate describing the present application and simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present application.

Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present application, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.

In the present application, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art according to the specific circumstances.

Embodiment one:

Fig. 1 to 4 show an embodiment of the cell centrifugation container according to the present utility model, the cell centrifugation container according to the present embodiment comprises a body 1, a separation chamber 2 provided in the body 1, and a first pipe 3 extending to the bottom of the separation chamber 2, a constriction 4 provided in the separation chamber 2, a constriction chamber 41 formed in a tapered shape in the constriction 4, a tapered tip of the constriction chamber 41 facing the bottom of the separation chamber 2 and provided with a constriction 42 communicating with the bottom of the separation chamber 2, and a second pipe 5 extending to the constriction 42.

The centrifugal separation process of the cell centrifugal container comprises the following steps: firstly, a cell solution 9 with a target cell 91 and a separation solution 92 are added into a separation chamber 2 through a first pipeline 3, and the separation solution 92 is arranged below the cell solution 9, so that the separation solution 92 is separated into layers below the cell solution; secondly, the body 1 centrifugally moves, the cell liquid 9 separates out target cells 91 under the action of centrifugal force, the target cells 91 are positioned in the contraction cavity 41, and the separation liquid 92 is arranged between the target cells 91 and the cone tip part of the separation cavity 2; finally, the target cells in the constriction 41 are removed through the second conduit 5. Thus, the separated target cells 91 are positioned in the contraction cavity 41, the contact rate of the relative volume of the contact surface with the side wall of the contraction cavity 41 is small, and the yield of the target cells is high; when collecting the target cells, the second pipe 5 extending to the constriction opening 42 is used, and the area of the surface of the constriction chamber 41 corresponding to the bottom surface of the second pipe 5 is close to or equal to the area of the constriction opening 42, so that the target cells can be collected more sufficiently with a small area, and the yield of the cells can be improved.

Further, as shown in fig. 2, in this embodiment, the end surface of the constriction 4 facing the bottom of the separation chamber 2 is a conical surface with a conical tip connected to the constriction 42. Thus, after the cell liquid 9 with the cell 91 of the mesh is added through the first pipeline 3 extending to the bottom of the separation cavity 2, the cell liquid 9 enters the bottom of the separation cavity 2 first, then enters the contraction part 4 through the contraction opening 42, the end surface of the contraction part 4 facing the bottom of the separation cavity 2 is a conical surface with a conical tip part connected with the contraction opening 42, so that the cell liquid 9 is conveniently guided to the contraction opening 42 and enters the contraction cavity 41, and the liquid in the contraction cavity 41 is also conveniently guided to the bottom of the separation cavity 2.

Further, in this embodiment, the outer side wall of the constriction 4 is fit and sleeved with the inner wall of the separation chamber 2. Is convenient for assembly and processing.

Further, in the present embodiment, the bottom of the separation chamber 2 is provided with a collecting chamber 21, and the first pipe 3 extends to the collecting chamber 21. Facilitating the discharge of the corresponding liquid, such as the separation liquid 92, through the first conduit 3. The collecting cavity 21 is a conical space, and is beneficial to the sedimentation of relevant liquid to the cone bottom under the action of centrifugal force.

Further, in the present embodiment, the separation chamber 2 includes a tapered portion, and the bottom of the separation chamber 2 is located at the tapered tip of the tapered portion. The contraction part 4 is positioned in the conical part of the separation cavity 2 and is matched and sleeved with the inner wall of the conical part of the separation cavity 2. The centre line of the constriction 4 is collinear with the conical portion of the separation chamber 2.

Further, in this embodiment, the body 1 includes a centrifugal cup 11 and a cup cover 12, the separation chamber 2 is formed in the centrifugal cup 11, and the cup cover 12 covers an opening of the centrifugal cup 11.

Further, in this embodiment, the air-permeable membrane 121 is disposed on the cup cover 12, the first pipe 3 is disposed through the middle of the cup cover 12, and the second pipe 5 is sleeved with the first pipe 3 coaxially. Preferably, the first duct 3 is provided through the second duct 5. A sealing gasket is arranged between the centrifugal cup 11 and the cup cover 12.

Further, in this embodiment, the cup cover 12 is provided with a first connector 122 and a second connector 123, the first pipe 3 is connected to the first connector 122, and the second pipe 5 is connected to the second connector 123. A connector is provided to facilitate connection to an external container or device. Ensuring that the body 1 can load or discharge liquid with an external pipeline in a rotating state.

As shown in fig. 9 to 12, the present cell centrifugation container has a specific application scheme of:

1) In a static state, the cup body 1 is positioned with the cone tip facing downwards. Blood is added to the cup 1 through the first connector 122, the first tubing 3, as shown in fig. 9;

2) Separating liquid 92 is slowly added to the conical bottom of the cup body 1 through the first joint 122 and the first pipeline 3, and the separating liquid 92 and the upper blood form a layer, as shown in fig. 9;

3) The cup body 1 is in a cone tip outward state, red blood cells in blood sink to the cone bottom of the cup body 1 under the action of centrifugal force, target cells 91 in blood sink to the upper surface of the separating liquid 92 under the action of centrifugal force, and red blood cells, the separating liquid 92, the target cells 91 and supernatant are sequentially arranged from the cup cone bottom to the cup cover 12, as shown in fig. 10;

4) After the centrifugal speed is reduced to a given speed, the red blood cells at the bottom of the cup and part of the separating liquid 92 are discharged through the first joint 122 and the first pipeline 3, so that the target cells 91 are ensured to be positioned above the contraction opening 42, as shown in fig. 11;

5) Collecting a small amount of separating liquid 92, target cells 91 and a small amount of supernatant above the constriction 42 through the second joint 123 and the second pipeline 5, and after the collection, collecting residual separating liquid 92 at the bottom of the cup 1 and residual supernatant above the constriction 42, as shown in fig. 12;

6) Residual liquid in the cup 1 is discharged through the first joint 122 and the first pipe 3.

As shown in fig. 13, the specific application scheme of the present cell centrifugation container is two:

1) In a static state, the cup body 1 is positioned with the cone tip facing downwards. Adding cell sap to the cup 1 through the first joint 122 and the first pipe 3 as shown in a diagram of fig. 13;

2) Slowly adding a protective liquid (separating liquid 92) to the conical bottom of the cup body 1 through the first joint 122 and the first pipeline 3, wherein the protective liquid and the cell liquid above form layering, as shown in a diagram b in fig. 13;

3) The cup body 1 is in a state that the cone tip faces outwards, target cells 91 in the cell liquid settle to the upper part of the protective liquid under the action of centrifugal force, and the protective liquid, the target cells 91 and the supernatant are sequentially arranged from the cup cone bottom to the cup cover 12, as shown in a graph c in fig. 13;

4) After the centrifugal speed is reduced to a given speed, the supernatant above the constriction 42 is discharged through the second joint 123 and the second pipeline 5, and the residual protective liquid, the target cells 91 and a small amount of supernatant in the cup body 1 are shown as a graph d in fig. 13;

5) The residual liquid in the mixing cup 1 is blown through the first joint 122 and the first pipe 3 and collected.

Embodiment two:

Fig. 5 shows an embodiment of the cell separation device of the present utility model, which includes a rotating frame 6 and a cell centrifugation container according to the first embodiment, wherein the body 1 is rotatably disposed on the rotating frame 6, a vertically rotatable swing cover 7 is disposed on the rotating frame 6, and the body 1 is sleeved in the swing cover 7. The rotating frame 6 rotates to drive the body 1 to rotate for centrifugal movement. The cell separation device comprises all technical characteristics of the cell centrifugation container, and has all advantages of the cell centrifugation container.

Further, a plurality of cell centrifugation containers are symmetrically arranged on the rotating frame 6, and when the rotating frame 6 is static, the body 1 is in a vertical state under the action of gravity, namely, the center line of the shrinkage cavity 41 is in a vertical state. When the rotating frame 6 rotates, the body 1 rotates around the swing axis to be in a horizontal state, i.e., the center line of the contraction cavity 41 is in a horizontal state, due to centrifugal force.

Embodiment III:

Fig. 5 shows an embodiment of the cell separation system according to the present utility model, the cell separation system according to the present embodiment includes a separation chamber 8 with an opening at the top, the cell separation device according to the second embodiment is provided in the separation chamber 8, a rotation driving mechanism for driving the rotation frame 6 of the cell separation device to rotate is provided on the separation chamber 8, a cover plate 81 is provided at the opening of the separation chamber 8, a control module 82 and a hanging frame 83 for hanging a liquid supply device are provided on the separation chamber 8, both the liquid supply device and the rotation driving mechanism are connected with the control module 82, and the separation chamber 2 is connected with the liquid supply device through a conveying pipe. The centrifugal movement of the cell separation apparatus is performed in the separation chamber 8, so that the influence of noise on the external environment can be reduced, and no substances are thrown out. The cell separation system comprises all technical characteristics of the cell separation device and has all advantages of the cell separation device.

Embodiment four:

fig. 9 to 12 show an embodiment of the cell separation method of the present utility model, which is carried out using the cell centrifugation container of embodiment one, comprising the steps of:

S1, adding target cells 91 and a separating liquid 92: adding a cell solution 9 with target cells 91 and a separating solution 92 into the separating cavity 2 through the first pipeline 3, so that the separating solution 92 is below the cell solution 9; when the target cells 91 and the separating liquid 92 are added, the body 1 is in a vertical state, i.e., the conical tip of the contraction cavity 41 is downward.

S2, centrifugal delamination: the body 1 centrifugally moves, the cell liquid 9 separates out target cells 91 under the action of centrifugal force, the target cells 91 are positioned in the contraction cavity 41, and the separation liquid 92 is arranged between the target cells 91 and the cone tip part of the separation cavity 2;

S3, taking out target cells 91: the target cells 91 in the contraction cavity 41 are taken out through the second pipe 5.

The separated target cells 91 are positioned in the contraction cavity 41, the contact rate of the relative volume of the contact surface of the separated target cells and the side wall of the contraction cavity 41 is small, and the yield of the target cells is high; when collecting the target cells, the second pipe 5 extending to the constriction opening 42 is used, and the area of the surface of the constriction chamber 41 corresponding to the bottom surface of the second pipe 5 is close to or equal to the area of the constriction opening 42, so that the target cells can be collected more sufficiently with a small area, and the yield of the cells can be improved.

Further, the addition of the cell liquid 9 (e.g., blood) with the target cells 91 and the separating liquid 92 is performed in a state where the body 1 is stationary, and the cup 1 is placed with the cone tip down. Through the first joint 122, the first pipe 3 is slowly added with the separating liquid 92 to the conical bottom of the cup body 1, and the separating liquid 92 forms a layer with the blood above.

Further, when the cup body 1 is in a state that the cone tip faces outwards during centrifugation and layering, non-target cells (red blood cells) in the cell liquid 9 sink to the bottom of the cup body 1 under the action of centrifugal force, target cells 91 in blood sink to the upper surface of the separating liquid 92 under the action of centrifugal force, and the non-target cells, the separating liquid 92, the target cells 91 and the supernatant are sequentially arranged from the bottom to the top of the cup, as shown in fig. 10. The target cell extraction is performed after the centrifugation speed is reduced to a given speed, specifically: the non-target cells and part of the separating liquid 92 at the bottom of the cup are discharged through the first joint 122 and the first pipeline 3, so that the target cells 91 are ensured to be positioned above the contraction opening 42, as shown in fig. 11; and collecting a small amount of separating liquid 92, target cells 91 and a small amount of supernatant above the constriction 42 through the second joint 123 and the second pipeline 5, and collecting residual separating liquid 92 at the bottom of the cone and residual supernatant above the constriction 42 after the collection of the residual separating liquid 92 at the bottom of the cone of the cup body 1 is completed, as shown in fig. 12. Finally, the residual liquid in the cup 1 is discharged through the first joint 122 and the first pipe 3.

The method comprises the following specific steps:

1) In a static state, the cup body 1 is positioned with the cone tip facing downwards. Blood is added to the cup 1 through the first connector 122, the first tubing 3, as shown in fig. 9;

2) Separating liquid 92 is slowly added to the conical bottom of the cup body 1 through the first joint 122 and the first pipeline 3, and the separating liquid 92 and the upper blood form a layer, as shown in fig. 9;

3) The cup body 1 is in a cone tip outward state, red blood cells in blood sink to the cone bottom of the cup body 1 under the action of centrifugal force, target cells 91 in blood sink to the upper surface of the separating liquid 92 under the action of centrifugal force, and red blood cells, the separating liquid 92, the target cells 91 and supernatant are sequentially arranged from the cup cone bottom to the cup cover 12, as shown in fig. 10;

4) After the centrifugal speed is reduced to a given speed, the red blood cells at the bottom of the cup and part of the separating liquid 92 are discharged through the first joint 122 and the first pipeline 3, so that the target cells 91 are ensured to be positioned above the contraction opening 42, as shown in fig. 11;

5) Collecting a small amount of separating liquid 92, target cells 91 and a small amount of supernatant above the constriction 42 through the second joint 123 and the second pipeline 5, and after the collection, collecting residual separating liquid 92 at the bottom of the cup 1 and residual supernatant above the constriction 42, as shown in fig. 12;

6) Residual liquid in the cup 1 is discharged through the first joint 122 and the first pipe 3.

Fifth embodiment:

FIG. 13 shows an embodiment of the cell separation method of the present utility model, performed using the cell centrifugation container of embodiment one, comprising the steps of:

1) In a static state, the cup body 1 is positioned with the cone tip facing downwards. Adding cell sap to the cup 1 through the first joint 122 and the first pipe 3 as shown in a diagram of fig. 13;

2) Slowly adding a protective liquid (separating liquid 92) to the conical bottom of the cup body 1 through the first joint 122 and the first pipeline 3, wherein the protective liquid and the cell liquid above form layering, as shown in a diagram b in fig. 13;

3) The cup body 1 is in a state that the cone tip faces outwards, target cells 91 in the cell liquid settle to the upper part of the protective liquid under the action of centrifugal force, and the protective liquid, the target cells 91 and the supernatant are sequentially arranged from the cup cone bottom to the cup cover 12, as shown in a graph c in fig. 13;

4) After the centrifugal speed is reduced to a given speed, the supernatant above the constriction 42 is discharged through the second joint 123 and the second pipeline 5, and the residual protective liquid, the target cells 91 and a small amount of supernatant in the cup body 1 are shown as a graph d in fig. 13;

5) The residual liquid in the mixing cup 1 is blown through the first joint 122 and the first pipe 3 and collected.

While the utility model has been described in terms of preferred embodiments, it is not intended to be limiting. Many possible variations and modifications of the disclosed technology can be made by anyone skilled in the art, or equivalent embodiments with equivalent variations can be made, without departing from the scope of the utility model. Therefore, any simple modification, equivalent variation and modification of the above embodiments according to the technical substance of the present utility model shall fall within the scope of the technical solution of the present utility model.

Claims (9)

1. The utility model provides a cell centrifugation container, includes body (1), locates separation chamber (2) and extends to first pipeline (3) of separation chamber (2) bottom in body (1), its characterized in that: the cell centrifugation container is characterized in that a contraction part (4) is arranged in the separation cavity (2), a conical contraction cavity (41) is formed in the contraction part (4), the conical tip part of the contraction cavity (41) faces the bottom of the separation cavity (2) and is provided with a contraction opening (42) communicated with the bottom of the separation cavity (2), and the cell centrifugation container further comprises a second pipeline (5) extending to the contraction opening (42).

2. The cell centrifugation container according to claim 1, wherein: the end face of the contraction part (4) facing the bottom of the separation cavity (2) is a conical surface with a conical tip part connected with the contraction opening (42).

3. The cell centrifugation container according to claim 1, wherein: the outer side wall of the shrinkage part (4) is in fit connection with the inner wall of the separation cavity (2), a collection cavity (21) is arranged at the bottom of the separation cavity (2), and the first pipeline (3) extends to the collection cavity (21).

4. The cell centrifugation container according to claim 1, wherein: the separation cavity (2) comprises a conical part, and the bottom of the separation cavity (2) is positioned at the conical tip part of the conical part.

5. The cell centrifugation container according to claim 1, wherein: the body (1) comprises a centrifugal cup (11) and a cup cover (12), the separation cavity (2) is formed in the centrifugal cup (11), and the cup cover (12) covers an opening of the centrifugal cup (11).

6. The cell centrifugation container according to claim 5, wherein: the cup cover (12) is provided with a ventilation membrane (121), the first pipeline (3) penetrates through the middle of the cup cover (12), and the second pipeline (5) is coaxially sleeved with the first pipeline (3).

7. The cell centrifugation container according to claim 6, wherein: the cup cover (12) is provided with a first connector (122) and a second connector (123), the first pipeline (3) is connected with the first connector (122), and the second pipeline (5) is connected with the second connector (123).

8. A cell separation device, characterized in that: the cell centrifugation container comprises a rotating frame (6) and any one of claims 1 to 7, wherein the body (1) is rotatably arranged on the rotating frame (6), a swinging sleeve (7) capable of vertically rotating is arranged on the rotating frame (6), and the body (1) is sleeved in the swinging sleeve (7).

9. A cell separation system, characterized in that: including open-top's separation chamber (8), be equipped with in separation chamber (8) the cell separation device of claim 8, be equipped with the rotatory actuating mechanism of swivel mount (6) that are used for driving cell separation device on separation chamber (8), the opening part of separation chamber (8) is equipped with apron (81), be equipped with control module (82) on separation chamber (8) and be used for hanging rack (83) of putting the feed liquid device, feed liquid device and rotatory actuating mechanism all are connected with control module (82), separation chamber (2) are connected with feed liquid device through pipeline.