CN211946981U

CN211946981U - Stem cell humidifying centrifugal equipment

Info

Publication number: CN211946981U
Application number: CN202020250463.4U
Authority: CN
Inventors: 王娟; 田程; 张大军; 肖睿娟; 李高卫
Original assignee: Zhengzhou Aobo Cell Medicine Laboratory Co ltd
Current assignee: Zhengzhou Aobo Cell Medicine Laboratory Co ltd
Priority date: 2020-03-04
Filing date: 2020-03-04
Publication date: 2020-11-17
Anticipated expiration: 2030-03-04

Abstract

Stem cell humidification centrifugal device, the load simulator comprises a case, machine incasement portion has the backup pad, be equipped with driving motor in the lower chamber, install the pivot in the backup pad, the top of pivot is equipped with puts the thing board, the inside of putting the thing board is equipped with the cavity, the top of cavity is equipped with and has passed the screw rod in nut and the nut, fixed first ejector pad on the screw rod, the inside of putting the thing board is equipped with the through-hole, be equipped with the connecting rod in the through-hole, all be equipped with the roof in a plurality of test tube holes, be equipped with in the cavity second ejector pad and a plurality of second ejector pad of a plurality of quantity and connecting rod quantity one-. Can stabilize simultaneously a plurality of test tubes when carrying out the during operation, increase production efficiency, avoid the test tube to rock and produce the influence to the stem cell separation, have the humidification effect simultaneously, further improvement stem cell's separation effect.

Description

Stem cell humidifying centrifugal equipment

Technical Field

The utility model relates to a stem cell experimental facilities technical field especially relates to stem cell humidification centrifugal equipment.

Background

Hematopoietic Stem Cells (HSCs) refer to stem cells in the bone marrow that have the ability to self-renew and differentiate into various blood cell precursors, ultimately producing various blood cell components including red blood cells, white blood cells and platelets, which can also differentiate into various other cells. They have good differentiation and proliferation ability, and stem cells can help many people with blood diseases, most commonly leukemia. The hemopoietic stem cell is mainly separated from umbilical cord blood of a newborn and comprises the following steps: (1) a sterile syringe was used to add a proportion of the hydroxyethyl starch solution to the blood. (2) The blood was thoroughly mixed using an automatic rocker. (3) After the mixture is mixed, placing the mixture in an environment with the temperature of 4 ℃ for 1500r/min for centrifugation; (4) and removing the waste plasma layer and the red blood cell layer after the centrifugation is finished, and keeping the leucocyte layer for cell counting. The operation of centrifugation needs to be used to foretell in-process, generally need put into centrifugal equipment with a plurality of test tubes simultaneously when the centrifugation and carry out centrifugal treatment, but at present in placing centrifugal equipment with the test tube, and not fixing the test tube, lead to having the shake in the centrifugation, influence centrifugal effect, current centrifugal equipment probably has the structure of fixed test tube, but need carry out centrifugal operation with a plurality of test tubes simultaneously when the centrifugation, current equipment can not realize fixing simultaneously to a plurality of test tubes, thereby efficiency when having influenced the centrifugation. And the centrifugal device does not control the internal humidity during centrifugation, which not only affects the separation effect of stem cells, but also has a certain effect on the activity of cells.

SUMMERY OF THE UTILITY MODEL

To the above situation, for overcoming the defect of prior art, the utility model aims at providing a stem cell humidification centrifugal equipment, can fix simultaneously a plurality of test tubes when carrying out centrifugal operation, improve production efficiency to control the inside humidity of centrifugal equipment, improve stem cell's separation effect.

The stem cell humidifying centrifugal equipment comprises a cylindrical case, wherein a round supporting plate is fixed inside the case and coaxially installed between the supporting plate and the case, the supporting plate divides the inside of the case into an upper chamber and a lower chamber, a driving motor is arranged in the lower chamber, a rotating shaft is rotatably installed on the supporting plate and coaxially installed with the case, one end of the rotating shaft is positioned in the lower chamber, the other end of the rotating shaft is positioned between the output end of the driving motor and the output end of the lower chamber, a round object placing plate is arranged above the rotating shaft and coaxially installed between the object placing plate and the rotating shaft, a cylindrical cavity is arranged inside the object placing plate and coaxially arranged between the cavity and the object placing plate, a nut fixed on the object placing plate is arranged above the cavity, a screw rod penetrates through the nut, the screw rod is in threaded fit with the nut, one end of the screw rod is positioned, the utility model discloses a test tube, including a plurality of test tube holes, put the inside of thing board and be equipped with many and put the quantity one-to-one of thing board axis looks vertically through-hole and the quantity one-to-one of test tube hole, the one end of a plurality of through-holes all communicates with the cavity rather than communicating between the test tube hole that corresponds between the one end of a plurality of through-holes and a plurality of through-hole keep away from the one end of test tube hole, all be equipped with the connecting rod in a plurality of test tube holes respectively, the one end that the one end of a plurality of connecting rods is located rather than the test tube hole that corresponds and a plurality of connecting rods keep away from the one end of test tube hole all is located the cavity, all be equipped with roof and a plurality of roof respectively rather than corresponding and the connecting rod between fixed connection, the machine case top is rotated and is equipped with a plurality of universal wheels below circular shape case lid.

Preferably, the case cover is provided with a handle and a sealing surface, and the sealing surface is positioned on one surface close to the inside of the case.

Preferably, all overlap on a plurality of connecting rods and have the spring and the spring is located the ejector pad and puts between the thing board, fixed connection between one end of spring and the second ejector pad and the spring keep away from the one end of ejector pad and put fixed connection between the thing board.

Preferably, the top plate is arc-shaped, the direction of the arc faces the direction departing from the connecting rod, and the top plate is provided with an anti-skid surface which is positioned on one surface departing from the connecting rod.

Preferably, the second pushing block is provided with an inclined surface, the inclined surface is positioned on one side far away from the connecting rod, and the inclined direction of the inclined surface faces one side of the connecting rod.

Preferably, a humidifier is fixed on the case, and an outlet of the humidifier is communicated with the case.

The utility model has the advantages that: can be after placing the test tube downthehole portion, can realize fixing simultaneously a plurality of test tubes, convenient and fast has increased production efficiency, has increased the stability when stem cell centrifugation simultaneously, has improved stem cell separation effect. Realize fixing a plurality of test tubes in step through nut and screw rod structure, improved production efficiency. And the humidifier is fixed on the case, and when centrifugation is carried out, the humidifier can humidify the inside of the case, so that the effect of stem cell separability is further improved.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention.

Fig. 2 is a schematic structural view of the object placing plate of the present invention.

Fig. 3 is a schematic structural diagram of the whole cavity of the present invention.

Detailed Description

The following describes the embodiments of the present invention in further detail with reference to the accompanying drawings.

As shown in the figures 1, 2 and 3, the stem cell humidifying centrifugal device comprises a cylindrical case 1 and is characterized in that a circular support plate 2 is fixed inside the case 1, the support plate 2 and the case 1 are coaxially installed, the support plate 2 divides the inside of the case 1 into an upper chamber 3 and a lower chamber 4, a driving motor 5 is arranged in the lower chamber 4, a rotating shaft 6 is rotatably installed on the support plate 2, the rotating shaft 6 and the case 1 are coaxially installed, one end of the rotating shaft 6 is located in the lower chamber 4, the rotating shaft 6 is located between one end of the lower chamber 4 and an output end of the driving motor 5, a circular object placing plate 7 is arranged above the rotating shaft 6, the object placing plate 7 and the rotating shaft 6 are coaxially installed, a cylindrical cavity 19 is arranged inside the object placing plate 7, the cavity 19 and the object placing plate 7 are coaxially arranged, a nut 9 fixed on the object placing plate 7 is arranged above the, screw-thread fit between screw rod 8 and nut 9, screw rod 8's one end is located cavity 19 and screw rod 8 is located cavity 19 inside one and serves and be fixed with conical first ejector pad 10, the inside of putting thing board 7 is equipped with many and put thing board 7 axis to vertically through-hole 14 and the quantity one-to-one of through-hole 14 and test tube hole 11, the one end of a plurality of through-holes 14 communicates respectively rather than between the test tube hole 11 that corresponds and the one end that test tube hole 11 was kept away from to a plurality of through-holes 14 all communicates with cavity 19, all be equipped with connecting rod 13 in a plurality of through-holes 14, the one end of a plurality of connecting rods 13 is located rather than corresponding test tube hole 11 respectively and the one end that test tube hole 11 was kept away from to a plurality of connecting rods 13 all is located cavity 19, all be equipped with roof 12 and a plurality of roof 12 respectively in a plurality of test tube holes 11 rather than corresponding and be fixed connection with connecting rod 13 between, be equipped with a plurality of quantity and the second ejector pad 16 and a plurality of second ejector pad 16 that And the corresponding connecting rods 13 are fixedly connected, a round box cover 20 is rotatably arranged above the case 1, and a plurality of universal wheels 17 are arranged below the case 1.

When the device is used, firstly, the device is placed at a position needing to work, then the box cover 20 is opened, a plurality of test tubes are placed in the test tube holes 11 on the object placing plate 7, then the screw rod 8 is rotated, when the screw rod 8 rotates, the nut 9 is fixed on the object placing plate 7, the screw rod 8 can move up and down through the thread fit between the screw rod 8 and the nut 9, the screw rod 8 can drive the first push block 10 fixed on the screw rod 8 to move up and down when moving up and down, the first push block 10 is conical, the second push block 16 can be moved in the process of moving up and down of the first push block 10, when the second push block 16 moves down, the radius of the first push block when contacting with the second push block 16 is continuously increased, so that the second push block 16 moves towards the through hole 14, the connecting rod 13 is fixed on the second push block 16, connecting rod 13 is located through-hole 14, under the effect of through-hole 14, connecting rod 13 can only move along through-hole 14's direction, when second ejector pad 16 removes to through-hole 14's direction, connecting rod 13 has been realized removing to test tube hole 11 direction, thereby make roof 12 fixed on connecting rod 13 remove to the direction of keeping away from through-hole 14, thereby realized roof 12 and constantly removed, thereby test tube hole 11's internal diameter has been reduced, thereby realized the reinforcement to the test tube, because be equipped with a plurality of ejector pads in the cavity 19, and the ejector pad all is connected with connecting rod 13 and roof 12, realized fixing a plurality of test tubes simultaneously. The time of fixing the test tube is reduced, and meanwhile, the problem that the test tube is not fixed due to manual errors is avoided, so that the production efficiency is increased, the test tube is fixed, and the effect of stem cell separation is increased. Adopt screw rod 8 and nut 9 to operate, have self-locking function, stability when can improve the test tube fixing, further improvement stem cell separation's effect. At this moment, the box cover 20 is closed, then the driving motor 5 is opened, the driving motor 5 rotates to drive the rotating shaft 6 to rotate, so that the rotating shaft 6 drives the object placing plate 7 to rotate, and the stem cell volume centrifugal operation in the test tube is realized. After the centrifugation is finished, the box cover 20 is opened, then the screw 8 is rotated in the opposite direction, so that the test tubes are released simultaneously, the experiment time is reduced, and the use is convenient.

The case cover 20 is provided with a handle and the case cover 20 is provided with a sealing surface, and the sealing surface is positioned on one surface close to the inside of the case 1.

In order to avoid that the outside air enters the inside of the case 1 during centrifugation and affects the stem cell separation effect, a sealing surface is provided on the case cover 20, so that the sealing performance of the case cover 20 during closing is improved, and the outside air is prevented from entering the inside of the case 1. A handle on the lid 20 facilitates lifting of the lid 20.

All overlap on a plurality of connecting rods 13 and have spring 15 and spring 15 to be located the ejector pad and put between the thing board 7, fixed connection between one end of spring 15 and the second ejector pad 16 and spring 15 keep away from the one end of ejector pad and put between the thing board 7 fixed connection.

In order to make the screw 8 rotate in the opposite direction, the connecting rod 13 can drive the top plate 12 to move towards the through hole 14, so that the test tube can be automatically released, and meanwhile, the situation that the top plate 12 is located at the center of the test tube hole 11 when in use, so that the test tube needs to be moved by hand is avoided, and the convenience in use is improved.

The top plate 12 is arc-shaped, the direction of the arc faces the direction departing from the connecting rod 13, and the top plate 12 is provided with an anti-skid surface which is located on the surface departing from the connecting rod 13.

Because the test tube is cylindrical mostly, in order to improve the tight degree of clamp of roof 12 to the test tube, set roof 12 to circular-arcly, improved roof 12 area of contact when contacting with the test tube lateral wall, increased the stability when pressing from both sides tight the test tube to the steadiness of test tube has been improved, and set up the non-skid surface on the arc surface, further improvement roof 12 is to the power of grabbing of test tube.

The second push block 16 is provided with an inclined surface, the inclined surface is located on one side far away from the connecting rod 13, and the inclined direction of the inclined surface faces one side of the connecting rod 13.

In order to increase the smoothness of the first push block 10 in pushing the second push block 16, the second push block 16 is provided with an inclined surface, and the inclined surface is located on the side in contact with the first push block 10, so that the smoothness of the force applied to the second push block 16 by the second push block 16 when the second push block 16 moves is improved, meanwhile, the contact area between the second push block 16 and the second push block 16 is increased, and the stability of the force applied to the second push block 16 by the first push block 10 is further increased.

A humidifier 21 is fixed on the case 1, and an outlet of the humidifier 21 is communicated with the case 1.

In order to avoid the influence of the humidity of the air inside the case 1 on the stem cell centrifugation effect during centrifugation, the humidifier 21 is fixed on the case 1, and the humidifier 21 is opened during centrifugation, so that the humidifier 21 can humidify the inside of the case 1 continuously, the stability during stem cell centrifugation is increased, and the stem cell centrifugation effect is improved.

The above-mentioned embodiments are not intended to limit the scope of the present invention, and various modifications and improvements made by those skilled in the art without departing from the design concept of the present invention should be included in the protection scope defined by the claims of the present invention.

Claims

1. Stem cell humidification centrifugal device, including columniform quick-witted case (1), its characterized in that, quick-witted case (1) inside is fixed with circular shape backup pad (2) and quick-witted case (1) between coaxial line installation, backup pad (2) with quick-witted case (1) internal partitioning be last cavity (3) and lower cavity (4) and be equipped with driving motor (5) down in cavity (4), rotate on backup pad (2) and install pivot (6) and quick-witted case (1) coaxial line installation, the one end of pivot (6) is located down cavity (4) and pivot (6) are located and are connected between the one end of cavity (4) and driving motor (5) the output, the top of pivot (6) is equipped with circular shape and puts thing board (7) and put between thing board (7) and pivot (6) coaxial line installation, it is equipped with cylindrical cavity (19) and puts and establishes the coaxial line between thing board (7) to put the inside of thing board (7) to put thing Put, the top of cavity (19) is equipped with nut (9) and nut (9) that are fixed in on putting thing board (7) and has passed screw rod (8) in, screw-thread fit between screw rod (8) and nut (9), and the one end of screw rod (8) is located cavity (19) and screw rod (8) are located cavity (19) inside one end and are fixed with conical first ejector pad (10), put the inside of thing board (7) and be equipped with many and put thing board (7) axis looks vertically through-hole (14) and the quantity one-to-one of through-hole (14) and the quantity of test tube hole (11), the one end of a plurality of through-holes (14) communicates between test tube hole (11) rather than corresponding respectively and a plurality of through-holes (14) keep away from the one end of test tube hole (11) and all communicate with cavity (19), all be equipped with connecting rod (13) in a plurality of through-holes (14), the one end of a plurality of connecting rod (13) is located in test tube hole (11) rather than corresponding respectively and a plurality of connecting rod (13) keep away from the ) The one end of all being located cavity (19), all be equipped with roof (12) and a plurality of roof (12) respectively rather than correspond in a plurality of test tube holes (11) and connecting rod (13) between fixed connection, be equipped with in cavity (19) a plurality of quantity and connecting rod (13) quantity one-to-one second ejector pad (16) and a plurality of second ejector pad (16) respectively rather than corresponding between fixed connection between connecting rod (13), the below of installing circular shape case lid (20) and quick-witted case (1) is rotated to quick-witted case (1) top is equipped with a plurality of universal wheels (17).

2. Stem cell humidification centrifuge as claimed in claim 1 wherein the lid (20) is provided with a handle and the lid (20) is provided with a sealing surface on a side adjacent to the interior of the housing (1).

3. The stem cell humidifying and centrifuging device according to claim 1, wherein the plurality of connecting rods (13) are sleeved with springs (15), the springs (15) are located between the pushing block and the object placing plate (7), one ends of the springs (15) are fixedly connected with the second pushing block (16), and one ends of the springs (15) far away from the pushing block are fixedly connected with the object placing plate (7).

4. The stem cell humidifying and centrifuging device according to claim 1, wherein the top plate (12) is arc-shaped and the direction of the arc faces the direction away from the connecting rod (13), and the top plate (12) is provided with an anti-slip surface which is located on the surface away from the connecting rod (13).

5. The stem cell humidifying centrifugal device according to claim 1, wherein the second push block (16) is provided with an inclined surface and the inclined surface is located at a side far from the connecting rod (13), and the inclined surface is inclined towards the side of the connecting rod (13).

6. The stem cell humidifying centrifugal device according to claim 1, wherein a humidifier (21) is fixed on the case (1) and an outlet of the humidifier (21) is communicated with the case (1).