CN113046302A - Stem cell separation method - Google Patents
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- CN113046302A CN113046302A CN202110329050.4A CN202110329050A CN113046302A CN 113046302 A CN113046302 A CN 113046302A CN 202110329050 A CN202110329050 A CN 202110329050A CN 113046302 A CN113046302 A CN 113046302A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
- C12N2509/10—Mechanical dissociation
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Abstract
The invention relates to the field of cell separation, in particular to a stem cell separation method, which comprises the following steps: step one, adding stem cell suspension into a centrifuge dish filled with a culture medium; step two, after the centrifugal vessel is sealed, a motor is started to drive the centrifugal vessel to rotate; step three, rotating to separate, and taking out the cells to complete separation; the stem cell separation method also relates to a stem cell separation device; a stem cell separator include main shaft, limit slide, linkage cover, rotatory diaphragm, slide opening, mount pad, installation axle and centrifugation ware, the upper end of main shaft is equipped with limit slide, linkage cover sliding connection on the main shaft, and limit slide runs through the linkage cover, rotatory diaphragm fixed connection is equipped with the slide opening in the upper end of linkage cover, is equipped with the slide opening on the rotatory diaphragm in the slide opening, installation axle sliding connection in the slide opening, the centrifugation ware rotates to be connected in the upper end of installation axle.
Description
Technical Field
The invention relates to the field of cell separation, in particular to a stem cell separation method.
Background
Stem cells refer to a class of cells from embryos, fetuses, and adults with the ability to self-renew and to multiply and differentiate into various cells, and are the initial source of cells of organisms and other various tissues, and these cells can differentiate into various different tissue cells, which are poor and lacking cell sources in the biomedical field, and are of great significance for clinical application, so that the research of stem cells in recent years is the hot spot of biomedical research. When stem cells are separated, centrifugal equipment is often needed to separate cell suspension, and the existing equipment is low in separation efficiency and inconvenient to use.
Disclosure of Invention
The invention aims to provide a stem cell separation method, which has the following beneficial effects: the method relates to a stem cell separation device, which has high separation efficiency and is convenient to use.
The purpose of the invention is realized by the following technical scheme:
a method of stem cell isolation comprising the steps of:
step one, adding stem cell suspension into a centrifuge dish filled with a culture medium;
step two, after the centrifugal vessel is sealed, a motor is started to drive the centrifugal vessel to rotate;
step three, rotating to separate, and taking out the cells to complete separation;
the stem cell separation method also relates to a stem cell separation device;
a stem cell separator include main shaft, limit slide, linkage cover, rotatory diaphragm, slide opening, mount pad, installation axle and centrifugation ware, the upper end of main shaft is equipped with limit slide, linkage cover sliding connection on the main shaft, and limit slide runs through the linkage cover, rotatory diaphragm fixed connection is equipped with the slide opening in the upper end of linkage cover, is equipped with the slide opening on the rotatory diaphragm in the slide opening, installation axle sliding connection in the slide opening, the centrifugation ware rotates to be connected in the upper end of installation axle.
The stem cell separation device further comprises a power motor, a spring I and a linkage rod, wherein the power motor is arranged at the lower end of the main shaft, the spring I is fixedly connected to the upper end of the main shaft, the upper end of the spring I tightly pushes against the rotary transverse plate, and two ends of the linkage rod are respectively connected with the limiting slide block and the mounting seat in a rotating mode.
The stem cell separation device further comprises an installation pipe, a locking block, a locking rod, a locking spring and a locking groove, wherein the sliding hole is far away from the main shaft end and is provided with the installation pipe, the locking rod is connected in the installation pipe in a sliding mode, the locking rod is close to the main shaft end and is provided with the locking block, the other end of the locking rod is provided with the locking spring, the locking spring pushes the sliding hole tightly to be far away from the main shaft end, the installation shaft is provided with the locking groove, the locking rod penetrates through the installation seat, and the.
A groove is formed in the mounting seat and used for shrinkage of the locking block.
The stem cell separation device further comprises a cover plate, and the cover plate is connected to the upper end of the centrifugal dish in a sliding mode.
A stem cell separator still include locking otic placode, fixed platelet, the control panel, lockpin and little spring, the upper end symmetry of centrifugation ware is equipped with locking otic placode, the upper end symmetry of apron is equipped with fixed otic placode, equal sliding connection has the lockpin in two fixed otic placodes, two lockpins respectively with two locking otic placode sliding connection, the equal fixedly connected with control panel in the inner of two lockpins, the equal fixedly connected with little spring in the inner of two control panels, the inner of two little springs is through respectively through two fixed platelet fixed connection on the apron.
The outer ends of the two lock pins are hemispherical.
The stem cell separation device further comprises a supporting base, a toothed ring, a linkage shaft and a rotating wheel, the lower end of the main shaft is connected to the center of the supporting base in a rotating mode, the toothed ring is arranged at the upper end of the supporting base, the rotating transverse plate and the sliding hole are far away from the end and are connected with the linkage shaft in a rotating mode, the linkage shaft is connected with the rotating wheel in a sliding mode through keys, and the rotating wheel is connected with the toothed ring in a meshing transmission mode.
The stem cell separation device further comprises a rotating plate, a tension spring and a driving wheel, wherein one end of the rotating plate is rotatably connected to a linkage shaft, the driving wheel is rotatably connected to the other end of the rotating plate, the linkage shaft is in transmission connection with the driving wheel through a belt, one end of the tension spring is fixedly connected with the rotating plate, the other end of the tension spring is fixedly connected with a rotating transverse plate, and the driving wheel is in rolling connection with a centrifugal dish.
The stem cell separation device further comprises a thread small plate and a screw rod, the thread small plate is fixedly connected to the rotary transverse plate, the screw rod is connected to the thread small plate through threads, and the screw rod tightly pushes the rotary plate.
The invention has the beneficial effects that: the invention provides a stem cell separation method, relates to a stem cell separation device, and is high in separation efficiency and convenient to use.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a partial schematic view of the first embodiment of the present invention;
FIG. 3 is a second partial schematic structural view of the present invention;
FIG. 4 is a third schematic view of a portion of the present invention;
FIG. 5 is a fourth schematic view of a portion of the present invention;
FIG. 6 is a schematic diagram of a portion of the present invention;
FIG. 7 is a schematic diagram six of a portion of the present invention;
FIG. 8 is a seventh schematic view of a portion of the present invention;
fig. 9 is a partial structural schematic view eight of the present invention.
In the figure: a power motor 101; a main shaft 102; a limiting slide block 103; a spring I104; a linking sleeve 201; rotating the transverse plate 202; a linking shaft 203; a rotating wheel 204; a threaded platelet 205; a slide hole 206; a mounting tube 207; a mount 301; a linkage bar 302; a groove 303; a lock block 401; a locking bar 402; a locking spring 403; mounting a shaft 501; a locking groove 502; a centrifuge dish 503; a locking ear plate 504; a support base 601; a ring gear 602; a cover plate 701; a fixed ear plate 702; fixing the platelet 703; a control panel 704; a locking pin 705; a small spring 706; a rotating plate 801; a screw 802; a tension spring 803; a drive wheel 804.
Detailed Description
The invention is described in further detail below with reference to figures 1-9.
A method of stem cell isolation comprising the steps of:
step one, adding stem cell suspension into a centrifuge dish filled with a culture medium;
step two, after the centrifugal vessel is sealed, a motor is started to drive the centrifugal vessel to rotate;
step three, rotating to separate, and taking out the cells to complete separation;
the stem cell separation method also relates to a stem cell separation device;
the stem cell separation device comprises a main shaft 102, a limiting slide block 103, a linkage sleeve 201, a rotating transverse plate 202, a slide hole 206, an installation seat 301, an installation shaft 501 and a centrifuge dish 503, wherein the limiting slide block 103 is arranged at the upper end of the main shaft 102, the linkage sleeve 201 is slidably connected onto the main shaft 102, the limiting slide block 103 penetrates through the linkage sleeve 201, the rotating transverse plate 202 is fixedly connected to the upper end of the linkage sleeve 201, the slide hole 206 is arranged on the rotating transverse plate 202, the installation seat 301 is slidably connected into the slide hole 206, the installation shaft 501 is slidably connected into the slide hole 206, and the centrifuge dish 503 is rotatably connected to the upper end of the installation shaft 501.
When cell suspension is separated, the cell suspension is poured into a centrifuge dish 503, the main shaft 102 drives the linkage sleeve 201 to rotate through the limiting slide block 103, the linkage sleeve 201 drives the rotation transverse plate 202 to rotate, the rotation transverse plate 202 drives the installation seat 301 to rotate through the slide hole 206, and the installation seat 301 drives the centrifuge dish 503 to rotate by taking the main shaft 102 as a shaft through the installation shaft 501, so that the cell suspension in the centrifuge dish 503 is centrifugally rotated, and the purpose of separating cells is achieved.
The second embodiment is as follows:
as shown in fig. 1-9, the stem cell separation device further comprises a power motor 101, a spring i 104 and a linkage rod 302, the power motor 101 is arranged at the lower end of the main shaft 102, the spring i 104 is fixedly connected to the upper end of the main shaft 102, the upper end of the spring i 104 abuts against the rotating transverse plate 202, and two ends of the linkage rod 302 are rotatably connected with the limiting slide block 103 and the mounting base 301 respectively.
The power motor 101 is used for providing power for the rotation of the spindle 102, through the arrangement of the spring I104 and the linkage rod 302, when the amount of cell suspension added into the centrifuge dish 503 is large, the overall gravity of the centrifuge dish 503 is increased, the weight of the centrifuge dish 503 acts on the spring I104 through the rotating transverse plate 202, so that the rotating transverse plate 202 extrudes the spring I104 to descend, then the linkage rod 302 drives the mounting seat 301 to move towards the end far away from the spindle 102 in the sliding hole 206, and therefore when the spindle 102 rotates at the same rotating speed, the centrifugal force is larger as the distance from the spindle 102 is larger, and the purpose of self-adjusting the centrifugal force is achieved according to the amount of the separated cell suspension.
The third concrete implementation mode:
as shown in fig. 1 to 9, the stem cell separation device further includes a mounting tube 207, a locking block 401, a locking rod 402, a locking spring 403 and a locking groove 502, the mounting tube 207 is disposed at the end of the slide hole 206 away from the main shaft 102, the locking rod 402 is slidably connected in the mounting tube 207, the locking block 401 is disposed at the end of the locking rod 402 close to the main shaft 102, the locking spring 403 is disposed at the other end of the locking rod 402, the locking spring 403 tightly pushes the slide hole 206 away from the main shaft 102, the locking groove 502 is disposed on the mounting shaft 501, the locking rod 402 passes through the mounting seat 301, and the locking block 401 is slidably connected in the locking groove.
The locking block 401 is always pressed against the locking groove 502 by the elastic force of the locking spring 403, thereby ensuring that the mounting shaft 501 does not come off the mounting base 301 when the centrifuge dish 503 rotates about the main shaft 102.
The fourth concrete implementation mode:
as shown in fig. 1 to 9, a groove 303 is formed in the mounting seat 301 for retracting the locking block 401.
When the centrifuge dish 503 needs to be removed, the centrifuge dish 503 is pushed to move in the sliding hole 206 close to the spindle 102 until the locking block 401 slides into the groove 303, the mounting shaft 501 can slide away from the mounting seat 301, so that the purpose of conveniently removing and cleaning the centrifuge dish 503 is achieved, and when the mounting shaft 501 slides into the mounting seat 301, the mounting seat 301 is pushed enough to move by the gravity of the centrifuge dish 503, and the locking block 401 is guaranteed to be tightly propped in the locking groove 502.
The fifth concrete implementation mode:
as shown in FIGS. 1 to 9, the stem cell separation device further includes a cover plate 701, and the cover plate 701 is slidably coupled to the upper end of the centrifuge dish 503.
The cover plate 701 is used for plugging the centrifuge dish 503.
The sixth specific implementation mode:
as shown in fig. 1 to 9, the stem cell separation device further includes locking ear plates 504, fixing ear plates 702, fixing small plates 703, control plates 704, locking pins 705 and small springs 706, the locking ear plates 504 are symmetrically disposed at the upper end of the centrifuge dish 503, the fixing ear plates 702 are symmetrically disposed at the upper end of the cover plate 701, the locking pins 705 are slidably connected in the two fixing ear plates 702, the two locking pins 705 are slidably connected with the two locking ear plates 504, the control plates 704 are fixedly connected at the inner ends of the two locking pins 705, the small springs 706 are fixedly connected at the inner ends of the two control plates 704, and the inner ends of the two small springs 706 are fixedly connected to the cover plate 701 through the two fixing small plates 703.
When the cover plate 701 needs to be taken down, the two control plates 704 are hooked by hands at the same time and are pressed towards the center, the two control plates 704 are moved towards the center at the same time to press the small spring 706 until the two locking pins 705 are separated from the two locking ear plates 504, and then the two control plates 704 can be pulled upwards to pull the cover plate 701 away from the centrifuge dish 503.
The seventh embodiment:
as shown in fig. 1-9, the outer ends of both latches 705 are formed as hemispheres.
To facilitate the cover plate 701 on the centrifuge bowl 503, the two locking pins 705 slide into the two locking lugs 504.
The specific implementation mode is eight:
as shown in fig. 1-9, the stem cell separation device further comprises a supporting base 601, a toothed ring 602, a coupling shaft 203 and a rotating wheel 204, wherein the lower end of the main shaft 102 is rotatably connected to the center of the supporting base 601, the toothed ring 602 is disposed at the upper end of the supporting base 601, the coupling shaft 203 is rotatably connected to the end of the horizontal rotating plate 202 away from the sliding hole 206, the rotating wheel 204 is slidably connected to the coupling shaft 203 through a key, and the rotating wheel 204 is in meshing transmission connection with the toothed ring 602.
The supporting base 601 is used for supporting the device, the toothed ring 602, the linkage shaft 203 and the rotating wheel 204 are arranged, the balance of the rotating transverse plate 202 is achieved, the phenomenon that one side of the rotating transverse plate 202 is influenced by the gravity of the centrifuge dish 503 and tends to incline is avoided, the main shaft 102, the limiting slide block 103 and the linkage sleeve 201 are clamped, the sliding of the linkage sleeve 201 on the main shaft 102 is influenced, meanwhile, the rotating wheel 204 is connected to the linkage shaft 203 in a sliding mode through keys, and the rotating wheel 204 is connected with the toothed ring 602 in a meshing transmission mode.
The specific implementation method nine:
as shown in fig. 1 to 9, the stem cell separation device further includes a rotation plate 801, a tension spring 803 and a driving wheel 804, wherein one end of the rotation plate 801 is rotatably connected to the coupling shaft 203, the driving wheel 804 is rotatably connected to the other end of the rotation plate 801, the coupling shaft 203 is in belt transmission connection with the driving wheel 804, one end of the tension spring 803 is fixedly connected to the rotation plate 801, the other end of the tension spring 803 is fixedly connected to the rotation cross plate 202, and the driving wheel 804 is in rolling connection with the centrifuge dish 503.
When the spindle 102 drives the rotating horizontal plate 202 to rotate, the rotating wheel 204 is in meshing transmission connection with the toothed ring 602, the rotating wheel 204 rotates by taking the spindle 102 as an axis and simultaneously drives the linkage shaft 203 to rotate by taking the linkage shaft 203 as an axis, so that the driving wheel 804 is driven, the driving wheel 804 drives the centrifuge dish 503 to rotate by taking the mounting shaft 501 as an axis, the centrifugal efficiency is improved, and meanwhile, through the arrangement of the tension spring 803, when the position of the centrifuge dish 503 is changed, the driving wheel 804 can be always in contact with the centrifuge dish 503 and can drive the centrifuge dish 503.
The detailed implementation mode is ten:
as shown in fig. 1 to 9, the stem cell separation device further includes a small screw plate 205 and a screw 802, the small screw plate 205 is fixedly connected to the horizontal rotating plate 202, the screw 802 is connected to the small screw plate 205 through a screw, and the screw 802 abuts against the rotating plate 801.
The rotating screw 802 is rotated to push the rotating plate 801 tightly, so that the rotating plate 801 can be pushed to rotate, the driving wheel 804 is separated from the centrifuge bowl 503, and the centrifuge bowl 503 can be conveniently taken and placed.
The invention relates to a stem cell separation method, which has the use principle that: when cell suspension is separated, the cell suspension is poured into a centrifuge dish 503, the main shaft 102 drives the linkage sleeve 201 to rotate through the limiting slide block 103, the linkage sleeve 201 drives the rotation transverse plate 202 to rotate, the rotation transverse plate 202 drives the installation seat 301 to rotate through the slide hole 206, and the installation seat 301 drives the centrifuge dish 503 to rotate by taking the main shaft 102 as a shaft through the installation shaft 501, so that the cell suspension in the centrifuge dish 503 is centrifugally rotated, and the purpose of separating cells is achieved. The power motor 101 is used for providing power for the rotation of the spindle 102, through the arrangement of the spring I104 and the linkage rod 302, when the amount of cell suspension added into the centrifuge dish 503 is large, the overall gravity of the centrifuge dish 503 is increased, the weight of the centrifuge dish 503 acts on the spring I104 through the rotating transverse plate 202, so that the rotating transverse plate 202 extrudes the spring I104 to descend, then the linkage rod 302 drives the mounting seat 301 to move towards the end far away from the spindle 102 in the sliding hole 206, and therefore when the spindle 102 rotates at the same rotating speed, the centrifugal force is larger as the distance from the spindle 102 is larger, and the purpose of self-adjusting the centrifugal force is achieved according to the amount of the separated cell suspension. The locking block 401 is always pressed against the locking groove 502 by the elastic force of the locking spring 403, thereby ensuring that the mounting shaft 501 does not come off the mounting base 301 when the centrifuge dish 503 rotates about the main shaft 102. When the centrifuge dish 503 needs to be removed, the centrifuge dish 503 is pushed to move in the sliding hole 206 close to the spindle 102 until the locking block 401 slides into the groove 303, the mounting shaft 501 can slide away from the mounting seat 301, so that the purpose of conveniently removing and cleaning the centrifuge dish 503 is achieved, and when the mounting shaft 501 slides into the mounting seat 301, the mounting seat 301 is pushed enough to move by the gravity of the centrifuge dish 503, and the locking block 401 is guaranteed to be tightly propped in the locking groove 502. To facilitate the cover plate 701 on the centrifuge bowl 503, the two locking pins 705 slide into the two locking lugs 504. The supporting base 601 is used for supporting the device, the toothed ring 602, the linkage shaft 203 and the rotating wheel 204 are arranged, the balance of the rotating transverse plate 202 is achieved, the phenomenon that one side of the rotating transverse plate 202 is influenced by the gravity of the centrifuge dish 503 and tends to incline is avoided, the main shaft 102, the limiting slide block 103 and the linkage sleeve 201 are clamped, the sliding of the linkage sleeve 201 on the main shaft 102 is influenced, meanwhile, the rotating wheel 204 is connected to the linkage shaft 203 in a sliding mode through keys, and the rotating wheel 204 is connected with the toothed ring 602 in a meshing transmission mode. When the spindle 102 drives the rotating horizontal plate 202 to rotate, the rotating wheel 204 is in meshing transmission connection with the toothed ring 602, the rotating wheel 204 rotates by taking the spindle 102 as an axis and simultaneously drives the linkage shaft 203 to rotate by taking the linkage shaft 203 as an axis, so that the driving wheel 804 is driven, the driving wheel 804 drives the centrifuge dish 503 to rotate by taking the mounting shaft 501 as an axis, the centrifugal efficiency is improved, and meanwhile, through the arrangement of the tension spring 803, when the position of the centrifuge dish 503 is changed, the driving wheel 804 can be always in contact with the centrifuge dish 503 and can drive the centrifuge dish 503. The rotating screw 802 is rotated to push the rotating plate 801 tightly, so that the rotating plate 801 can be pushed to rotate, the driving wheel 804 is separated from the centrifuge bowl 503, and the centrifuge bowl 503 can be conveniently taken and placed.
It is to be understood that the above description is not intended to limit the present invention, and the present invention is not limited to the above examples, and that various changes, modifications, additions and substitutions which are within the spirit and scope of the present invention and which may be made by those skilled in the art are also within the scope of the present invention.
Claims (10)
1. A method for isolating stem cells, comprising: the method comprises the following steps:
step one, adding stem cell suspension into a centrifuge dish filled with a culture medium;
step two, after the centrifugal vessel is sealed, a motor is started to drive the centrifugal vessel to rotate;
step three, rotating to separate, and taking out the cells to complete separation;
the stem cell separation method also relates to a stem cell separation device;
the stem cell separation device comprises a main shaft (102), a limiting slide block (103), a linkage sleeve (201), a rotating transverse plate (202), a sliding hole (206), an installation seat (301), an installation shaft (501) and a centrifugal dish (503), wherein the limiting slide block (103) is arranged at the upper end of the main shaft (102), the linkage sleeve (201) is connected onto the main shaft (102) in a sliding mode, the limiting slide block (103) penetrates through the linkage sleeve (201), the rotating transverse plate (202) is fixedly connected to the upper end of the linkage sleeve (201), the sliding hole (206) is formed in the rotating transverse plate (202), the installation seat (301) is connected into the sliding hole (206) in a sliding mode, the installation shaft (501) is connected into the sliding hole (206) in a sliding mode, and the centrifugal dish (503) is connected to the upper end of.
2. A method of isolating stem cells according to claim 1, wherein: the stem cell separation device further comprises a power motor (101), a spring I (104) and a linkage rod (302), wherein the power motor (101) is arranged at the lower end of the main shaft (102), the spring I (104) is fixedly connected to the upper end of the main shaft (102), the upper end of the spring I (104) abuts against a rotating transverse plate (202), and two ends of the linkage rod (302) are rotatably connected with a limiting slide block (103) and a mounting seat (301) respectively.
3. A method of isolating stem cells according to claim 2, wherein: the stem cell separation device further comprises an installation pipe (207), a locking block (401), a locking rod (402), a locking spring (403) and a locking groove (502), the end, far away from the main shaft (102), of the sliding hole (206) is provided with the installation pipe (207), the locking rod (402) is connected in the installation pipe (207) in a sliding mode, the end, close to the main shaft (102), of the locking rod (402) is provided with the locking block (401), the other end of the locking rod (402) is provided with the locking spring (403), the locking spring (403) tightly pushes the sliding hole (206) away from the end of the main shaft (102), the locking groove (502) is arranged on the installation shaft (501), the locking rod (402) penetrates through the installation seat (301), and the locking block (401) is connected in.
4. A method of isolating stem cells according to claim 3, wherein: a groove (303) is formed in the mounting seat (301) and used for enabling the locking block (401) to contract.
5. A method of isolating stem cells according to claim 4, wherein: the stem cell separation device also comprises a cover plate (701), wherein the cover plate (701) is connected to the upper end of the centrifuge dish (503) in a sliding mode.
6. A method of isolating stem cells according to claim 5, wherein: the stem cell separation device further comprises a locking lug plate (504), a fixing lug plate (702), a fixing small plate (703), a control plate (704), a locking pin (705) and a small spring (706), the locking lug plate (504) is symmetrically arranged at the upper end of the centrifugal dish (503), the fixing lug plate (702) is symmetrically arranged at the upper end of the cover plate (701), the locking pin (705) is connected in the two fixing lug plates (702) in a sliding mode, the two locking pins (705) are respectively connected with the two locking lug plates (504) in a sliding mode, the control plate (704) is fixedly connected at the inner ends of the two locking pins (705), the small spring (706) is fixedly connected at the inner ends of the two control plates (704), and the inner ends of the two small springs (706) are fixedly connected to the cover plate (701) through the two fixing small plates (703.
7. A method of isolating stem cells according to claim 6, wherein: the outer ends of the two locking pins (705) are both provided with hemispheres.
8. A method of isolating stem cells according to claim 6, wherein: the stem cell separation device further comprises a supporting base (601), a toothed ring (602), a linkage shaft (203) and a rotating wheel (204), the lower end of the main shaft (102) is rotatably connected to the center of the supporting base (601), the toothed ring (602) is arranged at the upper end of the supporting base (601), the linkage shaft (203) is rotatably connected to the end, away from the sliding hole (206), of the rotating transverse plate (202), the rotating wheel (204) is slidably connected to the linkage shaft (203) through keys, and the rotating wheel (204) is in meshing transmission connection with the toothed ring (602).
9. A method of isolating stem cells according to claim 8, wherein: the stem cell separation device further comprises a rotating plate (801), a tension spring (803) and a driving wheel (804), one end of the rotating plate (801) is rotatably connected to the linkage shaft (203), the driving wheel (804) is rotatably connected to the other end of the rotating plate (801), the linkage shaft (203) is in transmission connection with the driving wheel (804) through a belt, one end of the tension spring (803) is fixedly connected with the rotating plate (801), the other end of the tension spring (803) is fixedly connected with the rotating transverse plate (202), and the driving wheel (804) is in rolling connection with the centrifugal dish (503).
10. A method of isolating stem cells according to claim 9, wherein: the stem cell separation device further comprises a small threaded plate (205) and a screw rod (802), the small threaded plate (205) is fixedly connected to the rotating transverse plate (202), the screw rod (802) is connected to the small threaded plate (205) through threads, and the screw rod (802) abuts against the rotating plate (801).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110329050.4A CN113046302A (en) | 2021-03-27 | 2021-03-27 | Stem cell separation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110329050.4A CN113046302A (en) | 2021-03-27 | 2021-03-27 | Stem cell separation method |
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| Publication Number | Publication Date |
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| CN113046302A true CN113046302A (en) | 2021-06-29 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202110329050.4A Withdrawn CN113046302A (en) | 2021-03-27 | 2021-03-27 | Stem cell separation method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115029300A (en) * | 2022-06-20 | 2022-09-09 | 何春兰 | Cell separation method |
-
2021
- 2021-03-27 CN CN202110329050.4A patent/CN113046302A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115029300A (en) * | 2022-06-20 | 2022-09-09 | 何春兰 | Cell separation method |
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Application publication date: 20210629 |