CN219111912U - Animal cell separation equipment - Google Patents
Animal cell separation equipment Download PDFInfo
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- CN219111912U CN219111912U CN202223221440.9U CN202223221440U CN219111912U CN 219111912 U CN219111912 U CN 219111912U CN 202223221440 U CN202223221440 U CN 202223221440U CN 219111912 U CN219111912 U CN 219111912U
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- animal cell
- cap
- circular ring
- cavity
- cell separation
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- 210000004102 animal cell Anatomy 0.000 title claims abstract description 23
- 238000000926 separation method Methods 0.000 title claims abstract description 20
- 238000005192 partition Methods 0.000 claims abstract description 9
- 230000007246 mechanism Effects 0.000 claims description 6
- 210000004027 cell Anatomy 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000008093 supporting effect Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000003889 chemical engineering Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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Abstract
The utility model discloses animal cell separation equipment, which comprises a box body, wherein a cavity is formed in one side of the box body, a partition plate is arranged in the cavity, the upper surface of the partition plate is rotationally connected with a rotary table, the middle part of the upper surface of the rotary table is provided with a fixed disc through a support column, the side wall of the fixed disc is provided with a plurality of U-shaped openings distributed in a circumferential array, and a fixed cap is rotationally connected in each U-shaped opening.
Description
Technical Field
The utility model relates to the technical field of cell separation equipment, in particular to animal cell separation equipment.
Background
Bioengineering is an emerging comprehensive application discipline which starts to rise in the beginning of the 70 s of the 20 th century, and bioengineering based on a system theory, namely the concept of system bioengineering, is generated in the 90 th birth. Bioengineering is generally considered to be an emerging technology which is based on the theory and technology of biology (especially molecular biology, microbiology, genetics, biochemistry and cytology), combines modern engineering technologies such as chemical engineering, machinery, electronic computers and the like, fully utilizes the latest achievements of molecular biology, manipulates genetic materials on site, directionally modifies biology or functions thereof, creates new species with ultra-distant traits in a short period, and cultures the engineering bacteria or engineering cell lines in a large scale through a proper bioreactor so as to produce a large number of useful metabolites or exert unique physiological functions thereof. In the study of animal cells, it is necessary to separate the animal cells, which requires centrifugation using a centrifuge.
The inside a plurality of jacks that are provided with of current animal cell separation equipment, when centrifuging, insert the centrifuging tube in the jack, because the internal diameter of jack is great for the centrifuging tube is put in the jack to one side, when centrifuging, leads to the centrifuging tube to rock easily, and stability is poor, and when fast rotation, the centrifuging tube is rotatory centrifugation in the form of standing approximately, and this kind of centrifugation mode effect is poor, makes centrifugal speed slow.
Disclosure of Invention
The present utility model is directed to an animal cell separation apparatus, which solves the above-mentioned problems of the prior art.
In order to achieve the above purpose, the present utility model provides the following technical solutions:
the utility model provides an animal cell separation equipment, includes the box, box one side is provided with the cavity, be provided with the baffle in the cavity, the upper surface rotation of baffle is connected with the carousel, the upper surface middle part of carousel is provided with the fixed disk through the support column, the lateral wall of fixed disk is provided with a plurality of U type mouths that are circumference array distribution, every all rotate in the U type mouth and be connected with the block cap, the bottom inner chamber of block cap is connected with detachable ring that is used for fixed centrifuging tube, the below of baffle is provided with and is used for driving carousel pivoted actuating mechanism, the opposite side of box is provided with the cabinet door, the top department that the top of box is located the cavity is provided with the opening, the opening part is provided with the pull door.
As a further scheme of the utility model: the driving mechanism comprises a motor arranged at the middle part of the inner wall of the bottom of the cavity, a shaft part of the motor penetrates through the center part of the partition plate and is fixedly connected with the bottom of the turntable, and a shaft part of the motor is rotatably connected with the partition plate through a bearing.
As still further aspects of the utility model: the upper surface of baffle is provided with and is annular spout, the bottom of carousel is provided with four sliders that are circumference array distribution, the bottom and the spout sliding connection of slider, just the radian of slider and the radian phase-match of spout.
As still further aspects of the utility model: both sides of the fixing cap are rotationally connected with both side walls of the U-shaped opening through rotating shafts.
As still further aspects of the utility model: the inner diameter of the circular ring is matched with the diameter of the centrifuge tube cap, a plurality of stoppers distributed in a circumferential array are arranged at the bottom of the circular ring, and the upper surfaces of the stoppers are in contact with the bottom of the centrifuge tube cap.
As still further aspects of the utility model: the outer side wall of the circular ring is provided with external threads, the inner wall of the fixing cap is provided with internal threads, and the circular ring is in threaded connection with the fixing cap.
As still further aspects of the utility model: the middle part of the inner wall of the top end of the fixing cap is provided with a spring, and the bottom of the spring is provided with a supporting plate.
Compared with the prior art, the utility model has the beneficial effects that:
the centrifugal tube centrifugal machine is simple in structure, the centrifugal tube is firmly fixed through the cooperation among the circular ring, the stop block, the fixing cap, the abutting plate and the spring, so that the centrifugal tube cannot shake during centrifugation, stability of the centrifugal tube is improved, the fixing cap is rotationally connected with the U-shaped opening through the rotating shaft, and the fixing cap rotates around the axis of the rotating shaft when the fixing disc rotates, so that the centrifugal tube is thrown away, the centrifugal effect is greatly improved, and quick separation of cells is facilitated.
Drawings
FIG. 1 is a schematic diagram of an animal cell separation apparatus.
Fig. 2 is a front view of fig. 1.
Fig. 3 is a schematic view showing a structure in which a sliding door is opened in an animal cell separation apparatus.
FIG. 4 is a diagram showing the connection between a turntable and a partition plate in an animal cell separating apparatus.
FIG. 5 is a schematic structural view of a stationary plate in an animal cell separation apparatus.
FIG. 6 is a schematic diagram of a fixed cap and fixed centrifuge tube in an animal cell separation apparatus.
Fig. 7 is a front view of fig. 6.
Fig. 8 is an exploded view of fig. 6.
FIG. 9 is a schematic view of the structure of a ring in an animal cell separation apparatus.
In the figure: 1. a case; 2. a cabinet door; 3. a pull door; 4. a cavity; 5. a motor; 6. a partition plate; 7. a turntable; 8. a support column; 9. a fixed plate; 10. a chute; 11. a slide block; 12. a fixing cap; 13. a U-shaped opening; 14. a circular ring; 15. a stop block; 16. a spring; 17. the plate is abutted tightly.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Referring to fig. 1-9, in the embodiment of the utility model, an animal cell separation device comprises a box body 1, a cavity 4 is arranged at one side of the box body 1, a baffle plate 6 is arranged in the cavity 4, a turntable 7 is rotatably connected to the upper surface of the baffle plate 6, a ring-shaped chute 10 is arranged on the upper surface of the baffle plate 6, four sliders 11 distributed in a circumferential array are arranged at the bottom of the turntable 7, the bottom of the sliders 11 is slidably connected with the chute 10, the radian of the sliders 11 is matched with that of the chute 10, a good supporting effect is achieved on the turntable 7 through the cooperation between the sliders 11 and the chute 10, the turntable 7 is more stable when rotating, a fixed disk 9 is arranged at the middle part of the upper surface of the turntable 7 through a supporting column 8, a plurality of U-shaped ports 13 distributed in a circumferential array are arranged at the side wall of the fixed disk 9, a fixed cap 12 is rotatably connected in each U-shaped port 13, the two sides of the fixing cap 12 are rotationally connected with the two side walls of the U-shaped opening 13 through the rotating shaft, so that the fixing cap 12 can rotate by taking the axis of the rotating shaft as the center of a circle, when the fixing disc 9 rotates, the centrifugal tube fixed on the fixing cap 12 can be thrown away, thereby greatly improving the centrifugal effect, being beneficial to quick separation of cells, the inner cavity at the bottom of the fixing cap 12 is connected with a detachable circular ring 14 for fixing the centrifugal tube, the inner diameter of the circular ring 14 is matched with the diameter of the centrifugal tube cap, a plurality of baffle blocks 15 distributed in a circumferential array are arranged at the bottom of the circular ring 14, the upper surface of the baffle blocks 15 is contacted with the bottom of the centrifugal tube cap, the inner diameter of the circular ring 14 is used for inserting the tube body of the centrifugal tube, after the tube body is inserted, the bottom of the centrifugal tube cap is contacted with the upper surface of the baffle blocks 15, so that the centrifugal tube is clamped on the circular ring 14, at the moment, the upper surface of the centrifugal tube cap is flush with the upper surface of the circular ring 14, the outer side wall of the circular ring 14 is provided with external threads, the inner wall of the fixing cap 12 is provided with internal threads, the circular ring 14 is in threaded connection with the fixing cap 12, the circular ring 14 filled with the centrifuge tube is screwed in the inner cavity of the fixing cap 12 through the threaded connection of the circular ring 14 and the fixing cap 12, the circular ring 14 and the fixing cap 12 can be fixed together at the moment, so that the centrifuge tube is driven to fly off when the fixing disc 9 rotates, the middle part of the inner wall of the top end of the fixing cap 12 is provided with a spring 16, the bottom of the spring 16 is provided with a tight propping plate 17, and after the circular ring 14 filled with the centrifuge tube is fixed on the fixing cap 12, the top end of the tube cap of the centrifuge tube is tightly contacted with the bottom of the tight propping plate 17, the centrifuge tube is firmly propped and fixed under the elastic force of the spring 16, and the stability of the centrifuge tube is improved;
the below of baffle 6 is provided with and is used for driving carousel 7 pivoted actuating mechanism, actuating mechanism is including installing in the motor 5 of cavity 4 bottom inner wall middle part, the axial region of motor 5 runs through the central part of baffle 6 and with the bottom fixed connection of carousel 7, and the axial region of motor 5 passes through the bearing and rotates with baffle 6 and be connected, through starter motor 5, make motor 5 drive carousel 7 rotate, thereby make support column 8 follow the rotation of carousel 7 and rotate, thereby make fixed disk 9 follow support column 8 and rotate, the opposite side of box 1 is provided with cabinet door 2, the top department that the top of box 1 is located cavity 4 is provided with the opening, the opening part is provided with pull door 3.
The working principle of the utility model is as follows:
when the centrifugal tube centrifugal machine is used, the drawing door 3 is opened firstly, the circular ring 14 is detached from the fixing cap 12, then the centrifugal tube is placed in the circular ring 14, then the circular ring 14 is fixed on the fixing cap 12, at the moment, under the action of gravity, the centrifugal tube is in a vertical state, then the drawing door 3 is closed, the motor 5 is started, the fixing disc 9 starts to rotate, so that the centrifugal tube rotates along with the rotation of the fixing disc 9, and the centrifugal tube is rotated by taking the axis of the rotating shaft as the center of a circle due to high rotation speed, so that the centrifugal tube is thrown off and is in a horizontal state, and the centrifugal effect is greatly improved.
The foregoing is only a preferred embodiment of the present utility model, but the scope of the present utility model is not limited thereto, and any person skilled in the art, who is within the scope of the present utility model, should make equivalent substitutions or modifications according to the technical scheme of the present utility model and the inventive concept thereof, and should be covered by the scope of the present utility model.
Claims (7)
1. The utility model provides an animal cell separation equipment, its characterized in that, including box (1), box (1) one side is provided with cavity (4), be provided with baffle (6) in cavity (4), the upper surface rotation of baffle (6) is connected with carousel (7), the upper surface middle part of carousel (7) is provided with fixed disk (9) through support column (8), the lateral wall of fixed disk (9) is provided with a plurality of U type mouths (13) that are circumference array distribution, every all rotate in U type mouth (13) and be connected with fixed cap (12), the bottom inner chamber of fixed cap (12) is connected with detachable ring (14) that are used for fixed centrifuging tube, the below of baffle (6) is provided with and is used for driving carousel (7) pivoted actuating mechanism, the opposite side of box (1) is provided with cabinet door (2), the top that the top of box (1) is located the top department of cavity (4) is provided with the opening part, the opening part is provided with pull door (3).
2. An animal cell separating apparatus as claimed in claim 1, wherein the driving mechanism comprises a motor (5) mounted in the middle part of the inner wall of the bottom of the cavity (4), the shaft part of the motor (5) penetrates through the center part of the partition plate (6) and is fixedly connected with the bottom of the turntable (7), and the shaft part of the motor (5) is rotatably connected with the partition plate (6) through a bearing.
3. The animal cell separation equipment according to claim 1, wherein the upper surface of the partition plate (6) is provided with an annular chute (10), the bottom of the turntable (7) is provided with four sliding blocks (11) distributed in a circumferential array, the bottom of the sliding blocks (11) is in sliding connection with the chute (10), and the radian of the sliding blocks (11) is matched with that of the chute (10).
4. The animal cell separating apparatus as claimed in claim 1, wherein both sides of the fixing cap (12) are rotatably connected with both side walls of the U-shaped opening (13) through rotating shafts.
5. An animal cell separation apparatus according to claim 1, wherein the inner diameter of the circular ring (14) is matched with the diameter of the centrifuge tube cap, and a plurality of stoppers (15) distributed in a circumferential array are arranged at the bottom of the circular ring (14), and the upper surface of the stoppers (15) is in contact with the bottom of the centrifuge tube cap.
6. An animal cell separating apparatus as claimed in claim 1, wherein the outer side wall of the circular ring (14) is provided with an external thread, the inner wall of the fixing cap (12) is provided with an internal thread, and the circular ring (14) is in threaded connection with the fixing cap (12).
7. The animal cell separation apparatus according to claim 1, wherein a spring (16) is provided in the middle of the inner wall of the top end of the fixing cap (12), and a pressing plate (17) is provided at the bottom of the spring (16).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202223221440.9U CN219111912U (en) | 2022-12-01 | 2022-12-01 | Animal cell separation equipment |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202223221440.9U CN219111912U (en) | 2022-12-01 | 2022-12-01 | Animal cell separation equipment |
Publications (1)
Publication Number | Publication Date |
---|---|
CN219111912U true CN219111912U (en) | 2023-06-02 |
Family
ID=86529393
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202223221440.9U Active CN219111912U (en) | 2022-12-01 | 2022-12-01 | Animal cell separation equipment |
Country Status (1)
Country | Link |
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CN (1) | CN219111912U (en) |
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2022
- 2022-12-01 CN CN202223221440.9U patent/CN219111912U/en active Active
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Legal Events
Date | Code | Title | Description |
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GR01 | Patent grant | ||
GR01 | Patent grant | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: A device for separating animal cells Granted publication date: 20230602 Pledgee: Zhejiang Jiashan Rural Commercial Bank Co.,Ltd. Chengxi Branch Pledgor: Zhejiang Xirat Biotechnology Co.,Ltd. Registration number: Y2024330000357 |
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PE01 | Entry into force of the registration of the contract for pledge of patent right |