CN220444098U

CN220444098U - Centrifugal machine for separating cells

Info

Publication number: CN220444098U
Application number: CN202320679169.9U
Authority: CN
Inventors: 裴洪; 石亚宽; 陆俊宇
Original assignee: Guangxi Jintian Biotechnology Co ltd
Current assignee: Guangxi Jintian Biotechnology Co ltd
Priority date: 2023-03-31
Filing date: 2023-03-31
Publication date: 2024-02-06
Anticipated expiration: 2033-03-31

Abstract

The utility model discloses a centrifugal machine for separating cells, and belongs to the technical field of centrifugal machines. Comprises a centrifuge shell, a separation assembly and a console; the centrifugal machine shell is internally provided with a mounting groove, the separation assembly is arranged in the mounting groove, and the control console is arranged on the outer side of the centrifugal machine shell and is electrically connected with the separation assembly; the separation assembly comprises a separation motor, a separation rotating shaft, a fixed block, a rubber block and a balance wheel; the separation motor is arranged at the bottom end of the centrifuge shell and is arranged at the center of the mounting groove; the separation rotating shaft is arranged on the separation motor, and the fixed block and the rubber block are sleeved on the separation rotating shaft; the rubber block is arranged at the top end of the fixed block and provided with a centrifugal groove; the balance wheel is arranged at the bottom end of the fixed block and is contacted with the shell of the centrifugal machine. The rubber block has certain elasticity in this application, can provide a buffer force for the test tube when rotating to the balance wheel pair fixed block of setting in the fixed block bottom and the balanced test tube atress when the rubber block rotates improve the stability of cell separation during operation.

Description

Centrifugal machine for separating cells

Technical Field

The utility model relates to the technical field of centrifuges, in particular to a centrifuger for separating cells.

Background

Centrifuges are machines that utilize centrifugal force to separate components of a liquid from solid particles or a mixture of liquid and liquid. The cell centrifuge is a special centrifuge specially used for separating, purifying and transplanting stem cells. The density difference of the separated substances of the cells is small, and the quality of the solution is different in order to prevent the confusion of the separated substances, so that the centrifuge for separating the cells has higher requirements on stability in the running process.

Among the prior art, a high-speed centrifuge for fat mesenchymal stem cells like application number 202222417228.3, including the centrifuge main part, the bonnet, centrifugal rotating shaft, test tube rack and place the slotted hole, the bonnet is supporting to be established at centrifuge main part upper surface, centrifugal rotating shaft is supporting to be equipped with centrifugal rotating shaft, the middle part in the centrifugal rotating shaft outside corresponds to be equipped with test tube rack, the upper surface of test tube rack has been seted up along the circumferencial direction and has been placed the slotted hole, annular groove has been seted up to the position that the circumference side of centrifugal rotating shaft is located the upper surface, this a high-speed centrifuge for fat mesenchymal stem cells, and is rational in infrastructure practical, the simple operation, when the centrifugation is accomplished and is needed taking out the test tube, be convenient for take out the test tube that separation operation accomplished and put into new test tube that waits to separate the operation and carry out separation next time, when need place the separation to the test tube of different pipe diameters size, can be through changing the adjusting ring of different internal diameter sizes, realize placing the test tube of different pipe sizes.

However, in the utility model, the stability of the centrifuge is poor when the centrifuge is used for separating cells, so that shaking is easy to occur in the cell centrifugation process, and the cell separation effect is poor.

Disclosure of Invention

The utility model mainly aims to provide a centrifugal machine for separating cells, and aims to provide a plurality of balance wheels arranged at the bottom end of a separation assembly for balancing stress of test tubes during cell separation, and meanwhile, rubber blocks are used for clamping the test tubes, and the rubber blocks have certain elasticity and can provide buffer force for the test tubes during rotation, so that stability of the separated cells during operation is improved.

To achieve the above object, the present utility model provides a centrifuge for separating cells, comprising:

a centrifuge housing, a separation assembly, and a console; the centrifugal machine shell is internally provided with a mounting groove, the separation assembly is arranged in the mounting groove, and the control console is arranged on the outer side of the centrifugal machine shell and is electrically connected with the separation assembly; the separation assembly comprises a separation motor, a separation rotating shaft, a fixed block, a rubber block and a plurality of balance wheels; the separation motor is arranged at the bottom end of the centrifuge shell and is arranged at the center of the mounting groove; the separation rotating shaft is arranged on the separation motor, and the fixed block and the rubber block are sleeved on the separation rotating shaft; the rubber block is arranged at the top end of the fixed block and provided with a plurality of centrifugal grooves; the balance wheels are arranged at the bottom ends of the fixed blocks and are in contact with the centrifuge shell.

Optionally, the separation assembly further comprises a plurality of test tube installation parts, the plurality of test tube installation parts are in interference fit with the centrifugal groove, and a test tube groove is formed in each test tube installation part.

Optionally, each test tube mounting top is provided with the installation piece, set up on the rubber piece with the mounting groove that the installation piece matches, the mounting groove sets up respectively on corresponding centrifuge groove top.

Optionally, the plurality of mounting blocks are respectively in interference fit with the corresponding mounting grooves.

Optionally, the mount height is greater than the mount channel depth.

Optionally, the test tube grooves formed in the test tube mounting pieces have a plurality of different diameter specifications and are used for adapting to test tubes with a plurality of specifications.

Optionally, a centrifuge top cover is provided on the top of the centrifuge housing, and the centrifuge top cover is matched with the centrifuge housing in shape and size and hinged with the centrifuge housing.

Optionally, one side of centrifuge shell keep away from the control cabinet is provided with first connecting block, the centrifuge top cap outside is provided with the second connecting block, first connecting block passes through the hinge post with the second connecting block and articulates.

Optionally, an observation window is arranged at the top end of the top cover of the centrifugal machine.

Optionally, the centrifuge shell bottom is provided with adjusts lower margin and a plurality of support lower margin.

Compared with the prior art, the application has the following beneficial effects:

the test tube is placed in the centrifugal groove in the rubber block, and the centrifugal groove is in interference fit with the test tube. The separation motor is controlled by the control console, and the separation rotating shaft is driven to drive the fixed block and the rubber block to rotate so as to perform cell separation. The rubber block has certain elasticity, can provide a buffering power for the test tube when rotating to the balance wheel of setting in the fixed block bottom balances the test tube atress when rotating fixed block and rubber block, improves the stability of cell separation during operation.

Drawings

In order to more clearly illustrate the embodiments of the present utility model or the technical solutions of the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the present utility model, and that other drawings may be obtained from the structures shown in these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram of a centrifuge for separating cells according to an embodiment of the present utility model;

FIG. 2 is a cross-sectional view of a centrifuge for separating cells according to the present utility model;

fig. 3 is a schematic view of the test tube mounting member shown in fig. 1.

Reference numerals illustrate:

1. a centrifuge housing; 101. a mounting groove; 2. a separation assembly; 3. a console; 4. separating the motor; 5. separating the rotating shaft; 6. a fixed block; 7. a rubber block; 701. a centrifugal tank; 702. a mounting groove; 8. balance wheel; 9. a test tube mounting member; 901. a test tube groove; 902. a mounting block; 10. a centrifuge top cap; 11. a first connection block; 12. a second connection block; 13. a hinge post; 14. an observation window; 15. adjusting the ground feet; 16. and supporting the ground feet.

The achievement of the objects, functional features and advantages of the present utility model will be further described with reference to the accompanying drawings, in conjunction with the embodiments.

Detailed Description

The following description will be given for the sake of clarity and completeness of the description of the technical solutions according to the embodiments of the present utility model, with reference to the accompanying drawings, wherein it is evident that the described embodiments are only one embodiment of the device according to the present utility model, and not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

It should be noted that all directional indicators (such as up, down, left, right, front, and rear … …) in the embodiments of the present utility model are merely used to explain the relative positional relationship, movement, etc. between the components in a particular posture (as shown in the drawings), and if the particular posture is changed, the directional indicator is changed accordingly.

Furthermore, the description of "first," "second," etc. in this disclosure is for descriptive purposes only and is not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In addition, the technical solutions of the embodiments may be combined with each other, but it is necessary to base that the technical solutions can be realized by those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should be considered to be absent and not within the scope of protection claimed in the present utility model.

Referring to fig. 1 to 3, the present utility model proposes a centrifuge for separating cells.

In one embodiment of the utility model, the centrifuge for separating cells comprises a centrifuge housing 1, a separation assembly 2 and a console 3; a mounting groove 101 is formed in the centrifuge shell 1, the separation assembly 2 is arranged in the mounting groove 101, and the console 3 is arranged outside the centrifuge shell 1 and is electrically connected with the separation assembly 2; the separation assembly 2 comprises a separation motor 4, a separation rotating shaft 5, a fixed block 6, a rubber block 7 and a plurality of balance wheels 8; the separation motor 4 is arranged at the bottom end of the centrifuge shell 1 and is arranged at the center of the mounting groove 101; the separation rotating shaft 5 is arranged on the separation motor 4, and the fixed block 6 and the rubber block 7 are sleeved on the separation rotating shaft 5; the rubber block 7 is arranged at the top end of the fixed block 6 and provided with a plurality of centrifugal grooves 701; the balance wheels 8 are arranged at the bottom ends of the fixed blocks 6 and are in contact with the centrifuge shell 1.

In this application, the mounting groove 101 is a circular groove, and the fixing block 6 and the rubber block 7 are in a cylindrical structure, and rotate in the mounting groove 101 with the separation shaft 5 as a center. A microcomputer is arranged in the control console 3, and parameters such as the rotating speed and the rotating time of the separation motor 4 are controlled through the control console 3. The test tubes are respectively arranged in the centrifugal tanks 701 to separate cells.

The separation assembly 2 further comprises a plurality of test tube mounting pieces 9, and a test tube groove 901 is formed in each test tube mounting piece 9. Test tube setting is in the test tube groove 901, the test tube passes through test tube mounting 9 sets up in the rubber piece 7, a plurality of test tube mounting 9 with centrifugal groove 701 interference fit, will a plurality of test tube mounting 9 press from both sides tightly. The rubber block 7 has certain elasticity, so that the rubber block 7 can give certain buffer when carrying the test tube mounting pieces 9 to rotate, and the stability of cell separation is improved. The test tube grooves 901 formed in the test tube mounting pieces 9 have various different diameter specifications and are used for adapting to test tubes of various specifications.

Each test tube mounting member 9 is provided with a mounting block 902 at the top end, a mounting groove 702 matched with the mounting block 902 is formed in the rubber block 7, and the mounting grooves 702 are respectively formed at the top ends of the corresponding centrifugal grooves 701. The mounting block 902 is arranged on the outer side of the test tube mounting piece 9, and the test tube mounting piece 9 is in interference fit with the mounting groove 702 through the mounting block 902 and is used for limiting the rotation of the test tube mounting piece 9. The mounting 9 has a height greater than the depth of the mounting slot 702. The test tube mounting member 9 arranged in the rubber block 7 is conveniently taken out.

The separation motor 4 is controlled by the control console 3, the separation rotating shaft 5 is driven to drive the fixed block 6 and the rubber block 7 to rotate, a plurality of balance wheels 8 are arranged at the bottom end of the fixed block 6 in a circumferential array mode, and the balance wheels 8 balance the fixed block 6 and the rubber block 7 in a rotating mode, so that stability of cell separation during operation is improved.

The top end of the centrifuge shell 1 is provided with a centrifuge top cover 10, and the shape and the size of the centrifuge top cover 10 are matched with those of the centrifuge shell 1, and the centrifuge top cover is hinged with the centrifuge shell 1 to be in an open state and a closed state. The centrifugal top cover 10 is in an open state, and the test tube installation pieces 9 can be placed in the rubber block 7. The centrifugal top cover 10 is in a closed state, so that external foreign matters can be prevented from falling into the test tube, and substances in the test tube are influenced, and cell separation work is influenced.

The centrifugal machine is characterized in that a first connecting block 11 is arranged on one side, away from the control console, of the centrifugal machine shell 1, a second connecting block 12 is arranged on the outer side of the centrifugal machine top cover 10, a hinge post 13 is arranged on one side, away from the centrifugal machine shell 1, of the first connecting block 11, and one side, away from the centrifugal machine top cover 10, of the second connecting block 12 is connected with the hinge post 13 and is used for hinging the centrifugal machine top cover 10 with the centrifugal machine shell 1.

The top end of the centrifuge top cover 10 is provided with a viewing window 14. The cell separation is performed, the centrifuge top cover 10 is in a closed state, and the state of the test tube can be observed through the observation window 14, so that the problem found in the cell separation is found in time and is suspended.

In addition to the transverse force, the test tube may be subjected to an oblique gravity component due to the oblique placement position of the test tube, and the gravity component may affect the cell separation, for example, causing the cells in the test tube to spill out. In order to reduce the gravitational component, the centrifuge housing bottom end 1 is therefore provided with an adjustment foot 15 and several support feet 16. The supporting ground feet 16 are used as a reference, the adjusting ground feet 15 are adjusted to enable the centrifuge shell 1 to be positioned horizontally, and the test tube is enabled to be vertical, so that the gravity component force is reduced.

When in use, cells to be separated are placed in the test tubes, and test tube mounting pieces 9 with different specifications are selected according to the test tubes with different specifications. The test tube mounting 9 with the test tube placed therein is placed in the centrifuge bowl 701 on the rubber block 7. And the parameters such as the rotating speed and the rotating time of the separation motor 4 are controlled by the control console 3 to perform cell separation work. The test tube mounting piece 9 and the centrifugal groove 701 are in interference fit, and the rubber block 7 has certain elasticity, so that the rubber block 7 can give certain buffering when carrying the test tube mounting pieces 9 to rotate, and the stability of cell separation is improved. The circumference array of a plurality of balance wheels 8 sets up the fixed block 6 bottom, a plurality of balance wheels 8 are right when fixed block 6 and rubber block 7 rotate balances, improves the stability of cell separation during operation.

The foregoing description is only of the preferred embodiments of the present utility model and is not intended to limit the scope of the utility model, and all equivalent structural changes made by the description of the present utility model and the accompanying drawings or direct/indirect application in other related technical fields are included in the scope of the utility model.

Claims

1. A centrifuge for separating cells, comprising a centrifuge housing, a separation assembly, and a console; the centrifugal machine shell is internally provided with a mounting groove, the separation assembly is arranged in the mounting groove, and the control console is arranged on the outer side of the centrifugal machine shell and is electrically connected with the separation assembly; the separation assembly comprises a separation motor, a separation rotating shaft, a fixed block, a rubber block and a plurality of balance wheels; the separation motor is arranged at the bottom end of the centrifuge shell and is arranged at the center of the mounting groove; the separation rotating shaft is arranged on the separation motor, and the fixed block and the rubber block are sleeved on the separation rotating shaft; the rubber block is arranged at the top end of the fixed block and provided with a plurality of centrifugal grooves; the balance wheels are arranged at the bottom ends of the fixed blocks and are in contact with the centrifuge shell.

2. The centrifuge for separating cells of claim 1, wherein the separation assembly further comprises a plurality of tube mounts, the plurality of tube mounts being in interference fit with the centrifuge bowl, each tube mount having a tube bowl formed therein.

3. The centrifuge for separating cells of claim 2, wherein each test tube mounting member is provided with a mounting block at a top end thereof, the rubber block is provided with a mounting groove matched with the mounting block, and the mounting grooves are respectively provided at a top end of the corresponding centrifugal groove.

4. A centrifuge for separating cells as claimed in claim 3 wherein the mounting blocks are each interference fit with a corresponding mounting groove.

5. A centrifuge for separating cells as in claim 3 wherein the mount height is greater than the mount channel depth.

6. A centrifuge for separating cells as defined in claim 2, wherein the plurality of tube mounts have tube slots of a plurality of different diameter sizes for accommodating a plurality of sizes of tubes.

7. The centrifuge for separating cells of claim 1, wherein the centrifuge housing top is provided with a centrifuge top cover that is sized and shaped to mate with the centrifuge housing and is hinged to the centrifuge housing.

8. The centrifuge for separating cells of claim 7, wherein a first connection block is provided on a side of the centrifuge housing remote from the console, a second connection block is provided on an outer side of the centrifuge top cover, and the first connection block is hinged to the second connection block through a hinge post.

9. The centrifuge for separating cells of claim 8, wherein the top of the centrifuge top cap is provided with a viewing window.

10. The centrifuge for separating cells of claim 1, wherein the bottom end of the centrifuge housing is provided with an adjustment foot and a plurality of support feet.