CN216537054U - Centrifugation supernatant pours device - Google Patents

Centrifugation supernatant pours device Download PDF

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CN216537054U
CN216537054U CN202123412791.3U CN202123412791U CN216537054U CN 216537054 U CN216537054 U CN 216537054U CN 202123412791 U CN202123412791 U CN 202123412791U CN 216537054 U CN216537054 U CN 216537054U
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centrifugal
arm
adapter
liquid
waste liquid
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CN202123412791.3U
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方雪
王晨
曾昭红
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Tianjin Bailixin Biological Science & Technology Co ltd
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Tianjin Bailixin Biological Science & Technology Co ltd
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Abstract

Centrifugal supernatant pours device, including centrifugal carousel, adapter and the supplementary device of empting of imbibition, the circumferential edge reason of centrifugal carousel distributes a plurality of adapters, is equipped with between centrifugal carousel and the adapter and pours the mouth, and the supplementary device of empting of imbibition can be followed the linear direction reciprocating motion and made its upset to empty in order to realize promoting the adapter. The centrifugal liquid collecting tray is simple in structure and convenient to operate, the suction mode of the centrifugal liquid collecting tray is changed into pouring, centrifugal precipitation cells are tightly arranged at the bottom of the centrifugal bottle in a centralized mode under the action of centrifugal force, sedimentation is accelerated, after centrifugation is finished, the supernatant liquid is completely discharged under the action of gravity by using the structural characteristics of the mechanical arm and the centrifugal device, and the poured supernatant liquid is collected on the waste liquid collecting tray and is discharged in a centralized mode.

Description

Centrifugation supernatant pours device
Technical Field
The utility model relates to the field of technical waste liquid collection, in particular to a centrifugal supernatant liquid dumping device.
Background
The full-automatic sedimentation type liquid-based cell sheet-making dyeing machine comprises the steps of sample centrifugation, sample transfer, sample sedimentation, dyeing and the like. The supernatant removing process in the current sample centrifugation technology mostly adopts a mode of sucking by a liquid sucking needle, and has the following defects:
1. incomplete supernatant absorption results in insufficient subsequent specimen transfer;
2. stirring the liquid in the process of sucking the supernatant, and sucking and draining the settled cells by sucking the cells back to the liquid again;
3. the pipette needle is cleaned when the supernatant of one specimen is sucked, the operation is complex and time-consuming, then the supernatant of the next specimen can be sucked, and if the cleaning is not in place, cross contamination is easy to cause.
SUMMERY OF THE UTILITY MODEL
The object of the present invention is to solve at least one of the technical drawbacks mentioned.
Therefore, the present invention is directed to a centrifuge supernatant dumping device to solve the above-mentioned problems and overcome the disadvantages of the prior art.
Device is emptyd to centrifugal supernatant, including centrifugal carousel, adapter and the supplementary device of emptying of imbibition, the circumferential edge reason of centrifugal carousel distributes a plurality ofly the adapter, centrifugal carousel with be equipped with the pouring opening between the adapter, the supplementary device of emptying of imbibition can be followed the linear direction reciprocating motion in order to realize the adapter makes its upset emptys.
Preferably, the centrifugal turntable is provided with a start position and a liquid level, the start position is provided with a separation blade, the separation blade is provided with an optical coupler sensor, the optical coupler sensor is connected with the single chip microcomputer, and the liquid level is located at the farthest end of the centrifugal turntable away from the start position.
Preferably, in any one of the above embodiments, a plurality of centrifugal arms are provided on a circumferential edge of the centrifugal turntable, one adapter is provided between adjacent centrifugal arms, a side wall of the adapter is connected to the centrifugal arms through a rotating shaft, and a gap between the centrifugal arms forms the pouring opening.
Preferably, according to any of the above aspects, the width of the pouring opening is not less than the width of the mouth of the specimen bottle placed in the adapter.
Preferably, in any of the above embodiments, the length of the centrifugal arm is not less than the length of the specimen bottle.
Preferably, according to any one of the above schemes, a waste liquid tray is arranged below the pouring opening, and the centrifugal turntable is connected with the waste liquid tray through a rotating shaft.
Preferably, in any one of the above embodiments, the drain hole of the waste liquid tray is disposed below the pouring level.
Preferably, by any one of the above schemes, the waste liquid tray includes a liquid guiding inclined plane and a waste liquid concentration groove, two ends of the waste liquid concentration groove are connected with two ends of the liquid guiding inclined plane to form an integral ring shape, the height of the liquid guiding inclined plane is greater than that of the waste liquid concentration groove, two ends of the liquid guiding inclined plane and two ends of the waste liquid concentration groove form a height difference, and the waste liquid concentration groove is internally provided with the liquid discharge holes.
The liquid suction auxiliary pouring device comprises a mechanical arm, a push rod and a liquid suction gun needle, the push rod and the liquid suction gun needle are arranged below the mechanical arm side by side, and the mechanical arm is connected with the single chip microcomputer.
Preferably, in any of the above schemes, the robot arm moves on the robot arm mounting frame in the horizontal direction, and the push rod and the liquid suction gun needle move on the robot arm in the vertical direction.
By any of the above scheme, be equipped with X on the arm to opto-coupler sensor, Y to opto-coupler sensor, X to separation blade, Y to separation blade, X to hold-in range and Y to the hold-in range, the arm pass through first connecting block with X links to each other just to the drive belt be equipped with X on the first connecting block to the separation blade, the arm mounting bracket pass through the second connecting block with Y links to each other just to the hold-in range be equipped with Y on the second connecting block to the separation blade, X is in to opto-coupler sensor setting X is to one side of hold-in range, Y is in to opto-coupler sensor setting Y is to one side of hold-in range, X to opto-coupler sensor with Y is in to the hold-in range with Y is to same one side of hold-in range.
Compared with the prior art, the utility model has the advantages and beneficial effects that:
the centrifugal liquid collecting tray is simple in structure and convenient to operate, the suction mode of the centrifugal liquid collecting tray is changed into pouring, centrifugal precipitation cells are tightly arranged at the bottom of the centrifugal bottle in a centralized mode under the action of centrifugal force, sedimentation is accelerated, after centrifugation is finished, the supernatant liquid is completely discharged under the action of gravity by using the structural characteristics of the mechanical arm and the centrifugal device, and the poured supernatant liquid is collected on the waste liquid collecting tray and is discharged in a centralized mode.
Drawings
The above and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a view showing the pipette needle in the working position;
FIG. 3 is a state diagram of the push rod in the working position;
FIG. 4 is a diagram illustrating the situation when the pushing rod pushes the adaptor to topple;
FIG. 5 is a side cross-sectional view of the present invention;
FIG. 6 is a schematic view of the structure of the waste liquid tray;
FIG. 7 is a schematic view of a robotic arm;
wherein:
1. a centrifugal turntable; 2. an adapter; 3. a baffle plate; 4. an opto-coupler sensor; 5. a centrifugal arm;
6. a specimen bottle; 7. a waste liquid tray; 8. a push rod; 9. a mechanical arm; 10. a liquid suction gun needle;
11. a drainage slope; 12. a drain hole; 13. a high level surface; 14. a low level; 15. a waste liquid concentration tank;
16. a drain hole joint; 17. a mechanical arm mounting rack; 18. pouring the liquid level; 19. an X-direction synchronous belt;
20. a Y-direction synchronous belt; 21. a baffle plate in the X direction; 22. a Y-direction baffle plate; 23. an X-direction optical coupler sensor;
24. and a Y-direction optical coupler sensor.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the utility model and are not to be construed as limiting the utility model.
In the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
The centrifugal supernatant pouring device shown in figures 1-5 comprises a centrifugal turntable 1, adapters 2 and a liquid suction auxiliary pouring device, wherein a plurality of adapters 2 are distributed on the circumferential edge of the centrifugal turntable 1, a pouring opening is formed between the centrifugal turntable 1 and the adapters 2, and the liquid suction auxiliary pouring device can reciprocate in a linear direction to push the adapters 2 to overturn and pour. Preferably, the adapters 2 are evenly distributed over the circumferential edge of the centrifugal rotor disc 1.
The centrifugal turntable 1 is provided with a start position and a liquid level reversing position 18, the start position is provided with a separation blade 3, the separation blade 3 is provided with an optical coupling sensor 4, and the optical coupling sensor 4 is connected with the single chip microcomputer. The model of the singlechip is STM 32.
The circumferential edge of the centrifugal turntable 1 is provided with a plurality of centrifugal arms 5, an adapter 2 is arranged between every two adjacent centrifugal arms 5, the side wall of the adapter 2 is connected with the centrifugal arms 5 through a rotating shaft, the adapter 2 can rotate on the centrifugal arms 5 through the rotating shaft, a pouring opening is formed in a gap between every two adjacent centrifugal arms 5, the width of the pouring opening is not smaller than the width of a bottle opening of a sample bottle 6 placed in the adapter 2, the adapter 2 is prevented from being turned over, the sample bottle 6 can be blocked by the centrifugal arms 5 and cannot be completely separated from the adapter 2, and an included angle of 95 degrees is formed between the sample bottle 6 and the vertical direction; the length of the centrifugal arm 5 is not less than that of the specimen bottle 6, so that the specimen bottle 6 in the adapter 2 can be fully displaced when the adapter 2 is turned over.
The lower side of the pouring opening is provided with a waste liquid disc 7, the centrifugal turntable 1 is connected with the waste liquid disc 7 through a rotating shaft, and a liquid discharge hole of the waste liquid disc 7 is formed in the lower side of the pouring level.
The liquid suction auxiliary dumping device comprises a mechanical arm 9, a push rod 8 and a liquid suction gun needle 10, the push rod 8 and the liquid suction gun needle 10 are arranged below the mechanical arm 9, the push rod 8 and the liquid suction gun needle 10 are arranged side by side, and the mechanical arm 9 is connected with the single chip microcomputer.
The mechanical arm 9 moves on the mechanical arm mounting frame 17 along the horizontal direction, and the push rod 8 and the liquid suction gun needle 10 move on the mechanical arm 9 along the vertical direction.
As shown in fig. 7, be equipped with on arm 9 that X is to opto-coupler sensor 23, Y to opto-coupler sensor 24, X to separation blade 21, Y to separation blade 22, X to hold-in range 19 and Y to hold-in range 20, arm 9 through first connecting block and X to drive belt 19 link to each other just be equipped with X on the first connecting block to separation blade 21, arm mounting bracket 17 through the second connecting block with Y to hold-in range 20 link to each other just be equipped with Y on the second connecting block to separation blade 22, X to opto-coupler sensor 23 sets up in one side of X to hold-in range 19, and Y to opto-coupler sensor 24 sets up in one side of Y to hold-in range 20, and X is to opto-coupler sensor 23 and Y to opto-coupler sensor 24 setting in X to hold-in range 19 and Y to the same one side of hold-in range 20.
As shown in FIG. 6, the waste liquid tray 7 comprises a liquid guiding inclined plane 11 and a liquid discharging hole 12, wherein a high level surface 13 of the liquid guiding inclined plane 11 forms a ring shape to a low level surface 14 of the liquid guiding inclined plane 11, and a processing inclined plane with a height difference has good flow guiding for the poured waste liquid.
The liquid discharge hole 12 is located at the lower level 14 of the liquid guide inclined plane 11, a waste liquid concentration groove 15 is arranged at the liquid discharge hole 12, and two ends of the waste liquid concentration groove 15 are connected with two ends of the liquid guide inclined plane 11 to form an integral ring shape.
The height of the liquid guiding inclined plane 11 is greater than that of the waste liquid collecting groove 15, height difference is formed between two ends of the liquid guiding inclined plane 11 and two ends of the waste liquid collecting groove 15, and the sudden high fall enables waste liquid washed and stored in the waste liquid collecting groove 15 to obtain larger potential energy, so that residual massive tissues and mucus can be easily washed to the liquid discharge hole 12 to be concentrated.
The liquid discharge hole 12 is connected with a waste liquid bottle, the pressure in the liquid discharge hole 12 is-15 Kpa, when the waste liquid flows to the position nearby the liquid discharge hole 12, the waste liquid can be sucked away by negative pressure, and the effects that most of the waste liquid is discharged completely and large-area residue is avoided are achieved.
The device is made of engineering plastics, and the corrosion phenomenon caused by the acidity and alkalinity of the waste liquid can not occur by using the engineering plastics, so that the service life of parts is prolonged.
The waste liquid flows into the waste liquid collecting groove 15 from the high level surface 13 to the low level surface 14 of the liquid guide inclined surface, the height difference between the low level surface 14 and the waste liquid collecting groove 15 increases the kinetic energy of liquid flowing, and then the waste liquid is sucked away by negative pressure when flowing to the position near the liquid discharge hole, so that most of the waste liquid is discharged completely and cannot be left in a large area.
The working process is as follows:
the sample rotates through the centrifugal disc, a signal is triggered by the baffle plate 3 through the optical coupling sensor 4, the sample is fed back to the single chip microcomputer to record that the position of the adapter 2 farthest from the baffle plate 3 is the inverted liquid level 18, the number of the adapters 2 is known, the circumference is equally divided by 360 degrees according to the number of the adapters 2, the number of the steps of the operation of the centrifugal turntable motor is input into the singlechip of the mechanical arm, the rotation of each adapter 2 can be controlled by the singlechip to enable the adapters 2 to sequentially reach the position of the liquid level 18, the operation steps of the centrifugal turntable motor are input into the singlechip of the mechanical arm, and the rotation of the centrifugal turntable motor can be controlled by the singlechip to enable the adapters 2 to sequentially reach the position of the liquid level 18. For example: and the 12 adapters 2 are equally divided into 360 degrees, the interval angle between each adapter 2 is 30 degrees, and the centrifugal turntable motor is controlled to rotate by the single chip microcomputer according to the interval angle so that each adapter 2 sequentially reaches the liquid pouring level 18 according to clockwise/anticlockwise rotation. As shown in fig. 2, the pipetting needle 10 on the mechanical arm 9 is moved to the position, the X-direction optical coupler sensor 23 and the Y-direction optical coupler sensor are respectively arranged in the XY direction on the mechanical arm, when the X-direction blocking piece 21 and the Y-direction blocking piece 22 on the mechanical arm 9 block the optical coupler in the direction, the mechanical arm 9 stops, the position is defined as 0 point in the X or Y direction, then when the mechanical arm 9 moves in the X or Y direction, the coordinate value of the pipetting needle on the mechanical arm 9 is correspondingly changed according to the reference of the 0 point, when the pipetting needle 10 on the mechanical arm 9 aims at the center of the circle of the sample bottle 6 in the adapter, the coordinate value displayed by the program is checked, and the value is recorded in the program. The X, Y direction coordinate of the mechanical arm 9 is recorded into the mechanical arm singlechip by the coordinate of the point (burned into the singlechip circuit board by the burner). The robotic arm 9 will automatically return to this point each time a sample needs to be taken, and then lower the height of the pipetting needle 10 to take the sample.
As shown in figure 3, because the relative distance between the push rod 8 and the liquid suction gun needle 10 is fixed, the calculated locating coordinate of the push rod 8 is added on the basis of the original point coordinate and recorded in the singlechip, the coordinate point can enable the push rod 8 to be located at the right side position of the current sample bottle 6, then the singlechip drives the mechanical arm 9 to move to the position and then the push rod 8 sinks to the height of the adapter 2, at the moment, the singlechip controls the mechanical arm 9 to move horizontally, the push rod 8 moves leftwards and exerts an acting force on the bottle body of the sample bottle 6 as shown in figures 4 and 5, due to the structural characteristics of the adapter 2, the sample bottle 6 slowly inclines to the horizontal and continuously inclines downwards under the acting force of the push rod 8, and because the diameter of the bottle mouth is larger than the included angle gap of two adjacent centrifugal arms 5 on the edge of the centrifugal disc 7, the sample bottle 6 can not be blocked by the centrifugal arms 5 out of the adapter 2, and forms an angle of about 95 deg. at the most with the vertical direction, which allows the supernatant liquid inside the specimen bottle 6 to flow into the waste liquid tray 7 along the side wall of the specimen bottle 6 and finally to be discharged.
After pouring, the mechanical arm 9 slowly returns to the position before touching the sample bottle 6, and the adapter 2 and the sample bottle 6 can rotate to reset under the action of gravity and return to the position with the bottle mouth facing upwards;
the centrifugal turntable 1 is controlled by a centrifugal motor to continue to rotate for a certain number of steps, so that the next adapter 2 reaches the pouring liquid level 18, and the actions are continuously repeated to finish pouring the waste liquid until the end.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the utility model. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
It will be understood by those skilled in the art that the utility model includes any combination of the elements of the foregoing description and the detailed description as well as those illustrated in the drawings, which are meant to be space-limited and not intended to constitute a part of the specification in any way whatsoever, the combination being such that the description is not intended to be exhaustive. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made in the above embodiments by those of ordinary skill in the art without departing from the principle and spirit of the present invention. The scope of the utility model is defined by the appended claims and equivalents thereof.

Claims (10)

1. Centrifugation supernatant pours device, its characterized in that: including supplementary device of empting of centrifugal carousel, adapter and imbibition, the circumferential edge reason of centrifugal carousel distributes a plurality ofly the adapter, centrifugal carousel with be equipped with the pouring opening between the adapter, the supplementary device of empting of imbibition can be followed linear direction reciprocating motion and in order to realize the promotion the adapter makes its upset empty.
2. The centrifuge supernatant pouring device of claim 1, wherein: the centrifugal turntable is provided with a start position and a liquid level, the start position is provided with a separation blade, the separation blade is provided with an optical coupling sensor, the optical coupling sensor is connected with a single chip microcomputer, and the liquid level is located at the farthest end of the centrifugal turntable away from the start position.
3. The centrifuge supernatant pouring device of claim 2, wherein: the circumference edge of centrifugal carousel is equipped with a plurality of centrifugal arms on, be equipped with one between the adjacent centrifugal arm the adapter, the lateral wall of adapter with the centrifugal arm links to each other through the pivot, clearance between the centrifugal arm forms the pouring opening.
4. A centrifugal supernatant pouring device as defined in claim 3 wherein: the width of the pouring opening is not less than the width of the bottle mouth of the specimen bottle placed in the adapter.
5. The centrifuge supernatant pouring device of claim 4, wherein: the length of the centrifugal arm is not less than the length of the specimen bottle.
6. The centrifuge supernatant pouring device of claim 4, wherein: and a waste liquid disc is arranged below the pouring opening, and the centrifugal turntable is connected with the waste liquid disc through a rotating shaft.
7. The centrifuge supernatant pouring device of claim 6, wherein: and the liquid discharge hole of the waste liquid tray is arranged below the liquid pouring level.
8. The centrifuge supernatant pouring device of claim 7, wherein: the waste liquid disc comprises a liquid guide inclined plane and a waste liquid concentration groove, two ends of the waste liquid concentration groove are connected with two ends of the liquid guide inclined plane to form a whole ring, the height of the liquid guide inclined plane is larger than that of the waste liquid concentration groove, two ends of the liquid guide inclined plane and two ends of the waste liquid concentration groove form a height difference, and the waste liquid concentration groove is internally provided with liquid discharge holes.
9. A centrifugal supernatant pouring device according to any one of claims 1 to 8, wherein: the supplementary device of empting of imbibition includes arm, arm mounting bracket, push rod and imbibition rifle needle, the arm is installed on the arm mounting bracket, the below of arm is equipped with push rod and imbibition rifle needle, the push rod with the imbibition rifle needle sets up side by side, the arm links to each other with the singlechip.
10. The centrifuge supernatant pouring device of claim 9, wherein: be equipped with X on the arm to opto-coupler sensor, Y to opto-coupler sensor, X to separation blade, Y to separation blade, X to hold-in range and Y to the hold-in range, the arm pass through first connecting block with X links to each other just to the drive belt be equipped with X on the first connecting block to the separation blade, the arm mounting bracket pass through the second connecting block with Y links to each other just to the hold-in range Y is equipped with Y on the second connecting block to the separation blade, X is in to opto-coupler sensor setting X is to one side of hold-in range, Y is in to opto-coupler sensor setting Y is to one side of hold-in range, X is to opto-coupler sensor with Y is in to opto-coupler sensor setting X to the hold-in range with Y is to same one side of hold-in range.
CN202123412791.3U 2021-12-31 2021-12-31 Centrifugation supernatant pours device Active CN216537054U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202123412791.3U CN216537054U (en) 2021-12-31 2021-12-31 Centrifugation supernatant pours device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202123412791.3U CN216537054U (en) 2021-12-31 2021-12-31 Centrifugation supernatant pours device

Publications (1)

Publication Number Publication Date
CN216537054U true CN216537054U (en) 2022-05-17

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202123412791.3U Active CN216537054U (en) 2021-12-31 2021-12-31 Centrifugation supernatant pours device

Country Status (1)

Country Link
CN (1) CN216537054U (en)

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