CN108865772A - A kind of centrifuge tube - Google Patents
A kind of centrifuge tube Download PDFInfo
- Publication number
- CN108865772A CN108865772A CN201710327018.6A CN201710327018A CN108865772A CN 108865772 A CN108865772 A CN 108865772A CN 201710327018 A CN201710327018 A CN 201710327018A CN 108865772 A CN108865772 A CN 108865772A
- Authority
- CN
- China
- Prior art keywords
- centrifuge tube
- lower chambers
- blood
- chamber
- middle chamber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/06—Tubular
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
Abstract
The present invention relates to a kind of centrifuge tube, especially a kind of centrifuge tube for the separation of human peripheral blood ingredient.The centrifuge tube includes pipe lid, elastic sealed body, centrifuge tube tube body, upper chamber, middle chamber, lower chambers and lower chambers bottom surface.The present invention is good to different cell layered effects, and convenient for extracting cell, and structure is simple, is more convenient for manufacturing.
Description
Technical field
The present invention is about a kind of centrifuge tube, especially a kind of centrifuge tube for human peripheral blood.
Background technique
In biology, science of heredity and medical science, the most basic operation of when separation of substance.Currently, in medical domain,
It is increasingly gradually deep to the research of stem cell.In order to obtain specific cell, often cell is separated.For example, obtaining adult
Main three kinds of methods of stem cell：Adult stem cell is obtained by marrow, adult stem cell is obtained by Cord blood and by outer
All blood obtains adult stem cell.In these methods, each ingredient of blood is separated by centrifugation first（Red blood cell is white thin
Born of the same parents, blood platelet and blood plasma）, then separate stem cell（Monocyte）.Conventional operating process is to remove centrifuge tube pipe lid, is added
Enter the peripheral blood extracted, covers centrifuge tube pipe lid, centrifuge tube is then placed in a centrifuge centrifugation.In general, red blood cell is in blood
Specific gravity in liquid all the components is maximum, therefore is often being centrifuged bottom of the tube after being centrifuged, and red blood cell accounts for people's whole blood 47% or so.Position
What it is in red blood cell upper layer is leucocyte and blood platelet, accounts for 1% of whole blood or so, and wherein further include monocyte（Peripheral blood
Stem cell）.Blood plasma and fibrin etc. are located at the top of leucocyte and blood platelet.The centrifuge tube being centrifuged is taken out from centrifuge
Afterwards, it need to be again turned on plug, and be layered according to each material composition because of different specific weight to draw required target.
In such operating process, exist open and close plug the step of, can make the foreign matter in air enter from
In heart pipe, there is a possibility that pollution sample.In addition, it is one cylindrical that conventional centrifuge tube, which is upper middle part, bottom is class cone
Shape.Using this centrifuge tube, if the target after centrifugation is to be deposited in bottom, more convenient taking-up.If but required target be located at from
In the middle part of heart pipe or higher position, the biggish diameter of centrifuge tube will be unfavorable for being precisely separated required target.
In consideration of it, it is necessary to provide a kind of centrifuge tube, to solve the above problems.
Summary of the invention
The object of the present invention is to provide a kind of centrifuge tubes, can conveniently and efficiently separate, extract each ingredient in blood.
To realize that above-mentioned target, the present invention provide a kind of centrifuge tube, which includes pipe lid, elastic sealed body, centrifugation
Pipe tube body, upper chamber, middle chamber, lower chambers and lower chambers bottom surface.Wherein, the elastic sealed body is by rubber, silicone resin, PVC,
Polyethylene, one or more materials are made in polypropylene.The lower chambers bottom surface has elasticity, and by rubber, silicone resin, PVC gathers
Ethylene, one or more materials are made in polypropylene.The upper chamber possesses diameter same as lower chambers, the middle chamber
Diameter is the half of upper chamber.The lower chambers account for centrifuge tube total volume about 40%, and the middle chamber accounts for centrifuge tube total volume about
10%。
The invention has the advantages that.
Peripheral blood has been extracted in syringe, elastic sealed body can be inserted directly into and enter centrifuge tube, peripheral blood is discharged, then takes out
Syringe out.Whole process centrifuge tube internal objects do not contact outside air, can effectively avoid outside air to sample in centrifuge tube
This potential pollution.
In addition, because middle chamber diameter is only the half of upper and lower chamber, positioned at middle chamber at layering under equal volume,
There to be bigger height, convenient for extracting the ingredient for being located at middle chamber.
Detailed description of the invention
Fig. 1 is the sectional view of centrifuge tube of the invention.
Fig. 2 is 1 schematic diagram of the embodiment of the present invention.
In figure, 1, centrifuge tube pipe lid；2, elastic sealed body；3, upper chamber；4, centrifuge tube tube body；5, middle chamber；6, cavity of resorption
Room；7, lower chambers bottom surface.
Specific embodiment
Below by way of specific embodiment, the present invention is further explained.
A kind of centrifuge tube, the centrifuge tube include pipe lid 1, elastic sealed body 2, centrifuge tube tube body 4, upper chamber 3, middle chamber 5,
Lower chambers 6 and lower chambers bottom surface 7.Wherein, the elastic sealed body 2 is by rubber, silicone resin, PVC, polyethylene, one in polypropylene
Kind or multiple material are made.The lower chambers bottom surface 7 has elasticity, by rubber, silicone resin, and PVC, polyethylene, one in polypropylene
Kind or multiple material are made.The upper chamber 3 possesses diameter same as lower chambers 6, and the diameter of the middle chamber 5 is upper chamber
3 half.The lower chambers 6 account for centrifuge tube total volume about 40%, and the middle chamber 5 accounts for centrifuge tube total volume about 10%.
Embodiment 1：Suitable Percoll solution is drawn using syringe, insertion elastic sealed body 2 enters in centrifuge tube,
Percoll solution is discharged.Then the syringe insertion elastic sealed body 2 for having extracted peripheral blood is entered in centrifuge tube, injection is outer
All blood is extracted syringe out, is centrifuged in telling centrifuge.After centrifugation, mixed liquor will be layered.Such as Fig. 2, distinguish from bottom to top
It is red blood cell and granulocyte part, the part rich in lymphocyte, part and dead cell part rich in monocyte.
Claims (7)
1. a kind of centrifuge tube, which is characterized in that the centrifuge tube includes pipe lid（1）, elastic sealed body（2）, centrifuge tube tube body（4）,
Upper chamber（3）, middle chamber（5）, lower chambers（6）With lower chambers bottom surface（7）.
2. centrifuge tube according to claim 1, it is characterised in that：The elastic sealed body（2）By rubber, silicone resin,
PVC, polyethylene, one or more materials are made in polypropylene.
3. centrifuge tube according to claim 1, it is characterised in that：The lower chambers bottom surface（7）With elasticity, by rubber,
Silicone resin, PVC, polyethylene, one or more materials are made in polypropylene.
4. centrifuge tube according to claim 1, it is characterised in that：The middle chamber（5）Diameter be upper chamber（3）One
Half.
5. centrifuge tube according to claim 1, it is characterised in that：The upper chamber（3）Possess and lower chambers（6）Similarly
Diameter.
6. centrifuge tube according to claim 1, it is characterised in that：The lower chambers（6）Account for centrifuge tube total volume about 40%.
7. centrifuge tube according to claim 1, it is characterised in that：The middle chamber（5）Account for centrifuge tube total volume about 10%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710327018.6A CN108865772A (en) | 2017-05-10 | 2017-05-10 | A kind of centrifuge tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710327018.6A CN108865772A (en) | 2017-05-10 | 2017-05-10 | A kind of centrifuge tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108865772A true CN108865772A (en) | 2018-11-23 |
Family
ID=64319242
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710327018.6A Pending CN108865772A (en) | 2017-05-10 | 2017-05-10 | A kind of centrifuge tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108865772A (en) |
-
2017
- 2017-05-10 CN CN201710327018.6A patent/CN108865772A/en active Pending
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20181123 |
|
| WD01 | Invention patent application deemed withdrawn after publication |