KR101569172B1

KR101569172B1 - Component separating device

Info

Publication number: KR101569172B1
Application number: KR1020150073816A
Authority: KR
Inventors: 김준우
Original assignee: 김준우
Priority date: 2015-05-27
Filing date: 2015-05-27
Publication date: 2015-11-13

Abstract

The present invention relates to an ingredient separation apparatus. The ingredient separation apparatus can simplify the process compared with a conventional technology, ensure the purity of a target ingredient such as a stem cell or separated blood, and prevent air contamination during an ingredient separation process. The apparatus includes: a body which has a flow pipe formed on an end and a space on the inside; a moving pipe body which moves forward and backward in the body, has an outer plunger installed on an end, and has a space on the inside connected to the space of the body; and a rotating rod which is coupled to a moving pipe body screw unit formed on the moving pipe body by a screw and has an inner plunger formed on a distal end capable of being inserted to and being drawn out from an outer plunger hole formed on the outer plunger, wherein the moving pipe body moves against the body while a moving pipe body extending unit formed on the other end of the moving pipe body comes in contact with the surface of the inner wall of the body.

Description

Component separating device

More particularly, the present invention relates to a device for separating a component, which is capable of ensuring purity of an objective component such as separated blood or stem cells while simplifying a process, And to a component separator having the same.

There are various treatments or procedures using stem cells. It can be used for cosmetic and cosmetic procedures, and can be used for hair loss treatment, chronic intractable disease and pain treatment. Its application range is considerably wide, and its application range is expected to expand further in the future. Stem cells are cells that have the ability to self-renew and differentiate into other cells.

Stem cells can be divided into embryonic stem cells and adult stem cells. The other stem cells include cord blood stem cells, umbilical cord stem cells, and villus (placenta) stem cells, and the stem cell group includes bone marrow-stem cell group derived stem cells and adipose derived stem cells (ADSC). These cells are mainly hematopoietic stem cells (HSC) and mesenchymal stem cells (MSC). The method of isolating existing stem cells is based on the method of extracting from the laboratory, and the extraction process is carried out. However, the extraction method is difficult, difficult to learn, takes a lot of time, There is a disadvantage that it is possible to cause pollution and contamination by the apparatus and that there is a considerable difference in efficiency depending on the person who extracts. For this reason, there is a need for easier and simpler methods of preventing contamination.

Platelets, which are one of the constituents of blood, contain many kinds of growth factors. Therapy using these growth factors has a good effect on skin diseases and wound healing. Growth factors in these platelets have been used in the form of PRP (Platelet Rich Plasma). By centrifugation, most of the platelets and white blood cells are located in the buffy coat layer, which is formed at the interface between blood cells and plasma. Plasma (PPP) (Platelet Poor Plasma) is also used for various medical purposes.

Therefore, for various medical purposes, it is necessary to develop a more convenient and advanced method of extracting each constituent element after separating body fluids using the difference in specific gravity between cells.

Patent Registration No. 10-1179548

It is an object of the present invention, which is devised to solve the above-described problems, to provide a method and apparatus that can simplify a process and ensure purity of an objective component such as separated blood or stem cells, And to provide a component separating device that can be used.

According to an aspect of the present invention, there is provided a portable terminal comprising: a body having a flow tube formed at one end thereof and having a space therein; A movable conduit body having a back and forth inside the body, an outer plunger at one end, and a space communicating with the space of the body; And a rotary rod having an inner plunger threadably coupled to the moving pipe threaded portion formed on the moving pipe body and being insertable into an outer plunger hole formed in the outer plunger at one end thereof and a movable pipe body expanding portion formed at the other end of the moving pipe body, And the moving pipe moves with respect to the main body while being in contact with the inner wall surface of the main body.

And the upper surface of the outer plunger formed on the outer plunger toward the flow tube is formed to be inclined toward the inner plunger.

Further, the rotary rod is provided with an offset having no thread.

A stopper is formed on an inner wall of the main body so as to limit the movement of the moving pipe.

Further, an inner filter is provided in the outer plunger hole.

Through the present invention, the time and cost required for blood component separation can be reduced. Particularly, the separation of PRP and PPP can be minimized or prevented due to the separation of the upper part in the order of light PPP and PRP, so that the purity of separated PRP and PPP is also assured.

In addition, since blood is moved away from the outside air during blood collection to blood separation, contamination by air can be prevented. Therefore, it is possible to prevent the occurrence of infection or complications of pathogenic bacteria which may be expected in the air contamination of the blood during the blood separation process.

Also, stem cells can be isolated through the present invention. In order to isolate very small amount compared to the undiluted solution, high-purity stem cells can be easily extracted by adopting a special type of working member which enables easy extraction of stem cells. Therefore, treatment cost can be drastically reduced when treating with stem cells.

According to the present invention, it is possible to separate various target components in addition to stem cells and blood components. In particular, it is also possible to separate the liquid equivalent in the direction opposite to that formed in the figure.

1 is a perspective view of a component separator according to a first embodiment of the present invention.
2 is an exploded perspective view of the component separating apparatus of FIG.
3 is a cross-sectional view of the component separator of FIG.
FIG. 4 is a cross-sectional view of the component separator of FIG. 1 in a state where components to be separated are layered and packed.
5 is a cross-sectional view of the state in which the inner plunger is retracted in the state of FIG. 4 relative to the outer plunger, and the object liquid flows into the moving body through the outer plunger hole.
Fig. 6 is a cross-sectional view of the state in which the movable conduit is raised to discharge the separated liquid in the state of Fig. 5;
7 is a cross-sectional view of a component separator according to Embodiment 2 of the present invention.

Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings. In the drawings, the same reference numerals are used to designate the same or similar components, and the same reference numerals will be used to designate the same or similar components. Detailed descriptions of known functions and configurations are omitted.

In the present invention, the objective liquid refers to a liquid containing a target component or a target component in a liquid state. The separation liquid refers to a liquid component containing a component to be separated or a component to be separated, which the user desires to separately discharge.

1 to 6, reference numeral 100 denotes a component separation apparatus according to Embodiment 1 of the present invention. The component separating apparatus 101 basically comprises a main body 102 having a flow tube 104 formed at one end thereof and having a space therein and an outer plunger 118 disposed at one end of the main body 102, A movable pipe 112 having a space communicated with the space of the main body 102 and a movable pipe threaded portion 124 formed on the movable pipe 112, And an inner plunger 118 insertable into an outer plunger hole 122 formed in the outer cylinder 118.

The main body 102 is preferably made of a transparent material that can see the inside, and may be made of plastic, glass, or the like. The flow tube 104 may be connected to another syringe or the like, or a stopper (not shown) may be coupled.

The movable pipe 112, which can be moved inside the main body 102, is also preferably made of a transparent material that can see the inside, and can be made of plastic, glass, or the like.

An external plunger 118 is installed at an upper end of the moving pipe 112. The outer plunger 118 abuts the inner wall surface of the main body 102, and the outer plunger hole 122 is formed at the center. In particular, the outer plunger upper surface 120 of the outer plunger 118 facing the flow tube 104 is formed to be sloped toward the outer plunger hole 122. The flow pipe peripheral portion 106 formed around the flow pipe 104 is also inclined to correspond to the upper face 120 of the outer plunger. As a result, the liquid located on the upper side of the outer plunger 118 can easily flow into the outer plunger hole 122.

An outer plunger fixing portion 114 is formed on the moving pipe 112 to support the outer plunger 118. The outer plunger fixing portion 114 can have various shapes. In the first embodiment, the outer plunger fixing part 114 includes a supporting plate for supporting the lower surface of the outer plunger 118, and a supporting loop inserted and supported inside the outer plunger 118.

A stopper 110 is formed on the inner wall of the main body 102 so as to limit the movement of the moving pipe 112. The stopper 110 may be in contact with the movable conduit extension 116 of the moving pipe 112 or the outer plunger fixing part 114 and may be in contact with other positions.

The moving pipe body extension portion 116 is formed at an end of the moving pipe body 112 opposite to the outer plunger 118. The moving pipe body 112 is moved while the moving pipe body extending portion 116 is in contact with the inner wall of the main body 102. As a result, when the component separating apparatus 100 is installed in a centrifugal separator (not shown) or the like, the moving pipe 112 can maintain its posture without vibrating with respect to the main body 102.

In addition, it is preferable that the movable conduit extension portion 116 is formed in a cross shape or the like so that the user can grasp it by using a hand or a tool. That is, it is possible to force the moving pipe body 112 to move forward and backward by holding the moving pipe body extending portion 116.

The rotating rod 126 inserted into the moving pipe 112 has an inner plunger 128 installed at one end thereof and inserted into the outer plunger hole 122 of the outer plunger 118. A thread formed on the outer circumferential surface of the rotary rod 126 may be formed on the entirety of the rotary rod 126, or may be formed only on a part of the rotary rod 126, and the rest may have a threadless offset. 5, in the state where the inner plunger 128 is inserted into the outer plunger 118 as shown in FIGS. 3 and 4, ) Must be arranged to be able to retreat.

In addition, a moving pipe thread portion 124 corresponding to the thread of the rotating rod 126 is formed in the moving pipe body 112. The moving tube 112 and the rotating rod 126 move integrally with each other when the threaded portion of the rotating rod 126 and the moving tube threaded portion 124 are engaged with each other. 4 to the state shown in FIG. 4, the outer plunger 118 and the inner plunger 128 can be moved by the same amount of displacement.

A rod knob 130 is formed at the other end of the rotating rod 126 opposite to the inner plunger 128. The rod handle 130 rotates the rotary rod 126 to move the rotary rod 126 back and forth. In particular, when the rotary rod 126 has a threadless offset, the rod handle 126 130 to rotate the rotary rod 126 back and forth without rotation.

The component separating apparatus 100 according to the first embodiment of the present invention is basically configured as described above. Hereinafter, the component separation process by the component separation apparatus 100 will be described.

The object liquid in which the object liquid and the separation liquid are mixed flows into the distribution pipe 104 of the component separation apparatus 100. [ Here, the target liquid is blood, liquid mixed with stem cells, and the like.

When the blood is used as the target liquid, it can be separated from blood cells, PPP and PRP. Platelet rich plasma (PRP) refers to plasma including a buffy coat layer, and PPP refers to plasma that does not include a buffy coat layer. Therefore, when the blood is charged into the component separator 100 and the blood is separated by a centrifuge or the like, the blood is layered from the bottom to the bottom of blood, the buffy coat and the plasma. Therefore, it is possible to separate by selecting the desired solution from blood cells, PPP, and PRP, and leaving a part of the solution in the component separator 100 and discharging the rest.

In addition, the use of a liquid containing adipose-derived stem cells as a target liquid will be described. First, to extract fat-derived stem cells, fat is obtained from the human body by inhalation or the like. Then, the obtained fat is subjected to a decanting process or a centrifugation process to remove water and obtain pure fat. Thereafter, a process (incubation process) is carried out in which a solution of collagenase mixed with saline and collagenase is mixed with pure fat to allow the stem cells existing between the adipocytes to be sufficiently liberated, Is charged into the component separating apparatus (100). Alternatively, an incubation process for mixing the pure fat and the collagenase solution and then directly feeding the solution into the component separating apparatus 100 to free stem cells present in the adipocytes may be performed in the component separating apparatus 100 have.

When the liquid containing the stem cells is layered, the stem cell liquid has a specific gravity which is high and the supernatant is arranged on the upper side. Strictly speaking, the mass of cells present in the form of pellet at the lowermost end is a stem cell fraction, and the state in which this stem cell fraction is suspended in a small amount of the supernatant 6 is called a stem cell fluid For convenience, both stem cell fractions and stem cell fluids are referred to as stem cell fluids. When the supernatant is largely discharged using the component separator 100, the lower part of the stem cell solution can be separated. Particularly, the washing solution is further supplied to the component separating apparatus 100, and the process of layer separation of the supernatant by the centrifugal separator is repeated three to four times to finally obtain a high-purity stem cell solution.

As with adipose-derived stem cells, bone marrow-derived stem cells can be isolated. In the case of bone marrow-derived stem cells, bone marrow is first extracted using a special bone marrow extraction needle at the iliac bone and other sites. Since the bone marrow extract has a similar pattern to blood, it can be extracted by the same method as the blood component separation method mentioned above. In addition, by using the above-described method, lymphocytes, immune cells, cord blood stem cells and the like can be separated using the specific gravity difference of the cells. In other words, allogeneic cells or xenogeneic cells can be separated using the above method using the specific gravity difference of the cells.

A plurality of target liquids are charged into a space formed between the flow channel 104 and the outer plunger 118 and the inner plunger 128 in the body 102 through the flow channel 104 as shown in FIG. After that, layer separation is performed in a centrifuge (not shown) or the like. At this time, the flow pipe 104 is closed by a cap (not shown).

The object liquid is charged into the component separating apparatus 100 by an amount allowed by the stopper 110. When the amount is smaller than the allowable amount, the stopper 110 restricts the position where the moving pipe 112 retracts inside the centrifuge.

When the layer separation is completed, the object liquid 1 and the separation liquid 2 are separated as shown in FIG. Here, one or more layers may be included in each of the objective liquid (1) and the separation liquid (2). For the sake of convenience, it is assumed that the objective liquid (1) is disposed at the lower portion and the separation liquid (2) is disposed at the upper portion.

5, by moving the outer plunger 118 backward by operating the rotating rod 126, the movable plunger 112 is rotated through the outer plunger hole 122 of the outer plunger 118, The object liquid 1 flows into the upper space of the inner plunger 128 from the inside thereof.

In this state, when the object liquid 1 flows into the upper space of the inner plunger 128 inside the moving pipe 112 by pushing up the moving pipe 112, (2) can be discharged. The rotating rod 126 is not displaced with respect to the moving pipe 112 because the threads of the moving pipe thread portion 124 and the rotating rod 126 are engaged.

Therefore, the object liquid 1 can be separated by the above process, and if necessary, the object liquid or the washing liquid can be additionally injected through the flow pipe 104 to form the liquid pipe 112 The rotating rod 126 is rotated to push up the inner plunger 128 to return the object liquid 1 located in the upper space of the inner plunger 128 inside the moving pipe body 112 again Mix with the target solution or wash solution.

Then, by centrifuging again and repeating the above-mentioned process, it becomes possible to obtain the objective liquid 1 having high purity. If the specific gravity of the objective liquid 1 is smaller than that of the separation liquid 2, the objective liquid 1 discharged through the distribution pipe 104 may be collected in another container.

It is also possible to use the component separating apparatus 100 with the flow channel 104 downward in the opposite direction. The object liquid 1 can be collected in the space formed between the inner plunger 128 and the moving pipe 112 when the specific gravity of the object liquid 1 is smaller than the separation liquid 2 and is equal. Conversely, when the specific gravity of the object liquid 1 is larger than the separation liquid 2 and the object liquid 1 is located at the lower side, the object liquid 1 discharged through the flow pipe 104 can be collected in another container Do.

As shown in FIG. 6, a connection pipe 134, in which a connection pipe filter 136 for filtering the liquid discharged through the flow pipe 104 is installed, may be used by being fastened to the flow pipe 104. As a result, impurities can be prevented from being mixed into the object liquid 1 discharged through the flow pipe 104.

Next, the component separating apparatus 101 according to the second embodiment of the present invention will be described with reference to Fig. The component separating apparatus 101 is provided with an internal filter 132 in an external plunger hole 122 formed in an external plunger 118. The inner filter 132 is in the form of a screen made of a material such as cloth, plastic, or metal. It is possible to prevent impurities from being introduced into the space formed between the inner plunger 128 and the moving pipe 112 by the inner filter 132. In particular, when the stem cells are separated, Can play a role.

It is also possible to use a combination of the component separator 100 of Embodiment 1 and the component separator 101 of Embodiment 2 when necessary. That is, in the case of stem cells, the component separation apparatus 100 of Example 1 may be used for the initial layer separation and several times of washing, and the component separation apparatus 101 of the second embodiment may be used for the final extraction.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it will be understood by those of ordinary skill in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. It can be understood that

1: Purpose solution 2: Separation solution
100, 101: Component separation device 102:
104: Distribution pipe 106: Distribution pipe periphery
108: scale 110: stopper
112: moving pipe body 114: outer plunger fixing portion
116: moving tube body expanding part 118: outer plunger
120: outer plunger upper surface 122: outer plunger hole
124: moving pipe threaded portion 126: rotating rod
128: inner plunger 130: rod handle
132: internal filter 134: connector
136: Connector filter

Claims

A body having a flow tube formed at one end thereof and having a space therein;
A movable conduit body having a back and forth inside the body, an outer plunger at one end, and a space communicating with the space of the body; And
And an outer plunger hole formed at one end of the outer plunger so as to communicate with the space of the main body and the space of the moving tube body, the outer plunger hole being threadedly coupled to the moving tube thread portion formed in the moving tube body, And a rotating rod on which an inner plunger having an outer diameter coinciding with the inner diameter is installed,
The moving pipe body moves relative to the main body in a state in which the moving pipe body extending portion formed at the other end of the moving pipe body is in contact with the inner wall surface of the main body,
Wherein the rotary rod is reciprocated in the space of the movable conduit when rotated, and the inner plunger is reciprocated in tight contact with the inner wall of the space of the movable conduit as one body with the rotary rod.

The component separating apparatus according to claim 1, wherein an upper surface of the outer plunger formed on the outer plunger toward the flow tube is formed to be inclined toward the inner plunger.

The component separating apparatus according to claim 1, wherein a threadless offset is formed in the rotating rod.

The apparatus of claim 1, wherein a stopper is formed on an inner wall of the main body so as to be in contact with the main body to limit movement of the main body.

The apparatus of claim 1, wherein an inner filter is disposed in the outer plunger hole.