KR101333788B1 - Separating vials and their uses - Google Patents

Abstract

The present invention relates to a container for centrifugal separation containing an inner packing member(110), a piston head(120), a plunger(130), a container body(140), and a piston head housing part(160). With one container for centrifugal separation of the present invention, a series of processes such as blood collection, centrifugal separation, and extraction is performed. A height difference(180) is created when a wedge-shaped piston head(120) and a piston head housing part(160) are in contact. The piston head(120) during centrifugation is lowered by the height difference by pressure to be wedge-coupled to the piston head housing(160) to prevent a sample inside the container body from leaking out of the container. The present invention utilizes distinctive technical features which are the wedge-shaped piston head(120) and the piston head housing part(160) to perform convenient and safe separation process of blood components. Unique combination of technical features is not limited to one separation method, and by the experience of a tester, innovative separation methods can continuously be created.

Description

Separating Vials and Their Uses

The present invention relates to a centrifugal vessel and its use.

The blood is used to comprehensively check the health state of the human body, and recently, the plasma, platelets, and blood cells are separated from the blood, and platelets and plasma, except for blood cells, are plated with blood plasma, which is diluted with platelets. Platelet Rich Plasma is used for a wide range of therapeutic purposes. For example, it is used in a wide range of therapeutic fields, such as wrinkles, pigment diseases, scars and other skin diseases such as dark circles, stretch marks, and sagging skin, cosmetic surgery, foot ulcers, and pain treatments, and researches in the field of use are continuing.

As such, blood has been widely used to separate blood from each component for various medical purposes. Among them, a blood centrifugation process using a specific gravity difference of blood components and a process using a specific composition are used. It is widely used. The centrifugation process is a process of performing delamination using the specific gravity difference of each component constituting the blood at a speed of about 3,000 rpm. When centrifuging the blood, the heaviest blood cells form a lower layer and leukocytes from above And platelet layers and plasma or serum layers thereon.

Conventionally, the separation of plasma, platelets, and blood cells from blood is performed by first bleeding blood of a human body using a syringe, and then transferring the blood into at least a primary and a secondary container as a centrifugation kit, and centrifugation by a centrifuge. Obtained by separation. In more detail, the blood sample collected from the human body is injected into a syringe-type test tube, and the test tube into which the blood is injected is centrifuged using a centrifuge. When the centrifugation is completed, the red blood cells descend to the lower part of the test tube and the plasma is placed above the red blood cells. Next, insert the needle of the syringe into the test tube to inhale and extract the plasma located above the red blood cells. Next, centrifugation of the extracted plasma once again collects PRP at the bottom of the syringe tube and collects the rest of the plasma including PPP (Platelet poor plasma) above the PRP. The syringe is then used to inhale the remainder of the plasma, including the PPP, located above the test tube, discharge it from the test tube, and extract the remaining PRP into the test tube with a new syringe.

Conventional syringe-type blood component separation apparatus using centrifugation is equipped with a rubber packing or a strong piston head at the bottom of the container to prevent blood from leaking out of the container. However, as described above, such a container requires a blood collection using a separate syringe, and the blood collected after the blood collection has been inconvenient to be transferred to the centrifugal container, and a separate component for collecting separated components even after centrifugation. Need a device.

Therefore, the present inventors have developed a centrifugal container designed to simultaneously collect blood and extract blood components while maintaining a conventional syringe form for blood centrifugation, and the centrifugal container of the present invention uses a single container for blood collection and centrifugation. And since a series of extraction processes are possible, blood components can be separated more conveniently and quickly.

Numerous papers and patent documents are referenced and cited throughout this specification. The disclosures of the cited papers and patent documents are incorporated herein by reference in their entirety to better understand the state of the art to which the present invention pertains and the content of the present invention.

The present inventors earnestly tried to develop a centrifugal container designed to simultaneously collect blood and extract blood components while maintaining a conventional syringe form for centrifugation of a sample. As a result, a series of processes of blood collection, centrifugation and extraction are possible using one centrifugation vessel, and a centrifugation vessel has been developed that can obtain a sample fraction more conveniently and quickly.

It is therefore an object of the present invention to provide a centrifugal vessel.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.

According to one aspect of the present invention, the present invention provides a centrifugation vessel 100 comprising:

(a) an inner packing 110 which prevents leakage of a sample to be separated contained in the centrifugal container and is retractable in the centrifugal container;

(b) a wedge-shaped piston head 120 that engages with the inner packing 110;

(c) a plunger (130) coupled with the piston head (120) for generating pressurized or negative pressure in the centrifugal vessel while advancing in the centrifugal vessel;

(d) a main body 140 having a distribution pipe through which a sample is allowed to enter and exit on one side, and a passage through which the plunger 130 can be retracted and formed on the other side; And

(e) The piston head receiving portion (160) is formed in the inner lower portion of the main body 140, and closely coupled to the piston head when the piston head (120) is lowered by pressing.

The present inventors earnestly tried to develop a centrifugal container designed to simultaneously collect blood and extract blood components while maintaining a conventional syringe form for centrifugation of a sample. As a result, a series of processes of blood collection, centrifugation and extraction are possible using one centrifugation vessel, and a centrifugation vessel has been developed that can obtain a sample fraction more conveniently and quickly.

In the conventional syringe-type centrifugal container using a centrifugal separator to prevent the sample from leaking out of the container by mounting a rubber packing or a strong piston head at the bottom of the container, the syringe head coupled to the container has a strong adhesive force Not only is it difficult to collect blood using the centrifugal container itself, but the rubber packing bonded to the lower part of the container cannot be moved back and forth in the container, so it is necessary to separate the fractions using a separate separation means (for example, a syringe). By using one centrifugal container, blood collection was possible, and a centrifugal container was developed in which the fractions could be easily separated and obtained while allowing the sample to leak during centrifugation.

The centrifugal vessel of the present invention can be applied to any sample that can be separated or concentrated by a density gradient centrifugation method, and is applicable to, for example, blood, plasma, serum or cell culture fluids, which are biological samples. If the sample is blood, the fraction obtained by centrifugation may be plasma, serum, platelets, platelet rich plasma (PRP) or platelet poor plasma (PPP). Centrifugation of the collected blood to remove red blood cells and platelet and growth factor-rich buffy coat (Buffy coat) layer is a high concentration of platelets and growth factors Say.

The centrifugal container 100 of the present invention may be manufactured in any shape known in the art, for example, a cylindrical shape, a cylindrical shape having an upper dome shape, and a general syringe shape. According to a specific embodiment of the present invention, the centrifugal container 100 of the present invention has a generally cylindrical generally syringe-shaped body 140, and the retractable plunger 130 and the piston head 120 inside the body. Can be inserted. The main body is formed with a distribution pipe through which the sample is allowed to enter and exit, and a passage through which the plunger 130 can be retracted and formed on the other side. Sterilized syringe needles may be mounted in the distribution tube during blood collection, and a stopper 150 may be mounted in the centrifugal separation. When using the centrifugal container of the present invention, blood collection, centrifugation, and fractionation may be performed. The advantage is that it can be extracted.

  The piston head 120 has a wedge shape, and an inner packing 110 is coupled to an upper portion of the piston head, and the inner packing prevents leakage of a sample to be separated contained in the centrifugal container and can be retracted in the centrifugal container. To be mounted.

The plunger 130 may generate pressure or negative pressure in the centrifugal container while advancing in the centrifugal container, and form a female thread or a male thread on each of the wedge-shaped piston head 120 and the plunger 130. Can be screwed together. When the centrifugal separation, the piston head 120 and the plunger 130 should be separated, and the plunger may be separated by screwing or by applying physical pressure to the plunger attached to the piston head 120. Can be separated for cutting

On the other hand, the piston head receiving portion 160 is formed in the inner lower portion of the main body 140, can be in close contact with the piston head when the piston head 120 is lowered by pressing. The piston head receiving portion 160 may be screwed with the ring 170 mounted on the inner lower portion of the main body 140, thereby being fixed to the main body. The ring 170 more firmly fixes the piston head receiving portion 160 to the body so that the internal sample does not leak during centrifugation. The lower end portion of the piston head 120 has a shape that is narrow in the downward direction, and the inside of the piston head receiving portion 160 has a shape that is narrow in the downward direction.

When the piston head 120 and the piston head receiving portion 160 is in contact with the height difference 180 is formed, the centrifugal piston head 120 is lowered by the height difference 180 by pressing the piston By tightly coupling with the head receiving portion 160, it is possible to prevent the leakage of the sample inside the main body 140. The height difference 180 is in the range of 0.5-10 mm, preferably 0.5-8 mm, more preferably 0.5-5 mm, even more preferably 0.5-3 mm, even more preferably 1-3 mm.

In the centrifugal container 100 of the present invention, the inner packing 110 and the piston head receiving portion 160 may be formed of an elastic material. Downward pressure is applied to the piston head 120 during centrifugation, and the piston head presses the piston head receiving portion 160 made of an elastic material, thereby preventing leakage of a sample that may occur during centrifugation. It can prevent.

According to a preferred embodiment of the present invention, the centrifugal container 100 of the present invention may further include a stopper 150 is formed to open and close the distribution pipe of the upper portion of the main body 140.

The features and advantages of the present invention are summarized as follows:

(a) The present invention provides a centrifugal container including an inner packing (110), a piston head (120), a plunger (130), a body (140), and a piston head receiver (160).

(b) In the case of using the centrifugal container of the present invention, there is an advantage that a series of processes of blood collection, centrifugation and extraction are possible using one centrifugal container.

(c) Wedge-shaped piston head 120 and the piston head receiving portion 160 of the configuration of the present invention when the height difference 180 is formed in contact, the centrifugal separation when the piston head 120 is pressurized by the height difference It is possible to prevent the leakage of the sample inside the main body 140 by descending by 180 to be in close contact with the piston head receiving portion 160.

(d) The present invention is very convenient and safe to separate blood components by the unique technical features of the above-described wedge-shaped piston head 120 and the piston head receiving portion 160, the combination of the unique core technical features is one It is not limited to the method of separation, but the experience of the investigator provides room for continued creation of creative methods of separation.

1 shows a perspective view of a centrifugal vessel 100 according to a preferred embodiment of the present invention.
2 and 3 show the constitution of the centrifugal separation vessel according to a preferred embodiment of the present invention, and the combined flow chart of each constitution.
4 shows an internal cross-sectional view of a centrifugal vessel 100 according to a preferred embodiment of the present invention. The piston head 120 and the plunger 130 are screwed (left), and the centrifugal separation as shown in the center picture, during centrifugation, the piston head 120 and the plunger 130 are closely coupled (right). , The piston head 120 is lowered by the height difference 180 when pressurized by centrifugation.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Example

Example 1: Preparation of Centrifuge Vessel

The centrifugal vessel 100 has a substantially cylindrical body 140, and the retractable plunger 130 and the piston head 120 may be inserted into the body. The main body is formed with a distribution pipe through which the sample is allowed to enter and exit, and a passage through which the plunger 130 can be retracted and formed on the other side. In the case of blood collection, a sterile syringe needle may be mounted in the distribution pipe, and a stopper 150 may be mounted in centrifugation.

The piston head 120 has a wedge shape, and the upper portion of the piston head prevents leakage of a sample to be separated contained in the centrifugal container, and couples an inner packing 110 capable of moving back and forth in the centrifugal container. The inner packing and the piston head were made of rubber material.

   The plunger 130 may generate pressure or negative pressure in the centrifugal container while advancing in the centrifugal container, and form a female thread or a male thread on each of the wedge-shaped piston head 120 and the plunger 130. It can be screwed together.

On the other hand, the piston head receiving portion 160 is formed in the inner lower portion of the main body 140, and is in close contact with the piston head and wedge coupling when the piston head 120 is lowered by pressing. The main body 140 is screwed with the ring 170 mounted on the inner lower portion of the main body 140, thereby fixing the ring 170 to the main body 140. The piston head receiving portion 160 and the ring 170 may be manufactured in an assemblable form and may be combined by simple assembly of each component. The lower end portion of the piston head 120 has a shape that is narrow in the downward direction, and the inside of the piston head receiving portion 160 has a shape that is narrow in the downward direction.

The piston head 120 and the piston head receiving portion 160 formed a height difference 180 of about 2 mm when contacted, and the piston head 120 was pressurized by the height difference 180 when pressurized during centrifugation. By lowering and wedge coupling with the piston head receiving portion 160, leakage of the sample inside the main body 140 can be prevented. The piston head 120 and the piston head receiving portion 160 is in close contact with the wedge coupling, the more closely coupled as the pressure occurs during centrifugation.

On the other hand, the centrifugal container 100 is equipped with a cap 150 that is formed to be opened and closed on the main body 140.

Example  2: Separation of Blood Components Using Centrifugal Containers

After mounting a sterile syringe needle on one side of the centrifugal vessel of the present invention, the anticoagulant was aspirated with a syringe before blood was collected. An appropriate amount of anticoagulant is about 10% of the amount of blood to be collected. Blood was collected from humans with a syringe inhaled with anticoagulant. After the needle was removed from the centrifugal container containing blood and sealed with a stopper 150, the plunger 130 screwed with the piston head 120 was removed.

Next, the vessel of the present invention in which the blood was injected was centrifuged for about 5 minutes at a speed of 3000 rpm with the distribution tube upward. At this time, the red blood cells are lowered to the lower part of the main body 140, and the plasma is located upward from the center of the main body which is higher than the red blood cells.

Due to the height difference 180 generated when the piston head 120 and the piston head receiving portion 160 contact, the piston head 120 is as much as the height difference 180 when the pressure caused by the centrifugal separation occurs. Lowered, the piston head 120 coupled to the inner packing 110 is in close contact with the piston head receiving portion 160 that is present in the inner lower portion of the main body was confirmed that the blood does not leak.

When the blood is centrifuged, the red blood cell layer is located at the bottom, the buffy coat layer is located above, the platelet rich plasma (PRP) is located above, and the platelet deficient plasma layer (PPP) above. plasma).

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

Centrifuge Vessel: 100
Inner Packing: 110
Piston Head: 120
Plunger: (130)
Body: 140
Plug: (150)
Piston Head Receptacle: (160)
Ring: 170

Claims (10)

Centrifugal vessel 100 comprising:
(a) an inner packing 110 which prevents leakage of a sample to be separated contained in the centrifugal container and is retractable in the centrifugal container;
(b) a wedge-shaped piston head 120 that engages with the inner packing 110;
(c) a plunger (130) coupled with the piston head (120) for generating pressurized or negative pressure in the centrifugal vessel while advancing in the centrifugal vessel;
(d) a main body 140 having a distribution pipe through which a sample is allowed to enter and exit on one side, and a passage through which the plunger 130 can be retracted and formed on the other side;
(e) a piston head accommodating part 160 formed in the inner lower portion of the main body 140 and tightly coupled to the piston head when the piston head 120 is lowered by pressing; And
(f) a ring (170) mounted on the inner lower portion of the main body (140) and screwed with the piston head receiving portion (160);
When the piston head 120 and the piston head receiving portion 160 is in contact with the height difference 180 is formed, the centrifugal piston head 120 is lowered by the height difference 180 by pressing the piston Centrifugal container characterized in that the wedge is coupled to the head receiving portion 160 to prevent leakage of the sample inside the main body (140).
The centrifugal container according to claim 1, wherein the centrifugal container (100) further comprises a stopper (150) formed to be opened and closed on the main body (140).
delete The centrifugal container according to claim 1, wherein the plunger (130) is screwed or cut apart from the piston head (120).
The centrifugal container according to claim 1, wherein the lower end portion of the piston head (120) has a shape that narrows in a downward direction.
The centrifugal container according to claim 1, wherein the inside of the piston head receiving portion (160) has a shape that narrows in a downward direction.
The centrifugal container according to claim 1, wherein the inner packing (110) and the piston head receiving portion (160) are made of an elastic material.
8. The centrifugal container according to claim 7, wherein the inner packing (110) and the piston head receiving portion (160) are formed of a material including rubber.
delete The centrifugal container according to claim 1, wherein the sample is blood, plasma, serum or cell culture fluid.

Images