CN103566629A - Method and device for separating and extracting platelet rich blood - Google Patents
Method and device for separating and extracting platelet rich blood Download PDFInfo
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- CN103566629A CN103566629A CN201310539497.XA CN201310539497A CN103566629A CN 103566629 A CN103566629 A CN 103566629A CN 201310539497 A CN201310539497 A CN 201310539497A CN 103566629 A CN103566629 A CN 103566629A
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Abstract
The invention discloses a method and a device for separating and extracting a platelet rich in blood. Venous blood is extracted by a medical injector, is injected into a device, and is separated by utilizing a single device, and the platelet cell is extracted for later use, wherein the device comprises a PRP (platelet rich plasma) storage chamber, a plasma storage chamber, a locking lever spinner and an erythrocyte storage chamber from top to bottom. The method and the device for separating and extracting the platelet rich in the blood overcome the defects that a traditional platelet-rich blood separating and extracting operation uses more tools, is not convenient to operate and easily causes pollution, are simple in structure and convenient to operate, can complete the whole separation process of blood in only one device, and have a remarkable effect.
Description
Technical field
The present invention relates to technical field of medical instruments, especially a kind of blood rich platelet separating and extracting process and device.
Background technology
Upper a whole set of a lot of devices, test tube, the syringe etc. will used in the process of doing blood rich platelet separation and Extraction of medical treatment at present, in operation, it is also very complex, to pass through multiple injection, centrifugal, extraction, very inconvenient, and easily cause pollution, therefore this kind of Method and kit for all will improve, and makes whole operating procedure simplification, pollution-freeization.
Summary of the invention
The technical problem to be solved in the present invention is: in order to solve the problem that in above-mentioned background technology, existing blood rich platelet separation and Extraction operation exists, provide a kind of improved blood rich platelet separating and extracting process and device.
The technical solution adopted in the present invention is: a kind of blood rich platelet separating and extracting process, and concrete steps are:
A, with 50ml injector for medical purpose venous blood samples, this device is upright, the Capsule that filters passage is opened, and from blood hand-hole, is pierced through silica gel plug and inject venous blood in blood plasma locker room, blood is by inflow red blood cell locker room, blood plasma locker room;
B, get the ACD-A anti-coagulants (anti-coagulants and blood ratio 1:10) of 5ml simultaneously, by blood hand-hole, inject, close filtration passage;
C, this device is put on centrifuge to the centrifugal 10min of centrifugal force with 200g, blood will layering, lower floor's liquid level is red blood cell, top liquid level is serum, regulate the lid of red blood cell locker room top serum to be adjusted in to the sealing place top of blood plasma entrance hole, in order to guarantee that red blood cell do not infiltrate serum, the serum liquid level that stays 3mm height left and right is below sealing place of blood plasma entrance hole, and rotation PRP/ red blood cell securing rod swing handle makes PRP securing rod enter blood plasma entrance hole and makes its sealing isolation;
D, this device is inverted, the hollow hole by the serum layer isolating by blood plasma locker room enters PRP locker room;
E, this device is put on centrifuge to the centrifugal 10min of centrifugal force with 200g, blood will layering, bottom is platelet cell, top is impurity layer, the lid that regulates PRP locker room, is adjusted to the impurity layer on top on the serum entrance hole of PRP locker room, in order to guarantee that free from admixture refers and synthesizes, the platelet cell layer of 2mm liquid level is also modulated on serum entrance hole simultaneously, then PRP securing rod is adjusted to taper hole sealing partition;
F, tear the protection foil film of the tops of PRP locker room off, injector for medical purpose is thrust to silica gel plug ZhiPRP locker room, extract platelet rich plasma standby.
Above-mentioned blood rich platelet separating and extracting process device used: comprise from top to bottom PRP locker room, blood plasma locker room, PRP/ red blood cell securing rod swing handle and red blood cell locker room, described blood plasma locker room respectively YuPRP locker room and PRP/ red blood cell securing rod swing handle is threaded, and threaded connection place is provided with silica gel sealing ring, PRP/ red blood cell securing rod swing handle and the fixed seal connection of red blood cell locker room, junction is communicated with by blood plasma entrance hole, between PRP locker room and blood plasma locker room, be also provided with the serum entrance hole of a taper, in described blood plasma locker room, be also provided with a PRP securing rod, this PRP securing rod is fixedly connected with and follows its motion with blood plasma locker room, and this PRP securing rod runs through the space between serum entrance hole and blood plasma entrance hole from top to bottom successively, the two ends caliber size of described PRP securing rod coincide with the aperture size of described serum entrance hole and blood plasma entrance hole respectively, while making PRP securing rod enter in corresponding entrance hole, can seal the space up and down of the corresponding entrance hole of isolation, in described PRP locker room and red blood cell locker room, be all threaded connection lid, threaded connection place seals by silica gel sealing ring.
Further, in the lid of PRP of the present invention locker room, sealing is provided with silica gel plug and on silica gel plug, is coated with protection foil film.
Further, on the sidewall of PRP of the present invention locker room, be provided with two symmetrical holes, one is blood hand-hole, another is to filter passage, on described blood hand-hole, by double-colored pair of material, be injected with a plug, filter on passage and be pressed with a ventilative resistance mycoderm by silica gel pressure ring, and be provided with a dismountable Capsule.
The invention has the beneficial effects as follows: tool using that a kind of blood rich platelet separating and extracting process of the present invention and device have overcome traditional blood rich platelet separation and Extraction operation existence is many, inconvenient operation, the defect of pollution of easily causing, simple in structure, easy to operate, only need in a device, can complete the whole separation process of blood, there is significant progress.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the present invention is further described.
Fig. 1 is the structural representation of the preferred embodiments of the present invention;
Tu Zhong 1.PRP locker room, 1-1. serum entrance hole ，2. blood plasma locker room, 2-1.PRP securing rod, 3.PRP/ red blood cell securing rod swing handle, 3-1. blood plasma entrance hole ，4. red blood cell locker room, 5. silica gel sealing ring, 6. silica gel plug, 7. blood hand-hole, 8. filters passage.
The specific embodiment
The present invention is further detailed explanation with preferred embodiment by reference to the accompanying drawings now.These accompanying drawings are the schematic diagram of simplification, basic structure of the present invention is only described in a schematic way, so it only show the formation relevant with the present invention.
The present invention's a kind of blood rich platelet separating and extracting process as shown in Figure 1 and the embodiment of device,
The concrete steps of described method are:
A, with 50ml injector for medical purpose venous blood samples, this device is upright, the Capsule that filters passage 8 is opened, and from blood hand-hole 7, is pierced through silica gel plug and inject venous blood in blood plasma locker room 2, blood is by 2 inflow red blood cell locker rooms 4, blood plasma locker room;
B, get the ACD-A anti-coagulants (anti-coagulants and blood ratio 1:10) of 5ml simultaneously, by blood hand-hole 7, inject, close and filter passage 8;
C, this device is put on centrifuge to the centrifugal 10min of centrifugal force with 200g, blood will layering, lower floor's liquid level is red blood cell, top liquid level is serum, regulate the lid of red blood cell locker room 4 top serum to be adjusted in to the sealing place top of blood plasma entrance hole 3-1, in order to guarantee red blood cell, do not infiltrate serum, the serum liquid level that stays about 3mm height is below sealing place of blood plasma entrance hole 3-1, and rotation PRP/ red blood cell securing rod swing handle 3 makes PRP securing rod 2-1 enter blood plasma entrance hole 3-1 and makes its sealing isolation;
D, this device is inverted, the hollow hole by the serum layer isolating by blood plasma locker room 2 enters PRP locker room 1;
E, this device is put on centrifuge to the centrifugal 10min of centrifugal force with 200g, blood will layering, bottom is platelet cell, top is impurity layer, the lid that regulates PRP locker room 1, is adjusted to the impurity layer on top on the serum entrance hole 1-1 of PRP locker room 1, in order to guarantee that free from admixture refers and synthesizes, the platelet cell layer of 2mm liquid level is also modulated on serum entrance hole 1-1 simultaneously, then PRP securing rod 2-1 is adjusted to taper hole sealing partition;
F, tear the protection foil film of the tops of PRP locker room 1 off, injector for medical purpose is thrust to silica gel plug 6 ZhiPRP locker rooms 1, extract platelet rich plasma standby.
Above-mentioned blood rich platelet separating and extracting process device used: comprise from top to bottom PRP locker room (1), blood plasma locker room (2), PRP/ red blood cell securing rod swing handle (3) and red blood cell locker room (4), described difference YuPRP locker room (1), blood plasma locker room (2) and PRP/ red blood cell securing rod swing handle (3) are threaded, and threaded connection place is provided with silica gel sealing ring (5), PRP/ red blood cell securing rod swing handle (3) and red blood cell locker room (4) fixed seal connection, junction is communicated with by blood plasma entrance hole (3-1), between PRP locker room (1) and blood plasma locker room (2), be also provided with the serum entrance hole (1-1) of a taper, in described blood plasma locker room, (2) are also provided with a PRP securing rod (2-1), this PRP securing rod (2-1) is fixedly connected with and follows its motion with blood plasma locker room (2), and this PRP securing rod (2-1) runs through the space between serum entrance hole (1-1) and blood plasma entrance hole (3-1) from top to bottom successively, the two ends caliber size of described PRP securing rod (2-1) coincide with the aperture size of described serum entrance hole (1-1) and blood plasma entrance hole (3-1) respectively, while making PRP securing rod (2-1) enter in corresponding entrance hole, can seal the space up and down of the corresponding entrance hole of isolation, on described PRP locker room (1) and red blood cell locker room (4), be all threaded connection lid, threaded connection place seals by silica gel sealing ring.
Further, in the lid of PRP of the present invention locker room (1), sealing is provided with silica gel plug (6) and is coated with protection foil film on silica gel plug (6).
Further, on the sidewall of PRP of the present invention locker room (1), be provided with two symmetrical holes, one is blood hand-hole (7), another is to filter passage (8), described blood hand-hole (7) is upper is injected with a plug by double-colored pair of material, filter passage (8) and above by silica gel pressure ring, be pressed with a ventilative resistance mycoderm, and be provided with a dismountable Capsule.
Just the specific embodiment of the present invention of describing in above description, various not illustrating is construed as limiting flesh and blood of the present invention, person of an ordinary skill in the technical field read after description can to before the described specific embodiment make an amendment or be out of shape, and do not deviate from essence of an invention and scope.
Claims (4)
1. a blood rich platelet separating and extracting process, is characterized in that: concrete steps are:
A, use injector for medical purpose venous blood samples, this device is upright, the Capsule that filters passage (8) is opened, and from blood hand-hole (7), pierced through silica gel plug and inject venous blood in blood plasma locker room (2), blood flows into red blood cell locker room (4) by blood plasma locker room (2);
B, the ACD-A anti-coagulants of simultaneously getting, anti-coagulants and blood ratio 1:10, inject by blood hand-hole (7), closes and filter passage (8);
C, that this device is put in to centrifuge is centrifugal, blood will layering, lower floor's liquid level is red blood cell, top liquid level is serum, regulate the lid of red blood cell locker room (4) top serum to be adjusted in to the sealing place top of blood plasma entrance hole (3-1), in order to guarantee red blood cell, do not infiltrate serum, stay the serum liquid level of 2mm-3mm height in the below of sealing place of blood plasma entrance hole (3-1), rotation PRP/ red blood cell securing rod swing handle (3) makes PRP securing rod (2-1) enter blood plasma entrance hole (3-1) and makes its sealing isolation;
D, this device is inverted, the hollow hole by the serum layer isolating by blood plasma locker room (2) enters PRP locker room (1);
E, this device is put on centrifuge centrifugal, blood will layering, bottom is platelet cell, top is impurity layer, the lid that regulates PRP locker room (1), is adjusted to the impurity layer on top on the serum entrance hole (1-1) of PRP locker room (1), in order to guarantee that free from admixture refers and synthesizes, the platelet cell layer of 2mm-3mm liquid level is also modulated on serum entrance hole (1-1) simultaneously, then PRP securing rod (2-1) is adjusted to taper hole sealing partition;
F, tear the protection foil film of the tops of PRP locker room 1 off, injector for medical purpose is thrust to silica gel plug (6) ZhiPRP locker room (1), extract platelet rich plasma standby.
2. a blood rich platelet separating extraction device, it is characterized in that: comprise from top to bottom PRP locker room (1), blood plasma locker room (2), PRP/ red blood cell securing rod swing handle (3) and red blood cell locker room (4), described difference YuPRP locker room (1), blood plasma locker room (2) and PRP/ red blood cell securing rod swing handle (3) are threaded, and threaded connection place is provided with silica gel sealing ring (5), PRP/ red blood cell securing rod swing handle (3) and red blood cell locker room (4) fixed seal connection, junction is communicated with by blood plasma entrance hole (3-1), between PRP locker room (1) and blood plasma locker room (2), be also provided with the serum entrance hole (1-1) of a taper, in described blood plasma locker room, (2) are also provided with a PRP securing rod (2-1), this PRP securing rod (2-1) is fixedly connected with and follows its motion with blood plasma locker room (2), and this PRP securing rod (2-1) runs through the space between serum entrance hole (1-1) and blood plasma entrance hole (3-1) from top to bottom successively, the two ends caliber size of described PRP securing rod (2-1) coincide with the aperture size of described serum entrance hole (1-1) and blood plasma entrance hole (3-1) respectively, while making PRP securing rod (2-1) enter in corresponding entrance hole, can seal the space up and down of the corresponding entrance hole of isolation, on described PRP locker room (1) and red blood cell locker room (4), be all threaded connection lid, threaded connection place seals by silica gel sealing ring.
3. a kind of blood rich platelet separating extraction device as claimed in claim 2, is characterized in that: in the lid of described PRP locker room (1), sealing is provided with silica gel plug (6) and is coated with protection foil film on silica gel plug (6).
4. a kind of blood rich platelet separating extraction device as claimed in claim 2, it is characterized in that: on the sidewall of described PRP locker room (1), be provided with two symmetrical holes, one is blood hand-hole (7), another is to filter passage (8), described blood hand-hole (7) is upper is injected with a plug by double-colored pair of material, filter passage (8) and above by silica gel pressure ring, be pressed with a ventilative resistance mycoderm, and be provided with a dismountable Capsule.
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| CN201310539497.XA CN103566629B (en) | 2013-11-04 | 2013-11-04 | Method and device for separating and extracting platelet rich blood |
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| CN201310539497.XA CN103566629B (en) | 2013-11-04 | 2013-11-04 | Method and device for separating and extracting platelet rich blood |
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| CN103566629A true CN103566629A (en) | 2014-02-12 |
| CN103566629B CN103566629B (en) | 2015-05-06 |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016192502A1 (en) * | 2015-06-01 | 2016-12-08 | 宋东范 | Extraction device of blood separator for extracting growth factor from platelet rich plasma |
| WO2017000851A1 (en) * | 2015-07-02 | 2017-01-05 | 浙江康慈医疗科技有限公司 | Integrated autologous platelet-rich gel preparation kit |
| CN106582908A (en) * | 2016-11-30 | 2017-04-26 | 深圳市达科为生物工程有限公司 | Platelet-rich plasma rapid separating device and method |
| CN110252525A (en) * | 2019-04-22 | 2019-09-20 | 福州比华利美容医院有限公司 | Blood ingredient separator tool |
| CN110639712A (en) * | 2019-10-11 | 2020-01-03 | 武汉济源高科技有限公司 | Autologous platelet-rich plasma separation preparation device |
| CN111254045A (en) * | 2018-11-30 | 2020-06-09 | 瑞崴制造股份有限公司 | Kit for separating and concentrating humoral cells containing stem cells |
| CN112058174A (en) * | 2020-09-24 | 2020-12-11 | 北京大学第三医院（北京大学第三临床医学院） | Platelet-rich plasma (PRP) spray gun device |
| CN113546450A (en) * | 2021-07-27 | 2021-10-26 | 伯格曼(江苏)医疗科技有限公司 | Multipurpose platelet and plasma preparation device |
| CN114452684A (en) * | 2020-11-10 | 2022-05-10 | 菲尔德森(江苏)生物技术有限公司 | Platelet-rich plasma extraction and separation kit and extraction method |
| CN114870436A (en) * | 2022-06-21 | 2022-08-09 | 浙江保尔曼生物科技有限公司 | Platelet-rich plasma preparation device and preparation method thereof |
| CN114939345A (en) * | 2022-06-21 | 2022-08-26 | 浙江保尔曼生物科技有限公司 | Platelet-rich plasma preparation device and operation method thereof |
| CN115445683A (en) * | 2022-09-24 | 2022-12-09 | 北京市美嘉亿盛医疗器械股份有限公司 | PRP test tube |
| CN115475416A (en) * | 2022-09-13 | 2022-12-16 | 山东同齐医学科技有限公司 | Platelet-rich plasma preparation mechanism |
| CN117126716A (en) * | 2023-09-06 | 2023-11-28 | 浏阳市三力医用科技发展有限公司 | Disposable special centrifuge tube for platelet-rich plasma and accessory thereof |
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Cited By (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016192502A1 (en) * | 2015-06-01 | 2016-12-08 | 宋东范 | Extraction device of blood separator for extracting growth factor from platelet rich plasma |
| WO2017000851A1 (en) * | 2015-07-02 | 2017-01-05 | 浙江康慈医疗科技有限公司 | Integrated autologous platelet-rich gel preparation kit |
| CN106582908A (en) * | 2016-11-30 | 2017-04-26 | 深圳市达科为生物工程有限公司 | Platelet-rich plasma rapid separating device and method |
| CN106582908B (en) * | 2016-11-30 | 2019-09-13 | 深圳市达科为生物工程有限公司 | A kind of platelet rich plasma fast separation device and method |
| CN111254045A (en) * | 2018-11-30 | 2020-06-09 | 瑞崴制造股份有限公司 | Kit for separating and concentrating humoral cells containing stem cells |
| CN111254045B (en) * | 2018-11-30 | 2023-07-11 | 瑞崴制造股份有限公司 | Body fluid cell separation and concentration kit containing stem cells |
| CN110252525A (en) * | 2019-04-22 | 2019-09-20 | 福州比华利美容医院有限公司 | Blood ingredient separator tool |
| CN110639712A (en) * | 2019-10-11 | 2020-01-03 | 武汉济源高科技有限公司 | Autologous platelet-rich plasma separation preparation device |
| CN112058174B (en) * | 2020-09-24 | 2022-05-13 | 北京大学第三医院（北京大学第三临床医学院） | Platelet-rich plasma (PRP) spray gun device |
| CN112058174A (en) * | 2020-09-24 | 2020-12-11 | 北京大学第三医院（北京大学第三临床医学院） | Platelet-rich plasma (PRP) spray gun device |
| CN114452684B (en) * | 2020-11-10 | 2024-10-11 | 菲尔德森(江苏)生物技术有限公司 | Platelet-rich plasma extraction and separation set and extraction method |
| CN114452684A (en) * | 2020-11-10 | 2022-05-10 | 菲尔德森(江苏)生物技术有限公司 | Platelet-rich plasma extraction and separation kit and extraction method |
| CN113546450A (en) * | 2021-07-27 | 2021-10-26 | 伯格曼(江苏)医疗科技有限公司 | Multipurpose platelet and plasma preparation device |
| CN113546450B (en) * | 2021-07-27 | 2022-06-28 | 伯格曼(江苏)医疗科技有限公司 | Multipurpose platelet and plasma preparation device |
| CN114870436A (en) * | 2022-06-21 | 2022-08-09 | 浙江保尔曼生物科技有限公司 | Platelet-rich plasma preparation device and preparation method thereof |
| CN114939345A (en) * | 2022-06-21 | 2022-08-26 | 浙江保尔曼生物科技有限公司 | Platelet-rich plasma preparation device and operation method thereof |
| CN115475416A (en) * | 2022-09-13 | 2022-12-16 | 山东同齐医学科技有限公司 | Platelet-rich plasma preparation mechanism |
| CN115475416B (en) * | 2022-09-13 | 2024-05-31 | 山东同齐医学科技有限公司 | Platelet-rich plasma preparation mechanism |
| CN115445683B (en) * | 2022-09-24 | 2023-08-22 | 北京市美嘉亿盛医疗器械股份有限公司 | PRP test tube |
| CN115445683A (en) * | 2022-09-24 | 2022-12-09 | 北京市美嘉亿盛医疗器械股份有限公司 | PRP test tube |
| CN117126716A (en) * | 2023-09-06 | 2023-11-28 | 浏阳市三力医用科技发展有限公司 | Disposable special centrifuge tube for platelet-rich plasma and accessory thereof |
| CN117126716B (en) * | 2023-09-06 | 2024-04-05 | 浏阳市三力医用科技发展有限公司 | Disposable special centrifuge tube for platelet-rich plasma and accessory thereof |
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