CN207227399U - A kind of large volume urine DNA/RNA extraction systems - Google Patents
A kind of large volume urine DNA/RNA extraction systems Download PDFInfo
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- CN207227399U CN207227399U CN201721062752.6U CN201721062752U CN207227399U CN 207227399 U CN207227399 U CN 207227399U CN 201721062752 U CN201721062752 U CN 201721062752U CN 207227399 U CN207227399 U CN 207227399U
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- dna
- rna
- urine
- extractors
- container
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- 210000002700 urine Anatomy 0.000 title claims abstract description 90
- 238000007400 DNA extraction Methods 0.000 title claims abstract description 19
- 238000002123 RNA extraction Methods 0.000 title claims abstract description 19
- 238000000605 extraction Methods 0.000 claims description 10
- 230000027939 micturition Effects 0.000 claims description 9
- 238000005119 centrifugation Methods 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 7
- 239000011800 void material Substances 0.000 claims description 6
- 238000005086 pumping Methods 0.000 claims description 5
- 230000000740 bleeding effect Effects 0.000 claims description 3
- 238000009434 installation Methods 0.000 claims description 3
- 235000003140 Panax quinquefolius Nutrition 0.000 abstract description 6
- 240000005373 Panax quinquefolius Species 0.000 abstract description 6
- 238000004458 analytical method Methods 0.000 description 4
- 210000002445 nipple Anatomy 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 239000003463 adsorbent Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 230000007547 defect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000003708 urethra Anatomy 0.000 description 1
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Abstract
The utility model discloses a kind of large volume urine DNA/RNA extraction systems, including DNA/RNA extractors, negative pressure aspirator, container, the DNA/RNA extractors include extractor body and vacuum suction connection mouth, and the top of wherein extractor body is provided with Sheng urine container to coordinate into catheter and installs；The lower end of the extractor body is provided with out catheter, this goes out catheter and connects the vacuum suction connection mouth by broken part, and the vacuum suction connection mouth is connected by dismountable Hermetical connecting structure with negative pressure aspirator；DNA/RNA extractors are used to adsorb the DNA/RNA in urine；The container is used to be set with the extractor body, and contains the DNA/RNA isolated in extractor body.The utility model is easy to use, reduces the secondary pollution of DNA/RNA.
Description
Technical field
It the utility model is related to technical field of medical equipment, and in particular to a kind of large volume urine DNA/RNA extraction systems.
Background technology
DNA/RNA in urine comes from the cell to come off in urethra, and molecular biology mechanism is carried out with urine DNA/RNA
Research and clinical diagnosis have the advantages of many special：1) urine collecting thing is non-intervention, non-invasive.2) extracted from urine
DNA/RNA is more simpler than extracting DNA/RNA from blood.
Urine DNA/RNA extraction elements of the prior art, Sheng urine container, DNA/RNA extractors, connecting leg, sealing connect
Head is Split type structure, is combined, combines in use, urine container, DNA/RNA extractors, connecting leg, seal nipple will must be contained
After the completion of, DNA/RNA extractors are connected by negative pressure aspirator and extract urine, DNA/RNA is extracted by DNA/RNA extractors,
After the completion of extraction, then urine container will be contained, connecting leg, seal nipple are taken off, then by DNA/RNA extractors be put into container into
The defects of centrifuging treatment of row DNA/RNA, the prior art is separation operation process complexity, inconvenient to use, is easily caused
Secondary pollution, during negative-pressure ward, since part is too many, without sealing is easily guaranteed that, is unfavorable for negative pressure aspirator pumping
Take urine.
Utility model content
In view of at least one defect of the prior art, the purpose of this utility model is to provide a kind of large volume urine DNA/
RNA extraction systems, DNA/RNA extractors in the large volume urine DNA/RNA extraction systems will into catheter, extractor, go out
Catheter becomes one, while using the female Luer easily connected, plus broken part is provided with catheter is gone out, works as DNA/
After RNA extractors have extracted DNA/RNA, it will go out catheter from broken part and fracture, the main part of DNA/RNA extractors will be left
As check analysis, these structures make operation very simple, also greatly reducing the secondary pollution of DNA/RNA.
In order to achieve the above object, the utility model adopts the following technical solution：Taken out including DNA/RNA extractors, negative pressure
Haustorium, container, its key are：The DNA/RNA extractors include extractor body and vacuum suction connection mouth, wherein carrying
Take the top of device body to be provided with into catheter and coordinate installation with containing urine container；
The lower end of the extractor body is provided with out catheter, this goes out catheter and connects the vacuum pumping by broken part
Connection mouth is inhaled, the vacuum suction connection mouth is connected by dismountable Hermetical connecting structure with negative pressure aspirator；
The negative pressure aspirator aspirates the urine contained in urine container through DNA/RNA extractors；
DNA/RNA extractors are used to adsorb the DNA/RNA in urine；
The container is used to be set with the extractor body, and contains the DNA/RNA isolated in extractor body.
DNA/RNA extractors are used for connection Sheng urine container into catheter, and it is to be extracted for containing to contain urine container
The urine of DNA/RNA, negative pressure aspirator aspirates the urine contained in urine container through DNA/RNA extractors, when urine passes through DNA/
During RNA extractors, the DNA/RNA that the adsorbent that is set in DNA/RNA extractors is adsorbed in urine is used for check analysis.
The utility model is connected true by setting the broken part for being used for fractureing on the outer wall for go out catheter by broken part
Sky suction connection mouth, vacuum suction connection mouth are connected by dismountable Hermetical connecting structure with negative pressure aspirator.Work as DNA/RNA
After extractor has extracted the DNA/RNA in urine, catheter will be gone out and fractureed with vacuum suction connection mouth at broken part, will
Vacuum suction connection mouth is thrown away, and then will be contained urine container and is removed, then extractor body is put into container make further from
The heart separates DNA/RNA.
The broken part is annular groove, the section either triangle, or rectangle of the annular groove, or ladder
Shape, or it is arc-shaped.
Annular groove is easy to fracture, and the section of above-mentioned annular groove was both easy to process, also allows for fractureing.
The Hermetical connecting structure is female Luer.
Female Luer good seal performance, and it is easy to connect.
The female Luer includes female Luer connector and public female Luer, female Luer connector and the vacuum suction connection mouth
Connect or be integrally formed, the public affairs female Luer is connected with the suction chamber of negative pressure aspirator.
Public female Luer is used for the pump orifice for connecting negative pressure aspirator, easy to aspirate urine.Female Luer connector is taken out with vacuum
Connection mouth is inhaled, goes out catheter and is integrally formed, structure is simplified, eliminates connecting leg and seal nipple of the prior art.
The DNA/RNA extractors are connected into catheter with containing urine container by female Luer.
Said structure is easy to DNA/RNA extractors and the sealed connection for containing urine container.
The suction chamber of the negative pressure aspirator is provided with bleeding point, which is connected with pumping after extraction valve by pipeline
Air pump, the bottom of the suction chamber are additionally provided with void urine mouth, which is connected with urination liquid pipe through void urine valve.
The structure of above-mentioned negative pressure aspirator is simple, and urination valve is closed, and extraction valve is opened, and contains the urine in urine container
Enter suction chamber by DNA/RNA extractors under the action of atmospheric pressure, after having aspirated urine, extraction valve is closed, will be arranged
Urine valve is opened, and discharges waste liquid.
The container includes collecting pipe and the centrifugation cylinder that is sleeved on outside collecting pipe, collecting pipe be used to being socketed it is described go out
Catheter.
DNA/RNA extractors are fractureed at broken part, throw away vacuum suction connection mouth, pull down Sheng urine container, will be gone out
Catheter is put into collecting pipe, then collecting pipe is put into and is centrifuged in cylinder, and centrifugation cylinder is put into centrifuge is centrifuged
Separate, the DNA/RNA in DNA/RNA extractors is fallen into collecting pipe under the influence of centrifugal force.
The centrifugation cylinder is provided with support blind hole, and the bottom of the collecting pipe is placed on the bottom of support blind hole.
The bottom of support blind hole supports collecting pipe, is put into rotate when centrifuge is separated and more stablizes.
Remarkable result:The utility model provides a kind of large volume urine DNA/RNA extraction systems, the large volume urine
DNA/RNA extractors in DNA/RNA extraction systems will into catheter, extractor, go out catheter and become one, use at the same time
The female Luer easily connected, plus being provided with broken part on catheter is gone out, after DNA/RNA extractors have extracted DNA/RNA,
It will go out catheter from broken part to fracture, the main part of DNA/RNA extractors will be left as check analysis, these structures make behaviour
Make very simple, also greatly reducing the secondary pollution of DNA/RNA.
Brief description of the drawings
Fig. 1 is the usage state diagram of DNA/RNA extractors and negative pressure aspirator；
Fig. 2 is the structure chart of original DNA/RNA extractors；
Fig. 3 is the usage state diagram of DNA/RNA extractors and the DNA/RNA containers isolated.
Embodiment
The utility model is described in further detail with specific embodiment below in conjunction with the accompanying drawings.
As shown in figures 1 and 3, a kind of large volume urine DNA/RNA extraction systems, including DNA/RNA extractors 1, negative pressure
Aspirator 2, container 3, the DNA/RNA extractors 1 include extractor body and vacuum suction connection mouth 8, wherein extractor
The top of body is provided with into catheter 12 coordinates installation with containing urine container 5；
The lower end of the extractor body is provided with out catheter 11, this goes out catheter 11 and passes through broken part 11a connections institute
Vacuum suction connection mouth 8 is stated, the vacuum suction connection mouth 8 passes through dismountable Hermetical connecting structure and 2 phase of negative pressure aspirator
Even；
The negative pressure aspirator 2 aspirates the urine contained in urine container 5 through DNA/RNA extractors 1；
DNA/RNA extractors 1 are used to adsorb the DNA/RNA in urine；
The container 3 is used to be set with the extractor body, and contains the DNA/RNA isolated in extractor body.
DNA/RNA extractors 1 are used for connection Sheng urine container 5 into catheter, and it is to be extracted for containing to contain urine container 5
The urine of DNA/RNA, negative pressure aspirator aspirate the urine contained in urine container 5 through DNA/RNA extractors 1, when urine is passed through
During DNA/RNA extractors 1, DNA/RNA that the adsorbent that is set in DNA/RNA extractors 1 is adsorbed in urine is used to examining point
Analysis.
The utility model passes through broken part by setting the broken part 11a for being used for fractureing on the outer wall for going out catheter 11
11a connections are sucked by vacuum connection mouth 8, and vacuum suction connection mouth 8 passes through dismountable Hermetical connecting structure and 2 phase of negative pressure aspirator
Even.After DNA/RNA extractors 1 have extracted the DNA/RNA in urine, catheter 11 will be gone out with being sucked by vacuum connection mouth 8 from folding
Fracture at disconnected portion 11a, vacuum suction connection mouth 8 is thrown away, urine container 5 will be contained and removed, then extractor body is put into
Make further centrifuging treatment in container 3.
The broken part 11a is annular groove, the section either triangle, or rectangle of the annular groove, or
It is trapezoidal, or arc-shaped.
Annular groove is easy to fracture, and the section of above-mentioned annular groove was both easy to process, also allows for fractureing.
The Hermetical connecting structure is female Luer 4.
Female Luer good seal performance, and it is easy to connect.
The female Luer 4 includes female Luer connector 41 and public female Luer 42, and female Luer connector 41 is taken out with the vacuum
Inhale connection mouth 8 to connect or be integrally formed, the public affairs female Luer 42 is connected with the suction chamber 21 of negative pressure aspirator 2.
Public female Luer 42 is used for the pump orifice for connecting negative pressure aspirator, easy to aspirate urine.Female Luer connector 41 with it is true
Sky suction connection mouth 8, annular groove, go out catheter 11 and be integrally formed, and simplifies structure, eliminates connecting leg 6 of the prior art
With seal nipple 7.
The DNA/RNA extractors 1 are connected into catheter 12 with containing urine container 5 by female Luer.
Said structure is easy to DNA/RNA extractors 1 and the sealed connection for containing urine container 5.
The suction chamber 21 of the negative pressure aspirator 2 is provided with bleeding point, which is connected after extraction valve 22 by pipeline
Aspiration pump is connected to, aspiration pump omits be not shown in Fig. 1, and the bottom of the suction chamber 21 is additionally provided with void urine mouth, the urination
Liquid mouth is connected with urination liquid pipe through void urine valve 23.
The structure of above-mentioned negative pressure aspirator 2 is simple, and urination valve 23 is closed, and extraction valve 22 is opened, and contains in urine container 5
Urine under the action of atmospheric pressure by DNA/RNA extractors 1 enter suction chamber 21, after having aspirated urine, by extraction valve
22 close, and urination valve 23 is opened, and discharge waste liquid.
The container 3 includes collecting pipe 31 and the centrifugation cylinder 32 being sleeved on outside collecting pipe 31, collecting pipe 31 are used for
Go out catheter 11 described in socket.
DNA/RNA extractors 1 are fractureed at the 11a of broken part, vacuum suction connection mouth 8 is thrown away, pulls down Sheng urine container
5, catheter 11 will be gone out and be put into collecting pipe 31, then collecting pipe 31 is put into and is centrifuged in cylinder 32, cylinder 32 will be centrifuged and put
Enter centrifuge to be centrifuged, the DNA/RNA in DNA/RNA extractors 1 is fallen into collecting pipe 31 under the influence of centrifugal force.
The centrifugation cylinder 32 is provided with support blind hole, and the bottom of the collecting pipe 31 is placed on the bottom of support blind hole
On.
The bottom of support blind hole supports collecting pipe 31, is put into rotate when centrifuge is separated and more stablizes.
The collecting pipe 31 includes the cylindrical portion of upper end and the taper portion of lower end, the big end of taper portion and the bottom of cylindrical portion
It is connected, the collecting pipe 31 is internally provided with accommodating cavity, houses the open top of cavity, which is sleeved on out urine
The outside of pipe 11 is used to collect DNA/RNA.
Since the DNA/RNA fallen down is seldom, said structure is easy to concentrate on DNA/RNA the taper portion of collecting pipe 31
Bottom.
As shown in Fig. 2, Fig. 2 is the structure chart of original DNA/RNA extractors, the catheter that goes out of former DNA/RNA extractors 9 passes through
Connecting leg 6 connects an independent sealed connection connector 7, its is complicated, and manufacture is difficult, and part is more, it is not easy to ensure sealing,
Easily cause secondary pollution.
The operation principle of the utility model is：First by DNA/RNA extractors 1 into catheter 12 and contain 5 phase of urine container
Connection, then by with going out the integrally formed female Luer connector 41 of catheter 11 with being installed on the pump orifice of negative pressure aspirator 2
Public female Luer 42 is connected, and the urine of DNA/RNA to be extracted is added in urine container 5 is contained.
The urination valve 23 of negative pressure aspirator 2 is closed, extraction valve 22 is opened, aspiration pump pumping is started, contains urine container 5
In urine under the action of atmospheric pressure by DNA/RNA extractors 1 enter suction chamber 21, after having aspirated urine, will be evacuated
Valve 22 is closed.
Catheter 11 will be gone out at the 11a of broken part to fracture, urine container 5 will be contained from into removing on catheter 12, will be extracted
Device body is left as further centrifuging treatment.
Contaminated vacuum suction connection mouth 8 is removed with female Luer connector 41 from public female Luer 42 and is thrown away.
Urination valve 23 is opened, discharges waste liquid.
The catheter 11 that goes out of extractor body is socketed with collecting pipe 31 again, collecting pipe 31 is put together with extractor body
In the support blind hole for entering to centrifuge cylinder 32, then by centrifuge cylinder 32 be put into centrifuge, start centrifuge, extractor body
DNA/RNA on interior adsorbent is fallen into collecting pipe 31 under powerful centrifugal force effect, completes the extraction of DNA/RNA.
Finally it should be noted that be：Listed above is only specific embodiment of the utility model, certain this area
The utility model can be modified by technical staff and modification, if these modifications and variations belong to the utility model right and want
Ask and its equivalent technologies within the scope of, be considered as the scope of protection of the utility model.
Claims (8)
1. a kind of large volume urine DNA/RNA extraction systems, including DNA/RNA extractors (1), negative pressure aspirator (2), container
(3), it is characterised in that：The DNA/RNA extractors (1) include extractor body and vacuum suction connection mouth (8), wherein extracting
The top of device body is provided with into catheter (12) coordinates installation with containing urine container (5)；
The lower end of the extractor body is provided with out catheter (11), this goes out catheter (11) and is connected by broken part (11a)
The vacuum suction connection mouth (8), the vacuum suction connection mouth (8) pass through dismountable Hermetical connecting structure and negative-pressure ward
Device (2) is connected；
The negative pressure aspirator (2) aspirates the urine contained in urine container (5) through DNA/RNA extractors (1)；
DNA/RNA extractors (1) are used to adsorb the DNA/RNA in urine；
The container (3) is used to be set with the extractor body, and contains the DNA/RNA isolated in extractor body.
2. large volume urine DNA/RNA extraction systems according to claim 1, it is characterised in that：The broken part (11a)
For annular groove.
3. large volume urine DNA/RNA extraction systems according to claim 2, it is characterised in that：It is described to be tightly connected knot
Structure is female Luer (4).
4. large volume urine DNA/RNA extraction systems according to claim 3, it is characterised in that：The female Luer (4)
Including female Luer connector (41) and public female Luer (42), female Luer connector (41) is connected with the vacuum suction connection mouth (8)
Or be integrally formed, the public affairs female Luer (42) is connected with the suction chamber (21) of negative pressure aspirator (2).
5. large volume urine DNA/RNA extraction systems according to claim 1, it is characterised in that：The DNA/RNA extractions
Device (1) is connected into catheter (12) with containing urine container (5) by female Luer.
6. large volume urine DNA/RNA extraction systems according to claim 1, it is characterised in that：The negative pressure aspirator
(2) suction chamber (21) is provided with bleeding point, which is connected with aspiration pump, the pumping after extraction valve (22) by pipeline
The bottom for inhaling chamber (21) is additionally provided with void urine mouth, which is connected with urination liquid pipe through void urine valve (23).
7. large volume urine DNA/RNA extraction systems according to claim 1, it is characterised in that：Container (3) bag
Include collecting pipe (31) and be sleeved on the centrifugation cylinder (32) of collecting pipe (31) outside, collecting pipe (31) be used to being socketed it is described go out urine
Manage (11).
8. large volume urine DNA/RNA extraction systems according to claim 7, it is characterised in that：The centrifugation cylinder
(32) support blind hole is provided with, the bottom of the collecting pipe (31) is placed on the bottom of support blind hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721062752.6U CN207227399U (en) | 2017-08-24 | 2017-08-24 | A kind of large volume urine DNA/RNA extraction systems |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721062752.6U CN207227399U (en) | 2017-08-24 | 2017-08-24 | A kind of large volume urine DNA/RNA extraction systems |
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| Publication Number | Publication Date |
|---|---|
| CN207227399U true CN207227399U (en) | 2018-04-13 |
Family
ID=61857686
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721062752.6U Expired - Fee Related CN207227399U (en) | 2017-08-24 | 2017-08-24 | A kind of large volume urine DNA/RNA extraction systems |
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| Country | Link |
|---|---|
| CN (1) | CN207227399U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110656026A (en) * | 2019-09-29 | 2020-01-07 | 成都市第五人民医院 | Automatic urine DNA purification device |
-
2017
- 2017-08-24 CN CN201721062752.6U patent/CN207227399U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110656026A (en) * | 2019-09-29 | 2020-01-07 | 成都市第五人民医院 | Automatic urine DNA purification device |
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| GR01 | Patent grant | ||
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180413 |