CN211665049U

CN211665049U - High-efficient instrument of obtaining single cell suspension

Info

Publication number: CN211665049U
Application number: CN201921658293.7U
Authority: CN
Inventors: 何方; 贾慧琳; 刘涛
Original assignee: Affiliated Hospital of Zunyi Medical University
Current assignee: Affiliated Hospital of Zunyi Medical University
Priority date: 2019-09-30
Filing date: 2019-09-30
Publication date: 2020-10-13
Anticipated expiration: 2029-09-30

Abstract

The utility model discloses an instrument of unicellular suspension is acquireed to high efficiency, the drill comprises a tool body, this internal cavity that is equipped with of instrument, be equipped with the nylon filter screen in the cavity, the both ends of instrument body all are equipped with the screw thread end, be equipped with two aspirating holes on the lateral wall of instrument body, one of them the aspirating hole is located the top of nylon filter screen, another the aspirating hole is located the below of nylon filter screen. The utility model discloses specially adapted clinical grade stem cell's extraction can make things convenient for effectual unicellular suspension who obtains high biological activity fast to guarantee primary umbilical cord or amnion mesenchymal stem cell quality.

Description

High-efficient instrument of obtaining single cell suspension

Technical Field

The utility model relates to a medical science biotechnology field especially relates to a high-efficient instrument of acquireing unicellular suspension.

Background

For biomedical researchers, highly active cells are essential for in vivo and in vitro experiments and clinical transformation applications, and according to specific experimental needs, single cell suspensions are usually prepared from various samples. Medical detection and research such as flow cytometry analysis or magnetic bead sorting also need to remove tissue fragments and cell aggregates in a sample so as to obtain a single cell suspension.

For how to separate single cell suspension from tissue mass suspension after enzyme digestion, most of the existing methods utilize a screen with a certain aperture to filter the cell suspension by means of gravity, and the gravity filtering method has more cells adhered and remained on the screen, so that the time required by single cell extract is long, the activity of primary cells is reduced, and the acquisition rate of the single cell suspension is not high, thereby influencing the sterile extraction and purification of the primary cells of amnion mesenchymal stem cells from placenta amnion and umbilical cord mesenchymal stem cells from Wharton jelly.

SUMMERY OF THE UTILITY MODEL

The purpose of the utility model is to solve the shortcoming that exists among the prior art, if: most of the existing methods utilize a screen with a certain aperture to filter cell suspension by means of gravity, and the gravity filtering method has more cells adhered and remained on the screen, so that the single cell extracting solution needs long time, the activity of primary cells is reduced, and the acquisition rate of the single cell suspension is not high, thereby influencing the sterile extraction and purification of the primary cells of the amniotic mesenchymal stem cells from the placenta amnion and the umbilical cord mesenchymal stem cells from the Wharton's jelly.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a tool for efficiently obtaining single cell suspension, which comprises a tool body, wherein a cavity is arranged in the tool body, a nylon filter screen is arranged in the cavity, the inner walls of the two ends of the tool body are both provided with threaded ends, the outer side wall of the tool body is provided with two air exhaust holes, one of the air suction holes is positioned above the nylon filter screen, the other air suction hole is positioned below the nylon filter screen, an air exhaust pipe communicated with an air exhaust hole is fixedly connected on the outer side wall of the tool body, a thread groove is arranged on the inner side wall of the air exhaust hole close to one end of the cavity, a filter membrane is arranged in the thread groove, a thread sleeve is connected in the thread groove in a threaded manner, a thread is arranged at one end of the exhaust tube far away from the tool body, the air exhaust pipe is connected with a pipe fitting in a threaded mode, one end, far away from the air exhaust pipe, of the pipe fitting is provided with a groove, and a connecting pipe is sleeved on the groove.

Preferably, the thread end is provided with a rubber backing ring, and the thread groove and the thread can be butted with a conventional centrifugal tube.

Preferably, the cross section of the nylon filter screen is circular, the nylon filter screen is fixed in the middle of the cavity and divides the cavity into two containing cavities.

Preferably, a gasket is clamped between the filter membrane and the threaded pipe sleeve, and anti-skid stripes are arranged at one end, far away from the gasket, of the threaded pipe sleeve.

Preferably, the tool body is made of a transparent polypropylene material, and the filter membrane is made of a polyvinylidene fluoride membrane material.

Preferably, the connecting pipe is a PU steel wire hose.

The utility model has the advantages that:

1. compared with the traditional method of filtering the single cell suspension by the filter screen under the action of gravity, the tool body can effectively shorten the time required by the extraction of the single cell suspension, thereby effectively improving the activity of primary cells.

2. The negative pressure extraction method adopted by the tool body can comprehensively filter the cell liquid drops attached to the nylon filter screen and the undigested tissue block into the centrifugal tube below the nylon filter screen to the maximum extent, so that the acquisition rate of the single cell suspension is improved.

3. When the tool body is operated, the accommodating cavity inside the tool body and the centrifugal tube form a relatively closed space, and the air pumping hole part communicated with the outside is provided with a 0.22 or 0.3 mu m filter membrane for separation, so that microbial pollution caused by misoperation and the like can be effectively controlled.

In a word, the tool body is particularly suitable for extracting clinical-grade stem cells, and can quickly, conveniently and effectively obtain single cell suspension with high biological activity, so that the quality of primary umbilical cord or amniotic mesenchymal stem cells is ensured.

Drawings

FIG. 1 is a schematic diagram of the front structure of a tool for efficiently obtaining single cell suspension according to the present invention;

FIG. 2 is a schematic diagram of the internal structure of a tool for efficiently obtaining a single cell suspension according to the present invention;

fig. 3 is an enlarged view of the structure a in fig. 2.

In the figure: 1 tool body, 2 cavities, 3 nylon filter screens, 4 threaded ends, 5 rubber backing rings, 6 air extraction holes, 7 air extraction pipes, 8 threaded grooves, 9 filter membranes, 10 gaskets, 11 threaded pipe sleeves, 12 pipe fittings, 13 grooves and 14 connecting pipes.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

Referring to fig. 1-3, a high-efficient instrument of obtaining unicellular suspension, including tool body 1, be equipped with cavity 2 in the tool body 1, be equipped with nylon filter screen 3 in the cavity 2, nylon filter screen 3 can be divided into a plurality of specifications of 40, 70 or 100 mu m aperture, the cross section of nylon filter screen 3 is circular, be fixed in the middle part of cavity 2 in the nylon filter screen 3, nylon filter screen 3 separates cavity 2 into two and holds the chamber, accomplish the aseptic extraction and the purification of unicellular suspension with the help of nylon filter screen 3, the both ends inner wall of tool body 1 all is equipped with threaded end 4, be used for with the Falcon of 15ml, 50ml or 175ml that conventionally use, the inner wall of^TMThe centrifuging tube screw thread matches, and the accessible is rotatory to be in close butt joint with the centrifuging tube, guarantees that the inside liquid of centrifuging tube can not spill over, makes and holds chamber and centrifuging tube formationThe whole body and the whole inner part are in a relatively closed state.

Threaded end 4 is equipped with rubber backing ring 5, when the tight butt joint of threaded end 4 and centrifuging tube at instrument body 1 with the help of its both ends, the leakproofness of being connected between instrument body 1 and the centrifuging tube can be ensured to rubber backing ring 5, thereby prevent to acquire the in-process of unicellular suspension and appear polluting because of outside microorganism gets into, be equipped with two aspirating holes 6 on the lateral wall of instrument body 1, one of them aspirating hole 6 is located the top of nylon filter screen 3, another aspirating hole 6 is located the below of nylon filter screen 3, fixedly connected with intercommunication aspirating hole 6's exhaust tube 7 on instrument body 1's the lateral wall.

The exhaust tube 7 can match with the pipeline of the waste liquid suction pump in cell room, the function of taking out air of below when guaranteeing the instrument body 1 to use, and two exhaust tubes 7 can use at random, have the top function of admitting air or below and bleeding concurrently, be equipped with thread groove 8 on the inside wall that exhaust hole 6 is close to 2 one end of cavity, be equipped with filter membrane 9 in the thread groove 8, instrument body 1 is made by transparent polypropylene material, filter membrane 9 is made by polyvinylidene fluoride membrane material, filter membrane 9 is located inside exhaust hole 6, the aperture is 0.22 or 0.3 mu m, be used for keeping apart and hold chamber and external environment, can guarantee that two of this instrument body 1 hold the inside suspension in chamber and centrifuging tube and be in aseptic condition, prevent that outside microorganism from getting into inside pollution suspension, can prevent overflowing of liquid in the use simultaneously.

8 threaded connection in thread groove has threaded pipe box 11, press from both sides between filter membrane 9 and the threaded pipe box 11 and be equipped with packing ring 10, threaded pipe box 11 is kept away from one of packing ring 10 and is served and be equipped with anti-skidding strip, filter membrane 9 is convenient for install and change, packing ring 10 can avoid twisting the produced wearing and tearing because of direct contact between threaded pipe box 11 and the filter membrane 9 when installing threaded pipe box 11, exhaust tube 7 keeps away from one of instrument body 1 and serves and be equipped with the screw thread, threaded connection has pipe fitting 12 on the exhaust tube 7, pipe fitting 12 keeps away from one of exhaust tube 7 and serves and be equipped with recess 13, the cover is equipped with connecting pipe 14 on the recess 13, connecting pipe 14 adopts PU steel wire hose, be difficult to warp under the.

In the utility model, when the user uses the device, the amnion or the umbilical cord mesenchyme is usedFor the stem cell single cell suspension extraction, for example, in a sterile environment, 15ml, 50ml or 175ml Falcon was selected^TMPerforming primary extraction operation on umbilical cord mesenchymal stem cells or amniotic mesenchymal stem cells by an empty centrifugal tube according to an enzyme digestion method, namely completely digesting umbilical cord Wharton's jelly fragments or placental goat membranes in the centrifugal tube by pancreatin or collagenase according to a scheme according to the steps;

selecting an aseptic tool body 1 with corresponding specification, aligning a thread end 4 of the tool body 1 with a thread of a centrifugal tube filled with digestive suspension, rotationally screwing, aligning the other thread end 4 of the tool body 1 with the other hollow centrifugal tube, rotationally screwing, positioning the hollow centrifugal tube below, positioning the centrifugal tube filled with digestive juice above, allowing digestive juice of Wharton's jelly fragments or placenta caplets to enter an upper accommodating cavity of a cavity 2 in the tool body 1, linking a pipeline of a suction pump with an air suction hole 6 below the hollow centrifugal tube corresponding to the hollow centrifugal tube, starting the air suction pump to slowly pump air, allowing single-cell suspension in the upper centrifugal tube to flow into the lower hollow centrifugal tube under the negative pressure action of the lower accommodating cavity, and completing aseptic extraction and purification of the target single-cell suspension by means of a nylon filter screen 3, wherein the tool body 1 is particularly suitable for extracting clinical-grade dry cells, the method can quickly, conveniently and effectively obtain the single cell suspension with high biological activity, thereby ensuring the quality of the primary umbilical cord or amniotic mesenchymal stem cells.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. The tool for efficiently obtaining the single cell suspension comprises a tool body (1) and is characterized in that a cavity (2) is arranged in the tool body (1), a nylon filter screen (3) is arranged in the cavity (2), threaded ends (4) are arranged on the inner walls of two ends of the tool body (1), two air exhaust holes (6) are formed in the outer side wall of the tool body (1), one air exhaust hole (6) is located above the nylon filter screen (3), the other air exhaust hole (6) is located below the nylon filter screen (3), an air exhaust pipe (7) communicated with the air exhaust holes (6) is fixedly connected to the outer side wall of the tool body (1), a thread groove (8) is formed in the inner side wall of one end, close to the cavity (2), of the air exhaust hole (6), a filter membrane (9) is arranged in the thread groove (8), and a thread sleeve (11) is connected to the thread groove (8), one end of the exhaust tube (7) far away from the tool body (1) is provided with a thread, the exhaust tube (7) is connected with a pipe fitting (12) through the thread, one end of the pipe fitting (12) far away from the exhaust tube (7) is provided with a groove (13), and the groove (13) is sleeved with a connecting pipe (14).

2. The tool for obtaining single cell suspension with high efficiency as claimed in claim 1, wherein the threaded end (4) is provided with a rubber backing ring (5), and the thread of the threaded end (4) can be butted with a conventional centrifuge tube.

3. The tool for efficiently obtaining the single cell suspension as claimed in claim 1, wherein the cross section of the nylon filter screen (3) is circular, the nylon filter screen (3) is fixed in the middle of the cavity (2), and the nylon filter screen (3) divides the cavity (2) into two containing cavities.

4. The tool for obtaining a single cell suspension with high efficiency as claimed in claim 1, wherein a gasket (10) is interposed between the filter membrane (9) and the threaded pipe sleeve (11), and an anti-slip stripe is arranged on one end of the threaded pipe sleeve (11) far away from the gasket (10).

5. The tool for obtaining single cell suspension with high efficiency as claimed in claim 1, wherein the tool body (1) is made of transparent polypropylene material, and the filter membrane (9) is made of polyvinylidene fluoride membrane material.

6. The tool for obtaining single cell suspension efficiently as claimed in claim 1, wherein the connecting tube (14) is made of PU steel wire hose.