CN205933795U - Manual separation single cell's of under a microscope device - Google Patents
Manual separation single cell's of under a microscope device Download PDFInfo
- Publication number
- CN205933795U CN205933795U CN201620848287.8U CN201620848287U CN205933795U CN 205933795 U CN205933795 U CN 205933795U CN 201620848287 U CN201620848287 U CN 201620848287U CN 205933795 U CN205933795 U CN 205933795U
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- Prior art keywords
- pipette
- connector
- pipettor
- range
- cell
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- 238000000926 separation method Methods 0.000 title abstract description 8
- 239000011521 glass Substances 0.000 claims description 29
- 210000005087 mononuclear cell Anatomy 0.000 claims description 12
- 239000010409 thin film Substances 0.000 claims description 3
- 210000004027 cell Anatomy 0.000 description 23
- 238000005516 engineering process Methods 0.000 description 10
- 239000007788 liquid Substances 0.000 description 10
- 238000000034 method Methods 0.000 description 9
- 238000010586 diagram Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000006285 cell suspension Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 230000003321 amplification Effects 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 101100433727 Caenorhabditis elegans got-1.2 gene Proteins 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 239000010408 film Substances 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 229910002804 graphite Inorganic materials 0.000 description 1
- 239000010439 graphite Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000001990 intravenous administration Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000005060 rubber Substances 0.000 description 1
- 238000005204 segregation Methods 0.000 description 1
- 239000008174 sterile solution Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model relates to a manual separation single cell's of under a microscope device, it is constituteed by inhaling measurer, pipette, cavity picking needle. Measurer, pipette, cavity picking needle are inhaled including what connect gradually to the device, wherein, cavity picking needle is used for absorbing single cell by the setting, it is used for control by the setting to inhale the measurer cavity picking needle. Use the utility model discloses a separation single cell's device can the simply effectual single cell that acquires.
Description
Technical field
This utility model be related to biological technical field and in particular under a kind of microscope manual isolated mononuclear cell dress
Put.
Background technology
Single celled research is one of life science research direction with fastest developing speed, and stable unicellular separation and
Nucleic acid amplification technologies are the most crucial support technologies in this field, and the imaginative power of scientist can not be subject to for single celled acquisition
The limitation of technology, is advanced the research of tumor, immunity and developmental biology to obtain in real individual cell level to a great extent
Obtain progress greatly.
Current unicellular separation method includes：（1）Fluorescence flow cytometry technology partition method：The method can obtain single
Cell, but higher to the performance requirement of cell instrument, and instrument price is high；（2）Laser microprobe dating art：Can divide from tissue
From individual cells, but it is not suitable for cell culture and amplification, need operator skillfully to master a skill；（3）Micro- manipulation instrument：Adopt
Drawn unicellular from single cell suspension with micro- manipulation technology, but high cost, complex operation；（4）Manual machinery partition method,
It is directly separated cell under inverted microscope, but easy damaging cells；（5）Make unicellular segregation apparatuss by oneself：Including breather, glass
Glass pipe, during use, oral area adjusts air pressure supply draw power, and control device draws individual cells, and the method oral area is directly drawn,
There are great security risk and experimental pollution possible, test after finishing simultaneously, the hollow ventilating pipe passing through as air-flow（Generally
Hose）Typically need to reclaim and reuse, on the one hand be likely to result in pollution, on the other hand need equipment is carried out disinfection, operation
Loaded down with trivial details.
In view of the drawbacks described above of prior art, those skilled in the art are devoted to a kind of simple, safety of research and development, easily operate
Isolated mononuclear cell device.
Content of the invention
In order to solve the problems, such as that in prior art, individual cells separation is difficult, equipment requirements are high, technology dependence is strong, this reality
With the new device providing the isolated mononuclear cell that a kind of structure simply, safely, easily manipulates, carried out single using this device
The separation of cell.
Based on object above, this utility model provides a kind of device of manual isolated mononuclear cell under microscope.
1st, under a kind of microscope manual isolated mononuclear cell device, wherein,
Described device is made up of pipette device, pipette and hollow picking pin；
Described pipette device includes range adjustment portion, Handheld Division and suction nozzle；
Described pipette two ends are respectively provided with the first connector and the second connector；
Described pipette device and pipette are attached by the first connector, and described hollow picking pin and pipette pass through the
Two connectors are attached.
2nd, the device according to item 1, wherein, described be connected as be socketed.
3rd, the device according to item 1 or 2 any one, wherein, the range of described pipette device is indefinite range, fixing range
Or range-adjustable.
4th, the device according to any one of item 1 ~ 3, wherein, described pipette device is pipettor.
5th, the device according to item 4, wherein, the specification of described pipettor is 10 ~ 100 μ L.
6th, the device according to any one of item 1 ~ 5, wherein, described pipette is venoclysis needle.
7th, the device according to any one of item 1 ~ 6, wherein, described hollow picking pin is glass capillary.
8th, the device according to item 2, wherein, the junction of described socket is closed using thin film or seal with wax.
9th, the device according to any one of item 1 ~ 8, wherein, described first connector is arranged on the oral area of suction nozzle, described
Second connector is arranged on one end of hollow picking pin, and described pipette two ends arrange or are not provided with or arrange first at one end even
Fitting and/or the second connector.
Compared with prior art, this utility model drastically increases the single celled accuracy of acquisition, simple to operate, and drops
Low application risk, can directly be assembled.
Brief description
Fig. 1 is the overall structure diagram of the device of isolated mononuclear cell of the present utility model；
Fig. 2 is pipettor schematic diagram；
Fig. 3 is venoclysis needle schematic diagram；
Fig. 4 is glass capillary schematic diagram；
In figure：1- capillary glass, 2- venoclysis needle, 3- pipettor, 31- Handheld Division, 32- adjustment portion, 33- suction nozzle, 4-
Second connector, 5- first connector.
Specific embodiment
Unless otherwise defined, the technology people of all technology as used herein and scientific terminology and the technical field of the invention
The implication that member is generally understood that is identical.Although any method similar or suitable to method described herein and material and material
Can be used for carrying out or detect the present invention.Preferred embodiment described herein is merely to illustrate and explains this utility model, but
It is not used to limit this utility model.
Pipette device, pipette and hollow that the device of the isolated mononuclear cell that this utility model provides includes being sequentially connected are chosen
Take pin.This utility model does not particularly limit to the connected mode of described pipette device, described pipette and described hollow picking pin
System, as long as facilitate the connected mode of described pipette device, described pipette and described hollow picking pin to may be used to organize cost
The device of the isolated mononuclear cell of utility model, and be applied to single celled lock out operation.Described connected mode can be selected from
But be not limited to be socketed, snap connection, familiar to those skilled in the art those such as threaded, be preferably socketed.Described pipette
Device, described pipette and described hollow picking pin can be distinguished in junction or all arrange containment member.Described containment member
Sealing gasket or diaphragm seal can be selected from, but not limited to,.The form of described sealing gasket can be selected from, but not limited to, spirotallic gasket or ring-type
Pad.The material of described sealing gasket can be selected from, but not limited to, familiar to those skilled in the art that such as metal, rubber, graphite
A bit.The material of described diaphragm seal can be selected from, but not limited to, familiar to those skilled in the art those such as thin film or waterproof wax,
Preferred film.
Described pipette device can adopt familiar to those skilled in the art those, and for example, pipette device adopts pipettor, note
Emitter, glue head dropper etc..Described pipettor can be selected from, but not limited to, Manual liquid transfering device, automatic pipettor etc..Described pipettor
Range can be selected from, but not limited to, 1 ~ 1000 μ L, preferably 5 ~ 500 μ L, more preferably 10 ~ 100 μ L.
Described pipette can adopt familiar to those skilled in the art those, and for example, venoclysis needle, two ends carry
Sebific duct of clamp etc., described intravenous needle can be selected from, but not limited to, 5.5 ~ No. 8, preferably 6 ~ No. 7.
This utility model has no particular limits to the suction nozzle external diameter of described pipette device and the internal diameter of described second connector,
As long as the suction nozzle facilitating described pipette device may be used to form of the present utility model separation with the connection of described second connector
The device of individual cells, and be applied to single celled lock out operation.
Described hollow picking pin can adopt familiar to those skilled in the art those, for example, glass capillary etc..Institute
The internal diameter stating hollow picking pin can be selected from, but not limited to, less than 150 μm, preferably less than 100 μm.
Embodiment 1
The device of the isolated mononuclear cell of the present embodiment includes glass capillary 1, venoclysis needle 2 and the shifting being sequentially connected
Liquid device 3, as shown in Figure 1.Glass capillary 1 is arranged for drawing unicellular；Venoclysis needle 2（The present embodiment is defeated using No. 7
Liquid pin）It is arranged between glass capillary 1 and pipettor 3 and be used for connecting glass capillary 1 and pipettor 3；Pipettor 3 is set
Put for controlling glass capillary 1.
In the present embodiment, the internal diameter of the glass capillary 1 of employing is 100 μm.Using pipette device 3 include range adjustment portion
31st, Handheld Division 32 and suction nozzle 33, the range of pipettor 3 is 10 ~ 100 μ L（Purchased from Eppendorf company）.Using venous transfusion
The specification of pin 2 is 0.7mm, and the two ends of venoclysis needle 2 are respectively the first connector 5 and the second connector 4.Glass capillary 1
Connected by the first connector 5 and venoclysis needle 2, one section of glass capillary 1 is socketed in the inside of the first connector 5, set
Sealed membrane is adopted to be wound around the outer of the first connector 5 after connecing, the interface of closing the first connector 5 and glass capillary 1；Pipette
Device 3 passes through suction nozzle 33 and the second connector 4 is directly connected to venoclysis needle 2, and suction nozzle 33 is socketed in the interior of the second connector 4
Portion.
The present embodiment provide isolated mononuclear cell device can as needed detached difference cell size, change
The glass capillary of different inner diameters, venoclysis needle 2 and pipettor 3 can be reused after cleaning.
Before this utility model device, need the air-tightness of detection means：The glass capillary 1 that finishing device is assembled
Oral area be placed in water, the range adjustment portion of slow pressing pipettor 3 to the first gear, whether observe the oral area of thin glass tube 1
There is bubble continuously to get, and no liquid siphon blows back into collection tube.Journey adjustment portion 31 if there are bubble synchronization pipettor 3 operates
Continuously get, and gas is got rear no liquid siphon and flow backwards, then explanation device air-tightness is normal, can be used for unicellular acquisition in fact
Test.Produce without bubble, bubble is got asynchronous with pipettor 3, or gets liquid after gas and produces and flow backwards, then illustrates close
Whether Feng Buyan, need to check the interface of the interface of the first connector 5 and glass capillary 1 and/or the second connector 4 and suction nozzle 33
Connect normal and detect again, such as detect still problematic again, then need the interface to the first connector 5 and glass capillary 1 and/
Or second the interface of connector 4 and suction nozzle 33 re-start assembling and encapsulation process.
Using method：
Confirm that field of microscope inner cell quantity is unlikely excessive before picking cell（It is advisable with 1 ~ 100）, prevent interference single
The absorption of cell.
1. first device air good for air-leakage test is drained into the first rest point（I.e. full scale shelves）：During this, will
1 point of oral area of glass capillary inserts in ready PBS sterile solution, and the range adjustment portion 31 to the first of pressing pipettor 3 is stopped
Point, additional air is discharged.
2. keep the gear of the range adjustment portion 31 of pipettor 3 to be fixed on the first rest point position, glass capillary 1 is delayed
Slow-speed moves in insertion cell suspension, and glass capillary 1 is directed at a cell by glass capillary 1 and cell under viewing microscope,
Then lift the adjustment portion 31 of pipettor 3, observation of cell is inhaled in glass capillary 1, during keep pipettor 3 regulation
Portion 31 slowly lifts, it is to avoid the amount of lifting is excessive, is drawn onto multiple cells or excessive liquid.
3. remove inhaling the glass capillary 1 having cell from cell suspension, insert the culture fluid that need to inoculate or containing molten
In the collecting pipe of liquid, cell drop is squeezed in liquid.This process is to ensure that cell is fully got, can be by the range of pipettor 3
The gear of adjustment portion 31 is pushed into the second rest point（Empty shelves）, liquid is fully got.
4. keep the range adjustment portion 31 of pipettor 3 under existing pressed state, the glass capillary after cell will be got
1 takes out from solution, then separates the junction of the suction nozzle 33 of pipettor 3 and the second connector 4 of transfusion needle 2, releases afterwards
Put the range adjustment portion 31 of pipettor 3, fully pressure in release whole device.Next reconnect the suction nozzle 33 of pipettor 3
With the interface of the second connector 4 of venoclysis needle 2, and again by glass capillary）In insertion PBS, the amount of pressing pipettor 3
Journey adjustment portion 31 is pressed into the first rest point exclusion additional air（Detect air-tightness simultaneously）, draw for next cell.
Above example only in order to the technical solution of the utility model to be described and unrestricted, although with reference to preferred embodiment pair
This utility model has been described in detail, it will be understood by those within the art that, can be to technology of the present utility model
Scheme is modified or equivalent, without deviating from the spirit and scope of technical solutions of the utility model.
Claims (10)
1. under a kind of microscope manual isolated mononuclear cell device it is characterised in that
Described device is made up of pipette device, pipette and hollow picking pin；
Described pipette device includes range adjustment portion, Handheld Division and suction nozzle；
Described pipette two ends are respectively provided with the first connector and the second connector；
Described pipette device and pipette are attached by the first connector, and described hollow picking pin and pipette pass through second even
Fitting is attached.
2. device according to claim 1 is it is characterised in that described be connected as being socketed.
3. device according to claim 2 is it is characterised in that the junction of described socket is closed using thin film or seal with wax.
4. device according to claim 1 is it is characterised in that the range of described pipette device is indefinite range, fixes range
Or range-adjustable.
5. device according to claim 1 is it is characterised in that described pipette device is pipettor.
6. device according to claim 5 is it is characterised in that the range of described pipettor is 10 ~ 100 μ L.
7. device according to claim 1 is it is characterised in that described pipette is venoclysis needle.
8. device according to claim 1 is it is characterised in that the internal diameter of described hollow picking pin is below 100 μm.
9. device according to claim 1 is it is characterised in that described hollow picking pin is glass capillary.
10. the device according to any one of claim 1-9 is it is characterised in that described first connector is arranged on described suction
The oral area of mouth, described second connector is arranged on one end of described hollow picking pin, and described pipette two ends arrange or are not provided with
Or the first connector and/or the second connector are set at one end.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521121540 | 2015-12-31 | ||
| CN2015211215401 | 2015-12-31 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205933795U true CN205933795U (en) | 2017-02-08 |
Family
ID=57921844
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620848287.8U Active CN205933795U (en) | 2015-12-31 | 2016-08-08 | Manual separation single cell's of under a microscope device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205933795U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113403431A (en) * | 2021-06-30 | 2021-09-17 | 东莞太力生物工程有限公司 | Cell liquid taking control method, device, equipment and storage medium based on robot |
-
2016
- 2016-08-08 CN CN201620848287.8U patent/CN205933795U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113403431A (en) * | 2021-06-30 | 2021-09-17 | 东莞太力生物工程有限公司 | Cell liquid taking control method, device, equipment and storage medium based on robot |
| CN113403431B (en) * | 2021-06-30 | 2022-08-30 | 深圳太力生物技术有限责任公司 | Cell liquid taking control method, device, equipment and storage medium based on robot |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20171225 Address after: 100176 Beijing City, Daxing District branch of Beijing economic and Technological Development Zone Street 88 Hospital No. 8 Building 2 unit 701 room Co-patentee after: ZHEJIANG ANNOROAD BIO-TECHNOLOGY Co.,Ltd. Patentee after: ANNOROAD GENE TECHNOLOGY (BEIJING) Co.,Ltd. Co-patentee after: ANNOROAD (YIWU) MEDICAL INSPECTION CO.,LTD. Address before: 100176 Beijing City, Daxing District branch of Beijing economic and Technological Development Zone Street 88 Hospital No. 8 Building 2 unit 701 room Patentee before: ANNOROAD GENE TECHNOLOGY (BEIJING) Co.,Ltd. |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240416 Address after: Room 101 and Room 201, unit 2, building 8, yard 88, Kechuang 6th Street, Daxing District, Beijing 100176 Patentee after: BEIJING ANNOROAD MEDICAL LABORATORY Co.,Ltd. Country or region after: China Patentee after: ANNOROAD GENE TECHNOLOGY (BEIJING) Co.,Ltd. Address before: Room 701, unit 2, building 8, yard 88, Kechuang 6th Street, Daxing District, Beijing 100176 Patentee before: ANNOROAD GENE TECHNOLOGY (BEIJING) Co.,Ltd. Country or region before: China Patentee before: ZHEJIANG ANNOROAD BIO-TECHNOLOGY Co.,Ltd. Patentee before: ANNOROAD (YIWU) MEDICAL INSPECTION CO.,LTD. |