CN215593064U

CN215593064U - Centrifuging tube device of quickly separating cells

Info

Publication number: CN215593064U
Application number: CN202120043706.1U
Authority: CN
Inventors: 张鲁; 王翔宇; 郭小勇
Original assignee: Guangzhou Mingyutai Biotechnology Co ltd
Current assignee: Guangzhou Mingyutai Biotechnology Co ltd
Priority date: 2021-01-07
Filing date: 2021-01-07
Publication date: 2022-01-21
Anticipated expiration: 2031-01-07

Abstract

The application discloses a centrifugal tube device for quickly separating cells, which comprises a cap, a first tube body, a second tube body and a suction tube; wherein the cap is in threaded connection with the first pipe body; the bottom of the first pipe body is fixedly connected with a second pipe body; the bottom of the second pipe body is of a conical structure; the straw penetrates through the cap, and the first pipe body is communicated with the bottom of the second pipe body. The cell screen is provided with a central small hole, and the cells precipitated at the bottom of the second tube body can be quickly absorbed by a suction tube through the central small hole, so that the cells can be thoroughly separated from the tissue block and are not easy to lose; the bottom of the second pipe body is designed to be a conical structure, so that cells passing through the cell screen can be rapidly precipitated at the bottom of the second pipe body, and the purpose of rapid separation is achieved; therefore, the tissue block can be separated from the cells through one-step centrifugation, the operation is simple, the cells are completely separated from the tissue block, and the cells are not easy to lose.

Description

Centrifuging tube device of quickly separating cells

Technical Field

The utility model belongs to the technical field of cell separation, and particularly relates to a centrifugal tube device for quickly separating cells.

Background

In recent years, in the cell culture of human or animal tissues in medical experiments, the human or animal tissues need to be crushed, digested by enzyme and the like to obtain cells, and a separation step is carried out in the process, so that a centrifuge tube device is often used; however, the tissue mass after enzyme digestion is flocculent, which is difficult to discard.

At present, a Chinese patent with publication number CN211706833U and publication date of 2020, 10 and 20 discloses a high-efficiency centrifugal tube with a novel separation kit, which comprises a centrifugal tube, wherein a partition plate and a movable kit positioned above the partition plate are arranged in the centrifugal tube, the movable kit comprises a hanging bracket and a filter screen, the hanging bracket is of a barrel-shaped structure and is embedded in the centrifugal tube, and the bottom of the hanging bracket is provided with a through hole for installing the filter screen; the side face of the hanging bracket is provided with a groove which is matched with the side wall of the centrifugal tube to form an injection channel. The device is cleaned by centrifugation and the tissue mass is removed thoroughly through a cell screen. However, in the operation process, multiple times of centrifugal cleaning are needed, and rapid separation cannot be performed at one time, so that excessive cell loss is caused; and the centrifugal tube does not adopt the design of conical structure, leads to cell sedimentation time overlength.

SUMMERY OF THE UTILITY MODEL

The utility model aims to solve the problems in the prior art, and provides a centrifugal tube device for rapidly separating cells, which can completely separate tissue blocks from the cells through one-step centrifugation, is not easy to lose the cells, and is simple to operate, rapid and efficient.

In order to achieve the purpose, the utility model adopts the following technical scheme:

a centrifuge tube device for rapidly separating cells comprises a cap, a first tube body, a second tube body and a suction tube; wherein the cap is in threaded connection with the first tube; the bottom of the first pipe body is fixedly connected with a second pipe body; the bottom of the second pipe body is of a conical structure; the straw penetrates through the cap, the first pipe body and the bottom of the second pipe body to be communicated.

Specifically, the cap is provided with a straw hole; the inner wall of the straw hole is glued and tightly attached with the rubber layer, and the straw penetrates through the rubber layer, so that the splicing is more convenient and the sealing performance is better.

Specifically, the bottom of the first pipe body is clamped with a cell screen; the cell screen is provided with a small central hole; the suction pipe penetrates through the central small hole and is connected with the bottom of the second pipe body.

Specifically, the cap is provided with an internal thread, and the pipe orifice of the first pipe body is provided with an external thread matched with the internal thread; the cap and the first pipe body are connected with the external thread in a screwing mode through the internal thread.

Specifically, the bottom of the second pipe body is of a conical structure.

Specifically, capacity scales are evenly marked on the first pipe body and the second pipe body.

Compared with the prior art, the technical scheme of the utility model has the following beneficial effects:

(1) the straw hole on the cover cap is provided with the rubber ring, so that the tightness of the centrifugal tube is better.

(2) The cell screen is provided with a central small hole, and the cell precipitated at the bottom of the second tube body can be quickly absorbed by a suction tube through the central small hole, so that the cell and the tissue block can be thoroughly separated, and the cell is not easy to lose.

(3) The bottom of the second pipe body is designed to be a conical structure, so that cells passing through the cell screen can be rapidly precipitated at the bottom of the second pipe body, and the purpose of rapid separation is achieved.

In order to make the aforementioned objects, features and advantages of the embodiments of the present application more comprehensible, embodiments accompanied with figures are described in detail below.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained from the drawings without inventive effort.

FIG. 1 is a schematic diagram of the overall structure of the centrifugal tube apparatus for rapid cell separation according to the present invention;

FIG. 2 is a schematic view of a partial structure of the centrifugal tube apparatus for rapid cell separation according to the present invention.

In the figure:

1-cap, 2-straw hole, 3-rubber layer, 4-straw, 5-internal thread, 6-external thread, 7-first tube, 8-cell screen, 9-central hole and 10-second tube.

Detailed Description

The embodiments of the present disclosure are described in detail below with reference to the accompanying drawings.

The embodiments of the present disclosure are described below with specific examples, and other advantages and effects of the present disclosure will be readily apparent to those skilled in the art from the disclosure in the specification. It is to be understood that the described embodiments are merely illustrative of some, and not restrictive, of the embodiments of the disclosure. The disclosure may be embodied or carried out in various other specific embodiments, and various modifications and changes may be made in the details within the description without departing from the spirit of the disclosure. It is to be noted that the features in the following embodiments and examples may be combined with each other without conflict. All other embodiments, which can be derived by a person skilled in the art from the embodiments disclosed herein without making any creative effort, shall fall within the protection scope of the present disclosure.

As shown in fig. 1, the present embodiment provides a centrifuge tube device for rapidly separating cells, which includes a cap 1, a first tube 7, a second tube 10, and a straw 4; the cap 1 is in threaded connection with the first tube body 7, so that the sealing performance of the centrifugal tube is better when the centrifugal tube is used for a separation experiment; the bottom of the first pipe body 7 is fixedly connected with a second pipe body 10; the bottom of the second tube 10 is a cone-shaped structure, so that cells passing through the cell screen 8 can be rapidly deposited at the bottom of the second tube 10; the suction pipe 4 penetrates through the cover cap 1, the first pipe body 7 and the bottom of the second pipe body 10 to be communicated, so that cells can be sucked conveniently and quickly, and the purpose of separation is achieved.

Further, the cap 1 is provided with a straw hole 2; the inner wall of the straw hole 2 is glued and tightly adhered with the rubber layer 3, and the straw 4 penetrates through the rubber layer 3, so that the splicing is more convenient and the sealing performance is better.

Further, the bottom of the first tube 7 is clamped with a cell screen 8; the cell screen 8 is provided with a central small hole 9; the suction pipe 4 penetrates through the central small hole 9 to be connected with the bottom of the second pipe body 10, so that cells precipitated at the bottom of the second pipe body 10 can be quickly sucked, and the purpose of quick separation is achieved.

Further, the cap 1 is provided with an internal thread 5, and the pipe orifice of the first pipe body 7 is provided with an external thread 6 matched with the internal thread 5; the cap 1 and the first tube body 7 are screwed with the external thread 6 through the internal thread 5, so that the sealing performance of the centrifugal tube is better.

Further, the bottom of the second tube 10 is a conical structure, so that cells passing through the cell screen 8 can be rapidly deposited at the bottom of the second tube 10.

Further, the first pipe body 7 and the second pipe body 10 are evenly provided with capacity scales, so that the separation experiment condition can be observed at any time, and the cell separation experiment result is more accurate.

Claims

1. The utility model provides a centrifuging tube device of quickly separating cell, includes block (1), first body (7), second body (10), straw (4), its characterized in that: the cap (1) is in threaded connection with the first pipe body (7); the bottom of the first pipe body (7) is fixedly connected with a second pipe body (10); the bottom of the second pipe body (10) is of a concave structure; straw (4) pass cap (1) first body (7) with second body (10) bottom intercommunication can absorb the cell that deposits in the centrifuging tube bottom fast, quick separation.

2. The centrifuge tube apparatus for rapidly separating cells according to claim 1, wherein: the cap (1) is provided with a straw hole (2); the inner wall of the straw hole (2) is glued and tightly attached with the rubber layer (3), and the straw (4) penetrates through the rubber layer (3), so that the sealing performance is better.

3. The centrifuge tube apparatus for rapidly separating cells according to claim 1, wherein: the bottom of the first pipe body (7) is clamped with a cell screen (8); the cell screen (8) is provided with a central small hole (9); the suction pipe (4) penetrates through the central small hole (9) and is connected with the bottom of the second pipe body (10).

4. The centrifuge tube apparatus for rapidly separating cells according to claim 1, wherein: the cap (1) is provided with an internal thread (5), and the pipe orifice of the first pipe body (7) is provided with an external thread (6) matched with the internal thread (5); the cap (1) and the first pipe body (7) are connected with the external thread (6) in a screwing mode through the internal thread (5).

5. The centrifuge tube apparatus for rapidly separating cells according to claim 1, wherein: the bottom of the second pipe body (10) is of a conical structure.

6. The centrifuge tube apparatus for rapidly separating cells according to claim 1, wherein: the first pipe body (7) and the second pipe body (10) are evenly marked with capacity scales.