CN210545211U

CN210545211U - Platelet-rich plasma separation device

Info

Publication number: CN210545211U
Application number: CN201921060268.9U
Authority: CN
Inventors: 张小波; 谢晓燕; 杨劲鹏
Original assignee: Shaanxi Yisaier Biotechnology Co Ltd
Current assignee: Shaanxi Yisaier Biotechnology Co Ltd
Priority date: 2019-07-09
Filing date: 2019-07-09
Publication date: 2020-05-19
Anticipated expiration: 2029-07-09

Abstract

The utility model belongs to the biotechnology field, in particular to a platelet-rich plasma separation device; the device comprises a cylindrical pipe body with uniform inner diameter, wherein a hose with a needle is arranged at the lower end of the pipe body and is communicated with the pipe body, a sealing plug is arranged on the inner wall of the pipe body, and a push rod is arranged at the upper end of the sealing plug; the upper end and the lower end of the tube body are respectively provided with an end cover connecting position which is movably connected with an end cover, and the end cover is matched with the centrifuge; on one hand, the utility model is provided with a plurality of hoses with needles, which are divided into tubes for sampling blood, separating PRP and other liquids, thus improving the purity of the PRP; on the other hand, the end cover is movably connected with the upper end or the lower end of the tube body, and the end cover is matched with the centrifugal machine for centrifugation, so that the collection and centrifugation work is carried out in the same tube body, and the pollution rate of PRP separation is greatly reduced.

Description

Platelet-rich plasma separation device

Technical Field

The utility model belongs to the technical field of medical instrument, concretely relates to rich platelet plasma separator.

Background

Platelet-rich plasma (PRP) is a platelet concentrate obtained by centrifuging peripheral blood for many times, can release various active substances such as cytokines, chemokines, growth factors and the like, and can promote wound healing and tissue repair through different regeneration mechanisms under different pathological microenvironments. Many researchers find that active substances such as growth factors released by PRP have the effects of promoting cell proliferation, collagen synthesis and inflammatory chemotaxis, and PRP has the advantages of simple and convenient preparation, convenient use, low cost and the like. The collagen peptide is mainly applied to medical cosmetology, lumbago and other pain diseases, alopecia, skin burn, skin regeneration, orthopedic cartilage, tendon, ligament and the like in medical profession for nearly thirty years, and the mechanism mainly comprises the functions of repairing, resisting inflammation and promoting wound healing by releasing various growth factors, promoting the synthesis of proteoglycan and collagen and stimulating the generation of stem cells and endogenous Hyaluronic Acid (HA). In addition, PRP can also relieve edema, scar and pain and reduce infection rate, which shows more remarkable application value in the field of cosmetic plastic. The versatility and good biocompatibility and its simple preparation method promote its application in many medical fields, including orthopedics, periodontal, oral surgery, maxillofacial surgery, cardiac surgery, plastic surgery, sports medicine and other disciplines.

High quality PRP should be free of red blood cells, have low plasma levels, and have platelet concentrations 3-5 times that of whole blood. High quality PRP is more conducive to extraction of the desired active substance for optimal medical use.

However, most of separation tubes used for separating PRP in the prior art are straight cylinders, blood samples, PRP, plasma and the like can enter and exit from the same opening, the required PRP purity requirement cannot be met, and the separation operation process can be repeatedly exposed to the outside, so that certain pollution is caused.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a rich platelet plasma separator to the PRP purity of the current separator separation that mentions among the solution prior art is low, and can cause the problem of certain pollution.

The technical scheme adopted by the utility model is a platelet-rich plasma separation device, which comprises a cylindrical pipe body with uniform inner diameter, wherein the lower end of the pipe body is provided with a hose with a needle, the hose with the needle is communicated with the pipe body, the inner wall of the pipe body is provided with a sealing plug, and the upper end of the sealing plug is provided with a push rod; the upper end and the lower end of the tube body are respectively provided with an end cover connecting position, the end cover connecting position is movably connected with an end cover, and the end cover is matched and connected with the centrifugal machine.

By arranging a plurality of hoses with needles, the tube is divided for blood sampling, PRP separation and other cell separation, and the purity of the PRP separation is improved; on the other hand, the end cover is movably connected with the upper end or the lower end of the tube body, and meanwhile, the end cover is matched with the centrifugal machine to perform centrifugal work, so that the collection and the centrifugal work are performed in the same tube body, and the pollution rate of PRP separation is greatly reduced. .

Preferably, the sealing plug is detachably and movably connected with the push rod.

The detachable movable connection can be in threaded connection or clamping connection; need the installation push rod when taking a blood sample and collecting the PRP, when the separation PRP, need dismantle the push rod to reduce the device volume, make the blood sampling, centrifugation and collection all go on in same body, reduce the pollution rate.

Preferably, the sealing plug is provided with a sample adding groove.

The sample adding groove can be arranged to be thin, so that an injector is easy to use to add anticoagulant or other liquid into the tube body.

Preferably, the end cover is provided with hose outlet notches, and the number and the positions of the hose outlet notches are consistent with those of hoses with needles.

The end cover is connected with a centrifuge for centrifugal work, in order not to influence the installation and the use of the end cover, if the end cover is installed at the lower end of the tube body, the hose notches can be arranged, and the number and the positions of the hose notches are consistent with those of hoses with needles.

Preferably, a plurality of supporting parting strips are arranged on the inner side of the end cover.

The end cover can be supported by the plurality of supporting parting beads, and the end cover is directly connected with the centrifuge for centrifugal work, so that the plurality of supporting parting beads also increase the service pressure of the end cover.

Preferably, the hose fixing structure is that a clamping groove ring is arranged on the outer side wall of the hose body, and a plurality of hose clamping grooves are formed in the clamping groove ring.

Because use same body to take a blood sample and centrifugal work, for the convenience of going on in order of centrifugal work, set up hose fixed knot structure on the body lateral wall and fix the hose of taking the needle, but hose draw-in groove joint hose of taking the needle or needle cap.

Preferably, the lower end of the inner wall of the pipe body is a conical body.

When PRP is collected, the PPP layer on the upper side of the PRP layer or the erythrocyte layer on the lower side of the PRP layer is firstly drained, so that the PPP layer or the erythrocyte layer is drained completely, or the lower end of the inner wall of the tube body is set to be a conical body.

Preferably, a needle cap is arranged on the needle head of the hose with the needle, and a groove is formed in the joint of the needle cap and the hose with the needle.

The needle cap is arranged on the needle head to improve the sealing performance and reduce the pollution rate; the groove is arranged at the joint of the needle cap and the hose with the needle, so that the hose is easy to break under the condition that the needle is not used any more.

Preferably, one side of the hose with the needle, which is close to the hose body, is provided with a liquid stopping clip.

The use of the liquid stopping clamp enables a closed space to be formed in the tube body, and the centrifugation and separation work can be conveniently and orderly carried out.

Preferably, the needle cap is sealingly connected to the hose with the needle.

The needle cap is connected with the hose with the needle in a sealing way, so that the sealing performance is improved, and the pollution rate is reduced to the maximum extent.

The utility model has the advantages that:

on one hand, the utility model is provided with a plurality of hoses with needles, which are divided into tubes for sampling blood, separating PRP and other liquids, thus improving the purity of the PRP; on the other hand, the end cover is movably connected at the upper end or the lower end of the tube body, and meanwhile, the end cover is matched with the centrifugal machine for centrifugation, so that the collection and centrifugation work is carried out in the same tube body, and the pollution rate of PRP separation is greatly reduced.

Drawings

Fig. 1 is a schematic structural view of a platelet-rich plasma separation device of the present invention;

FIG. 2 is a schematic structural view of the tube body of the present invention;

FIG. 3 is a schematic structural view of the push rod of the present invention;

fig. 4 is a schematic structural view of the hose fixing structure of the present invention;

fig. 5 is a schematic perspective view of the end cap of the present invention;

fig. 6 is a schematic top view of the end cap of the present invention.

Description of reference numerals: 1-a pipe body; 2-hose with needle; 201-needle cap; 202-grooves; 203-liquid stopping clip; 3-sealing the plug; 301-sample addition slot; 4-a push rod; 5-a hose fixing structure; 501-a clamping groove ring; 502-card slot; 6-end cover connection position; 7-end cap; 701-hose notch; 702-supporting the division bar.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings and detailed description, and it is to be understood that the described embodiments are only some embodiments, not all embodiments, of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative efforts belong to the protection scope of the present invention.

In the description of the present invention, it is to be understood that the terms "central, lateral, longitudinal, up, down, front, back, left, right, vertical, horizontal, top, bottom, inner, outer" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in fig. 1 only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

The utility model relates to a platelet-rich plasma separation device, the structural schematic diagram of which is shown in figure 1, comprising a cylindrical tube body 1 with uniform inner diameter, a hose with needles 2 arranged at the lower end of the tube body 1, the hose with needles 2 communicated with the tube body 1, a sealing plug 3 arranged on the inner wall of the tube body 1, a push rod 4 arranged at the upper end of the sealing plug 3, at least two hoses with needles 2, and a hose fixing structure 5 arranged on the outer side wall of the tube body 1; the upper end and the lower end of the tube body 1 are respectively provided with an end cover connecting position 6, the end cover connecting position 6 is movably connected with an end cover 7, and the end cover 7 is matched and connected with the centrifuge.

By arranging a plurality of hoses 2 with needles, the tube is divided for blood sampling, PRP separation and other cell separation, so that the purity of PRP separation is improved; through at body 1 upper end or lower extreme swing joint end cover 7, end cover 7 carries out centrifugal work with the centrifuge cooperation simultaneously, makes collection and centrifugal work go on in same body 1, uses one-step centrifugation method or two-step centrifugation method all can, greatly reduced the pollution rate of separation PRP.

The structure of the tube is schematically shown in fig. 2, except for the sealing plug 3 and the push rod 4, other parts of the device are made of transparent materials meeting medical conditions. When the PRP is collected, the PPP layer on the upper side of the PRP layer or the erythrocyte layer on the lower side thereof is removed, and the lower end of the inner wall of the tube body 1 may be tapered to remove the PPP layer or the erythrocyte layer. The outer side wall of the tube body 1 is provided with scales, so that the volume of anticoagulant and the blood sampling volume can be conveniently measured.

The schematic diagram of the push rod is shown in fig. 3, the sealing plug 3 and the push rod 4 are detachably and movably connected, the detachable and movable connection enables the push rod 4 to be installed and detached at any time according to needs, the device is convenient to package and transport, the requirement on the centrifugal radius is reduced, meanwhile, the possibility is provided for properly expanding the volume of the device, and the application range of the device is wider. The sealing plug 3 can slide on the inner wall of the tube body 1 through the pushing force and the pulling force of the push rod 4, and the sealing performance is excellent. One of the movable connection modes is as follows: the upper end of the sealing plug 3 is provided with an internal thread, the lower end of the push rod 4 is provided with an external thread, and the external thread is connected with the external thread through a thread to perform detachable action; the other movable connection mode is as follows: an inner buckle is arranged at the upper end of the sealing plug 3, an outer buckle is arranged at the lower end of the push rod 4, and the inner buckle is connected through the buckle to perform detachable action.

When blood sampling is needed, a push rod 4 is installed for blood sampling; when centrifugation is carried out, the push rod 4 is detached, the end cover 7 is installed, and then the centrifugal machine is connected for centrifugation; when PRP is separated after centrifugation is finished, the push rod 4 is mounted again for separation. The sealing plug 3 and the push rod 4 are detachably and movably connected, so that the size of the device can be greatly reduced, and the end cover 7 is convenient to mount for centrifugation, so that blood sampling and centrifugation are carried out in the same tube body 1, and the pollution rate of PRP separation is greatly reduced.

Pour into the anticoagulant in advance in body 1 before taking a blood sample, then be equipped with application of sample groove 301 on sealing plug 3, the material of sealing plug 3 is preferably rubber, and rubber has the characteristic of good reversible deformation, and the leakproofness is good, and application of sample groove 301 on sealing plug 3 can set up thinner, easily uses the syringe to add the anticoagulant, and the anticoagulant selects ACD-A anticoagulant. Meanwhile, the collected blood sample can be injected into the tube body 1 through the sample adding groove 301, so that the separation of the indirectly collected blood sample is realized.

The needle cap 201 is arranged on the needle head of the needle-containing hose 2, the needle cap 201 is hermetically connected with the needle-containing hose 2, and the arrangement of the needle cap 201 and the sealing of the joint of the needle cap enable the platelet-rich plasma separation device to reduce the pollution rate; the groove 202 is arranged at the joint of the needle cap 201 and the hose 2 with the needle, the hose is easy to break due to the groove 202, namely, after the blood is collected, the needle head for collecting blood is not used any more, and can be broken, so that the centrifuge is more convenient to centrifuge; the liquid stopping clamp 203 is arranged on one side, close to the tube body 1, of the hose with the needle 2, and due to the use of the liquid stopping clamp 203, a closed space is formed in the tube body 1, so that the centrifugation and separation work can be conveniently carried out.

In order to facilitate the orderly centrifugal work, the hose with needles 2 needs to be fixed on the tube body 1, and the hose fixing structure 5 can be arranged on the outer side wall of the tube body 1 to fix the hose with needles 2. The hose fixing structure 5 may be formed by providing a plurality of hose engaging grooves only on the outer side wall of the pipe body 1. Another structure diagram of the hose fixing structure is shown in fig. 4, the hose fixing structure 5 may also be formed by arranging a groove ring 501 around the outer side wall of the tube body 1, the groove ring 501 is provided with a plurality of hose clamping grooves 502, the groove ring 501 is fixedly connected with the outer wall of the tube body 1, and the hose clamping grooves 502 may be used for clamping the hose with needles 2 or the needle cap 201.

The end cover 7 is movably connected with the end cover connecting position 6, and the end cover 7 can be in threaded connection or clamped connection when being connected with the end cover connecting position 6 at the upper end of the tube body 1; when the end cover 7 is connected with the end cover connecting position 6 at the lower end of the tube body 1, the connection can be clamped or sleeved. As shown in fig. 5 and 6, when the end cap 7 is mounted at the lower end of the tube body 1, since the tube body 1 is provided with a plurality of hoses 2 with needles at the lower end, a corresponding number of hose notches 701 are provided at corresponding positions on the end cap 7 according to the number and positions of the hoses 2 with needles. Since the end cap 7 is connected to a centrifuge for centrifugation, a plurality of support spacers 702 are provided inside the end cap 7 in order to support the shape of the end cap 7 and increase the pressure for using the end cap 7. The shape of the end cap 7 can be selected according to the installation position, for example, if the end cap is installed on the upper end of the tube body 1, the conventional end cap is selected, i.e., the end cap is provided with the far hose notch 701, but a plurality of supporting spacers 702 are provided, and of course, the end cap 7 with the hose notch 701 can be selected when the end cap 7 is installed on the upper end of the tube body 1.

When the platelet-rich plasma separation device of the utility model is used for separating PRP, the separation method specifically comprises the following steps:

step 1, preparing before blood sample collection, closing all the liquid stopping clamps 203, and adding a certain volume of anticoagulant into the tube body 1 in advance.

And 2, collecting a blood sample, installing a push rod 4, collecting the blood sample with a certain volume by using the hose with a needle 2, and reversing the tube body for multiple times after the collection is finished to prepare for next centrifugation.

And 2.1, installing a push rod 4 to movably connect the push rod 4 with the sealing plug 3.

And 2.2, disinfecting the vein collection position of the collector, puncturing the vein of the collector by using any hose 2 with a needle, opening the liquid stopping clamp 203, slowly pulling the push rod 4 to collect the blood volume with the required volume, closing the liquid stopping clamp 203, pulling out the needle head, sealing by using the needle cap 201, and fixing the hose 2 with the needle in the hose fixing structure 5.

And 2.3, disassembling the push rod 4, and reversing the tube body 1 for multiple times to prepare for next centrifugation.

Step 3, separating and collecting the platelet-rich plasma by using a one-step centrifugal separation method or a two-step centrifugal separation method, wherein after centrifugation, the upper layer is a PPP layer (namely a yellow transparent platelet-poor plasma layer), the middle layer is a PRP layer (namely a pale yellow slightly turbid platelet-rich plasma layer), and the lower layer is a red erythrocyte layer;

when a one-step centrifugal separation method is adopted, the end cover connecting position 6 at the upper end of the tube body 1 obtained in the step 2 is movably connected with the end cover 7, one end provided with the end cover 7 is inserted into a centrifuge for centrifugation, after the centrifugation is finished, the device is vertically taken out, the end cover 7 is removed at the moment, the push rod 4 is installed, the push rod 4 is kept perpendicular to the table top, the push rod 4 is slowly extruded, the PPP layer is discharged through the other tube 2 with the needle until turbid light yellow PRP can be seen at the tube, and then the PRP is collected into a sterile collection container by using the other tube 2 with the needle until red erythrocyte liquid can be seen at the tube;

when a two-step centrifugal separation method is adopted, the end cover connecting position 6 at the lower end of the tube body 1 obtained in the step 2 is movably connected with the end cover 7, one end provided with the end cover 7 is inserted into a centrifuge for centrifugation, after the centrifugation is finished, the device is vertically taken out, the end of the hose with the needle 2 faces downwards at the moment, the end cover 7 is removed, the push rod 4 is installed, the push rod 4 is kept vertical to the table top, the push rod 4 is slowly extruded, and a red erythrocyte layer is discharged through the other hose with the needle 2 until a turbid light yellow PRP layer can be seen at the hose; and then the push rod 4 is disassembled, the end cover connecting position 6 at the lower end of the tube body 1 is movably connected with the end cover 7, one end provided with the end cover 7 is inserted into the centrifuge for re-centrifugation, after the re-centrifugation is finished, the device is vertically taken out, the end cover 7 is removed, the push rod 4 is installed, the residual erythrocyte layer is discharged through another hose 2 with a needle until a turbid light yellow PRP layer is visible at the hose, the PRP platelet-rich plasma is collected to a sterile collection container by using the other hose 2 with the needle, and one third of the total volume of the second centrifugation is collected to be the required platelet-rich plasma.

And 4, storing the PRP collection container for later use.

In the step 1, according to the volume of the blood to be collected: ACD-a anticoagulant volume 40: 7. when anticoagulant is added, the anticoagulant is added from the sample addition tank 301 using a syringe. Other satisfactory anticoagulants may also be used.

In the one-step centrifugation in step 3, the centrifugation is carried out for about 15 minutes every 1400 g. Centrifuging for about 10 minutes per 400g in the first centrifugation of the two-step centrifugation method; in the second centrifugation of the two-step centrifugation method, centrifugation was carried out for about 10 minutes per 800 g.

In summary, in order to solve the problems of the prior art, the purity of PRP separated by the prior separation device is low, and certain pollution is caused. On one hand, the utility model is provided with a plurality of hoses with needles, which are divided into tubes for blood sampling, PRP separation and other cell separation, thereby improving the purity of PRP separation; on the other hand, the end cover is movably connected with the upper end or the lower end of the tube body, and meanwhile, the end cover is matched with the centrifugal machine to perform centrifugal work, so that the collection and the centrifugal work are performed in the same tube body, and the pollution rate of PRP separation is greatly reduced.

In the description herein, references to the description of "one embodiment," "an example," "a specific example," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The preferred embodiments of the present invention disclosed above are intended only to help illustrate the present invention. The preferred embodiments are not exhaustive and do not limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and its practical applications, to thereby enable others skilled in the art to best understand the invention for and utilize the invention. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A platelet-rich plasma separation device comprises a cylindrical tube body (1) with a uniform inner diameter, wherein a needle-carrying hose (2) is arranged at the lower end of the tube body (1), the needle-carrying hose (2) is communicated with the tube body (1), a sealing plug (3) is arranged on the inner wall of the tube body (1), and a push rod (4) is arranged at the upper end of the sealing plug (3), and the platelet-rich plasma separation device is characterized in that at least two needle-carrying hoses (2) are arranged, and a hose fixing structure (5) is arranged on the outer side wall of the tube body (1);

the centrifugal centrifuge is characterized in that end cover connecting positions (6) are respectively arranged at the upper end and the lower end of the tube body (1), the end cover connecting positions (6) are movably connected with end covers (7), and the end covers (7) are connected with the centrifuge in a matched mode.

2. The platelet-rich plasma separation device according to claim 1, wherein the sealing plug (3) is detachably and movably connected with the push rod (4).

3. The platelet-rich plasma separation device according to claim 2, wherein the sealing plug (3) is provided with a sample addition slot (301).

4. The platelet-rich plasma separation device according to claim 1, wherein the end cap (7) is provided with outlet tube notches (701), and the number and positions of the outlet tube notches (701) are consistent with those of the flexible tubes (2) with needles.

5. The platelet-rich plasma separation device according to claim 4, wherein the inside of the end cap (7) is provided with a plurality of support spacers (702).

6. The platelet-rich plasma separation device according to claim 1, wherein the hose fixing structure (5) is a snap ring (501) disposed on the outer side wall of the tube body (1), and the snap ring (501) is provided with a plurality of hose snap grooves (502).

7. The platelet-rich plasma separation device according to claim 1, wherein the lower end of the inner wall of the tube body (1) is a cone.

8. The platelet-rich plasma separation device according to claim 1, wherein a needle cap (201) is provided on the needle of the needle-carrying flexible tube (2), and a groove (202) is provided at the joint of the needle cap (201) and the needle-carrying flexible tube (2).

9. The platelet-rich plasma separation device according to claim 8, wherein the side of the needle-carrying hose (2) close to the tube body (1) is provided with a liquid stopping clip (203).

10. The platelet-rich plasma separation device according to claim 8, wherein the needle cap (201) is sealingly connected to the needle-carrying hose (2).