CN214991543U

CN214991543U - Single cell cracking and separating device

Info

Publication number: CN214991543U
Application number: CN202120534627.0U
Authority: CN
Inventors: 赵海波; 于腾波; 李富江; 尚宝欣; 宋文联; 张浩运; 郭珈
Original assignee: Affiliated Hospital of University of Qingdao
Current assignee: Affiliated Hospital of University of Qingdao
Priority date: 2021-03-15
Filing date: 2021-03-15
Publication date: 2021-12-03
Anticipated expiration: 2031-03-15

Abstract

The utility model relates to a cell experiment auxiliary assembly technical field specifically discloses a unicellular schizolysis separator. The saliva collecting tube comprises a U-shaped saliva collecting tube, wherein one end of the saliva collecting tube is detachably communicated with a suction tube, the other end of the saliva collecting tube is detachably communicated with an air guide tube, and the lowest end of the saliva collecting tube is detachably communicated with a saliva collecting bottle; the free end of air duct can be dismantled and be connected with the unicellular collection chamber, and the unicellular collection chamber bottom surface runs through and is equipped with the function pipe, and the terminal surface that the function pipe is located unicellular collection intracavity one end is higher than the interior top surface in unicellular collection chamber, and the function pipe is located the outer one end of unicellular collection chamber and can be dismantled and be connected with glass needle tubing. The utility model aims to solve traditional oral siphon when carrying out the unicellular separation, operating personnel can flow its saliva into the unicellular colony culture dish, thereby cause the problem that the unicellular colony culture dish is polluted and influences the experiment progress.

Description

Single cell cracking and separating device

Technical Field

The application relates to the technical field of cell experiment auxiliary equipment, and particularly discloses a single cell cracking and separating device.

Background

In cell-related experiments, it is often necessary to sort cells from a population into single cells in order to study the function of single cells, or to sequence single cells, or to genetically edit a population of cells to obtain single cells with a particular editing state.

The current methods of sorting cells from a population into individual cells are mainly: the present invention relates to a single cell lysis separation device, and more particularly, to a single cell lysis separation device, which comprises a mouth pipette technique, a micromanipulation technique, a laser microdissection method, and a flow cytometry method, wherein the mouth pipette technique is used for single cell separation, and can select cells with good morphology under a microscope, and the cells are almost not damaged, so that the success rate of downstream experiments is very high, and compared with other techniques, the cost of the mouth pipette technique is low.

Disclosure of Invention

The utility model aims to solve traditional oral siphon when carrying out the unicellular separation, operating personnel can flow its saliva into the unicellular colony culture dish, thereby cause the problem that the unicellular colony culture dish is polluted and influences the experiment progress.

In order to achieve the aim, the basic scheme of the utility model provides a unicellular cracking separation device, which comprises a U-shaped saliva collecting pipe, wherein one end of the saliva collecting pipe is detachably communicated with a suction pipe, the other end of the saliva collecting pipe is detachably communicated with an air duct, and the lowest end of the saliva collecting pipe is detachably communicated with a saliva collecting bottle;

the free end of air duct can be dismantled and be connected with the unicellular collection chamber, and the unicellular collection chamber bottom surface runs through and is equipped with the function pipe, and the terminal surface that the function pipe is located unicellular collection intracavity one end is higher than the interior top surface in unicellular collection chamber, and the function pipe is located the outer one end of unicellular collection chamber and can be dismantled and be connected with glass needle tubing.

The principle and effect of this basic scheme lie in:

1. connect together straw, saliva collecting pipe, air duct, unicellular collection chamber, functional tube and glass needle tubing in proper order, form one and be similar to present complete mouth suction tube, then carry out the schizolysis work through lysate etc. with the unicellular crowd and make every unicellular all be in the free state, then the operator mouth contains the straw, utilize the microscope and insert the unicellular culture dish alright begin to carry out the work of separating in proper order of unicellular.

2. When using the straw for a long time, because reasons such as the operator is not skilled, the saliva of operator probably flows to glass needle tubing one end through the straw, and in this device, has added a U-shaped saliva collecting pipe between straw and glass needle tubing, then the saliva that flows into the straw will be followed trend and flowed into the saliva collecting pipe, and the lowermost extreme of saliva collecting pipe can be dismantled and be connected with the saliva collecting bottle, and the saliva that flows into the saliva collecting pipe at last just can be collected in the saliva collecting bottle, has effectively prevented subsequent lysate of unicellular separation usefulness of saliva pollution etc..

3. When the single cell is separated, the end face of one end of the functional tube, which is positioned in the single cell collecting cavity, is higher than the inner top face of the single cell collecting cavity, so that the functional tube can be sucked into the single cell collecting cavity through the glass needle tube, and the separated single cell can be temporarily stored according to the use separation requirement.

Compared with the prior art, the oral pipette device of the utility model can conveniently and effectively sort the group cells into single cells, has low cost and can be used repeatedly, and the activity of the single cells manually sorted by the oral pipette is high; meanwhile, saliva of an operator can be effectively prevented from polluting the unicellular culture solution or lysate, the survival rate of primary cells after sorting is greatly improved, and the method is simple to operate, high in feasibility and convenient to popularize and use.

Furthermore, the free end of the suction pipe is detachably connected with a suction nozzle. The suction nozzle can be replaced when in use, thereby being convenient for different users to use.

Furthermore, the lowest end of the saliva collecting tube is communicated with a connecting tube, and the saliva collecting bottle is detachably connected with the saliva collecting tube through the connecting tube. The saliva collecting tube is convenient to be connected with the saliva collecting bottle.

Furthermore, a plurality of partition grooves are formed in the bottom surface of the single cell collection cavity, a plurality of discharging pipes corresponding to the partition grooves are communicated with the bottom surface of the single cell collection cavity, the free ends of the discharging pipes can be detachably connected with the single cell collection bag, the functional pipe is rotatably connected with the bottom surface of the single cell collection cavity, and the functional pipe is communicated with one end of the single cell collection cavity and is provided with an expansion pipe corresponding to the partition grooves. When the single-cell separation device is used, the function rod can be rotated, so that the expansion pipe on the function rod rotates to the upper side of the corresponding separation groove, then a plurality of separated single cells can be sequentially collected when the single-cell separation device is used, the single cells sequentially enter the separation groove and flow into the single cell collection bag along the trend, the single cell separation work is finished, and the subsequent movement is convenient.

Furthermore, a supporting pipe is fixedly connected to the bottom surface of the single cell collecting cavity in a penetrating manner, and the functional pipe is rotatably connected in the supporting pipe. The rotational stability of the functional tube is improved.

Furthermore, a rubber hose is arranged between the functional tube and the glass needle tube. The operating flexibility of the glass needle tube is improved.

Further, the single cell collection pouch is a squeezable rubber pouch. So as to facilitate the extrusion of the single cell entering the single cell collecting capsule.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.

FIG. 1 is an exploded view of a single-cell lysis separation apparatus according to an embodiment of the present application;

FIG. 2 is a schematic diagram showing a single-cell collection chamber in a single-cell lysis separation device according to an embodiment of the present application.

Detailed Description

To further illustrate the technical means and effects of the present invention adopted to achieve the predetermined objects, the following detailed description of the embodiments, structures, features and effects according to the present invention will be made with reference to the accompanying drawings and preferred embodiments.

Reference numerals in the drawings of the specification include: the device comprises a suction nozzle 1, a suction tube 2, a saliva collecting tube 3, a connecting tube 4, a saliva collecting bottle 5, an air duct 6, a single cell collecting cavity 7, a discharging tube 8, a single cell collecting bag 9, a functional tube 10, a rubber hose 11, a glass needle tube 12, an expansion tube 13 and a dividing groove 14.

For example, as shown in fig. 1, the left end of a suction tube 2 is connected with a suction nozzle 1 in a sliding and sealing manner, then the lower end of the suction tube 2 is connected with the left end of a U-shaped saliva collecting tube 3 in a sliding and sealing manner, the other end of the saliva collecting tube 3 is connected with an air duct 6 in a sliding and sealing manner, the lowest end of the saliva collecting tube 3 is communicated with a connecting tube 4, and a saliva collecting bottle 5 is connected with the saliva collecting tube 3 in a sliding and sealing manner through the connecting tube 4.

As shown in fig. 1, the lowest end of the air duct 6 is connected with a single cell collecting cavity 7 in a sliding and sealing way, as shown in figure 2, a supporting tube is fixedly connected to the bottom surface of the single cell collecting cavity 7 in a penetrating manner, a functional tube 10 is rotatably connected in the supporting tube, a plurality of dividing grooves 14 are arranged on the inner bottom surface of the single cell collecting cavity 7, the end surface of one end of the functional tube 10 in the single cell collecting cavity 7 is higher than the dividing grooves of the single cell collecting cavity 7, an extension tube 13 corresponding to the dividing grooves 14 is communicated with one end of the functional tube 10 in the single cell collecting cavity 7, a plurality of discharging tubes 8 corresponding to the dividing grooves 14 are communicated with the inner bottom surface of the single cell collecting cavity 7, as shown in FIG. 1, a single cell collecting bag 9 is connected to the lowest end of the discharging tube 8 in a sliding and sealing manner, the single cell collecting bag 9 is a squeezable rubber bag, and one end of the functional tube 10, which is located outside the single cell collecting cavity 7, is connected with a glass needle tube 12 through a rubber hose 11.

The utility model provides a sliding seal connected mode does, and the outer warp that inserts the end slightly is greater than the internal diameter that is inserted the end, then is inserted the end and all can take place elastic deformation, consequently can not have liquid or gaseous from the link leakage after inserting, also can insert the end through external force simultaneously and extract, work such as convenient washing.

When the device is used, the suction tube 2, the saliva collecting tube 3, the air duct 6, the single cell collecting cavity 7, the functional tube 10 and the glass needle tube 12 are sequentially connected together to form a complete oral suction tube 2 similar to the prior art, then single cell groups are subjected to cracking work through lysate and the like to enable each single cell to be in a free state, then an operator holds the suction tube 2 in the mouth, and the sequential separation work of the single cells can be started by using a microscope and inserting a single cell culture dish; when the straw 2 is used for a long time, saliva of an operator may flow to one end of the glass needle tube 12 through the straw 2 due to reasons such as unskilled operators and the like, in the device, the U-shaped saliva collecting tube 3 is added between the straw 2 and the glass needle tube 12, then the saliva flowing into the straw 2 flows into the saliva collecting tube 3 along with the current situation, the lowest end of the saliva collecting tube 3 is connected with a saliva collecting bottle 5, the saliva flowing into the saliva collecting tube 3 can be collected in the saliva collecting bottle 5, and the saliva is effectively prevented from polluting lysate and the like for subsequent single cell separation.

When the single-cell separation device is used, the function rod can be rotated, so that the expansion pipe on the function rod rotates to the position above the corresponding separation groove 14, then a plurality of separated single cells can be sequentially collected when the single-cell separation device is used, the single cells sequentially enter the separation groove 14 and flow into the single cell collection bag 9 in a homeopathic manner, the single cell separation work is completed, and the subsequent movement is facilitated.

After use, various components can be cleaned, the single cell collecting bag 9 containing single cells can be recovered and the like according to the use requirements.

The oral suction tube 2 device of the utility model can conveniently and effectively sort the group cells into single cells, has low cost and can be used repeatedly, and the activity of the single cells manually sorted by the oral suction tube 2 is high; meanwhile, saliva of an operator can be effectively prevented from polluting the unicellular culture solution or lysate, the survival rate of primary cells after sorting is greatly improved, and the method is simple to operate, high in feasibility and convenient to popularize and use.

Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes, substitutions and alterations can be made herein without departing from the spirit and scope of the invention as defined by the appended claims.

Claims

1. A single cell lysis separator which characterized in that: the saliva collecting tube comprises a U-shaped saliva collecting tube, wherein one end of the saliva collecting tube is detachably communicated with a suction tube, the other end of the saliva collecting tube is detachably communicated with an air guide tube, and the lowest end of the saliva collecting tube is detachably communicated with a saliva collecting bottle;

2. A single-cell lysis separation device according to claim 1, wherein a suction nozzle is detachably connected to the free end of said suction tube.

3. A single-cell lysis separation device according to claim 1, wherein the lowest end of said saliva collecting tube is connected with a connecting tube, and the saliva collecting bottle is detachably connected with the saliva collecting tube through the connecting tube.

4. A single-cell lysis and separation device as claimed in claim 1, wherein the bottom surface of the single-cell collection chamber is provided with a plurality of dividing grooves, the bottom surface of the single-cell collection chamber is communicated with a plurality of discharge pipes corresponding to the dividing grooves, the free ends of the discharge pipes are detachably connected with the single-cell collection bag, the functional pipe is rotatably connected with the bottom surface of the single-cell collection chamber, and the end of the functional pipe in the single-cell collection chamber is communicated with an extension pipe corresponding to the dividing grooves.

5. The single-cell lysis and separation device according to claim 4, wherein a support tube is fixedly connected to the bottom surface of the single-cell collection chamber, and the functional tube is rotatably connected to the support tube.

6. A single-cell lysis separation device according to claim 1, wherein a rubber hose is arranged between said functional tube and said glass needle tube.

7. A single-cell lysis separation device according to claim 4, wherein said single-cell collection pouch is a squeezable rubber pouch.