CN211847931U

CN211847931U - Menstrual blood separator

Info

Publication number: CN211847931U
Application number: CN201922498048.0U
Authority: CN
Inventors: 邱建华
Original assignee: Guangdong Yuankang Regenerative Medicine Technology Development Co ltd
Current assignee: Guangdong Yuankang Regenerative Medicine Technology Development Co ltd
Priority date: 2019-12-31
Filing date: 2019-12-31
Publication date: 2020-11-03
Anticipated expiration: 2029-12-31

Abstract

The utility model discloses a menstrual blood separator, it includes filter-pressing room, first water conservancy diversion room, first filter chamber, second water conservancy diversion room, second filter chamber, third water conservancy diversion room and the third filter chamber that from the top down set gradually. The utility model discloses a menstrual blood separator can directly carry out the selectivity to the menstrual blood collection appearance that contains absorbent material and separate, has reduced the contaminated probability of menstrual blood, and separation effect is good, can directly obtain endometrium stem cell, erythrocyte, leucocyte and plasma in the menstrual blood, has simplified follow-up work of collecting endometrium stem cell, has also improved the success rate of acquireing endometrium stem cell.

Description

Menstrual blood separator

Technical Field

The utility model relates to the technical field of medical supplies, especially, relate to a menstrual blood separator.

Background

Stem cells are a population of undifferentiated cells with self-renewal and multipotential potential, and studies have reported successful repair of endometrium and pregnancy with stem cells. Recent studies by Chan RW et al have shown that stem cells with high proliferation, self-renewal and differentiation potential are present in the endometrial basement layer and are present in epithelial, mesenchymal and endothelial cells 3 types of stem cells. The endometrial stem cells are possible to become ideal seed cells for treating infertility caused by endometrial injury. The source of the endometrial stem cells is obtained from endometrium of a patient with total hysterectomy or by human abortion at present, but the source is limited and the damage is large. Musina and other researches find that endometrium mesenchymal stem cells can be separated and cultured in vitro from endometrium exfoliated cells contained in menstrual blood, the endometrium mesenchymal stem cells have the advantages of quick replication, wide source, easily obtained materials, high content, non-invasive acquisition and the like, and sensitive medical ethical problems can be avoided because menstrual blood belongs to human waste.

The separation of menstrual blood is necessary for obtaining endometrial stem cells in menstrual blood. In the prior art, the menstrual blood is generally collected by using materials with good absorption performance, such as cotton fibers and the like, and during separation, the cotton fibers and the like are firstly pre-separated from the menstrual blood, and then the pre-separated menstrual blood is put into separation equipment to realize further separation. Because the intermediate links are more, the probability of menstrual blood pollution is greatly increased.

SUMMERY OF THE UTILITY MODEL

The utility model aims to solve the defects existing in the prior art and provide a menstrual blood separator. The separator can directly separate the menstrual blood collection sample containing the absorption material, reduces the probability of menstrual blood pollution, has good separation effect, and improves the success rate of obtaining endometrial stem cells from the menstrual blood.

In order to achieve the purpose, the utility model adopts the technical proposal that:

a menstrual blood separator comprises a filter-pressing chamber, a first diversion chamber, a first filter chamber, a second diversion chamber, a second filter chamber, a third diversion chamber and a third filter chamber which are arranged from top to bottom in sequence; two oppositely arranged press plates are arranged inside the filter pressing chamber, a pneumatic telescopic rod for driving the press plates to horizontally reciprocate is arranged on the inner side wall surface of the filter pressing chamber, and a control switch for controlling the pneumatic telescopic rod to work is arranged on the outer side wall surface of the filter pressing chamber; a first centrifugal cylinder and a first motor for driving the first centrifugal cylinder to rotate are arranged in the first filtering chamber, and a first filtering membrane is arranged on the side wall of the first centrifugal cylinder; a first guide pipe for communicating the filter-pressing chamber and the first centrifugal cylinder is arranged in the first guide chamber; a second centrifugal cylinder and a second motor for driving the second centrifugal cylinder to rotate are arranged in the second filtering chamber, and a second filtering membrane is arranged on the side wall of the second centrifugal cylinder; a second guide pipe for communicating the first filter chamber with the second centrifugal cylinder is arranged in the second guide chamber; a third filter membrane is arranged in the third filter chamber and divides the third filter chamber into an upper chamber and a lower chamber, an output pipe is arranged at the bottom of the lower chamber, a negative pressure collecting container is connected to the output pipe, and digestive juice is contained in the negative pressure collecting container; and a third flow guide pipe for communicating the second filter chamber with the upper chamber is arranged in the third flow guide chamber.

Further, a cover is arranged at the top of the filter pressing chamber. Therefore, the material absorbed with menstrual blood is conveniently placed in the filter pressing chamber.

Preferably, the pressing plate is provided with through holes which are uniformly distributed. Thus, the menstrual blood on the material can be rapidly filtered and pressed out.

Preferably, the size of the filtration pore of the first filtration membrane is 50-60 microns.

Preferably, the size of the filtration pore of the second filtration membrane is 15-25 microns.

Preferably, the filter pore size of the third filter membrane is 0.3 micron.

Preferably, the lower chamber is tapered with a large upper part and a small lower part. Thus, the collection of endometrial cells is more facilitated.

Preferably, the inner wall surfaces of the first flow guide pipe, the second flow guide pipe and the third flow guide pipe are provided with anticoagulant coatings. Thus, the blocking of the tube caused by the coagulation of menstrual blood in the flow guide tube can be prevented.

Because the menstrual blood contains a large amount of exfoliated endometrium, and the size of exfoliated endometrial tissue is different from that of stem cells, red blood cells and white blood cells, the method selectively separates the menstrual blood by using filter membranes with different pore diameters through a centrifugal separation and negative pressure drainage method so as to obtain the endometrial stem cells, the red blood cells, the white blood cells and the blood plasma in the menstrual blood, and the subsequent collection of the endometrial stem cells is easier.

When the endometrial cells are separated, the cotton fibers absorbing menstrual blood are placed in a filter pressing chamber, and then the menstrual blood is pressed and filtered out of the cotton fibers by two pressing plates of the filter pressing chamber. The menstrual blood is filtered by pressure and flows into the first centrifugal cylinder through the first guide pipe, the first centrifugal cylinder is driven by the first motor to rotate, and the menstrual blood flows into the first filter chamber outside the first centrifugal cylinder through the primary filter liquid of the first filter membrane under the action of centrifugal force. Then, the primary filtrate flows into a second centrifugal cylinder through a second guide pipe, the second centrifugal cylinder is driven by a second motor to rotate, and secondary filtrate passing through a second filter membrane flows into a second filter chamber outside the second centrifugal cylinder under the action of centrifugal force. Then, the secondary filtrate flows into the upper chamber through the third draft tube, under the negative pressure action of the negative pressure collection container, endometrial stem cells, red blood cells, white blood cells and plasma flow into the lower chamber through the third filter membrane and further flow into the negative pressure collection container filled with digestive juice, and then the separation of menstrual blood is completed. And subsequently centrifuging the mixed solution in the negative pressure collection container to collect the endometrial stem cells.

Compared with the prior art, the beneficial effects of the utility model reside in that: the utility model discloses a menstrual blood separator can directly carry out the selectivity to the menstrual blood collection appearance that contains absorbent material and separate, has reduced the contaminated probability of menstrual blood, and separation effect is good, can directly obtain endometrium stem cell, erythrocyte, leucocyte and plasma in the menstrual blood, has simplified follow-up work of collecting endometrium stem cell, has also improved the success rate of acquireing endometrium stem cell.

Drawings

Fig. 1 is a schematic structural view of the menstrual blood separator of the present invention.

In the figure, a filter pressing chamber 1, a first flow guide chamber 2, a first filter chamber 3, a second flow guide chamber 4, a second filter chamber 5, a third flow guide chamber 6, a third filter chamber 7, a pressure plate 8, a pneumatic telescopic rod 9, a control switch 10, a cover 11, a through hole 12, a first centrifugal cylinder 13, a first motor 14, a first filter membrane 15, a first flow guide pipe 16, a second centrifugal cylinder 17, a second motor 18, a second filter membrane 19, a second flow guide pipe 20, a third filter membrane 21, an upper chamber 22, a lower chamber 23, an output pipe 24, a negative pressure collection container 25, digestive juice 26 and a third flow guide pipe 27 are arranged.

Detailed Description

For better illustration of the objects, technical solutions and advantages of the present invention, the following description is further provided with reference to the accompanying drawings and examples. It should be understood that the examples are only for illustrating the technical effects of the present invention, and are not intended to limit the scope of the present invention.

Example 1

As shown in fig. 1, the present embodiment provides a menstrual blood separator, which includes a filter-pressing chamber 1, a first diversion chamber 2, a first filter chamber 3, a second diversion chamber 4, a second filter chamber 5, a third diversion chamber 6 and a third filter chamber 7, which are arranged in sequence from top to bottom. Specifically, two opposite pressing plates 8 are arranged inside the filter pressing chamber 1, a pneumatic telescopic rod 9 for driving the pressing plates 8 to horizontally reciprocate is arranged on the inner side wall surface of the filter pressing chamber 1, and a control switch 10 for controlling the pneumatic telescopic rod 9 to work is arranged on the outer side wall surface of the filter pressing chamber 1. In order to conveniently put the materials absorbed with menstrual blood into the press filtering chamber 1, the top of the press filtering chamber 1 is also provided with a cover 11. In order to filter and press menstrual blood on the material rapidly, the pressing plate 8 is also provided with through holes 12 which are distributed evenly. The first centrifugal cylinder 13 and the first motor 14 for driving the first centrifugal cylinder 13 to rotate are arranged in the first filtering chamber 3, the side wall of the first centrifugal cylinder 13 is provided with a first filtering membrane 15, and the size of the filtering holes of the first filtering membrane 15 is 50-60 micrometers. The first diversion chamber 2 is internally provided with a first diversion pipe 16 which is communicated with the filter-pressing chamber 1 and the first centrifugal cylinder 13. The second centrifugal cylinder 17 and a second motor 18 for driving the second centrifugal cylinder 17 to rotate are arranged in the second filtering chamber 5, a second filtering membrane 19 is arranged on the side wall of the second centrifugal cylinder 17, and the size of a filtering hole of the second filtering membrane 19 is 15-25 micrometers. A second guide pipe 20 which is communicated with the first filter chamber 3 and the second centrifugal cylinder 17 is arranged in the second guide chamber 4. The inside of the third filter chamber 7 is provided with a third filter membrane 21, and the size of the filter pores of the third filter membrane 21 is 0.3 micron. The third filter membrane 21 divides the third filter chamber 7 into an upper chamber 22 and a lower chamber 23, the lower chamber 23 is in a cone shape with a large upper part and a small lower part, the bottom of the lower chamber 23 is provided with an output pipe 24, the output pipe 24 is connected with a negative pressure collection container 25, and the negative pressure collection container 25 is filled with digestive juice 26. A third guide pipe 27 for communicating the second filter chamber 5 and the upper chamber 22 is arranged in the third guide chamber 6.

To prevent the clogging of the tubes caused by the coagulation of menstrual blood in the flow tubes, the inner wall surfaces of the first flow tube 16, the second flow tube 20 and the third flow tube 27 are provided with anticoagulant coatings.

Since the menstrual blood contains a large amount of exfoliated endometrium, and the size of exfoliated endometrial tissue is different from that of stem cells, red blood cells and white blood cells, in the embodiment, the menstrual blood is selectively separated by using filter membranes with different pore sizes through a centrifugal separation and negative pressure drainage method so as to obtain endometrial stem cells, red blood cells, white blood cells and plasma in the menstrual blood, so that the subsequent collection of endometrial stem cells is easier.

Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solutions of the present invention can be modified or replaced with equivalents without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. A menstrual blood separator is characterized by comprising a filter-pressing chamber, a first flow guide chamber, a first filter chamber, a second flow guide chamber, a second filter chamber, a third flow guide chamber and a third filter chamber which are arranged from top to bottom in sequence; two oppositely arranged press plates are arranged inside the filter pressing chamber, a pneumatic telescopic rod for driving the press plates to horizontally reciprocate is arranged on the inner side wall surface of the filter pressing chamber, and a control switch for controlling the pneumatic telescopic rod to work is arranged on the outer side wall surface of the filter pressing chamber; a first centrifugal cylinder and a first motor for driving the first centrifugal cylinder to rotate are arranged in the first filtering chamber, and a first filtering membrane is arranged on the side wall of the first centrifugal cylinder; a first guide pipe for communicating the filter-pressing chamber and the first centrifugal cylinder is arranged in the first guide chamber; a second centrifugal cylinder and a second motor for driving the second centrifugal cylinder to rotate are arranged in the second filtering chamber, and a second filtering membrane is arranged on the side wall of the second centrifugal cylinder; a second guide pipe for communicating the first filter chamber with the second centrifugal cylinder is arranged in the second guide chamber; a third filter membrane is arranged in the third filter chamber and divides the third filter chamber into an upper chamber and a lower chamber, an output pipe is arranged at the bottom of the lower chamber, a negative pressure collecting container is connected to the output pipe, and digestive juice is contained in the negative pressure collecting container; and a third flow guide pipe for communicating the second filter chamber with the upper chamber is arranged in the third flow guide chamber.

2. The menstrual blood separator according to claim 1, wherein a lid is provided on a top of the filter chamber.

3. Menstrual blood separator according to claim 1, wherein the pressure plate is provided with evenly distributed through holes.

4. The menstrual blood separator according to claim 1, wherein the first filter membrane has a pore size of 50 to 60 μm.

5. The menstrual blood separator according to claim 1, wherein the second filter membrane has a pore size of 15 to 25 μm.

6. The menstrual blood separator according to claim 1, wherein the filter pore size of said third filter membrane is 0.3 μm.

7. The menstrual blood separator according to claim 1, wherein said lower chamber has a tapered shape with a large upper portion and a small lower portion.

8. The menstrual blood separator according to claim 1, wherein an anticoagulant coating is provided on an inner wall surface of the first, second and third ducts.