JP2016140299A - Culture bag fixing block, and method for using culture bag fixing block - Google Patents

Abstract

PROBLEM TO BE SOLVED: To make it possible, in the case of culturing cells or the like by using a culture bag made of a soft packaging material, to culture the cells by making a bottom face of the culture bag flat state.SOLUTION: A culture bag fixing block that mounts thereon a culture bag made of a soft packaging material, in which culture solution is filled, and performs culturing of an object to be cultured comprises a mounting base for mounting the culture bag thereon, two or more fixtures are erected on a peripheral part of the mounting base in an opposing manner, a recess is formed on the fixtures. Two or more holes are provided for end parts of the culture bag, the culture bag is mounted on the mounting base of the culture bag fixing block, the fixtures of the culture bag fixing block are inserted into the holes to engage peripheral parts of the holes with the recesses of the fixtures, and the culture bag is fixed on the mounting base after a bottom face of the culture bag is made flat state.SELECTED DRAWING: Figure 2

Description

  The present invention relates to a culture technique for cells and the like, and more particularly to a culture bag fixing base used when cells and the like are cultured using a culture bag made of a soft wrapping material, and a method for using the culture bag fixing base.

In recent years, in the fields of pharmaceutical production, gene therapy, regenerative medicine, immunotherapy, etc., cells, tissues, microorganisms, viruses, etc. (hereinafter referred to as cells) are efficiently cultured in large quantities in an artificial environment. Is required.
In culturing such cells and the like, a culture bag made of a soft wrapping material can be suitably used. By using a culture bag made of a soft wrapping material, it is possible to impart excellent gas permeability to the culture bag, making it easy to construct a closed culture system. In addition, the capacity and area of the culture bag can be easily controlled, and it is easy to automate the feeding of a culture solution containing cells and culture medium (hereinafter referred to as culture solution). Benefits can be obtained.

On the other hand, when culturing with a culture bag placed on a gantry, wrinkles and slack are generated on the bottom surface of the culture bag simply by placing it, and a flat culture surface cannot be obtained. When wrinkles or slack are formed on the culture surface in this way, cells and the like are accumulated in the portion, resulting in excessive density and movement of the cells and the like are restricted, so that the proliferation efficiency of the cells and the like is lowered. It was.
In addition, such a place where cells or the like are accumulated has a problem that the cells or the like remain in the culture bag when the cultured cells or the like are collected.
Therefore, in order to solve such a problem, in the cell culture device described in Patent Document 1, holes are provided at the four corners of the culture bag, and fixtures are erected at the four corners of the gantry, and the gantry is provided at the hole of the culture bag. The culture surface is flattened by pulling the culture bag through the fixture.

International Publication No. 2008/136371 Pamphlet

However, even with this method, when the culture solution is in the culture bag, wrinkles and slack occur on the culture surface of the culture bag, and a place where cells and the like are accumulated in that portion. There was a problem that it decreased.
Specifically, in FIG. 5, for example, the culture bag 300 filled with the culture solution is placed on the gantry 200, and the fixture 100 is inserted into the hole 301 provided in the culture bag 300 to pull the culture bag 300. I have to. However, even if it did in this way, there existed a problem that a wrinkle and a slack arise in the bottom face of the culture bag 300. FIG.

Therefore, the present inventors provide a recess in a fixture standing on a gantry on which the culture bag is placed, engage a hole provided in the culture bag with the recess, and set the end of the culture bag at the height of the recess. By holding the part, the culture surface of the culture bag was successfully flattened, and the present invention was completed.
That is, the present invention provides a culture bag fixing base capable of culturing with a flat bottom surface of a culture bag and a culture bag fixing when culturing cells or the like using a culture bag made of a soft wrapping material. The purpose is to provide usage of the table.

  A culture bag fixing base according to the present invention is a culture bag fixing base for mounting a culture bag made of a soft wrapping material filled with a culture medium and culturing a culture object, and mounting the culture bag And two or more fixtures are erected on the periphery of the platform, and a recess is formed in the fixture.

  In addition, the method for using the culture bag fixing table according to the present invention includes two or more holes provided at an end of the culture bag, placing the culture bag on the frame of the culture bag fixing table, and A method of inserting a fixture of a bag fixing base into the hole, engaging a peripheral edge of the hole with a concave portion of the fixing tool, and fixing the culture bag to the gantry with a flat bottom surface of the culture bag It is as.

  According to the present invention, in the case of culturing cells or the like using a culture bag made of a soft wrapping material, the culture bag can be cultured with the bottom surface of the culture bag being flat.

It is a perspective view which shows the outline of the culture bag fixing base which concerns on 1st embodiment of this invention. It is a perspective view which shows the outline of the state which mounted the culture bag on the culture bag fixed base which concerns on 1st embodiment of this invention. It is a partial expanded side view which shows the outline of the state which mounted the culture bag on the culture bag fixed base which concerns on 1st embodiment of this invention. It is a partial expanded side view which shows the outline of the state which mounted the culture bag on the culture bag fixed base which concerns on 2nd embodiment of this invention. It is a partial expanded side view which shows the outline of the state of the culture bag mounted on the mount frame in the conventional cell culture.

  Hereinafter, preferred embodiments of the culture bag fixing base and the method for using the culture bag fixing base of the present invention will be described with reference to the drawings.

(First embodiment)
First, the culture bag fixing base according to the first embodiment of the present invention and a method for using the culture bag fixing base will be described with reference to FIGS. FIG. 1 is a perspective view showing an outline of a culture bag fixing base according to the present embodiment. FIG. 2 is a perspective view showing an outline of a state where the culture bag is placed on the culture bag fixing base according to the present embodiment, and FIG. 3 is a partially enlarged side view showing the outline of the state.

As shown in FIGS. 1 to 3, the culture bag fixing base 1 of the present embodiment includes a fixing tool 10 and a base 20 on which the fixing tool 10 is erected at four corners.
In the culture bag fixing base 1 of the present embodiment, the arrangement and the number of the fixing tools 10 are not limited to this, and it is sufficient that two or more fixing tools 10 are arranged facing the peripheral edge of the gantry 20. When a general culture bag 30 is used, the fixtures 10 are preferably erected at the four corners of the gantry 20, and when a relatively large culture bag is used, more fixtures 10 are erected. Is also preferable. Alternatively, one fixture 10 can be arranged at the center of the end of the short side of the culture bag fixing base 1, one at the center of the end of one short side, and two at the opposite end. It can also be arranged as described above.

  The shape of the fixture 10 is not particularly limited, but can be, for example, a substantially cylindrical shape. Moreover, the upper part of the recessed part 11 can also be made into a dome shape, and the lower part can also be made into a column shape or a prism shape.

  The fixture 10 is formed with a recess 11 for engaging a hole 31 provided in the culture bag 30. The recess 11 is preferably formed in the fixture 10 in a circumferential shape with respect to the horizontal plane, but is not limited to this, and is not limited to this. You may form in. Moreover, the horizontal cross section of the recessed part 11 is not limited to a circle, A polygon, a star shape, etc. may be sufficient.

  Further, in the fixture 10, the recess 11 has a thickness of 1 of the culture bag 30 upward from the position of the surface of the gantry 20 when the culture bag 30 filled with the culture solution is placed on the culture bag fixing table 1. The periphery of the hole 31 of the culture bag 30 is engageable at a position of / 2 (approximately 1/2 of the liquid depth d). The thickness of the culture bag 30 is the thickness when the culture solution is filled up.

The culture bag 30 is made of a soft packaging material, and has holes 31 at four corners thereof. Needless to say, the hole 31 is provided in an end region where the soft packaging material is heat-sealed, and the sealing performance in the culture bag 30 is not impaired by the hole 31.
Further, the arrangement and the number of the holes 31 are not limited to this, and it is preferable to provide the holes 31 corresponding to the fixtures 10 so that the fixtures 10 of the culture bag fixing base 1 can be inserted.

It is preferable that a part or all of the culture bag 30 has transparency so that the contents can be visually recognized. The culture bag 30 is required to have gas permeability (oxygen and carbon dioxide permeability) necessary for culturing cells and the like, and is used in a culture environment of 37 ° C. and 5% carbon dioxide concentration. It is preferable to do. Furthermore, the culture bag 30 preferably has low cytotoxicity, low elution property, and suitability for radiation sterilization in order to realize high proliferation efficiency of cells and the like.
Note that the culture object to be cultured using the culture bag 30 is not limited to cells, but can be any object that is cultured, such as microorganisms (including molds and yeasts) and viruses.

  As a material of the culture bag 30 that satisfies such conditions, a polyethylene resin is preferable. Examples of the polyethylene resin include polyethylene, a copolymer of ethylene and α-olefin, a copolymer of ethylene and vinyl acetate, an ionomer using ethylene, acrylic acid, a methacrylic acid copolymer, and a metal ion. Further, polyolefin, styrene elastomer, polyester thermoplastic elastomer, silicone thermoplastic elastomer, silicone resin and the like can also be used.

  The culture bag 30 is provided with a port for transferring contents to the outside, and a tube is connected to the port. The number of ports is not particularly limited, and one or more ports can be provided.

The four sides of the culture bag 30 can be sealed by heat sealing. Further, the culture bag 30 may be an integrally formed bag by blow molding.
Although the shape of the culture bag 30 and the shape of the accommodating part are not specifically limited, For example, it can be set as a rectangular shape. In addition, it is preferable to configure the shape of the accommodating portion of the culture bag 30 so that it gradually becomes narrower toward the port, because the residual of cells and the like during the transfer of the culture solution can be reduced.

The culture bag 30 is placed on the gantry 20 of the culture bag fixing base 1, the fixture 10 is inserted into the hole 31, and the periphery of the hole 31 is engaged with the recess 11 of the fixture 10. Thus, the end portion of the culture bag 30 is held at the height of the recess 11, and the culture bag 30 can be fixed to the gantry 20 with the bottom surface of the culture bag 30 in a flat state.
Then, such a culture bag 30 is filled with a culture solution containing cells and a medium to culture the cells and the like. Filling of the culture solution into the culture bag 30 may be before or after the culture bag 30 is fixed to the gantry 20.

  FIG. 3 shows that one end of the culture bag 30 is held at the height of the recess 11 of the fixture 10, but the end opposite to the end is also fixed in the same manner. It is held at the height of the recess 11 of the tool 10. The same applies to FIG. 4 described later.

According to the culture bag fixing table and the method of using the culture bag fixing table of this embodiment, the bottom surface of the culture bag 30 can be maintained in a flat state, and the culture surface is in a state free from wrinkles and slack. can do. For this reason, it becomes possible to maximize the culture area of the culture bag 30.
Moreover, by making the bottom surface of the culture bag 30 flat, cells and the like can be uniformly distributed (dispersed) in the culture bag 30. For this reason, it is possible to optimize the culture conditions in culturing cells and the like, and it is possible to further improve the proliferation efficiency of cells and the like.

(Second embodiment)
Next, the culture bag fixing base according to the second embodiment of the present invention and a method for using the culture bag fixing base will be described with reference to FIG. FIG. 4 is a partially enlarged side view showing an outline of a state in which the culture bag is placed on the culture bag fixing base according to the present embodiment.

As shown in FIG. 4, the culture bag fixing base 1 a of the present embodiment includes a fixing tool 10 a and a base 20 a in which the fixing tool 10 a is erected at four corners.
In the fixture 10a, when the culture bag 30 filled with the culture solution is placed on the culture bag fixing base 1a, the concave portion 11a is 3/4 of the thickness of the culture bag 30 upward from the position of the surface of the gantry 20a. The peripheral edge of the hole 31 of the culture bag 30 is provided to be engageable at a position (approximately 3/4 of the liquid depth d).

According to this embodiment, the culture bag 30 is placed on the gantry 20a of the culture bag fixing base 1a, the fixing tool 10a is inserted into the hole 31, and the peripheral edge of the hole 31 is inserted into the recess 11a of the fixing tool 10a. Can be engaged. Thus, the end portion of the culture bag 30 is pulled up and held at the height of the recess 11a, and the culture bag 30 can be fixed to the gantry 20a with the bottom surface of the culture bag 30 in a flat state.
Therefore, according to this embodiment, the bottom surface of the culture bag 30 can be maintained in a flat state, the culture surface can be free of wrinkles and slack, the culture area can be maximized, etc. It is possible to obtain the same effect as that of the first embodiment.

Thus, according to the present embodiment, the bottom surface of the culture bag 30 can be made flat by pulling up the opposing end portions of the culture bag 30 to the height of the recess 11a. Such an effect can be similarly obtained even when the position of the recess 11a is above the position of the surface of the gantry 20a and slightly above the thickness of the culture bag 30.
From such a viewpoint, it is preferable that the position where the concave portion is formed in the fixture is in the range of 1/2 to 3/2 of the thickness of the culture bag filled with the culture solution from the position of the surface of the gantry. The range of 1/2 to 1 is more preferable, and the range of 1/2 to 3/4 is more preferable.

  Other points such as the arrangement, number, and shape of the fixture 10a in this embodiment, the shape of the recess 11a, and the characteristics of the culture bag 30 can be the same as those in the first embodiment.

While the present invention has been described with reference to the preferred embodiment, it is needless to say that the present invention is not limited to the above-described embodiment, and various modifications can be made within the scope of the present invention.
For example, the shape of the fixture is not limited to the embodiment, and other shapes such as a substantially rectangular parallelepiped may be used as long as the same effects as the embodiment can be obtained by providing the recesses. In addition, it is possible to make appropriate changes such as allowing the culture solution in the culture bag to be stirred by vibrating the gantry.

  The present invention can be suitably used in gene therapy, immunotherapy, regenerative medicine, antibody drug production, etc., in which cells are cultured in large quantities in a closed system using a culture container.

DESCRIPTION OF SYMBOLS 1,1a Culture bag fixing stand 10,10a Fixture 11,11a Recess 20,20a Mount 30 Culture bag 31 hole

Claims (6)

A culture bag fixing stand for culturing a culture object by placing a culture bag made of a soft wrapping material filled with a culture solution,
A culture bag fixing base comprising a base for placing a culture bag, wherein two or more fixtures are erected on the periphery of the base, and a recess is formed in the fixture.   The concave portion is formed at a position 1/2 to 3/2 of the thickness of the culture bag filled with a culture solution, upward from the position of the surface of the gantry in the fixture. The culture bag fixing table according to Item 1.   The culture bag fixing base according to claim 1 or 2, wherein the concave portion is formed in the fixture in a circumferential shape with respect to a horizontal plane.   The culture bag fixing base according to any one of claims 1 to 3, wherein the fixture is erected at four corners of the gantry.   Two or more holes are provided in the end portion of the culture bag corresponding to the fixing tool, and the fixing tool is inserted into the hole to engage the peripheral edge of the hole with the recess. The culture bag fixing base in any one of claim | item 1 -4. Two or more holes are provided in an end portion of the culture bag, the culture bag is placed on the culture bag fixing base according to any one of claims 1 to 5, and the culture bag fixing base fixture A culture bag, wherein the culture bag is fixed to the pedestal with the periphery of the hole being engaged with the recess of the fixture, the bottom surface of the culture bag being flat, How to use the fixed base.

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