KR101626563B1 - A Assembly To Hold The Culture Bag - Google Patents

Abstract

Disclosed is a culture bag storage assembly which is easy to move, store and load the culture bag. A culture bag storage assembly according to the present invention includes a culture bag having a storage portion capable of containing a culture liquid therein and a rack for accommodating the culture bag, wherein the culture bag has a plurality of first air holes A first case formed with the first case; A second case having a plurality of second vent holes formed in an upper portion of the first case and covering the culture bag; And a fastening portion for fastening the first case and the second case.
According to the present invention, the culture bag can be safely moved by attaching the culture bag to the rack, and it is possible to facilitate storage and loading in the incubator by forming the lamination protrusions and the corresponding protrusions, It can help to cultivate uniformly. The gas permeable film used in the production of the culture bag and the first and the second air holes formed in the rack smoothly supply the oxygen required for cell growth and smoothly discharge the harmful gas inside the culture bag to the outside, Cells and the like can be maintained in a healthy state for a long time, and the culture efficiency can be improved, thereby contributing to the mass production of the cells themselves or the products produced by the cells.

Description

[0001] The present invention relates to a culture bag holding assembly,

The present invention relates to a culture bag retention assembly, and more particularly, to a culture bag retention assembly, in which a culture bag is placed in a rack accommodating the culture bag to secure the movement of the culture bag and to store the culture bag for easy storage and loading in the incubator Assembly.

A culture solution in which a plant, a bacterium, a cultured cell and a nutrient required therefor is mixed is accommodated in various containers such as a flask, a plate, a plate, a culture bag and the like depending on the purpose or object of propagation. Among them, the encapsulated culture bag is suitable for culturing immune cells and the like because of excellent shielding effect against the outer world and easy maintenance of cleanliness.

Such culture pouches are being developed in various ways according to the development of biotechnology. Korean Patent Laid-Open No. 10-2010-0065861 discloses a method for introducing air filtered by foreign substances such as bacteria and discharging harmful gas therein smoothly The present invention relates to an invention for a 'culture bag for culture of seeds'.

Specifically, in the background art, a rectangular opening 12 is formed in each of the front and rear bag bodies 10, and an air inlet 20 composed of the ventilating filter 20 and the outer shell 30 I and a gas exhaust portion O composed of a ventilation filter 20 and an enclosure 30 provided on the inner side are provided, respectively.

The above-mentioned background art only describes the technology of the culture liquid bag itself and does not mention at all the apparatus that can safely and effectively treat the culture liquid bag.

The safe and effective treatment of the culture bag is not only convenient for the operator but also contributes to cultivation of germs and cells uniformly and contributes to mass production, , Agriculture, medical science, etc., which have been continuously developing.

(0001) Korean Patent Publication No. 10-2010-0065861 (published on June 17, 2010)

SUMMARY OF THE INVENTION An object of the present invention is to provide a culture bag storage assembly that enables a safe movement of a culture bag by placing the culture bag in a rack accommodating the culture bag and facilitates storage and loading in the incubator.

The object of the present invention is achieved by a method for producing a culture bag comprising a first bag having a culture bag and a rack accommodating the culture bag, the bag having a receptacle for storing the culture liquid therein and having a plurality of first vent holes; A second case having a plurality of second vent holes formed in an upper portion of the first case and covering the culture bag; And a fastening portion for fastening the first case and the second case to each other.

Wherein the culture bag comprises: a body having a containing portion capable of containing a culture liquid therein; A pair of tubes connected to the receptacle so as to allow the culture fluid to flow in and out of the body, the tubes being projected outward from one side of the body; And a cap provided at one end of the tube facing the receiving portion to open and close the tube.

The body may further include a labeling unit formed on one side of the frame.

The body may be formed by laminating a pair of gas permeable films and fusing the rims.

And a hinge unit connecting the first case and the second case so that the first case and the second case can rotate relative to each other.

The first case further includes a plurality of mounting protrusions formed upwardly protruding from a periphery where a plurality of first ventilation holes are formed, wherein the culture bag has an air hole formed at a position corresponding to the plurality of mounting protrusions, .

The second case may further include a plurality of seating portions engageable with the seating projection ends on the bottom surface.

A plurality of lamination protrusions are formed on one of the first case bottom surface and the second case upper surface and a plurality of lamination grooves are formed on the other of the first case bottom surface and the second case upper surface so as to be engageable with the lamination protrusions, have.

At least one of the outer surface of the lamination protrusion and the inner side of the lamination groove may be formed with a step so as to separate the culture bag storage assemblies stacked up and down.

A plurality of lamination protrusions are formed on one of the first case bottom surface and the second case upper surface and a plurality of corresponding protrusions are formed on the other of the first case bottom surface and the second case upper surface, .

The first case may further include a support portion which is rotatable at a predetermined angle on a lower surface to support the first case.

The supporting portion may include a pair of supporting rods symmetrically formed along an edge of the lower surface of the first case and having one end connected to one side of the first case and an edge of the lower surface of the first case, A pedestal including a linkage formed along the guide rail; A receiving groove formed in the bottom surface of the first case so that the pedestal is received and coplanar with the bottom surface of the first case; A pair of support portions formed at one ends of the support so as to be able to rotate relative to the first case; And a draw-out groove formed at one side of the receiving groove to facilitate drawing out of the pedestal.

According to the present invention, the culture bag can be safely moved by attaching the culture bag to the rack, and it is possible to facilitate storage and loading in the incubator by forming lamination protrusions and corresponding protrusions, It can help to cultivate uniformly.

The gas permeable film used in the production of the culture bag and the first and the second air holes formed in the rack smoothly supply the oxygen required for cell growth and smoothly discharge the harmful gas inside the culture bag to the outside, Cells and the like can be maintained in a healthy state for a long time, and the culture efficiency can be improved, thereby contributing to the mass production of the cells themselves or the products produced by the cells.

1 is a perspective view of a culture bag storage assembly according to the present invention.
FIG. 2 is an exploded perspective view of a culture bag storage assembly according to the present invention.
3 is a plan view of a culture bag storage assembly according to the present invention.
4 is a bottom view of the culture bag storage assembly according to the present invention.
5 is a side view showing a state in which the culture bag storage assembly according to the present invention is stacked up and down.
6 is a perspective view showing a lamination protrusion and a lamination groove of a culture bag storage assembly according to the present invention.
7 is a perspective view showing the use state of the pedestal according to the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. In the following description of the present invention, the well-known functions or constructions are not described in order to simplify the gist of the present invention.

FIG. 1 is a perspective view showing a culture bag storage assembly according to the present invention, FIG. 2 is an exploded perspective view of a culture bag storage assembly according to the present invention, FIG. 3 is a plan view of a culture bag storage assembly according to the present invention, 4 is a bottom view of the culture bag storage assembly according to the present invention, FIG. 5 is a side view showing a state in which the culture bag storage assembly according to the present invention is stacked up and down, FIG. 6 is a cross- And FIG. 7 is a perspective view showing the use state of the pedestal according to the present invention.

1 to 4, the culture bag storage assembly 100 according to the present invention may include a culture bag 110 and a rack 120 for accommodating the culture bag 110. As shown in FIG.

(Culture bag)

The culture bag 110 is a constituent element for selectively blocking the contact with the external environment by placing a culture solution in which a growth material such as a plant, a bacterium, a cell, etc. and a nutrient required thereon is mixed, A body 112, a tube 114, a stop 116, and a labeling portion 118.

Specifically, the body 112 is a constituent element provided with a receiving portion 111 capable of receiving a culture liquid therein, and any shape or material may be used as long as it can contain a culture liquid, which is a fluid substance, .

2, the body 112 according to the embodiment of the present invention is formed in a rectangular shape as a whole and protrudes up and down at the center to form a receiving portion 111. The rim has a flat plate shape, And a converging portion 111a narrowing in width toward one side (front side) is formed. In the plate-shaped rim portion, a plurality of air-through holes 113 are formed and sandwiched by a plurality of seating projections 134 to be described later.

The body 112 is formed by superimposing a pair of gas permeable films, which are functional materials, and then fusing the edges. In this case, the gas permeable film is manufactured by mixing polyethylene (PE), a thermoplastic plastic, and acryl, and it is possible to prevent a decrease in oxygen concentration in the accommodating portion 111 even when a large number of cells are cultured, It helps to improve.

The tube 114 is a tubular component provided at one side of the body 112 to allow the culture fluid to flow in and out of the body 112. The tube 114 may have any cross section as long as both ends are communicable.

The tube 114 according to the embodiment of the present invention is formed such that one end thereof is connected to the receiving portion 111 and the other end is protruded outside the body 112 in the vicinity of the converging portion 111a.

The tubes 114 may be formed in such a manner that the pair of the tubes 114 are symmetrically arranged and then fused together with the step of molding the body 112 described above.

The reason for including the pair of tubes 114 here is to properly remove bubbles generated when the culture liquid is injected into the accommodating portion 111 through one tube 114. Therefore, the operator can remove the bubbles generated in the other tube 114 while injecting the culture liquid into any one of the tubes 114.

On the other hand, when the culture liquid contained in the accommodating portion 111 is discharged to the outside with the pair of tubes 114 facing the ground, the shape of the converging portion 111a having a narrow width is such that the culture liquid is supplied to the pair of tubes 114 ), So that the culture liquid can be neatly and easily discharged to the outside.

The cap 116 is a component for opening and closing the tube 114 and is provided at one end of the tube 114 facing the receiving portion 111.

The stopper 116 according to the embodiment of the present invention is formed in the form of a lid of a silicone material that surrounds the end portion of the tube 114 to prevent leakage of the culture liquid. The silicone material stopper 116 allows a nutrient material or the like to pass through the stopper 116 by using a syringe or the like or to introduce the nutrient into the receiver 111 or to take out the culture liquid to the outside.

The labeling unit 118 is a component provided to record basic information on a culture liquid or the like. When the culture bag 110 is placed in the rack 120, ).

However, the labeling unit 118 according to the embodiment of the present invention is formed to protrude outward at a position adjacent to the converging unit 111a where the width of the body 112 is narrowed. As a result, the operator can easily confirm the basic information about the culture liquid from the outside, so that the work related to the culture liquid can be efficiently performed.

(Rack)

The rack 120 is a component that receives the above-described culture bag 110 to facilitate safe transportation and storage and loading of the culture bag 110, and prevents damage to the culture surface of the adhered cell in the culture bag 110, A first case 130, a second case 140, and a fastening part 150. Here, since the detailed components of the first case 130 and the detailed components of the second case 140 are mutually symmetric, they can be applied mutually.

The first case 130 is provided with a culture bag 110 on its upper surface so that the culture bag 110 is not separated from the rack 120 when it is moved and the lower surface of the culture bag 110 formed of a gas- To be in contact with the air.

The first case 130 according to the embodiment of the present invention may have a rectangular shape corresponding to the entire shape of the culture bag 110 described above. And is formed so as to have a narrow width corresponding to the converging portion 111a. This is to increase the space utilization of the culture bag storage assembly 100 of the present invention to improve the efficiency of stacking and to more easily recover the culture result in the culture bag 110 upon completion of the culture.

Specifically, the first case 130 may include a first vent hole 132, a seating projection 134, and a corresponding projection 136.

The first vent hole 132 is formed at the center of the first case 130 so that the lower surface of the culture bag 110 can contact the air. .

However, the plurality of first vent holes 132 according to the embodiment of the present invention are formed at the center except for the edge of the first case 130 in the form of a lattice of the same size. This makes it possible for the lower surface of the gas permeable culture bag 110 to contact with the air through the first vent hole 132 at a constant area so that the culture material such as cells can be uniformly cultured on the entire lower surface of the culture bag 110 And the cell is maintained in good condition for a long period of time, so that a better culture result can be obtained. Thus, for this purpose, the size, number, and arrangement of the first vent holes 132 may vary.

The seating protrusion 134 is a component that is inserted in the above-described air hole 113 to prevent the culture bag 110 from being detached from the rack 120 during movement and loading. As shown in FIG.

The projecting length, width, arrangement and number of the mounting projections 134 can be variously modified within a range that does not move the rack 120 from the rack 120 and move within the rack 120 when the rack 120 is moved .

However, the seating protrusion 134 according to the embodiment of the present invention is not limited to the five corners of the first case 130, which are symmetrical with respect to the longitudinal direction of the culture bag 110 (direction parallel to the tube of FIG. 2) So that the load is not concentrated on either side.

The protrusion length and width are formed in a length and a width so as to prevent the culture bag 110 from moving in the rack 120 in consideration of the thickness of the culture bag 110 and the size of the air hole 113.

On the other hand, the seat cushion 134 may be tapered inward toward the upper end so as to be easily fitted into the seat cushion 113.

3 to 6, the corresponding protrusions 136 and the lamination protrusions 146 are spaced apart and secured between the upper and lower culture bag storage assemblies 100 through mutual engagement, Assembly 100 to allow circulation thereof.

If the protrusions 136 and the lamination protrusions 146 have protruding lengths, shapes, arrangements, and engaging structures capable of mutually engaging and fixing the culture bag storage assemblies 100 at regular intervals, Of course.

However, the corresponding protrusions 136 according to the embodiment of the present invention are symmetrically formed at the four corners of the bottom surface of the first case 130, and are protruded in a circular arc shape. The lamination protrusions 146 are formed to protrude in an arc shape slightly inclined inward from the upper surface of the second case 140 so as to be fit-fit so as to correspond to the protrusion length, shape, and arrangement of the corresponding protrusions 136, respectively.

As a result, the culture bag storage assemblies 100 stacked up and down are separated from each other by the protruding length of the lamination protrusions 146 (or the corresponding protrusions 136), air circulation occurs between them, The upper and lower surfaces can be smoothly brought into contact with the air. This contributes to maintenance of cell quality and improvement of culture efficiency in the culture medium.

On the other hand, the positions of the corresponding projections 136 and the stacked projections 148 are formed such that the corresponding projections 136 are formed on the upper surface of the second case 140 and the stacked projections 146 are formed on the first case 130, It may be formed on the bottom surface.

6 is a modification of the corresponding protrusion 136 and the lamination protrusion 146 according to the embodiment of the present invention. The lamination protrusion 246 is formed in a ring shape at five corners of the upper surface of the second case 140 Respectively. At this time, the lamination protrusion 246 is formed in the vertical upper portion of the above-described seating protrusion 134, and the lamination groove 134 is formed in the vertical lower portion of the seating protrusion 134. [

The stacking protrusions 246, the seating protrusions 134 and the lamination grooves 236 are positioned in this order from the top, and the culture bag storage assembly 100 is stacked up and down The stacking protrusion 246 of the lower culture bag storage assembly 100 is engaged with the stacking groove 236 of the upper culture bag storage assembly 100.

The vertical structure of the stacked protrusions 246, the seating protrusions 134 and the laminated grooves 236 makes the stacking of the culture bag storage assembly 100 robust and allows the placement of the culture bag 110 through the seating protrusions 134 In addition, it is possible to stably support the load of the culture bag storage assembly 100 mounted on the upper side.

The step 247 may be formed on at least one of the outer circumferential surface of the lamination protrusion 246 and the inside of the lamination groove 236. [ The step 247 separates the stacked culture bag storage assemblies 100 stacked up and down such that the stacked protrusions 246 are not completely inserted into the lamination grooves 236.

As a result, the upper and lower culture bag storage assemblies 100 are spaced apart from each other by the height of the step 247, and air circulation occurs therebetween, so that the upper and lower surfaces of the loaded culture bag 110 are smoothly contacted with air . This contributes to the maintenance of the quality of the cells in the culture liquid and the improvement of the culture efficiency as well as the lamination protrusions 146 (or the corresponding protrusions 136).

The lamination protrusions 246 and the lamination grooves 236 are formed on the upper surface of the second case 140 and the lamination protrusions 246 are formed on the upper surface of the first case 130, It may be formed on the bottom surface.

1 to 3, the second case 140 is configured such that the first case 130 supports the lower surface of the culture bag 110, and the upper surface of the culture bag 110 is paired with the second case 140 The second case 140 may include a second vent hole 142, a seating portion 144, and a stacking protrusion 146. [

Since the second case 140 has a technical configuration substantially the same as the first case 130 described above, only the differences will be described below.

The seating part 144 is provided on the lower surface of the second case 140 so that the culture bag 110 can be firmly fixed to the rack 120. The seating part 144 is positioned at a position corresponding to the end of the seating projection 134 of the first case 130 And can be coupled with each other.

At this time, the coupling structure may be variously modified. In the embodiment of the present invention, the seating portion 144 is formed of a ring-shaped projection, and the end portion of the seating projection 134 engages inwardly in a ring shape. The seating portion 144 may be formed in the first case 130 and the seating protrusion 134 may be formed in the second case 140.

Meanwhile, a mounting hole 148 may be formed on one side of the edge of the second case 140 so that the culture bag storage assembly 100 can be hooked to a ring or the like.

The fastening part 150 is a component for fixing the first case 130 and the second case 140 to each other and has a shape corresponding to one side of each of the first case 130 and the second case 140 Various fastening devices having a coupling structure can be applied.

In the fastening part 150 of the embodiment of the present invention, a ring-shaped ring is formed on one side of the first case 130, and a hook-shaped fastening body that can be fastened is formed on one side of the second case 140.

The hinge unit 160 is provided to facilitate mounting of the culture bag 110. The hinge unit 160 includes a first case 130 and a second case 140 so that the first case 130 and the second case 140 can rotate relative to each other. And the second case 140, respectively.

At this time, the hinge unit 160 is preferably formed at a position opposite to the forming position of the fastening unit 150 so that the hinge unit 160 can smoothly rotate without interference with the fastening unit 150 described above.

2, 4, and 7, the receiving unit 170 may be configured to insert nutrients or the like from the plug 116 of the culture bag 110 by using a syringe or the like, or to draw out the culture liquid to the outside It is a constituent element provided for facilitating.

The support portion 170 may be provided on one of the first and second cases 130 and 140 so that the stopper 116 of the culture bag 110 can support the upward or sideways state. It is also possible to have a structure of.

However, the receiving part 170 according to the embodiment of the present invention is provided on the bottom surface of the first case 130 (the upper surface of the second case 140 is also not possible) So that the first case 130 can be supported.

Specifically, the receiving portion 170 may include a pedestal 174, a receiving groove 176, a turning portion 178, and a receiving groove 179.

The pedestal 174 is a constituent element of a 'C' shape as a whole, and is composed of a pair of supports 172 and a connecting rod 173. The pair of supports is formed symmetrically along the edge of the lower surface of the first case 130 and one end is connected to one side of the lower surface of the first case 130. The connection unit 173 includes a pair of supports 172, And is formed along the edge of the lower surface of the first case 130.

The receiving groove 176 is formed in the lower surface of the first case 130 to be concave so that the pedestal 174 can be received and coplanar with the lower surface of the first case 130.

The pivot portion 178 interconnects the pair of supports 172 and the first case 130 and at the same time supports the pair of supports 172 so as to be rotatable relative to the first case 130 ).

The pivoting unit 178 is configured to stop the pivoting operation at predetermined angles (stoppoles and latching protrusions) on the inner side of the one end of the support table 172 and the first case 130 engaged with the one end of the support table 172, have.

The draw-out groove 179 can be recessed from one side of the receiving groove 176 so as to facilitate the drawing of the pedestal 174 and the operator's convenience.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, It is obvious to those who have. Accordingly, such modifications or variations should not be individually understood from the technical spirit and viewpoint of the present invention, and modified embodiments should be included in the claims of the present invention.

100: culture bag storage assembly 110: culture bag
111: receptacle 112: body
113: opening of the hole 114: tube
116: cap 118: labeling section
120: rack 130: first case
132: first vent hole 134: seating projection
136: corresponding projection 140: second case
142: second vent hole 144: seat part
146: laminated projection 150:
160: hinge portion 170:
172: support 173: connecting rod
174: pedestal 176: storage groove
178: turning portion 179: drawing groove
236: Lamination groove 246: Lamination projection
247:

Claims (12)

A culture bag having a storage portion capable of containing a culture liquid therein, and a rack for accommodating the culture bag,
Wherein,
A first case on which the culture bag is placed and on which a plurality of first ventilation holes are formed;
A second case disposed above the first case and covering the culture bag and having a plurality of second ventilation holes; And
And a fastening portion for fastening the first case and the second case,
Wherein the first case further includes a plurality of seating projections formed upwardly projecting from a periphery where a plurality of the first ventilation holes are formed,
Wherein the culture bag is provided with an air hole at a position corresponding to a plurality of the seating projections and is fitted in the seating projections,
The second case further includes a plurality of seating portions engageable with the seating projection ends on the bottom surface,
Wherein a plurality of lamination protrusions are formed on one of the first case bottom surface and the second case upper surface and a plurality of lamination grooves which are engageable with the lamination protrusions are formed on the other one of the lamination protrusions, Can be loaded,
Wherein at least one of the outer circumferential surface of the lamination projection and the inner side of the lamination groove forms a step so as to separate the culture bag storage assemblies stacked up and down.
A culture bag having a storage portion capable of containing a culture liquid therein, and a rack for accommodating the culture bag,
Wherein,
A first case on which the culture bag is placed and on which a plurality of first ventilation holes are formed;
A second case disposed above the first case and covering the culture bag and having a plurality of second ventilation holes; And
And a fastening portion for fastening the first case and the second case,
Wherein the first case further includes a plurality of seating projections formed upwardly projecting from a periphery where a plurality of the first ventilation holes are formed,
Wherein the culture bag is provided with an air hole at a position corresponding to a plurality of the seating projections and is fitted in the seating projections,
The second case further includes a plurality of seating portions engageable with the seating projection ends on the bottom surface,
A plurality of lamination protrusions are formed on one of the first case bottom surface and the second case upper surface and a plurality of corresponding protrusions are formed on the other of the first case bottom surface and the second case upper surface, Thereby separating the assemblies from each other.
3. The method according to claim 1 or 2,
The culture bag may contain,
A body having a receiving portion capable of receiving a culture liquid therein;
A pair of tubes connected to the receptacle so as to allow the culture liquid to flow in and out of the body, the tubes being projected outward from one side of the body;
And a cap provided at one end of the tube facing the receiving part to open and close the tube.
The method of claim 3,
Wherein the body further comprises a labeling portion formed at one side of the rim.
The method of claim 3,
Wherein the body is formed by laminating a pair of gas permeable films and fusing the rims.
3. The method according to claim 1 or 2,
Further comprising a hinge portion connecting the first case and the second case so that the first case and the second case can rotate relative to each other.
3. The method according to claim 1 or 2,
Wherein the first case further comprises a support portion which is pivotally mounted on a lower surface at a predetermined angle to support the first case.
8. The method of claim 7,
[0030]
A pair of supports formed symmetrically with respect to an edge of the lower surface of the first case and having one end connected to one side of the first case, an edge of the lower surface of the first case, A pedestal including a linkage formed along the guide rail;
A receiving groove formed in the bottom surface of the first case so that the pedestal is received and coplanar with the bottom surface of the first case;
A pair of support portions formed at one end of the support so as to be able to rotate relative to the first case;
And a draw-out groove formed at one side of the receiving groove to facilitate the drawing of the pedestal.
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