CN114308177A - Cell harvesting bracket for cell culture bag and use method - Google Patents
Cell harvesting bracket for cell culture bag and use method Download PDFInfo
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- CN114308177A CN114308177A CN202111653882.8A CN202111653882A CN114308177A CN 114308177 A CN114308177 A CN 114308177A CN 202111653882 A CN202111653882 A CN 202111653882A CN 114308177 A CN114308177 A CN 114308177A
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Abstract
The invention belongs to the technical field of cell harvesting, in particular to a cell harvesting bracket for a cell culture bag and a using method thereof, the cell harvesting bracket comprises a detachable mounting base plate, a lateral baffle, a suspension bracket, a dual-mode multi-point culture bag clamping mechanism, a vacuum adsorption component, a conduit control mechanism and a weighing machine, the lateral baffle is welded on one side of the top of the detachable mounting base plate, the suspension bracket is welded on the top of the lateral baffle, the two dual-mode multi-point culture bag clamping mechanisms are respectively arranged at two ends of the top of the suspension bracket and are used for clamping and fixing the cell culture bag and helping to harvest the cell, the vacuum adsorption component is arranged on the detachable mounting base plate in a penetrating way and used for adsorbing a smooth desktop, the conduit control mechanism is fixedly arranged on the lateral baffle, and the conduit control mechanism is arranged at the bottom of the dual-mode multi-point culture bag clamping mechanism, the cell harvesting bracket is simple to use and has strong functionality, need not the touching again after will cultivateing the bag fixed, intelligent degree is high, and the cleanliness factor is higher.
Description
Technical Field
The invention belongs to the technical field of cell harvesting, and particularly relates to a cell harvesting bracket for a cell culture bag and a using method of the cell harvesting bracket.
Background
The epidemic cell treatment technology is rapidly developed, and attracts attention as a fourth tumor treatment means after surgery, radiotherapy and chemotherapy, the cell harvesting mode at the present stage is single, most of the cell harvesting methods need to be operated by hands of a laboratory technician, and although part of the cell harvesting methods also adopt a mechanical structure, the cell harvesting methods are simple in structure, convenient to use, poor in functionality, low in intelligence degree and low in cleanliness degree, so that the cell harvesting support for the cell culture bag and the using method are provided.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a cell harvesting bracket for a cell culture bag and a using method thereof, and the cell harvesting bracket has the characteristics of simple use, strong functionality, high intelligent degree and high cleanliness.
(II) technical scheme
In order to achieve the purpose, the invention provides the following technical scheme: a cell harvesting bracket for a cell culture bag comprises a detachable mounting base plate, a lateral baffle, a suspension bracket, a dual-mode multi-point position culture bag clamping mechanism, a vacuum adsorption component, a conduit control mechanism and a weighing machine;
the side baffle is welded on one side of the top of the detachable mounting base plate, the suspension bracket is welded on the top of the side baffle, and the two dual-mode multi-point culture bag clamping mechanisms are respectively arranged at two ends of the top of the suspension bracket and are used for clamping and fixing a cell culture bag and assisting in cell harvesting;
the vacuum adsorption component is arranged on the detachable mounting base plate in a penetrating mode and used for adsorbing a smooth desktop;
the conduit control mechanism is fixedly arranged on the lateral baffle, is positioned at the bottom of the dual-mode multi-point-position culture bag clamping mechanism and is used for clamping a liquid outlet hose of a culture bag and controlling liquid outlet through clamping and releasing;
the weighing device is arranged at the top of the detachable installation chassis and used for weighing the container, and the weighing device is positioned at the bottom of the dual-mode multi-point position culture bag clamping mechanism.
Preferably, the vacuum adsorption component comprises a vacuum pump, an air suction pipe and an adsorption disc, the air suction pipe is fixedly arranged in the detachable installation base plate in a penetrating mode, the vacuum pump is arranged at the top of the detachable installation base plate, the air suction end of the vacuum pump is communicated with the air suction pipe, and the adsorption disc is fixedly arranged at the bottom of the air suction pipe.
Through adopting above-mentioned scheme for the vacuum pump can breathe in through the breathing pipe, and then will be located the air of bottom adsorption disk and take out, and the in-service use in-process is with adsorption disk and smooth desktop contact back moderate degree extrusion, opens the vacuum pump again and makes the air in the adsorption disk taken out and form the negative pressure, utilizes the negative pressure to fix this support on the desktop, and easy operation is convenient, and is fixed effectual, and convenient the dismantlement.
Preferably, pipe control mechanism includes Z type support, ring frame, miniature telescopic link and cut-off plate, the ring frame welding just is located the one end of Z type support the bottom of bag fixture is cultivateed to dual mode multiple spot position, the fixed setting of miniature telescopic link is in the interior border side of ring frame, the cut-off plate welding is in on the flexible end of miniature telescopic link, the play liquid hose of cultivateing the bag wear to establish ring frame with in the logical inslot between the cut-off plate.
By adopting the scheme, after the culture bag is clamped and fixed by the double-mode multi-point-position culture bag clamping mechanism, the liquid outlet hose of the culture bag is arranged in the through groove between the annular frame and the shutoff plate in a penetrating manner, then the shutoff plate is driven to move by controlling the expansion and contraction of the miniature telescopic rod, the liquid outlet hose of the culture bag is extruded by utilizing the extrusion effect of the shutoff plate to realize the shutoff, and the shutoff plate is retracted when the liquid is required to be discharged.
Preferably, the double-mode multi-point position culture bag clamping mechanism comprises an adjusting motor, a vertical mounting plate, an upper telescopic rod, a lower telescopic rod, a conical extrusion fixing rod, an extrusion fixing strip, a longitudinal adjusting telescopic rod, a transverse extrusion telescopic rod and an extrusion plate, wherein the adjusting motor is mounted on the suspension bracket through a motor base.
By adopting the scheme, the adjusting motor is in a fixed state, and simultaneously has a driving effect, so that the culture bag clamped in the double-mode multi-point-position culture bag clamping mechanism is overturned, and the cell sap is discharged.
Preferably, the central point of perpendicular mounting panel put with adjusting motor's output shaft is connected, go up the telescopic link with down the telescopic link is installed respectively top one side and bottom one side of perpendicular mounting panel, go up the telescopic link with the quantity of telescopic link is two down, singly it is fixed to go up the telescopic link the toper extrusion dead lever, the extrusion fixed strip welding is two between the flexible end of telescopic link down.
Through adopting above-mentioned scheme for it is flexible that lower telescopic link control extrusion fixed strip, the bottom position that utilizes two double mode multiple spot position to cultivate bag fixture's extrusion fixed strip carries out the centre gripping to the bottom position of cultivateing the bag and fixes, goes up telescopic link control toper extrusion dead lever simultaneously and stretches out, utilizes two double mode multiple spot position to cultivate bag fixture's toper extrusion dead lever to realize the fixed to cultivation bag top both sides position.
Preferably, vertically adjust the fixed setting of telescopic link one side of perpendicular mounting panel just vertically adjust the flexible end of telescopic link and move towards two perpendicularly go up the mid point between the telescopic link, transversely extrude the telescopic link vertical fixation and be in vertically adjust the flexible of telescopic link and serve, the stripper plate welding is in transversely extrude the flexible of telescopic link and serve, the one end of stripper plate is the fillet structure.
Through adopting above-mentioned scheme for vertically adjust the telescopic link control lateral extrusion telescopic link and the stripper plate and remove, utilize the lateral extrusion telescopic link control stripper plate to remove and realize the extrusion effect, utilize extrusion and removal between two stripper plates to realize the extrusion discharge to residual liquid in the cell culture bag during in-service use, improve the functionality.
A method of using a cell harvesting scaffold for a cell culture bag, comprising the steps of:
s1, wiping the tabletop to be used, ensuring that excessive dust and impurities do not exist, then placing the detachable mounting base plate in a direction convenient to use, then pressing the detachable mounting base plate and the vacuum adsorption component downwards appropriately, starting the vacuum pump while pressing to enable air in the adsorption plate to be pumped out to form negative pressure, and fixing the detachable mounting base plate on the tabletop by utilizing the negative pressure;
s2, placing a culture bag needing cell harvesting between two dual-mode multi-point culture bag clamping mechanisms, then controlling the extrusion fixing strips to stretch by using the lower telescopic rods, clamping and fixing the bottom position of the culture bag by using the extrusion effect of the extrusion fixing strips of the two dual-mode multi-point culture bag clamping mechanisms, controlling the conical extrusion fixing rods to extend by using the upper telescopic rods, fixing the positions of two sides of the top of the culture bag by using the conical extrusion fixing rods of the two dual-mode multi-point culture bag clamping mechanisms, and simultaneously penetrating a liquid outlet hose of the culture bag into a through groove between the annular frame and the annular plate;
s3, the vertical mounting plate is controlled to rotate through the driving of the adjusting motor, so that the culture bags clamped in the dual-mode multi-point-position culture bag clamping mechanism are overturned, the cell liquid is discharged, the intercepting plate is driven to move by controlling the stretching of the micro telescopic rod in the discharging process, the liquid outlet hose of the culture bag is extruded by utilizing the extrusion effect of the intercepting plate to realize interception, and the intercepting plate is retracted when the liquid is required to be discharged;
s4, when the residual cell sap needs to be extruded, the transverse extrusion telescopic rod is used for controlling the extrusion plates to move so as to realize the extrusion effect, and the extrusion and the movement between the two extrusion plates are used for realizing the extrusion and the discharge of the residual cell sap in the cell culture bag.
(III) advantageous effects
Compared with the prior art, the invention provides a cell harvesting bracket for a cell culture bag and a using method thereof, and the cell harvesting bracket has the following beneficial effects:
the culture bag fixing device is simple to use, high in functionality, free of touch after the culture bag is fixed, high in intelligent degree and high in cleanliness.
Drawings
FIG. 1 is a schematic top view of the present invention;
FIG. 2 is a schematic front view of the present invention;
FIG. 3 is a schematic view of the backside structure of the present invention;
FIG. 4 is a schematic structural view of a dual-mode multi-site bag holding mechanism according to the present invention;
FIG. 5 is a schematic view of the catheter control mechanism of the present invention;
FIG. 6 is a schematic structural view of a vacuum adsorption module according to the present invention.
In the figure:
1. a chassis is detachably mounted;
2. a lateral baffle;
3. a suspension bracket;
4. a dual-mode multi-point position culture bag clamping mechanism; 4-1, adjusting a motor; 4-2, vertically installing the plate; 4-3, mounting a telescopic rod; 4-4, a lower telescopic rod; 4-5, carrying out conical extrusion on a fixed rod; 4-6, extruding the fixing strip; 4-7, longitudinally adjusting the telescopic rod; 4-8, transversely extruding the telescopic rod; 4-9, extruding a plate;
5. a catheter control mechanism; 5-1, a vacuum pump; 5-2, an air suction pipe; 5-3, adsorbing the disc;
6. a vacuum adsorption assembly; 6-1, Z-shaped stent; 6-2, a ring frame; 6-3, a micro telescopic rod; 6-4, a shutoff plate;
7. a weighing device.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Please refer to fig. 1-4.
Example one
A cell harvesting bracket for a cell culture bag comprises a detachable mounting base plate 1, a lateral baffle 2, a suspension bracket 3, a dual-mode multi-point position culture bag clamping mechanism 4, a vacuum adsorption component 6, a conduit control mechanism 5 and a weighing machine 7;
the side baffle 2 is welded on one side of the top of the detachable installation chassis 1, the suspension bracket 3 is welded on the top of the side baffle 2, and the two dual-mode multi-point culture bag clamping mechanisms 4 are respectively arranged at two ends of the top of the suspension bracket 3 and are used for clamping and fixing the cell culture bags and helping to harvest cells;
the vacuum adsorption component 6 is arranged on the detachable mounting base plate 1 in a penetrating way and is used for adsorbing a smooth desktop;
the conduit control mechanism 5 is fixedly arranged on the lateral baffle 2, and the conduit control mechanism 5 is positioned at the bottom of the dual-mode multi-point position culture bag clamping mechanism 4 and is used for clamping a liquid outlet hose of a culture bag and controlling liquid outlet through clamping and releasing;
a weight scale 7 is mounted on top of the removably mountable chassis 1 for weighing the container, the weight scale 7 being located at the bottom of the dual mode multi-position bag holding mechanism 4.
As a technical optimization scheme of the invention, as shown in FIG. 6, the vacuum adsorption component 6 comprises a vacuum pump 5-1, an air suction pipe 5-2 and an adsorption disc 5-3, the air suction pipe 5-2 is fixedly arranged in the detachable mounting base plate 1 in a penetrating manner, the vacuum pump 5-1 is arranged at the top of the detachable mounting base plate 1, the air suction end of the vacuum pump 5-1 is communicated with the air suction pipe 5-2, and the adsorption disc 5-3 is fixedly arranged at the bottom of the air suction pipe 5-2.
When the vacuum adsorption component 6 is actually used, the vacuum pump 5-1 sucks air into the air suction pipe 5-2, air in the adsorption disc 5-3 at the bottom is pumped out, the adsorption disc 5-3 is properly extruded after being in contact with a smooth desktop, the vacuum pump 5-1 is started again, so that air in the adsorption disc 5-3 is pumped out to form negative pressure, the support can be fixed on the desktop by utilizing the negative pressure, and the vacuum adsorption component is simple and convenient to operate, good in fixing effect and convenient to disassemble.
As a technical optimization scheme of the invention, as shown in FIG. 5, the catheter control mechanism 5 comprises a Z-shaped bracket 6-1, an annular frame 6-2, a micro telescopic rod 6-3 and a cut-off plate 6-4, wherein the annular frame 6-2 is welded at one end of the Z-shaped bracket 6-1 and is positioned at the bottom of the dual-mode multi-point culture bag clamping mechanism 4, the micro telescopic rod 6-3 is fixedly arranged at the inner edge side of the annular frame 6-2, the cut-off plate 6-4 is welded at the telescopic end of the micro telescopic rod 6-3, and a liquid outlet hose of a culture bag is arranged in a through groove between the annular frame 6-2 and the cut-off plate 6-4 in a penetrating manner.
When the conduit control mechanism 5 is actually used, the culture bag is clamped and fixed by the double-mode multi-point-position culture bag clamping mechanism 4, the liquid outlet hose of the culture bag is arranged in the through groove between the annular frame 6-2 and the intercepting plate 6-4 in a penetrating mode, the intercepting plate 6-4 is driven to move by controlling the stretching of the micro telescopic rod 6-3, the liquid outlet hose of the culture bag is extruded by utilizing the extrusion effect of the intercepting plate 6-4 to realize interception, and the intercepting plate 6-4 is retracted when liquid outlet is needed.
Example two
The structure of the dual-mode multi-point culture bag clamping mechanism 4 is customized on the basis of the first embodiment, as shown in fig. 4, the dual-mode multi-point culture bag clamping mechanism 4 comprises an adjusting motor 4-1, a vertical mounting plate 4-2, an upper telescopic rod 4-3, a lower telescopic rod 4-4, a tapered extrusion fixing rod 4-5, an extrusion fixing strip 4-6, a longitudinal adjusting telescopic rod 4-7, a transverse extrusion telescopic rod 4-8 and an extrusion plate 4-9, and the adjusting motor 4-1 is mounted on the suspension bracket 3 through a motor base, so that the adjusting motor 4-1 is in a fixed state and has a driving effect, and the adjusting motor is used for overturning the culture bag clamped in the dual-mode multi-point culture bag clamping mechanism 4 to discharge cell sap.
The central position of the vertical mounting plate 4-2 is connected with an output shaft of an adjusting motor 4-1, the upper telescopic rods 4-3 and the lower telescopic rods 4-4 are respectively mounted on one side of the top and one side of the bottom of the vertical mounting plate 4-2, the number of the upper telescopic rods 4-3 and the number of the lower telescopic rods 4-4 are two, the conical extrusion fixing rods 4-5 are fixed at the telescopic ends of the single upper telescopic rod 4-3, and the extrusion fixing strips 4-6 are welded between the telescopic ends of the two lower telescopic rods 4-4.
When the double-mode multi-point-position culture bag clamping mechanism 4 is in actual use, the lower telescopic rods 4-4 are used for controlling the extrusion fixing strips 4-6 to stretch, the bottom positions of the culture bags are clamped and fixed by utilizing the extrusion effect of the two extrusion fixing strips 4-6, the upper telescopic rods 4-3 are used for controlling the conical extrusion fixing rods 4-5 to stretch out, the two conical extrusion fixing rods 4-5 are used for fixing the positions of the two sides of the top of the culture bags, the two fixing modes are adopted, the extrusion fixing strips 4-6 are in a rectangular fixing range, a firmer fixing effect is realized, the extrusion fixing modes of the two conical extrusion fixing rods 4-5 are point-type fixing, and the liquid discharge of the culture bags after overturning is not influenced while the basic fixing effect is ensured.
As a technical optimization scheme of the invention, as shown in FIG. 4, a longitudinal adjusting telescopic rod 4-7 is fixedly arranged at one side of a vertical mounting plate 4-2, the telescopic end of the longitudinal adjusting telescopic rod 4-7 vertically faces to the middle point between two upper telescopic rods 4-3, a transverse extruding telescopic rod 4-8 is vertically fixed on the telescopic end of the longitudinal adjusting telescopic rod 4-7, an extrusion plate 4-9 is welded on the telescopic end of the transverse extruding telescopic rod 4-8, and one end of the extrusion plate 4-9 is in a round angle structure.
When the double-mode multi-point position culture bag clamping mechanism 4 is in actual use, the longitudinal adjusting telescopic rods 4-7 are used for controlling the transverse extrusion telescopic rods 4-8 and the extrusion plates 4-9 to move, the transverse extrusion telescopic rods 4-8 are used for controlling the extrusion plates 4-9 to move so as to realize the extrusion effect, and when in actual use, the extrusion and the movement between the two extrusion plates 4-9 are used for realizing the extrusion and the discharge of residual liquid in the cell culture bag, so that the functionality is improved.
A method of using a cell harvesting scaffold for a cell culture bag, comprising the steps of:
s1, wiping the desktop to be used, ensuring that excessive dust and impurities do not exist, then placing the position of the detachable mounting base plate 1 in a direction convenient to use, then pressing the detachable mounting base plate 1 and the vacuum adsorption component 6 downwards appropriately, starting the vacuum pump 5-1 while pressing to enable air in the adsorption plate 5-3 to be pumped out to form negative pressure, and fixing the detachable mounting base plate 1 on the desktop by utilizing the negative pressure;
s2, placing a culture bag to be subjected to cell harvesting between two dual-mode multi-point culture bag clamping mechanisms 4, then controlling the extrusion fixing strips 4-6 to stretch by using the lower telescopic rods 4-4, clamping and fixing the bottom position of the culture bag by using the extrusion effect of the extrusion fixing strips 4-6 of the two dual-mode multi-point culture bag clamping mechanisms 4, simultaneously controlling the conical extrusion fixing rods 4-5 to extend by using the upper telescopic rods 4-3, fixing the positions of two sides of the top of the culture bag by using the conical extrusion fixing rods 4-5 of the two dual-mode multi-point culture bag clamping mechanisms 4, and simultaneously penetrating the liquid outlet hose of the culture bag into the through groove between the annular frame 6-2 and the intercepting plate 6-4;
s3, the vertical mounting plate 4-2 is controlled to rotate by the driving of the adjusting motor 4-1, so that the culture bag clamped in the double-mode multi-point culture bag clamping mechanism 4 is overturned, the cell liquid is discharged, the intercepting plate 6-4 is driven to move by controlling the expansion and contraction of the micro telescopic rod 6-3 in the discharging process, the liquid outlet hose of the culture bag is extruded by the extrusion effect of the intercepting plate 6-4 to realize interception, and the intercepting plate 6-4 is retracted when the liquid needs to be discharged;
s4, when the residual cell sap needs to be extruded, the transverse extrusion telescopic rods 4-8 are used for controlling the extrusion plates 4-9 to move to realize the extrusion effect, and the extrusion and the movement between the two extrusion plates 4-9 are used for realizing the extrusion and the discharge of the residual liquid in the cell culture bag.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (10)
1. A cell harvesting support for cell culture bags is characterized in that: comprises a detachable mounting chassis (1), a lateral baffle (2), a suspension bracket (3), a dual-mode multi-point position culture bag clamping mechanism (4), a vacuum adsorption component (6), a conduit control mechanism (5) and a weighing machine (7);
the lateral baffle (2) is welded on one side of the top of the detachable mounting chassis (1), the suspension bracket (3) is welded on the top of the lateral baffle (2), and the two dual-mode multi-point culture bag clamping mechanisms (4) are respectively installed at two ends of the top of the suspension bracket (3);
the vacuum adsorption component (6) penetrates through the detachable installation base plate (1) and is used for adsorbing a smooth desktop;
the conduit control mechanism (5) is fixedly arranged on the lateral baffle (2), and the conduit control mechanism (5) is positioned at the bottom of the dual-mode multi-point position culture bag clamping mechanism (4);
the weighing device (7) is arranged at the top of the detachable installation chassis (1) and is used for weighing the container, and the weighing device (7) is positioned at the bottom of the dual-mode multi-point-position culture bag clamping mechanism (4).
2. A cell harvesting frame for a cell culture bag according to claim 1, wherein: the vacuum adsorption component (6) comprises a vacuum pump (5-1), an air suction pipe (5-2) and an adsorption disc (5-3), and the air suction pipe (5-2) is fixedly arranged in the detachable installation base plate (1) in a penetrating mode.
3. A cell harvesting frame for a cell culture bag according to claim 2, wherein: the vacuum pump (5-1) is installed at the top of the detachable installation base plate (1), the air suction end of the vacuum pump (5-1) is communicated with the air suction pipe (5-2), and the adsorption disc (5-3) is fixedly installed at the bottom of the air suction pipe (5-2).
4. A cell harvesting frame for a cell culture bag according to claim 1, wherein: the catheter control mechanism (5) comprises a Z-shaped bracket (6-1), an annular frame (6-2), a micro telescopic rod (6-3) and a cut-off plate (6-4), wherein the annular frame (6-2) is welded at one end of the Z-shaped bracket (6-1) and is positioned at the bottom of the dual-mode multi-point position culture bag clamping mechanism (4).
5. A cell harvesting frame for a cell culture bag according to claim 4, wherein: the miniature telescopic rod (6-3) is fixedly arranged on the inner side of the annular frame (6-2), the intercepting plate (6-4) is welded on the telescopic end of the miniature telescopic rod (6-3), and a liquid outlet hose of the culture bag is arranged in a through groove between the annular frame (6-2) and the intercepting plate (6-4) in a penetrating mode.
6. A cell harvesting frame for a cell culture bag according to claim 1, wherein: the double-mode multi-point-position culture bag clamping mechanism (4) comprises an adjusting motor (4-1), a vertical mounting plate (4-2), an upper telescopic rod (4-3), a lower telescopic rod (4-4), a conical extrusion fixing rod (4-5), an extrusion fixing strip (4-6), a longitudinal adjusting telescopic rod (4-7), a transverse extrusion telescopic rod (4-8) and an extrusion plate (4-9), wherein the adjusting motor (4-1) is installed on the suspension bracket (3) through a motor base.
7. A cell harvesting frame for a cell culture bag according to claim 6, wherein: the center position of the vertical mounting plate (4-2) is connected with an output shaft of the adjusting motor (4-1), and the upper telescopic rod (4-3) and the lower telescopic rod (4-4) are respectively mounted on one side of the top and one side of the bottom of the vertical mounting plate (4-2).
8. A cell harvesting frame for a cell culture bag according to claim 7, wherein: the number of the upper telescopic rods (4-3) and the number of the lower telescopic rods (4-4) are two, the conical extrusion fixing rods (4-5) are fixed at the telescopic ends of the upper telescopic rods (4-3), and the extrusion fixing strips (4-6) are welded between the telescopic ends of the two lower telescopic rods (4-4).
9. A cell harvesting frame for a cell culture bag according to claim 8, wherein: the longitudinal adjusting telescopic rods (4-7) are fixedly arranged on one side of the vertical mounting plate (4-2), the telescopic ends of the longitudinal adjusting telescopic rods (4-7) vertically face towards the middle point between the two upper telescopic rods (4-3), the transverse extruding telescopic rods (4-8) are vertically fixed on the telescopic ends of the longitudinal adjusting telescopic rods (4-7), the extruding plates (4-9) are welded on the telescopic ends of the transverse extruding telescopic rods (4-8), and one ends of the extruding plates (4-9) are of round angle structures.
10. The use of a cell harvesting frame for a cell culture bag according to any one of claims 1 to 9, comprising the steps of:
s1, wiping the tabletop to be used, ensuring that excessive dust and impurities do not exist, then placing the detachable mounting base plate (1) in a direction convenient to use, then pressing the detachable mounting base plate (1) and the vacuum adsorption component (6) downwards appropriately, starting the vacuum pump (5-1) while pressing to enable air in the adsorption plate (5-3) to be pumped out to form negative pressure, and fixing the detachable mounting base plate (1) on the tabletop by utilizing the negative pressure;
s2, placing the culture bag needing cell harvest between two dual-mode multi-point position culture bag clamping mechanisms (4), then the lower telescopic rod (4-4) is used for controlling the extrusion fixing strip (4-6) to stretch, the bottom position of the culture bag is clamped and fixed by the extrusion effect of the extrusion fixing strip (4-6) of the two double-mode multi-point position culture bag clamping mechanisms (4), meanwhile, the upper telescopic rod (4-3) controls the conical extrusion fixing rod (4-5) to extend out, the conical extrusion fixing rod (4-5) of the two double-mode multi-point-position culture bag clamping mechanisms (4) is utilized to fix the positions of two sides of the top of the culture bag, meanwhile, a liquid outlet hose of the culture bag is arranged in a through groove between the annular frame (6-2) and the shutoff plate (6-4) in a penetrating way;
s3, the vertical mounting plate (4-2) is controlled to rotate through the driving of the adjusting motor (4-1), so that the culture bags clamped in the dual-mode multi-point culture bag clamping mechanism (4) are overturned, the cell sap is discharged, the intercepting plate (6-4) is driven to move by controlling the stretching of the micro telescopic rod (6-3) in the discharging process, the liquid outlet hose of the culture bags is extruded to realize interception by utilizing the extrusion effect of the intercepting plate (6-4), and the intercepting plate (6-4) is retracted when the liquid needs to be discharged;
s4, when the residual cell sap needs to be extruded, the transverse extrusion telescopic rods (4-8) are used for controlling the extrusion plates (4-9) to move to realize the extrusion effect, and the extrusion and the movement between the two extrusion plates (4-9) are used for realizing the extrusion and the discharge of the residual cell sap in the cell culture bag.
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| CN202111653882.8A CN114308177B (en) | 2021-12-30 | 2021-12-30 | Cell harvesting bracket for cell culture bag and use method |
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| CN202111653882.8A CN114308177B (en) | 2021-12-30 | 2021-12-30 | Cell harvesting bracket for cell culture bag and use method |
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| CN114308177B CN114308177B (en) | 2023-02-10 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114653702A (en) * | 2022-05-18 | 2022-06-24 | 昕慕(江苏)生物医药科技有限公司 | Cell culture is intelligent cleaning device for accessory |
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