CN220724153U - Gravity column purification device - Google Patents
Gravity column purification device Download PDFInfo
- Publication number
- CN220724153U CN220724153U CN202321899727.9U CN202321899727U CN220724153U CN 220724153 U CN220724153 U CN 220724153U CN 202321899727 U CN202321899727 U CN 202321899727U CN 220724153 U CN220724153 U CN 220724153U
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- Prior art keywords
- tube
- fixedly connected
- filter membrane
- lower extreme
- purification device
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- 238000000746 purification Methods 0.000 title claims abstract description 35
- 230000005484 gravity Effects 0.000 title claims abstract description 20
- 239000012528 membrane Substances 0.000 claims abstract description 26
- 238000000926 separation method Methods 0.000 claims abstract description 21
- 230000006978 adaptation Effects 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 5
- 239000007788 liquid Substances 0.000 abstract description 15
- 239000002699 waste material Substances 0.000 abstract description 14
- 230000000670 limiting effect Effects 0.000 abstract description 7
- 239000012466 permeate Substances 0.000 abstract description 2
- 230000000694 effects Effects 0.000 description 4
- 238000001125 extrusion Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000000605 extraction Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000009194 climbing Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
Abstract
The utility model discloses a gravity column purification device which comprises a purification test tube, a fixing frame, a separation tube and a clamping ring, wherein the lower end of the separation tube is provided with a groove, the lower end of the separation tube is provided with a detachable filter membrane, the edge of the filter membrane is pressed and fixed through the clamping between the clamping ring and the groove, a hydraulic cylinder is fixedly arranged in the middle of the upper end of the fixing frame, the output end of the lower end of the hydraulic cylinder is fixedly connected with a pressing plate, the lower end of the pressing plate is fixedly connected with a fixed tube jacket at the left side of the middle position, the lower end of the pressing plate is fixedly connected with a limiting block at the middle position close to the right side, and the middle position of the limiting block penetrates through and is in sliding connection with a pin shaft. According to the utility model, the detachable filter membrane descends and extrudes to enable the waste liquid to permeate into the separating tube, and the waste liquid can be treated at one time after being lifted by controlling the separating tube, so that the efficiency of purifying cells is greatly improved, complicated transfer and manual operation are not required, and the risk of pollution to the cells is reduced.
Description
Technical Field
The utility model relates to the technical field of purification devices, in particular to a gravity column purification device.
Background
The basic idea of the gravity column purification principle is to separate the mixture into different layers by utilizing different sedimentation rates of different components in a gravity field, in practical operation, the mixture is usually added into a long and thin column, then the mixture is naturally settled in the column, and the different components are layered in the column due to different sedimentation rates, so that the separation is realized, in the cell culture process, a specific enzyme is required to eliminate the wall climbing phenomenon of cells and tissues in a culture dish, then the cells and the like in a culture medium are separated from waste liquid, so that new culture liquid is added, but in the waste liquid separation process, the operation is complicated, frequent manual operation of workers and back and forth transfer between instruments are required, the possibility of pollution of the cells is increased, and meanwhile, the separation and purification efficiency of the cells in the waste liquid is lower.
Disclosure of Invention
The utility model mainly aims to provide a gravity column purifying device which can effectively solve the problems in the background technology.
In order to achieve the above purpose, the technical scheme adopted by the utility model is as follows: the utility model provides a gravity post purification device, includes purification test tube, mount and separating tube, snap ring, the lower extreme of separating tube is provided with the recess, the lower extreme of separating tube is provided with detachable filter membrane, the edge of filter membrane is supported and is pressed fixedly through the block between snap ring and the recess, the department fixed mounting has the pneumatic cylinder in the middle of the upper end of mount, the lower extreme output fixedly connected with clamp plate of pneumatic cylinder, the lower extreme of clamp plate is in the left side department fixedly connected with of intermediate position and is decided the pipe clamp sleeve, the lower extreme of clamp plate is being close to the right side department fixedly connected with stopper in the middle of, the intermediate position of stopper runs through and sliding connection has the round pin axle, the left end fixedly connected with pipe clamp sleeve of round pin axle, the periphery of round pin axle is provided with the centre gripping spring between stopper and pipe clamp sleeve.
Preferably, the front end of mount is located down the position fixedly connected with and is placed the board, the centre department of placing the board is provided with the constant head tank, the outer wall of purification test tube is being close to upside department fixedly connected with solid fixed ring, looks adaptation between solid fixed ring and the constant head tank.
Preferably, the outer wall of the separation tube is attached to the inner wall of the purification test tube.
Preferably, the lower extreme of separator tube is close to outside department fixedly connected with three sets of L type fixture blocks that are circular array and arrange, the upper end of snap ring is close to outside department and is provided with three sets of draw-in grooves that are circular array and arrange, the upper end opening part of draw-in groove and the lower extreme looks adaptation of L type fixture block.
Preferably, the four corners of the pressing plate are penetrated with guide rods, and the guide rods are fixedly connected between the fixing frame and the placing plate.
Preferably, the pipe jacket and the fixed pipe clamp sleeve are symmetrical and are matched with the opening of the separating pipe.
Compared with the prior art, the utility model has the following beneficial effects:
through pasting the filter membrane that disinfects in the bottom of separator tube, then make the snap ring aim at the recess and push in, thereby the inside realization fixed of recess is advanced in the edge extrusion of filter membrane, thereby make the inside block of L type fixture block with the draw-in groove through rotating the snap ring, thereby accomplish the location between snap ring and the separator tube, avoid dropping and lead to the filter membrane to drop, easy operation is convenient when getting the filter membrane of trading simultaneously, it is fixed to separate the pipe centre gripping through fixing the pipe clamp cover and the pipe clamp cover, and then drive the lift through the pneumatic cylinder, in-process that descends because the extrusion effect, can make the waste liquid permeate into the inside of separator tube through the filter membrane, and the cell then can be blocked in the bottom of purification test tube, thereby purification separation operation between cell and the waste liquid has been accomplished, and the waste liquid that is separated can disposable after rising through controlling the separator tube, need not to carry out the extraction repeatedly, the efficiency of cell purification has been improved greatly, need not to carry out loaded down with trivial details transfer and manual operation simultaneously, the risk of cell contaminated has been reduced.
Drawings
FIG. 1 is a schematic diagram of the overall structure of a gravity column purification device according to the present utility model;
FIG. 2 is a schematic view of a gravity column purification device according to the present utility model in partial cross-section;
FIG. 3 is a schematic view of a gravity column purification device according to the present utility model in partial cross-section;
FIG. 4 is an enlarged schematic view of the gravity column purifying device of the present utility model at A in FIG. 1.
In the figure: 1. purifying the test tube; 2. a fixing frame; 3. placing a plate; 4. a positioning groove; 5. a fixing ring; 6. a separation tube; 7. a groove; 8. a filter membrane; 9. a clasp; 10. an L-shaped clamping block; 11. a clamping groove; 12. a hydraulic cylinder; 13. a pressing plate; 14. a guide rod; 15. a fixed pipe jacket; 16. a limiting block; 17. a pin shaft; 18. a tube jacket; 19. clamping springs.
Detailed Description
The utility model is further described in connection with the following detailed description, in order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the utility model easy to understand.
As shown in fig. 1-4, the gravity column purifying device comprises a purifying test tube 1, a fixing frame 2, a separating tube 6 and a clamping ring 9, wherein a groove 7 is formed in the lower end of the separating tube 6, a detachable filter membrane 8 is arranged at the lower end of the separating tube 6, the edge of the filter membrane 8 is pressed and fixed through the clamping between the clamping ring 9 and the groove 7, a hydraulic cylinder 12 is fixedly arranged in the middle of the upper end of the fixing frame 2, a pressing plate 13 is fixedly connected to the lower end output end of the hydraulic cylinder 12, a fixed tube clamp sleeve 15 is fixedly connected to the lower end of the pressing plate 13 at the left side of the middle position, a limiting block 16 is fixedly connected to the lower end of the pressing plate 13 at the middle position close to the right side, a pin 17 penetrates through and is slidingly connected to the middle of the limiting block 16, a tube clamp sleeve 18 is fixedly connected to the left end of the pin 17, and a clamping spring 19 is arranged between the limiting block 16 and the tube clamp sleeve 18;
the sterile filter membrane 8 is attached to the bottom end of the separation tube 6, then the clamping ring 9 is pressed into the groove 7, so that the edge of the filter membrane 8 is extruded into the groove 7 to realize fixation, further, the pin shaft 17 is pulled to drive the tube jacket 18 to move outwards, so that the separation tube 6 is clamped in the fixed tube jacket 15, the state is maintained, the pin shaft 17 is loosened, the tube jacket 18 is driven to reset under the rebound action of the clamping spring 19, the clamping fixation of the left end and the right end of the separation tube 6 is realized, the pressing plate 13 is driven by the starting hydraulic cylinder 12 to enable the separation tube 6 to be slowly inserted into the purification tube 1, waste liquid can penetrate into the separation tube 6 due to the extrusion effect in the descending process, cells can be blocked at the bottom of the purification tube 1, the purification separation operation between the cells and the waste liquid is finished, the separated waste liquid can be processed at one time after being lifted through the control of the separation tube 6, repeated extraction is not needed, the purification efficiency of the cells is greatly improved, the complicated transfer and the manual pollution operation is reduced.
The front end of mount 2 is put down position fixedly connected with and is placed board 3, and the centre department of placing board 3 is provided with constant head tank 4, and the outer wall of purification test tube 1 is being close to upside department fixedly connected with solid fixed ring 5, looks adaptation between gu fixed ring 5 and the constant head tank 4, is arranged in the fixed of each structure in this technical scheme through mount 2, supports and fixes purification test tube 1 through the block between gu fixed ring 5 and the constant head tank 4, avoids rocking.
The outer wall of the separation tube 6 is attached to the inner wall of the purification test tube 1, so that the situation that a gap exists to overflow cell tissues is avoided, and the feasibility of the technical scheme is ensured.
The lower extreme of separator tube 6 is being close to the L type fixture block 10 that three group was circular array and arranges in outside department fixedly connected with, the upper end of snap ring 9 is being close to outside department and is provided with three group's draw-in groove 11 that are circular array and arrange, the upper end opening part of draw-in groove 11 and the lower extreme looks adaptation of L type fixture block 10, before pressing snap ring 9, make L type fixture block 10 and the opening part of draw-in groove 11 align, thereby after pressing into recess 7, through the inside block of rotating snap ring 9 messenger L type fixture block 10 and draw-in groove 11, thereby accomplish the location between snap ring 9 and the separator tube 6, avoid dropping and lead to filter membrane 8 to drop, easy operation is convenient simultaneously when getting to trade filter membrane 8.
Guide rods 14 are penetrated at four corners of the pressing plate 13, the guide rods 14 are fixedly connected between the fixing frame 2 and the placing plate 3, stability of the pressing plate 13 during lifting can be guaranteed through limiting action of the guide rods 14, degree of fit between the separating tube 6 and the purifying test tube 1 is guaranteed, and the tube jacket 18 is symmetrical with the fixed tube jacket 15 and is matched with an opening of the separating tube 6, so that rationality among structures is guaranteed.
Working principle:
when in use, the cells in the culture medium, from which the wall climbing phenomenon is eliminated, are uniformly shaken and poured into the purification test tube 1, under the action of gravity, the cells with larger mass slowly settle to the bottom to realize the separation effect, but the process efficiency is slower, the sterilized filter membrane 8 is stuck to the bottom end of the separation tube 6, then the clamping ring 9 is pressed into the groove 7, the edge of the filter membrane 8 is extruded into the groove 7 to realize fixation, before the clamping ring 9 is pressed, the L-shaped clamping block 10 is aligned with the opening of the clamping groove 11, after the clamping ring 9 is pressed into the groove 7, the clamping ring 9 is rotated to clamp the L-shaped clamping block 10 with the inside of the clamping groove 11, thereby completing the positioning between the clamping ring 9 and the separation tube 6, avoiding the falling to lead the filter membrane 8 to fall off, meanwhile, the operation is simple and convenient when the filter membrane 8 is taken and replaced, the pin shaft 17 is pulled to drive the pipe jacket 18 to move outwards, the separating tube 6 is clamped in the fixed tube clamping sleeve 15, the state is maintained, the tube jacket 18 is driven to reset under the rebound action of the clamping spring 19 after the pin shaft 17 is loosened, the clamping and fixing of the left end and the right end of the separating tube 6 are realized, the pressure plate 13 is driven by the starting hydraulic cylinder 12, the separating tube 6 is slowly inserted into the purifying test tube 1, waste liquid can penetrate into the separating tube 6 due to the extrusion action in the descending process, cells can be blocked at the bottom of the purifying test tube 1 through the filter membrane 8, the purifying and separating operation between the cells and the waste liquid is completed, the separated waste liquid can be treated at one time after being lifted by controlling the separating tube 6 without repeated extraction, the purifying efficiency of the cells is greatly improved, and complicated transfer and manual operation are not required, the risk of contamination of the cells is reduced.
The foregoing has shown and described the basic principles and main features of the present utility model and the advantages of the present utility model. It will be understood by those skilled in the art that the present utility model is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present utility model, and various changes and modifications may be made without departing from the spirit and scope of the utility model, which is defined in the appended claims. The scope of the utility model is defined by the appended claims and equivalents thereof.
Claims (6)
1. The utility model provides a gravity post purification device, includes purification test tube (1), mount (2) and separator tube (6), snap ring (9), its characterized in that: the utility model discloses a filter membrane, including filter membrane, fixed frame, clamp ring (16), pneumatic cylinder (12) are fixed in the middle of the upper end of mount (2), lower extreme of separator tube (6) is provided with recess (7), the lower extreme of separator tube (6) is provided with detachable filter membrane (8), the edge of filter membrane (8) is supported through the block between snap ring (9) and recess (7) and is pressed fixedly, the lower extreme output fixedly connected with clamp plate (13) of pneumatic cylinder (12), the lower extreme of clamp plate (13) is fixed pipe clamp sleeve (15) in the left side department of intermediate position, the lower extreme of clamp plate (13) is fixed in the department of being close to right side centre fixedly connected with stopper (16), the intermediate position of stopper (16) runs through and sliding connection has round pin axle (17), the left end fixedly connected with of round pin axle (17) is equipped with clamping spring (19) between stopper (16) and pipe clamp sleeve (18).
2. A gravity column purification device according to claim 1, wherein: the front end of mount (2) is located down fixedly connected with and is placed board (3), the centre department of placing board (3) is provided with constant head tank (4), the outer wall of purification test tube (1) is being close to upside department fixedly connected with solid fixed ring (5), looks adaptation between solid fixed ring (5) and constant head tank (4).
3. A gravity column purification device according to claim 1, wherein: the outer wall of the separation tube (6) is attached to the inner wall of the purification test tube (1).
4. A gravity column purification device according to claim 1, wherein: the lower extreme of separator tube (6) is close to outside department fixedly connected with three sets of L type fixture blocks (10) that are circular array and arrange, the upper end of snap ring (9) is close to outside department and is provided with three sets of draw-in grooves (11) that are circular array and arrange, the upper end opening part of draw-in groove (11) and the lower extreme looks adaptation of L type fixture blocks (10).
5. A gravity column purification device according to claim 1, wherein: guide rods (14) penetrate through four corners of the pressing plate (13), and the guide rods (14) are fixedly connected between the fixing frame (2) and the placing plate (3).
6. A gravity column purification device according to claim 1, wherein: the pipe jacket (18) is symmetrical with the fixed pipe jacket (15) and is matched with the opening of the separating pipe (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321899727.9U CN220724153U (en) | 2023-07-18 | 2023-07-18 | Gravity column purification device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321899727.9U CN220724153U (en) | 2023-07-18 | 2023-07-18 | Gravity column purification device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220724153U true CN220724153U (en) | 2024-04-05 |
Family
ID=90500152
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321899727.9U Active CN220724153U (en) | 2023-07-18 | 2023-07-18 | Gravity column purification device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220724153U (en) |
-
2023
- 2023-07-18 CN CN202321899727.9U patent/CN220724153U/en active Active
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