CN207418769U - A kind of suspension cell culture device of sustainable dosing - Google Patents
A kind of suspension cell culture device of sustainable dosing Download PDFInfo
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- CN207418769U CN207418769U CN201721483638.0U CN201721483638U CN207418769U CN 207418769 U CN207418769 U CN 207418769U CN 201721483638 U CN201721483638 U CN 201721483638U CN 207418769 U CN207418769 U CN 207418769U
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- bottle
- dosing
- suspension cell
- doser
- cell culture
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The utility model discloses a kind of suspension cell culture devices of sustainable dosing, belong to biological technical field, it mainly includes blake bottle, doser and the culture plate of a uniform rotation, wherein, blake bottle includes integrally formed bottleneck and bottle, further include a bottle cap with bottleneck cooperation, bottle cap is equipped with a through hole, the chemical feed pipe of doser passes through through hole dosing into blake bottle, shape has angle between bottleneck and bottle bottom surface, the side wall in the in vivo culture solution outflow blake bottle of bottle and bottom surface is prevented to be equipped with a hydrophobic layer, the blake bottle and doser are placed in above the culture plate, and it is placed in parallel with culture plate.The utility model can effectively reduce the adherent probability of suspension cell, prevent that cell hypoxia is dead, the waste of suspension cell and suspension cell liquid is reduced simultaneously, improve the training quality of suspension cell, it solves the problems, such as the technical issues of waste of prior art suspension cell culture liquid, administration bad assurance, there is splendid practicability.
Description
Technical field
The utility model is related to biological technical fields, and in particular to a kind of suspension cell culture device of sustainable dosing.
Background technology
It is ground in any Physiology Experiment, pharmacological experiment, pharmacodynamic experiment, quality standard experiment, Drug toxicity trails etc.
In studying carefully, cell culture is selected to be very important one of experiment link.In fact, cell culture is a special kind of skill, it is also referred to as thin
Born of the same parents' clone technology (Ravi M, Paramesh V, Kaviya SR, et al.3D cell culture systems：
Advantages and applications [J] .J Cell Physiol, 2015,230 (1)：16-26.), cell culture
Main purpose is exactly the extensive clone of cell (one or more target cell) needed for realization, mainly including microbial cell
Culture clone, culture clone (Ng AH, Li BB, Chamberlain MD, the et al.Digital of human body cell
Microfluidic Cell Culture [J] .Annu Rev Biomed Eng, 2015, (17)：91-112.) etc..
In general, the culture clone of human body cell is paid much attention in the scientific research activity of our countries, such as can
To cultivate the normal cell of human body, the mutant cell (tumour cell) of human body can also be cultivated, it is known that for cell culture
For, suspension cell culture and attached cell culture are broadly divided into, wherein the former requires cell not adherent, and the latter requires cell
It does not suspend.Usually, suspended cell culture technic (Keller KC, Rodrigues B, Zur Nieden
NI.Suspension culture of pluripotent stem cells：effect of shear on stemcell
Fate [J] .Crit Rev Eukaryot Gene Expr, 2014,24 (1)：1-13.) it is a kind of extremely common cell culture
Technology, the technology requirement be subject to constantly vibrations, shake, agitation Liquid Culture environment in cultivate individual cells, multiple cells,
Small cell cluster etc. also requires suspension cell culture interface not exceed 5mm, if just needing constantly to stir culture solution more than 5mm,
With this increase culture suspension cell oxygen supply and carbon dioxide is promoted smoothly to discharge, obtained by suspension cell culture
Substantial amounts of cell or its metabolite, can carry out corresponding experimental study based on this.
But previous suspension cell culture inevitably causes suspension cell culture liquid apparent muddiness occur, so as to
The waste of suspension cell and suspension cell culture liquid is caused, while it is also possible to unnecessary dirt is brought to normal suspension cell
Dye, the in addition administration problem of suspension cell also bad assurance, this brings challenge for culture, the administration of suspension cell, it is seen that visits
The new cell culture technology of rope includes the culture of suspension cell, administration is just particularly important, normal in order to solve suspension cell
Rule culture, the basic problem of administration, the application devise a kind of suspension cell culture device of new sustainable dosing.
The content of the invention
To solve above-mentioned the deficiencies in the prior art, the utility model provides a kind of suspension cell culture of sustainable dosing
Device.
The technical solution of the utility model is as follows：
A kind of suspension cell culture device of sustainable dosing, includes the culture of blake bottle, doser and a uniform rotation
Tablet, wherein：
The blake bottle is torticollis blake bottle, including integrally formed bottleneck and bottle, further includes one and coordinates with bottleneck
Bottle cap, the bottle cap is equipped with a through hole, and the chemical feed pipe of the doser passes through through hole dosing into blake bottle；
Shape has angle between bottleneck and bottle bottom surface, prevents the side wall in the in vivo culture solution outflow blake bottle of bottle and bottom
Face is equipped with a hydrophobic layer；
The blake bottle and doser are placed in above the culture plate, and are placed in parallel with culture plate.
Preferably, the angle is 40 degree.
Preferably, thread fitting is passed through between the bottle cap and bottleneck.
Preferably, the doser is a piston injector, including injection head, dosing cylinder and piston, on the injection head
Connect a chemical feed pipe.
It is further preferred that the chemical feed pipe includes chemical feed pipe leading portion and chemical feed pipe back segment, chemical feed pipe leading portion and chemical feed pipe
Angle formed by back segment is 140 degree.
Preferably, set below the dosing cylinder there are two double end A-frame, be used to support the doser.
Preferably, the thickness of the hydrophobic layer is 0.02mm.
Preferably, the growth area of the blake bottle is 25cm2, the capacity of the doser is 5ml.
Preferably, the culture plate is fixedly connected with blake bottle and doser.
The utility model tool has the advantage that：
(1) blake bottle of the utility model and doser are placed on culture plate, the uniform rotation culture plate, energy
The suspension cell in blake bottle is enough promoted constantly to be vibrated in incubation, reduces the adherent probability of suspension cell.
(2) bottom of blake bottle, which is equipped with, thickeies hydrophobic region, can effectively prevent that suspension cell is adherent in torticollis blake bottle, into
And avoid suspension cell dead because of anoxic.
(3) the utility model can effectively reduce the waste of suspension cell, while can also reduce the wave of suspension cell liquid
Take, so as to ensure that the relative clean of normal suspension cell culture environment, improve the training quality of suspension cell.
Description of the drawings
Fig. 1 is the overall structure diagram of the utility model.
Reference sign：1- blake bottles；11- bottlenecks；12- bottles；13- bottle caps；2- dosers, 21- injection heads, 22-
Dosing cylinder, 221- precision scales；23- pistons；231- circular piston heads；232- circular piston bars；233- circular piston handles；24-
Chemical feed pipe；241- chemical feed pipe leading portions；242- chemical feed pipe back segments；3- culture plates；4- double end A-frames；5- suspension cell cultures
Liquid；51- culture solution liquid levels；6- hydrophobic layers.
Specific embodiment
In conjunction with implementation and attached drawing to the construction of the utility model, shape and the further details of explanation of feature.It should manage
Solution, the present embodiment are merely to illustrate the utility model rather than limit the scope of protection of the utility model.
It is the suspension cell culture device of the sustainable dosing of the utility model as shown in Figure 1, it is main to include growth area
For 25cm2Blake bottle 1, capacity be 5ml 2 and one uniform rotation of doser culture plate 3.Wherein, doser 2 is lived for one
Syringe is filled in, including injection head 21, dosing cylinder 22 and piston 23, a chemical feed pipe 24, the chemical feed pipe are connected on the injection head 21
24 include chemical feed pipe leading portion 241 and chemical feed pipe back segment 242, and angle formed by the chemical feed pipe leading portion 241 and chemical feed pipe back segment 242 is
140 degree, 22 lower section of dosing cylinder is set there are two double end A-frame 4, for preventing doser 2 from quivering in the dosing into blake bottle 1
It trembles.The piston 23 includes that sequentially connected circular piston is first 231,233 three parts of circular piston bar 232 and circular piston handle, and
Circular piston first 231 and circular piston bar 232 are filled in dosing cylinder 22.The dosing cylinder 22 is the agent structure of the doser 2,
Outer wall is equipped with accurate scale 221, for the accurately dosing into blake bottle 1.
Blake bottle 1 is torticollis blake bottle, includes integrally formed bottleneck 11 and bottle 12, one and 11 thread fitting of bottleneck
Bottle cap 13, bottle cap 13 is equipped with a through hole, the chemical feed pipe 24 of the doser 2 through through hole dosing into blake bottle 1, and
Chemical feed pipe leading portion 241 is in contact with the liquid levels in blake bottle 1.
One 40 degree of angles are formed between 12 bottom surface of bottleneck 11 and bottle, prevent the culture solution in bottle 12 from flowing out in blake bottle
Side wall and bottom surface be equipped with a thickness 0.02mm silicone hydroxyl hydrophobic layer 6.
Blake bottle 1 and doser 2 are placed in 3 top of culture plate, and are placed in parallel with culture plate 3, the culture plate 3
It is fixedly connected with blake bottle 1 and doser 2, prevents the device during uniform rotation of culture plate 3 from sliding.
The application method of the utility model is：Aseptically, doser 2 is made to draw drug, makes double end A-frame 4
Firm doser 2, and the chemical feed pipe leading portion 241 of doser 2 is contacted with the culture solution in blake bottle 1, at interval of for a period of time,
With regard to promoting circular piston handle 233, drug is promoted to pass through dosing cylinder 22, injection head 21, chemical feed pipe back segment 242, chemical feed pipe leading portion 241
And blake bottle 1 is reached in suspension cell culture liquid 5, completes dosing, and culture plate 3 is made to be rotated with 10-40 revs/min of speed,
So that culture solution whirling vibration, avoids cell attachment.
The description of the utility model is merely illustrative above, and not restrictive, those skilled in the art should be managed
Solution can carry out it within the spirit and scope of claim restriction many modifications, variation or equivalent modification, but they are all
It will fall into the scope of protection of the utility model.
Claims (9)
1. a kind of suspension cell culture device of sustainable dosing, which is characterized in that at the uniform velocity turn including blake bottle, doser and one
Dynamic culture plate, wherein：
The blake bottle is torticollis blake bottle, including integrally formed bottleneck and bottle, further includes a bottle with bottleneck cooperation
Lid, the bottle cap are equipped with a through hole, and the chemical feed pipe of the doser passes through through hole dosing into blake bottle；
Shape has angle between bottleneck and bottle bottom surface, prevents on side wall and the bottom surface in the in vivo culture solution outflow blake bottle of bottle
Equipped with a hydrophobic layer；
The blake bottle and doser are placed in above the culture plate, and are placed in parallel with culture plate.
2. the suspension cell culture device of sustainable dosing as described in claim 1, which is characterized in that the angle is 40
Degree.
3. the suspension cell culture device of sustainable dosing as described in claim 1, which is characterized in that the bottle cap and bottleneck
Between pass through thread fitting.
4. the suspension cell culture device of sustainable dosing as described in claim 1, which is characterized in that the doser is one
Including injection head, dosing cylinder and piston, a chemical feed pipe is connected on the injection head for piston injector.
5. the suspension cell culture device of sustainable dosing as described in claim 1, which is characterized in that the chemical feed pipe includes
Angle formed by chemical feed pipe leading portion and chemical feed pipe back segment, chemical feed pipe leading portion and chemical feed pipe back segment is 140 degree.
6. the suspension cell culture device of sustainable dosing as described in claim 1, which is characterized in that below the dosing cylinder
If there are two double end A-frames, the doser is used to support.
7. the suspension cell culture device of sustainable dosing as described in claim 1, which is characterized in that the thickness of the hydrophobic layer
It spends for 0.02mm.
8. the suspension cell culture device of sustainable dosing as described in claim 1, which is characterized in that the life of the blake bottle
Long area is 25cm2, the capacity of the doser is 5ml.
9. the suspension cell culture device of sustainable dosing as described in claim 1, which is characterized in that the culture plate with
Blake bottle and doser are fixedly connected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721483638.0U CN207418769U (en) | 2017-11-08 | 2017-11-08 | A kind of suspension cell culture device of sustainable dosing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721483638.0U CN207418769U (en) | 2017-11-08 | 2017-11-08 | A kind of suspension cell culture device of sustainable dosing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207418769U true CN207418769U (en) | 2018-05-29 |
Family
ID=62308272
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721483638.0U Expired - Fee Related CN207418769U (en) | 2017-11-08 | 2017-11-08 | A kind of suspension cell culture device of sustainable dosing |
Country Status (1)
| Country | Link |
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| CN (1) | CN207418769U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111548938A (en) * | 2020-06-09 | 2020-08-18 | 英诺维尔智能科技（苏州）有限公司 | Novel high-performance culture bottle applicable to suspension and adherent cell mixed culture |
-
2017
- 2017-11-08 CN CN201721483638.0U patent/CN207418769U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111548938A (en) * | 2020-06-09 | 2020-08-18 | 英诺维尔智能科技（苏州）有限公司 | Novel high-performance culture bottle applicable to suspension and adherent cell mixed culture |
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| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180529 Termination date: 20181108 |