CN112410217A - Centrifugal culture bottle - Google Patents
Centrifugal culture bottle Download PDFInfo
- Publication number
- CN112410217A CN112410217A CN202011226746.6A CN202011226746A CN112410217A CN 112410217 A CN112410217 A CN 112410217A CN 202011226746 A CN202011226746 A CN 202011226746A CN 112410217 A CN112410217 A CN 112410217A
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- China
- Prior art keywords
- bottle
- lid
- shell
- breathable film
- ventilated membrane
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- 239000012528 membrane Substances 0.000 claims description 24
- 238000007789 sealing Methods 0.000 claims description 17
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
- 239000000741 silica gel Substances 0.000 claims description 5
- 229910002027 silica gel Inorganic materials 0.000 claims description 5
- 241000463219 Epitheca Species 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 20
- 230000008569 process Effects 0.000 abstract description 20
- 238000004519 manufacturing process Methods 0.000 abstract description 13
- 238000004113 cell culture Methods 0.000 abstract description 3
- 238000005119 centrifugation Methods 0.000 description 11
- 239000000463 material Substances 0.000 description 4
- 230000035699 permeability Effects 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 238000010327 methods by industry Methods 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000012007 large scale cell culture Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/24—Gas permeable parts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/10—Separation or concentration of fermentation products
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to the technical field of centrifugal culture bottles for cell culture, in particular to a centrifugal culture bottle which comprises a bottle cap, a bottle cap rubber gasket, a bottle body, a breathable film and a breathable film cap, wherein an operation interface is arranged on the end face of the bottle cap, the bottle cap is arranged on the bottle opening of the bottle body, the bottle cap rubber gasket is arranged at the joint of the bottle cap and the bottle opening of the bottle body, and the bottle body comprises an upper bottle body shell, a lower bottle body shell and a conical bottom. Simple process flow, low cost, time saving and production efficiency improvement.
Description
Technical Field
The invention relates to the technical field of centrifugal culture bottles for cell culture, in particular to a centrifugal culture bottle.
Background
The biological medicine is in a rapid development stage, in the biological pharmacy process, large-scale cell culture is complex work, and a proper cell culture process is adopted, so that the key for efficiently and successfully obtaining the culture process is realized.
The culture bottle in the current market can only be used for culture and can not be used for centrifugation, so that the process needs to be frequently switched between the culture bottle and the centrifugation bottle in the whole process engineering, a large amount of time, manpower, material resources and financial resources are wasted, the operation is complex and complicated, the problem of unpredictable property is easy to occur, the production efficiency is not high enough, and therefore a compound centrifugation culture bottle which can be used as the culture bottle and can be used for centrifugation is urgently needed to be pushed to the market, the process flow is simplified, the cost is reduced, the time is saved, and the production efficiency is improved.
Disclosure of Invention
The present invention is directed to a centrifugal culture flask, which solves the above problems of the prior art.
In order to achieve the purpose, the invention provides the following technical scheme:
the utility model provides a centrifugal culture bottle, includes bottle lid, bottle lid cushion, body, ventilated membrane and ventilated membrane lid, the operation interface has been seted up on the terminal surface of bottle lid, the bottle lid is installed on the bottleneck of body, the bottle lid cushion sets up the junction at the bottleneck of bottle lid and body, the body includes body epitheca, body inferior valve and awl end, inside the ventilated membrane mounting groove of ventilated membrane setting seted up on the lateral wall of body inferior valve, the inside of ventilated membrane mounting groove is and the outside correspondence that is located the ventilated membrane is provided with ventilated membrane lid, the ventilated membrane covers and has evenly seted up the bleeder vent.
Preferably, the bottle cap is in threaded connection with the bottle opening of the bottle body, and the bottle cap rubber gasket is an integrally formed silica gel sealing gasket.
Preferably, the upper bottle body shell is arranged at the end part of the lower bottle body shell, the conical bottom is arranged at the bottom of the lower bottle body shell and is of a conical structure, and the upper bottle body shell, the lower bottle body shell and the conical bottom are integrally formed.
Preferably, the breathable film mounting groove, the breathable film and the breathable film cover are symmetrically arranged on the opposite side walls of the lower bottle body shell based on the bottle cap as an axis.
Preferably, the breathable film cover, the bottle body and the edge part of the breathable film form a sealing system.
Preferably, the operation interface is a special interface and is suitable for a machine automation switch cover.
Compared with the prior art, the invention has the beneficial effects that:
1. in the invention, the requirements can be met simultaneously in the process of centrifugation and culture processes through design; the structure is simple, the sealing is facilitated, the cost is low, the realization is easy, one bottle is multipurpose, and the culture cost is reduced; the secondary pollution caused by the process switching between the centrifugal bottle and the culture bottle is avoided, the sterile level of the whole culture and centrifugation process is improved, and the culture success rate is improved; the bottom of the bottle body is designed in a conical shape, so that the sedimentation and collection of the centrifugal extract are facilitated; the bottle cap is provided with an interface suitable for automatically opening and closing the cap by a machine, so that the bottle cap can be used for large-batch automatic production and small-batch manual production, and one bottle has multiple purposes; during artificial culture, the operation process of manual reciprocation between the centrifugal bottle and the culture bottle is reduced, the labor cost and the error rate of manual operation are reduced, the process flow is simplified, and the production efficiency and the culture success rate are improved; when the equipment is produced in batches, the action flow and the error rate of the equipment are reduced, the functional requirements on the equipment are reduced, the cost is reduced, the process flow is simplified, and the production efficiency and the successful culture are improved; therefore, the problem that the culture bottle in the current market can only be used for culture and can not be used for centrifugation, so that the process needs to be frequently switched between the culture bottle and the centrifugation bottle in the whole process engineering, a large amount of time, manpower, material resources, financial resources and complex and complicated operation are wasted, and unpredictable problems are easy to occur is solved.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of a portion of the present invention;
fig. 3 is an overall sectional structural view of the present invention.
In the figure: 1-bottle cap, 2-bottle cap rubber pad, 3-bottle body, 4-breathable film, 5-breathable film cap, 6-breathable film mounting groove, 7-breathable hole, 101-operation interface, 301-bottle body upper shell, 302-bottle body lower shell and 303-conical bottom.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.
Referring to fig. 1-3, the present invention provides a technical solution:
the utility model provides a centrifugal culture bottle, including bottle lid 1, bottle lid cushion 2, body 3, ventilated membrane 4 and ventilated membrane lid 5, operation interface 101 has been seted up on the terminal surface of bottle lid 1, bottle lid 1 is installed on the bottleneck of body 3, bottle lid cushion 2 sets up the junction at the bottleneck of bottle lid 1 with body 3, body 3 includes body epitheca 301, body inferior valve 302 and awl end 303, ventilated membrane 4 sets up inside ventilated membrane mounting groove 6 of seting up on the lateral wall of body inferior valve 302, ventilated membrane mounting groove 6's inside is and the outside correspondence that is located ventilated membrane 4 is provided with ventilated membrane lid 5, breathable membrane lid 5 is last evenly to have seted up bleeder vent 7.
Furthermore, the bottle cap 1 is connected with the bottle opening of the bottle body 3 in a threaded manner, and the effect of the threaded connection is that when the bottle cap 1 is screwed, the bottle cap 1 is attached to the inner protrusion of the bottle opening of the bottle body 3, and reliable sealing is ensured under the squeezing of the cap rubber gasket 2; the bottle cap rubber gasket 2 is an integrally formed silica gel sealing gasket and has the advantages that the silica gel material has good biocompatibility and can reliably ensure sealing. A positioning structure is provided between the rubber pad and the bottle cap, so that the positioning and the installation are convenient.
Further, the upper bottle body shell 301 is arranged at the end of the lower bottle body shell 302, the conical bottom 303 is arranged at the bottom of the lower bottle body shell 302 and is of a conical structure, the upper bottle body shell 301, the lower bottle body shell 302 and the conical bottom 303 are integrally formed, the upper bottle body shell 301 and the lower bottle body shell 302 form a culture carrier, the conical bottom 303 is arranged at the bottom of the bottle body, so that the centrifuged extract can be conveniently settled to the tip of the conical bottom 303, and the centrifuged extract can be conveniently collected;
further, the air-permeable film mounting groove 6, the air-permeable film 4 and the air-permeable film cover 5 are symmetrically arranged on the opposite side walls of the lower bottle body shell 302 based on the bottle cap 1 as an axis, wherein the air-permeable film 4 has the functions of air permeability and water impermeability, so that the cultured object cannot leak, air exchange can be performed, the cultured object can obtain enough air permeability, and the air exchange requirement required by the culture process is met;
further, the breathable film cover 5, the bottle body 3 and the edge part of the breathable film 4 form a sealing system, so that reliable sealing is ensured, and the breathable film 4 is fixed;
furthermore, the operation interface 101 is a special interface, is suitable for automatic opening and closing of a machine cover, and has the functions of being used for large-batch automatic production and small-batch manual production, and one bottle has multiple purposes.
The specific implementation case is as follows:
as shown in fig. 1 to 3, the centrifugal culture flask mainly comprises:
1) the bottle cap 1 and the bottle body 3 are sealed by threads, when the bottle cap is screwed, the bottle opening is attached to the inner protrusion of the bottle cap, and reliable sealing is guaranteed under the squeezing of the sealing rubber gasket. The bottle cap 1 is provided with an operation interface 101, and the operation interface 101 is suitable for opening and closing the cap by an automatic machine, so that automatic batch production is facilitated;
2) the bottle cap rubber gasket 2 is arranged between the bottle cap 1 and the bottle body 3, and the integrally formed silica gel sealing gasket is made of a material with good biocompatibility and reliably ensures sealing. A positioning structure is provided between the rubber pad and the bottle cap, so that the positioning and the installation are convenient;
3) the bottle body 3 comprises a bottle body upper shell 301 and a bottle body lower shell 302, which form a culture carrier, the bottom of the bottle body is a conical bottom 303, so that the extract is conveniently precipitated to the tip of the conical bottom 303 after centrifugation, and the centrifugal extract is conveniently collected;
4) the air-permeable film 4 is arranged on the side surface of the bottle body 3, the air-permeable film 4 can be permeable and impermeable to air, so that the cultured substance cannot leak, and air exchange can be carried out, so that the cultured substance can obtain enough air permeability, and the air exchange requirement required by the culture process is met;
5) a certain amount of air holes 7 are arranged on the breathable film cover 5 to provide air exchange in the culture process, and form a sealing system with the bottle body 3 and the edge part of the breathable film 4 to ensure reliable sealing and fix the breathable film 4.
Therefore, the invention achieves the following game effects through design:
1. the centrifugation and culture process can simultaneously meet the requirements;
2. the structure is simple, the sealing is facilitated, the cost is low, the realization is easy, one bottle is multipurpose, and the culture cost is reduced;
3. the secondary pollution caused by the process switching between the centrifugal bottle and the culture bottle is avoided, the sterile level of the whole culture and centrifugation process is improved, and the culture success rate is improved;
4. the bottom of the bottle body is designed in a conical shape, so that the sedimentation and collection of the centrifugal extract are facilitated;
5. the bottle cap is provided with an interface suitable for automatically opening and closing the cap by a machine, so that the bottle cap can be used for large-batch automatic production and small-batch manual production, and one bottle has multiple purposes;
6. during artificial culture, the operation process of manual reciprocation between the centrifugal bottle and the culture bottle is reduced, the labor cost and the error rate of manual operation are reduced, the process flow is simplified, and the production efficiency and the culture success rate are improved;
7. when the equipment is produced in batches, the action flow and the error rate of the equipment are reduced, the functional requirements on the equipment are reduced, the cost is reduced, the process flow is simplified, and the production efficiency and the successful culture are improved;
although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. The utility model provides a centrifugal culture bottle, includes bottle lid (1), bottle lid cushion (2), body (3), ventilated membrane (4) and ventilated membrane lid (5), its characterized in that: the operation interface (101) has been seted up on the terminal surface of bottle lid (1), install on the bottleneck of body (3) bottle lid (1), bottle lid cushion (2) set up the junction at the bottleneck of bottle lid (1) and body (3), body (3) are including body epitheca (301), body inferior valve (302) and awl end (303), ventilated membrane (4) set up inside ventilated membrane mounting groove (6) of seting up on the lateral wall of body inferior valve (302), the inside of ventilated membrane mounting groove (6) is and the outside correspondence that is located ventilated membrane (4) is provided with ventilative membrane lid (5), bleeder vent (7) have evenly been seted up on ventilative membrane lid (5).
2. A centrifugal culture flask according to claim 1, characterized in that: the bottle cap (1) is in threaded connection with the bottle opening of the bottle body (3), and the bottle cap rubber gasket (2) is an integrally formed silica gel sealing gasket.
3. A centrifugal culture flask according to claim 1, characterized in that: the upper bottle shell (301) is arranged at the end of the lower bottle shell (302), the conical bottom (303) is arranged at the bottom of the lower bottle shell (302) and is of a conical structure, and the upper bottle shell (301), the lower bottle shell (302) and the conical bottom (303) are integrally formed.
4. A centrifugal culture flask according to claim 1, characterized in that: the breathable film mounting groove (6), the breathable film (4) and the breathable film cover (5) are symmetrically arranged on the opposite side walls of the lower bottle body shell (302) based on the bottle cap (1) as an axis.
5. A centrifugal culture flask according to claim 1, characterized in that: the breathable film cover (5), the bottle body (3) and the edge part of the breathable film (4) form a sealing system.
6. A centrifugal culture flask according to claim 1, characterized in that: the operating interface (101) is a purpose-built interface and is suitable for machine automation switch covers.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011226746.6A CN112410217A (en) | 2020-11-06 | 2020-11-06 | Centrifugal culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011226746.6A CN112410217A (en) | 2020-11-06 | 2020-11-06 | Centrifugal culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112410217A true CN112410217A (en) | 2021-02-26 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011226746.6A Pending CN112410217A (en) | 2020-11-06 | 2020-11-06 | Centrifugal culture bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112410217A (en) |
Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996030274A1 (en) * | 1995-03-31 | 1996-10-03 | Smithkline Beecham Corporation | Tube closure |
| US20090148941A1 (en) * | 2007-07-30 | 2009-06-11 | Peter Florez | Disposable mini-bioreactor device and method |
| CN205741021U (en) * | 2016-05-10 | 2016-11-30 | 广州市达晖生物技术有限公司 | A kind of centrifugal tubular type sterile culture flask |
| CN206204332U (en) * | 2016-11-16 | 2017-05-31 | 杭州师范大学 | Cell is centrifuged blake bottle |
| CN206385176U (en) * | 2017-01-12 | 2017-08-08 | 湖南丰晖生物科技有限公司 | Suspending cell culture bottle |
| CN206418144U (en) * | 2017-01-13 | 2017-08-18 | 北京华诺奥美医学检验所有限公司 | A kind of permeability type lymphocyte blake bottle |
| CN109401972A (en) * | 2018-12-07 | 2019-03-01 | 英诺维尔智能科技（苏州）有限公司 | A kind of attached cell culture vessel suitable for automation equipment |
| CN109706077A (en) * | 2019-01-21 | 2019-05-03 | 英诺维尔智能科技（苏州）有限公司 | A kind of Novel breathable culture bottle |
| CN109762740A (en) * | 2019-02-22 | 2019-05-17 | 英诺维尔智能科技（苏州）有限公司 | A kind of novel hand-held bilateral vapor-permeable type culture bottle |
| CN109852547A (en) * | 2019-03-14 | 2019-06-07 | 英诺维尔智能科技（苏州）有限公司 | A kind of group variety layout of full-automation biological products system |
| CN110628616A (en) * | 2019-09-20 | 2019-12-31 | 江南大学 | Reactor system and application thereof |
| US20200123486A1 (en) * | 2018-10-19 | 2020-04-23 | SCL Biotech Ltd. | Culture flask and culture flask assembly |
| CN111548938A (en) * | 2020-06-09 | 2020-08-18 | 英诺维尔智能科技（苏州）有限公司 | Novel high-performance culture bottle applicable to suspension and adherent cell mixed culture |
| CN211522193U (en) * | 2019-12-17 | 2020-09-18 | 河南科技学院 | Ventilation type is cultivateed and is used centrifuging tube |
-
2020
- 2020-11-06 CN CN202011226746.6A patent/CN112410217A/en active Pending
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996030274A1 (en) * | 1995-03-31 | 1996-10-03 | Smithkline Beecham Corporation | Tube closure |
| US20090148941A1 (en) * | 2007-07-30 | 2009-06-11 | Peter Florez | Disposable mini-bioreactor device and method |
| CN205741021U (en) * | 2016-05-10 | 2016-11-30 | 广州市达晖生物技术有限公司 | A kind of centrifugal tubular type sterile culture flask |
| CN206204332U (en) * | 2016-11-16 | 2017-05-31 | 杭州师范大学 | Cell is centrifuged blake bottle |
| CN206385176U (en) * | 2017-01-12 | 2017-08-08 | 湖南丰晖生物科技有限公司 | Suspending cell culture bottle |
| CN206418144U (en) * | 2017-01-13 | 2017-08-18 | 北京华诺奥美医学检验所有限公司 | A kind of permeability type lymphocyte blake bottle |
| US20200123486A1 (en) * | 2018-10-19 | 2020-04-23 | SCL Biotech Ltd. | Culture flask and culture flask assembly |
| CN109401972A (en) * | 2018-12-07 | 2019-03-01 | 英诺维尔智能科技（苏州）有限公司 | A kind of attached cell culture vessel suitable for automation equipment |
| CN109706077A (en) * | 2019-01-21 | 2019-05-03 | 英诺维尔智能科技（苏州）有限公司 | A kind of Novel breathable culture bottle |
| CN109762740A (en) * | 2019-02-22 | 2019-05-17 | 英诺维尔智能科技（苏州）有限公司 | A kind of novel hand-held bilateral vapor-permeable type culture bottle |
| CN109852547A (en) * | 2019-03-14 | 2019-06-07 | 英诺维尔智能科技（苏州）有限公司 | A kind of group variety layout of full-automation biological products system |
| CN110628616A (en) * | 2019-09-20 | 2019-12-31 | 江南大学 | Reactor system and application thereof |
| CN211522193U (en) * | 2019-12-17 | 2020-09-18 | 河南科技学院 | Ventilation type is cultivateed and is used centrifuging tube |
| CN111548938A (en) * | 2020-06-09 | 2020-08-18 | 英诺维尔智能科技（苏州）有限公司 | Novel high-performance culture bottle applicable to suspension and adherent cell mixed culture |
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Application publication date: 20210226 |