CN212476773U

CN212476773U - Automatic change cell culture device of liquid

Info

Publication number: CN212476773U
Application number: CN202020665687.1U
Authority: CN
Inventors: 李璐佳; 刘�东
Original assignee: Huaxiayuan Cell Engineering Group Co Ltd
Current assignee: Huaxiayuan (Shanghai) Biotechnology Co.,Ltd.
Priority date: 2020-04-27
Filing date: 2020-04-27
Publication date: 2021-02-05
Anticipated expiration: 2030-04-27

Abstract

The utility model discloses a cell culture device capable of automatically changing liquid, which belongs to the technical field of cell culture, and comprises a liquid storage dish, an outer culture dish and a waste liquid dish which are sequentially embedded from top to bottom, the outer culture dish is embedded and connected with the inner culture dish, a plurality of first drainage tubes are fixedly inserted downwards along the axis direction of the inner bottom of the liquid storage dish, the top end parts of the first drainage tubes are provided with first valves, old culture liquid is drained into the waste liquid dish through the drainage of an upper capillary mode and a lower capillary mode, new culture liquid is led into the inner culture dish, the device is simple and convenient, special liquid changing equipment is not needed, only a sufficient culture medium is added once when cells are inoculated, the subsequent days do not need manual liquid changing, the workload is reduced to a certain extent, the manpower is liberated, and meanwhile, the cells are not influenced through a soft drainage mode, reduces the interference of human operation factors on cells during cell culture.

Description

Automatic change cell culture device of liquid

Technical Field

The utility model relates to a cell culture technical field, more specifically say, relate to an automatic cell culture device who trades liquid.

Background

In the field of cell biology research and the field of life science research, cell culture is the most basic procedure, and culture solution and cells are added to a culture dish, the culture dish is sealed, and the culture dish is stored in a culture chamber in which the environment such as temperature and gas atmosphere is controlled to perform culture.

In the process of cell culture, the timed and quantitative replacement of cell culture solution is the most critical operation. At present, the cell culture liquid change mode that laboratory was commonly used is realized through artifical manual operation, each regularly takes out the culture dish from the culture room, move and annotate the operating space that divides into, the separation, change the culture solution here, send back again in the culture room, repeatedly carry out such operation with the cultured cell, to the longer cell that just needs the change liquid every day of culture cycle, it is comparatively loaded down with trivial details to change liquid every day, and consume great manpower, it is very inconvenient, in addition, because the operating dynamics is great when artifical manual change culture solution, easily cause the influence to the cultivation of cell when taking out old culture solution or leading-in new culture solution.

Therefore, we propose a cell culture device with automatic liquid change to effectively solve some problems existing in the prior art.

SUMMERY OF THE UTILITY MODEL

1. Technical problem to be solved

To the problem that exists among the prior art, the utility model aims to provide an automatic cell culture device who trades liquid, disposable add sufficient culture medium when only needing the inoculation cell, follow-up several days do not all need the manual work to trade liquid, have reduced work load to a certain extent, have liberated the manpower, and simultaneously, through gentle drainage mode, can not cause the influence to the cell, have reduced the interference of manual operation factor to the cell during cell culture.

2. Technical scheme

In order to solve the above problems, the utility model adopts the following technical proposal.

A cell culture device capable of automatically changing liquid comprises a liquid storage dish, an outer culture dish and a waste liquid dish which are sequentially embedded from top to bottom, wherein the outer culture dish is embedded with and connected with the inner culture dish, the inner bottom of the liquid storage dish is fixedly downwards inserted and provided with a plurality of first drainage tubes along the axis direction, the top end parts of the first drainage tubes are provided with a first valve, the first drainage tubes are sleeved with a first water absorption wool rope and downwards extend into the inner culture dish, the side wall of the inner culture dish is downwards inserted and provided with a plurality of second drainage tubes along the axis direction, the top end parts of the second drainage tubes are provided with a second valve, the second drainage tubes are sleeved with a second water absorption wool rope, the second water absorption wool ropes downwards extend into the waste liquid dish, one side wall of the waste liquid dish is provided with a drain pipe, and accelerating drainage mechanisms corresponding to the second water absorption wool are fixedly arranged on the peripheral inner walls of the waste liquid vessel, and the bottom ends of the second water absorption wool are connected with the accelerating drainage mechanisms.

Further, the bottom of liquid storage ware is equipped with and establishes sealed chamber with inlaying of outer culture dish top end seal linking, the fixed cover in bottom portion of outer culture dish is equipped with the sealed lid of annular that links up with waste liquid dish top end seal, and is a plurality of first drainage tube runs through to inlaying the bottom of establishing sealed chamber and extends downwards, and outer culture dish is open state with the top portion of waste liquid dish, and when carrying out cell culture, liquid storage ware, outer culture dish, waste liquid dish inlay layer upon layer and establish sealed linking, have sealed lid on the liquid storage dish certainly, effectively realize cell culture's pollution-free nature.

Further, the top lateral wall of outer culture dish and waste liquid ware goes up the cladding and has the sealing washer, inlay the inside wall of establishing sealed chamber and annular seal lid and respectively with two sealing washer seal joint that distribute from top to bottom.

Furthermore, an annular cavity used for installing a second drainage tube is reserved between the inner culture dish and the outer culture dish, and the top end part of the second drainage tube is of a bent pipe structure.

Further, drainage mechanism includes the honeycomb duct through mount pad fixed connection on waste liquid ware inner wall with higher speed, the powerful sponge that absorbs water of fixedly connected with on one side inner wall of the mounting pad of keeping away from of honeycomb duct, the inside fixed mounting of mount pad has electric telescopic handle, electric telescopic handle's flexible end runs through honeycomb duct and fixedly connected with and the stripper plate that the sponge position corresponds of absorbing water strongly, and at the in-process of drainage, the stripper plate can constantly extrude the powerful sponge that absorbs water, extrudees away the culture solution in the powerful sponge that absorbs water, effectively improves the adsorption efficiency of the powerful sponge that absorbs water to accelerate the change of old culture solution.

Furthermore, the bottom end of the second water absorption wool is connected with the top end of the strong water absorption sponge.

3. Advantageous effects

Compared with the prior art, the utility model has the advantages of:

(1) this scheme is through the drainage of upper and lower capillary mode, in going into waste liquid dish with old culture solution drainage, in the culture dish in introducing new culture solution, the device is simple and convenient, need not special liquid changing equipment, disposable sufficient culture medium that adds when only needing the inoculation cell, follow-up several days do not all need the manual work to change the liquid, the work load has been reduced to a certain extent, the manpower has been liberated, and simultaneously, through gentle drainage mode, can not cause the influence to the cell, the interference of manual operation factor to the cell during the cell culture has been reduced.

(2) Drainage mechanism includes through honeycomb duct of mount pad fixed connection on waste liquid ware inner wall with higher speed, the powerful sponge that absorbs water of fixedly connected with on one side inner wall of keeping away from the mount pad of honeycomb duct, the inside fixed mounting of mount pad has electric telescopic handle, electric telescopic handle's flexible end runs through honeycomb duct and fixedly connected with and the stripper plate that the sponge position corresponds of absorbing water with great force, at the in-process of drainage, the stripper plate can constantly extrude the sponge that absorbs water with great force, extrude away the culture solution in the sponge that absorbs water with great force, effectively improve the adsorption efficiency of sponge that absorbs water, thereby accelerate the change of old culture solution.

Drawings

Fig. 1 is a perspective view of the present invention;

FIG. 2 is an exploded view of the present invention;

FIG. 3 is a bottom view of the liquid outlet vessel of the present invention;

FIG. 4 is a bottom view of the external culture dish of the present invention;

fig. 5 is a perspective view of the accelerated drainage mechanism of the present invention.

The reference numbers in the figures illustrate:

the device comprises a liquid storage dish 1, a liquid storage dish 101, an embedded sealing cavity 2, an external culture dish 2, a waste liquid dish 3, a first drainage tube 4, a first water absorption wool 5, a first valve 6, an internal culture dish 7, a second drainage tube 8, a second water absorption wool 9, a second valve 10, an annular sealing cover 11, an accelerating drainage mechanism 12, a flow guide tube 121, a strong water absorption sponge 122, a squeezing plate 123, a mounting seat 124 and a sewage discharge pipe 13.

Detailed Description

The drawings in the embodiments of the present invention will be combined; the technical scheme in the embodiment of the utility model is clearly and completely described; obviously; the described embodiments are only some of the embodiments of the present invention; but not all embodiments, are based on the embodiments of the invention; all other embodiments obtained by a person skilled in the art without making any inventive step; all belong to the protection scope of the utility model.

In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "top/bottom", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted", "provided", "sleeved/connected", "connected", and the like are to be understood in a broad sense, such as "connected", which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Example 1:

referring to fig. 1-4, an automatic liquid changing cell culture device comprises a liquid storage dish 1, an outer culture dish 2 and a waste liquid dish 3 which are sequentially embedded and connected from top to bottom, the outer culture dish 2 is embedded and connected with an inner culture dish 7, cells are cultured in the inner culture dish 7, an embedded sealing cavity 101 which is hermetically connected with the top end of the outer culture dish 2 is arranged at the bottom end of the liquid storage dish 1, an annular sealing cover 11 which is hermetically connected with the top end of the waste liquid dish 3 is fixedly sleeved at the bottom end of the outer culture dish 2, a plurality of first drainage tubes 4 penetrate through the bottom end of the embedded sealing cavity 101 and extend downwards, sealing rings are coated on the outer side walls of the top ends of the outer culture dish 2 and the waste liquid dish 3, the inner side walls of the embedded sealing cavity 101 and the annular sealing cover 11 are respectively hermetically connected with two sealing rings which are distributed from top to bottom, the top ends of the outer culture dish 2 and the waste liquid dish 3 are both, the liquid storage dish 1, the outer culture dish 2 and the waste liquid dish 3 are embedded layer by layer to be sealed and connected, and the liquid storage dish 1 is provided with a sealing cover, so that the pollution-free property of cell culture is effectively realized.

Referring to fig. 4, a plurality of first drainage tubes 4 are fixedly inserted downwards at the inner bottom of a liquid storage dish 1 along the axis direction, a first valve 6 is installed at the top end of the first drainage tubes 4, a first water absorbing corduroy 5 is sleeved inside the first drainage tubes 4, the first water absorbing corduroy 5 extends downwards into an inner culture dish 7, a plurality of second drainage tubes 8 are inserted downwards at the side wall of the inner culture dish 7 along the axis direction, a second valve 10 is installed at the top end of the second drainage tubes 8, a second water absorbing corduroy 9 is sleeved inside the second drainage tubes 8, the second water absorbing corduroy 9 extends downwards into a waste liquid dish 3, a drain pipe 13 is arranged on the side wall of the waste liquid dish 3, an annular cavity for installing the second drainage tubes 8 is reserved between the inner culture dish 7 and the outer culture dish 2, the top end of the second drainage tubes 8 is of a bent pipe structure, the culture liquid of the inner culture dish 7 is easily drained downwards.

Referring to fig. 2 and 5, the inner wall of the waste liquid container 3 is fixedly provided with an accelerating drainage mechanism 12 corresponding to the position of the second water absorption down rope 9, a certain space is left between the accelerating drainage mechanism 12 and the bottom of the waste liquid container 3, and the bottom end of the second water absorption down rope 9 is connected with the accelerating drainage mechanism 12.

Concretely, drainage mechanism 12 includes honeycomb duct 121 on the waste liquid ware 3 inner wall through mount pad 124 fixed connection with higher speed, honeycomb duct 121 keeps away from the powerful sponge 122 that absorbs water of fixedly connected with on the one side inner wall of mount pad 124, the inside fixed mounting of mount pad 124 has electric telescopic handle, electric telescopic handle's flexible end runs through honeycomb duct 121 and fixedly connected with and the powerful extrusion board 123 that absorbs water the sponge 122 position and correspond, the bottom that the fine hair rope 9 was absorbed to the second links up the setting with the top of powerful sponge 122 that absorbs water mutually, at the in-process of drainage, extrusion board 123 can constantly extrude powerful sponge 122 that absorbs water, extrude the culture solution in the powerful sponge 122 that absorbs water, effectively improve the adsorption efficiency of powerful sponge 122 that absorbs water, thereby accelerate the change of old culture solution.

The utility model provides a cell culture device is when using, the cell inoculation is in interior culture dish 7, be equipped with the culture plate that is used for cell culture in interior culture dish 7, this is prior art, add appropriate amount culture medium, add sufficient reserve culture medium in the stock solution dish 1, with the stock solution dish 1, outer culture dish 2, the connection is established to detain each other from top to bottom in waste liquid dish 3, the setting is regularly changed the culture solution, this process is controllable through the timer, the timer can be connected with first valve 6, second valve 10, in order to realize opening and closing of first valve 6 and second valve 10 in the specified time, technical staff in the art can carry out many times experiments, in order to obtain better liquid change time interval, provide reasonable data foundation for the use of device;

when the culture solution is replaced, the second valve 10 is opened, the first valve 6 is closed, at the moment, the culture solution in the inner culture dish 7 is drained outwards through the second water absorption wool 9 in the second drainage tube 8 and is guided into the waste liquid dish 3, the bottom end of the second water absorption wool 9 is connected with the strong water absorption sponge 122 on the accelerated drainage mechanism 12, and flows into the waste liquid dish 3 through the strong water absorption sponge 122 under the action of gravity by utilizing the capillary phenomenon, so that the culture solution in the inner culture dish 7 is drained out, in order to improve the drainage efficiency, the squeezing plate 123 can continuously squeeze the strong water absorption sponge 122 in the drainage process, squeeze the culture solution in the strong water absorption sponge 122 out, effectively improve the adsorption capacity of the strong sponge 122, and further accelerate the replacement of the old culture solution;

after the old culture solution is replaced, the second valve 10 is closed, the first valve 6 is opened, and similarly, the new culture solution in the liquid storage dish 1 is introduced into the inner culture dish 7 through the first water absorption wool 5, the bottom end of the first water absorption wool 5 is submerged in the culture solution in the culture dish 7, therefore, the replacement of the culture solution in the inner culture dish 7 is realized, the first water absorption downy rope 5 and the second water absorption downy rope 9 are both made of water absorption downy fiber materials and have high water absorption capacity, and technicians in the field can set the thickness of the first water absorption downy rope 5 and the second water absorption downy rope 9 according to actual needs, compared with the manual extraction replacement mode in the prior art, the rapid capillary drainage mode of the tissue is adopted, the replacement mode is more moderate, and no adverse effect is caused to cells, in order to improve the efficiency of the accelerated drainage into the inner culture dish 7, a plurality of sets of first water-absorbing corduroy 5 can be provided.

Example 2:

the difference between this embodiment and embodiment 1 is that, after the first valve 6 and the second valve 10 are removed, the first water absorption down ropes 5 and the second water absorption down ropes 9 are always in the drainage environment, which can realize that the culture solution is always stored in the inner culture dish 7 and the cells are always in the condition of sufficient nutrition compared with embodiment 1.

The components adopted in the utility model are all universal standard components or components known by the technicians in the field, and the structure and the principle of the components can be known by the technicians through technical manuals or conventional experimental methods.

The above; is only a preferred embodiment of the present invention; however, the scope of protection of the present invention is not limited thereto; any person skilled in the art is within the technical scope of the present disclosure; according to the technical scheme of the utility model and the improvement conception, equivalent substitution or change is carried out; are all covered by the protection scope of the utility model.

Claims

1. The utility model provides an automatic trade cell culture device of liquid, includes from the top down and inlays in proper order and establishes stock solution dish (1), outer culture dish (2) and waste liquid dish (3) of connecting, outer culture dish (2) are embedded to be established and are connected with interior culture dish (7), its characterized in that: a plurality of first drainage tubes (4) are fixedly inserted downwards at the inner bottom of the liquid storage dish (1) along the axis direction, a first valve (6) is installed at the top end of each first drainage tube (4), a first water absorption wool rope (5) is sleeved inside each first drainage tube (4), and the first water absorption wool ropes (5) extend downwards into the inner culture dish (7);

the lateral wall of interior culture dish (7) is followed its axle center direction and is run through downwards to be inserted and is equipped with a plurality of second drainage tubes (8), and is a plurality of second valve (10) are installed to the top portion of second drainage tube (8), and is a plurality of the inside cover of second drainage tube (8) is equipped with second fine hair rope (9) that absorbs water, and in a plurality of second fine hair rope (9) downwardly extending to waste liquid dish (3), be equipped with blow off pipe (13) on one side lateral wall of waste liquid dish (3), all fixed mounting has accelerated drainage mechanism (12) that correspond with second fine hair rope (9) position that absorb water on the inner wall all around of waste liquid dish (3), and the bottom of second fine hair rope (9) that absorbs water links up the setting with accelerated drainage mechanism (12) mutually.

2. The cell culture apparatus of claim 1, wherein: the bottom of liquid storage ware (1) is equipped with inlays of linking up with outer culture dish (2) top seal and establishes sealed chamber (101), the fixed cover in bottom portion of outer culture dish (2) is equipped with annular sealed lid (11) that link up with waste liquid dish (3) top seal, and is a plurality of first drainage tube (4) run through to inlay the bottom of establishing sealed chamber (101) and extend downwards.

3. The cell culture apparatus of claim 2, wherein: the utility model discloses a culture dish, including outer culture dish (2) and waste liquid ware (3), the top lateral wall of outer culture dish (2) and waste liquid ware (3) is gone up the cladding and is had the sealing washer, inlay the inside wall of establishing sealed chamber (101) and annular sealing lid (11) and respectively with two sealing washer seal joint that distribute from top to bottom.

4. The cell culture apparatus of claim 1, wherein: an annular cavity used for installing a second drainage tube (8) is reserved between the inner culture dish (7) and the outer culture dish (2), and the top end part of the second drainage tube (8) is of a bent pipe structure.

5. The cell culture apparatus of claim 1, wherein: drainage mechanism (12) include honeycomb duct (121) through mount pad (124) fixed connection on waste liquid dish (3) inner wall with higher speed, fixedly connected with powerful sponge (122) that absorbs water on the one side inner wall of keeping away from mount pad (124) of honeycomb duct (121), the inside fixed mounting of mount pad (124) has electric telescopic handle, electric telescopic handle's flexible end runs through honeycomb duct (121) and fixedly connected with and the corresponding stripper plate (123) of powerful sponge (122) position that absorbs water.

6. The cell culture apparatus of claim 5, wherein: the bottom end of the second water absorption wool rope (9) is connected with the top end of the strong water absorption sponge (122).