CN205035388U - Cell culture dish for experiments - Google Patents

Abstract

The utility model discloses an experimental cell culture dish, which belongs to the technical field of cell culture, and comprises a dish body and a dish cover, and is characterized in that it also includes an elastic connecting rod, which consists of a first connecting rod section, a spring section and a second connecting rod section. The two connecting rod sections are connected end to end in turn. There is a transparent baffle inside the dish, and a connecting piece is fixed at the center of the transparent baffle. The first connecting rod section is connected to the connecting piece, and the second connecting rod section is slidably connected to the dish cover. , a clamping block for clamping the second connecting rod segment is fixed on the dish cover, an anti-fog film is bonded on the inner wall of the dish cover, a unidirectional air guide tube is connected to the outer wall of the dish cover, and the unidirectional air guide The trachea extends into the dish body. The utility model adopts the structure of the transparent baffle, and the experimenter can observe the changes of the cells in the culture solution from the top of the culture dish at any time. Even if the transparent baffle is in contact with the culture solution for a long time, it is not easy to be soaked and damaged, and bacteria will not be produced , to ensure the effect of the culture medium and improve the success rate of cell culture.

Description

An experimental cell culture dish

technical field

The utility model relates to the technical field of cell culture, in particular to a cell culture dish for experiments.

Background technique

The growth of cells requires a nutrient environment, and the nutrient matrix used to maintain cell growth is called a medium. The medium can be divided into liquid medium and solid medium according to its physical state. Liquid culture medium is used in large-scale industrial production and research on basic theories such as physiological metabolism. A certain coagulant, such as agar, is added to the liquid medium, or a solid culture, such as bran, rice, etc., becomes a solid medium. Solid media provide a nutrient and aerated surface for cell growth, and cells produced on such a nutrient surface form single colonies. Therefore, solid medium plays a very important role in the separation, identification and counting of cells. Isolation of required cells and expanded cells from multicellular organisms and in vitro transformation of cells, observation, must solve the problem of cell culture in vitro. Compared with the difficulty of microbial cell culture, it is more difficult to come from multicellular organisms Single cell culture, especially animal cell culture. Cell culture generally refers to all in vitro cultures, which means taking tissues from living animals and incubating them under specific in vivo conditions such as simulating the physiological environment in vivo to make them survive and grow. Cell culture has been widely used in various fields such as biology, medicine, and new drug research and development, and has become one of the most important basic sciences. In the process of cell culture, the equipment that needs to be used is a cell culture dish, which is a laboratory vessel for cell culture, consisting of a flat disc-shaped bottom and a cover. The material of the petri dish is basically divided into two categories, mainly plastic and glass, and the glass can be used for the adherent culture of plant materials and animal cells. There are disposable and multiple-use plastics, which are suitable for laboratory inoculation, streaking, and isolation of bacteria, and are mainly used for the cultivation of plant materials.

The publication number is CN204281760U, and the Chinese patent literature published on April 22, 2015 discloses a cell culture dish, which is characterized in that it mainly includes a culture dish cover, a fixed support, an omentum and a culture dish bottom, and the culture dish The bottom is a hollow cylinder with an open top, and the petri dish cover is a hollow cylinder with an open bottom. The inner diameter of the petri dish cap is larger than the outer diameter of the bottom of the petri dish. There are many connecting strips, the omentum is located at the bottom of the fixed bracket and fixed on the multiple connecting strips, there is a distance between the bottom surface of each connecting strip and the omentum, and the omentum, the multiple connecting strips and the fixed bracket Located in the bottom of the petri dish. In the cell culture dish disclosed in this patent document, the omentum is fixed by a plurality of connecting bars at the bottom of the fixed support, and the tissue block is limited in the culture solution by the space formed between the omentum and the bottom of the dish. With this structure, although To a certain extent, it can prevent the tissue block from floating, but there are the following defects:

1. Due to the occlusion of the omentum, the experimenter cannot observe the changes of the cells in the culture medium in real time from directly above the culture dish, which affects the culture efficiency and effect of the cells.

2. Due to the use of a ring-shaped fixed bracket, during the cell culture process, it is necessary to open the lid of the dish every time the cells are taken out and take out the fixed bracket to take out the cells in the bottom of the dish. Not only is the operation cumbersome, but also the culture medium Exposure to the air will easily affect the effect of the culture medium, thereby affecting the cultivation of cells.

3. To prevent the tissue blocks from floating, the omentum must be in contact with the culture medium for a long time. The omentum is easily soaked and damaged, thereby producing bacteria, affecting the culture medium, and directly reducing the success rate of cell culture.

Utility model content

In order to overcome the defects of the above-mentioned prior art, the utility model provides a cell culture dish for experiments. The utility model adopts the structure of a transparent baffle, and the experimenter can observe the changes of the cells in the culture medium from directly above the culture dish at any time. Even if the transparent baffle is in contact with the culture medium for a long time, it will not be easily soaked and damaged, and will not produce bacteria, which ensures the effect of the culture medium and improves the success rate of cell culture.

The utility model is realized through the following technical solutions:

An experimental cell culture dish, comprising a dish body and a dish cover connected to the dish body, is characterized in that: it also includes an elastic connecting rod, the elastic connecting rod is a three-section split structure, and the elastic connecting rod is composed of a first The connecting rod section, the spring section and the second connecting rod section are connected end to end in sequence. A transparent baffle is arranged inside the vessel, and a connecting piece is fixed at the center of the transparent baffle. The first connecting rod section is connected to the The second connecting rod section is slidably connected to the vessel cover, the clamping block for clamping the second connecting rod section is fixed on the vessel cover, and the anti-fog film is glued on the inner wall of the vessel cover , the outer wall of the vessel cover is connected with a one-way air guide tube, and the one-way air guide tube extends into the vessel body.

The dish body is provided with a plurality of cell-carrying chambers for placing cells, and the cell-carrying chambers are detachably connected to the inner bottom wall of the dish body, and the plurality of cell-carrying chambers are evenly distributed in a circular shape.

A diversion groove is also provided in the dish body, the diversion groove is located between two adjacent cell-carrying chambers, and the diversion groove communicates with the two cell-carrying chambers respectively.

A plurality of circular through holes are opened on the dish cover, and the plurality of circular through holes are evenly distributed on the dish cover in a ring shape. The circular through holes correspond to the cell-carrying chambers, and sealing plugs are embedded in the circular through holes.

The dish body is provided with a concave buffer seat, and the concave buffer seat is covered on the outer bottom wall of the dish body.

The dish body and the dish cover are clamped or threaded, and the connection between the dish body and the dish cover is covered with an outer sheath.

The beneficial effects of the utility model are mainly manifested in the following aspects:

1. In the present utility model, the elastic connecting rod is a three-section split structure. The elastic connecting rod is connected end to end by the first connecting rod segment, the spring segment and the second connecting rod segment. A connecting piece is fixed at the center of the transparent baffle, the first connecting rod section is connected to the connecting piece, the second connecting rod section is slidably connected to the dish cover, and a clamping block for clamping the second connecting rod section is fixed on the dish cover , the inner wall of the dish cover is bonded with an anti-fog film, and the outer wall of the dish cover is connected with a one-way air guide tube, which extends into the dish body. The baffle can observe the changes of cells in the culture medium in real time, and take corresponding culture measures in a timely manner, so as to improve the efficiency and effect of cell culture; , it is not easy to be soaked and damaged, and will not produce bacteria, which ensures the effect of the culture medium and improves the success rate of cell culture; through the cooperation of the three-section elastic connecting rod and the clamping block, it is very convenient to adjust the transparent baffle in the dish The position in the vertical direction, even if the culture medium is added during the cell culture process, the transparent baffle can limit the cells in the culture medium to ensure the effect of cell culture. In addition, the elastic connecting rod has a certain buffering effect, which can effectively prevent The transparent baffle and the inner wall of the dish will cause greater friction and damage the dish body; the inner wall of the dish cover is bonded with an anti-fog film, and the outer wall of the dish cover is connected with a one-way air guide tube, which extends into the dish body, and the cell culture During the process, when the lid of the dish is opened and the culture medium is added, the air is easy to enter the dish, and due to the change of the ambient temperature, the inside of the dish is prone to fogging, and the anti-fog film can prevent foggy water droplets from being generated in the dish, which is convenient for cell observation. It can also discharge part of the air in the dish. The combination of the two can completely prevent fogging in the dish, and the experimenter can observe the cells more clearly through the dish, which greatly improves the cell culture effect.

2. In the utility model, a plurality of cell-carrying chambers for placing cells are arranged in the dish body, and the cell-carrying chambers are detachably connected to the bottom wall of the dish body, and the plurality of cell-carrying chambers are evenly distributed in a circular shape, which can Cultivate a variety of cells at the same time. When you want to observe a certain cell, you can directly take out the cell carrier chamber. It is very convenient for observation and research. Multiple cell carrier chambers are evenly distributed in a circular shape and can be observed from the side wall of the dish. The condition of the cells in each cell carrier chamber will not cross and overlap, which is more conducive to cell culture.

3. In the utility model, a diversion groove is also arranged in the dish body, and the diversion groove is located between two adjacent cell-carrying chambers, and the diversion groove is respectively connected with the two cell-carrying chambers, and the two cell-carrying chambers can be connected through the diversion groove. Cells in adjacent cell-carrying chambers move with each other, which facilitates the study of cell-cell interactions and facilitates better cell culture.

4. In the utility model, a plurality of circular through holes are opened on the dish cover, and the plurality of circular through holes are evenly distributed on the dish cover in a ring shape. There is a sealing plug. During the cell culture process, when a certain type of cell is to be taken out for observation, you only need to open the sealing plug on the corresponding circular through hole to take out the cell-carrying chamber. The operation is quite simple and can effectively prevent the culture medium from leaking. A large area is exposed to the air to ensure the effect of the culture medium, thereby increasing the culture rate of the cells.

5. In the utility model, the dish body is provided with a concave buffer seat, and the concave buffer seat is covered on the outer bottom wall of the dish body. During the cell culture process, when the dish body is moved, the concave buffer seat can play a certain role. The buffering effect prevents the culture medium in the dish from being greatly shaken to affect the cells, which is beneficial to ensure the normal culture of the cells.

6. In the utility model, the dish body and the dish cover are clamped or threaded, and the connection between the dish body and the dish cover is covered with an outer sheath. By adding an outer sheath, the connection strength between the dish body and the dish cover can be enhanced. Prevent the culture medium from spilling during transportation, and ensure that the cells are fully immersed in the culture medium.

Description of drawings

Fig. 1 is the structural representation of the utility model;

Fig. 2 is the sectional view of the utility model dish cover;

Fig. 3 is the structural representation of the utility model embodiment 2;

Fig. 4 is the structural representation of the utility model embodiment 3;

Fig. 5 is the structural representation of the utility model embodiment 4;

Fig. 6 is the structural representation of the utility model embodiment 5;

Fig. 7 is the structural representation of the utility model embodiment 6;

Marks in the figure: 1. Dish body, 2. Dish cover, 3. First connecting rod section, 4. Spring section, 5. Second connecting rod section, 6. Transparent stopper, 7. Connecting piece, 8. Clamping Block, 9, cell carrying cavity, 10, diversion groove, 11, circular through hole, 12, sealing plug, 13, concave buffer seat, 14, outer sheath, 15, anti-fog film, 16, one-way air guide tube .

detailed description

Example 1

Referring to Figures 1 and 2, a cell culture dish for experiments includes a dish body 1 and a dish cover 2 connected to the dish body 1, and also includes an elastic connecting rod, which is a three-stage split structure, The elastic connecting rod is formed by connecting the first connecting rod section 3, the spring section 4 and the second connecting rod section 5 sequentially end to end, and a transparent baffle 6 is arranged inside the dish body 1, and the center of the transparent baffle 6 is fixed There is a connecting piece 7, the first connecting rod section 3 is connected to the connecting piece 7, the second connecting rod section 5 is slidably connected to the dish cover 2, and the dish cover 2 is fixed with a connecting rod for clamping the second connecting rod The clamping block 8 of section 5, the inner wall of the dish cover 2 is bonded with an anti-fog film 15, and the outer wall of the dish cover 2 is connected with a one-way air guide tube 16, and the one-way air guide tube 16 extends into the dish body 1 Inside.

This embodiment is the most basic implementation mode. The elastic connecting rod is a three-section split structure. The elastic connecting rod is connected end to end by the first connecting rod segment, the spring segment and the second connecting rod segment in sequence. The baffle, the center of the transparent baffle is fixed with a connecting piece, the first connecting rod section is connected with the connecting piece, the second connecting rod section is slidably connected on the dish cover, and the dish cover is fixed with a clamp for clamping the second connecting rod section The clamping block, the inner wall of the dish cover is bonded with an anti-fog film, the outer wall of the dish cover is connected with a one-way air guide tube, and the one-way air guide tube extends into the inside of the dish. The changes of cells in the culture medium can be observed in real time through the transparent baffle, and corresponding culture measures can be taken in a timely manner, so as to improve the efficiency and effect of cell culture; It is not easy to be soaked and damaged in contact with the culture medium, and will not produce bacteria, ensuring the effect of the culture medium and improving the success rate of cell culture; through the cooperation of the three-section elastic connecting rod and the clamping block, the transparent baffle can be adjusted very conveniently In the vertical direction of the dish, even if the culture medium is added during the cell culture process, the transparent baffle can limit the cells in the culture medium to ensure the effect of cell culture. In addition, the elastic connecting rod has a certain buffering effect, It can effectively prevent the large friction between the transparent baffle and the inner wall of the vessel and damage the vessel body; the inner wall of the vessel cover is bonded with an anti-fog film, and the outer wall of the vessel cover is connected with a one-way air guide tube, which extends into the vessel body , During the cell culture process, when the lid of the dish is opened and the culture medium is added, the air easily enters the dish body, and due to the change of the ambient temperature, the dish body is prone to fogging, and the anti-fog film can prevent the foggy water droplets from being generated in the dish body, which is convenient for cell observation. The one-way air guide tube can also discharge part of the air in the dish. The combination of the two can completely prevent fogging in the dish, and the experimenter can observe the cells more clearly through the dish, which greatly improves the cell culture effect.

Example 2

Referring to Figures 2 and 3, a cell culture dish for experiments includes a dish body 1 and a dish cover 2 connected to the dish body 1, and also includes an elastic connecting rod, which is a three-stage split structure, The elastic connecting rod is formed by connecting the first connecting rod section 3, the spring section 4 and the second connecting rod section 5 sequentially end to end, and a transparent baffle 6 is arranged inside the dish body 1, and the center of the transparent baffle 6 is fixed There is a connecting piece 7, the first connecting rod section 3 is connected to the connecting piece 7, the second connecting rod section 5 is slidably connected to the dish cover 2, and the dish cover 2 is fixed with a connecting rod for clamping the second connecting rod The clamping block 8 of section 5, the inner wall of the dish cover 2 is bonded with an anti-fog film 15, and the outer wall of the dish cover 2 is connected with a one-way air guide tube 16, and the one-way air guide tube 16 extends into the dish body 1 Inside.

The dish body 1 is provided with a plurality of cell-carrying chambers 9 for placing cells, and the cell-carrying chambers 9 are detachably connected to the inner bottom wall of the dish body 1, and the plurality of cell-carrying chambers 9 are evenly distributed in a circular shape .

This embodiment is a preferred implementation mode. There are multiple cell-carrying chambers for placing cells in the dish. The cell-carrying chambers are detachably connected to the bottom wall of the dish body. The multiple cell-carrying chambers are circular and uniform. Distributed, can cultivate a variety of cells at the same time, when you want to observe a certain cell, you can directly take out the cell cavity, which is very convenient for observation and research, multiple cell cavity is evenly distributed in a ring shape, and can be obtained from the dish body The side wall observes the condition of the cells in each cell cavity, and will not cross and overlap, which is more conducive to cell culture.

Example 3

Referring to Figures 2 and 4, a cell culture dish for experiments includes a dish body 1 and a dish cover 2 connected to the dish body 1, and also includes an elastic connecting rod, which is a three-stage split structure, The elastic connecting rod is formed by connecting the first connecting rod section 3, the spring section 4 and the second connecting rod section 5 sequentially end to end, and a transparent baffle 6 is arranged inside the dish body 1, and the center of the transparent baffle 6 is fixed There is a connecting piece 7, the first connecting rod section 3 is connected to the connecting piece 7, the second connecting rod section 5 is slidably connected to the dish cover 2, and the dish cover 2 is fixed with a connecting rod for clamping the second connecting rod The clamping block 8 of section 5, the inner wall of the dish cover 2 is bonded with an anti-fog film 15, and the outer wall of the dish cover 2 is connected with a one-way air guide tube 16, and the one-way air guide tube 16 extends into the dish body 1 Inside.

The dish body 1 is provided with a plurality of cell-carrying chambers 9 for placing cells, and the cell-carrying chambers 9 are detachably connected to the inner bottom wall of the dish body 1, and the plurality of cell-carrying chambers 9 are evenly distributed in a circular shape .

The dish body 1 is also provided with a diversion groove 10 , the diversion groove 10 is located between two adjacent cell-carrying chambers 9 , and the diversion groove 10 communicates with the two cell-carrying chambers 9 respectively.

This embodiment is yet another preferred implementation mode. A diversion groove is also provided in the dish, and the diversion groove is located between two adjacent cell-carrying chambers. The diversion groove is respectively connected with the two cell-carrying chambers. It can make the cells in two adjacent cell-carrying chambers move with each other, which is conducive to the study of the interaction between cells and better cell culture.

Example 4

Referring to Figure 2 and Figure 5, a cell culture dish for experiments includes a dish body 1 and a dish cover 2 connected to the dish body 1, and also includes an elastic connecting rod, which is a three-stage split structure, The elastic connecting rod is formed by connecting the first connecting rod section 3, the spring section 4 and the second connecting rod section 5 sequentially end to end, and a transparent baffle 6 is arranged inside the dish body 1, and the center of the transparent baffle 6 is fixed There is a connecting piece 7, the first connecting rod section 3 is connected to the connecting piece 7, the second connecting rod section 5 is slidably connected to the dish cover 2, and the dish cover 2 is fixed with a connecting rod for clamping the second connecting rod The clamping block 8 of section 5, the inner wall of the dish cover 2 is bonded with an anti-fog film 15, and the outer wall of the dish cover 2 is connected with a one-way air guide tube 16, and the one-way air guide tube 16 extends into the dish body 1 Inside.

The dish body 1 is provided with a plurality of cell-carrying chambers 9 for placing cells, and the cell-carrying chambers 9 are detachably connected to the inner bottom wall of the dish body 1, and the plurality of cell-carrying chambers 9 are evenly distributed in a circular shape .

The dish body 1 is also provided with a diversion groove 10 , the diversion groove 10 is located between two adjacent cell-carrying chambers 9 , and the diversion groove 10 communicates with the two cell-carrying chambers 9 respectively.

The dish cover 2 is provided with a plurality of circular through holes 11, and the plurality of circular through holes 11 are evenly distributed on the dish cover 2 in a ring shape. A sealing plug 12 is embedded in the hole 11 .

This embodiment is yet another preferred implementation mode. There are a plurality of circular through holes on the dish cover, and the plurality of circular through holes are evenly distributed on the dish cover in a ring shape. The through hole is embedded with a sealing plug. During the cell culture process, when a certain type of cell is to be taken out for observation, only the sealing plug on the corresponding circular through hole can be opened to take out the cell-carrying chamber. The operation is quite simple and can Effectively prevent the culture medium from being exposed to the air in a large area, ensure the effect of the culture medium, and increase the culture rate of the cells.

Example 5

Referring to Figure 2 and Figure 6, a cell culture dish for experiments includes a dish body 1 and a dish cover 2 connected to the dish body 1, and also includes an elastic connecting rod, which is a three-stage split structure, The elastic connecting rod is formed by connecting the first connecting rod section 3, the spring section 4 and the second connecting rod section 5 sequentially end to end, and a transparent baffle 6 is arranged inside the dish body 1, and the center of the transparent baffle 6 is fixed There is a connecting piece 7, the first connecting rod section 3 is connected to the connecting piece 7, the second connecting rod section 5 is slidably connected to the dish cover 2, and the dish cover 2 is fixed with a connecting rod for clamping the second connecting rod The clamping block 8 of section 5, the inner wall of the dish cover 2 is bonded with an anti-fog film 15, and the outer wall of the dish cover 2 is connected with a one-way air guide tube 16, and the one-way air guide tube 16 extends into the dish body 1 Inside.

The dish body 1 is provided with a plurality of cell-carrying chambers 9 for placing cells, and the cell-carrying chambers 9 are detachably connected to the inner bottom wall of the dish body 1, and the plurality of cell-carrying chambers 9 are evenly distributed in a circular shape .

The dish body 1 is also provided with a diversion groove 10 , the diversion groove 10 is located between two adjacent cell-carrying chambers 9 , and the diversion groove 10 communicates with the two cell-carrying chambers 9 respectively.

The dish cover 2 is provided with a plurality of circular through holes 11, and the plurality of circular through holes 11 are evenly distributed on the dish cover 2 in a ring shape. A sealing plug 12 is embedded in the hole 11 .

The dish body 1 is provided with a concave buffer seat 13 , and the concave buffer seat 13 is sleeved on the outer bottom wall of the dish body 1 .

This embodiment is yet another preferred implementation mode. The dish body is provided with a concave buffer seat, and the concave buffer seat is covered on the outer bottom wall of the dish body. During the cell culture process, when the dish body is moved, the concave buffer seat It can play a certain buffering role, prevent the culture medium in the dish from being greatly shaken and affect the cells, and is beneficial to ensure the normal culture of the cells.

Example 6

Referring to Figure 2 and Figure 7, a cell culture dish for experiments includes a dish body 1 and a dish cover 2 connected to the dish body 1, and also includes an elastic connecting rod, which is a three-stage split structure, The elastic connecting rod is formed by connecting the first connecting rod section 3, the spring section 4 and the second connecting rod section 5 sequentially end to end, and a transparent baffle 6 is arranged inside the dish body 1, and the center of the transparent baffle 6 is fixed There is a connecting piece 7, the first connecting rod section 3 is connected to the connecting piece 7, the second connecting rod section 5 is slidably connected to the dish cover 2, and the dish cover 2 is fixed with a connecting rod for clamping the second connecting rod The clamping block 8 of section 5, the inner wall of the dish cover 2 is bonded with an anti-fog film 15, and the outer wall of the dish cover 2 is connected with a one-way air guide tube 16, and the one-way air guide tube 16 extends into the dish body 1 Inside.

The dish body 1 is provided with a plurality of cell-carrying chambers 9 for placing cells, and the cell-carrying chambers 9 are detachably connected to the inner bottom wall of the dish body 1, and the plurality of cell-carrying chambers 9 are evenly distributed in a circular shape .

The dish body 1 is also provided with a diversion groove 10 , the diversion groove 10 is located between two adjacent cell-carrying chambers 9 , and the diversion groove 10 communicates with the two cell-carrying chambers 9 respectively.

The dish cover 2 is provided with a plurality of circular through holes 11, and the plurality of circular through holes 11 are evenly distributed on the dish cover 2 in a ring shape. A sealing plug 12 is embedded in the hole 11 .

The dish body 1 is provided with a concave buffer seat 13 , and the concave buffer seat 13 is sleeved on the outer bottom wall of the dish body 1 .

The dish body 1 and the dish cover 2 are clamped or threadedly connected, and the joint between the dish body 1 and the dish cover 2 is covered with an outer sheath 14 .

This embodiment is the best implementation mode. The elastic connecting rod is a three-section split structure. The elastic connecting rod is formed by connecting the first connecting rod segment, the spring segment and the second connecting rod segment sequentially. A connecting piece is fixed at the center of the transparent baffle, the first connecting rod section is connected with the connecting piece, the second connecting rod section is slidably connected to the dish cover, and a clip for clamping the second connecting rod section is fixed on the dish cover The inner wall of the dish cover is bonded with an anti-fog film, and the outer wall of the dish cover is connected with a one-way air guide tube. The one-way air guide tube extends into the inside of the dish. Observe the changes of cells in the culture medium in real time through the transparent baffle, and take corresponding culture measures in time, so as to improve the culture efficiency and effect of cells; liquid contact, it is not easy to be soaked and damaged, and will not produce bacteria, which ensures the effect of the culture medium and improves the success rate of cell culture; through the cooperation of the three-section elastic connecting rod and the clamping block, it is very convenient to adjust the transparent baffle in the The position in the vertical direction of the dish body, even if the culture medium is added during the cell culture process, the transparent baffle can also limit the cells in the culture medium to ensure the cell culture effect. In addition, the elastic connecting rod has a certain buffering effect, which can Effectively prevent the large friction between the transparent baffle and the inner wall of the vessel and damage the vessel body; the inner wall of the vessel cover is bonded with an anti-fog film, and the outer wall of the vessel cover is connected with a one-way air guide tube, which extends into the vessel body. During the cell culture process, when the lid of the dish is opened and the culture medium is added, the air easily enters the dish, and due to the change of the ambient temperature, the inside of the dish is prone to fogging, and the anti-fog film can prevent the foggy water droplets in the dish, which is convenient for cell observation. The air guide tube can also discharge part of the air in the dish. The combination of the two can completely prevent fogging in the dish, and the experimenter can observe the cells more clearly through the dish, which greatly improves the cell culture effect. The dish body and the dish cover are clamped or threaded, and the connection between the dish body and the dish cover is covered with an outer sheath. By adding an outer sheath, the connection strength between the dish body and the dish cover can be enhanced to prevent the culture liquid from spilling during transportation. Make sure that the cells are fully immersed in the culture medium.

Claims (6)

1. an experiment Tissue Culture Dish, comprise ware body (1) and be connected to the ware lid (2) on ware body (1), it is characterized in that: also comprise flexible connecting rod, described flexible connecting rod is syllogic Split type structure, flexible connecting rod is by first connecting rod section (3), spring section (4) and second connecting rod section (5) successively head and the tail are formed by connecting, transparent catch (6) is provided with in described ware body (1), the center of described transparent catch (6) is fixed with connection piece (7), described first connecting rod section (3) is connected with connection piece (7), described second connecting rod section (5) is slidably connected on ware lid (2), described ware lid (2) is fixed with the grip block (8) for clamping second connecting rod section (5), the inwall of described ware lid (2) is bonded with antifogging film (15), the outer wall of described ware lid (2) is connected with unidirectional tracheae (16), described unidirectional tracheae (16) is stretched in ware body (1).

2. a kind of experiment Tissue Culture Dish according to claim 1, it is characterized in that: be provided with multiple cell for placing cell in described ware body (1) and carry chamber (9), cell carries on the detachable interior diapire being connected to ware body (1) in chamber (9), and multiple cell carries chamber (9) and is uniformly distributed in circular.

3. a kind of experiment Tissue Culture Dish according to claim 2, it is characterized in that: in described ware body (1), be also provided with diversion trench (10), diversion trench (10) is positioned at two flanking cells and carries between chamber (9), and diversion trench (10) carries chamber (9) with two cells respectively and is communicated with.

4. a kind of experiment Tissue Culture Dish according to claim 3, it is characterized in that: described ware lid (2) has multiple manhole (11), multiple manhole (11) is evenly distributed on ware lid (2) in the form of a ring, it is corresponding that manhole (11) and cell carry chamber (9), manhole (11) is embedded with sealing plug (12).

5. a kind of experiment Tissue Culture Dish according to claim 4, is characterized in that: described ware body (1) is provided with spill buffing pad (13), and spill buffing pad (13) is enclosed within the exterior bottom wall of ware body (1).

6. a kind of experiment Tissue Culture Dish according to claim 1 or 5, it is characterized in that: described ware body (1) and ware lid (2) are for clamping or be threaded, the junction of ware body (1) and ware lid (2) is overlapped and is had oversheath (14).