CN205035388U - Cell culture dish for experiments - Google Patents
Cell culture dish for experiments Download PDFInfo
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- CN205035388U CN205035388U CN201520773669.4U CN201520773669U CN205035388U CN 205035388 U CN205035388 U CN 205035388U CN 201520773669 U CN201520773669 U CN 201520773669U CN 205035388 U CN205035388 U CN 205035388U
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Abstract
The utility model discloses a cell culture dish for experiments belongs to the cell culture technique field, including dish body and ware lid, its characterized in that: still include elastic connecting rod, elastic connecting rod is formed by a connecting rod section, spring section and the 2nd connecting rod section end -to -end connection in proper order, is provided with transparent separation blade in the dish body, and the center department of transparent separation blade is fixed with the connection piece, and a connecting rod section is connected with the connection piece, and the 2nd connecting rod section sliding connection covers at the ware, and the ware is covered and is fixed with the grip block that is used for the 2nd connecting rod section of centre gripping, it has antifog coating film to bond on the inner wall of ware lid, be connected with one -way air duct on the outer wall of ware lid, one -way air duct stretches into in the dish body. The utility model discloses a structure of transparent separation blade, the laboratory technician can observe the change of cell in the culture solution at any time directly over the culture dish, even if transparent separation blade is long -time and the culture solution contact, also be difficult to soak the damage, can not produce the bacterium, guarantees the effect of culture solution, improves the cell culture success rate.
Description
Technical field
The utility model relates to technical field of cell culture, particularly relates to a kind of experiment Tissue Culture Dish.
Background technology
The growth needs nutrient environment of cell, is called substratum for the nutraceutical matrix maintaining Growth of Cells.Substratum can be divided into liquid nutrient medium and solid medium by its physical condition.Liquid nutrient medium is used for the research work of the basic theories such as large-scale industrial production and physiological metabolism.Certain peptizer is added, as agar in liquid nutrient medium; Or solid culture, as wheat bran, rice etc., just becomes solid medium.Solid medium is the surface that the growth of cell provides a nutrition and ventilation, and the cell produced on the surface in such nutrition can form single bacterium colony.Therefore, solid medium plays considerable effect in the separation, qualification, counting etc. of cell.Required cell is separated and the cell that obtains and engineered ex vivo is carried out to cell of increasing from multicellular organism, observe, colony-formation assays problem must be solved, the difficulty or ease of cultivating with microorganism cells are compared, the cultivation of more difficult is from multicellular organism single cell culture, particularly zooblast.Cell cultures makes a general reference all vitro culture, and its implication refers to takes out tissue in living animal body, in the specific body such as physiological environment under condition, carries out hatching cultivation, makes it to survive and grow in analogue body.Cell cultures work has now been widely used in the every field such as biology, medical science, new drug development, becomes one of most important basic science.In cell cultivation process, need the equipment used to be Tissue Culture Dish, Tissue Culture Dish is a kind of labware for cell cultures, is made up of the end of a flat circle plate-like and a lid.Culture dish material is divided into two classes substantially, is mainly plastic and glass, the adherent culture that may be used for vegetable material and zooblast of glass.The once property of plastics with nonexpondable, be applicable to the operation of laboratory inoculation, line, separation of bacterial, be mainly used in the cultivation of vegetable material.
Publication number is CN204281760U, publication date is that the Chinese patent literature of on 04 22nd, 2015 discloses a kind of Tissue Culture Dish, it is characterized in that: mainly comprise culture dish lid, fixed support, at the bottom of nethike embrane and culture dish, it is the hollow cylinder of top end opening at the bottom of described culture dish, culture dish lid is the hollow cylinder of bottom end opening, the internal diameter of described culture dish lid is greater than the external diameter at the bottom of culture dish, described fixed support is circular, fixed support is evenly fixed with many connecting strips, described nethike embrane is positioned at support bracket fastened bottom and is fixed on many connecting strips, distance is had between the bottom surface of each connecting strip and nethike embrane, described nethike embrane, many connecting strips and fixed support are all positioned at the bottom of culture dish.Tissue Culture Dish disclosed in this patent documentation, nethike embrane is fixed with by many connecting strips in support bracket fastened bottom, by the space formed between at the bottom of nethike embrane and ware, tissue block is limited in nutrient solution, adopt this structure, although tissue block can be prevented to a certain extent floating, there is following defect:
Blocking one, due to nethike embrane, the change that the observation of cell that laboratory technician can not be real-time directly over culture dish occurs nutrient solution, affects culture efficiency and the effect of cell.
Two, owing to have employed circular fixed support, in cell cultivation process, all need when taking out cell each time to open ware lid, take out fixed support, cell at the bottom of ware could be taken out, not only complex operation, and nutrient solution exposes in atmosphere, the easy effect affecting nutrient solution, thus affect the cultivation of cell.
Three, nethike embrane will stop tissue block floating, and must contact with nutrient solution for a long time, nethike embrane easily soaks damage, thus produces bacterium, affects nutrient solution, directly causes cell cultures success ratio to reduce.
Utility model content
The utility model is in order to overcome the defect of above-mentioned prior art, a kind of experiment Tissue Culture Dish is provided, the utility model adopts the structure of transparent catch, laboratory technician can at any time from the change of observation of cell nutrient solution directly over culture dish, even if transparent catch contacts with nutrient solution for a long time, be also not easy to soak and damage, can not bacterium be produced, ensure the effect of nutrient solution, improve cell cultures success ratio.
The utility model is achieved through the following technical solutions:
A kind of experiment Tissue Culture Dish, comprise ware body and be connected to the ware lid on ware body, it is characterized in that: also comprise flexible connecting rod, described flexible connecting rod is syllogic Split type structure, flexible connecting rod is by first connecting rod section, spring section and second connecting rod section successively head and the tail are formed by connecting, transparent catch is provided with in described ware body, the center of described transparent catch is fixed with connection piece, described first connecting rod section is connected with connection piece, described second connecting rod section is slidably connected at ware and covers, described ware covers the grip block be fixed with for clamping second connecting rod section, the inwall of described ware lid is bonded with antifogging film, the outer wall of described ware lid is connected with unidirectional tracheae, described unidirectional tracheae stretches in ware body.
Be provided with multiple cell for placing cell in described ware body and carry chamber, cell carries that chamber is detachable to be connected on the interior diapire of ware body, and it is circular being uniformly distributed that multiple cell carries chamber.
Also be provided with diversion trench in described ware body, diversion trench carries between chamber at two flanking cells, and diversion trench carries chamber with two cells respectively and is communicated with.
Described ware covers and has multiple manhole, and multiple manhole is evenly distributed on ware in the form of a ring and covers, and it is corresponding that manhole and cell carry chamber, and manhole is embedded with sealing plug.
Described ware body is provided with spill buffing pad, and spill buffing pad is enclosed within the exterior bottom wall of ware body.
Described ware body and ware lid are clamping or are threaded, and the junction of ware body and ware lid is overlapped and had oversheath.
The beneficial effects of the utility model main manifestations is in the following areas:
One, in the utility model, flexible connecting rod is syllogic Split type structure, flexible connecting rod is by first connecting rod section, spring section and second connecting rod section successively head and the tail are formed by connecting, transparent catch is provided with in ware body, the center of transparent catch is fixed with connection piece, first connecting rod section is connected with connection piece, second connecting rod section is slidably connected at ware and covers, ware covers the grip block be fixed with for clamping second connecting rod section, the inwall of ware lid is bonded with antifogging film, the outer wall of ware lid is connected with unidirectional tracheae, unidirectional tracheae stretches in ware body, in cell cultivation process, laboratory technician can at any time directly over culture dish through the change of observation of cell nutrient solution that transparent catch is real-time, take corresponding training measures timely, thus be beneficial to the culture efficiency and culture effect that improve cell, compared to nethike embrane, even if transparent catch contacts with nutrient solution for a long time, be also not easy to soak and damage, can not bacterium be produced, ensure the effect of nutrient solution, improve cell cultures success ratio, by the flexible connecting rod of syllogic and coordinating of grip block, the position of transparent catch in ware body on vertical direction can be adjusted very easily, even if add nutrient solution in cell cultivation process, cell also can be limited in nutrient solution by transparent catch, ensure cell cultures effect, in addition, flexible connecting rod has certain shock absorption, can effectively prevent transparent catch and ware body inwall from producing larger friction and damaging ware body, the inwall of ware lid is bonded with antifogging film, the outer wall of ware lid is connected with unidirectional tracheae, unidirectional tracheae stretches in ware body, in cell cultivation process, when unlatching ware lid adds nutrient solution, air easily enters in ware body, because of the change of envrionment temperature, easily haze in ware body, and antifogging film can prevent from producing in ware body vaporific water droplet, be convenient to the observation of cell, unidirectional tracheae also can discharge the partial air in ware body, the two coordinates, can prevent from completely hazing in ware body, laboratory technician is more clear through ware body observation of cell, greatly improve cell cultures effect.
Two, in the utility model, be provided with multiple cell for placing cell in ware body and carry chamber, cell carries on the detachable diapire being connected to ware body in chamber, it is circular being uniformly distributed that multiple cell carries chamber, can cultivate various kinds of cell simultaneously, when certain cell will be observed, this cell of direct taking-up carries chamber, observational study is quite convenient, it is circular being uniformly distributed that multiple cell carries chamber, the situation that each cell carries chamber inner cell can be observed from the sidewall of ware body, can not juxtaposition, thus be more conducive to the cultivation of cell.
Three, in the utility model, also diversion trench is provided with in ware body, diversion trench carries between chamber at two flanking cells, diversion trench carries chamber with two cells respectively and is communicated with, the cell that two flanking cells carry in chamber can be allowed mutually to move by diversion trench, thus be beneficial to the intercellular interaction of research, be convenient to carry out better cell cultures.
Four, in the utility model, ware covers and has multiple manhole, multiple manhole is evenly distributed on ware in the form of a ring and covers, it is corresponding that manhole and cell carry chamber, manhole is embedded with sealing plug, in cell cultivation process, when a certain cell will be taken out to be observed, only need open the sealing plug on respective circular through hole, just can take out cell and carry chamber, operation is quite simple, can effectively prevent nutrient solution big area from exposing in atmosphere, ensure the effect of nutrient solution, thus improve the cultivation rate of cell.
Five, in the utility model, ware body is provided with spill buffing pad, spill buffing pad is enclosed within the exterior bottom wall of ware body, in cell cultivation process, during mobile ware body, spill buffing pad can play certain shock absorption, prevents nutrient solution large-amplitude sloshing in ware body to affecting cells, is beneficial to the normal cultivation ensureing cell.
Six, in the utility model, ware body and ware lid are clamping or are threaded, and the junction of ware body and ware lid is overlapped and had oversheath, by setting up oversheath, the strength of joint of ware body and ware lid can be strengthened, prevent nutrient solution in transportation from spilling, ensure that cell is immersed in nutrient solution fully.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model;
Fig. 2 is the sectional view of the utility model ware lid;
Fig. 3 is the structural representation of the utility model embodiment 2;
Fig. 4 is the structural representation of the utility model embodiment 3;
Fig. 5 is the structural representation of the utility model embodiment 4;
Fig. 6 is the structural representation of the utility model embodiment 5;
Fig. 7 is the structural representation of the utility model embodiment 6;
Mark in figure: 1, ware body, 2, ware lid, 3, first connecting rod section, 4, spring section, 5, second connecting rod section, 6, transparent catch, 7, connection piece, 8, grip block, 9, cell carries chamber, 10, diversion trench, 11, manhole, 12, sealing plug, 13, spill buffing pad, 14, oversheath, 15, antifogging film, 16, unidirectional tracheae.
Embodiment
Embodiment 1
See Fig. 1 and Fig. 2, a kind of experiment Tissue Culture Dish, the ware lid 2 comprising ware body 1 and be connected on ware body 1, also comprise flexible connecting rod, described flexible connecting rod is syllogic Split type structure, flexible connecting rod is by first connecting rod section 3, spring section 4 and second connecting rod section 5 successively head and the tail are formed by connecting, transparent catch 6 is provided with in described ware body 1, the center of described transparent catch 6 is fixed with connection piece 7, described first connecting rod section 3 is connected with connection piece 7, described second connecting rod section 5 is slidably connected on ware lid 2, described ware lid 2 is fixed with the grip block 8 for clamping second connecting rod section 5, the inwall of described ware lid 2 is bonded with antifogging film 15, the outer wall of described ware lid 2 is connected with unidirectional tracheae 16, described unidirectional tracheae 16 stretches in ware body 1.
The present embodiment is the most basic embodiment, flexible connecting rod is syllogic Split type structure, flexible connecting rod is by first connecting rod section, spring section and second connecting rod section successively head and the tail are formed by connecting, transparent catch is provided with in ware body, the center of transparent catch is fixed with connection piece, first connecting rod section is connected with connection piece, second connecting rod section is slidably connected at ware and covers, ware covers the grip block be fixed with for clamping second connecting rod section, the inwall of ware lid is bonded with antifogging film, the outer wall of ware lid is connected with unidirectional tracheae, unidirectional tracheae stretches in ware body, in cell cultivation process, laboratory technician can at any time directly over culture dish through the change of observation of cell nutrient solution that transparent catch is real-time, take corresponding training measures timely, thus be beneficial to the culture efficiency and culture effect that improve cell, compared to nethike embrane, even if transparent catch contacts with nutrient solution for a long time, be also not easy to soak and damage, can not bacterium be produced, ensure the effect of nutrient solution, improve cell cultures success ratio, by the flexible connecting rod of syllogic and coordinating of grip block, the position of transparent catch in ware body on vertical direction can be adjusted very easily, even if add nutrient solution in cell cultivation process, cell also can be limited in nutrient solution by transparent catch, ensure cell cultures effect, in addition, flexible connecting rod has certain shock absorption, can effectively prevent transparent catch and ware body inwall from producing larger friction and damaging ware body, the inwall of ware lid is bonded with antifogging film, the outer wall of ware lid is connected with unidirectional tracheae, unidirectional tracheae stretches in ware body, in cell cultivation process, when unlatching ware lid adds nutrient solution, air easily enters in ware body, because of the change of envrionment temperature, easily haze in ware body, and antifogging film can prevent from producing in ware body vaporific water droplet, be convenient to the observation of cell, unidirectional tracheae also can discharge the partial air in ware body, the two coordinates, can prevent from completely hazing in ware body, laboratory technician is more clear through ware body observation of cell, greatly improve cell cultures effect.
Embodiment 2
See Fig. 2 and Fig. 3, a kind of experiment Tissue Culture Dish, the ware lid 2 comprising ware body 1 and be connected on ware body 1, also comprise flexible connecting rod, described flexible connecting rod is syllogic Split type structure, flexible connecting rod is by first connecting rod section 3, spring section 4 and second connecting rod section 5 successively head and the tail are formed by connecting, transparent catch 6 is provided with in described ware body 1, the center of described transparent catch 6 is fixed with connection piece 7, described first connecting rod section 3 is connected with connection piece 7, described second connecting rod section 5 is slidably connected on ware lid 2, described ware lid 2 is fixed with the grip block 8 for clamping second connecting rod section 5, the inwall of described ware lid 2 is bonded with antifogging film 15, the outer wall of described ware lid 2 is connected with unidirectional tracheae 16, described unidirectional tracheae 16 stretches in ware body 1.
Be provided with multiple cell for placing cell in described ware body 1 and carry chamber 9, cell carries that chamber 9 is detachable to be connected on the interior diapire of ware body 1, and multiple cell carries chamber 9 and is uniformly distributed in circular.
The present embodiment is a better embodiment, be provided with multiple cell for placing cell in ware body and carry chamber, cell carries on the detachable diapire being connected to ware body in chamber, it is circular being uniformly distributed that multiple cell carries chamber, can cultivate various kinds of cell simultaneously, when certain cell will be observed, this cell of direct taking-up carries chamber, observational study is quite convenient, it is circular being uniformly distributed that multiple cell carries chamber, the situation that each cell carries chamber inner cell can be observed from the sidewall of ware body, can not juxtaposition, thus be more conducive to the cultivation of cell.
Embodiment 3
See Fig. 2 and Fig. 4, a kind of experiment Tissue Culture Dish, the ware lid 2 comprising ware body 1 and be connected on ware body 1, also comprise flexible connecting rod, described flexible connecting rod is syllogic Split type structure, flexible connecting rod is by first connecting rod section 3, spring section 4 and second connecting rod section 5 successively head and the tail are formed by connecting, transparent catch 6 is provided with in described ware body 1, the center of described transparent catch 6 is fixed with connection piece 7, described first connecting rod section 3 is connected with connection piece 7, described second connecting rod section 5 is slidably connected on ware lid 2, described ware lid 2 is fixed with the grip block 8 for clamping second connecting rod section 5, the inwall of described ware lid 2 is bonded with antifogging film 15, the outer wall of described ware lid 2 is connected with unidirectional tracheae 16, described unidirectional tracheae 16 stretches in ware body 1.
Be provided with multiple cell for placing cell in described ware body 1 and carry chamber 9, cell carries that chamber 9 is detachable to be connected on the interior diapire of ware body 1, and multiple cell carries chamber 9 and is uniformly distributed in circular.
Also be provided with diversion trench 10 in described ware body 1, diversion trench 10 carries between chamber 9 at two flanking cells, and diversion trench 10 carries chamber 9 with two cells respectively and is communicated with.
The present embodiment is another better embodiment, also diversion trench is provided with in ware body, diversion trench carries between chamber at two flanking cells, diversion trench carries chamber with two cells respectively and is communicated with, the cell that two flanking cells carry in chamber can be allowed mutually to move by diversion trench, thus be beneficial to the intercellular interaction of research, be convenient to carry out better cell cultures.
Embodiment 4
See Fig. 2 and Fig. 5, a kind of experiment Tissue Culture Dish, the ware lid 2 comprising ware body 1 and be connected on ware body 1, also comprise flexible connecting rod, described flexible connecting rod is syllogic Split type structure, flexible connecting rod is by first connecting rod section 3, spring section 4 and second connecting rod section 5 successively head and the tail are formed by connecting, transparent catch 6 is provided with in described ware body 1, the center of described transparent catch 6 is fixed with connection piece 7, described first connecting rod section 3 is connected with connection piece 7, described second connecting rod section 5 is slidably connected on ware lid 2, described ware lid 2 is fixed with the grip block 8 for clamping second connecting rod section 5, the inwall of described ware lid 2 is bonded with antifogging film 15, the outer wall of described ware lid 2 is connected with unidirectional tracheae 16, described unidirectional tracheae 16 stretches in ware body 1.
Be provided with multiple cell for placing cell in described ware body 1 and carry chamber 9, cell carries that chamber 9 is detachable to be connected on the interior diapire of ware body 1, and multiple cell carries chamber 9 and is uniformly distributed in circular.
Also be provided with diversion trench 10 in described ware body 1, diversion trench 10 carries between chamber 9 at two flanking cells, and diversion trench 10 carries chamber 9 with two cells respectively and is communicated with.
Described ware lid 2 has multiple manhole 11, and multiple manhole 11 is evenly distributed on ware lid 2 in the form of a ring, and it is corresponding that manhole 11 and cell carry chamber 9, manhole 11 is embedded with sealing plug 12.
The present embodiment is another better embodiment, ware covers and has multiple manhole, multiple manhole is evenly distributed on ware in the form of a ring and covers, it is corresponding that manhole and cell carry chamber, manhole is embedded with sealing plug, in cell cultivation process, when a certain cell will be taken out to be observed, only need open the sealing plug on respective circular through hole, just can take out cell and carry chamber, operation is quite simple, can effectively prevent nutrient solution big area from exposing in atmosphere, ensure the effect of nutrient solution, thus improve the cultivation rate of cell.
Embodiment 5
See Fig. 2 and Fig. 6, a kind of experiment Tissue Culture Dish, the ware lid 2 comprising ware body 1 and be connected on ware body 1, also comprise flexible connecting rod, described flexible connecting rod is syllogic Split type structure, flexible connecting rod is by first connecting rod section 3, spring section 4 and second connecting rod section 5 successively head and the tail are formed by connecting, transparent catch 6 is provided with in described ware body 1, the center of described transparent catch 6 is fixed with connection piece 7, described first connecting rod section 3 is connected with connection piece 7, described second connecting rod section 5 is slidably connected on ware lid 2, described ware lid 2 is fixed with the grip block 8 for clamping second connecting rod section 5, the inwall of described ware lid 2 is bonded with antifogging film 15, the outer wall of described ware lid 2 is connected with unidirectional tracheae 16, described unidirectional tracheae 16 stretches in ware body 1.
Be provided with multiple cell for placing cell in described ware body 1 and carry chamber 9, cell carries that chamber 9 is detachable to be connected on the interior diapire of ware body 1, and multiple cell carries chamber 9 and is uniformly distributed in circular.
Also be provided with diversion trench 10 in described ware body 1, diversion trench 10 carries between chamber 9 at two flanking cells, and diversion trench 10 carries chamber 9 with two cells respectively and is communicated with.
Described ware lid 2 has multiple manhole 11, and multiple manhole 11 is evenly distributed on ware lid 2 in the form of a ring, and it is corresponding that manhole 11 and cell carry chamber 9, manhole 11 is embedded with sealing plug 12.
Described ware body 1 is provided with spill buffing pad 13, and spill buffing pad 13 is enclosed within the exterior bottom wall of ware body 1.
The present embodiment is another better embodiment, ware body is provided with spill buffing pad, spill buffing pad is enclosed within the exterior bottom wall of ware body, in cell cultivation process, during mobile ware body, spill buffing pad can play certain shock absorption, prevents nutrient solution large-amplitude sloshing in ware body to affecting cells, is beneficial to the normal cultivation ensureing cell.
Embodiment 6
See Fig. 2 and Fig. 7, a kind of experiment Tissue Culture Dish, the ware lid 2 comprising ware body 1 and be connected on ware body 1, also comprise flexible connecting rod, described flexible connecting rod is syllogic Split type structure, flexible connecting rod is by first connecting rod section 3, spring section 4 and second connecting rod section 5 successively head and the tail are formed by connecting, transparent catch 6 is provided with in described ware body 1, the center of described transparent catch 6 is fixed with connection piece 7, described first connecting rod section 3 is connected with connection piece 7, described second connecting rod section 5 is slidably connected on ware lid 2, described ware lid 2 is fixed with the grip block 8 for clamping second connecting rod section 5, the inwall of described ware lid 2 is bonded with antifogging film 15, the outer wall of described ware lid 2 is connected with unidirectional tracheae 16, described unidirectional tracheae 16 stretches in ware body 1.
Be provided with multiple cell for placing cell in described ware body 1 and carry chamber 9, cell carries that chamber 9 is detachable to be connected on the interior diapire of ware body 1, and multiple cell carries chamber 9 and is uniformly distributed in circular.
Also be provided with diversion trench 10 in described ware body 1, diversion trench 10 carries between chamber 9 at two flanking cells, and diversion trench 10 carries chamber 9 with two cells respectively and is communicated with.
Described ware lid 2 has multiple manhole 11, and multiple manhole 11 is evenly distributed on ware lid 2 in the form of a ring, and it is corresponding that manhole 11 and cell carry chamber 9, manhole 11 is embedded with sealing plug 12.
Described ware body 1 is provided with spill buffing pad 13, and spill buffing pad 13 is enclosed within the exterior bottom wall of ware body 1.
Described ware body 1 and ware lid 2 are for clamping or be threaded, and ware body 1 overlaps with the junction of ware lid 2 oversheath 14.
The present embodiment is preferred forms, flexible connecting rod is syllogic Split type structure, flexible connecting rod is by first connecting rod section, spring section and second connecting rod section successively head and the tail are formed by connecting, transparent catch is provided with in ware body, the center of transparent catch is fixed with connection piece, first connecting rod section is connected with connection piece, second connecting rod section is slidably connected at ware and covers, ware covers the grip block be fixed with for clamping second connecting rod section, the inwall of ware lid is bonded with antifogging film, the outer wall of ware lid is connected with unidirectional tracheae, unidirectional tracheae stretches in ware body, in cell cultivation process, laboratory technician can at any time directly over culture dish through the change of observation of cell nutrient solution that transparent catch is real-time, take corresponding training measures timely, thus be beneficial to the culture efficiency and culture effect that improve cell, compared to nethike embrane, even if transparent catch contacts with nutrient solution for a long time, be also not easy to soak and damage, can not bacterium be produced, ensure the effect of nutrient solution, improve cell cultures success ratio, by the flexible connecting rod of syllogic and coordinating of grip block, the position of transparent catch in ware body on vertical direction can be adjusted very easily, even if add nutrient solution in cell cultivation process, cell also can be limited in nutrient solution by transparent catch, ensure cell cultures effect, in addition, flexible connecting rod has certain shock absorption, can effectively prevent transparent catch and ware body inwall from producing larger friction and damaging ware body, the inwall of ware lid is bonded with antifogging film, the outer wall of ware lid is connected with unidirectional tracheae, unidirectional tracheae stretches in ware body, in cell cultivation process, when unlatching ware lid adds nutrient solution, air easily enters in ware body, because of the change of envrionment temperature, easily haze in ware body, and antifogging film can prevent from producing in ware body vaporific water droplet, be convenient to the observation of cell, unidirectional tracheae also can discharge the partial air in ware body, the two coordinates, can prevent from completely hazing in ware body, laboratory technician is more clear through ware body observation of cell, greatly improve cell cultures effect.Ware body and ware lid are clamping or are threaded, and the junction of ware body and ware lid is overlapped and had oversheath, by setting up oversheath, can strengthen the strength of joint of ware body and ware lid, prevent nutrient solution in transportation from spilling, and ensure that cell is immersed in nutrient solution fully.
Claims (6)
1. an experiment Tissue Culture Dish, comprise ware body (1) and be connected to the ware lid (2) on ware body (1), it is characterized in that: also comprise flexible connecting rod, described flexible connecting rod is syllogic Split type structure, flexible connecting rod is by first connecting rod section (3), spring section (4) and second connecting rod section (5) successively head and the tail are formed by connecting, transparent catch (6) is provided with in described ware body (1), the center of described transparent catch (6) is fixed with connection piece (7), described first connecting rod section (3) is connected with connection piece (7), described second connecting rod section (5) is slidably connected on ware lid (2), described ware lid (2) is fixed with the grip block (8) for clamping second connecting rod section (5), the inwall of described ware lid (2) is bonded with antifogging film (15), the outer wall of described ware lid (2) is connected with unidirectional tracheae (16), described unidirectional tracheae (16) is stretched in ware body (1).
2. a kind of experiment Tissue Culture Dish according to claim 1, it is characterized in that: be provided with multiple cell for placing cell in described ware body (1) and carry chamber (9), cell carries on the detachable interior diapire being connected to ware body (1) in chamber (9), and multiple cell carries chamber (9) and is uniformly distributed in circular.
3. a kind of experiment Tissue Culture Dish according to claim 2, it is characterized in that: in described ware body (1), be also provided with diversion trench (10), diversion trench (10) is positioned at two flanking cells and carries between chamber (9), and diversion trench (10) carries chamber (9) with two cells respectively and is communicated with.
4. a kind of experiment Tissue Culture Dish according to claim 3, it is characterized in that: described ware lid (2) has multiple manhole (11), multiple manhole (11) is evenly distributed on ware lid (2) in the form of a ring, it is corresponding that manhole (11) and cell carry chamber (9), manhole (11) is embedded with sealing plug (12).
5. a kind of experiment Tissue Culture Dish according to claim 4, is characterized in that: described ware body (1) is provided with spill buffing pad (13), and spill buffing pad (13) is enclosed within the exterior bottom wall of ware body (1).
6. a kind of experiment Tissue Culture Dish according to claim 1 or 5, it is characterized in that: described ware body (1) and ware lid (2) are for clamping or be threaded, the junction of ware body (1) and ware lid (2) is overlapped and is had oversheath (14).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520773669.4U CN205035388U (en) | 2015-10-08 | 2015-10-08 | Cell culture dish for experiments |
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| CN201520773669.4U CN205035388U (en) | 2015-10-08 | 2015-10-08 | Cell culture dish for experiments |
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| CN205035388U true CN205035388U (en) | 2016-02-17 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111363681A (en) * | 2020-03-19 | 2020-07-03 | 江苏省人民医院（南京医科大学第一附属医院） | Integrated primary cell climbing culture bottle |
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2015
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111363681A (en) * | 2020-03-19 | 2020-07-03 | 江苏省人民医院（南京医科大学第一附属医院） | Integrated primary cell climbing culture bottle |
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