CN103620018A - Cell cultivator - Google Patents
Cell cultivator Download PDFInfo
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- CN103620018A CN103620018A CN201180071316.4A CN201180071316A CN103620018A CN 103620018 A CN103620018 A CN 103620018A CN 201180071316 A CN201180071316 A CN 201180071316A CN 103620018 A CN103620018 A CN 103620018A
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- ring
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- cell body
- cell
- division
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/02—Membranes; Filters
- C12M25/04—Membranes; Filters in combination with well or multiwell plates, i.e. culture inserts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Provided is a cell cultivator, said cell cultivator includes a hollow tank, a removable membrane, a fixed sheath and a support seat, wherein the removable membrane is fixed on the bottom opening of the tank, the support seat is located on the outside of the tank and supports the tank, living cells or others biological materials can be placed on the membrane to culture. The position of the tank of said cell cultivator, relative to the support seat, can be adjusted to facilitate the microscope focusing. After the cell culture is ending, the fixed sheath can be removed from the bottom of the tank in order to remove the membrane to observe it or to use it in clinical and medical applications.
Description
Cell culture apparatus technical field
The present invention is on a kind of thin born of the same parents' incubator, espespecially one kind can be applied to cell culture utensil used in active somatic cell culture field, or as carrier used in biomaterial microexamination field, in order to active somatic cell or the cell culture apparatus design of the progress microexamination of other biological material and shooting record.And this cell culture apparatus can also be applied to by its indirect or directly transplanting human body or animal body after culture active somatic cell, or biomedical material is further combined in any form in the cell training medium application field of device.Background technology
In the research and application correlative technology field of such as cell biology, for carry out the culture of cell in vitro or be tissue shaping, cell line set up, the test of cell physiological toxicity, cell migration observation, the various researchs between cell and another cell or other extracellular matrixs or the factor and application, or when experiment made on the living is difficult to walk, generally cell culture apparatus must be used to provide stem cell and the purposes of other all kinds cell injuring models.Above-mentioned various cells can be combined further with any type of biomedical material, and applied to medical research and graft application.
Existing cell culture apparatus design at present, it mainly includes the accommodation space that a up/down perforation shape is formed in a cell body and a diaphragm with three support feets, described cell body, and the diaphragm is fixed at cell body bottom opening.It is to provide active somatic cell to be fixed on the diaphragm of cell body bottom to adhere to its surface when the cell culture apparatus is applied to cell culture, and soaks and be placed in culture medium, active somatic cell is survived growth.
In foregoing cell culture apparatus, cell body is to manufacture to shape with forging type by mold mostly, though cell body takes the tectonic sieving of foot-rest type, culture can be provided based on circulation good on diaphragm, grown up with sharp active somatic cell, but, because the cell body manufactured using moulding is fixed for a shape, immutable type body, without adjustable, and diaphragm is fixed on cell body bottom can not arbitrarily tear group open, when being applied in clinical treatment, or when user's living cells observation to be carried out or filming image, micro-imaging observation must be carried out together with cell body, when wherein because using high power objective, its high power camera lens NA values are big, operating distance is short, when lens focusing is adjusted, easily because cell body is located at the obstruction above diaphragm, and unfavorable microscope obtains more visible image to diaphragm.The content of the invention
The technical problem to be solved in the present invention is：The diaphragm for improving existing cell culture apparatus at present is fixed on the shortcoming that cell body bottom is not easy to disassemble.
To solve foregoing technical problem, technical solution of the invention is：A kind of cell culture apparatus is provided, it is included：
A upper shed is formed at the top of the cell body of one hollow form, cell body, an opening and one is formed positioned at the location division of the mouth periphery in cell body bottom；
One diaphragm, is removable group and is covered at the opening of cell body bottom, and diaphragm periphery extends to the location division side of cell body bottom；
One fixed cover, is removable group and is installed on the location division of cell body bottom, diaphragm is fixed on into cell body bottom；And
One support base, is to include a pedestal, pedestal is mounted on the outside of cell body and supports cell body.
In cell culture apparatus as described above, the pedestal of the support base has an annular solid,；F annular base body root edges extend downwardly several support feets, and the pedestal can be positioningly mounted on the outside of cell body in adjustment up and down.
In cell culture apparatus as described above, the pedestal top forms the inner ring convex portion diametrically extended inwardly, inner ring periphery formation in inner ring convex portion is spaced constituted adjustment location division by multiple convex grains, outer peripheral face of the cell body above location division forms several spaced positioning concave edge up and down, there is provided the positioning of adjustment location division clip wherein, enable cell body lift adjustment height.
In cell culture apparatus as described above, the pedestal inner ring convex portion bottom, which is formed, can push against the pressure section of fixed cover.
In cell culture apparatus as described above, the location division is formed from the ring-type Access Division in cell body bottom periphery face, the fixed cover has a ring-type body for being sheathed on cell body periphery, side face formation ring-type Fixed Division in the annular casing body, ring-type Access Division and ring-type Fixed Division are the male-female engagements to form arc surface, it is arranged fixed cover and is fixed on cell body periphery, diaphragm is gripped between ring-type Access Division and ring-type Fixed Division.
In cell culture apparatus as described above, shape Access Division is not the arc surface flange of ring-type, and ring-type Fixed Division is the arc surface concave edge of ring-type.
In cell culture apparatus as described above, ring-type Access Division is the arc surface concave edge of ring-type, and ring-type Fixed Division is the arc surface flange of ring-type. .
In cell culture apparatus as described above, the ring-type body bottom of the fixed cover forms the ring root edge extended inwardly, and the ring root edge of fixed cover is relative with the location division of cell body bottom to clamp diaphragm.
In cell culture apparatus as described above, ring-type Access Division is the arc surface flange of ring-type, and ring-type Fixed Division is the arc surface concave edge of ring-type；The ring-type body bottom of the fixed cover forms the ring root edge extended inwardly, and the ring root edge of fixed cover is relative with the location division of cell body bottom to clamp diaphragm.
In cell culture apparatus as described above, ring-type Access Division is the arc surface concave edge of ring-type, ring-type Fixed Division
For the arc surface flange of ring-type;The ring-type body bottom of the fixed cover forms the ring root edge extended inwardly, and the ring root edge of fixed cover is relative with the location division of cell body bottom to clamp diaphragm.
It is an advantage of the invention that：In cell cultivation process, user removes the appropriate height and position being placed in using the diaphragm leaching located at cell body bottom surface in the culture medium of incubator, allow the nutrient solution that culture medium included that there is good permeability in diaphragm with additive, make membrane surface active somatic cell can be by good culture outside；When user is intended to carry out cell observation by microscope, annular base body is up adjusted, cell body bottom is approached or is placed on microscopical objective table, allow user accurately to adjust microscope focusing, to obtain comparatively ideal micro-imaging.Come to an end in cell culture, also fixed cover can be pulled down from cell body bottom surface, carry out cell or the transplanting of celliferous biomedical material, or taking-up diaphragm is placed under microscopical object lens and directly observed, so, cell body and its support base of periphery can be avoided to hinder microscopical, allow user accurately to adjust microscope focusing, and obtain comparatively ideal ^ born of the same parents' image.
In the cell culture apparatus of the present invention, still there is an annular solid using the pedestal of the support base, several support feets are extended downwardly in annular base body root edge, the pedestal can be positioningly mounted on the outside of cell body in adjustment up and down, and the diaphragm for being arranged at cell body bottom with profit is easy to adjust its height and position up and down.Brief description of the drawings
Fig. 1 is the perspective exploded view of the first preferred embodiment of cell culture apparatus of the present invention.
Fig. 2 is the combined planar schematic diagram of the preferred embodiment of cell culture apparatus first shown in Fig. 1.
Fig. 3 is the close-up schematic view of the preferred embodiment of cell culture apparatus first shown in Fig. 2.
Fig. 4 is the combined planar schematic diagram for another preferred embodiment that the ring-type Access Division of cell culture apparatus preferred embodiment shown in Fig. 1 is exchanged with the concaveconvex shape of ring-type Fixed Division.
Fig. 5 is the combined planar schematic diagram of the preferred embodiment of cell culture apparatus second of the present invention.
Fig. 6 is the close-up schematic view of the preferred embodiment of cell culture apparatus second shown in Fig. 5.
Fig. 7 is that the support base in the preferred embodiment of cell culture apparatus first shown in Fig. 1 rises and reduces the floor map of cell culture apparatus height.
Fig. 8 is the operation chart that the preferred embodiment of cell culture apparatus first shown in Fig. 1 is disassembled.
Description of reference numerals
1 0- cell bodies；1 1- is open；12- location divisions；13,1 3a- ring-types Access Division；14- positions concave edge；1 5- rings top flange；20- diaphragms；30- fixed covers；300- ring-type bodies；301-ring root edge；31st, 31 a- ring-types Fixed Division；40- support bases；41- pedestals；42- support feets；4 3- inner ring convex portions；44- adjusts location division；The convex grains of 441-；45- pressure sections.
Embodiment
Coordinate schema and presently preferred embodiments of the present invention below, the present invention is expanded on further to reach the technological means that predetermined goal of the invention is taken.
As shown in Figure 1 to Figure 4 with shown in Fig. 5 to Fig. 6, be the several preferred embodiments for disclosing cell culture apparatus of the present invention, can see in those schemas and, the cell culture apparatus designed by the present invention is mainly and included：One cell body 10, a diaphragm 20, a fixed cover 30 and a support base 40, wherein：
The cell body 10 is to form a upper shed at the top of the hollow body of a up/down perforation shape, cell body 10, and forming an opening 11 and one in the bottom of cell body 10 is located at the peripheral location division 12 of opening 11.
The diaphragm 20 is the porous diaphragm with permeability, and the diaphragm 20 is removable group and is covered at the opening of the bottom of cell body 10, and the periphery of diaphragm 20 extends to the side of location division 12 of the bottom of cell body 10.
The fixed cover 30 is removable group and is installed on the location division 12 of the bottom of cell body 10, diaphragm 20 is entirely fixed on into the bottom of cell body 10.
The support base 40 includes a pedestal 41, or further comprising several support feets 42, the several support feets are extended downwardly located at the bottom of pedestal 41, and the support base 40 is to be mounted on the outside of cell body 10 with the pedestal 41, to support cell body 10.
As shown in Fig. 1 to Fig. 3 and Fig. 5 to Fig. 6, the location division 12 of the cell body 10 is formed from the ring-type Access Division 13 in the bottom periphery face of cell body 10;First preferred embodiment as shown in Figure 1 to Figure 3, the fixed cover 30 has one can be sheathed on the ring-type body 300 of the periphery of cell body 10, the ring root edge 301 extended inwardly is formed in the bottom of ring-type body 300, or, second preferred embodiment as shown in Figure 5 to Figure 6, the fixed cover 30 has one can be sheathed on the ring-type body 300 of the periphery of cell body 10;The fixed cover 30 is in the inner peripheral surface of ring-type body 300 formation ring-type Fixed Division 31, ring-type Access Division 13 is that the male-female engagement to form arc surface is constructed with ring-type Fixed Division 31, in the first preferred embodiment shown in Fig. 2 or Fig. 3, ring-type Access Division 13 is the arc surface flange of ring-type, then designed for the arc surface concave edge of ring-type ring-type Fixed Division 31, in another preferred embodiment shown in Fig. 4, ring-type Access Division 13a is alternatively the arc surface concave edge of ring-type, ring-type Fixed Division 31a then designs for the arc surface flange of ring-type, so, it can be arranged the ring-type body 300 of fixed cover 30 and be fixed on the periphery of cell body 10, diaphragm 20 is gripped between ring-type Access Division 13 and ring-type Fixed Division 31, and utilize ring-type Access Division 13 and male-female engagement of the ring-type Fixed Division 31 for arc surface, make that not only there is linking steady character between the two, and with the function for being easy to combine and disassembling；If the fixed cover 30 is when the bottom of ring-type body 300 has ring root edge 301, ring root edge 301 then further relative with the location division 12 of the bottom of cell body 10 can clamp diaphragm 20.
The pedestal 41 of the support base 40 is an annular solid, and the several support feets 42 form in the root edge of annular base body 41 and extend downwardly, and the pedestal 41 simultaneously can be positioningly mounted on the outside of cell body 10 in adjustment up and down, such as Fig. 2 or
In preferred embodiment shown in Fig. 3, the top of pedestal 41 forms the inner ring convex portion 43 diametrically extended inwardly, in the inner ring periphery of inner ring convex portion 43 formation adjustment location division 44, the inner ring periphery that the adjustment location division 44 can be spaced in inner ring convex portion 43 by multiple convex grains 441 is constituted, outer peripheral face of the cell body 10 in the top of location division 12 forms the positioning concave edge 14 of spaced ring-type above and below several, to provide the positioning of the adjustment clip of location division 44 wherein, make cell body 10 can relative support seat about 40 adjustment highly, position positions concave edge 14 close to the apical margin of cell body 10 upper one in several ring-type positioning concave edge 14；In the preferred embodiment shown in Fig. 1 to Fig. 3, support base 40 also can form pressure section 45 in the bottom surface of inner ring convex portion 43, be used as the position for pushing pushing fixed cover 30.
In preferred embodiment as shown in Figure 1 or 2, the apical margin of cell body 10 one ring top flange 15 of formation, the external diameter of ring top flange 15 is more than the internal diameter of inner ring convex portion 43 of support base 40, cell body 10 is set from top to bottom to pass through in support base 40, and can be spacing to the only inner ring convex portion 43 of support base 40 by ring top flange 15.
The cell culture apparatus of the present invention in use, cell culture apparatus leaching be placed in include nutrient solution and additive ... culture medium in, separately by active somatic cell to be cultivated（Or the combination of cell and biomedical material) the appropriate and smooth surface of porous diaphragm 20 of permeability is attached to, active somatic cell is continued survival culture in the medium.
As shown in Figure 7, in incubation, user can adjust the high and low position of cell body 10 using support base 40, the diaphragm 20 of the bottom surface of cell body 10 is set to be located at appropriate height and position in culture medium, allow the nutrient solution included in culture medium that there is good permeability in diaphragm 20 with additive, make the active somatic cell on the surface of diaphragm 20 can be by good culture.
When user is intended to carry out cell observation by microscope, annular base body 41 is up adjusted, makes cell body
The diaphragm 20 of 10 bottoms can be approached or be placed on microscopical objective table, allow user accurately to adjust microscope focusing, to obtain comparatively ideal micro-imaging.Come to an end in cell culture, as shown in Fig. 8, fixed cover 30 can be pulled down from the bottom surface of cell body 10, wherein pushing fixed cover 30 is pushed using the pressure section 45 of support base 40, and constructed using the curved planar male-female engagement of ring-type Access Division 13 and ring-type Fixed Division 31, fixed cover 30 is set to depart from cell body 10, take out diaphragm 20, the diaphragm 20 being removed can be directly used for clinical cytology transplanting or the transplanting of celliferous biomedical material, or be placed under microscopical object lens and directly observe, so, cell body 10 and its support base 40 of periphery can be avoided to hinder microscopical, the user can accurately adjust microscope focusing, and obtain comparatively ideal cell image.
In summary, the present invention cell culture apparatus can really solve cell observation time slot body periphery support base highly make microscope can not exact focus the problem of, and the cell culture apparatus designed by the present invention is easy to the disassembling operations of diaphragm, therefore in the technical field such as technical field of cell culture or other biological material and regenerative medicine, really
The practical cell culture apparatus of one great industrial utilization can be provided.
The above description of this invention is illustrative; and it is nonrestrictive; those skilled in the art understands, can carry out many modifications, change or equivalent to it within the spirit and scope that claim is limited, but they are fallen within protection scope of the present invention.
Claims (9)
- Claim1. a kind of cell culture apparatus, it is characterised in that：It includes：A upper shed is formed at the top of the cell body of one hollow form, cell body, an opening and one is formed positioned at the location division of the mouth periphery in cell body bottom；One diaphragm, be can tear open group be covered at the opening of cell body bottom, and diaphragm periphery extend to cell body bottom location division side；One fixed cover, be can tear open group be installed on the location division of cell body bottom, the diaphragm is fixed on cell body bottom；AndOne support base, is to include a pedestal, the pedestal is mounted on the outside of the cell body and supports cell body.2. cell culture apparatus according to claim 1, it is characterised in that：The pedestal of the support base has an annular solid, and several support feets are extended downwardly in annular base body root edge, and the pedestal can be positioningly mounted on the outside of cell body in adjustment up and down.3. cell culture apparatus according to claim 2, it is characterised in that：The pedestal top forms the inner ring convex portion diametrically extended inwardly, inner ring periphery formation in inner ring convex portion is spaced constituted adjustment location division by multiple convex grains, outer peripheral face of the cell body above location division forms several spaced positioning concave edge up and down, there is provided the positioning of adjustment location division clip wherein, enable cell body lift adjustment height.4. cell culture apparatus according to claim 3, it is characterised in that：This inner ring convex portion bottom, which is formed, can push against the pressure section of fixed cover.5. the cell culture apparatus according to any one of Claims 1-4, its feature exists：The location division is formed from the ring-type Access Division in cell body bottom periphery face, the fixed cover has a ring-type body for being sheathed on cell body periphery, side face formation ring-type Fixed Division in the annular casing body, ring-type Access Division and ring-type Fixed Division are the male-female engagements to form arc surface, it is arranged fixed cover and is fixed on cell body periphery, the diaphragm is gripped between ring-type Access Division and ring-type Fixed Division.6. cell culture apparatus according to claim 5, it is characterised in that：Ring-type Access Division is the arc surface flange of ring-type, and ring-type Fixed Division is the arc surface concave edge of ring-type.7. cell culture apparatus according to claim 5, it is characterised in that：Ring Access Division is the arc surface concave edge of ring-type, and ring-type Fixed Division is the arc surface flange of ring-type..8. cell culture apparatus according to claim 5, it is characterised in that：The ring-type body bottom of the fixed cover forms the ring root edge extended inwardly, and the ring root edge of fixed cover is relative with the location division of cell body bottom to clamp diaphragm.9. cell culture apparatus according to claim 5, it is characterised in that：Ring-type Access Division is ring-type Arc surface flange, ring-type Fixed Division is the arc surface concave edge of ring-type；The ring-type body bottom of the fixed cover forms the ring root edge extended inwardly, and the ring root edge of fixed cover is relative with the location division of cell body bottom to clamp diaphragm.1 0. cell culture apparatuses according to claim 5, it is characterised in that：Ring-type Access Division is the arc surface concave edge of ring-type, and ring-type Fixed Division is the arc surface flange of ring-type；The ring-type body bottom of the fixed cover forms the ring root edge extended inwardly, and the ring root edge of fixed cover is relative with the location division of cell body bottom to clamp diaphragm.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2011/000924 WO2012162855A1 (en) | 2011-06-01 | 2011-06-01 | Cell cultivator |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103620018A true CN103620018A (en) | 2014-03-05 |
| CN103620018B CN103620018B (en) | 2015-05-20 |
Family
ID=47258258
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201180071316.4A Active CN103620018B (en) | 2011-06-01 | 2011-06-01 | Cell cultivator |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN103620018B (en) |
| WO (1) | WO2012162855A1 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ITMI20131494A1 (en) * | 2013-09-10 | 2015-03-11 | Univ Pisa | INNOVATIVE SUPPORT FOR CELL CULTURE |
| WO2020001733A1 (en) * | 2018-06-25 | 2020-01-02 | Universitätsklnikum Hamburg-Eppendorf | A holding device for microscopy applications and microscopy chamber |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6057150A (en) * | 1997-09-19 | 2000-05-02 | Bio-Rad Laboratories, Inc. | Biaxial strain system for cultured cells |
| US20080076170A1 (en) * | 2006-09-27 | 2008-03-27 | Tuija Annala | Cell culture insert and cell culture vessel |
| TW200944589A (en) * | 2008-04-18 | 2009-11-01 | Lynn Ling-Hui Huang | Embedded culture tank |
| WO2010045753A1 (en) * | 2008-10-22 | 2010-04-29 | Huang Lynn L H | Flushtype culture tank |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3332991A1 (en) * | 1982-09-13 | 1984-03-15 | Konishiroku Photo Industry Co., Ltd., Tokyo | METHOD FOR FORMING A COLOR DIFFUSION TRANSFER IMAGE BY WARM DEVELOPING |
| US5026649A (en) * | 1986-03-20 | 1991-06-25 | Costar Corporation | Apparatus for growing tissue cultures in vitro |
| EP0590485B1 (en) * | 1992-09-28 | 1998-07-29 | Becton, Dickinson and Company | Cell culture insert |
| CN201195726Y (en) * | 2008-02-19 | 2009-02-18 | 中国航天员科研训练中心 | Combined cell culture bottle |
-
2011
- 2011-06-01 WO PCT/CN2011/000924 patent/WO2012162855A1/en active Application Filing
- 2011-06-01 CN CN201180071316.4A patent/CN103620018B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6057150A (en) * | 1997-09-19 | 2000-05-02 | Bio-Rad Laboratories, Inc. | Biaxial strain system for cultured cells |
| US20080076170A1 (en) * | 2006-09-27 | 2008-03-27 | Tuija Annala | Cell culture insert and cell culture vessel |
| TW200944589A (en) * | 2008-04-18 | 2009-11-01 | Lynn Ling-Hui Huang | Embedded culture tank |
| WO2010045753A1 (en) * | 2008-10-22 | 2010-04-29 | Huang Lynn L H | Flushtype culture tank |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103620018B (en) | 2015-05-20 |
| WO2012162855A1 (en) | 2012-12-06 |
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