CN206940910U

CN206940910U - It is conveniently replaceable the T cell culture dish of culture medium

Info

Publication number: CN206940910U
Application number: CN201720731977.XU
Authority: CN
Inventors: 张巍
Original assignee: Chongqing Precision Biological Industry Technology Research Institute Co Ltd
Current assignee: Chongqing Precision Biological Industry Technology Research Institute Co Ltd
Priority date: 2017-06-19
Filing date: 2017-06-19
Publication date: 2018-01-30
Anticipated expiration: 2027-06-19

Abstract

The utility model discloses a kind of T cell culture dish for being conveniently replaceable culture medium, including interior ware and the outer ware outside interior ware is covered, the mouth rim of the interior ware is placed on the oral area end face of outer ware, and interior ware oral area is additionally provided with ware lid；The bottom of the interior ware is provided with culture medium via and covers the cell filtering net on culture medium via.For this T cell culture dish when changing culture medium, without opening ware lid, the replacing of culture medium is simple and convenient, and can avoid polluting cell.

Description

It is conveniently replaceable the T cell culture dish of culture medium

Technical field

Technical field of cell culture is the utility model is related to, more particularly to a kind of T cell culture dish.

Background technology

T cell is segmented into many types according to function and surface marker：

1. cytotoxic T cell (cytotoxic T cell)：The function of infected cell these cells is eliminated just as one Individual " killer " or cytotoxin are such, because they can be killed to producing special antigen reactive target cell.Cell The major surfaces mark of malicious T cell is CD8.

2. helper cell (helper T cell) plays the part of the role of pilot process in immune response：It can be expanded with hyperplasia Dissipate to activate the immunocyte of other types of generation direct immunization reaction.The major surfaces mark of helper cell is CD4.T is thin Born of the same parents regulate and control or the performance function of " auxiliary " other lymphocytes.They are the target cells of known inhibition of HIV, are fallen ill in AIDS When can drastically reduce.

3. regulation/suppression T cell (regulatory/suppressor T cell)：Negative regulator organism immune response.It is logical Often play the important function for maintaining self tolerance and avoiding immune response excess damage body.Regulation/suppression T cell has a lot Kind, it is CD25+CD4+T cells to study at present most active.

With the development of biological therapy clinical practice, T cell is more and more extensive in the research and application of medical field.Make It is used for clinical treatment with T cell, it is necessary first to expand substantial amounts of T cell in vitro.In the prior art, for cultivating T cell Culture dish is generally vessel with cover., it is necessary to which often opening vessel cover carries out the replacing of culture medium in incubation, and uncap Change culture medium and easily cause pollution, while the replacing convenience of culture medium is also poor.

Utility model content

In view of this, the purpose of this utility model is to provide a kind of T cell culture dish for being conveniently replaceable culture medium, to solve Existing culture dish need to open lid could more preferable culture medium, culture medium changes convenience difference and easily causes the technologies such as pollution to be asked Topic.

The utility model is conveniently replaceable the T cell culture dish of culture medium, including interior ware and covers the outer ware outside interior ware, institute The mouth rim for stating interior ware is placed on the oral area end face of outer ware, and interior ware oral area is additionally provided with ware lid；The bottom of the interior ware It is provided with culture medium via and covers the cell filtering net on culture medium via.

Further, culture medium flowing lumen, the side of the outer ware are provided between the bottom of the interior ware and the bottom of outer ware Be provided with the discharging tube that enters connected with culture medium flowing lumen on face, it is described enter discharging tube oral area be provided with seal plug.

The beneficial effects of the utility model：

1st, the utility model is conveniently replaceable the T cell culture dish of culture medium, and its interior ware bottom is provided with cell filtering net, more When changing culture medium, interior ware is lifted, the culture medium in interior ware can flow into outer ware by cell filtering net, by the old training in outer ware Foster base is outwelled, and adds new culture medium, then interior ware is put into outer ware, and the new culture medium in outer ware can be entered by cell filtering net Enter interior ware, do not have to open ware lid in Renewal process, so as to realize the replacing of culture medium, the replacing of culture medium is simple and convenient, and can Avoid polluting cell.

2nd, the utility model is conveniently replaceable the T cell culture dish of culture medium, by being set in outer ware side into discharging tube, makes Obtain when changing culture medium, suction pipe or syringe can be extended into discharging tube, old culture medium is extracted out, then again by new training Support base and added from discharging tube is entered, so that the simpler convenience of the replacing of culture medium, interior ware and ware lid has not had to out, can be more preferable The pollution problem for avoiding cell quilt.

Brief description of the drawings

Fig. 1 is the plan structure signal for the T cell culture dish that the utility model is conveniently replaceable culture medium；

Fig. 2 is along A-A sectional view in Fig. 1；

Fig. 3 is the overlooking the structure diagram of outer ware.

Embodiment

The utility model is further described with reference to the accompanying drawings and examples.

As illustrated, the present embodiment is conveniently replaceable the T cell culture dish of culture medium, including interior ware 1 and cover outside interior ware Outer ware 2, the mouth rim 3 of the interior ware is placed on the oral area end face of outer ware, and interior ware oral area is additionally provided with ware lid 4；Institute The bottom for stating interior ware is provided with culture medium via 5 and the cell filtering net 6 covered on culture medium via.

The present embodiment is conveniently replaceable the T cell culture dish of culture medium, and its interior ware bottom is provided with cell filtering net, changes training When supporting base, interior ware is lifted, the culture medium in interior ware can flow into outer ware by cell filtering net, by the old culture medium in outer ware Outwell, add new culture medium, then interior ware is put into outer ware, in the new culture medium in outer ware can be entered by cell filtering net Ware, so as to realize the replacing of culture medium, the replacing of culture medium is simple and convenient, and does not have to open ware lid in Renewal process, can avoid Cell is polluted.

As the improvement to the present embodiment, culture medium flowing lumen is provided between the bottom of the interior ware and the bottom of outer ware 7, be provided with the side of the outer ware connected with culture medium flowing lumen enter discharging tube 8, it is described enter discharging tube oral area set There is seal plug 9.

This improvement in outer ware side by setting into discharging tube so that, can be by suction pipe or syringe when changing culture medium Discharging tube is extended into, old culture medium is extracted out, then again adds new culture medium from discharging tube is entered, so that culture medium Simpler convenience is changed, interior ware and ware lid has not had to out, can preferably avoid the pollution problem of cell quilt.

As the improvement to the present embodiment, the bottom of the outer ware is provided with the multiblock technique 10 with number designation, originally changes Enter so that T cell culture dish is placed on when being observed under inverted microscope, cell can accurately be found according to multiblock technique, be easy to weight Multiple observation.

Finally illustrate, above example is only unrestricted to illustrate the technical solution of the utility model, although ginseng The utility model is described in detail according to preferred embodiment, it will be understood by those within the art that, can be to this The technical scheme of utility model is modified or equivalent substitution, without departing from the objective and model of technical solutions of the utility model Enclose, it all should cover among right of the present utility model.

Claims

A kind of 1. T cell culture dish for being conveniently replaceable culture medium, it is characterised in that：Including interior ware and cover outer outside interior ware Ware, the mouth rim of the interior ware are placed on the oral area end face of outer ware, and interior ware oral area is additionally provided with ware lid；The interior ware Bottom is provided with culture medium via and covers the cell filtering net on culture medium via.
2. the T cell culture dish according to claim 1 for being conveniently replaceable culture medium, it is characterised in that：The bottom of the interior ware Culture medium flowing lumen is provided between portion and the bottom of outer ware, is provided with the side of the outer ware and is connected with culture medium flowing lumen Enter discharging tube, it is described enter discharging tube oral area be provided with seal plug.