CN204644368U - Temperature control device for cell culture under microscope - Google Patents
Temperature control device for cell culture under microscope Download PDFInfo
- Publication number
- CN204644368U CN204644368U CN201520239537.3U CN201520239537U CN204644368U CN 204644368 U CN204644368 U CN 204644368U CN 201520239537 U CN201520239537 U CN 201520239537U CN 204644368 U CN204644368 U CN 204644368U
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- CN
- China
- Prior art keywords
- box body
- microscope
- temperature control
- lid
- temperature
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- Expired - Fee Related
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- 238000004113 cell culture Methods 0.000 title claims abstract description 20
- 238000010438 heat treatment Methods 0.000 claims description 13
- 239000012780 transparent material Substances 0.000 claims description 8
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 230000008676 import Effects 0.000 claims description 3
- 235000019994 cava Nutrition 0.000 claims 1
- 230000010261 cell growth Effects 0.000 abstract description 5
- 238000004088 simulation Methods 0.000 abstract description 3
- 230000007613 environmental effect Effects 0.000 abstract description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 3
- 210000003437 trachea Anatomy 0.000 abstract 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000008054 signal transmission Effects 0.000 description 1
Landscapes
- Microscoopes, Condenser (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a temperature control device for cell culture under microscope, its characterized in that: including air supply, water bath, objective table and temperature controller, the air supply through the trachea with the access connection of water bath, the export of water bath through the trachea with the air inlet of objective table is connected, the signal end of temperature controller with the objective table is connected. The utility model discloses can carry out cell growth environmental simulation in the objective table, whole device simple structure, the volume is less, and the control by temperature change is accurate.
Description
Technical field
The utility model relates to a kind of temperature control unit, is specifically related to cell cultures temperature control unit under a kind of microscope.
Background technology
Cell culture technology is also cell clone technology, for whole bioengineering field, cell cultures is all a requisite process, cell culture technology can be become the many cells of simply unicellular or few differentiation through mass propgation by a cell, or obtain a large amount of cells or its meta-bolites by cell cultures, because biological product all gets from cell, cell culture technology is most crucial in biotechnology, most basic technology thus.
The cultivation general reference vitro culture of cell, its implication refers to takes out tissue in living animal body, and in analogue body in the specific body such as physiological environment under condition, carry out cell culture growth, wherein envrionment temperature is an extremely important condition element of cell cultures.Be at present all be placed in special culture dish to carry out for cell cultures, then be placed in temperature control unit by culture dish and carry out temperature control adjustment, simulation physiological environment, so that Growth of Cells.But these above-mentioned temperature control units involve great expense mostly, volume is also comparatively large, and mobile inconvenience, especially under lab, often need to observe cell growth status under the microscope, this temperature control unit is obviously just not suitable for employing.
Summary of the invention
It is simple that the utility model object is to provide a kind of structure, small volume, and cell cultures temperature control unit under the accurate microscope of temperature control.
The technical solution of the utility model is: cell cultures temperature control unit under a kind of microscope, comprise source of the gas, water-bath, Stage microscope and temperature controller, described source of the gas is connected through the import of tracheae with described water-bath, the outlet of described water-bath is connected through the inlet mouth of tracheae with described Stage microscope, and the signal end of described temperature controller is connected with described Stage microscope.
Further technical scheme, described Stage microscope is made up of box body and lid, cave inward in the middle part of described box body and be provided with cavity volume, the first temperature sensor is provided with above described cavity volume, described box body wherein a side are provided with the second temperature sensor, the signal end of first and second temperature sensor described is connected with described temperature controller, and described tray interior is provided with heating unit, and the control end of described heating unit is connected with described temperature controller;
Spaced apartly on the end face of described lid just to described box body be provided with several production wells, on the end face of described lid back to described box body, inlet mouth is offered with the conducting of described air outlet, described inlet mouth is connected with the outlet of described water-bath, and the periphery that described lid and described box body cover rear described lid matches with the periphery of described box body.
Further technical scheme, adopts transparent material to form sightingpiston bottom described cavity volume.
Further technical scheme, adopts transparent material to form sightingpiston in the middle of described lid.
Further technical scheme, described heating unit is heater strip.
The utility model has the advantages that:
1. the utility model comprises source of the gas, water-bath, Stage microscope and temperature controller, gas exports from source of the gas and arrive Stage microscope after water-bath, the culture dish having cell is placed in Stage microscope, temperature controller controls the heating element heats of carrier interior and monitors temperature, thus Growth of Cells environmental simulation is carried out in Stage microscope, whole apparatus structure is simple, small volume, and temperature control is accurate;
2. Stage microscope small volume in the utility model, and the middle part of the bottom of box body content cavity and lid is all transparent setting, conveniently observes the Growth of Cells in Stage microscope under the microscope;
3. the utility model by arranging two temperature sensors respectively above box body sidepiece and box body content cavity, can complete simultaneously monitor while Stage microscope temperature and cell sample temperature, thus can monitor temperature more accurately;
4. the utility model structure is simple, easy to use, is easy to realize, and cost is not high, is applicable to promoting the use of.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the utility model is further described:
Fig. 1 is the structured flowchart of the utility model embodiment one;
Fig. 2 is the structural representation of the box body of Stage microscope in the utility model embodiment one;
Fig. 3 is that the lid of Stage microscope in the utility model embodiment one is just to the structural representation of box body one side;
Fig. 4 is the structural representation of box body back to box body one side of Stage microscope in the utility model embodiment one.
Wherein: 1, box body; 2, lid; 3, cavity volume; 4, the first temperature sensor; 5, the second temperature sensor; 6, heating unit; 7, production well; 8, inlet mouth; 9, sightingpiston.
Embodiment
Embodiment one: see shown in Fig. 1 to 4, cell cultures temperature control unit under a kind of microscope, comprise source of the gas, water-bath, Stage microscope and temperature controller, described source of the gas is connected through the import of tracheae with described water-bath, the outlet of described water-bath is connected through the inlet mouth of tracheae with described Stage microscope, and the signal end of described temperature controller is connected with described Stage microscope.
Described Stage microscope is made up of box body 1 and lid 2, cave inward in the middle part of described box body 1 and be provided with cavity volume 3, transparent material is adopted to form sightingpiston bottom described cavity volume, the preferred synthetic glass of transparent material, the first temperature sensor 4 is provided with above described cavity volume 3, described box body 1 wherein a side are provided with the second temperature sensor 5, the signal end of first and second temperature sensor described is connected with described temperature controller, described tray interior is provided with heating unit 6, described heating unit is heater strip, and the control end of described heating unit is connected with described temperature controller;
Spaced apartly on the end face of described lid 2 just to described box body 1 be provided with several production wells 7, on the end face of described box body, inlet mouth 8 is offered at described lid 1 with described air outlet 7 conducting, described inlet mouth is connected with the outlet of described water-bath, the periphery that described lid and described box body cover rear described lid matches with the periphery of described box body, transparent material is adopted to form sightingpiston 9, the preferred synthetic glass of transparent material in the middle of described lid 2.
The utility model in use, carbon dioxide exports in source of the gas, water-bath is entered through tracheae, water-bath is mainly used in distillation in laboratory, drying, concentrates, also can be used for thermostatically heating and other humid test, gas in water-bath out after inlet mouth through tracheae, lid and air outlet be drained in Stage microscope, temperature controller controls the heating element heats in box body, thus analog cell growing environment, the culture dish now depositing cell is placed in the cavity volume of box body.Above the cavity volume of box body and the side of box body be respectively equipped with first and second temperature sensor, two temperature sensors are monitored the temperature of Stage microscope and cell sample simultaneously and Signal transmissions are returned temperature controller, the real-time monitoring temperature of temperature controller can conveniently regulating and controlling.Stage microscope small volume, and be transparent setting bottom box body and in the middle part of lid, be convenient to be placed on below microscope and cell is observed.
Certain above-described embodiment, only for technical conceive of the present utility model and feature are described, its object is to person skilled in the art can be understood content of the present utility model and implement according to this, can not limit protection domain of the present utility model with this.All modifications done according to the spirit of the utility model main technical schemes, all should be encompassed within protection domain of the present utility model.
Claims (5)
1. cell cultures temperature control unit under a microscope, it is characterized in that: comprise source of the gas, water-bath, Stage microscope and temperature controller, described source of the gas is connected through the import of tracheae with described water-bath, the outlet of described water-bath is connected through the inlet mouth of tracheae with described Stage microscope, and the signal end of described temperature controller is connected with described Stage microscope.
2. cell cultures temperature control unit under a kind of microscope according to claim 1, it is characterized in that: described Stage microscope is made up of box body (1) and lid (2), described box body (1) middle part caves inward and is provided with cavity volume (3), described cavity volume (3) top is provided with the first temperature sensor (4), described box body (1) wherein a side are provided with the second temperature sensor (5), the signal end of first and second temperature sensor described is connected with described temperature controller, described box body (1) inside is provided with heating unit (6), the control end of described heating unit is connected with described temperature controller,
Spaced apartly on the end face of described lid (2) just to described box body (1) be provided with several production wells (7), on the end face of described box body (1), inlet mouth (8) is offered at described lid (2) with described air outlet (7) conducting, described inlet mouth is connected with the outlet of described water-bath, and the periphery that described lid and described box body cover rear described lid matches with the periphery of described box body.
3. cell cultures temperature control unit under a kind of microscope according to claim 2, is characterized in that: adopt transparent material to form sightingpiston bottom described cavity volume.
4. cell cultures temperature control unit under a kind of microscope according to claim 2, is characterized in that: adopt transparent material to form sightingpiston (9) in the middle of described lid (2).
5. cell cultures temperature control unit under a kind of microscope according to claim 2, is characterized in that: described heating unit is heater strip.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201520239537.3U CN204644368U (en) | 2015-04-20 | 2015-04-20 | Temperature control device for cell culture under microscope |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN201520239537.3U CN204644368U (en) | 2015-04-20 | 2015-04-20 | Temperature control device for cell culture under microscope |
Publications (1)
Publication Number | Publication Date |
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CN204644368U true CN204644368U (en) | 2015-09-16 |
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Family Applications (1)
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CN201520239537.3U Expired - Fee Related CN204644368U (en) | 2015-04-20 | 2015-04-20 | Temperature control device for cell culture under microscope |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107338186A (en) * | 2016-05-03 | 2017-11-10 | 华北理工大学 | Microscope cell cultivation equipment |
CN107908205A (en) * | 2017-11-13 | 2018-04-13 | 浙江师范大学 | Constant temperature observation device |
-
2015
- 2015-04-20 CN CN201520239537.3U patent/CN204644368U/en not_active Expired - Fee Related
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107338186A (en) * | 2016-05-03 | 2017-11-10 | 华北理工大学 | Microscope cell cultivation equipment |
CN107908205A (en) * | 2017-11-13 | 2018-04-13 | 浙江师范大学 | Constant temperature observation device |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150916 Termination date: 20180420 |